CN107586701B

CN107586701B - Reaction bin and molecular hybridization instrument

Info

Publication number: CN107586701B
Application number: CN201711021918.4A
Authority: CN
Inventors: 陈吾科; 韦亨勇; 刘毅聪; 李丽贞
Original assignee: Lbp Medicine Science & Technology Co ltd
Current assignee: Lbp Medicine Science & Technology Co ltd
Priority date: 2017-10-26
Filing date: 2017-10-26
Publication date: 2020-05-19
Anticipated expiration: 2037-10-26
Also published as: CN107586701A

Abstract

The invention discloses a reaction bin and a molecular hybridization instrument, wherein the reaction bin comprises a plurality of unit bins arranged in parallel, each unit bin is provided with an independent reaction cavity, the reaction cavities are arranged vertically, a plurality of groups of elastic injection ports and air pressure balance ports are arranged at the upper part of the reaction bin, the injection ports and the air pressure balance ports are in a closed state in a natural state, and each unit bin corresponds to one group of elastic injection ports and air pressure balance ports. The reaction bin can be applied to a molecular hybridization instrument, the volume of the molecular hybridization instrument is reduced, the working space is saved, the reaction bin is simple in structure, each unit bin is provided with an independent reaction cavity, test paper can be placed and observed conveniently, the arrangement of the injection opening and the air pressure balance opening can ensure the air pressure balance of the reaction cavity, and the injection opening and the air pressure balance opening are in a closed state in a natural state, so that the liquid in the reaction cavity flows out or overflows when the reaction bin rotates, and the molecular hybridization instrument can be better subjected to experiments and reactions.

Description

Reaction bin and molecular hybridization instrument

Technical Field

The invention relates to the technical field of molecular hybridization, in particular to a reaction bin applicable to a molecular hybridization instrument and the molecular hybridization instrument applying the reaction bin.

Background

Molecular hybridization apparatus, also known as: molecular hybridization box and molecular hybridization oven, which are widely used in the aspects of screening cloned genes, making enzyme cutting maps, qualitative and quantitative detection of specific gene sequences in genomes, diagnosis of diseases and the like. Molecular hybridization instruments are not only widely used in the field of molecular biology, but also increasingly used in clinical diagnosis.

The basic principle of molecular hybridization is to use the denaturation and renaturation of nucleic acid molecules to make DNA (or RNA) fragments with different sources form a hybrid double-stranded molecule (heteroduplex) according to the base complementary relationship, and the hybrid double-stranded can be formed between DNA and DNA strands or between RNA and DNA strands.

With the wide application and popularization of molecular hybridization instruments, the use requirements of people on the molecular hybridization instruments are higher and higher, the existing molecular hybridization instruments do not have carriers used for placing test paper independently, the test paper is generally horizontally placed in a reaction area of the molecular hybridization instrument during an experiment, and for the molecular hybridization instrument capable of carrying out a high-flux experiment, the reaction area of the molecular hybridization instrument is larger due to the need of carrying out more sample detection experiments, so that the size of the molecular hybridization instrument is larger, and the molecular hybridization instrument occupies more working space; the test paper is flatly placed in a reaction area of the molecular hybridization instrument, the test paper needs to be singly taken and placed for alignment, great inconvenience is brought to work of people, and the experimental effect is often not ideal enough.

Disclosure of Invention

The reaction bin can be applied to the molecular hybridization instrument, the size of the molecular hybridization instrument is reduced, the working space is saved, the reaction bin is simple in structure, the test paper can be placed and observed conveniently, and the reaction bin can have a good experiment effect.

The technical scheme is as follows:

the molecular hybridization instrument comprises a frame body, wherein a suction head placing part, a sample placing part, a reaction part, a control manipulator and a manipulator movement driving mechanism are arranged on the frame body,

the suction head placing part is provided with a plurality of suction head placing cavities;

the sample placing part is provided with a plurality of sample placing cavities;

the reaction part is provided with a rotating bin and a reaction bin, the rotating bin is rotatably arranged in the rack body and provided with a placing cavity, the reaction bin is arranged in the placing cavity, and the reaction bin is provided with a reaction cavity;

the control manipulator is provided with a suction head operating mechanism, a liquid feeding and discharging operating mechanism and a control mechanism, and the suction head operating mechanism and the liquid feeding and discharging operating mechanism are connected with the control mechanism;

the manipulator is provided with a suction head loading station, a sample liquid sucking station, a sample liquid injecting station and a liquid feeding and draining station, and the suction head loading station corresponds to the suction head placing part; the sample liquid sucking station corresponds to the sample placing part; the sample liquid injection station and the liquid adding and discharging station correspond to the reaction part; the manipulator movement driving mechanism is in driving connection with the control manipulator and drives the control manipulator to move to each station.

The rack body is also provided with a waste collecting part, and the waste collecting part is provided with a waste collecting cavity; the manipulator is also provided with a suction head unloading station which corresponds to the waste collecting part.

The rack body is provided with a mounting concave position, the rack body is internally provided with a rotary driving mechanism, the outer end parts of two opposite sides of the rotary bin are respectively provided with a rotary shaft, the rotary bin is arranged at the mounting concave position of the rack body, and the rotary driving mechanism is circumferentially connected with the rotary shafts of the rotary bin.

The reaction bin comprises a plurality of unit bins arranged in parallel, each unit bin is provided with an independent reaction cavity, the reaction cavities are vertically arranged, the upper parts of the reaction bins are provided with a plurality of groups of elastic injection ports and air pressure balance ports, the injection ports and the air pressure balance ports are in a closed state in a natural state, and each unit bin corresponds to one group of elastic injection ports and air pressure balance ports.

And an anti-reverse bulge is arranged on one side of the reaction bin.

The reaction bin comprises a bin main body and a bin cover, the bin cover is arranged on the bin main body, the bin main body is provided with a plurality of unit bins which are arranged in parallel, and the plurality of groups of elastic injection ports and the air pressure balance ports are arranged on the bin cover.

The unit bins are provided with a first side part and a second side part, the first side part of each unit bin is positioned on the same side, and the second side part of each unit bin is positioned on the same side;

the unit bins are divided into a first unit bin, a second unit bin, … and an nth unit bin, the first unit bin and the second unit bin are connected through a second side portion, the second unit bin and the third unit bin are connected through a first side portion, the third unit bin and the fourth unit bin are connected through a second side portion, and so on.

The opposite sides of the first unit bin and the second unit bin are respectively provided with corresponding tearing parts, the tearing parts are arranged close to the first sides of the first unit bin and the second unit bin, and the tearing parts on the first unit bin and the second unit bin are connected;

the opposite sides of the second unit bin and the third unit bin are respectively provided with corresponding tearing parts, the tearing parts are arranged close to the second sides of the second unit bin and the third unit bin, and the tearing parts on the second unit bin and the third unit bin are connected;

the opposite sides of the third unit bin and the fourth unit bin are respectively provided with corresponding tearing parts, the tearing parts are arranged close to the first sides of the third unit bin and the fourth unit bin, and the tearing parts on the third unit bin and the fourth unit bin are connected;

and so on.

The tearing parts are arranged at the upper end and the lower end of the side part of each unit bin, and the connected tearing parts form a tearing concave part facing the outside of the reaction bin.

The test paper test device is characterized in that a partition plate is arranged in the unit bin, the partition plate divides a reaction cavity of the unit bin into a test paper placing cavity and a liquid adding and discharging cavity, and the test paper placing cavity is communicated with the liquid adding and discharging cavity.

The partition plates comprise a first partition plate and a second partition plate, and the first partition plate and the second partition plate are respectively arranged on two opposite side parts of the unit bin and are arranged in a staggered mode.

And anti-sticking wall convex parts are respectively arranged on the opposite side walls of the test paper placing cavity.

The anti-sticking wall protrusion is an anti-sticking wall convex strip arranged along the height direction of the side wall of the test paper placing cavity; two sticking-proof wall raised lines are respectively arranged on the opposite side walls of the test paper placing cavity.

At least two of the side parts of the unit bin are observation side parts which are transparent.

The molecular hybridization instrument comprises a rack body, wherein a reaction part is arranged on the rack body, the reaction part is provided with a rotating bin and the reaction bin, the rotating bin is rotatably arranged in the rack body, the rotating bin is provided with a placement cavity, and the reaction bin is arranged in the placement cavity.

The suction head operating mechanism comprises a vertical translation guide plate, a suction head assembling and disassembling pipe and a suction head disassembling baffle plate,

the suction head assembling and disassembling pipes are arranged on the vertical translation guide plate and are provided with assembling and disassembling parts which are positioned below the vertical translation guide plate;

the suction head detachment baffle is correspondingly provided with a plurality of through holes, the suction head detachment baffle is arranged below the vertical translation guide plate, and the assembling and disassembling part of the suction head assembling and disassembling pipe penetrates through the through holes in the suction head detachment baffle;

the control mechanism comprises a first vertical translation driving mechanism and an air pressure control mechanism,

the first vertical translation driving mechanism is in driving connection with the sucker operating mechanism and controls the sucker operating mechanism to perform vertical translation movement; when the suction head is unloaded, the first vertical translation driving mechanism controls the vertical translation guide plate to move upwards in a translation manner relative to the suction head unloading baffle;

the upper part of the suction head loading and unloading pipe is provided with a first connecting pipe, and the suction head loading and unloading pipe is connected with the air pressure control mechanism through the first connecting pipe.

The liquid feeding and discharging operating mechanism comprises a lifting guide plate, a liquid feeding and discharging pipe, an air pressure balance pipe, a first limiting plate and a second limiting plate,

the liquid feeding and discharging pipe and the air pressure balancing pipe are provided with a plurality of groups; the plurality of liquid feeding and discharging pipes are arranged on the lifting guide plate, a plurality of through holes are correspondingly formed in the lifting guide plate, and the plurality of air pressure balance pipes correspondingly penetrate through the through holes in the lifting guide plate; the first limiting plate is arranged at the upper part of the air pressure balance pipe, the second limiting plate is arranged at the lower part of the air pressure balance pipe, and the air pressure balance pipe penetrates through the second limiting plate; the lifting guide plate is positioned between the first limiting plate and the second limiting plate in the vertical direction, and the height difference between the first limiting plate and the second limiting plate is smaller than the height of the reaction cavity;

the control mechanism comprises a second vertical translation driving mechanism and a liquid feeding and discharging control mechanism, and the second vertical translation driving mechanism drives the lifting guide plate to move up and down; the liquid feeding and discharging control mechanism is connected with the liquid feeding and discharging pipe and controls the liquid feeding and discharging pipe; when the lifting guide plate does lifting motion, the first limiting plate or the second limiting plate is pushed to drive the air pressure balance pipe to do lifting motion.

The liquid feeding and discharging operation mechanism is provided with a plurality of liquid feeding and discharging pipes, the control manipulator is also provided with a liquid dropping prevention mechanism,

the drip-proof mechanism comprises a liquid containing seat, a plurality of buffer containing cavities are arranged in the liquid containing seat, a plurality of liquid injection ports and liquid outlet ports are correspondingly arranged on the liquid containing seat, and the liquid injection ports and the liquid outlet ports are communicated with the buffer containing cavities;

each liquid injection port is connected with a flow dividing pipe, and the other end of each flow dividing pipe is connected to a flow dividing pipe; the liquid outlets are respectively connected with liquid conveying pipes, and the other end of each liquid conveying pipe is respectively connected with each liquid feeding and discharging pipe.

Each buffering cavity is connected with a diversion pipeline, the other end of the diversion pipeline extends to the liquid outlet, the diversion pipeline is communicated with the liquid outlet, and the caliber of the diversion pipeline is smaller than that of the buffering cavity.

The caliber of the diversion pipeline is 1/4 to 1/3 of the caliber of the buffer cavity.

The diversion pipeline comprises a first vertical portion, a transverse portion and a second vertical portion, one end of the first vertical portion is connected with the buffer containing cavity, the other end of the first vertical portion is connected with one end of the transverse portion downwards, the other end of the transverse portion is connected with the second vertical portion upwards, and the other end of the second vertical portion extends to the liquid outlet.

The sizes of the buffer cavities are consistent.

The buffer cavity and the flow guide pipeline are integrally formed.

The use method of the molecular hybridization instrument comprises the following steps,

a plurality of suckers are placed at the sucker placing part; a plurality of sample tubes are arranged at the sample placing part, and sample liquid is filled in the sample tubes; a plurality of test papers are placed in the reaction cavity of the reaction bin;

the manipulator is controlled to move to a suction head loading station, a sample liquid sucking station, a sample liquid injection station and a liquid adding and discharging station under the drive of the manipulator movement driving mechanism;

when the control manipulator moves to a suction head mounting station, the control mechanism controls the suction head operating mechanism to carry out suction head mounting operation;

when the control manipulator moves to a sample liquid sucking station, the control mechanism controls the suction head operating mechanism to suck sample liquid, and at the moment, the suction head operating mechanism is provided with a suction head;

when the control manipulator moves to a sample liquid injection station, the control mechanism controls the suction head operating mechanism to inject sample liquid into the reaction cavity, and at the moment, the suction head operating mechanism is provided with a suction head, and the suction head absorbs the sample liquid; or the control mechanism controls the liquid feeding and discharging operation mechanism to carry out liquid feeding and discharging operation at the reaction cavity;

the rotary bin rotates, and the liquid in the reaction cavity reacts with the test paper in a rotating state.

The method for using the molecular hybridization instrument further comprises the following steps,

the first vertical translation driving mechanism drives the suction head operating mechanism to do vertical translation movement, and a suction head of the suction head operating mechanism is used for assembling and disassembling a tubular suction head;

the first vertical translation driving mechanism drives the sucker operating mechanism to do vertical translation movement, the air pressure control mechanism controls the sucker operating mechanism to suck sample liquid or inject the sample liquid, and at the moment, a sucker loading and unloading pipe of the sucker operating mechanism is provided with a sucker;

the first vertical translation driving mechanism drives the vertical translation guide plate to move upwards in a translation mode relative to the suction head detachment baffle plate, and the suction head mounted on the suction head mounting and detaching pipe is detached under the blocking of the suction head detachment baffle plate.

the second vertical translation driving mechanism drives the lifting guide plate to move downwards, the liquid feeding and discharging pipe enters the reaction cavity, the second limiting plate moves downwards under the pushing of the lifting guide plate, the second limiting plate drives the air pressure balance pipe to move downwards, and the air pressure balance pipe enters the reaction cavity;

the second vertical translation driving mechanism drives the lifting guide plate to move upwards, the liquid feeding and discharging pipe moves upwards until the liquid feeding and discharging pipe is separated from the reaction cavity, the first limiting plate moves upwards under the pushing of the lifting guide plate, and the first limiting plate drives the air pressure balance pipe to move upwards until the air pressure balance pipe is separated from the reaction cavity;

the liquid feeding and draining control mechanism controls a liquid feeding and draining pipe of the liquid feeding and draining control mechanism to feed or drain liquid.

It should be noted that:

the foregoing "first and second" do not denote any particular quantity or order, but rather are used to distinguish one name from another.

The following illustrates the advantages or principles of the invention:

1. the molecular hybridization instrument is characterized in that a sucker placing part, a sample placing part, a reaction part, a control manipulator and a manipulator movement driving mechanism are arranged on a rack body, the sucker placing part is provided with a plurality of sucker placing cavities, and the sucker placing cavities are used for placing suckers; the sample placing part is provided with a plurality of sample placing cavities, and the sample placing cavities are used for placing samples; the reaction part is provided with a rotary bin and a reaction bin, the rotary bin is rotatably arranged in the rack body, the reaction bin is arranged in a mounting cavity of the rotary bin, the reaction bin is provided with a reaction cavity, and the reaction cavity is used for placing test paper and adding sample liquid and reaction liquid;

the control manipulator is provided with a suction head operating mechanism, a liquid feeding and draining operating mechanism and a control mechanism, and the suction head operating mechanism and the liquid feeding and draining operating mechanism are connected with the control mechanism; the control manipulator is provided with a suction head loading station, a sample liquid sucking station, a sample liquid injecting station and a liquid feeding and draining station, and the suction head loading station corresponds to the suction head placing part; the sample liquid suction station corresponds to the sample placing part; the sample liquid injection station and the liquid adding and discharging station correspond to the reaction part; the manipulator movement driving mechanism is in driving connection with the control manipulator and drives the control manipulator to move to each station;

when the molecular hybridization instrument is used, a plurality of suckers are placed at the sucker placing part; a plurality of sample tubes are arranged at the sample placing part, and sample liquid is filled in the sample tubes; a plurality of test papers are placed in the reaction cavity of the reaction bin; the manipulator is controlled to move to a suction head loading station, a sample liquid sucking station, a sample liquid injection station and a liquid adding and discharging station under the drive of the manipulator movement driving mechanism;

when the control manipulator moves to a suction head mounting station, the control mechanism controls the suction head operating mechanism to carry out suction head mounting operation; when the control manipulator moves to a sample liquid sucking station, the control mechanism controls the suction head operating mechanism to suck sample liquid, and at the moment, the suction head operating mechanism is provided with a suction head; when the control manipulator moves to a sample liquid injection station, the control mechanism controls the suction head operating mechanism to inject sample liquid into the reaction cavity, and at the moment, the suction head operating mechanism is provided with a suction head, and the suction head absorbs the sample liquid; or the control mechanism controls the liquid feeding and discharging operation mechanism to carry out liquid feeding and discharging operation at the reaction cavity; the rotary bin rotates, and the liquid in the reaction cavity reacts with the test paper in a rotating state;

the molecular hybridization instrument is simple in structure and reasonable in structural design, is provided with the suction head placing part, the sample placing part, the reaction part, the control manipulator and the manipulator movement driving mechanism, fully utilizes the space of the rack body, is small in size and can save working space; the reaction part of the molecular hybridization instrument can carry out experiments for detecting more samples, and meets the requirements of people on the molecular hybridization instrument for detecting more experimental samples; the manipulator can respectively carry out the operations of loading the sucker, sucking the sample liquid, injecting the sample liquid and adding liquid and draining liquid at the sucker loading station, the sample liquid sucking station, the sample liquid injecting station and the liquid and draining station, the automation degree is high, and the operation time can be saved for people; rotatory storehouse rotates and sets up in the frame body, and the reaction storehouse has the reaction chamber in locating the arrangement chamber in rotatory storehouse, and this molecular hybridization appearance when the experiment, rotatory storehouse rotates, can improve experiment reaction rate, reduces reaction and experimental time to improve people's work efficiency, the people's of being convenient for work, and can obtain better experiment effect.

2. Still be equipped with waste product collection portion on the frame body, waste product collection portion has waste product collection chamber, controls the manipulator and still has the suction head station of unloading, and the suction head station of unloading corresponds with waste product collection portion, and waste product collection chamber is used for collecting suction head and the waste liquid after using, can play the effect of environmental protection, and the people's of being convenient for work just improves work efficiency better.

3. The frame body is provided with a concave mounting position, the frame body is internally provided with a rotary driving mechanism, the outer end parts of two opposite sides of the rotary bin are provided with a rotary shaft, the rotary bin is arranged at the concave mounting position of the frame body, the rotary driving mechanism is circumferentially connected with the rotary bin, the concave mounting position of the frame body can be better utilized, the rotary bin is driven by the rotary driving mechanism to rotate in a reciprocating manner, and the molecular hybridization instrument can have a better experimental effect during an experiment.

4. The reaction bin comprises a plurality of unit bins arranged in parallel, each unit bin is provided with an independent reaction cavity, the reaction cavities are arranged vertically, the upper part of the reaction bin is provided with a plurality of groups of elastic injection ports and air pressure balance ports, the injection ports and the air pressure balance ports are in a closed state in a natural state, each unit bin corresponds to one group of elastic injection ports and air pressure balance ports, the reaction bin is simple in structure, the structural design greatly compresses the experimental space of the molecular hybridization instrument, so that the overall size of the molecular hybridization instrument is reduced, each unit bin is provided with an independent reaction cavity, the reaction bin is convenient for placing test paper and observing experiments, the work of people is facilitated, the experiments carried out by each reaction cavity are not interfered with each other, the experimental effect of the molecular hybridization instrument is ensured, the arrangement of the injection ports and the air pressure balance ports can ensure the air pressure balance of the reaction cavities, and the air pressure balance ports are in a closed state in a natural state, when the reaction bin rotates, the liquid in the reaction cavity flows out or overflows, and the molecular hybridization instrument can better perform experiments and reactions.

5. The anti-reverse protrusion is arranged on one side of the reaction bin, the anti-reverse concave part is arranged on one side of the wall of the rotary bin, the anti-reverse protrusion corresponds to the anti-reverse concave part, the anti-reverse protrusion and the anti-reverse concave part are arranged, the reaction bin is convenient to mount, the situation of reverse mounting of the reaction bin is avoided, and the experimental efficiency is guaranteed.

6. The reaction bin comprises a bin main body and a bin cover, the bin cover is arranged on the bin main body, the bin main body is provided with a plurality of unit bins which are arranged in parallel, a plurality of groups of elastic injection ports and air pressure balance ports are arranged on the bin cover, the bin main body and the bin cover are separated to be convenient for putting test paper into the bin cover, and the operation of an experiment is realized.

7. The unit storehouse has first lateral part, the second lateral part, the unit storehouse is divided into first unit storehouse, the second unit storehouse, …, nth unit storehouse, first unit storehouse, the second unit storehouse links to each other through the second lateral part, the second unit storehouse, the third unit storehouse links to each other through first lateral part, the third unit storehouse, the fourth unit storehouse links to each other through the second lateral part, analogize in proper order, the connection structure in unit storehouse is convenient for the expansion in experiment back unit storehouse, the unit storehouse can expand into one row under this connection structure, the observation after the experiment of ten minutes being convenient for.

8. The opposite sides of the first unit bin and the second unit bin are respectively provided with corresponding tearing parts, the tearing parts are arranged close to the first sides of the first unit bin and the second unit bin, and the tearing parts on the first unit bin and the second unit bin are connected; the opposite sides of the second unit bin and the third unit bin are respectively provided with corresponding tearing parts, the tearing parts are arranged close to the second sides of the second unit bin and the third unit bin, and the tearing parts on the second unit bin and the third unit bin are connected; the opposite sides of the third unit bin and the fourth unit bin are respectively provided with corresponding tearing parts, the tearing parts are arranged close to the first sides of the third unit bin and the fourth unit bin, and the tearing parts on the third unit bin and the fourth unit bin are connected; and so on; the arrangement of the tearing part ensures that all the unit bins of the reaction bin can be stably arranged in parallel, and the separation between the unit bins after the experiment is convenient.

9. The tearing part is arranged at the upper end and the lower end of the side part of each unit bin, the connected tearing parts form a tearing concave part facing the outside of the reaction bin, the setting position of the tearing part can save materials and cost, the effect of stable and parallel arrangement of the unit bins is further ensured, and the setting of the tearing concave part can facilitate the separation between the unit bins after the experiment.

10. Be equipped with the baffle in the unit storehouse, the baffle divide into the test paper with the reaction chamber in unit storehouse and places chamber and liquid feeding flowing back chamber, and the test paper is placed the chamber and is connected mutually with liquid feeding flowing back chamber, and the test paper has solitary chamber of placing, can guarantee the effect of experiment better, improves the efficiency of the experiment.

11. The baffle includes first baffle and second baffle, and first baffle, second baffle are located respectively on the double-phase opposite side portion in unit storehouse, and crisscross setting, and first baffle and second baffle can effectively avoid the test paper to enter into liquid feeding flowing back chamber, and have guaranteed effectively that the test paper places the chamber and liquid feeding flowing back chamber looks UNICOM.

12. The test paper is placed and is equipped with respectively on the relative lateral wall in chamber and prevent pasting the wall convex part, prevents pasting the setting of wall convex part and can avoid the test paper to paste on placing the lateral wall in chamber when the experiment, has eliminated the influence of test paper adherence to the experimental result.

13. The anti-sticking wall protrusion is an anti-sticking wall convex strip arranged along the height direction of the side wall of the test paper placing cavity; two on the relative lateral wall in chamber is placed to the test paper respectively prevent pasting the wall sand grip, prevent pasting setting up of wall sand grip can be better prevent that the test paper from pasting in the lateral wall of placing the chamber to ensure the effect of preventing sticking to the wall.

14. The lateral part in unit storehouse has two at least for observing the lateral part, observes the lateral part and is transparent, and the people of being convenient for observes the experimental result through observing the lateral part.

15. The sucker operating mechanism comprises a vertical translation guide plate, sucker loading and unloading pipes and sucker unloading baffles, the sucker loading and unloading pipes are arranged on the vertical translation guide plate and are provided with loading and unloading parts, and the loading and unloading parts are positioned below the vertical translation guide plate; the suction head detachment baffle is correspondingly provided with a plurality of through holes, the suction head detachment baffle is arranged below the vertical translation guide plate, and the loading and unloading part of the suction head loading and unloading pipe penetrates through the through holes on the suction head detachment baffle;

the control mechanism comprises a first vertical translation driving mechanism and an air pressure control mechanism, the first vertical translation driving mechanism is in driving connection with the suction head operating mechanism, a first connecting pipe is arranged at the upper part of the suction head loading and unloading pipe, and the suction head loading and unloading pipe is connected with the air pressure control mechanism through the first connecting pipe;

when the control manipulator works, the first vertical translation driving mechanism drives the suction head operating mechanism to do vertical translation movement, and a suction head of the suction head operating mechanism is used for assembling and disassembling a tubular suction head; the first vertical translation driving mechanism drives the sucker operating mechanism to do vertical translation movement, the air pressure control mechanism controls the sucker operating mechanism to suck sample liquid or inject the sample liquid, and at the moment, a sucker loading and unloading pipe of the sucker operating mechanism is provided with a sucker; the first vertical translation driving mechanism drives the vertical translation guide plate to move upwards in a translation manner relative to the suction head detachment baffle plate, and the suction head arranged on the suction head assembly and disassembly pipe is disassembled under the blocking of the suction head detachment baffle plate;

this control manipulator simple structure, and structural design is reasonable, and its powerful can realize dress suction head, absorb sample liquid, injection sample liquid and unload the operation of suction head, and degree of automation is high, can be better save operating time, has reduced artificial operation as far as possible, has improved work efficiency.

16. The liquid feeding and discharging operating mechanism comprises a lifting guide plate, a liquid feeding and discharging pipe, an air pressure balance pipe, a first limiting plate and a second limiting plate, wherein the liquid feeding and discharging pipe and the air pressure balance pipe are provided with a plurality of groups; the plurality of liquid feeding and discharging pipes are arranged on the lifting guide plate, a plurality of through holes are correspondingly formed in the lifting guide plate, and the plurality of air pressure balance pipes correspondingly penetrate through the through holes in the lifting guide plate; the first limiting plate is arranged at the upper part of the air pressure balance pipe, the second limiting plate is arranged at the lower part of the air pressure balance pipe, and the air pressure balance pipe penetrates through the second limiting plate; the lifting guide plate is positioned between the first limiting plate and the second limiting plate in the vertical direction, and the height difference between the first limiting plate and the second limiting plate is smaller than the height of the reaction cavity;

the control mechanism comprises a second vertical translation driving mechanism and a liquid feeding and discharging control mechanism, and the second vertical translation driving mechanism drives the lifting guide plate to move up and down; the liquid feeding and discharging control mechanism is connected with the liquid feeding and discharging pipe and controls the liquid feeding and discharging pipe;

when the control manipulator works, the second vertical translation driving mechanism drives the lifting guide plate to move downwards, the liquid feeding and discharging pipe enters the reaction cavity, the second limiting plate moves downwards under the pushing of the lifting guide plate, the second limiting plate drives the air pressure balance pipe to move downwards, and the air pressure balance pipe enters the reaction cavity; the second vertical translation driving mechanism drives the lifting guide plate to move upwards, the liquid feeding and discharging pipe moves upwards until the liquid feeding and discharging pipe is separated from the reaction cavity, the first limiting plate moves upwards under the pushing of the lifting guide plate, and the first limiting plate drives the air pressure balance pipe to move upwards until the air pressure balance pipe is separated from the reaction cavity; the liquid feeding and discharging control mechanism controls a liquid feeding and discharging pipe of the liquid feeding and discharging control mechanism to feed liquid or discharge liquid;

this control manipulator with liquid feeding and the collection of draining liquid phase, simplified the structure of current manipulator for this control manipulator's structure is more simple, and occupation space diminishes, and should control the manipulator and can be when the liquid feeding flowing back, balanced atmospheric pressure avoids this control manipulator when carrying out the liquid feeding flowing back, the condition of dropping liquid appears, and then influences the experiment.

17. The liquid feeding and discharging operation mechanism is provided with a plurality of liquid feeding and discharging pipes, the control manipulator is also provided with an anti-dripping mechanism, the anti-dripping mechanism comprises a liquid containing seat, a plurality of buffer containing cavities are arranged in the liquid containing seat, a plurality of liquid injection ports and liquid outlet ports are correspondingly arranged on the liquid containing seat, and the liquid injection ports and the liquid outlet ports are communicated with the buffer containing cavities; each liquid injection port is connected with a flow dividing pipe, and the other end of each flow dividing pipe is connected to a flow dividing pipe; the liquid outlets are respectively connected with liquid conveying pipes, and the other ends of the liquid conveying pipes are respectively connected with liquid feeding and discharging pipes;

this drip proof mechanism simple structure, and excellent in use effect, it is provided with the buffering and holds the chamber, has effectively avoided the appearance of liquid dropping liquid condition when shunting, can also effectively avoid leading to the appearance of the backward flow condition between the reposition of redundant personnel pipe because of hydraulic pressure is uneven when liquid shunts.

18. Each buffering cavity is connected with a diversion pipeline, the other end of the diversion pipeline extends to the liquid outlet, the diversion pipeline is communicated with the liquid outlet, the caliber of the diversion pipeline is smaller than that of the buffering cavity, and the arrangement of the diversion pipeline further ensures the use effect of the drip-proof mechanism.

19. The aperture of the diversion pipeline is 1/4 to 1/3 of the aperture of the buffer containing cavity, the aperture of the diversion pipeline is set to be optimal, the aperture of the diversion pipeline is 1/4 to 1/3 of the aperture of the buffer containing cavity, and the drip-proof mechanism has the optimal use effect.

20. The water conservancy diversion pipeline includes first vertical portion, horizontal portion and the vertical portion of second, and the buffering appearance chamber is connected to the one end of first vertical portion, and the one end of horizontal portion is connected downwards to the other end, and the vertical portion of ascending second is connected to the other end of horizontal portion, and the other end of the vertical portion of second extends to liquid outlet department, and the water conservancy diversion mode of water conservancy diversion pipeline makes this drip proof mechanism have better result of use.

21. The sizes of the buffer cavities are consistent, and the buffering effect can be better ensured.

22. The buffer containing cavity and the flow guide pipeline are integrally formed, so that the liquid containing seat is convenient to process and produce, and the production cost is saved.

Drawings

FIG. 1 is a schematic perspective view of a molecular hybridization apparatus according to an embodiment of the present invention;

FIG. 2 is a schematic top view of the interior of a molecular hybridization apparatus according to an embodiment of the present invention;

FIG. 3 is a schematic side view of a reaction part according to an embodiment of the present invention;

FIG. 4 is a schematic view showing an exploded structure of a reaction part according to an embodiment of the present invention;

FIG. 5 is a schematic top view of a reaction part according to an embodiment of the present invention;

FIG. 6 is a schematic perspective view of a reaction chamber body according to an embodiment of the present invention;

FIG. 7 is a schematic side view of a reaction chamber body according to an embodiment of the present invention;

FIG. 8 is a schematic top view of a reaction chamber body according to an embodiment of the present invention;

FIG. 9 is a schematic structural view of a reaction cartridge body according to an embodiment of the present invention when deployed;

FIG. 10 is a schematic structural diagram of a manipulator robot according to an embodiment of the present invention;

FIG. 11 is one of the schematic views of the liquid adding and discharging operation mechanism according to the embodiment of the present invention;

FIG. 12 is a second schematic diagram of the liquid feeding and discharging operation mechanism according to the embodiment of the present invention;

FIG. 13 is a third schematic diagram of the liquid feeding and discharging operation mechanism according to the embodiment of the present invention;

FIG. 14 is a fourth schematic diagram illustrating the use of a liquid adding and draining mechanism according to an embodiment of the present invention;

FIG. 15 is a fifth schematic diagram of the liquid feeding and discharging operation mechanism according to the embodiment of the present invention;

FIG. 16 is a schematic side view of the inner structure of the drip proof mechanism according to the embodiment of the present invention;

FIG. 17 is a schematic view of a part of the structure of an anti-dripping mechanism according to an embodiment of the present invention;

description of reference numerals:

10. a rack body, 11, a sucker placing part, 12, a sample placing part, 13, a reaction part, 131, a rotating bin, 1311, a placing cavity, 1312, a rotating shaft, 1313, an anti-reverse concave part, 132, a reaction bin, 1321, a bin main body, 1322, a bin cover, 1323, a unit bin, 1324, an injection port, 1325, an air pressure balancing port, 1326, an anti-reverse convex part, 1327, a tearing part, 1328, a tearing concave part, 1329, a test paper placing cavity, 1330, a liquid feeding and discharging cavity, 1331, an anti-wall sticking convex part, 1332, an observation side part, 14, a waste collecting part, 15, an installation concave part, 20, a control mechanical hand, 21, a sucker operating mechanism, 211, a vertical translation guide plate, 212, a loading and unloading pipe, 213, a sucker removing baffle, 22, a liquid feeding and discharging operating mechanism, 221, a lifting guide plate, 222, a liquid feeding and discharging pipe, 223, an air pressure balancing pipe, 224, a first limiting plate, 225, a second limiting plate, 23 and a first vertical translation driving mechanism, 24. the device comprises a second vertical translation driving mechanism, 25, a drip-proof mechanism, 251, a liquid containing seat, 252, a buffer containing cavity, 253, a liquid injection port, 254, a liquid outlet, 255, a flow dividing pipe, 256, a flow collecting pipe, 257 and a flow guide pipeline.

Detailed Description

The following provides a detailed description of embodiments of the invention.

As shown in FIGS. 1 to 17, the molecular hybridization apparatus includes a rack body 10, a tip placing part 11, a sample placing part 12, a reaction part 13, a waste collecting part 14, a manipulator 20 and a manipulator movement driving mechanism are provided on the rack body 10,

the suction head placing part 11 is provided with a plurality of suction head placing cavities; the specimen-placement section 12 has a plurality of specimen placement chambers; the reaction part 13 has a rotary bin 131 and a reaction bin 132, the rotary bin 131 is rotatably disposed in the rack body 10, the rotary bin 131 has a placing cavity 1311, the reaction bin 132 is disposed in the placing cavity 1311, and the reaction bin 132 has a reaction cavity; the waste collection portion 14 has a waste collection chamber;

the control manipulator 20 is provided with a sucker operating mechanism 21, a liquid feeding and discharging operating mechanism 22 and a control mechanism, and the sucker operating mechanism 21 and the liquid feeding and discharging operating mechanism 22 are connected with the control mechanism;

the control manipulator 20 is provided with a suction head loading station, a sample liquid sucking station, a sample liquid injection station, a liquid feeding and discharging station and a suction head unloading station, wherein the suction head loading station corresponds to the suction head placing part 11; the sample liquid suction station corresponds to the sample placing part 12; the sample liquid injection station and the liquid adding and discharging station correspond to the reaction part 13; the suction head unloading station corresponds to the waste collecting part 14; the manipulator movement driving mechanism is in driving connection with the control manipulator 20 and drives the control manipulator 20 to move to each station.

Wherein, the rack body 10 is provided with a mounting concave position 15, the rack body 10 is internally provided with a rotary driving mechanism, the outer end parts of two opposite sides of the rotary bin 131 are respectively provided with a rotary shaft 1312, the rotary bin 131 is arranged at the mounting concave position 15 of the rack body 10, and the rotary driving mechanism is circumferentially connected with the rotary shaft 1312 of the rotary bin 131;

the reaction bin 132 comprises a plurality of unit bins 1323 arranged in parallel, each unit bin 1323 is provided with an independent reaction cavity, the reaction cavities are vertically arranged, the upper part of the reaction bin 132 is provided with a plurality of groups of elastic injection ports 1324 and air pressure balance ports 1325, the injection ports 1324 and the air pressure balance ports 1325 are in a closed state in a natural state, and each unit bin 1323 corresponds to one group of elastic injection ports 1324 and air pressure balance ports 1325; in this embodiment, the reaction bin 132 has a plurality of reaction sub-bins, and the reaction sub-bins include a plurality of unit bins 1323 arranged in parallel;

an anti-reverse protrusion 1326 is arranged on one side of the reaction bin 132, an anti-reverse recess 1313 is arranged on one side of the bin wall of the rotary bin 131, and the anti-reverse protrusion 1326 corresponds to the anti-reverse recess 1313;

the reaction bin 132 comprises a bin main body 1321 and a bin cover 1322, the bin cover 1322 is covered on the bin main body 1321, the bin main body 1321 is provided with a plurality of unit bins 1323 which are arranged in parallel, and a plurality of groups of elastic injection ports 1324 and air pressure balancing ports 1325 are arranged on the bin cover 1322;

the unit bins 1323 have a first side portion and a second side portion, the first side portion of each unit bin 1323 is located on the same side, and the second side portion of each unit bin 1323 is located on the same side; the unit bin 1323 is divided into a first unit bin, a second unit bin, … and an nth unit bin, the first unit bin and the second unit bin are connected through a second side part, the second unit bin and the third unit bin are connected through a first side part, the third unit bin and the fourth unit bin are connected through a second side part, and so on;

the opposite sides of the first unit bin and the second unit bin are respectively provided with corresponding tearing parts 1327, the tearing parts 1327 are arranged close to the first sides of the first unit bin and the second unit bin, and the tearing parts 1327 on the first unit bin and the second unit bin are connected;

the opposite sides of the second unit bin and the third unit bin are respectively provided with corresponding tearing parts 1327, the tearing parts 1327 are arranged close to the second sides of the second unit bin and the third unit bin, and the tearing parts 1327 on the second unit bin and the third unit bin are connected;

the opposite sides of the third unit bin and the fourth unit bin are respectively provided with corresponding tearing parts 1327, the tearing parts 1327 are arranged close to the first sides of the third unit bin and the fourth unit bin, and the tearing parts 1327 on the third unit bin and the fourth unit bin are connected; and so on;

the tearing portions 1327 are provided at the upper and lower ends of the side portion of each unit cell 1323, and the connected tearing portions 1327 form a tearing concave portion 1328 facing the outside of the reaction cell 132;

a partition board is arranged in the unit bin 1323, the reaction cavity of the unit bin 1323 is divided into a test paper placing cavity 1329 and a liquid adding and discharging cavity 1330 by the partition board, and the test paper placing cavity 1329 is communicated with the liquid adding and discharging cavity 1330; in this embodiment, the partition plates include a first partition plate and a second partition plate, which are respectively disposed on two opposite sides of the unit bin 1323 and are staggered;

anti-sticking wall protrusions 1331 are respectively arranged on the opposite side walls of the test paper placing cavity 1329, in the embodiment, the anti-sticking wall protrusions are anti-sticking wall protruding strips arranged along the height direction of the side walls of the test paper placing cavity 1329, and two anti-sticking wall protruding strips are respectively arranged on the opposite side walls of the test paper placing cavity 1329;

at least two of the sides of the unit cell 1323 are viewing sides 1332, and the viewing sides 1332 are transparent;

the suction head operating mechanism 21 comprises a vertical translation guide plate 211, a suction head loading and unloading pipe 212 and a suction head detachment baffle plate 213,

the suction head assembling and disassembling pipes 212 are multiple, the suction head assembling and disassembling pipes 212 are arranged on the vertical translation guide plate 211, the suction head assembling and disassembling pipes 212 are provided with assembling and disassembling parts, and the assembling and disassembling parts are positioned below the vertical translation guide plate 211; the suction head detachment baffle 213 is correspondingly provided with a plurality of through holes, the suction head detachment baffle 213 is arranged below the vertical translation guide plate 211, and the loading and unloading part of the suction head loading and unloading pipe 212 penetrates through the through holes on the suction head detachment baffle 213;

the control mechanism comprises a first vertical translation driving mechanism 23 and an air pressure control mechanism, the first vertical translation driving mechanism 23 is in driving connection with the sucker operating mechanism 21, and the sucker operating mechanism 21 is controlled to perform vertical translation movement; when the suction head is unloaded, the first vertical translation driving mechanism 23 controls the vertical translation guide plate 211 to move in a translation manner upwards relative to the suction head unloading baffle plate 213; the upper part of the suction head assembling and disassembling pipe 212 is provided with a first connecting pipe, and the suction head assembling and disassembling pipe 212 is connected with the air pressure control mechanism through the first connecting pipe;

the liquid feeding and discharging operation mechanism 22 comprises a lifting guide plate 221, a liquid feeding and discharging pipe 222, an air pressure balancing pipe 223, a first limit plate 224 and a second limit plate 225,

the liquid feeding and discharging pipe 222 and the air pressure balancing pipe 223 have a plurality of groups; a plurality of liquid feeding and discharging pipes 222 are arranged on the lifting guide plate 221, a plurality of through holes are correspondingly arranged on the lifting guide plate 221, and a plurality of air pressure balancing pipes 223 correspondingly penetrate through the through holes on the lifting guide plate 221; the first limit plate 224 is arranged at the upper part of the air pressure balancing pipe 223, the second limit plate 225 is arranged at the lower part of the air pressure balancing pipe 223, and the air pressure balancing pipe 223 penetrates through the second limit plate 225; the lifting guide plate 221 is located between the first limit plate 224 and the second limit plate 225 in the vertical direction, and the height difference between the first limit plate 224 and the second limit plate 225 is smaller than the height of the reaction chamber;

the control mechanism comprises a second vertical translation driving mechanism 24 and a liquid feeding and discharging control mechanism, and the second vertical translation driving mechanism 24 drives the lifting guide plate 221 to move up and down; the liquid feeding and discharging control mechanism is connected with the liquid feeding and discharging pipe 222 to control the liquid feeding and discharging; when the lifting guide plate 221 moves up and down, the first limit plate 224 or the second limit plate 225 is pushed to drive the air pressure balance pipe 223 to move up and down;

the liquid feeding and discharging operation mechanism 22 is provided with a plurality of liquid feeding and discharging pipes 222, the control manipulator 20 is further provided with an anti-dripping mechanism 25, the anti-dripping mechanism 25 comprises a liquid containing seat 251, a plurality of buffer containing cavities 252 are arranged in the liquid containing seat 251, a plurality of liquid injection ports 253 and liquid outlet ports 254 are correspondingly arranged on the liquid containing seat 251, and the liquid injection ports 253 and the liquid outlet ports 254 are communicated with the buffer containing cavities 252; the liquid injection ports 253 are respectively connected with a flow dividing pipe 255, and the other end of each flow dividing pipe 255 is respectively connected to a flow dividing pipe 256; the liquid outlets 254 are respectively connected with liquid conveying pipes, and the other ends of the liquid conveying pipes are respectively connected with the liquid feeding and discharging pipes 222;

each buffer cavity 252 is connected with a flow guide pipeline 257, the other end of the flow guide pipeline 257 extends to the liquid outlet 254, the flow guide pipeline 257 is communicated with the liquid outlet 254, and the aperture of the flow guide pipeline 257 is smaller than that of the buffer cavity 252; preferably, the aperture of the diversion pipeline 257 is 1/4 to 1/3 of the aperture of the buffer cavity 252, the sizes of the buffer cavities 252 are the same, and the buffer cavities 252 and the diversion pipeline 257 are integrally formed;

diversion pipe 257 includes first vertical portion, horizontal portion and second vertical portion, and buffer cavity 252 is connected to the one end of first vertical portion, and the other end is connected the one end of horizontal portion downwards, and the other end of horizontal portion is connected the second vertical portion of ascending, and the other end of second vertical portion extends to liquid outlet 254 department.

a plurality of suckers are placed at the sucker placing part 11; a plurality of sample tubes are arranged at the sample placing part 12, and sample liquid is filled in the sample tubes; a plurality of test papers are placed in the reaction cavity of the reaction bin 132;

the control manipulator 20 is driven by the manipulator movement driving mechanism to move to a suction head loading station, a sample liquid sucking station, a sample liquid injection station, a liquid feeding and discharging station and a suction head unloading station;

when the control manipulator 20 moves to a suction head loading station, the control mechanism controls the suction head operating mechanism 21 to load the suction head;

when the control manipulator 20 moves to a sample liquid sucking station, the control mechanism controls the suction head operating mechanism 21 to suck sample liquid, and at the moment, the suction head operating mechanism 21 is provided with a suction head;

when the control manipulator 20 moves to a sample liquid injection station, the control mechanism controls the suction head operating mechanism 21 to perform sample liquid injection operation at the reaction cavity, at this time, the suction head operating mechanism 21 is provided with a suction head, and the suction head sucks sample liquid; or, the control mechanism controls the liquid feeding and discharging operation mechanism 22 to perform liquid feeding and discharging operation at the reaction chamber;

when the manipulator 20 is controlled to move to a suction head unloading station, the control mechanism controls the suction head operating mechanism 21 to unload the suction head, and at the moment, the suction head operating mechanism 21 is provided with the suction head;

the rotating bin 131 rotates, and the liquid in the reaction cavity reacts with the test paper in a rotating state.

The use method of the molecular hybridization instrument further comprises the following steps,

the first vertical translation driving mechanism 23 drives the sucker operating mechanism 21 to do vertical translation movement, and the sucker loading and unloading pipe 212 of the sucker operating mechanism 21 loads the sucker;

the first vertical translation driving mechanism 23 drives the sucker operating mechanism 21 to do vertical translation movement, the air pressure control mechanism controls the sucker operating mechanism 21 to suck sample liquid or inject the sample liquid, and at the moment, the sucker loading and unloading pipe 212 of the sucker operating mechanism 21 is provided with a sucker;

the first vertical translation driving mechanism 23 drives the vertical translation guide plate 211 to move in a translation manner upward relative to the tip removing baffle 213, and the tip mounted on the tip mounting and dismounting pipe 212 is dismounted under the blocking of the tip removing baffle 213.

the second vertical translation driving mechanism 24 drives the lifting guide plate 221 to move downwards, the liquid feeding and discharging pipe 222 enters the reaction chamber, the second limiting plate 225 is pushed by the lifting guide plate 221 to move downwards, the second limiting plate 225 drives the air pressure balancing pipe 223 to move downwards, and the air pressure balancing pipe 223 enters the reaction chamber;

the second vertical translation driving mechanism 24 drives the lifting guide plate 221 to move upwards, the liquid adding and discharging pipe 222 moves upwards until the liquid adding and discharging pipe is separated from the reaction cavity, the first limiting plate 224 moves upwards under the pushing of the lifting guide plate 221, and the first limiting plate 224 drives the air pressure balancing pipe 223 to move upwards until the air pressure balancing pipe 223 is separated from the reaction cavity;

the liquid feeding and discharging control mechanism operates the liquid feeding and discharging pipe 222 of the liquid feeding and discharging control mechanism to feed or discharge liquid.

The advantages of this embodiment:

1. a molecular hybridization instrument is characterized in that a sucker placing part 11, a sample placing part 12, a reaction part 13, a waste collecting part 14, a control manipulator 20 and a manipulator movement driving mechanism are arranged on a rack body 10, the sucker placing part 11 is provided with a plurality of sucker placing cavities, and the sucker placing cavities are used for placing suckers; the specimen-placing section 12 has a plurality of specimen-placing chambers for placing a specimen; the reaction part 13 is provided with a rotary bin 131 and a reaction bin 132, the rotary bin 131 is rotatably arranged in the rack body 10, the reaction bin 132 is arranged in a placing cavity 1311 of the rotary bin 131, the reaction bin 132 is provided with a reaction cavity, and the reaction cavity is used for placing test paper and adding sample liquid and reaction liquid; the waste collecting part 14 is provided with a waste collecting cavity for collecting the used suction head and waste liquid;

the control manipulator 20 is provided with a sucker operating mechanism 21, a liquid feeding and discharging operating mechanism 22 and a control mechanism, and the sucker operating mechanism 21 and the liquid feeding and discharging operating mechanism 22 are connected with the control mechanism; the control manipulator 20 is provided with a suction head loading station, a sample liquid sucking station, a sample liquid injection station, a liquid feeding and discharging station and a suction head unloading station, wherein the suction head loading station corresponds to the suction head placing part 11; the sample liquid suction station corresponds to the sample placing part 12; the sample liquid injection station and the liquid adding and discharging station correspond to the reaction part 13; the suction head unloading station corresponds to the waste collecting part 14; the manipulator movement driving mechanism is in driving connection with the control manipulator 20 and drives the control manipulator 20 to move to each station;

when the molecular hybridization instrument is used, a plurality of suckers are placed at the sucker placing part 11; a plurality of sample tubes are arranged at the sample placing part 12, and sample liquid is filled in the sample tubes; a plurality of test papers are placed in the reaction cavity of the reaction bin 132; the control manipulator 20 is driven by the manipulator movement driving mechanism to move to a suction head loading station, a sample liquid sucking station, a sample liquid injection station, a liquid feeding and discharging station and a suction head unloading station;

when the control manipulator 20 moves to a suction head loading station, the control mechanism controls the suction head operating mechanism 21 to load the suction head; when the control manipulator 20 moves to a sample liquid sucking station, the control mechanism controls the suction head operating mechanism 21 to suck sample liquid, and at the moment, the suction head operating mechanism 21 is provided with a suction head; when the control manipulator 20 moves to a sample liquid injection station, the control mechanism controls the suction head operating mechanism 21 to perform sample liquid injection operation at the reaction cavity, at this time, the suction head operating mechanism 21 is provided with a suction head, and the suction head sucks sample liquid; or, the control mechanism controls the liquid feeding and discharging operation mechanism 22 to perform liquid feeding and discharging operation at the reaction chamber; when the manipulator 20 is controlled to move to a suction head unloading station, the control mechanism controls the suction head operating mechanism 21 to unload the suction head, and at the moment, the suction head operating mechanism 21 is provided with the suction head; the rotating bin 131 rotates, and the liquid in the reaction cavity reacts with the test paper in a rotating state;

the molecular hybridization instrument is simple in structure and reasonable in structural design, is provided with the suction head placing part 11, the sample placing part 12, the reaction part 13, the waste product collecting part 14, the control manipulator 20 and the manipulator movement driving mechanism, fully utilizes the space of the rack body 10, is small in size and can save working space; the reaction part 13 of the molecular hybridization instrument can carry out experiments of detecting more samples, and meets the requirements of people on the molecular hybridization instrument for detecting more experimental samples; the manipulator 20 can respectively carry out the operations of loading the suction head, sucking the sample liquid, injecting the sample liquid, adding liquid and draining liquid and unloading the suction head at a suction head loading station, a sample liquid sucking station, a sample liquid injecting station, a liquid adding and draining station and a suction head unloading station, has high automation degree, and can save the operation time for people; rotatory storehouse 131 rotates and sets up in frame body 10, and reaction storehouse 132 is located in the chamber 1311 of settling of rotatory storehouse 131, and reaction storehouse 132 has the reaction chamber, and this molecular hybridization appearance is when the experiment, and rotatory storehouse 131 rotates, can improve experiment reaction rate, reduces reaction and experimental time to improve people's work efficiency, the work of the people of being convenient for, and can obtain better experimental effect.

2. Be equipped with the concave position 15 of installation on frame body 10, be equipped with rotary driving mechanism in the frame body 10, be equipped with rotation axis 1312 on the outer tip of the double-phase offside of rotatory storehouse 131, the concave position 15 department of installation of frame body 10 is located to rotatory storehouse 131, rotary driving mechanism and rotary driving mechanism 131's rotation axis 1312 circumferential connection, the utilization in frame body 10 space can be reflected better in the setting of the concave position 15 of installation, rotatory storehouse 131 is under rotary driving mechanism's drive, reciprocating rotation, it can have better experimental effect to have guaranteed that this molecular hybridization appearance can have when the experiment.

3. The reaction bin 132 comprises a plurality of unit bins 1323 arranged in parallel, each unit bin 1323 is provided with an independent reaction cavity, the reaction cavity is arranged vertically, the upper part of the reaction bin 132 is provided with a plurality of groups of elastic injection ports 1324 and air pressure balance ports 1325, the injection ports 1324 and the air pressure balance ports 1325 are in a closed state in a natural state, each unit bin 1323 corresponds to one group of elastic injection ports 1324 and air pressure balance ports 1325, the reaction bin 132 is simple in structure, the structural design greatly compresses the experimental space of the molecular hybridization instrument, the overall volume of the molecular hybridization instrument is reduced, each unit bin 1323 is provided with an independent reaction cavity, the reaction bin 132 is convenient for placing test paper and observing experiments, the work of people is facilitated, the experiments carried out by each reaction cavity are not interfered with each other, the experimental effect of the molecular hybridization instrument is ensured, the air pressure balance of the reaction cavities can be ensured by the arrangement of the injection ports 1324 and the air pressure balance ports 1325, the injection port 1324 and the air pressure balance port 1325 are in a closed state in a natural state, so that when the reaction bin 132 rotates, liquid in the reaction cavity flows out or overflows, and the molecular hybridization instrument can better perform experiments and reactions.

4. The anti-reverse protrusion 1326 is arranged on one side of the reaction bin 132, the anti-reverse recess 1313 is arranged on one side of the bin wall of the rotary bin 131, the anti-reverse protrusion 1326 corresponds to the anti-reverse recess 1313, the anti-reverse protrusion 1326 and the anti-reverse recess 1313 are arranged to facilitate installation of the reaction bin 132, the anti-reverse installation of the reaction bin 132 is avoided, and the experiment efficiency is guaranteed.

5. The reaction chamber 132 includes a chamber main body 1321 and a chamber cover 1322, the chamber cover 1322 is covered on the chamber main body 1321, the chamber main body 1321 has a plurality of unit chambers 1323 arranged in parallel, a plurality of groups of elastic injection ports 1324 and air pressure balance ports 1325 are arranged on the chamber cover 1322, and the chamber main body 1321 and the chamber cover 1322 are separately arranged to facilitate the test paper to be put in and the experiment to be operated.

6. Unit storehouse 1323 has first lateral part, the second lateral part, unit storehouse 1323 divide into first unit storehouse, the second unit storehouse, …, nth unit storehouse, first unit storehouse, the second unit storehouse passes through the second lateral part and links to each other, the second unit storehouse, the third unit storehouse passes through first lateral part and links to each other, the third unit storehouse, the fourth unit storehouse passes through the second lateral part and links to each other, analogize in proper order, the connection structure of unit storehouse 1323 is convenient for the expansion of experiment back unit storehouse 1323, unit storehouse 1323 can expand into one row under this connection structure, the observation after the experiment of very being convenient for.

7. The opposite sides of the first unit bin and the second unit bin are respectively provided with corresponding tearing parts 1327, the tearing parts 1327 are arranged close to the first sides of the first unit bin and the second unit bin, and the tearing parts 1327 on the first unit bin and the second unit bin are connected; the opposite sides of the second unit bin and the third unit bin are respectively provided with corresponding tearing parts 1327, the tearing parts 1327 are arranged close to the second sides of the second unit bin and the third unit bin, and the tearing parts 1327 on the second unit bin and the third unit bin are connected; the opposite sides of the third unit bin and the fourth unit bin are respectively provided with corresponding tearing parts 1327, the tearing parts 1327 are arranged close to the first sides of the third unit bin and the fourth unit bin, and the tearing parts 1327 on the third unit bin and the fourth unit bin are connected; and so on; the arrangement of the tearing portion 1327 ensures that the unit bins 1323 of the reaction bin 132 can be stably arranged in parallel, and facilitates the separation of the unit bins 1323 after the experiment.

8. The tearing part 1327 is arranged at the upper end and the lower end of the side part of each unit bin 1323, the connected tearing parts 1327 form a tearing concave part 1328 facing the outside of the reaction bin 132, the arrangement position of the tearing parts 1327 can save materials and cost, the effect of stable and parallel arrangement of the unit bins 1323 is further ensured, and the arrangement of the tearing concave part 1328 can facilitate the separation of the unit bins 1323 after the experiment.

9. The unit bin 1323 is internally provided with a partition board, the reaction cavity of the unit bin 1323 is divided into a test paper placing cavity 1329 and a liquid adding and discharging cavity 1330 by the partition board, the test paper placing cavity 1329 is communicated with the liquid adding and discharging cavity 1330, and the test paper is provided with an independent placing cavity, so that the experimental effect can be better ensured, and the experimental efficiency is improved;

the baffle includes first baffle and second baffle, and first baffle, second baffle are located respectively on the double-phase opposite side portion of unit storehouse 1323, and crisscross the setting, and first baffle and second baffle can effectively avoid the test paper to enter into liquid feeding flowing back chamber 1330, and have guaranteed effectively that the test paper places chamber 1329 and liquid feeding flowing back chamber 1330 looks UNICOM.

10. The opposite side walls of the test paper placing cavity 1329 are respectively provided with an anti-sticking wall convex part 1331, and the anti-sticking wall convex part 1331 can prevent the test paper from sticking to the side wall of the placing cavity during an experiment, so that the influence of the test paper sticking to the wall on the experiment result is eliminated;

the anti-sticking wall protrusion is an anti-sticking wall convex strip arranged along the height direction of the side wall of the test paper placing cavity 1329; two anti-sticking wall sand grips on the relative lateral wall of chamber 1329 are placed to the test paper respectively, and the setting up of anti-sticking wall sand grip can be better prevent that the test paper from pasting in the lateral wall of placing the chamber to ensure the effect of preventing sticking the wall.

11. At least two of the side portions of the unit bin 1323 are observation side portions 1332, and the observation side portions 1332 are transparent, so that people can observe experimental results conveniently by observing the side portions 1332.

12. The suction head operating mechanism 21 comprises a vertical translation guide plate 211, suction head loading and unloading pipes 212 and a suction head detachment baffle 213, wherein the suction head loading and unloading pipes 212 are arranged on the vertical translation guide plate 211, the suction head loading and unloading pipes 212 are provided with loading and unloading parts, and the loading and unloading parts are positioned below the vertical translation guide plate 211; the suction head detachment baffle 213 is correspondingly provided with a plurality of through holes, the suction head detachment baffle 213 is arranged below the vertical translation guide plate 211, and the loading and unloading part of the suction head loading and unloading pipe 212 penetrates through the through holes on the suction head detachment baffle 213;

the control mechanism comprises a first vertical translation driving mechanism 23 and an air pressure control mechanism, the first vertical translation driving mechanism 23 is in driving connection with the suction head operating mechanism 21, a first connecting pipe is arranged at the upper part of the suction head assembling and disassembling pipe 212, and the suction head assembling and disassembling pipe 212 is connected with the air pressure control mechanism through the first connecting pipe;

when the control manipulator 20 works, the first vertical translation driving mechanism 23 drives the sucker operating mechanism 21 to do vertical translation movement, and the sucker loading and unloading pipe 212 of the sucker operating mechanism 21 loads suckers; the first vertical translation driving mechanism 23 drives the sucker operating mechanism 21 to do vertical translation movement, the air pressure control mechanism controls the sucker operating mechanism 21 to suck sample liquid or inject the sample liquid, and at the moment, the sucker loading and unloading pipe 212 of the sucker operating mechanism 21 is provided with a sucker; the first vertical translation driving mechanism 23 drives the vertical translation guide plate 211 to move in a translation manner upwards relative to the suction head detachment baffle 213, and the suction head arranged on the suction head mounting and dismounting pipe 212 is dismounted under the blocking of the suction head detachment baffle 213;

this control manipulator 20 simple structure, and structural design is reasonable, and its powerful can realize dress suction head, absorb sample liquid, injection sample liquid and unload the operation of suction head, and degree of automation is high, can be better save operating time, has reduced artificial operation as far as possible, has improved work efficiency.

13. The liquid feeding and discharging operating mechanism 22 comprises a lifting guide plate 221, a liquid feeding and discharging pipe 222, an air pressure balancing pipe 223, a first limiting plate 224 and a second limiting plate 225, wherein the liquid feeding and discharging pipe 222 and the air pressure balancing pipe 223 are provided with a plurality of groups; a plurality of liquid feeding and discharging pipes 222 are arranged on the lifting guide plate 221, a plurality of through holes are correspondingly arranged on the lifting guide plate 221, and a plurality of air pressure balancing pipes 223 correspondingly penetrate through the through holes on the lifting guide plate 221; the first limit plate 224 is arranged at the upper part of the air pressure balancing pipe 223, the second limit plate 225 is arranged at the lower part of the air pressure balancing pipe 223, and the air pressure balancing pipe 223 penetrates through the second limit plate 225; the lifting guide plate 221 is located between the first limit plate 224 and the second limit plate 225 in the vertical direction, and the height difference between the first limit plate 224 and the second limit plate 225 is smaller than the height of the reaction chamber;

the control mechanism comprises a second vertical translation driving mechanism 24 and a liquid feeding and discharging control mechanism, and the second vertical translation driving mechanism 24 drives the lifting guide plate 221 to move up and down; the liquid feeding and discharging control mechanism is connected with the liquid feeding and discharging pipe 222 to control the liquid feeding and discharging;

when the manipulator 20 works, the second vertical translation driving mechanism 24 drives the lifting guide plate 221 to move downwards, the liquid feeding and discharging pipe 222 enters the reaction chamber, the second limiting plate 225 is pushed by the lifting guide plate 221 to move downwards, the second limiting plate 225 drives the air pressure balancing pipe 223 to move downwards, and the air pressure balancing pipe 223 enters the reaction chamber; the second vertical translation driving mechanism 24 drives the lifting guide plate 221 to move upwards, the liquid adding and discharging pipe 222 moves upwards until the liquid adding and discharging pipe is separated from the reaction cavity, the first limiting plate 224 moves upwards under the pushing of the lifting guide plate 221, and the first limiting plate 224 drives the air pressure balancing pipe 223 to move upwards until the air pressure balancing pipe 223 is separated from the reaction cavity; the liquid feeding and discharging control mechanism controls a liquid feeding and discharging pipe 222 of the liquid feeding and discharging control mechanism to feed or discharge liquid;

this control manipulator 20 with liquid feeding and the collection of liquid drainage phase, simplified the structure of current manipulator for this control manipulator 20's structure is simpler, and occupation space diminishes, and should control manipulator 20 can be when the liquid feeding flowing back, and balanced atmospheric pressure avoids this control manipulator 20 when carrying out the liquid feeding flowing back, the condition of dropping liquid appears, and then influences the experiment.

14. The liquid feeding and discharging operation mechanism 22 is provided with a plurality of liquid feeding and discharging pipes 222, the control manipulator 20 is further provided with an anti-dripping mechanism 25, the anti-dripping mechanism 25 comprises a liquid containing seat 251, a plurality of buffer containing cavities 252 are arranged in the liquid containing seat 251, a plurality of liquid injection ports 253 and liquid outlet ports 254 are correspondingly arranged on the liquid containing seat 251, and the liquid injection ports 253 and the liquid outlet ports 254 are communicated with the buffer containing cavities 252; the liquid injection ports 253 are respectively connected with a flow dividing pipe 255, and the other end of each flow dividing pipe 255 is respectively connected to a flow dividing pipe 256; the liquid outlets 254 are respectively connected with liquid conveying pipes, and the other ends of the liquid conveying pipes are respectively connected with the liquid feeding and discharging pipes 222;

this drip proof mechanism 25 simple structure, and excellent in use effect, it is provided with buffering chamber 252, has effectively avoided the appearance of liquid dropping liquid condition when shunting, can also effectively avoid leading to the appearance of the condition of refluxing between shunt tubes 255 because of hydraulic pressure is unbalanced when liquid shunts.

15. Each buffer cavity 252 is connected with a flow guide pipeline 257, the other end of the flow guide pipeline 257 extends to the liquid outlet 254, the flow guide pipeline 257 is communicated with the liquid outlet 254, the aperture of the flow guide pipeline 257 is smaller than that of the buffer cavity 252, and the arrangement of the flow guide pipeline 257 further ensures the use effect of the drip-proof mechanism 25;

the aperture of the diversion pipeline 257 is 1/4 to 1/3 of the aperture of the buffer cavity 252, the aperture of the diversion pipeline 257 is preferably set, the aperture is 1/4 to 1/3 of the aperture of the buffer cavity 252, and the drip-proof mechanism 25 has the optimal use effect;

the sizes of the buffer cavities 252 are consistent, so that the buffering effect can be better ensured;

the buffer cavity 252 and the flow guide pipe 257 are integrally formed, so that the processing and the production of the liquid containing seat 251 are facilitated, and the production cost is saved.

16. The diversion pipeline 257 comprises a first vertical portion, a transverse portion and a second vertical portion, one end of the first vertical portion is connected with the buffer containing cavity 252, the other end of the first vertical portion is connected with one end of the transverse portion downwards, the other end of the transverse portion is connected with the second vertical portion upwards, the other end of the second vertical portion extends to the liquid outlet 254, and the diversion mode of the diversion pipeline 257 enables the drip-proof mechanism 25 to have a better using effect.

The above are merely specific embodiments of the present invention, and the scope of the present invention is not limited thereby; any alterations and modifications without departing from the spirit of the invention are within the scope of the invention.

Claims

1. The reaction bin is characterized by comprising a plurality of unit bins arranged in parallel, each unit bin is provided with an independent reaction cavity, the reaction cavities are vertically arranged, the upper part of each reaction bin is provided with a plurality of groups of elastic injection ports and air pressure balancing ports, the injection ports and the air pressure balancing ports are in a closed state in a natural state, and each unit bin corresponds to one group of elastic injection ports and air pressure balancing ports; a partition board is arranged in the unit bin, the partition board divides a reaction cavity of the unit bin into a test paper placing cavity and a liquid adding and discharging cavity, and the test paper placing cavity is communicated with the liquid adding and discharging cavity; anti-sticking wall convex parts are respectively arranged on the opposite side walls of the test paper placing cavity, and the anti-sticking wall convex parts are anti-sticking wall convex strips arranged along the height direction of the side walls of the test paper placing cavity; two sticking-proof wall raised lines are respectively arranged on the opposite side walls of the test paper placing cavity.

2. The reaction chamber according to claim 1, wherein an anti-reverse protrusion is provided on one side of the reaction chamber.

3. The reaction chamber of claim 1, wherein the reaction chamber comprises a chamber body and a chamber cover, the chamber cover is disposed on the chamber body, the chamber body has the plurality of unit chambers disposed in parallel, and the plurality of sets of elastic injection ports and air pressure balancing ports are disposed on the chamber cover.

4. The reaction chamber of claim 1, wherein the unit chambers have a first side portion and a second side portion, the first side portion of each unit chamber is located on the same side, and the second side portion of each unit chamber is located on the same side;

5. The reaction chamber according to claim 4, wherein the first unit chamber and the second unit chamber are provided with corresponding tearing portions at opposite sides thereof, the tearing portions are disposed near the first sides of the first unit chamber and the second unit chamber, and the tearing portions of the first unit chamber and the second unit chamber are connected;

and so on.

6. The reaction chamber according to claim 5, wherein the tearing portions are provided at upper and lower ends of the side portion of each unit chamber, and the connected tearing portions form a tearing concave portion facing the outside of the reaction chamber.

7. The reaction chamber according to claim 1, wherein the partition plates comprise a first partition plate and a second partition plate, and the first partition plate and the second partition plate are respectively disposed on two opposite sides of the unit chamber and are staggered.

8. The reaction chamber of claim 1, wherein at least two of the sides of the unit chamber are viewing sides, and the viewing sides are transparent.

9. The molecular hybridization instrument is characterized by comprising a rack body, wherein a reaction part is arranged on the rack body, the reaction part is provided with a rotating bin and the reaction bin of any one of the preceding claims, the rotating bin is rotatably arranged in the rack body, the rotating bin is provided with a placement cavity, and the reaction bin is arranged in the placement cavity.