CN213680653U - Gene sequencing instrument sample pretreatment device - Google Patents

Gene sequencing instrument sample pretreatment device Download PDF

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Publication number
CN213680653U
CN213680653U CN202022380146.7U CN202022380146U CN213680653U CN 213680653 U CN213680653 U CN 213680653U CN 202022380146 U CN202022380146 U CN 202022380146U CN 213680653 U CN213680653 U CN 213680653U
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China
Prior art keywords
pipe
broken
crushing
box
connecting rod
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Expired - Fee Related
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CN202022380146.7U
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Chinese (zh)
Inventor
戴争妍
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Changzhou Lvzhen Biotechnology Co ltd
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Changzhou Lvzhen Biotechnology Co ltd
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Priority to CN202022380146.7U priority Critical patent/CN213680653U/en
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Abstract

The embodiment of the utility model discloses gene sequencing instrument sample preprocessing device belongs to sample processing technology field, including broken case, broken bottom of the case portion is provided with the conveyer pipe, conveyer pipe one end is provided with the separator box, broken incasement portion is provided with broken washing mechanism, the inside separating mechanism that is provided with of separator box, broken washing mechanism is including setting up a plurality of baffles in broken incasement portion, broken incasement portion is located the baffle bottom and is provided with the head rod, the connecting rod outside is provided with a plurality of first broken balls, and is a plurality of broken ball surface all is provided with first scraper blade, the head rod bottom is provided with the pivot. The utility model discloses it is rational in infrastructure, can effectually carry out the breakage with cell material, washing, multiple processes such as separation make cell material broken more abundant, improve the treatment effeciency of sample to work efficiency has further been accelerated.

Description

Gene sequencing instrument sample pretreatment device
Technical Field
The embodiment of the utility model provides a relate to sample processing technology field, concretely relates to gene sequencing instrument sample preprocessing device.
Background
DNA sequencing refers to the analysis of the base sequence of a specific DNA fragment, i.e., the arrangement of adenine, thymine, cytosine and guanine, the emergence of rapid DNA sequencing methods has greatly facilitated the research and discovery of biology and medicine, and has made DNA sequence knowledge indispensable in basic biology research and in numerous application fields, such as diagnosis, biotechnology, forensic biology, biosystems, and has made it possible to achieve the rapid sequencing speed of modern DNA sequencing technologies to complete DNA sequences or to complete DNA sequences of various types of genome sequencing and living species, including human genomes and many other animal, plant and microbial species
Before gene sequencing, samples generally need to be processed in aspects of crushing, washing, separating and the like, the processing method is complicated, the samples need to be processed by various instruments, the price is high, the processing time is long, and the efficiency is low.
SUMMERY OF THE UTILITY MODEL
Therefore, the embodiment of the utility model provides a gene sequencing instrument sample preprocessing device to solve among the prior art because the processing method is more loaded down with trivial details, need handle through multiple instrument, the problem of the inefficiency that the processing time is long and lead to moreover.
In order to achieve the above object, the embodiment of the present invention provides the following technical solutions: a sample pretreatment device of a gene sequencing instrument comprises a crushing box, wherein a conveying pipe is arranged at the bottom of the crushing box, a separation box is arranged at one end of the conveying pipe, a crushing and washing mechanism is arranged in the crushing box, and a separation mechanism is arranged in the separation box;
the crushing and washing mechanism comprises a plurality of baffles arranged inside the crushing box, a first connecting rod is arranged at the bottom of the baffles inside the crushing box, a plurality of first crushing balls are arranged outside the first connecting rod, a first scraper blade is arranged on the surface of each first crushing ball, the bottom of the first connecting rod is provided with a rotating shaft, a plurality of stirring rods are arranged outside the rotating shaft, one end of the rotating shaft is provided with a first driving motor, a second connecting rod is arranged at the bottom of the rotating shaft, a plurality of second crushing balls are arranged outside the second connecting rod, a second scraper blade is arranged on the surface of each second crushing ball, a bearing tray is arranged at the bottom of the second connecting rod in the crushing box, a connecting outer pipe is arranged at the bottom of the bearing tray, a first filter screen is arranged in the bearing tray, and a liquid containing tank is arranged at one end of the crushing box;
the separating mechanism comprises a stirring cylinder arranged inside the separating box, a rotating plate is arranged at the bottom of the stirring cylinder, a rotating rod is arranged at the bottom of the rotating plate, a second driving motor is arranged at the bottom end of the rotating rod, a connecting pipe is arranged inside the stirring cylinder, a pump is arranged at one end of the connecting pipe, a processing box is arranged at one end of the pump, a transmission pipe is arranged at the output end of the pump, a second filter screen is arranged inside the processing box, and a liquid discharge pipe is arranged inside the processing box and positioned at the bottom of the second filter screen.
Further, broken roof portion is provided with the blowing fill, the baffle slope sets up at broken incasement lateral wall, flourishing fluid reservoir top is provided with the blowing fill, flourishing fluid reservoir is inside to be provided with the conveying pipe, conveying pipe one end extends to inside the broken incasement portion, the conveying pipe outside is provided with first connecting valve.
Further, first scraper blade and second scraper blade cross sectional shape all set up to trapezoidal, head rod and second connecting rod are the parallel direction setting, pivot one end extends to first driving motor's output, first driving motor can dismantle with broken case lateral wall and be connected.
Further, conveyer pipe one end extends to inside connecting the outer tube, the conveyer pipe other end extends to inside the churn, churn and rotor plate fixed connection.
Furthermore, bull stick one end extends to the second driving motor output, connecting pipe one end can be dismantled with the pump machine input and be connected.
Furthermore, a sealing gasket is arranged at the bottom of the first filter screen inside the bearing tray and made of rubber materials.
Furthermore, one end of the transmission pipe extends to the inside of the treatment box, a liquid inlet hole is formed in the top of the treatment box, the liquid discharge pipe extends to the bottom of the separation box, and a second connecting valve is arranged outside the liquid discharge pipe.
The embodiment of the utility model provides a have following advantage:
1. by arranging the crushing and separating mechanism, the first driving motor is utilized to drive the rotating shaft to rotate and drive the stirring rod to rotate through the rotating shaft, so that cell substances are stirred and crushed, the cell substances are extruded between the stirring rod and the first scraper blade and the second scraper blade, the cell substance crushing effect is better, the cell substances are effectively crushed into fluid, flushing fluid in the liquid containing tank is transmitted to the inside of the crushing tank through the conveying pipe and is used for washing the crushed cell substances, the washing process of the cell substances is completed, the processing progress of the cell substances is accelerated, samples do not need to be processed through various instruments, the processing time is accelerated, and the processing efficiency is improved;
2. through setting up separating mechanism, drive the bull stick through second driving motor and rotate, the bull stick pivoted drives the carousel simultaneously and rotates and drive the churn through the carousel and rotate, make the inside sample of churn be in high-speed running state and separate the material in the sample through the centrifugation, it carries the processing case with the good sample of separation to use the pump machine, add the adsorption liquid with the sample in the processing case, transmit and utilize through the fluid-discharge tube at last, further the processing speed of sample has been accelerated, and the work efficiency is improved, therefore, the clothes hanger is strong in practicability.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It should be apparent that the drawings in the following description are merely exemplary, and that other embodiments can be derived from the drawings provided by those of ordinary skill in the art without inventive effort.
The structure, ratio, size and the like shown in the present specification are only used for matching with the content disclosed in the specification, so as to be known and read by people familiar with the technology, and are not used for limiting the limit conditions which can be implemented by the present invention, so that the present invention has no technical essential significance, and any structure modification, ratio relationship change or size adjustment should still fall within the scope which can be covered by the technical content disclosed by the present invention without affecting the efficacy and the achievable purpose of the present invention.
Fig. 1 is a schematic view of the overall structure of the present invention;
fig. 2 is an enlarged schematic structural view of a point a in fig. 1 according to the present invention;
FIG. 3 is a schematic view of the junction between the treatment tank and the connecting pipe of the present invention;
fig. 4 is a front view of the present invention;
in the figure: 1. a crushing box; 2. a delivery pipe; 3. a separation tank; 4. a baffle plate; 5. a first connecting rod; 6. a first crushing ball; 7. a first squeegee; 8. a rotating shaft; 9. a stirring rod; 10. a first drive motor; 11. a second connecting rod; 12. a second crushing ball; 13. a second squeegee; 14. a receiving tray; 15. connecting an outer pipe; 16. a first filter screen; 17. a liquid containing tank; 18. a mixing drum; 19. a rotating plate; 20. a rotating rod; 21. a second drive motor; 22. a connecting pipe; 23. a pump machine; 24. a treatment tank; 25. a conveying pipe; 26. a second filter screen; 27. a liquid discharge pipe; 28. a transfer tube; 29. and a liquid inlet hole.
Detailed Description
The present invention is described in terms of specific embodiments, and other advantages and benefits of the present invention will become apparent to those skilled in the art from the following disclosure. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to the attached figures 1-4 of the specification, the device for pretreating the gene sequencing instrument sample comprises a crushing box 1, wherein a conveying pipe 2 is arranged at the bottom of the crushing box 1, a separation box 3 is arranged at one end of the conveying pipe 2, a crushing and washing mechanism is arranged inside the crushing box 1, and a separation mechanism is arranged inside the separation box 3.
Broken washing mechanism is including setting up a plurality of baffles 4 in broken case 1 inside, broken case 1 inside is located baffle 4 bottom and is provided with head rod 5, head rod 5 outside is provided with a plurality of first broken balls 6, a plurality of first broken ball 6 surfaces all are provided with first scraper blade 7, head rod 5 bottom is provided with pivot 8, 8 outsides in pivot are provided with a plurality of puddlers 9, 8 one end in pivot is provided with first driving motor 10, 8 bottoms in pivot are provided with second connecting rod 11, 11 outsides in second connecting rod are provided with a plurality of second broken balls 12, a plurality of second broken ball 12 surfaces all are provided with second scraper blade 13, broken case 1 inside is located 11 bottoms in second connecting rod and is provided with accepts tray 14, accept tray 14 bottom and be provided with and connect outer tube 15, accept tray 14 inside is provided with first filter screen 16, broken case 1 one end is provided with flourishing fluid reservoir 17.
As shown in the attached drawing 1, the top of the crushing box 1 is provided with a discharging hopper, the baffle plate 4 is obliquely arranged on the inner side wall of the crushing box 1, the top of the liquid containing tank 17 is provided with a liquid discharging hopper, the inside of the liquid containing tank 17 is provided with a conveying pipe 28, one end of the conveying pipe 28 extends to the inside of the crushing box 1, and the outside of the conveying pipe 28 is provided with a first connecting valve so as to put cell substances into the inside of the crushing box 1 and process the cell substances through the liquid containing tank 17.
As shown in the attached figure 2, the cross sections of the first scraper 7 and the second scraper 13 are both trapezoidal, the first connecting rod 5 and the second connecting rod 11 are arranged in parallel, one end of the rotating shaft 8 extends to the output end of the first driving motor 10, and the first driving motor 10 is detachably connected with the outer side wall of the crushing box 1, so that cell substances to be crushed can be crushed
As shown in fig. 1, a sealing gasket is disposed inside the receiving tray 14 at the bottom of the first filter screen 16, and the sealing gasket is made of rubber, so that the first filter screen 16 can be attached to the receiving tray 14 more conveniently, and untimely filtering can be prevented.
The real-time scene is specifically as follows: when in use, firstly, cell substances needing to be crushed and washed enter the crushing box 1 through the discharge hopper, the cell substances needing to be crushed and washed enter the bottom of the crushing box 1 through the baffle 4, and the baffle 4 is obliquely arranged on the inner side wall of the crushing box 1, so that the cell substances can conveniently slide to the bottom of the inner cavity of the crushing box 1 through the baffle 4, the cell substances can impact the baffle 4 in the sliding process, the cell substances can be primarily crushed, the first driving motor 10 is started, the first driving motor 10 drives the rotating shaft 8 at one end to rotate and drives the stirring rod 9 to rotate through the rotating shaft 8, the cell substances are stirred and crushed, in the stirring process, the cell substances can contact the first crushing balls 6 at the two sides of the stirring rod 9 and the first scraper 7 and the second scraper 13 on the surface of the second crushing balls 12, and when the cell substances are extruded between the stirring rod 9 and the first scraper 7 and the second scraper 13, make cell material crushing effect better, effectually become the fluid with the cell material breakage, accomplish the primary treatment of sample, through opening first connecting valve, make the inside flush fluid of flourishing fluid reservoir 17 enter into broken case 1 inside through conveying pipe 28 and wash the cell material of broken completion, accomplish the washing process of cell material, the cell fluid that has washd enters into through first filter screen 16 and accepts tray 14 on, it is inside to enter into churn 18 through conveyer pipe 2 at last, so that carry out the measure of processing on next step, and is rational in infrastructure, effectively carry out broken washing processing with the cell material, and the operation is convenient.
The separating mechanism comprises a mixing drum 18 arranged inside the separating box 3, a rotating plate 19 is arranged at the bottom of the mixing drum 18, a rotating rod 20 is arranged at the bottom of the rotating plate 19, a second driving motor 21 is arranged at the bottom end of the rotating rod 20, a connecting pipe 22 is arranged inside the mixing drum 18, a pump 23 is arranged at one end of the connecting pipe 22, a processing box 24 is arranged at one end of the pump 23, a transmission pipe 25 is arranged at the output end of the pump 23, a second filter screen 26 is arranged inside the processing box 24, and a liquid discharge pipe 27 is arranged inside the processing box 24 and located at the bottom of the second filter screen.
As shown in FIG. 1, one end of the delivery pipe 2 extends to the inside of the connecting outer pipe 15, the other end of the delivery pipe 2 extends to the inside of the mixing drum 18, and the mixing drum 18 is fixedly connected with a rotating plate 19 so as to deliver the crushed and washed cell materials into the mixing drum 18.
As shown in FIGS. 1 and 4, one end of the rotary rod 20 extends to the output end of the second driving motor 21, and one end of the connecting pipe 22 is detachably connected to the input end of the pump 23, so as to crush the cell material and transmit the sample to be processed through the pump 23.
As shown in the attached figures 1 and 3, one end of the transmission pipe 25 extends into the processing box 24, the top of the processing box 24 is provided with a liquid inlet hole 29, the liquid discharge pipe 27 extends to the bottom of the separation box 3, the outside of the liquid discharge pipe 27 is provided with a second connecting valve so as to facilitate the transmission of processed samples, and the second connecting valve is used for controlling the flow rate of the samples.
The real-time scene is specifically as follows: after cell substances are crushed and washed, the cell substances enter the stirring cylinder 18 through the conveying pipe 2, the second driving motor 21 is started to enable the second driving motor 21 to drive the rotating rod 20 to rotate, the rotating rod 20 drives the rotating plate 19 at the top end to rotate, the rotating plate 19 drives the stirring cylinder 18 fixedly connected with the top to move at a high speed, so that a sample in the stirring cylinder 18 is in a high-speed running state and the substances in the sample are separated through the centrifugal rate, the pump machine 23 is started, the separated substances are conveyed out through the connecting pipe 22 and conveyed into the processing box 24 through the conveying pipe 25 by the pump machine 23, adsorbing liquid is put into the cell substances in the processing box 24 through the liquid inlet hole 29 at the top of the processing box 24, so that the cell substances are adsorbed and mixed, the cell substances are filtered through the second filter screen 26, finally, the second connecting valve is opened, the processed sample is conveyed through the liquid outlet pipe 27 and applied, the separation process of the cellular material is completed.
The utility model discloses use as follows: when in use, firstly, cell substances needing to be crushed and washed enter the crushing box 1 through the discharge hopper, the cell substances can impact the baffle 4 in the process of sliding down, the cell substances can be crushed primarily, the first driving motor 10 is started, the first driving motor 10 drives the rotating shaft 8 at one end to rotate and drives the stirring rod 9 to rotate through the rotating shaft 8, so that the cell substances are stirred and crushed, in the stirring process, the cell substances are extruded between the stirring rod 9 and the first scraper 7 as well as the second scraper 13, by opening the first connecting valve, flushing liquid in the liquid containing tank 17 enters the crushing box 1 through the conveying pipe 28 and washes the crushed cell substances, the cleaned cells enter the stirring cylinder 18 through the conveying pipe 2, the second driving motor 21 is started, the rotating rod 20 is rotated, the rotating rod 20 drives the rotating plate 19 at the top end to rotate, the rotating plate 19 drives the stirring cylinder 18 fixedly connected with the top to move at a high speed, a sample in the stirring cylinder 18 is in a high-speed operation state and substances in the sample are separated through eccentricity, the pump 23 is started, the separated substances are transmitted out through the connecting pipe 22 and are conveyed into the treatment box 24 through the conveying pipe 25 by the pump 23, an adsorption solution is put into cell substances in the treatment box 24, the cell substances are adsorbed and mixed, the cell substances are filtered through the second filter screen 26, finally the second connecting valve is opened, the treated sample is conveyed and applied through the liquid discharge pipe 27, and the separation process of the cell substances is completed, the device has a reasonable structure, and can effectively crush, wash, separate and the like the cell substances, so that the cell substances are crushed more fully, the processing efficiency of the sample is improved, and therefore the working efficiency is further accelerated.
Although the invention has been described in detail with respect to the general description and the specific embodiments, it will be apparent to those skilled in the art that modifications and improvements can be made based on the invention. Therefore, such modifications and improvements are intended to be within the scope of the invention as claimed.
In the present specification, the terms "upper", "lower", "left", "right", "middle", and the like are used for the sake of clarity only, and are not intended to limit the scope of the present invention, and changes or adjustments of the relative relationship thereof are also considered to be the scope of the present invention without substantial changes in the technical content.

Claims (7)

1. The utility model provides a gene sequencing instrument sample preprocessing device, includes broken case (1), its characterized in that: the bottom of the crushing box (1) is provided with a conveying pipe (2), one end of the conveying pipe (2) is provided with a separating box (3), a crushing and washing mechanism is arranged inside the crushing box (1), and a separating mechanism is arranged inside the separating box (3);
the crushing and washing mechanism comprises a plurality of baffle plates (4) arranged inside a crushing box (1), a first connecting rod (5) is arranged inside the crushing box (1) and positioned at the bottom of the baffle plates (4), a plurality of first crushing balls (6) are arranged outside the first connecting rod (5), a first scraper (7) is arranged on the surface of each first crushing ball (6), a rotating shaft (8) is arranged at the bottom of the first connecting rod (5), a plurality of stirring rods (9) are arranged outside the rotating shaft (8), a first driving motor (10) is arranged at one end of the rotating shaft (8), a second connecting rod (11) is arranged at the bottom of the rotating shaft (8), a plurality of second crushing balls (12) are arranged outside the second connecting rod (11), a second scraper (13) is arranged on the surface of each second crushing ball (12), and a bearing tray (14) is arranged inside the crushing box (1) and positioned at the bottom of the second connecting rod (11), a connecting outer pipe (15) is arranged at the bottom of the receiving tray (14), a first filter screen (16) is arranged in the receiving tray (14), and a liquid containing tank (17) is arranged at one end of the crushing box (1);
separating mechanism is including setting up at inside churn (18) of separator box (3), churn (18) bottom is provided with rotor plate (19), rotor plate (19) bottom is provided with bull stick (20), bull stick (20) bottom is provided with second driving motor (21), churn (18) inside is provided with connecting pipe (22), connecting pipe (22) one end is provided with pump machine (23), pump machine (23) one end is provided with handles case (24), pump machine (23) output is provided with transmission pipe (25), it is provided with second filter screen (26) to handle case (24) inside, it is provided with fluid-discharge tube (27) to be located second filter screen (26) bottom inside handling case (24).
2. The apparatus of claim 1, wherein the apparatus comprises: broken case (1) top is provided with the blowing fill, baffle (4) slope sets up at broken case (1) inside wall, flourishing fluid reservoir (17) top is provided with the blowing fill, flourishing fluid reservoir (17) inside is provided with conveying pipe (28), conveying pipe (28) one end extends to inside broken case (1), conveying pipe (28) outside is provided with first connecting valve.
3. The apparatus of claim 1, wherein the apparatus comprises: first scraper blade (7) and second scraper blade (13) cross sectional shape all set up to trapezoidal, head rod (5) and second connecting rod (11) are the parallel direction setting, pivot (8) one end extends to the output of first driving motor (10), first driving motor (10) can be dismantled with broken case (1) lateral wall and be connected.
4. The apparatus of claim 1, wherein the apparatus comprises: conveyer pipe (2) one end extends to inside connecting outer tube (15), inside conveyer pipe (2) other end extends to churn (18), churn (18) and rotor plate (19) fixed connection.
5. The apparatus of claim 1, wherein the apparatus comprises: one end of the rotating rod (20) extends to the output end of the second driving motor (21), and one end of the connecting pipe (22) is detachably connected with the input end of the pump (23).
6. The apparatus of claim 1, wherein the apparatus comprises: the inside of the bearing tray (14) is provided with a sealing gasket at the bottom of the first filter screen (16), and the sealing gasket is made of rubber.
7. The apparatus of claim 1, wherein the apparatus comprises: one end of the transmission pipe (25) extends into the treatment box (24), a liquid inlet hole (29) is formed in the top of the treatment box (24), the liquid discharge pipe (27) extends to the bottom of the separation box (3), and a second connecting valve is arranged outside the liquid discharge pipe (27).
CN202022380146.7U 2020-10-23 2020-10-23 Gene sequencing instrument sample pretreatment device Expired - Fee Related CN213680653U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202022380146.7U CN213680653U (en) 2020-10-23 2020-10-23 Gene sequencing instrument sample pretreatment device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202022380146.7U CN213680653U (en) 2020-10-23 2020-10-23 Gene sequencing instrument sample pretreatment device

Publications (1)

Publication Number Publication Date
CN213680653U true CN213680653U (en) 2021-07-13

Family

ID=76759376

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202022380146.7U Expired - Fee Related CN213680653U (en) 2020-10-23 2020-10-23 Gene sequencing instrument sample pretreatment device

Country Status (1)

Country Link
CN (1) CN213680653U (en)

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CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20210713

CF01 Termination of patent right due to non-payment of annual fee