CN107581615A - Special diet containing Structure grease - Google Patents

Special diet containing Structure grease Download PDF

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Publication number
CN107581615A
CN107581615A CN201610532335.7A CN201610532335A CN107581615A CN 107581615 A CN107581615 A CN 107581615A CN 201610532335 A CN201610532335 A CN 201610532335A CN 107581615 A CN107581615 A CN 107581615A
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China
Prior art keywords
content
acid
structure grease
grease
palmitic acid
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CN201610532335.7A
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Chinese (zh)
Inventor
汪志明
李翔宇
陆姝欢
周强
汪锋
肖敏
易德伟
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Limited By Share Ltd Biotechnology (wuhan) Co Ltd
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Limited By Share Ltd Biotechnology (wuhan) Co Ltd
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Priority to CN201610532335.7A priority Critical patent/CN107581615A/en
Publication of CN107581615A publication Critical patent/CN107581615A/en
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Abstract

The present invention relates to a kind of Structure grease, and it is as obtained by vegetable oil is Material synthesis, and 2 palmitic acid contents of Sn are not less than 70%.The Structure grease of the present invention derives from vegetable oil, meets alimentary codex requirement, applied widely.Further, the content of 2 palmitic acids of Sn is not less than 70% in Structure grease, and Sn 1 is included in Structure grease, 3 are triglycerides that unrighted acid Sn 2 is palmitic acid, the ratio that the triglycerides accounts for total triglycerides is not less than 60%, it is sufficiently close to the trophic structure of breast milk, therefore the more conducively absorption of human body.

Description

Special diet containing Structure grease
Technical field
The present invention relates to a kind of special diet containing Structure grease.
Background technology
Triglycerides forms for the main grease of human consumption, and modern nutriology has confirmed that human body is in devils brew three During ester, can selective hydrolysis Sn-1, the aliphatic acid on 3, form Sn-2 monoglycerides and substantial amounts of free fatty.These Two kinds of reactions occur in free fatty in vivo:1st, by own absorption;2nd, with being discharged after other internal substance reactions.With trip Compared from unrighted acid, saturated fatty acid absorption rate of dissociating is slow, it is easier to is reacted with calcium, forms insoluble soap Calcium, and then be excreted.Accordingly, with respect to the triglycerides for producing free saturated fatty acid, free unsaturated fat is produced The triglycerides of acid is more conducive to the absorption of human body.
In breast milk, 98% or so fat exists in the form of triglycerides, wherein palmitic acid(C16:0, saturated fat Acid)Content account for 20-25%, oleic acid(C18:1, unrighted acid)Content accounts for 30-38%.Most palmitic acid distribution In the Sn-2 positions of triglycerides, and oleic acid is then mainly distributed on Sn-1 and Sn-3 positions, this unique triglycerides in breast milk Structure is referred to as 2- tripalmitins.At present, the component content of the baby formula milk powder of in the market and breast milk relatively, But substantial amounts of human trial and zoopery have confirmed that, the absorption of human milk fat is better than baby formula milk powder, wherein one Individual major reason is that this unique triglyceride structure in breast milk.This unique triglyceride structure can not only be protected The absorption of infant nutrient is demonstrate,proved, it digests principle and is equally applicable to some functions of intestines and stomach weakling groups, can make special diet Mitigate its stomach burden, while ensure enough nutrition.
Inspired by this unique triglyceride structure in breast milk, there is part manufacturer to utilize to natural oil at present The Structure grease of breast milk is simulated in the modification of fat to produce, and is added in baby milk powder and special diet, and this not only may be used To improve absorption efficiency of the human body to fat and calcium, moreover it is possible to reduce the hardness of excrement, reduce the probability of happening of constipation and intestinal obstruction. The characteristics of this Structure grease, is:Aliphatic acid on Sn-2 positions is mainly palmitic acid, Sn-1, and the aliphatic acid on 3 is mainly Unrighted acid(Unsaturated fattyacid, UFA).
China Patent Publication No. is CN102757988A application for a patent for invention, discloses the oleic acid -2- of 1,3- of one kind bis- palm fibres The preparation method of palmitic acid acid glycerol three ester, including:Glycerine and palmitic acid are subjected to esterification under catalyst action, three palm fibres are made Palmitic acid acid glyceride, then tripalmitin and oleic acid or oleate are acted on into lower progress ester exchange in 1,3 specific lipase Reaction, so as to obtain the OPO of high-purity.In this Structure grease, Sn-2 positions palmitic acid Content can reach 90%, however, the Structure grease of this high Sn-2 positions palmitic acid content, is closed by glycerine and palmitic acid Into can produce substantial amounts of soda acid discarded object in building-up process, be unfavorable for environmental protection.On the other hand, this method is obtained ties Structure grease does not meet the requirement of existing food relevant laws and regulations, and the scope of application can be extremely restricted.
Chinese Publication No. CN102776077A application for a patent for invention, disclose a kind of preparation of breast milk Structure grease Method, including palm oil of the palmitic acid content more than 50% is subjected to intramolecular rearrangement reaction under catalyst action, Sn- is made 2 palmitic acid contents are more than 50% triglycerides;By triglycerides of the Sn-2 positions palmitic acid content more than 50% with mixing fat Fat acid or its nonglyceride carry out ester exchange reaction under 1,3 specific lipase effect, and reaction product is post-treated to be obtained Breast milk Structure grease.However, preparation technology is limited to, and by this method in obtained Structure grease, Sn-2 positions palm A certain distance also be present with the composition proportion of breast milk, therefore nutritive value needs further typically 50% or so in acid content Lifting.
Therefore, it is necessary to develop a kind of more excellent technique, Sn-2 positions palmitic acid content in Structure grease can be improved, is carried simultaneously High Sn-1,3 positions be unrighted acid and Sn-2 positions be palmitic acid triglycerides content.The structure oil as made from the technique Fat, both closer with breast milk composition, beneficial to absorption of human body, and can meets food security and laws and regulations requirement.
The content of the invention
It is an object of the invention to provide a kind of nutritive value height and contain the special of Structure grease beneficial to absorption of human body Meals.
To realize above-mentioned main purpose, the present invention provides a kind of special diet containing Structure grease, in the Structure grease Sn-2 positions palmitic acid content is not less than 70%.
Further, in above-mentioned special diet, Structure grease is as obtained by vegetable oil is Material synthesis.
Further, Sn-1 is included in the Structure grease of above-mentioned special diet, 3 are unrighted acid and Sn-2 positions are The triglycerides of palmitic acid, the ratio that the triglycerides accounts for total triglycerides are not less than 60%, preferably 60%-80%.
Further, in above-mentioned special diet, unrighted acid is oleic acid, leukotrienes, arachidonic acid, 22 carbon Acid or eicosapentaenoic acid.
The present invention has the advantages that:First, Structure grease derives from vegetable oil, meets food security and regulation will Ask, can be added as the dispensing of the special diets such as infant;Further, the Sn-2 positions palmitic acid of this Structure grease Content is not less than 70%, and Sn-1 is included in Structure grease, 3 be unrighted acid and Sn-2 positions be palmitic acid glycerine three Ester, the ratio that the triglycerides accounts for total triglycerides are not less than 60%, and the structure of this and breast milk is sufficiently close to, therefore nutritive value It is higher, it is more beneficial for the absorption of human body.
Embodiment
With reference to instantiation, the Structure grease and special diet of the present invention are described in further detail.It is following In embodiment, all percentages are mass percent.
Embodiment 1
Preparation structure grease
(1) palm oil stearin is provided, the ratio that its palmitic acid accounts for total fatty acids is for 76.5%, Sn-2 positions palmitic acid content 60.6%, Sn-1,3 are oleic acid and Sn-2 positions are that the content of triglyceride of palmitic acid is 3.3%.Wherein, " Sn-2 positions palmitic acid contains Amount " refers to that palmitic acid on Sn-2 positions accounts for the content of all aliphatic acid on Sn-2 positions, and following embodiments use identical concept, after It is continuous to repeat no more.
(2) palm oil stearin 500g, potassium palmitate 250g and water 2.5g are placed in flask, stirring is warming up to 160 DEG C And keeping constant, for sustained response after 10 hours, centrifugal filtration removes soap, obtains fat A.
(3) take fat A 400g to be mixed with oleic acid 400g, be placed in flask, stirring is warming up to 60 DEG C and keeps constant, adds Enter hexane 10g and Sn-1,3 specific lipase 80g, react 8 hours(Ester exchange is oriented, similarly hereinafter), obtain miscella B.
(4) miscella B is refined, refined step includes but is not limited to:Lipase is filtered to remove, dry fractionation removes Free fatty, Structure grease is obtained after deodorization, the Structure grease is liquid at 25 DEG C.Pass through GC(High temperature capillary post, Fid detector, similarly hereinafter)And thin-layer chromatography detects, Sn-2 positions palmitic acid content is that 75.8%, Sn-1,3 are in the Structure grease Oleic acid and Sn-2 positions are that to account for the ratio of total triglycerides be 60.6% to the triglycerides of palmitic acid.
Prepare special diet
It is used to prepare a kind of special diet, i.e. babies ' formula milk powder using Structure grease obtained above.
(1) following composition and the raw material of ratio are provided:
Structure grease, content 20%;
Vegetable oil, content 6%;
Lactose, content 37%;
WPC, content 18%;
Skimmed milk power, content 15%;
FOS, content 1%;
Galactooligosaccharide, content 1%;
Mineral substance premix, content 1%;
Arachidonic acid oil, content 0.5%;
Docosahexaenoic acid grease, content 0.4%;
Vitamin premix, content 0.1%.
(2) feed liquid of solid concentration 52% is prepared according to above-mentioned formula rate, after 8000rpm rotating speed cuttings 30min, Homogeneous is carried out under 20MPa, obtains emulsion.This emulsion is subjected to press spray drying, spray drying parameters are as follows:
EAT:220℃
Leaving air temp:95℃
Charging rate:450g/min
Intake volume:3500m3/h
Atomisation pressure:22MPa
The babies ' formula milk powder that Structure grease content is 7% may finally be made in operation more than.
Embodiment 2
Preparation structure grease
(1) palm oil stearin is provided, its palmitic acid accounts for total fatty acids ratio and is for 76.5%, Sn-2 positions palmitic acid content 61.3%, Sn-1,3 are leukotrienes and Sn-2 positions are that the content of triglyceride of palmitic acid is 0.2%.
(2) palm oil stearin 2000g, sodium palmitate 2000g and water 10g are placed in flask, stirring is warming up to 160 DEG C and keep constant, sustained response, except soap, obtains fat A after 12 hours.
(3) take fat A 1000g to be mixed with leukotrienes 3000g, be placed in flask, stirring is warming up to 60 DEG C and keeps permanent It is fixed, hexamethylene 30g and Sn-1 are added, 3 specific lipase 200g, is reacted 10 hours(Ester exchange is oriented, similarly hereinafter), mixed Oily B.
(4) miscella B is refined, refined step includes but is not limited to:Lipase is filtered to remove, dry fractionation removes Free fatty, Structure grease is obtained after deodorization, the Structure grease is liquid at 25 DEG C.Pass through GC(High temperature capillary post, Fid detector)And thin-layer chromatography detects, Sn-2 positions palmitic acid content is that 81.6%, Sn-1,3 are leukotrienes in the Structure grease And Sn-2 positions are that to account for the ratio of total triglycerides be 64.3% for the triglycerides of palmitic acid.
Prepare special diet
It is used to prepare a kind of special diet, i.e. infant formula ground rice using Structure grease obtained above.Its formula is as follows:
(1) following composition and the raw material of ratio are provided:
Structure grease, content 6%;
Selected rice, content 89.9%;
White granulated sugar, content 3%;
Vitamin premix, content 0.1%;
Mineral substance premix, content 1%.
(2) rice is crushed, obtains the ground rice of about 80-100 mesh.According to above-mentioned formula, the material of 45% solid content of preparation Starch, after 6000rpm rotating speed cuttings 25min, homogeneous is carried out under 22MPa, obtains the slurry after homogeneous.Then 0.5% shallow lake is added Powder enzyme hydrolysis 4hr, is then gelatinized 30min at 80 DEG C.The slurry being gelatinized carries out steam roller tube drying according to following parameter:
Steam pressure:0.45MPa
Drum rotation speed:4rpm
Film thickness:3mm
Final obtain is operated more than can arrive moisture 3.0%, and Structure grease content is 2% infant formula ground rice.
Embodiment 3
Preparation structure grease
(1) palm oil stearin is provided, its palmitic acid accounts for total fatty acids ratio and is for 79.0%, Sn-2 positions palmitic acid content 66.0%, Sn-1,3 are arachidonic acid and Sn-2 positions are that the content of triglyceride of palmitic acid is 0%.
(2) palm oil stearin 7500g, methyl hexadecanoate 15000g, lipase 750g are placed in container, stirring rises Temperature is to 65 DEG C and keeps constant, and for sustained response after 12 hours, dry fractionation removes fatty acid methyl ester, obtains fat A.
(3) take fat A 5000g to mix with arachidonic acid 20000g to be placed in container, stirring is warming up to 70 DEG C and protected Hold constant, add octane 200g and Sn-1,3 specific lipase 2000g, react 8 hours(Ester exchange is oriented, similarly hereinafter), obtain Miscella B.
(4) miscella B is refined, refined step includes but is not limited to:Lipase is filtered to remove, dry fractionation removes Free fatty, Structure grease is obtained after deodorization, the Structure grease is liquid at 25 DEG C.Pass through GC(High temperature capillary post, Fid detector)And thin-layer chromatography detects, Sn-2 positions palmitic acid content is that 85.5%, Sn-1,3 are peanut four in the Structure grease Olefin(e) acid and Sn-2 positions are that to account for the ratio of total triglycerides be 75.0% to the triglycerides of palmitic acid.
Prepare special diet
It is used to prepare a kind of special diet using Structure grease obtained above, i.e. infant is relieved inflammation or internal heat treasured.
(1) following composition and the raw material of ratio are provided:
Structure grease, content 32%;
Casein sodium, content 4%;
Glucose syrup, content 35%;
Maltodextrin, content 25%;
Sodium ascorbate, content 4%.
(2) feed liquid of 50% solid concentration is prepared according to above-mentioned formula rate, after 9000rpm shears 30min, Homogeneous is carried out under 40MPa, obtains emulsion.This emulsion is subjected to centrifugal spray drying, spray drying parameters are as follows:
EAT:210℃
Leaving air temp:80℃
Charging rate:400g/min
Intake volume:3500m3/h
Rotary speed:4000rpm
Operation finally gives the microcapsule product that Structure grease content is 12% more than.
(3) above-mentioned microcapsule product is further used, is matched according to the following formulation:
Microcapsule product, content 19%;
Chrysanthemum powder, content 34%;
Honeysuckle powder, content 25%;
Lotus nut powder, content 15%;
Hawthorn powder, content 4.5%;
Mineral matter, content 1%;
Vitamin, content 1%;
Docosahexaenoic acid pulvis, content 0.5%;
(4) above-mentioned material is mixed into 35min with 18rpm speed in flat mixer, finally gives structure fat content Relieved inflammation or internal heat treasured for 2.3% infant.
Embodiment 4
Preparation structure grease
(1) a kind of palm oil stearin is provided, its palmitic acid accounts for total fatty acids ratio and is for 79.0%, Sn-2 positions palmitic acid content 66.0%, Sn-1,3 are docosahexaenoic acid and Sn-2 positions are that the content of triglyceride of palmitic acid is 0%.
(2) palm oil stearin 20000g, ethyl palmitate 80000g are pumped into and are filled with the continuous anti-of lipase 4000g Ying Zhuzhong, it is warming up to 70 DEG C and keeps constant, for sustained response after 15 hours, molecular distillation removes fatty-acid ethyl ester, obtains grease A。
(3) take fat A 10000g to mix with docosahexaenoic acid 60000g to be placed in container, stirring is warming up to 65 DEG C And keep constant, it is pumped into the successive reaction post for being filled with lipase 4000g, coutroi velocity, reacts 12 hours(Orient ester exchange, Similarly hereinafter), obtain miscella B.
(4) miscella B is refined, refined step includes but is not limited to:Dry fractionation removes free fatty, deodorization Structure grease is obtained afterwards, the Structure grease is liquid at 25 DEG C.Pass through GC(High temperature capillary post, fid detector, similarly hereinafter) And thin-layer chromatography detection, in the Structure grease Sn-2 positions palmitic acid content be 87.4%, Sn-1,3 be docosahexaenoic acid and Sn-2 positions are that the ratio that the triglycerides of palmitic acid accounts for total triglycerides is 80.9%.
Prepare special diet
It is used to prepare a kind of special diet, i.e. milk for children using Structure grease obtained above.
(1) following composition and the raw material of ratio are provided:
Plain chocolate, content 89.56%;
Arachidonic acid oil, content 0.02%;
Structure grease, content 3%;
White granulated sugar, content 6%;
Stearoyl lactate, content 0.1%;
Sodium alginate, content 0.1%;
Xanthans, content 0.1%;
Carragheen, content 0.1%;
Vitamin premix, content 0.01%;
Mineral substance premix, content 0.01%.
(2) above dispensing is processed according to following technique:
Milk is heated to 72 DEG C, stirring adds sugar and stabilizer, stirs 25min, is cooled to less than 40 DEG C;
Arachidonic acid oil and vitamin, mineral matter etc. are dissolved in advance with normal temperature milk, then adds and walks in milk, stir Mix uniformly;
Constant volume;
Homogeneous (62 DEG C, 22MPa);
UHT sterilizes (137 DEG C/4s);
It is filling.
Operation may finally obtain the milk for children that Structure grease content is 1.2% more than.
Embodiment 5
Preparation structure grease
(1) a kind of shortening is provided, it is that 53.8%, Sn-2 positions palmitic acid content is 42.5% that its palmitic acid, which accounts for total fatty acids ratio, Sn-1,3 positions are eicosapentaenoic acid and Sn-2 positions are that the content of triglyceride of palmitic acid is 0.
(2) palm oil stearin 480g, sodium palmitate 960g and water 4g are placed in container, stirring is warming up to 190 DEG C simultaneously Keep constant, sustained response adds acetone 600ml to be centrifuged off sodium palmitate after fully dissolving, obtain fat A after 10 hours.
(3) take fat A 250g to mix with eicosapentaenoic acid 750g to be placed in container, stirring is warming up to 45 DEG C and kept It is constant, isooctane 10g and Sn-1 are added, 3 specific lipase 50g, is reacted 8 hours(Ester exchange is oriented, similarly hereinafter), mixed Oily B.
(4) miscella B is refined, refined step includes but is not limited to:Lipase is filtered to remove, dry fractionation removes Free fatty, Structure grease is obtained after deodorization, the Structure grease is liquid at 25 DEG C.Pass through GC(High temperature capillary post, Fid detector, similarly hereinafter)And thin-layer chromatography detects, Sn-2 positions palmitic acid content is that 83.4%, Sn-1,3 are in the Structure grease Eicosapentaenoic acid and Sn-2 positions are that to account for the ratio of total triglycerides be 62.8% to the triglycerides of palmitic acid.
Prepare special diet
It is used to prepare a kind of special diet, i.e. person in middle and old age's Yoghourt using Structure grease obtained above.Its formula is as follows:
(1) following composition and the raw material of ratio are provided:
Plain chocolate, content 90.45%;
Docosahexaenoic acid grease, content 0.03%;
Structure grease, content 4.2%;
White granulated sugar, content 5%;
Agar, content 0.1%;
Gelatin, content 0.1%;
Carragheen, content 0.1%;
Vitamin premix, content 0.01%;
Mineral substance premix, content 0.01%.
(2) above dispensing is processed according to following technique:
Milk is heated to 65 DEG C, stirring adds sugar and stabilizer, stirs 20min, is cooled to less than 40 DEG C;
Docosahexaenoic acid grease, vitamin and mineral etc. are dissolved in advance with normal temperature milk, then adds and walks milk In, stir;
Constant volume;
Homogeneous (65 DEG C, 20MPa);
Pasteurization(95 DEG C, 5min);
43 DEG C during cooling, fermented bacterium is added, is fermented to pH4.6;
Stirring demulsification;
It is filling.
Operation may finally obtain person in middle and old age's Yoghourt that Structure grease content is 1.6% more than.

Claims (5)

1. the special diet containing Structure grease, it is characterised in that:Sn-2 positions palmitic acid content is not less than in the Structure grease 70%。
2. the special diet according to claim 1 containing Structure grease, it is characterised in that:The Structure grease is by plant Oil is obtained by Material synthesis.
3. the special diet according to claim 1 or 2 containing Structure grease, it is characterised in that:In the Structure grease Comprising Sn-1,3 be unrighted acid and Sn-2 positions be palmitic acid triglycerides, the triglycerides accounts for total triglycerides Ratio is not less than 60%, preferably 60%-80%.
4. the special diet according to claim 3 containing Structure grease, it is characterised in that:The unrighted acid is Oleic acid, leukotrienes, arachidonic acid, docosahexaenoic acid or eicosapentaenoic acid.
5. the special diet according to claim 4 containing Structure grease, it is characterised in that:The Structure grease by with Obtained by lower method:
Glyceride containing palmitic acid residues is provided;
Glyceride containing palmitic acid residues is mixed with palmitate or palmitic non-glyceride esters and carries out ester exchange, obtains grease A;
Fat A and unrighted acid are subjected to Sn-1,3 enzymatics orient ester exchange, obtain miscella B;
Miscella B is refined, Structure grease is made.
CN201610532335.7A 2016-07-08 2016-07-08 Special diet containing Structure grease Withdrawn CN107581615A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108865445A (en) * 2018-07-04 2018-11-23 嘉必优生物技术(武汉)股份有限公司 A method of reducing glyceride iodine number
WO2021104164A1 (en) * 2019-11-26 2021-06-03 瞿瀚鹏 Mortierella alpina and use thereof, and microbial oil rich in ara at position sn-2, preparation method therefor and use thereof
WO2022045951A1 (en) * 2020-08-31 2022-03-03 Aak Ab (Publ) A process for making a vegetable fat compositions with an increased amount of sn2 positioned palmitic acid
WO2022045953A1 (en) * 2020-08-31 2022-03-03 Aak Ab (Publ) A process for production of a vegetable oil composition having at least 50% palmitic acid present in the sn2-position out of total palmitic acid in the triglycerides of the vegetable oil composition

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102776077A (en) * 2012-08-07 2012-11-14 浙江大学 Preparation method of grease with humanized structure
CN102827885A (en) * 2011-06-17 2012-12-19 丰益(上海)生物技术研发中心有限公司 Composition containing 1,3-di-unsaturated fatty acyl-2-saturated fatty acyl glyceryl ester as well as preparation method and application thereof
CN104087626A (en) * 2014-07-07 2014-10-08 嘉必优生物工程(武汉)有限公司 Method for preparing composition containing 2-palmitin
CN105112464A (en) * 2015-08-19 2015-12-02 嘉必优生物工程(武汉)有限公司 Structural fat and special diet containing same

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102827885A (en) * 2011-06-17 2012-12-19 丰益(上海)生物技术研发中心有限公司 Composition containing 1,3-di-unsaturated fatty acyl-2-saturated fatty acyl glyceryl ester as well as preparation method and application thereof
CN102776077A (en) * 2012-08-07 2012-11-14 浙江大学 Preparation method of grease with humanized structure
CN104087626A (en) * 2014-07-07 2014-10-08 嘉必优生物工程(武汉)有限公司 Method for preparing composition containing 2-palmitin
CN105112464A (en) * 2015-08-19 2015-12-02 嘉必优生物工程(武汉)有限公司 Structural fat and special diet containing same

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108865445A (en) * 2018-07-04 2018-11-23 嘉必优生物技术(武汉)股份有限公司 A method of reducing glyceride iodine number
WO2021104164A1 (en) * 2019-11-26 2021-06-03 瞿瀚鹏 Mortierella alpina and use thereof, and microbial oil rich in ara at position sn-2, preparation method therefor and use thereof
WO2022045951A1 (en) * 2020-08-31 2022-03-03 Aak Ab (Publ) A process for making a vegetable fat compositions with an increased amount of sn2 positioned palmitic acid
WO2022045953A1 (en) * 2020-08-31 2022-03-03 Aak Ab (Publ) A process for production of a vegetable oil composition having at least 50% palmitic acid present in the sn2-position out of total palmitic acid in the triglycerides of the vegetable oil composition

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Application publication date: 20180116