CN107581253A - A kind of Zinc-rich saccharomyces cerevisiae solid beverage and preparation method thereof - Google Patents
A kind of Zinc-rich saccharomyces cerevisiae solid beverage and preparation method thereof Download PDFInfo
- Publication number
- CN107581253A CN107581253A CN201710774198.2A CN201710774198A CN107581253A CN 107581253 A CN107581253 A CN 107581253A CN 201710774198 A CN201710774198 A CN 201710774198A CN 107581253 A CN107581253 A CN 107581253A
- Authority
- CN
- China
- Prior art keywords
- parts
- zinc
- saccharomyces cerevisiae
- rich saccharomyces
- solid beverage
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention belongs to food technology field, and in particular to a kind of Zinc-rich saccharomyces cerevisiae solid beverage and preparation method thereof.Zinc-rich saccharomyces cerevisiae solid beverage of the present invention includes following components and its parts by weight：24 parts of 36 parts of Zinc-rich saccharomyces cerevisiae, 48 parts of Sweet tea leaf extract, 10 15 parts of milk powder, 36 parts of oligoisomaltose, 28 parts of carboxymethyl chitosan, 48 parts of xylo-oligosaccharide, 10 20 parts of tapioca starch, 24 parts of flavouring, 25 parts of sweet potato pectin and anticaking agent.The technical advantages of the present invention are that：The characteristics of solid beverage of the present invention has good dispersiveness, mobility, reconstitutes effect and be aesthetic good；The addition of Zinc-rich saccharomyces cerevisiae causes product to have good zinc supplementation effect；The component such as Zinc-rich saccharomyces cerevisiae, the Sweet tea leaf extract and sweet potato pectin and carboxymethyl chitosan synergy that cooperates has fat reducing and weight reducing function.
Description
Technical field
The invention belongs to food technology field, and in particular to a kind of Zinc-rich saccharomyces cerevisiae solid beverage and preparation method thereof.
Background technology
Solid beverage is to add appropriate auxiliary material or food addition with sugar, newborn and dairy products, egg or egg products etc. for raw material
Solid articles made of agent, have the characteristics that easy to carry, raciness and instant capacity are good.
Zinc is that the essential trace element of human body only has 2-3g in human body, but accounts for 15-20% in skin, helps cell
Division, during animal zinc-deficiency except maldevelopment, Reproductive Performance is low and skeletal abnormality in addition to, it also occur that the lesion such as depilation.Other zinc
It is the vigorous and graceful indispensable traditional Chinese medicine ingredients of skin, the synthesis to fibroblastic hyperplasia and collagenous fibres is all particularly important.
The zinc in the dermatosis patient serum such as psoriasis, seborrhea, acne vulgaris is below the level of Healthy People according to surveying and determination.
Chinese patent application CN104872755A discloses a kind of zinc supplementing drink, its prepared by following raw material and
Into：Inspissated juice 22-30 parts, zinc gluconate 2-4 parts, white granulated sugar 4-8 parts, citric acid 0.3-0.5 parts, stabilizer 0.1-0.3
Part, sweetener 0.3-0.5 parts, essence 0.1-0.3 parts, drinking water 160-200 parts.Moderate also have though solid beverage of the present invention is sour-sweet
The effect of zinc supplementation, but mouthfeel is enriched by increasing essence, do not meet the expectation of consumer.
Saccharomycete itself is nutritious rich in health factors such as dietary fibers, and gold is added during culture yeasts
Belong to element and absorption and conversion by yeast in growth course to metallic element, metal and the albumen in yeast can be made
Matter and polysaccharide organic combine, and Chinese patent CN101361567B discloses a kind of multi-nutrilite composite yeast powder, including by matter
The 45-60% of gauge high-protein yeast, 10-30% Zinc-rich saccharomyces cerevisiae, 10-30% ferrum-rich saccharomyces cerevisiae, 1-5% selenium-rich ferment
Other dispensings of female, 1-5% Cr-enriched yeast and surplus, wherein the protein content of the high-protein yeast is 50-60%.
It is used as raw-food material, although can be that human body supplements the elements such as selenium, zinc and chromium needed by human, its nutritional ingredient master
Saccharomycete is come from, and other nutrient composition contents are less, it is impossible to it is balanced comprehensively to reach nutrition.
The content of the invention
To solve problems of the prior art, the present invention provides a kind of Zinc-rich saccharomyces cerevisiae solid beverage and its preparation side
Method.
A kind of Zinc-rich saccharomyces cerevisiae solid beverage, including following components and its parts by weight：
Zinc-rich saccharomyces cerevisiae 3-6 parts, Sweet tea leaf extract 4-8 parts, milk powder 10-15 parts, oligoisomaltose 3-6 parts, carboxymethyl
Chitosan 2-8 parts, xylo-oligosaccharide 4-8 parts, tapioca starch 10-20 parts, flavouring 2-4 parts, sweet potato pectin 2-5 parts and anticaking agent 2-4
Part.
Preferably, described Zinc-rich saccharomyces cerevisiae solid beverage, is made up of following components and its parts by weight：
4.5 parts of Zinc-rich saccharomyces cerevisiae, 6 parts of Sweet tea leaf extract, 12.5 parts of milk powder, 4.5 parts of oligoisomaltose, carboxymethyl chitosan
3 parts of 5 parts of sugar, 6 parts of xylo-oligosaccharide, 15 parts of tapioca starch, 3 parts of flavouring, 3.5 parts of sweet potato pectin and anticaking agent.
Preferably, the preparation method of described Sweet tea leaf extract comprises the following steps：
1) take sweet tea to be crushed to 80 mesh, be put into the ethanol water that volume fraction is 60%, surpass in 45 DEG C, 40KHz
Refluxing extraction 3 times under sound wave, 15min is extracted every time, merge extract solution filtering and concentrating, obtain Sweet tea Leave extract；
2) add sodium carbonate to adjust pH to 8.8 in Sweet tea Leave extract obtained by step 1), filter to take supernatant；
3) supernatant obtained by step 2) is adjusted into pH to 7 with citric acid, adsorbing separation is carried out to it with D101 macroreticular resins, used
The ethanol solution that volume fraction is 80% elutes, and takes eluent to be concentrated and dried and produces.
Preferably, described milk powder is by bovine colostrum, skimmed milk powder and whole-fat milk powder in mass ratio 1:4:5 compositions.
Preferably, described flavouring is by glycyrrhizin and stevioside in mass ratio 2:3 compositions.
Preferably, the preparation method of described sweet potato pectin is：
(1) take sweet potato dregs 100g to add 3L water to stir, screening process 25min is carried out to it, collection can pass through aperture
For 175 μm of screen clothes but can not be by the part of 50 μm of screen clothes, dry Rhizoma Dioscoreae esculentae dietary fiber；
(2) 1L water is added into Rhizoma Dioscoreae esculentae dietary fiber obtained by step (1), lower addition 25g amylase is stirred, reacts 20min
After centrifuge 25min, it is standby to collect precipitation；
(3) Na that 200ml concentration is 0.8mol/L is added in being precipitated obtained by step (2)2HPO4Solution, it is anti-under stirring
Supernatant is collected by centrifugation after answering 25min, distillation and concentration is drying to obtain.
Preferably, the amylase described in step (2) is by alpha amylase and Pullulanase in mass ratio 2:3 compositions.
Further preferably, described anticaking agent is microcrystalline cellulose.
In addition, present invention also offers a kind of preparation method of Zinc-rich saccharomyces cerevisiae solid beverage, comprise the following steps：
S1, Zinc-rich saccharomyces cerevisiae, Sweet tea leaf extract, milk powder, oligoisomaltose, carboxymethyl chitosan are weighed in parts by weight
Sugar, xylo-oligosaccharide, tapioca starch, flavouring, sweet potato pectin and anticaking agent are well mixed, and are added in trough type mixing machine, are turned on the power
Water is added by the 5-15% of material total amount when agitating paddle rotates in machine to be mixed, lower reaction 15-35min is stirred after closing the lid, is obtained
Mixture；
S2, take 40 eye mesh screens to be installed in oscillating granulator, add mixture obtained by step S1, start granulator and carry out
Granulation；
S3, the step S2 particles made are sent to boiling drier, 45-55min is dried at 35-45 DEG C, is produced.
Oligoisomaltose CAS：499-40-1；Carboxymethyl chitosan CAS：83512-85-0；Glycyrrhizin CAS
Number：1405-86-3；Stevioside CAS：57817-89-7；Microcrystalline cellulose CAS：9004-34-6.
Yeast is rich in protein, mineral matter, vitamin and dietary fiber in itself, is a kind of very high-quality food source,
Making yeast by zymotechnique, Zn accumulation makes it be converted into biological form during the fermentation, reduces toxicity and stimulates increasing to human body
Add and absorb.Zinc-rich saccharomyces cerevisiae be during culture yeasts add Zn-ef ficiency and by yeast in growth course to zinc member
The absorption and conversion of element, make zinc be combined with the protein in yeast and polysaccharide organic, so as to eliminate the inorganic of chemical property
Zinc and organic zinc are stimulated the toxicity and stomach of human body, enable the more efficient utilization that is more safely absorbed by the body of zinc.
Sweet tea used in the present invention is one kind in rose family rubus plant, among the people to be used as medicine available for kidney tonifying decompression,
Glycosides containing more than 3.2% flavones, 8.94% sweet tea polyphenols and 4-5% in other sweet tea, have necessarily
Weight-reducing and lipid-lowering effect；Sweet potato pectin can promote the excretion of fecal fat, neutral steroids and bile, increase neutral steroids
Excretion, advantageously reduce the illness rate of the cancer relevant with sex hormone.
Compared with prior art, the technical advantages of the present invention are that：Solid beverage of the present invention has good dispersiveness, stream
The characteristics of moving property, reconstituting effect and be aesthetic good, and there is good zinc supplementation effect；Zinc-rich saccharomyces cerevisiae, Sweet tea leaf extract with
The component such as sweet potato pectin and carboxymethyl chitosan, which cooperates to act synergistically, has fat reducing and weight reducing function.
Embodiment
Below by specific embodiment, the present invention is described further, and these embodiments are only used for the purpose of illustration,
It is not intended to limit protection scope of the present invention.
Bovine colostrum is purchased from Guangzhou Hui Jian bio tech ltd；Skimmed milk powder is limited purchased from Shenzhen Hang Seng biotechnology
Company；Whole-fat milk powder is purchased from Sichuan Fu Shengde bio tech ltd；Xylo-oligosaccharide is limited purchased from the safe and sound biotechnology in Shenzhen
Company.
A kind of Zinc-rich saccharomyces cerevisiae solid beverage of embodiment 1
Described Zinc-rich saccharomyces cerevisiae solid beverage is made up of following components and its parts by weight：
It is 3 parts of Zinc-rich saccharomyces cerevisiae, 4 parts of Sweet tea leaf extract, 1 part of bovine colostrum, 4 parts of skimmed milk powder, 5 parts of whole-fat milk powder, oligomeric
3 parts of isomaltose, 2 parts of carboxymethyl chitosan, 4 parts of xylo-oligosaccharide, 10 parts of tapioca starch, 0.8 part of glycyrrhizin, 1.2 parts of stevioside,
2 parts of 2 parts of sweet potato pectin and microcrystalline cellulose.
The preparation method of described Sweet tea leaf extract comprises the following steps：
1) take sweet tea to be crushed to 80 mesh, be put into the ethanol water that volume fraction is 60%, surpass in 45 DEG C, 40KHz
Refluxing extraction 3 times under sound wave, 15min is extracted every time, merge extract solution filtering and concentrating, obtain Sweet tea Leave extract；
2) add sodium carbonate to adjust pH to 8.8 in Sweet tea Leave extract obtained by step 1), filter to take supernatant；
3) supernatant obtained by step 2) is adjusted into pH to 7 with citric acid, adsorbing separation is carried out to it with D101 macroreticular resins, used
The ethanol solution that volume fraction is 80% elutes, and takes eluent to be concentrated and dried and produces.
The preparation method of described sweet potato pectin is：
(1) take sweet potato dregs 100g to add 3L water to stir, screening process 25min is carried out to it, collection can pass through aperture
For 175 μm of screen clothes but can not be by the part of 50 μm of screen clothes, dry Rhizoma Dioscoreae esculentae dietary fiber；
(2) 1L water is added into Rhizoma Dioscoreae esculentae dietary fiber obtained by step (1), lower addition 25g amylase is stirred, reacts 20min
After centrifuge 25min, it is standby to collect precipitation；
(3) Na that 200ml concentration is 0.8mol/L is added in being precipitated obtained by step (2)2HPO4Solution, it is anti-under stirring
Supernatant is collected by centrifugation after answering 25min, distillation and concentration is drying to obtain.
The preparation method of described Zinc-rich saccharomyces cerevisiae solid beverage, comprises the following steps：
S1, Zinc-rich saccharomyces cerevisiae is weighed in parts by weight, be Sweet tea leaf extract, bovine colostrum, skimmed milk powder, whole-fat milk powder, low
IMO, carboxymethyl chitosan, xylo-oligosaccharide, tapioca starch, glycyrrhizin, stevioside, sweet potato pectin and microcrystalline cellulose
It is well mixed, add in trough type mixing machine, turn on the power and added when agitating paddle rotates in machine to be mixed by the 5% of material total amount
Water, lower reaction 15min is stirred after closing the lid, obtains mixture；
S2, take 40 eye mesh screens to be installed in oscillating granulator, add mixture obtained by step S1, start granulator and carry out
Granulation；
S3, the step S2 particles made are sent to boiling drier, 45min is dried at 35 DEG C, is produced.
A kind of Zinc-rich saccharomyces cerevisiae solid beverage of embodiment 2
Described Zinc-rich saccharomyces cerevisiae solid beverage is made up of following components and its parts by weight：
4.5 parts of Zinc-rich saccharomyces cerevisiae, 6 parts of Sweet tea leaf extract, 1.25 parts of bovine colostrum, 5 parts of skimmed milk powder, whole-fat milk powder 6.25
Part, 4.5 parts of oligoisomaltose, 5 parts of carboxymethyl chitosan, 6 parts of xylo-oligosaccharide, 15 parts of tapioca starch, 1.2 parts of glycyrrhizin, sweet tea
3 parts of 1.8 parts of synanthrin, 3.5 parts of sweet potato pectin and microcrystalline cellulose.
Described Sweet tea leaf extract and the preparation method of sweet potato pectin are similar to Example 1.
The preparation method of described Zinc-rich saccharomyces cerevisiae solid beverage, comprises the following steps：
S1, Zinc-rich saccharomyces cerevisiae is weighed in parts by weight, be Sweet tea leaf extract, bovine colostrum, skimmed milk powder, whole-fat milk powder, low
IMO, carboxymethyl chitosan, xylo-oligosaccharide, tapioca starch, glycyrrhizin, stevioside, sweet potato pectin and microcrystalline cellulose
It is well mixed, add in trough type mixing machine, turn on the power and added when agitating paddle rotates in machine to be mixed by the 10% of material total amount
Water, lower reaction 25min is stirred after closing the lid, obtains mixture；
S2, take 40 eye mesh screens to be installed in oscillating granulator, add mixture obtained by step S1, start granulator and carry out
Granulation；
S3, the step S2 particles made are sent to boiling drier, 50min is dried at 40 DEG C, is produced.
A kind of Zinc-rich saccharomyces cerevisiae solid beverage of embodiment 3
Described Zinc-rich saccharomyces cerevisiae solid beverage is made up of following components and its parts by weight：
6 parts of Zinc-rich saccharomyces cerevisiae, 8 parts of Sweet tea leaf extract, 1.5 parts of bovine colostrum, 6 parts of skimmed milk powder, 7.5 parts of whole-fat milk powder,
6 parts of oligoisomaltose, 8 parts of carboxymethyl chitosan, 8 parts of xylo-oligosaccharide, 20 parts of tapioca starch, 1.6 parts of glycyrrhizin, stevioside
4 parts of 2.4 parts, 5 parts of sweet potato pectin and microcrystalline cellulose.
Described Sweet tea leaf extract and the preparation method of sweet potato pectin are similar to Example 1.
The preparation method of described Zinc-rich saccharomyces cerevisiae solid beverage, comprises the following steps：
S1, Zinc-rich saccharomyces cerevisiae is weighed in parts by weight, be Sweet tea leaf extract, bovine colostrum, skimmed milk powder, whole-fat milk powder, low
IMO, carboxymethyl chitosan, xylo-oligosaccharide, tapioca starch, glycyrrhizin, stevioside, sweet potato pectin and microcrystalline cellulose
It is well mixed, add in trough type mixing machine, turn on the power and added when agitating paddle rotates in machine to be mixed by the 15% of material total amount
Water, lower reaction 35min is stirred after closing the lid, obtains mixture；
S2, take 40 eye mesh screens to be installed in oscillating granulator, add mixture obtained by step S1, start granulator and carry out
Granulation；
S3, the step S2 particles made are sent to boiling drier, 55min is dried at 45 DEG C, is produced.
A kind of Zinc-rich saccharomyces cerevisiae solid beverage of comparative example 1
Described Zinc-rich saccharomyces cerevisiae solid beverage is made up of following components and its parts by weight：
4.5 parts of zinc gluconate, 6 parts of Sweet tea leaf extract, 1.25 parts of bovine colostrum, 5 parts of skimmed milk powder, whole-fat milk powder
6.25 parts, 4.5 parts of oligoisomaltose, 5 parts of carboxymethyl chitosan, 6 parts of xylo-oligosaccharide, 15 parts of tapioca starch, glycyrrhizin 1.2
Part, 1.8 parts of stevioside, 3.5 parts of sweet potato pectin and 3 parts of microcrystalline cellulose.
The difference of comparative example 1 and embodiment 2 is：Zinc-rich saccharomyces cerevisiae is replaced with into zinc gluconate.
A kind of Zinc-rich saccharomyces cerevisiae solid beverage of comparative example 2
Described Zinc-rich saccharomyces cerevisiae solid beverage is made up of following components and its parts by weight：
4.5 parts of Zinc-rich saccharomyces cerevisiae, 6 parts of Semen phaseoli radiati extract, 1.25 parts of bovine colostrum, 5 parts of skimmed milk powder, whole-fat milk powder 6.25
Part, 4.5 parts of oligoisomaltose, 5 parts of carboxymethyl chitosan, 6 parts of xylo-oligosaccharide, 15 parts of tapioca starch, 1.2 parts of glycyrrhizin, sweet tea
3 parts of 1.8 parts of synanthrin, 3.5 parts of sweet potato pectin and microcrystalline cellulose.
The difference of comparative example 2 and embodiment 2 is：Sweet tea leaf extract is replaced with into Semen phaseoli radiati extract, its preparation method
It is similar with the preparation method of Sweet tea leaf extract.
A kind of Zinc-rich saccharomyces cerevisiae solid beverage of comparative example 3
Described Zinc-rich saccharomyces cerevisiae solid beverage is made up of following components and its parts by weight：
4.5 parts of Zinc-rich saccharomyces cerevisiae, 6 parts of Sweet tea leaf extract, 1.25 parts of bovine colostrum, 5 parts of skimmed milk powder, whole-fat milk powder 6.25
Part, 4.5 parts of oligoisomaltose, 5 parts of carboxymethyl chitosan, 6 parts of xylo-oligosaccharide, 15 parts of tapioca starch, 1.2 parts of glycyrrhizin, sweet tea
3 parts of 1.8 parts of synanthrin, 3.5 parts of chrysanthemum extract and microcrystalline cellulose.
The difference of comparative example 3 and embodiment 2 is：Sweet potato pectin will replace with chrysanthemum extract, its preparation method with it is sweet
The preparation method of potato pectin is similar.
Test example 1：Traditional performance determines
1. test material：Embodiment 1-3 and comparative example 1-3 solid beverage.
2. test method：
(1) albumen dispersion index PDI：3g Zinc-rich saccharomyces cerevisiae solid beverages are added in 100mL boiling water, are stirred well to molten
Solution, centrifugation take supernatant, and precipitation adds 50mL water, centrifuges and discard precipitation, supernatant is merged, determines its crude protein content again.
By formula：
Crude protein content in crude protein/dry-eye disease of PDI=supernatants, calculate.
(2) it is dispersed：3g Zinc-rich saccharomyces cerevisiae solid beverages are added in 25 DEG C of water, recorded since stirring to whole points
The time required to dissipating.
(3) mobility：Angle of repose injection method, 50g Zinc-rich saccharomyces cerevisiaes solid beverage is fallen into horizontal positioned put down along funnel
On plate, after solid beverage is fallen completely, the angle of solid beverage heap inclined-plane and flat board on flat board is tested, is denoted as angle of repose.
(4) bulk density：Zinc-rich saccharomyces cerevisiae solid beverage is scattered from funnel into 10mL graduated cylinders, measure 10mL solid drinks
The weight of material, bulk density is converted into, as a result as shown in table 1.
The performance test results of table 1
| Group | Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 | Comparative example 2 | Comparative example 3 |
| PDI/% | 96.13 | 98.32 | 94.60 | 88.57 | 80.70 | 81.61 |
| Dispersiveness/s | 40 | 35 | 45 | 50 | 60 | 55 |
| Mobility/° | 18 | 15 | 20 | 24 | 30 | 28 |
| Bulk density/gmL-1 | 0.5047 | 0.5648 | 0.4585 | 0.4009 | 0.3748 | 0.3736 |
As shown in Table 1, the Zinc-rich saccharomyces cerevisiae solid beverage that prepared by 1-3 of the embodiment of the present invention has compared with comparative example 1-3 solid beverages
Have more preferable dispersiveness, mobility, and more easily dissolve, illustrate the present invention using Zinc-rich saccharomyces cerevisiae, Sweet tea leaf extract and
The components such as carboxymethyl chitosan, which cooperate to act synergistically, causes prepared solid beverage to have preferably dispersed, flowing
Property, more easily dissolve.The albumen dispersion index value highest of additional embodiment 2, dispersiveness and mobility preferably and bulk density most
Height, it is the preferred embodiments of the present invention.
Test example 2：Reconstitute effect assessment
1. test material：Embodiment 1-3 and comparative example 1-3 solid beverage.
2. test method：
A1、B1、C1Group：The solid beverage 3g that Example 1-3 is prepared, 100mL boiling water is added, is stirred well to molten
Solution, the solid beverage sample that must have been reconstituted, and effect assessment is reconstituted to it.
A2、B2、C2Group：The solid beverage 3g that Example 1-3 is prepared, 60 DEG C of water of 100mL are added, are stirred well to
Dissolving, the solid beverage sample that must have been reconstituted, and effect assessment is reconstituted to it.
A3、B3、C3Group：The solid beverage 3g that Example 1-3 is prepared, 100mL normal-temperature waters are added, are stirred well to
Dissolving, the solid beverage sample that must have been reconstituted, and effect assessment is reconstituted to it.
D, E, F group：The solid beverage 3g that comparative example 1-3 is prepared is taken, 100mL boiling water is added, is stirred well to dissolving,
The solid beverage sample that must have been reconstituted, and effect assessment is reconstituted to it.
Table 2 reconstitutes effect assessment result
| Group | Reconstitute effect |
| A1、B1、C1Group | It can quickly stir, be rapidly reached the effect of half solidification, but still retain mobility, mouthfeel is oiliness, and sweet taste is full |
| D groups | Not yet stir and solidify, poor fluidity, mouthfeel is more greasy |
| E groups | Not yet stir and solidify, pleasantly sweet but not lasting, mouthfeel is thin |
| F groups | Can quickly it stir, mouthfeel is more greasy |
| A2-3、B2-3、C2-3Group | It can quickly stir, be rapidly reached the effect of half solidification, but still retain mobility, mouthfeel is oiliness, and sweet taste is full |
As shown in Table 2, the Zinc-rich saccharomyces cerevisiae solid beverage that prepared by 1-3 of the embodiment of the present invention reconstitutes effect compared with comparative example 1-3
Preferably, and to reconstitute water temperature unrestricted, illustrates the present invention using groups such as Zinc-rich saccharomyces cerevisiae, Sweet tea leaf extract and carboxymethyl chitosans
The synergy that point cooperates causes prepared solid beverage has preferably to reconstitute effect.
Test example 3：Zinc supplementation effect test
1. test material：Embodiment 1-3 and comparative example 1 solid beverage.
2. subjects：Male mice in kunming, quality 18-22g.
3. test method：
It is prepared by mouse zinc-deficiency feed：From it is cheap and containing the seldom rice protein powder of zinc as protein sources, golden dragonfish
Board salad oil is as fat source, and medical glucose is as sugar source, vitamin mixtures and the mineral salt mixture for being not added with zinc salt.
The foundation of mouse zinc-deficiency model：The actual measurement Zn content of zinc-deficiency feed is less than 2mg/kg, takes mouse to be grouped every group at random
10 feed in the cage of plastics laboratory, indoor environment relative humidity 55-65%, 22-24 DEG C of temperature.Fed through the 3d laundering period
Afterwards, zinc-deficiency group feeds low zinc feed, control group fed chow diet, free choice feeding and drinking water, observes mouse symptom, feeds
16d。
It is divided into control group normal mouse, zinc-deficiency group, zinc-deficiency+above-described embodiment 1-3 or the solid beverage test group of comparative example 1,
Every group of 10 feeding conditions are same as above, trial drug group gavage feeding, and feeding amount is 3ml/kg continuous gavages 16d.Test latter stage from
Eye socket takes blood system is standby from serum to survey；Put to death and cut open the belly immediately and take mouse liver, with 0.9% physiological saline rinse, remove blood with
Connective tissue, filter paper wipe it is dry, with 0.9% physiological saline using mass ratio as 1:9 are made homogenate.Determine serum and liver Zn content.
Mouse liver, Determination of Zinc Concentration in Serum change are as shown in table 3 after zinc supplementation.
The mouse liver of table 3 and Determination of Zinc Concentration in Serum change
| Detection project | Normal group | Zinc-deficiency group | Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 |
| Zn content μ g/g in liver | 36.64 | 3.28 | 32.39 | 36.27 | 31.39 | 26.37 |
| Determination of Zinc Concentration in Serum μ g/g | 6.72 | 3.48 | 6.07 | 6.69 | 5.84 | 4.26 |
From table 3 it can be seen that 1-3 of the embodiment of the present invention prepare Zinc-rich saccharomyces cerevisiae solid beverage compared with comparative example 1 for mouse
The incrementss of liver and Determination of Zinc Concentration in Serum are bigger, illustrate that the present invention coordinates Sweet tea leaf extract, methylol using Zinc-rich saccharomyces cerevisiae
The components such as chitosan coordinate compared with zinc gluconate and other components causes prepared solid beverage to have more preferable zinc supplementation effect.
And mouse liver and Determination of Zinc Concentration in Serum are the preferred embodiments of the present invention close to normal level after the zinc supplementation of embodiment 2.
Test example 4：Weight-reducing and lipid-lowering effect assessment
1. test material：Embodiment 1-3 and comparative example 2-3 solid beverage.
2. subjects：SD rats 100, body weight 70-90g males, purchased from Zhongshan University's Animal Experimental Study center.
3. test method：It is grouped at random after 100 rat adaptability are fed 1 week, 10 rats, which are given, maintains feed to make
For Normal group, 90 rats give high-calorie feed, and wherein high-calorie feed is by maintenance feed 66%, sucrose 15%, pig
Oil 15%, casein 3% and calcium monohydrogen phosphate 1% form.Record weekly to appetite, berley amount, surplus appetite and weigh in 1 time.
90 rats for giving high-calorie feed are sorted by weight gain after feeding 2 weeks, 30 relatively low obesities of weight gain is eliminated and supports
Anti- rat.Give filter out 60 fat Sensitive Rats to high-calorie feed again 5 weeks, Normal group is given maintenance and raised simultaneously
Material.
60 fat Sensitive Rats are randomly divided into 6 groups by the modeling phase after terminating by body weight, respectively model control group, implement
Example 1-3 groups and comparative example 2-3 groups every group 10.Embodiment 1-3 groups and comparative example 2-3 groups are filled daily by 10g/kg dosage respectively
Stomach gives the daily gavage of corresponding given the test agent once, and Normal group and model control group rat oral gavage give isometric steaming
Distilled water.
Each group rat continuously gives corresponding given the test agent 6 weeks, record weekly each group rat to appetite, surplus appetite and berley
Measure and weigh in 1 time.Normal group gives maintenance feed during experiment, and remaining each group gives high-calorie feed.Experiment knot
Weighed after beam, calculate the food ration that rat body weight incrementss calculate rat simultaneously, food ration and=to appetite-remain appetite-berley
Amount.All rats are injected intraperitoneally into 1% amobarbital by 0.5ml/100g dosage to be anaesthetized, solution is taken around testis and kidney
Fat is weighed as body fat and calculates fat/body ratio, as a result as shown in table 4.
Influence of 4 solid beverage of the present invention of table to obese rat food ration, body weight evolution and fat/body ratio
| Detection project | Normal group | Model control group | Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 2 | Comparative example 3 |
| Food-intake/g/d | 30.18 | 29.26 | 26.36 | 25.86 | 27.21 | 27.79 | 28.24 |
| Body weight evolution/g | 138.27 | 137.69 | 96.28 | 81.36 | 88.49 | 102.45 | 112.34 |
| Fat/body ratio/% | 2.48 | 4.64 | 3.36 | 2.83 | 3.28 | 4.12 | 4.09 |
From table 4, it can be seen that the rat appetite of solid beverage prepared by gavage embodiment of the present invention 1-3 and comparative example 2-3
Group is compared to having declined, and increased weight is slow, fat/body reduces than obvious, illustrates that the present invention uses Zinc-rich saccharomyces cerevisiae, sweet tea to extract
The component such as thing and oligoisomaltose and carboxymethyl chitosan, which cooperates to act synergistically, has fat reducing and weight reducing function, implements in addition
The increased weight of 2 groups of rats of example is the slowest, and fat/body is the most obvious than reducing, and is the preferred embodiments of the present invention.
The above is only the preferred embodiment of the present invention, it should be pointed out that be above-mentioned preferred embodiment be not construed as to this
The limitation of invention, protection scope of the present invention should be defined by claim limited range.For the general of the art
For logical technical staff, without departing from the spirit and scope of the present invention, some improvements and modifications can also be made, these improvement
Protection scope of the present invention is also should be regarded as with retouching.
Claims (9)
1. a kind of Zinc-rich saccharomyces cerevisiae solid beverage, it is characterised in that including following components and its parts by weight：
Zinc-rich saccharomyces cerevisiae 3-6 parts, Sweet tea leaf extract 4-8 parts, milk powder 10-15 parts, oligoisomaltose 3-6 parts, carboxymethyl chitosan
Sugared 2-8 parts, xylo-oligosaccharide 4-8 parts, tapioca starch 10-20 parts, flavouring 2-4 parts, sweet potato pectin 2-5 parts and anticaking agent 2-4 parts.
2. Zinc-rich saccharomyces cerevisiae solid beverage according to claim 1, it is characterised in that by following components and its parts by weight array
Into：4.5 parts of Zinc-rich saccharomyces cerevisiae, 6 parts of Sweet tea leaf extract, 12.5 parts of milk powder, 4.5 parts of oligoisomaltose, carboxymethyl chitosan 5
Part, 6 parts of xylo-oligosaccharide, 15 parts of tapioca starch, 3 parts of flavouring, 3.5 parts of sweet potato pectin and 3 parts of anticaking agent.
3. Zinc-rich saccharomyces cerevisiae solid beverage according to claim 1 or 2, it is characterised in that described Sweet tea leaf extract
Preparation method comprises the following steps：
1) take sweet tea to be crushed to 80 mesh, be put into the ethanol water that volume fraction is 60%, in 45 DEG C, 40KHz ultrasonic waves
Lower refluxing extraction 3 times, 15min is extracted every time, merge extract solution filtering and concentrating, obtain Sweet tea Leave extract；
2) add sodium carbonate to adjust pH to 8.8 in Sweet tea Leave extract obtained by step 1), filter to take supernatant；
3) supernatant obtained by step 2) is adjusted into pH to 7 with citric acid, adsorbing separation is carried out to it with D101 macroreticular resins, uses volume
The ethanol solution that fraction is 80% elutes, and takes eluent to be concentrated and dried and produces.
4. Zinc-rich saccharomyces cerevisiae solid beverage according to claim 1 or 2, it is characterised in that described milk powder by bovine colostrum,
Skimmed milk powder and whole-fat milk powder in mass ratio 1:4:5 compositions.
5. Zinc-rich saccharomyces cerevisiae solid beverage according to claim 1 or 2, it is characterised in that described flavouring is by glycyrrhizin
Element and stevioside in mass ratio 2:3 compositions.
6. Zinc-rich saccharomyces cerevisiae solid beverage according to claim 1 or 2, it is characterised in that the preparation of described sweet potato pectin
Method is：
(1) take sweet potato dregs 100g to add 3L water to stir, screening process 25min is carried out to it, collection can be 175 by aperture
μm screen cloth but can not be by the part of 50 μm of screen clothes, dry Rhizoma Dioscoreae esculentae dietary fiber；
(2) 1L water is added into Rhizoma Dioscoreae esculentae dietary fiber obtained by step (1), stir it is lower add 25g amylase, react after 20min from
Heart 25min, it is standby to collect precipitation；
(3) Na that 200ml concentration is 0.8mol/L is added in being precipitated obtained by step (2)2HPO4Solution, stir lower react
Supernatant is collected by centrifugation after 25min, distillation and concentration is drying to obtain.
7. Zinc-rich saccharomyces cerevisiae solid beverage according to claim 6, it is characterised in that the amylase described in step (2) is formed sediment by α
Powder enzyme and Pullulanase in mass ratio 2:3 compositions.
8. Zinc-rich saccharomyces cerevisiae solid beverage according to claim 1 or 2, it is characterised in that described anticaking agent is that crystallite is fine
Dimension element.
9. the preparation method of the Zinc-rich saccharomyces cerevisiae solid beverage according to claim any one of 1-8, it is characterised in that including with
Lower step：
S1, Zinc-rich saccharomyces cerevisiae is weighed in parts by weight, be Sweet tea leaf extract, milk powder, oligoisomaltose, carboxymethyl chitosan, low
Xylan, tapioca starch, flavouring, sweet potato pectin and anticaking agent are well mixed, and are added in trough type mixing machine, are turned on the power to be mixed
Water is added by the 5-15% of material total amount when agitating paddle rotates in machine, lower reaction 15-35min is stirred after closing the lid, obtains mixture；
S2, take 40 eye mesh screens to be installed in oscillating granulator, add mixture obtained by step S1, start granulator system
Grain；
S3, the step S2 particles made are sent to boiling drier, 45-55min is dried at 35-45 DEG C, is produced.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710774198.2A CN107581253A (en) | 2017-08-31 | 2017-08-31 | A kind of Zinc-rich saccharomyces cerevisiae solid beverage and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710774198.2A CN107581253A (en) | 2017-08-31 | 2017-08-31 | A kind of Zinc-rich saccharomyces cerevisiae solid beverage and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107581253A true CN107581253A (en) | 2018-01-16 |
Family
ID=61050671
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710774198.2A Pending CN107581253A (en) | 2017-08-31 | 2017-08-31 | A kind of Zinc-rich saccharomyces cerevisiae solid beverage and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107581253A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113785927A (en) * | 2021-09-07 | 2021-12-14 | 山西振东五和健康科技股份有限公司 | Yeast zinc solid beverage and preparation method thereof |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101485483A (en) * | 2009-03-02 | 2009-07-22 | 宋庆利 | Yeast beverage and method for preparing same |
| CN101627825A (en) * | 2008-07-15 | 2010-01-20 | 中国农业科学院农产品加工研究所 | Novel method for extracting pectin from sweet potato dregs |
| CN101756216A (en) * | 2008-12-16 | 2010-06-30 | 安琪酵母股份有限公司 | Yeast nutritious food and preparation method thereof |
| CN101766660A (en) * | 2008-12-30 | 2010-07-07 | 安琪酵母股份有限公司 | Preparation having function of improving memories and preparation method and application thereof |
| CN101829209A (en) * | 2010-04-28 | 2010-09-15 | 广西大学 | Preparation method of fruit-flavored sweet tea polyphenols |
| CN102232596A (en) * | 2010-12-24 | 2011-11-09 | 朱丽华 | Zinc supplementing drink |
| CN102838644A (en) * | 2012-08-23 | 2012-12-26 | 长沙绿蔓生物科技有限公司 | Production method for extracting sweet tea glucoside from sweet tea leaves |
| CN104687178A (en) * | 2015-03-13 | 2015-06-10 | 上海益力健营养品有限公司 | Solid beverage capable of being directly taken orally and preparation method thereof |
| JP2015130840A (en) * | 2014-01-15 | 2015-07-23 | キユーピー株式会社 | Powder food |
| CN104872755A (en) * | 2015-05-14 | 2015-09-02 | 谢光玉 | Zinc-supplementing beverage |
-
2017
- 2017-08-31 CN CN201710774198.2A patent/CN107581253A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101627825A (en) * | 2008-07-15 | 2010-01-20 | 中国农业科学院农产品加工研究所 | Novel method for extracting pectin from sweet potato dregs |
| CN101756216A (en) * | 2008-12-16 | 2010-06-30 | 安琪酵母股份有限公司 | Yeast nutritious food and preparation method thereof |
| CN101766660A (en) * | 2008-12-30 | 2010-07-07 | 安琪酵母股份有限公司 | Preparation having function of improving memories and preparation method and application thereof |
| CN101485483A (en) * | 2009-03-02 | 2009-07-22 | 宋庆利 | Yeast beverage and method for preparing same |
| CN101829209A (en) * | 2010-04-28 | 2010-09-15 | 广西大学 | Preparation method of fruit-flavored sweet tea polyphenols |
| CN102232596A (en) * | 2010-12-24 | 2011-11-09 | 朱丽华 | Zinc supplementing drink |
| CN102838644A (en) * | 2012-08-23 | 2012-12-26 | 长沙绿蔓生物科技有限公司 | Production method for extracting sweet tea glucoside from sweet tea leaves |
| JP2015130840A (en) * | 2014-01-15 | 2015-07-23 | キユーピー株式会社 | Powder food |
| CN104687178A (en) * | 2015-03-13 | 2015-06-10 | 上海益力健营养品有限公司 | Solid beverage capable of being directly taken orally and preparation method thereof |
| CN104872755A (en) * | 2015-05-14 | 2015-09-02 | 谢光玉 | Zinc-supplementing beverage |
Non-Patent Citations (3)
| Title |
|---|
| 王立波等: "甜茶活性成分最新研究进展", 《粮食与油脂》 * |
| 解彬等: "生物强化富锌食品的研究进展", 《大连民族大学学报》 * |
| 谭冬明等: "广西甜茶化学成分、提取工艺及生物活性研究进展", 《中成药》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113785927A (en) * | 2021-09-07 | 2021-12-14 | 山西振东五和健康科技股份有限公司 | Yeast zinc solid beverage and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104432084A (en) | Conditioning powder and preparation method and application thereof | |
| CN102524872B (en) | Ginseng peptide amino acid drink | |
| CN107319525A (en) | One kind fat-reducing fat reducing tailored version clinical nutrition formula and preparation method thereof | |
| CN106721789A (en) | A kind of oat compound linolenic acid beverage and preparation method thereof | |
| CN101536787A (en) | Sugar-free high-calcium albumen powder for improving senile osteoporosis | |
| CN104223119A (en) | Lepidium meyenii walp protein nutritional supplement for relieving physical fatigue and preparation method of lepidium meyenii walp protein nutritional supplement | |
| CN109567208A (en) | Enteral nutrition preparation and its preparation method and application containing marine bioactivity polysaccharide | |
| CN102349608A (en) | Healthcare product combination for clearing and nourishing throat and preparation method thereof | |
| CN102960807A (en) | Method for preparing coix seed polysaccharide beverage | |
| CN103142640A (en) | Health care product containing vitamin K2 and having functions of preventing and treating brittle-bone disease | |
| CN103392799B (en) | Castanea mollissima peptide lactic acid bacteria beverage and preparation method thereof | |
| CN109275778A (en) | A kind of women helps pregnant food formula of special diet and preparation method thereof | |
| CN107581253A (en) | A kind of Zinc-rich saccharomyces cerevisiae solid beverage and preparation method thereof | |
| CN104543652A (en) | Maca pollen and preparation method for maca pollen | |
| CN111771970A (en) | Composition beneficial to nourishing and protecting stomach and preparation method thereof | |
| CN111357822A (en) | Formula milk powder for promoting growth and development of height and preparation method thereof | |
| CN106306921A (en) | Method for preparing chitosan oligosaccharide functional beverage with Maca and Cordyceps sinensis and functional beverage made thereby | |
| CN105919074A (en) | Processing and making method of brewing powder and brewing powder processed and made by processing and making method | |
| CN108208813A (en) | A kind of weight-reducing with health role disappears fat piece and preparation method thereof | |
| CN113598377A (en) | Composition for improving anemia, functional food thereof, preparation method and application | |
| KR101440465B1 (en) | Ginseng diet shake | |
| CN102389109B (en) | Rice bran nutrient granule | |
| CN112314940A (en) | Composition for improving cramp symptom and preparation method thereof | |
| CN111903822A (en) | Calcium-supplementing growth-type sandwich gel candy and preparation method thereof | |
| CN107050147B (en) | Composition for bidirectionally improving gastrointestinal tract function and losing weight and beautifying and preparation thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180116 |