CN104543652A - Maca pollen and preparation method for maca pollen - Google Patents
Maca pollen and preparation method for maca pollen Download PDFInfo
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- CN104543652A CN104543652A CN201510004096.3A CN201510004096A CN104543652A CN 104543652 A CN104543652 A CN 104543652A CN 201510004096 A CN201510004096 A CN 201510004096A CN 104543652 A CN104543652 A CN 104543652A
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- 201000003152 motion sickness Diseases 0.000 description 1
- 238000010172 mouse model Methods 0.000 description 1
- OCSIXVSXYFNTQS-UHFFFAOYSA-N n-benzyloctanamide Chemical compound CCCCCCCC(=O)NCC1=CC=CC=C1 OCSIXVSXYFNTQS-UHFFFAOYSA-N 0.000 description 1
- 235000021049 nutrient content Nutrition 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 235000020733 paullinia cupana extract Nutrition 0.000 description 1
- 238000011056 performance test Methods 0.000 description 1
- 230000019612 pigmentation Effects 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 201000007094 prostatitis Diseases 0.000 description 1
- RADKZDMFGJYCBB-UHFFFAOYSA-N pyridoxal hydrochloride Natural products CC1=NC=C(CO)C(C=O)=C1O RADKZDMFGJYCBB-UHFFFAOYSA-N 0.000 description 1
- 235000020995 raw meat Nutrition 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 210000004927 skin cell Anatomy 0.000 description 1
- 239000011122 softwood Substances 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 235000003702 sterols Nutrition 0.000 description 1
- 150000003432 sterols Chemical class 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- 229960003080 taurine Drugs 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 230000035922 thirst Effects 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 150000003672 ureas Chemical class 0.000 description 1
- 238000010200 validation analysis Methods 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- 235000019158 vitamin B6 Nutrition 0.000 description 1
- 239000011726 vitamin B6 Substances 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
- 229940011671 vitamin b6 Drugs 0.000 description 1
- 230000008673 vomiting Effects 0.000 description 1
- 235000020097 white wine Nutrition 0.000 description 1
- 235000020985 whole grains Nutrition 0.000 description 1
- 239000000811 xylitol Substances 0.000 description 1
- 235000010447 xylitol Nutrition 0.000 description 1
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 1
- 229960002675 xylitol Drugs 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Preparation Of Fruits And Vegetables (AREA)
Abstract
The invention relates to maca pollen, relates to a preparation method for the maca pollen, and belongs to the technical field of health products. The maca pollen is prepared from the following raw materials in parts by weight: 20-30 parts of rape pollen, 40-60 parts of maca powder, 1-10 parts of lentinula edodes beef powder, 1-5 parts of yam powder, 1-10 parts of mango powder, 6-10 parts of pumpkin seed powder, 2-10 parts of isomaltitol and 1-6 parts of composite dietary fibers. The preparation method for the maca pollen comprises the following steps: accurately weighing the raw materials according to a formula, performing superfine grinding on the rape pollen, then mixing with the maca powder, adding the pumpkin seed powder, the entinula edodes beef powder, the yam powder, the mango powder and the composite dietary fibers in sequence, finally adding the isomaltitol, uniformly mixing and then performing superfine grinding until the fineness is 2-10 microns. According to the research, the rape pollen, the maca powder, the pumpkin seed powder and other raw materials are combined, so that the immunity is improved, the anti-fatigue is obvious, and the gastroenteric function is improved. The maca pollen can be mixed with water for drinking or can be used as a raw material.
Description
Technical field
The present invention relates to a kind of agate coffee pollen, and relate to the preparation method of this agate coffee pollen, belong to health product technology field.
Background technology
Agate coffee (Maca), have another name called agate card, originate in the mountain area, Andean, South America of height above sea level 3500 ~ 4500 meters, also known as Andean ginseng, mainly be distributed in the Puno ecotope in the middle part of Peru and Peru the southeastern city of Puno, for Cruciferae (Cruciferae) Lepidium (Lepidium) plant, formal name used at school Lepidium meyenii.Fresh agate coffee (MACA) moisture content 10.4%, protein 10.2%, fat 2.2%, carbohydrate 59%, fiber 8.5%, ash content 4.9%, containing abundant essential amino acid, as arginine 9.94%, asparatate 9.17%, glutamic acid 15.6% and lysine 54.5%, in aliphatic acid, the content of unrighted acid accounts for 52.7%, and containing glucosinolate (glucosinolates) sterol and potassium, calcium, iron etc.Agate coffee alkene (macaenes) and macamide (mecamides) are contained in agate coffee (MACA) lipid position, also comprise N-benzyl caprylamide, N-benzyl-16-hydroxyl-9-oxidation-10E, 12E, 14E-pungent certain herbaceous plants with big flowers triolefin acid amides, N-benzyl-16 hydroxyl-9,16-dioxy-10E, the homologue of 12E, 14E-pungent certain herbaceous plants with big flowers triolefin acid amides and other 17 agate coffee alkene and macamide.Agate coffee alkene and macamide are considered to one of active principle of tool promotion property function in agate coffee (MACA) extract.From phase early 1960s to the eighties, about the research progressively systematization of agate coffee botany and medical value, some traditional role of agate coffee obtain the scientific validation of Germany and North American Plant researcher.Not only can fill the stomach, also can increase the ability of muscle power, endurance and antifatigue.Therefore, more to the research of agate coffee, be commonly used to production health drink or health liquor.
The Chinese invention " Maca beverage " of application number 201210515910.4; disclose one and comprise following raw material Maca beverage: 0.02%-0.1% pueraria root powder; 0.3%-1.0% ginseng; 0.02%-0.1% Chinese yam; 0.2%-0.6% chrysanthemum; 0.2%-0.6% honeysuckle; 0.04%-0.06% Guarana extract, 0.01%-0.1% Soybean Peptide, 0.01%-0.04% coffee-extract; 0.4%-0.6% taurine; 0.06%-0.12% inositol, 2.0%-3.0% vitamin B1,0.4%-1.2% vitamin B6; and appropriate local flavor conditioning agent, all the other are water.Maca beverage of the present invention have refresh oneself, antifatigue, quickly supplement energy, regain one's strength, effect of heat-clearing.
Application number 201410194504.1 " Maca wine " relates to a kind of Maca wine, is made up of the raw material of following weight portion: pueraria root powder 200 ~ 300 parts, Shorthorned Epimedium P.E 50 ~ 100 parts, arginine 100 ~ 200 parts, matrimony vine 60 ~ 150 parts; Base wine is the white wine 900 ~ 1500 parts of 50% alcoholic strength.Utilizing the present invention to prepare in Maca wine process owing to pueraria root powder to be broken into granularity is 20 object powder, is exposed in base wine by the functional component of agate coffee to greatest extent, makes its abundant stripping, effectively utilize agate coffee, comprehensive nutrition; Prepare wine distillation reuse residual in the dregs of a decoction of Maca wine, cost-saving, reduce environmental pollution.
Rape is the agricultural economy crop of China's establishing in large scale, powder source is wide, quality is pure, rape pollen contains abundant functional materials and comprises protein, vitamin, trace element, flavone compound, these materials act synergistically on body, regulate the several functions of body, balance endotrophic, strengthen metabolism, reduce capillary permeability, there is kidney-nourishing, consolidate, effect of strong waist, significant curative effect is had to prostatitis and hyperplasia of prostate, and there is antiatherosclerosis, treatment varicose ulcer, reduce cholesterol and radiation-resistant effect, the growth of incretory can also be promoted, improve and regulate incretory secreting function, therefore to the disease caused by endocrine dysfunction, there is good adjuvant treatment effect.And the cosmetic result of Bee Pollen is more obvious, the balance of human body internal system can be regulated comprehensively, by inner and table, fundamentally improve the vigor of Skin Cell, strengthen the metabolism of skin, prevent facial pigmentation, pachylosis, aging, make skin keep moistening, pure white, glossy, high resilience.
The Chinese invention " a kind of agate coffee pollen buccal tablet of caf " of application number 201310455218.1 discloses a kind of agate coffee pollen buccal tablet of caf, with pueraria root powder and pollen for functional component, be aided with other acceptable auxiliary material to be prepared from, described auxiliary material is ground coffee, sweetener and lubricant, its percentage by weight is 10%-30% pueraria root powder, 10%-30% pollen, 5%-15% ground coffee, 30%-70% sweetener and 0.5%-1% lubricant.By human body antifatigue effect test and mouse anti-reflecting fatigue efficacy test, there is adjustment internal system, balance hormone; Strengthen human activity, release the pressure and fatigue effect, can be used for the purposes of preventing or treatment is tired, male's sexual declines.
The Chinese invention " a kind of formula of the agate coffee trehalose chankings for women and preparation technology " of application number 201410009482.7 discloses a kind of formula and preparation technology of the agate coffee trehalose chankings for women, the content of agate coffee is adopted to account for the 18%-42% of candy sheet weight, the content of pollen pini accounts for the 8%-20% of candy sheet weight, the content of microcrystalline cellulose accounts for the 22%-39% of candy sheet weight, the content of xylitol accounts for the 18%-35% of candy sheet weight, the content of citric acid accounts for the 0.5%-4% of candy sheet weight, the content of dolomol accounts for the 0.5%-3% of candy sheet weight, 10% dextrin slurry formula and by drying, beat powder → Homogeneous phase mixing, make softwood → drying, sieve whole grain → compressing tablet and make the chankings → inspection of agate coffee trehalose, the flow process of packaging → packaging warehouse-in.The candy sheet utilizing the present invention to make not only fill a prescription and technological process simple, and good mouthfeel, absorption fast, carry, convenient transportation.
Though each tool advantage of foregoing invention, but or agate coffee content is lower, or range of application is smaller, especially namely agate coffee uses after being powder process simply, all do not relate to the meticulous preparation of agate coffee, so that in product, effective component in Maka content is lower, and trophic component is unreasonable, the deep development that have impact on agate coffee utilizes.
Summary of the invention
For solving the problem, the invention provides a kind of trophic component balanced and reasonable, active constituent content is high, absorption easy to digest, and the agate coffee pollen that taste flavor is good, and the preparation method of this agate coffee pollen is provided.
Described agate coffee pollen comprises the raw material composition of following parts by weight: rape pollen 20-30 part, pueraria root powder 40-60 part, mushroom powdered beef 1-10 part, yam flour 1-5 part, mango powder 1-10 part, pumpkin seed powder 6-10 part, isomalt 2-10 part, composite dietary 1-6 part;
Preferably, described pueraria root powder preparation method comprises the steps:
After agate coffee impurity elimination cleaning, 5 times of soft water are added by agate coffee weight, after corase grind grinding, add the soft water of agate coffee weight 8 times, and add pumpkin seed powder, addition counts 1-2% with agate coffee weight, grind through colloid mill after mixing, slurries add mixed enzyme and carry out enzymolysis, pH5.2-5.5, temperature 45-50 DEG C, enzymolysis time 3-4hr, described mixed enzyme consumption is the 0.05-0.2% of slurry weight, and described mixed enzyme parts by weight consist of: pectase 4 parts, 1,4 beta-glucanase 1 part, 2 parts, protease and cellulase 2 parts.Enzymolysis liquid goes out enzyme, filtration, pulverizes and get final product after freeze concentration, freeze drying.
Described rape pollen is market products;
Described isomalt is market products;
Described composite dietary comprises the composition of following parts by weight: xylo-oligosaccharide 1 part, Semen Tritici aestivi fiber 1.5 parts, fibre and soya 0.5 part;
Preferably, described mushroom powdered beef is adopted and is prepared with the following method:
Mushroom process: select dried thin mushroom, cleans up, and 2-3 times of 60-70 DEG C of emerge in worm water 20-40min, is cooled to normal temperature rapidly, and after Separation of Solid and Liquid, bubble mushroom water retains for subsequent use, and all adds in mixed material in subsequent handling;
-1-1 DEG C of degree is refrigerated to after beef cleaning, rub together with mushroom, add the water (comprising all steeping mushroom water) of mixed material 1-2 times weight, the gap of adjustment colloid mill stator and rotor is 60 ~ 120 microns, colloid mill flow-control is 0.1 ~ 0.3 ton/hour and makes mixing gravy, adjustment pH is 8-10, add the trypsase of gravy weight 0.1-0.25% and the alkali protease of 0.05-0.1%, 37-45 DEG C of mixed enzymolysis 1-2h, adjust pH to 5-6.5, be warming up to 50-58 DEG C, add the compound fertilizer production enzymolysis 3-4h of gravy weight 0.2-0.3%, go out enzyme, homogeneous, concentrated, crushed after being dried and get final product,
The weight ratio of described beef and mushroom is: beef: mushroom=10-15:1-3.
Preferably, described yam flour is adopted and is prepared with the following method:
Chinese yam is removed the peel, weigh, stripping and slicing, defibrination, add 18-20 times of soft water according to peeling Chinese yam weight and carry out the extraction of microwave cooperating ultrasonic wave, microwave power is 500-800W, frequency 2450MHz; Ultrasonic power 800-1000W, frequency 40KHz, the time is 15-45min; It is 80% that extract adds absolute ethyl alcohol to solution alcohol concentration, filters after alcohol precipitation 14hr; Filter residue is dissolved in 3-4 times of pure water, Vacuum Concentration, and vacuum drying lower than 5%, crosses 80-100 mesh sieve after pulverizing to moisture.
Preferably, described mango powder is adopted and is prepared with the following method:
Mango cleaning peeling stoning, after adding pulp weight 6-12 colour protecting liquid making beating doubly, the pH value controlling solution is 4-5, add with the complex enzyme of pulp weighing scale 0.1-0.5%, described complex enzyme is made up of acid pectase, neutral proteinase and cellulase, the weight ratio of three kinds of enzymes is 2.5: 1.5: 1, and hydrolysis temperature is 50-53 DEG C, and enzymolysis time is 1.5-3hr; Afterwards pH value is adjusted to 5.1-5.6, temperature is raised to 55-60 DEG C, adds with the dextranase of the pectase of pulp weighing scale 0.2-0.5%, 0.2-0.6% simultaneously, and action time is 2.5-3hr; Afterwards by enzymolysis liquid under 0.6A/25W-1.5A/275W condition, ultrasonic extraction 20-30min, extract is 5-10 DEG C of filtration; Filtrate freeze concentration, freeze drying, pulverizes and obtains mango powder;
The aqueous ascorbic acid of described colour protecting liquid to be pH be 0.15% of 6.5-6.8;
Preferably, described filtration, medium is the mixture of diatomite and hickory chick powder, and in volume of material to be filtered, food grade kieselguhr use amount is: 10-50g/L, and hickory chick powder use amount is 0.1-0.2g/L.
Preferably, described pumpkin seed powder is that after pumpkin seeds impurity elimination, belt leather is broken through air blast ultramicro powder, makes fineness reach 2-10 micron and get final product;
Described agate coffee pollen is prepared by the following method:
By each raw material of formula precise, mix after rape pollen ultramicro grinding with pueraria root powder, and add pumpkin seed powder, mushroom powdered beef, yam flour, mango powder, composite dietary successively, finally add the rear ultramicro grinding of isomalt mixing, make fineness reach 2-10 micron.
Described agate coffee pollen favorable solubility, can reconstitute and directly to drink afterwards, pure taste, without smell, every day, the highest edible limitation, was no more than 1g/kgBW; Infant, children, women breast-feeding their children, pregnant woman are inapplicable.Described agate coffee pollen also can be used as raw material, for food processing.
Beneficial effect:
Pollen contains abundant functional materials and comprises protein, vitamin, trace element, flavone compound, these materials act synergistically on body, regulate the several functions of body, balance endotrophic, strengthen metabolism, prevent the obstacle of capillary permeability.Pollen can also promote the growth of incretory, is improved and regulates incretory secreting function, therefore serves therapeutic action to the disease caused by endocrine dysfunction.Rape pollen kidney-nourishing, consolidate, strong waist, and higher containing flavonols, there is antiatherosclerosis, treatment varicose ulcer, reduce cholesterol and radiation-resistant effect.Agate coffee has the effect increasing muscle power, endurance and antifatigue, and is improved sperm quality, regains one's strength of body, improving water flood, anti-climacteric, and active fertility, strengthens the effects such as memory.The present invention studies discovery, by rape pollen, pueraria root powder, pumpkin seed powder, and other material combination, the effect of raising immunity, antifatigue is especially obvious, and with improving the effect of gastrointestinal function.
Containing carrotene, white of an egg matter in pumpkin seeds, zinc and the necessary multiple nutritional components of the human body such as vitamin A, B1, B2, pumpkin seed powder is coordinated according to a certain percentage with pueraria root powder, and add a certain amount of pumpkin seeds in the enzyme-squash techniqued process of pueraria root powder, the activity of various enzyme can be promoted, unexpected effect is brought to raising agate coffee enzymolysis efficiency.Especially by pumpkin seeds belt leather ultramicro grinding to 2-10 micron, granularity evenly, and crushing process does not have associated heat, and it is low to pulverize temperature rise, and nutrition is preserved completely, and absorption more easy to digest.
Mango taste is sweet, sour, cool in nature, useful stomach preventing or arresting vomiting, promotes the production of body fluid and quenches one's thirst, pre-carsickness-proof, anti-inflammation, bright eyes, the effects such as cancer-resisting.Adopt the mode that enzymolysis combines with ultrasonic extraction, special employing diatomite and the mixing of hickory chick powder, as filter medium, are filtered under low temperature, while removing impurity, the nutrition of Absorbable rod hickory chick powder and flavor substance again, makes final obtained mango powder have unique fragrance and local flavor.
Carry out enzymolysis according to special process after beef mixes according to a certain percentage with mushroom, while guarantee higher nutrient content, the local flavor of mushroom and beef merged mutually, complementary, the fresh fragrant uniqueness except raw meat of going to have a strong smell.
By water-soluble dietary fiber and Water insoluble dietary fiber compound, can Constipation weaken the toxin that bacterium in alimentary canal discharges, promote beneficial bacterium field planting.Isomalt has the excellent characteristic such as high stability, agent of low hygroscopicity, the more important thing is that isomalt is a kind of excellent Bifidobacterium MF, act synergistically with rational proportion dietary fiber and can play coordinating intestines and stomach, improve gastrointestinal function, the absorption of agate coffee pollens nutrition and functional component can be promoted, the absorption of the trace elements such as calcium iron can not be affected again simultaneously.
Agate coffee pollens nutrition composition of the present invention is reasonable, and active constituent content is higher, and each component combines according to special ratios, mutual benifit, and synergy, shows good antifatigue effect.Mango powder, mushroom powdered beef provide balanced nutritious while, local flavor and the mouthfeel of agate coffee pollen can be improved.Again through ultramicro grinding after each raw material mixing, each component is mixed better, and the digestibility of product of the present invention can be improved, the final health-care effect improving product of the present invention.
Detailed description of the invention
Embodiment 1
Described agate coffee pollen is made up of the raw material of following parts by weight: rape pollen 25 parts, pueraria root powder 60 parts, mushroom powdered beef 5 parts, yam flour 3 parts, mango powder 6 parts, pumpkin seed powder 8 parts, isomalt 6 parts, composite dietary 4 parts;
Described pueraria root powder preparation method comprises the steps:
After agate coffee impurity elimination cleaning, add 5 times of soft water by agate coffee weight, after corase grind grinding, add the soft water of agate coffee weight 8 times, and adding pumpkin seed powder, addition counts 2% with agate coffee weight, grinds after mixing through colloid mill, slurries add mixed enzyme and carry out enzymolysis, pH5.3, temperature 46 DEG C, enzymolysis time 3.5hr, described mixed enzyme consumption is 0.1% of slurry weight, and described mixed enzyme parts by weight consist of: pectase 4 parts, 1,4 beta-glucanase 1 part, 2 parts, protease and cellulase 2 parts.Enzymolysis liquid goes out enzyme, filtration, pulverizes and get final product after freeze concentration, freeze drying.
Described rape pollen is market products;
Described isomalt is market products;
Described composite dietary comprises the composition of following parts by weight: xylo-oligosaccharide 1 part, Semen Tritici aestivi fiber 1.5 parts, fibre and soya 0.5 part;
Described mushroom powdered beef is adopted and is prepared with the following method:
Mushroom process: select dried thin mushroom, cleans up, and 3 times of 65 DEG C of emerge in worm water 30min, are cooled to normal temperature rapidly, and after Separation of Solid and Liquid, bubble mushroom water retains for subsequent use;
-1 DEG C of degree is refrigerated to after beef cleaning, rub together with mushroom, add the water (comprising all steeping mushroom water) of mixed material 2 times of weight, the gap of adjustment colloid mill stator and rotor is 100 microns, colloid mill flow-control is 0.2 ton/hour and makes mixing gravy, adjustment pH is 9, add the trypsase of gravy weight 0.1% and the alkali protease of 0.1%, 41 DEG C of mixed enzymolysis 2h, adjust pH to 6.0, be warming up to 54 DEG C, add the compound fertilizer production enzymolysis 3h of gravy weight 0.3%, go out enzyme, homogeneous, concentrated, crushed after being dried and get final product;
The weight ratio of described beef and mushroom is: beef: mushroom=10:3.
Described yam flour is adopted and is prepared with the following method:
Chinese yam is removed the peel, weigh, stripping and slicing, defibrination, add 18 times of soft water according to peeling Chinese yam weight and carry out the extraction of microwave cooperating ultrasonic wave, microwave power is 650W, frequency 2450MHz; Ultrasonic power 900W, frequency 40KHz, the time is 30min; It is 80% that extract adds absolute ethyl alcohol to solution alcohol concentration, filters after alcohol precipitation 14hr; Filter residue is dissolved in 4 times of pure water, Vacuum Concentration, and vacuum drying lower than 5%, crosses 80 mesh sieves after pulverizing to moisture.
Described mango powder is adopted and is prepared with the following method:
Mango cleaning peeling stoning, after adding the colour protecting liquid making beating of pulp weight 8 times, the pH value controlling solution is 4.5, add with the complex enzyme of pulp weighing scale 0.3%, described complex enzyme is made up of acid pectase, neutral proteinase and cellulase, the weight ratio of three kinds of enzymes is 2.5: 1.5: 1, and hydrolysis temperature is 50 DEG C, and enzymolysis time is 3hr; Afterwards pH value is adjusted to 5.1, temperature is raised to 55 DEG C, add simultaneously with the pectase of pulp weighing scale 0.5%, 0.2% dextranase, action time is 3hr; Afterwards by enzymolysis liquid under 1.5A/275W condition, ultrasonic extraction 20min, extract is 5 DEG C of filtrations; Filtrate freeze concentration, freeze drying, pulverizes and obtains mango powder;
The aqueous ascorbic acid of described colour protecting liquid to be pH be 0.15% of 6.5-6.8;
Described filtration, medium is the mixture of diatomite and hickory chick powder, and in volume of material to be filtered, food grade kieselguhr use amount is: 30g/L, and hickory chick powder use amount is 0.1g/L.
Described pumpkin seed powder is that after pumpkin seeds impurity elimination, belt leather is broken through air blast ultramicro powder, makes fineness reach 2-10 micron and get final product;
Described agate coffee pollen is prepared by the following method:
By each raw material of formula precise, mix after rape pollen ultramicro grinding with pueraria root powder, and add pumpkin seed powder, mushroom powdered beef, yam flour, mango powder, composite dietary successively, finally add the rear ultramicro grinding of isomalt mixing, make fineness reach 2-10 micron.
Embodiment 2
Described agate coffee pollen is made up of the raw material of following parts by weight: rape pollen 20 parts, pueraria root powder 40 parts, mushroom powdered beef 10 parts, yam flour 1 part, mango powder 10 parts, pumpkin seed powder 10 parts, isomalt 10 parts, composite dietary 6 parts, wolfberry fruit powder 1 part;
Described pueraria root powder is market products;
Described rape pollen is market products;
Described isomalt is market products;
Described wolfberry fruit powder is market products;
Described composite dietary comprises the composition of following parts by weight: xylo-oligosaccharide 1 part, Semen Tritici aestivi fiber 1.5 parts, fibre and soya 0.5 part;
Described mushroom powdered beef is adopted and is prepared with the following method:
Mushroom process: select dried thin mushroom, cleans up, and 2 times of 60 DEG C of emerge in worm water 40min, are cooled to normal temperature rapidly, and after Separation of Solid and Liquid, bubble mushroom water retains for subsequent use;
1 DEG C of degree is refrigerated to after beef cleaning, rub together with mushroom, add the water (comprising all steeping mushroom water) of mixed material 1 times of weight, the gap of adjustment colloid mill stator and rotor is 60 microns, colloid mill flow-control is 0.1 ton/hour and makes mixing gravy, adjustment pH is 8, add the trypsase of gravy weight 0.25% and the alkali protease of 0.1%, 37 DEG C of mixed enzymolysis 1h, adjust pH to 5, be warming up to 50 DEG C, add the compound fertilizer production enzymolysis 4h of gravy weight 0.2%, go out enzyme, homogeneous, concentrated, crushed after being dried and get final product;
The weight ratio of described beef and mushroom is: beef: mushroom=15:1.
Described yam flour is adopted and is prepared with the following method:
Chinese yam is removed the peel, weigh, stripping and slicing, defibrination, add 18 times of soft water according to peeling Chinese yam weight and carry out the extraction of microwave cooperating ultrasonic wave, microwave power is 500W, frequency 2450MHz; Ultrasonic power 1000W, frequency 40KHz, the time is 45min; It is 80% that extract adds absolute ethyl alcohol to solution alcohol concentration, filters after alcohol precipitation 14hr; Filter residue is dissolved in 3 times of pure water, Vacuum Concentration, and vacuum drying lower than 5%, crosses 80 mesh sieves after pulverizing to moisture.
Described mango powder is adopted and is prepared with the following method:
Mango cleaning peeling stoning, after adding the colour protecting liquid making beating of pulp weight 12 times, the pH value controlling solution is 5, add with the complex enzyme of pulp weighing scale 0.5%, described complex enzyme is made up of acid pectase, neutral proteinase and cellulase, the weight ratio of three kinds of enzymes is 2.5: 1.5: 1, and hydrolysis temperature is 53 DEG C, and enzymolysis time is 1.5hr; Afterwards pH value is adjusted to 5.3, temperature is raised to 55 DEG C, add simultaneously with the pectase of pulp weighing scale 0.2%, 0.6% dextranase, action time is 2.5hr; Afterwards by enzymolysis liquid under 0.6A/25W condition, ultrasonic extraction 30min, extract is 10 DEG C of filtrations; Filtrate freeze concentration, freeze drying, pulverizes and obtains mango powder;
The aqueous ascorbic acid of described colour protecting liquid to be pH be 0.15% of 6.5-6.8;
Described pumpkin seed powder is that after pumpkin seeds impurity elimination, belt leather is broken through air blast ultramicro powder, makes fineness reach 2-10 micron and get final product;
Described agate coffee pollen is prepared by the following method:
By each raw material of formula precise, mix after rape pollen ultramicro grinding with pueraria root powder, and add pumpkin seed powder, mushroom powdered beef, yam flour, mango powder, composite dietary successively, finally add the rear ultramicro grinding of isomalt mixing, make fineness reach 2-10 micron.
Embodiment 3
Described agate coffee pollen is made up of the raw material of following parts by weight: rape pollen 30 parts, pueraria root powder 50 parts, mushroom powdered beef 1 part, yam flour 5 parts, mango powder 10 parts, pumpkin seed powder 6 parts, isomalt 10 parts, composite dietary 3 parts;
Described pueraria root powder preparation method comprises the steps:
After agate coffee impurity elimination cleaning, add 5 times of soft water by agate coffee weight, after corase grind grinding, add the soft water of agate coffee weight 8 times, and adding pumpkin seed powder, addition counts 2% with agate coffee weight, grinds after mixing through colloid mill, slurries add mixed enzyme and carry out enzymolysis, pH5.5, temperature 50 C, enzymolysis time 3.5hr, described mixed enzyme consumption is 0.12% of slurry weight, and described mixed enzyme parts by weight consist of: pectase 4 parts, 1,4 beta-glucanase 1 part, 2 parts, protease and cellulase 2 parts.Enzymolysis liquid goes out enzyme, filtration, pulverizes and get final product after freeze concentration, freeze drying.
Described rape pollen is market products;
Described isomalt is market products;
Described composite dietary comprises the composition of following parts by weight: xylo-oligosaccharide 1 part, Semen Tritici aestivi fiber 1.5 parts, fibre and soya 0.5 part;
Described mushroom powdered beef is adopted and is prepared with the following method:
Mushroom process: select dried thin mushroom, cleans up, and 2 times of 70 DEG C of emerge in worm water 20min, are cooled to normal temperature rapidly, and after Separation of Solid and Liquid, bubble mushroom water retains for subsequent use;
-1 DEG C of degree is refrigerated to after beef cleaning, rub together with mushroom, add the water (comprising bubble mushroom water) of mixed material 2 times of weight, the gap of adjustment colloid mill stator and rotor is 120 microns, colloid mill flow-control is 0.3 ton/hour and makes mixing gravy, adjustment pH is 10, add the trypsase of gravy weight 0.2% and the alkali protease of 0.05%, 45 DEG C of mixed enzymolysis 1h, adjust pH to 6.5, be warming up to 58 DEG C, add the compound fertilizer production enzymolysis 3h of gravy weight 0.2%, go out enzyme, homogeneous, concentrated, crushed after being dried and get final product;
The weight ratio of described beef and mushroom is: beef: mushroom=10:3.
Described yam flour is adopted and is prepared with the following method:
Chinese yam is removed the peel, weigh, stripping and slicing, defibrination, add 20 times of soft water according to peeling Chinese yam weight and carry out the extraction of microwave cooperating ultrasonic wave, microwave power is 800W, frequency 2450MHz; Ultrasonic power 800W, frequency 40KHz, the time is 15min; It is 80% that extract adds absolute ethyl alcohol to solution alcohol concentration, filters after alcohol precipitation 14hr; Filter residue is dissolved in 4 times of pure water, Vacuum Concentration, and vacuum drying lower than 5%, crosses 100 mesh sieves after pulverizing to moisture.
Described mango powder is adopted and is prepared with the following method:
Mango cleaning peeling stoning, after adding the colour protecting liquid making beating of pulp weight 6 times, the pH value controlling solution is 5, add with the complex enzyme of pulp weighing scale 0.1%, described complex enzyme is made up of acid pectase, neutral proteinase and cellulase, the weight ratio of three kinds of enzymes is 2.5: 1.5: 1, and hydrolysis temperature is 53 DEG C, and enzymolysis time is 3hr; Afterwards pH value is adjusted to 5.6, temperature is raised to 60 DEG C, add simultaneously with the pectase of pulp weighing scale 0.5%, 0.6% dextranase, action time is 3hr; Afterwards by enzymolysis liquid under 1.5A/275W condition, ultrasonic extraction 20min, extract is 5 DEG C of filtrations; Filtrate freeze concentration, freeze drying, pulverizes and obtains mango powder;
The aqueous ascorbic acid of described colour protecting liquid to be pH be 0.15% of 6.5-6.8;
Described filtration, medium is the mixture of diatomite and hickory chick powder, and in volume of material to be filtered, food grade kieselguhr use amount is: 50g/L, and hickory chick powder use amount is 0.2g/L.
Described pumpkin seed powder is that after pumpkin seeds impurity elimination, belt leather is broken through air blast ultramicro powder, makes fineness reach 2-10 micron and get final product;
Described agate coffee pollen is prepared by the following method:
By each raw material of formula precise, mix after rape pollen ultramicro grinding with pueraria root powder, and add pumpkin seed powder, mushroom powdered beef, yam flour, mango powder, composite dietary successively, finally add the rear ultramicro grinding of isomalt mixing, make fineness reach 2-10 micron.
Experimental example 1 mouse intestinal performance test
With agate coffee pollen prepared by the embodiment of the present invention 1,2,3, do following test;
Choose common Kunming small white mouse 150, male and female half and half, body weight 18-22g, conventional word is supported.Therefrom random choose 120,9:00 gavage Lincomycin Hydrochloride 0.2mL every morning (20mg)/only, continuous one week, prepares the mouse model of intestinal bacilli illness, other as a control group, every day same time gavage equivalent sterile saline.Model group mouse diet declines, and do not occur dead and phenomenon of significantly suffering from diarrhoea, arrange soft excrement, profile normal aqueous divides more, and bedding and padding are moist.By 120 intestinal bacilli illness mouse, be divided into 4 groups at random, often organize 30, group 1, group 2, group 3 are as treatment group, and dosage presses human body the highest edible limitation 1g/kgBW every day, and embodiment 1,2,3 agate coffee pollen is carried out gavage with distilled water preparation, gavage amount is 0.4ml/20g, organize 4 as natural recovering group, every day same time gavage equivalent sterile saline, continuous two weeks.21 days whole experimental periods, observe growth and the defecation situation of small white mouse every day, weigh in the 8th, 21 day mouse to each group, calculate each group of weight average growth rate, result is as table 1; Within every 5 days, survey each group of stool in mice Escherichia coli quantity, calculate average, result is as table 2.Get stool in mice and be about 0.1g, in aseptic operating platform, add 3 beades (adding 0.5mL dilution with 0.1g excrement sample), dilute and inoculate maconkey agar culture medium, calculate every gram of coliform count wet just.
Each group # is corresponding as follows: group 1-embodiment 1, group 2-embodiment 2, group 3-embodiment 3, group 4-natural recovering group
Table 1 mouse Gain weight
| Grouping | Average starting weight (g/ only) | Average end heavy (g/ only) | Average growth rate (%) |
| Group 1 | 20.41±1.42 | 30.89±1.33 | 51.33 |
| Group 2 | 20.52±1.35 | 29.12±1.56 | 41.91 |
| Group 3 | 20.61±1.38 | 30.13±1.45 | 46.19 |
| Group 4 | 20.69±1.33 | 27.34±1.59 | 32.14 |
The situation of coliform count in table 2 stool in mice
| Grouping | Initially (× 10 5cfu/g) | Terminate (× 10 5cfu/g) | Reduced rate (%) |
| Group 1 | 5.61±0.25 | 1.12±0.08 | 80.04 |
| Group 2 | 5.63±0.12 | 1.92±0.25 | 65.90 |
| Group 3 | 5.89±0.34 | 1.96±0.16 | 66.72 |
| Group 4 | 5.62±0.53 | 4.34±0.19 | 22.78 |
Treatment group Mouse Weight average growth rate is respectively 51.33%, 41.91%, 46.19%, is all significantly higher than 32.14% of natural recovering group; Agate coffee pollen rear intestinal Escherichia coli quantity of feeding significantly declines, reduce by 80.04%, 65.90%, 66.72% respectively, reduced rate is also significantly higher than 22.78% of natural recovering group, show that agate coffee pollen of the present invention effectively can suppress the growth and breeding of the pathogens such as Escherichia coli, regulate mouse intestinal flora, effectively improve enteron aisle performance.
Experimental example 2
Exercise tolerance is tested
1. experiment purpose
By exercise tolerance test (mouse forced swimming), observe various embodiments of the present invention product and single component and various combination to the impact of antifatigue effect, for determining that formula consumption provides experimental basis.
2. experiment material and reagent
2.1 experimental group numbering and components
G1: rape pollen 25g;
G2: pueraria root powder 60g;
G3: mushroom powdered beef 5g;
G4: yam flour 3g;
G5: mango powder 6g;
G6: pumpkin seed powder 8g;
G7: composite dietary 4g;
G8: isomalt 6g;
G9: pueraria root powder 60g, mushroom powdered beef 5g;
G10: pueraria root powder 60g, rape pollen 25g;
G11: pueraria root powder 60g, composite dietary 4g;
G12: pueraria root powder 60g, pumpkin seed powder 8g;
G13: pueraria root powder 60g, yam flour 3g;
G14: pueraria root powder 60g, mango powder 6g;
G15: pueraria root powder 60g, isomalt 6g;
G16: pueraria root powder 60g, rape pollen 25g, mushroom powdered beef 5g;
G17: pueraria root powder 60g, mushroom powdered beef 5g, composite dietary 4g;
G18: pueraria root powder 60g, rape pollen 25g, pumpkin seed powder 8g;
G19: pueraria root powder 60g, rape pollen 25g, mushroom powdered beef 5g, composite dietary 4g;
G20: pueraria root powder 60g, rape pollen 25g, pumpkin seed powder 8g, yam flour 3g;
G21: pueraria root powder 60g, rape pollen 25g, mushroom powdered beef 5g, pumpkin seed powder 8g, yam flour 3g;
G22: pueraria root powder 60g, rape pollen 25g, mushroom powdered beef 5g, pumpkin seed powder 8g, composite dietary 4g, yam flour 3g;
G23: pueraria root powder 60g, rape pollen 25g, mushroom powdered beef 5g, pumpkin seed powder 8g, composite dietary 4g, yam flour 3g, mango powder 6g;
G24: pueraria root powder 60g, rape pollen 25g, mushroom powdered beef 5g, pumpkin seed powder 8g, composite dietary 4g, yam flour 3g, mango powder 6g, isomalt 6g;
G25: pueraria root powder 40g, rape pollen 20g, mushroom powdered beef 10g, pumpkin seed powder 10g, composite dietary 6g, yam flour 1g, mango powder 10g, isomalt 10g;
G26: pueraria root powder 50g, rape pollen 30g, mushroom powdered beef 1g, pumpkin seed powder 6g, composite dietary 3g, yam flour 5g, mango powder 10g, isomalt 10g;
2.2 reagent:
Liver/muscle glycogen testing cassete, purchased from abundant Bioisystech Co., Ltd of upper Hisense;
The concentrated sulfuric acid (AR), Beijing chemical reagent factory;
Physiological saline, Shangdong Changfu Jiejing Pharmaceutical Industry Co., Ltd..
3. animal used as test
The Kunming healthy male mice selecting Test Animal Centre, Academy of Military Medical Sciences, P.L.A to breed, cleaning grade, body weight 18-22g, the free diet of experimental session mouse.
First in advance swimming with a load attached to the body screening is carried out to mouse before experiment, the galvanized wire of root of the tail portion load 5% body weight, depth of water 40cm, water temperature 20 DEG C ± 0.5 DEG C, record mouse sinks to time that underwater 5s do not emerge as mice burden swimming time (min) from overboard beginning to nostril, filter out the close mouse of swimming time as experiment mice.
4. key instrument
Swimming trunk (60cm × 60cm × 40cm), galvanized wire, Allegra X-15R tabletop refrigerated centrifuge, Beckman coulter company; Water-bath: DZKW-C, Huanghua City spaceflight apparatus factory; Electronic balance: FA2004, the lucky Electronics Equipment Co., Ltd of Changzhou; Stopwatch, thermometer;
5. experiment grouping
5.1 dosage groupings and given the test agent give the time
At random mouse is divided into 27 groups, often organizes 10, G1-G26 group gavage correspondence group sample, dosage presses human body the highest edible limitation 1g/kgBW every day, by gavage after distilled water diluting, G27 group is blank group, gives isopyknic distilled water, gavage 1 time/d, gavage amount 0.4ml/20g, continuous 30 days.
5.2 sample preparation G1-G26: take 7.5g sample, be assigned to 150ml with distilled water;
Blank group: distilled water 150ml.
6. experimental technique
After 6.1 swimming with a load attached to the body experiment last administration 30min, put mouse in swimming trunk, depth of water 40cm, water temperature 20 DEG C ± 0.5 DEG C, the galvanized wire of mouse root of the tail portion load 5% body weight, record mouse sinks to time that underwater 5s do not emerge as mice burden swimming time (min) from overboard beginning to nostril.
After 6.2 mice serum urea measure last administration 30min, be not swimming with a load attached to the body 90min in the water of 30 DEG C in temperature, pluck eyeball blood sampling 0.5mL (not adding anti-coagulants) after rest 60min, put 4 DEG C of refrigerator 3h, after blood clotting, the centrifugal 15min of 2000r/min, gets Virus monitory.
After the mensuration last administration 30min of 6.3 hepatic glycogen, be not swimming with a load attached to the body 90min in the water of 25 ± 1 DEG C in temperature, cervical dislocation puts to death mouse, clean with physiological saline, and with after filter paper suck dry moisture, accurately taking liver 100mg, hepatic glycogen detection kit detects Mouse Liver glycogen content.
Take a blood sample after the mensuration last administration 30min of 6.4 blood lactase acid, then do not bear a heavy burden stops after temperature is the water went swimming 10min of 30 DEG C.Lactic acid instrument assay method: after rest 20min, each blood sampling 20 μ L add in 40 μ L rupture of membranes liquid before swimming, after swimming, after swimming respectively, the smudge cells lactic acid instrument that fully vibrates immediately measures.(blood lactase acid TG-AUC=5 × (the blood lactase acid value of the rear 20min of blood lactase acid value+2 × swimming of the rear 0min of front blood lactase acid value+3 × swimming that swims)
7. observation index walking weight load, blood lactase acid, urea, glycogen initial value
8. statistical method experimental data is used
represent, employing t inspection is compared between organizing
9. experimental result
9.1 present compositions are on the impact of mice burden swimming time
G1-G23 sample compares with blank group, can obviously extend the mice burden swimming time, there is significant difference (P < 0.05), G24-G26 sample compares with blank group, can the significant prolongation mice burden swimming time, there is pole significant difference (P < 0.01), and be obviously better than G1-G23 sample.The results detailed in Table 3.
The impact of table 3 agate coffee of the present invention Effects of Pollen in Mice walking weight load
| Experimental group | Dosage (g/kg) | Number of animals (only) | Walking weight load (min) |
| Blank | 1 | 10 | 3.23±0.79 |
| G1 | 1 | 10 | 4.54±1.78* |
| G2 | 1 | 10 | 4.88±1.23* |
| G3 | 1 | 10 | 4.65±1.34* |
| G4 | 1 | 10 | 4.34±1.56* |
| G5 | 1 | 10 | 4.78±1.67* |
| G6 | 1 | 10 | 4.96±1.78* |
| G7 | 1 | 10 | 4.57±1.56* |
| G8 | 1 | 10 | 4.78±1.67* |
| G9 | 1 | 10 | 4.97±1.75* |
| G10 | 1 | 10 | 4.67±1.83* |
| G11 | 1 | 10 | 4.79±1.85* |
| G12 | 1 | 10 | 4.76±1.64* |
| G13 | 1 | 10 | 4.67±1.53* |
| G14 | 1 | 10 | 4.86±1.75* |
| G15 | 1 | 10 | 4.78±1.67* |
| G16 | 1 | 10 | 4.67±3.45* |
| G17 | 1 | 10 | 4.78±3.64* |
| G18 | 1 | 10 | 4.85±3.26* |
| G19 | 1 | 10 | 4.95±3.45* |
| G20 | 1 | 10 | 4.79±3.17* |
| G21 | 1 | 10 | 5.10.±0.66* |
| G22 | 1 | 10 | 4.99±1.25* |
| G23 | 1 | 10 | 5.09±1.86* |
| G24 | 1 | 10 | 6.35±3.23** |
| G25 | 1 | 10 | 6.01±1.26** |
| G26 | 1 | 10 | 6.13±1.55** |
" * " p<0.05vs blank; " * * " p<0.01vs blank;
9.2 present compositions are on the impact of blood lactase acid before and after mouse movement
After per os gives mouse agate coffee of the present invention pollen, G24-G26 sample to compare with control group blood lactase acid TG-AUC after mouse movement significant difference (P < 0.05), decrease though G1-G23 sample sets Mouse Blood lactic acid TG-AUC compares with control group, and no difference of science of statistics (P > 0.05).The results are shown in Table 4.
The impact of blood lactase acid level before and after the motion of table 4 agate coffee of the present invention Effects of Pollen in Mice
| Experimental group | Dosage (g/kg) | Number of animals (only) | Blood lactase acid TG-AUC (mmol/L) |
| Blank | 1 | 10 | 363.8±21.7 |
| G1 | 1 | 10 | 326.5±23.0 |
| G2 | 1 | 10 | 325.3±17.4 |
| G3 | 1 | 10 | 327.4±31.3 |
| G4 | 1 | 10 | 328.1±22.6 |
| G5 | 1 | 10 | 325.6±35.6 |
| G6 | 1 | 10 | 323.4±33.5 |
| G7 | 1 | 10 | 330.9±33.3 |
| G8 | 1 | 10 | 323.1±37.3 |
| G9 | 1 | 10 | 322.2±38.1 |
| G10 | 1 | 10 | 326.0±34.2 |
| G11 | 1 | 10 | 322.6±36.4 |
| G12 | 1 | 10 | 321.8±33.3 |
| G13 | 1 | 10 | 321.4±34.5 |
| G14 | 1 | 10 | 322.3±32.7 |
| G15 | 1 | 10 | 324.5±31.5 |
| G16 | 1 | 10 | 322.3±29.9 |
| G17 | 1 | 10 | 326.7±26.6 |
| G18 | 1 | 10 | 327.9±27.7 |
| G19 | 1 | 10 | 324.3±22.4 |
| G20 | 1 | 10 | 326.8±28.7 |
| G21 | 1 | 10 | 325.3±31.9 |
| G22 | 1 | 10 | 328.4±29.3 |
| G23 | 1 | 10 | 326.1±28.7 |
| G24 | 1 | 10 | 299.6±28.7* |
| G25 | 1 | 10 | 303.4±28.7* |
| G26 | 1 | 10 | 302.3±28.7* |
" * " p<0.05vs blank;
The impact of 9.3 agate coffee Effects of Pollen in Mice hepatic glycogen of the present invention
After per os gives mouse samples, G1-G23 sample compares with blank group, Mouse Liver glycogen content all has obvious rising, there is significant difference (P < 0.05), G24-G26 sample compares with blank group, Mouse Liver glycogen content all has obvious rising, has pole significant difference (P < 0.01), and is obviously better than G1-G23 sample.The results detailed in Table 5.
The impact of table 5 agate coffee of the present invention Effects of Pollen in Mice hepatic glycogen content
| Experimental group | Dosage (g/kg) | Number of animals (only) | Hepatic glycogen (g/100g hepatic tissue) |
| Blank | 1 | 10 | 8.6±1.1 |
| G1 | 1 | 10 | 10.9±3.2* |
| G2 | 1 | 10 | 10.6±3.3* |
| G3 | 1 | 10 | 10.5±4.4* |
| G4 | 1 | 10 | 10.2±2.5* |
| G5 | 1 | 10 | 10.1±3.6* |
| G6 | 1 | 10 | 10.6±3.4* |
| G7 | 1 | 10 | 10.4±3.6* |
| G8 | 1 | 10 | 10.1±2.8* |
| G9 | 1 | 10 | 10.5±2.3* |
| G10 | 1 | 10 | 10.3±3.9* |
| G11 | 1 | 10 | 10.2±3.6* |
| G12 | 1 | 10 | 9.8±3.7* |
| G13 | 1 | 10 | 10.2±3.5* |
| G14 | 1 | 10 | 10.1±3.5* |
| G15 | 1 | 10 | 10.5±3.6* |
| G16 | 1 | 10 | 10.1±2.5* |
| G17 | 1 | 10 | 10.6±2.8* |
| G18 | 1 | 10 | 10.7±3.1* |
| G19 | 1 | 10 | 10.8±3.2* |
| G20 | 1 | 10 | 10.9±2.9* |
| G21 | 1 | 10 | 11.0±3.1* |
| G22 | 1 | 10 | 11.2±1.6* |
| G23 | 1 | 10 | 11.1±3.4* |
| G24 | 1 | 10 | 13.6±3.9** |
| G25 | 1 | 10 | 13.0±2.1** |
| G26 | 1 | 10 | 13.1±3.6** |
" * " p<0.05vs blank; " * * " p<0.01vs blank;
The impact of 9.4 agate coffee Effects of Pollen in Mice serum ureas of the present invention
After per os gives mouse samples, G1-G23 sample sets compares with blank group, after mouse movement, serum urea content all has obvious reduction, there is significant difference (P < 0.05), agate coffee pollen G24-G26 sample of the present invention compares with blank group, after mouse movement, serum urea content all has obvious reduction, has pole significant difference (P < 0.01), and is obviously better than G1-G23 sample.The results detailed in Table 6
The impact of table 6 agate coffee of the present invention Effects of Pollen in Mice serum urea content
| Experimental group | Dosage (g/kg) | Number of animals (only) | Serum urea (mmol/L) |
| Blank | 1 | 10 | 10.6±1.1 |
| G1 | 1 | 10 | 7.4±1.5* |
| G2 | 1 | 10 | 7.5±1.3* |
| G3 | 1 | 10 | 7.5±1.1* |
| G4 | 1 | 10 | 7.6±1.5* |
| G5 | 1 | 10 | 7.7±1.6* |
| G6 | 1 | 10 | 7.8±1.7* |
| G7 | 1 | 10 | 7.9±1.9* |
| G8 | 1 | 10 | 7.5±1.8* |
| G9 | 1 | 10 | 7.6±1.7* |
| G10 | 1 | 10 | 7.7±1.6* |
| G11 | 1 | 10 | 7.7±1.7* |
| G12 | 1 | 10 | 7.8±1.7* |
| G13 | 1 | 10 | 7.6±1.9* |
| G14 | 1 | 10 | 7.9±1.6* |
| G15 | 1 | 10 | 7.8±1.9* |
| G16 | 1 | 10 | 7.7±1.3* |
| G17 | 1 | 10 | 7.6±1.5* |
| G18 | 1 | 10 | 7.8±1.1* |
| G19 | 1 | 10 | 7.6±1.2* |
| G20 | 1 | 10 | 7.5±1.7* |
| G21 | 1 | 10 | 7.4±1.3* |
| G22 | 1 | 10 | 7.5±1.2* |
| G23 | 1 | 10 | 7.4±1.5* |
| G24 | 1 | 10 | 6.8±1.2** |
| G25 | 1 | 10 | 7.0±1.1** |
| G26 | 1 | 10 | 6.9±1.7** |
" * " p<0.05vs blank; " * * " p<0.01vs blank;
10. experiment conclusion
Test mainly through mice burden swimming experiment, detect the index such as deposit, mice serum urea content of Mouse Liver glycogen simultaneously, observe the antifatigue effect of agate coffee pollen of the present invention.Preliminary Results shows below:
1, the embodiment of the present invention 1,2,3 agate coffee pollen be also G24-G26 group all energy significant prolongation mice burden swimming time (P < 0.01), and successful is better than other groups.
2, biochemistry detection aspect display, the lactic acid content that after G24-G26 group of the present invention all can reduce motion, in mice serum, glucose anerobic glycolysis produces, compare with control group and have significant difference (P < 0.05), and although other groups also can reduce the lactic acid content that in the rear mice serum of motion, glucose anerobic glycolysis produces, but compare with control group, no difference of science of statistics (P > 0.05);
3, G24-G26 group of the present invention all can significantly improve the deposit (P < 0.01) of glycogen in mouse liver, and successful is better than other groups;
4, G24-G26 group of the present invention significantly can reduce the content (P < 0.01) of the rear urea in serum of mouse swimming, and successful is better than other groups;
11. discuss
Above-mentioned experiment proves that agate coffee pollen of the present invention significantly can reduce the content of urea in serum and lactic acid after mouse movement, and the deposit of glycogen in mouse liver can be significantly improved, point out it can reduce the loss of energy by the metabolism of two large main energetic matter protein and glucose in minimizing energetic supersession, contribute to the fatigue that alleviation exercise load causes; The time that mice burden swimming to power exhausts can be extended.
Also illustrate simultaneously by agate coffee according to the present invention fill a prescription composite after, each substance reaches the effect of Synergistic, and the health-care effects such as antifatigue have obviously surmounted the effect that each composition is used alone or arbitrarily combines.
Claims (10)
1. an agate coffee pollen, comprises the raw material composition of following parts by weight: rape pollen 20-30 part, pueraria root powder 40-60 part, mushroom powdered beef 1-10 part, yam flour 1-5 part, mango powder 1-10 part, pumpkin seed powder 6-10 part, isomalt 2-10 part, composite dietary 1-6 part.
2. agate coffee pollen according to claim 1, is characterized in that, described pueraria root powder is prepared by the method comprised the steps:
After agate coffee impurity elimination cleaning, 5 times of soft water are added by agate coffee weight, after corase grind grinding, add the soft water of agate coffee weight 8 times, and add pumpkin seed powder, addition is the 1-2% of agate coffee weight, grind through colloid mill after mixing, slurries add mixed enzyme and carry out enzymolysis, pH5.2-5.5, temperature 45-50 DEG C, enzymolysis time 3-4hr, described mixed enzyme consumption is the 0.05-0.2% of slurry weight, and described mixed enzyme parts by weight consist of: pectase 4 parts, 1,4 beta-glucanase 1 part, 2 parts, protease and cellulase 2 parts; Enzymolysis liquid goes out enzyme, filtration, pulverizes and get final product after freeze concentration, freeze drying.
3. agate coffee pollen according to claim 1, it is characterized in that, described composite dietary comprises the composition of following parts by weight: xylo-oligosaccharide 1 part, Semen Tritici aestivi fiber 1.5 parts, fibre and soya 0.5 part.
4. agate coffee pollen according to claim 1, is characterized in that, described mushroom powdered beef is prepared by the following method:
Mushroom process: select dried thin mushroom, cleans up, and 2-3 times of 60-70 DEG C of emerge in worm water 20-40min, is cooled to normal temperature rapidly, and after Separation of Solid and Liquid, bubble mushroom water retains for subsequent use;
-1-1 DEG C of degree is refrigerated to after beef cleaning, rub together with mushroom, add the water of mixed material 1-2 times weight, comprising described bubble mushroom water, the gap of adjustment colloid mill stator and rotor is 60 ~ 120 microns, colloid mill flow-control is 0.1 ~ 0.3 ton/hour and makes mixing gravy, adjustment pH is 8-10, add the trypsase of gravy weight 0.1-0.25% and the alkali protease of 0.05-0.1%, 37-45 DEG C of mixed enzymolysis 1-2h, adjust pH to 5-6.5, be warming up to 50-58 DEG C, add the compound fertilizer production enzymolysis 3-4h of gravy weight 0.2-0.3%, go out enzyme, homogeneous, concentrated, crushed after being dried and get final product,
The weight ratio of described beef and mushroom is: beef: mushroom=10-15:1-3.
5. agate coffee pollen according to claim 1, it is characterized in that, described yam flour is prepared by the following method:
Chinese yam is removed the peel, weigh, stripping and slicing, defibrination, add 18-20 times of soft water according to peeling Chinese yam weight and carry out the extraction of microwave cooperating ultrasonic wave, microwave power is 500-800W, frequency 2450MHz; Ultrasonic power 800-1000W, frequency 40KHz, the time is 15-45min; It is 80% that extract adds absolute ethyl alcohol to solution alcohol concentration, filters after alcohol precipitation 14hr; Filter residue is dissolved in 3-4 times of pure water, Vacuum Concentration, and vacuum drying lower than 5%, crosses 80-100 mesh sieve after pulverizing to moisture.
6. agate coffee pollen according to claim 1, it is characterized in that, described mango powder is prepared by the following method:
Mango cleaning peeling stoning, after adding pulp weight 6-12 colour protecting liquid making beating doubly, the pH value controlling solution is 4-5, add with the complex enzyme of pulp weighing scale 0.1-0.5%, described complex enzyme is made up of acid pectase, neutral proteinase and cellulase, the weight ratio of three kinds of enzymes is 2.5: 1.5: 1, and hydrolysis temperature is 50-53 DEG C, and enzymolysis time is 1.5-3hr; Afterwards pH value is adjusted to 5.1-5.6, temperature is raised to 55-60 DEG C, adds with the dextranase of the pectase of pulp weighing scale 0.2-0.5%, 0.2-0.6% simultaneously, and action time is 2.5-3hr; Afterwards by enzymolysis liquid under 0.6A/25W-1.5A/275W condition, ultrasonic extraction 20-30min, extract is 5-10 DEG C of filtration; Filtrate freeze concentration, freeze drying, pulverizes and obtains mango powder;
The aqueous ascorbic acid of described colour protecting liquid to be pH be 0.15% of 6.5-6.8.
7. agate coffee pollen according to claim 6, is characterized in that, described filtration, and medium is the mixture of diatomite and hickory chick powder, and in volume of material to be filtered, food grade kieselguhr use amount is: 10-50g/L, and hickory chick powder use amount is 0.1-0.2g/L.
8. agate coffee pollen according to claim 1, is characterized in that, described pumpkin seed powder is that after pumpkin seeds impurity elimination, belt leather is broken through air blast ultramicro powder, makes fineness reach 2-10 micron and obtains.
9. the preparation method of agate coffee pollen according to claim 1-8, comprise the steps: by each raw material of formula precise, mix after rape pollen ultramicro grinding with pueraria root powder, and add pumpkin seed powder, mushroom powdered beef, yam flour, mango powder, composite dietary successively, finally add the rear ultramicro grinding of isomalt mixing, make fineness reach 2-10 micron.
10. the preparation method of agate coffee pollen according to claim 9, is characterized in that, described pueraria root powder preparation method comprises the steps:
After agate coffee impurity elimination cleaning, add 5 times of soft water by agate coffee weight, after corase grind grinding, add the soft water of agate coffee weight 8 times, and adding pumpkin seed powder, addition counts 2% with agate coffee weight, grinds after mixing through colloid mill, slurries add mixed enzyme and carry out enzymolysis, pH5.3, temperature 46 DEG C, enzymolysis time 3.5hr, described mixed enzyme consumption is 0.1% of slurry weight, and described mixed enzyme parts by weight consist of: pectase 4 parts, 1,4 beta-glucanase 1 part, 2 parts, protease and cellulase 2 parts; Enzymolysis liquid goes out enzyme, filtration, pulverizes and get final product after freeze concentration, freeze drying;
Described composite dietary comprises the composition of following parts by weight: xylo-oligosaccharide 1 part, Semen Tritici aestivi fiber 1.5 parts, fibre and soya 0.5 part;
Described mushroom powdered beef preparation method comprises the steps:
Mushroom process: select dried thin mushroom, cleans up, and 3 times of 65 DEG C of emerge in worm water 30min, are cooled to normal temperature rapidly, and bubble mushroom water retains for subsequent use;
-1 DEG C of degree is refrigerated to after beef cleaning, rub together with mushroom, add the water of mixed material 2 times of weight, comprising described bubble mushroom water, the gap of adjustment colloid mill stator and rotor is 100 microns, colloid mill flow-control is 0.2 ton/hour and makes mixing gravy, and adjustment pH is 9, adds the trypsase of gravy weight 0.1% and the alkali protease of 0.1%, 41 DEG C of mixed enzymolysis 2h, adjust pH to 6.0, be warming up to 54 DEG C, add the compound fertilizer production enzymolysis 3h of gravy weight 0.3%, go out enzyme, homogeneous, concentrated, crushed after being dried and get final product;
The weight ratio of described beef and mushroom is: beef: mushroom=10:3;
Described yam flour preparation method comprises the steps:
Chinese yam is removed the peel, weigh, stripping and slicing, defibrination, add 18 times of soft water according to peeling Chinese yam weight and carry out the extraction of microwave cooperating ultrasonic wave, microwave power is 650W, frequency 2450MHz; Ultrasonic power 900W, frequency 40KHz, the time is 30min; It is 80% that extract adds absolute ethyl alcohol to solution alcohol concentration, filters after alcohol precipitation 14hr; Filter residue is dissolved in 4 times of pure water, Vacuum Concentration, and vacuum drying lower than 5%, crosses 80 mesh sieves after pulverizing to moisture;
Described method for preparation of mango powder comprises the steps:
Mango cleaning peeling stoning, after adding the colour protecting liquid making beating of pulp weight 8 times, the pH value controlling solution is 4.5, add with the complex enzyme of pulp weighing scale 0.3%, described complex enzyme is made up of acid pectase, neutral proteinase and cellulase, the weight ratio of three kinds of enzymes is 2.5: 1.5: 1, and hydrolysis temperature is 50 DEG C, and enzymolysis time is 3hr; Afterwards pH value is adjusted to 5.1, temperature is raised to 55 DEG C, add simultaneously with the pectase of pulp weighing scale 0.5%, 0.2% dextranase, action time is 3hr; Afterwards by enzymolysis liquid under 1.5A/275W condition, ultrasonic extraction 20min, extract is 5 DEG C of filtrations; Filtrate freeze concentration, freeze drying, pulverizes and obtains mango powder;
The aqueous ascorbic acid of described colour protecting liquid to be pH be 0.15% of 6.5-6.8;
Described filtration, medium is the mixture of diatomite and hickory chick powder, and in volume of material to be filtered, food grade kieselguhr use amount is: 30g/L, and hickory chick powder use amount is 0.1g/L;
Described pumpkin seed powder is that after pumpkin seeds impurity elimination, belt leather is broken through air blast ultramicro powder, makes fineness reach 2-10 micron and get final product;
Described agate coffee pollen is made up of the raw material of following parts by weight: rape pollen 25 parts, pueraria root powder 60 parts, mushroom powdered beef 5 parts, yam flour 3 parts, mango powder 6 parts, pumpkin seed powder 8 parts, isomalt 6 parts, composite dietary 4 parts.
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