CN107574258A - With the soft thorn gene Ts of the cucumber fruits InDel Ts2 molecular labelings isolated and its application - Google Patents
With the soft thorn gene Ts of the cucumber fruits InDel Ts2 molecular labelings isolated and its application Download PDFInfo
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- CN107574258A CN107574258A CN201710861459.4A CN201710861459A CN107574258A CN 107574258 A CN107574258 A CN 107574258A CN 201710861459 A CN201710861459 A CN 201710861459A CN 107574258 A CN107574258 A CN 107574258A
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Abstract
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Claims (7)
- A kind of 1. InDel-Ts2 molecular labelings isolated with the soft thorn gene Ts of cucumber fruits, it is characterised in that the molecular labeling With 107 nucleotide sequences shown in sequence table SEQ ID NO.1, and 105 cores shown in sequence table SEQ ID NO.2 Nucleotide sequence in nucleotide sequence, wherein SEQ ID NO.1 isolates with soft thorn gene, the nucleotides in SEQ ID NO.2 Sequence isolates with hard Ci Jiyin.
- 2. a kind of InDel-Ts2 molecular labelings isolated with the soft thorn gene Ts of cucumber fruits according to claim 1, its It is characterised by, the molecular labeling is expanded to obtain as the anti-sense primer shown in the sense primer and EM6 shown in ME5,The primer ME5 sequences, it is specially:ME5:5 '-GGTTGAAGGTTGATTGGA-3 ',The primer EM6 sequences, it is specially:EM6:5’-CTCATAACGTCATCCCTAAA-3’.
- A kind of 3. preparation method of the InDel-Ts2 molecular labelings isolated with the soft thorn gene Ts of cucumber fruits, it is characterised in that The molecular labeling is expanded to obtain as the anti-sense primer shown in the sense primer and EM6 shown in ME5,The primer ME5 sequences, it is specially:ME5:5 '-GGTTGAAGGTTGATTGGA-3 ',The primer EM6 sequences, it is specially:EM6:5’-CTCATAACGTCATCCCTAAA-3’.
- 4. a kind of InDel-Ts2 molecular labelings isolated with the soft thorn gene Ts of cucumber fruits obtain according to claim 3 Method, it is characterised in that reaction system is genomic DNA 30ng, primer 0.5 μm of ol/L, 200 μm of ol/L dNTPs, 2mmol/L MgCl2, 1 μ l 10 × PCR reactions buffer, 0.6U Taq DNA polymerases, overall reaction system is 10 μ L, primer are anti-sense primer shown in sense primer shown in ME5 and EM6.
- 5. a kind of InDel-Ts2 molecular labelings isolated with the soft thorn gene Ts of cucumber fruits obtain according to claim 3 Method, it is characterised in that PCR amplification programs are:94 DEG C of 5min, 94 DEG C of 25s, 55 DEG C of 25s, 72 DEG C of 25s, 32 circulations, expand Volume increase thing is separated with 8% native polyacrylamide gel electrophoresis, and electrophoretic buffer is 0.5 × TBE, 160W invariable powers, electrophoresis 3h。
- 6. as claimed in claim 1 with the application of the soft thorn gene Ts of the cucumber fruits InDel-Ts2 molecular labelings isolated, it is special Sign is, the molecular labeling is used for various cucumber and pierces/the screening of soft thorn type firmly.
- 7. according to claim 6 with the application of the soft thorn gene Ts of the cucumber fruits InDel-Ts2 molecular labelings isolated, its It is characterised by, the molecular labeling, which is used for the early stage that during vegetable seeds or cotyledon is grown, to be identified.
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109182588A (en) * | 2018-10-31 | 2019-01-11 | 上海交通大学 | The molecular labeling Indel-de2 isolated with cucumber one dimensional infinite wall gene |
CN109234439A (en) * | 2018-10-31 | 2019-01-18 | 上海交通大学 | The molecular labeling Indel-de1 isolated with cucumber determinate growth gene |
CN109234438A (en) * | 2018-10-31 | 2019-01-18 | 上海交通大学 | The molecular labeling Indel-De3 isolated with cucumber one dimensional infinite wall gene |
Citations (3)
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CN101250523A (en) * | 2008-04-03 | 2008-08-27 | 上海交通大学 | Molecule marker tightly linked with cucumber pubescence gene G1 |
CN106939351A (en) * | 2017-05-03 | 2017-07-11 | 江苏师范大学 | Dominant molecular labeling of the quick screening cucumber with/without fruit knurl character |
CN106939352A (en) * | 2017-05-03 | 2017-07-11 | 江苏师范大学 | Quick screening cucumber pierces firmly/the InDel molecular labelings of soft bur real type |
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2017
- 2017-09-21 CN CN201710861459.4A patent/CN107574258A/en active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101250523A (en) * | 2008-04-03 | 2008-08-27 | 上海交通大学 | Molecule marker tightly linked with cucumber pubescence gene G1 |
CN106939351A (en) * | 2017-05-03 | 2017-07-11 | 江苏师范大学 | Dominant molecular labeling of the quick screening cucumber with/without fruit knurl character |
CN106939352A (en) * | 2017-05-03 | 2017-07-11 | 江苏师范大学 | Quick screening cucumber pierces firmly/the InDel molecular labelings of soft bur real type |
Non-Patent Citations (2)
Title |
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QIANG LI等: "The identification of cucumis sativus glabrous 1(CSGL1) required for the formation of trichomes uncovers a novel function for the homeodomain-leucine zipper I gene", 《JOURNAL OF EXPERIMENTAL BOTANY》 * |
XU MENGNAN等: "A new gene conferring the glabrous trait in cucumber identified using mutmap", 《HORTICULTURAL PLANT JOURNAL》 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109182588A (en) * | 2018-10-31 | 2019-01-11 | 上海交通大学 | The molecular labeling Indel-de2 isolated with cucumber one dimensional infinite wall gene |
CN109234439A (en) * | 2018-10-31 | 2019-01-18 | 上海交通大学 | The molecular labeling Indel-de1 isolated with cucumber determinate growth gene |
CN109234438A (en) * | 2018-10-31 | 2019-01-18 | 上海交通大学 | The molecular labeling Indel-De3 isolated with cucumber one dimensional infinite wall gene |
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Inventor after: Pan Junsong Inventor after: Lv Duo Inventor after: Wang Gang Inventor after: Cai Run Inventor after: Zhu Wenying Inventor after: Xiao Tingting Inventor after: Sun Jingxian Inventor after: Guo Chunli Inventor before: Pan Junsong Inventor before: Wang Gang Inventor before: Cai Run Inventor before: Zhu Wenying Inventor before: Xiao Tingting Inventor before: Sun Jingxian Inventor before: Guo Chunli |
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