CN107568466A - A kind of milk cow tryptophan feed, feed manufacture method and tryptophan application - Google Patents
A kind of milk cow tryptophan feed, feed manufacture method and tryptophan application Download PDFInfo
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- CN107568466A CN107568466A CN201711058791.3A CN201711058791A CN107568466A CN 107568466 A CN107568466 A CN 107568466A CN 201711058791 A CN201711058791 A CN 201711058791A CN 107568466 A CN107568466 A CN 107568466A
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Abstract
The application for improving milk cow cow's milk melatonin levels is used as the present invention relates to a kind of milk cow tryptophan feed, the manufacture method of feed, the method for the melatonin levels of raising cow's milk and tryptophan.The principle of the present invention is：Because tryptophan is the precursor substance for synthesizing melatonin, the tryptophan to dissociate in blood can enter brain by blood-brain barrier, be changed into melatonin in the presence of enzyme.
Description
Technical field
The present invention relates to a kind of milk cow tryptophan feed, the manufacture method of feed, the melatonin levels of raising cow's milk
Method and tryptophan as improve milk cow cow's milk melatonin levels application.
Background technology
China belongs to sleep-disorder or insomnia country occurred frequently, and as people's rhythm of life is accelerated, diet is irregular, overtime work is endured
Night, electromagnetic environment and the factors such as stress is big cause the insomnia incidence of disease to steeply rise.Insomnia seriously endanger human health and
Life, its aging rate of the patient often having a sleepless night are 3 times of normal person.Chinese Medical Association's data shows that China there are about 300,000,000 adults
People is with sleep-disorder such as insomnias.With age, the sleep of the mankind 1 day tapers off trend total time, insomnia of the old people morbidity
Rate is up to 70%.Research confirms：Melatonin hyposecretion is the main reason for causing different degrees of sleep-disorder and insomnia
One of.
At present in world wide, only a few countries such as U.S. and China and area using artificial synthesized melatonin as
Artificial synthesized melatonin is classified as medicine by the overwhelming majority of countries such as food additives, Canada, Britain and France, it is impossible to is made
For food additives.Therefore, research and development turn into rich in natural melatonin food advocates natural, the safe crowd of pursuit compel in the world
It is essential and asks.
Milk is the most dairy products of mass consumption, containing a certain amount of natural melatonin, with it is artificial synthesized take off it is black
Hormone is compared, and natural or endogenous melatonin has high security.Milk is that the good complement of exogenous melatonin is come
Source, and melatonin levels are high compared with daytime in night milk, therefore some dairy enterprises are rich in height by light-operated research and development in the world
The milk of natural melatonin.Finland's Ying Geman dairy industries (Ingman Dairy) in 1999 and the endocrinology of Kuopio university
Natural high melatonin milk is successfully developed in professor's cooperation, and the solid growth for realizing sale improves the added value of milk.With
Afterwards, the state such as Japan, UK ＆ Ireland has natural high melatonin dairy product to come out in succession, and price is the 2.5 of ordinary milk
Times, quite get consumer reception.Chinese scholar in 2012 has invented a kind of high original of acquisition melatonin content also by light control techniques
The method of material milk, but have no that corresponding product comes out in the domestic market so far.
A kind of production method rich in natural melatonin milk has been recorded in Finnish patent PCT/FI2000/000589.This
In method, the length in dark cycle and photoperiod in milk cow 1d is basically unchanged, and time span is respectively 2-11h and 13-22h.
In milking operation, the maximum intensity of illumination of milk cow eye contact is 40lx.23 in cow house:00 to next day 07:00 illumination
Intensity is maintained at 10lx, and morning, milking work was 06:Completed before 30, according to normal procedure to milk cow milking in other times
Obtain ordinary milk.Light regime is standardized, it is ensured that can obtain melatonin levels in Various Seasonal and exist
More than 20pg/mL high melatonin milk.In cattle farm it was verified that in intensity of illumination under the same conditions, using the method
The light regime (illumination/dark is 17/7) of recommendation takes off compared with light regime (illumination/dark is 12/12) before in milk
The content of black hormone increases to 56.4pg/mL by 8.9pg/mL.
In addition, a kind of described in BP UK20020005211.6 improve natural hormone content method in milk.This is special
Sharp method is needed to control intensity of illumination and light application time, and illumination is divided into dark cycle and photoperiod, while needs to increase animal
Dry matter intake.Optimal dark cycle time is 8-10h, photoperiod 14-16h.The luminous intensity in dark cycle is not to be exceeded
50lx, the average luminous intensity of photoperiod should at least 1 000lx., can be about 19 if only carrying out 1 non-dark milking:
00 is milked, and dark milking is about 05：00 is carried out；If 3 milkings in 24h, Milking time is respectively 05：00、13:00 He
21:00.The content of melatonin is 15-30pg/mL in the milk that the method provided using this patent produces.
In addition, the Chinese invention patent that notification number is CN101791006B describes a kind of high melatonin levels of acquisition
The method of raw milk, the method that this patent obtains high melatonin breast are as follows：The raising dairy cattle of lactation period is according to intensity of illumination point
For illumination period, dark period and adopt the milk phase.Illumination period 03:00-15:00, intensity of illumination 540-600lx；Dark period 15:00 to secondary
Day 02:30, dark period is every 15~30min of blue green light illumination that 1-2h wavelength is 420-480nm, blue green light intensity of illumination
240-300lx, the control of remaining time intensity of illumination is 20-40lx outside dark period blue green light illumination；Adopt the milk phase 02:30-03:
00, adopt the milk phase is illuminated with the blue green light that wavelength is 420-480nm, intensity of illumination is 240-300lx.Use this patented method
The content of melatonin is 50-71pg/mL in the raw milk of production, has obtained the raw milk of high melatonin content.
Above patent of invention is that the side of high melatonin raw milk is obtained by controlling light application time and illumination length
Method, although melatonin content is higher in raw milk obtained by these methods, there is also many in actual production for this method
Drawback, cowshed need to accordingly be transformed as changed periodicity of illumination and intensity of illumination in actual production, cause cost to increase；Need to be
Night is milked, and is unfavorable for cow's welfare；Night no light is unfavorable for milk cow and searched for food at night, causes output of milk decline or profit
It is more low with lactose content in the raw milk obtained by control illumination.
The content of the invention
The drawbacks of in order to overcome the method for the above-described high epiphysin milk of production, a kind of addition of present invention offer are taken off black
The mode of hormone precursor material tryptophan is to obtain the method for pure natural high melatonin raw material milk.
Present invention firstly provides a kind of milk cow tryptophan feed, tryptophan feed is rumen-protected property tryptophan.
A kind of manufacture method present invention also offers milk cow with tryptophan feed, it is coated with step and stability test step
Suddenly：Tryptophan is coated with to obtain rumen-protected property tryptophan, determines the small intestine disappearance rate of rumen-protected property tryptophan.
One preferable scheme is, in stability test step：Artificial saliva digestion trial, using half internal Nylon Bag
Method determines degradation rate in cud of rumen-protected property tryptophan, determines rumen-protected property using improved three steps in vitro method
The small intestine disappearance rate of tryptophan.
Present invention also offers a kind of method for the melatonin levels for improving cow's milk：This method includes following step：
S1：Obtain rumen-protected property tryptophan；
S2：Maize flour plus water stirring are made thick, then rumen-protected property tryptophan is spread to sticky jade
On rice and flour, together feed to milk cow；
S3：Milk cow discharges the cow's milk containing melatonin.
One preferable scheme is that rumen-protected property tryptophan addition is 28.0g/ days/head.
Present invention also offers a kind of tryptophan as the application for improving milk cow cow's milk melatonin levels.
One preferable scheme is that tryptophan is rumen-protected property tryptophan.
One preferable scheme is that tryptophan improves the application of milk cow cow's milk melatonin levels as milk cow forage.
The principle of the present invention is：Because tryptophan is the precursor substance for synthesizing melatonin, the color ammonia to dissociate in blood
Acid can enter brain by blood-brain barrier, be changed into melatonin in the presence of enzyme.After adding rumen-protected tryptophan
Melatonin concentration dramatically increases in milk cow in lactation period serum and breast.Melatonin levels are increased to by 20pg/ml in serum
51pg/mL；Melatonin concentration increases to 53.2pg/ml by 11.3pg/ml in breast
Milk cow is compared as ruminant with nonruminant special alimentary canal structure and digestive physiology, and it has complex stomach
Structure, i.e. cud, reticulum, omasum and abomasum, its neutralize nonruminant main difference is that cud, cud do not have glandula digestive, phase
When in a live body anaerobic fermentation tank, the protein in feed and energy are converted into microprotein and volatile fatty acid.
Protein in feed is divided into rumen-undegraded protein and rumen degradable protein matter two parts, the micro- life of cud after entering cud
Thing can utilize rumen degradable protein synthetic animalcule albumen, the non-degradable albumen of microprotein, cud and intrinsic protein three
Small intestine is diverted into, can be by intestinal absorption.Therefore, the present invention tryptophan feed making into rumen-protected property tryptophan.
Embodiment
A kind of milk cow tryptophan feed is present embodiments provided, wherein, tryptophan feed is rumen-protected property color ammonia
Acid.
The milk cow of the present embodiment is coated with step and stability test step with the manufacture method of tryptophan feed, wherein, it is right
Tryptophan is coated with to obtain rumen-protected property tryptophan, determines the small intestine disappearance rate of rumen-protected property tryptophan.Also,
Preferably in stability test step：Artificial saliva digestion trial, using half in vivo Nylon Bag determine rumen-protected property
Degradation rate of the tryptophan in cud, the small intestine disappearance using the rumen-protected property tryptophan of improved three steps in vitro method measure
Rate.Rumen-protected is to be protected gal4 amino acid or fat by technical finesse, avoids its quilt in cud
Fermentation, degraded, are allowed to be directly entered small intestine and are digested utilization.
The rumen-protected property tryptophan that the present embodiment provides is as the application for improving milk cow cow's milk melatonin levels.It is logical
The method for breeding for crossing change milk cow obtains the cow's milk of milk cow secretion.Specifically, the method bag of the melatonin levels of cow's milk is improved
Include following step：
S1：Rumen-protected property tryptophan is obtained, rumen-protected property tryptophan can be obtained from the prior art, such as
《Feed and herding》01 phase in 2010 discloses a kind of ruminally-protected tryptophan to mutton sheep nitrogen metabolism and the shadow of growth performance
Ring.
S2：Maize flour plus water stirring are made thick, then rumen-protected property tryptophan is spread to sticky jade
On rice and flour, together feed to milk cow.Specific adding method is as follows：First water stirring is added to make sticky fraction of maize flour
Shape, then the rumen-protected property tryptophan product weighed up is spread on sticky maize flour, together feed and give experiment milk cow, this side
Method can avoid rumen-protected property tryptophan product from being caused damage during feeding, ensure that every cow head can search for food
To enough rumen-protected property tryptophan products.
S3：Milk cow discharges the cow's milk containing melatonin.Study daily ration and add rumen-protected property tryptophan product to milk
The influence of melatonin in bovine blood and breast.Conclusion shows：Add milk cow in lactation period serum and breast after rumen-protected tryptophan
Middle melatonin concentration dramatically increases.Melatonin levels increase to 51pg/mL by 20pg/ml in serum；Taken off in breast black sharp
Plain concentration increases to 53.2pg/ml by 11.3pg/ml.
In addition, the present embodiment has also done following experimental design and data statistics.
Experimental design：By Animal experiment, most suitable addition and the addition side of rumen-protected property tryptophan are determined
Method.By 36 cow heads according to the same or like principle of parity, lactation amount, 3 groups, every group 12 are randomly divided into.It is rumen-protected
Property tryptophan addition sets three levels, respectively 0,14.0,28.0g/d/ heads, " g/d/ heads " can also be expressed as g/ days/head.
That is the tryptophan amount that every ox reaches small intestine daily is respectively 5.4,10.8 and 16.2g/d, d can be understood as day or 24 hours.
The computational methods of rumen-protected property tryptophan addition are as shown in the table.The most suitable rumen bypass of milk cow is obtained by result of study
Protectiveness tryptophan addition is 28.0g/d.
The Rumen parameter and Effective degradability of the daily ration tryptophan of table 1 are as follows：
Note：Select 3 fistula milk cows be used as experimental animal, by Nylon Bag determine test daily ration rumen digestibility,
Wherein, a% represents fast degradation part of the tryptophan in cud in ruminally-protected tryptophan sample；B% represents that cud is protected
At a slow speed degraded part of the tryptophan in cud in shield property tryptophan sample；C is the degradation rate of b parts；ERD is ruminally-protected
Effective degradability of the property tryptophan product in cud.Assay method is as follows：
Degradation rate computation model：(the mathematical index model that Ф rskov and McDonald (1979) are proposed)
Y=a+b × (1-e-ct)
Y is the disappearance rate of tryptophan of the Nylon Bag in cud after holdup time t；A is fast degradation part；B is at a slow speed
Degraded part；C is the velocity constant of degraded part at a slow speed.
Effective degradabilities (Effective ruminal degradability, ERD) of the RPT in cud is by formula ERD
=a+ [bc/ (c+k)] is calculated.Constant k in formula is the cud outflow rate of testing sample, and crop residues cud is eaten
Rotten circulating rate constant uses Tamminga etc. theoretical value, i.e. protein feeds K is assumed to be 0.036/h.
The amount that table 2 reaches the tryptophan of small intestine after rumen bypass is as follows：
Note：Dry matter intake and tryptophan appetite are measured value.
The tryptophan (g/kg) of Rumen=1000g × tryptophane (%) × cud tryptophan degradation rate (%)；
The undegraded tryptophan of cud (g/kg)=1000g × tryptophane (%)-Rumen tryptophan (g/kg)；
Reach tryptophan (g/d)=dry matter intake (kg/d) × undegraded tryptophan of cud (g/kg) of small intestine.
Study daily ration and add influence of the rumen-protected property tryptophan product to dairy cattle and milk composition.Addition is not
Influence of the rumen-protected tryptophan of same amount (0g/d, 14.0g/d and 28.0g/d) to milk composition is as shown in table 3 below, draw as
Draw a conclusion：Daily ration adds rumen-protected property tryptophan to the dry matter intake and the output of milk of milk cow not only without influence, and
The milk composition of milk cow is significantly improved because have adjusted enteral amino acid balance, butterfat percnetage is brought up to from 3.17%
3.67%th, protein ratio brings up to 3.41% from 3.09%.
It is as follows that table 3 adds influence of the rumen-protected tryptophan to milk composition：
Obviously, described embodiment is only the part of the embodiment of the present invention, rather than whole embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art are obtained every other under the premise of creative work is not made
Embodiment, belong to the scope of protection of the invention.
Claims (8)
1. a kind of milk cow tryptophan feed, it is characterised in that the tryptophan feed is rumen-protected property tryptophan.
A kind of 2. milk cow manufacture method of tryptophan feed：The manufacture method includes following step：
It is coated with step：Tryptophan is coated with to obtain rumen-protected property tryptophan；
Stability test step：Determine the small intestine disappearance rate of rumen-protected property tryptophan.
3. the milk cow according to claim 2 manufacture method of tryptophan feed, it is characterised in that
In the stability test step：Artificial saliva digestion trial, using half in vivo Nylon Bag measure it is rumen-protected
Property degradation rate of the tryptophan in cud, the small intestine disappearance rate of the rumen-protected property tryptophan of measure.
A kind of 4. method for the melatonin levels for improving cow's milk：This method includes following step：
S1：Obtain rumen-protected property tryptophan；
S2：Maize flour plus water stirring are made thick, then rumen-protected property tryptophan is spread to sticky maize flour
On, together feed to milk cow；
S3：Milk cow discharges the cow's milk containing melatonin.
5. the method for the melatonin levels according to claim 4 for improving cow's milk：This method includes following step：
The rumen-protected property tryptophan addition is 28.0g/ days/head.
6. tryptophan is as the application for improving milk cow cow's milk melatonin levels.
7. tryptophan according to claim 6 is as the application for improving milk cow cow's milk melatonin levels, it is characterised in that
The tryptophan is rumen-protected property tryptophan.
8. tryptophan according to claim 6 is as the application for improving milk cow cow's milk melatonin levels, it is characterised in that
The tryptophan improves the application of milk cow cow's milk melatonin levels as milk cow forage.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115777624A (en) * | 2023-02-08 | 2023-03-14 | 北京三元食品股份有限公司 | Method for improving melatonin content in milk and application thereof |
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| CN101053372A (en) * | 2006-04-14 | 2007-10-17 | 中国农业大学 | Rumen bypass methionine feed for feeding cow and its preparation method and application |
| CN101664108A (en) * | 2009-09-21 | 2010-03-10 | 中国农业大学 | Rumen protected methionine and production method thereof |
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Patent Citations (3)
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|---|---|---|---|---|
| CN101053372A (en) * | 2006-04-14 | 2007-10-17 | 中国农业大学 | Rumen bypass methionine feed for feeding cow and its preparation method and application |
| CN101664108A (en) * | 2009-09-21 | 2010-03-10 | 中国农业大学 | Rumen protected methionine and production method thereof |
| CN104431369A (en) * | 2014-12-17 | 2015-03-25 | 宁夏伊品生物科技股份有限公司 | Rumen-protected lysine feed |
Non-Patent Citations (4)
| Title |
|---|
| M.T. KOLLMANN等: "Effects of tryptophan supplementation on plasma tryptophan and related hormone levels in heifers and dairy cows", 《DOMESTIC ANIMAL ENDOCRINOLOGY》 * |
| 国家科学研究委员会组织修订、孟庆翔主译: "《奶牛营养需要》", 31 August 2002, 中国农业大学出版社 * |
| 王加启: "高产奶牛饲料饲养研究新进展", 《中国乳业》 * |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115777624A (en) * | 2023-02-08 | 2023-03-14 | 北京三元食品股份有限公司 | Method for improving melatonin content in milk and application thereof |
| CN115777624B (en) * | 2023-02-08 | 2023-09-26 | 北京三元食品股份有限公司 | Method for increasing melatonin content in milk and application thereof |
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