CN107551866A - The mixing apparatus of adjuvant and antigen - Google Patents
The mixing apparatus of adjuvant and antigen Download PDFInfo
- Publication number
- CN107551866A CN107551866A CN201710765237.2A CN201710765237A CN107551866A CN 107551866 A CN107551866 A CN 107551866A CN 201710765237 A CN201710765237 A CN 201710765237A CN 107551866 A CN107551866 A CN 107551866A
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- vertical
- stirring cage
- central gear
- puddler
- syringe
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Mixers Of The Rotary Stirring Type (AREA)
- Mixers With Rotating Receptacles And Mixers With Vibration Mechanisms (AREA)
Abstract
The present invention relates to a kind of adjuvant and the mixing apparatus of antigen.To fill up prior art blank, the mixing apparatus of this adjuvant and antigen includes syringe, also include blender, blender includes open cup and mixing cap mechanisms, mixing cap mechanisms includes cover lid, upper stirring cage, lower stirring cage and fixed drive frame, a vertical pivot is fixed at cover lid center, upper stirring cage includes upper disk and a plurality of vertical puddler, a upper central gear is additionally provided with upper lower disk surface, lower stirring cage includes lower disc and multigroup vertical puddler, on, lower central gear projects coincidence in same level, fixed drive frame comprises at least two meteor gears, one radial level fixing axle and a motor, meteor gear simultaneously with it is upper, lower central gear engagement, one meteor gear is driven by motor, a fluid injection hole is provided with vertical pivot.It is design science of the present invention, simple, convenient laborsaving, it is applicable to close and prepares various Water-In-Oils, oil-water emulsifiers.
Description
Technical field
The present invention relates to a kind of adjuvant and the mixing apparatus of antigen.
Background technology
Freund's adjuvant is adjuvant the most frequently used in present zoopery, is divided into incomplete Freund's adjuvant and complete Freund assistant
Agent.Incomplete Freund's adjuvant is that atoleine mixes with lanolin, and component ratio is 1~5：1, can as needed depending on, lead to
It is often 2：1.
Add BCG vaccine (ultimate density is 3~20mg/ml) or dead mycobacterium tuberculosis, as Freund in Freund's incomplete adjuvant
Freund's complete adjuvant (CFA).Emulsified when typically injecting first with 1/2 volume FCA plus the antigen of 1/2 volume, second or the
Full adjuvant is cannotd be used up when injecting three times or without adjuvant.Adjuvant is such as not added with, then amount of antigen increases 10-20 times.Compound method：By than
Example puts lanolin and paraffin oil in container, is allowed to mix with ultrasonic wave, autoclaving, puts and saved backup at 5 DEG C.
Before immune animal, first Freund's adjuvant is mixed by a certain percentage with antigen, adjuvant and antigen volume ratio are generally
1：1, it is prepared into " Water-In-Oil " emulsion.Because easily promoting very much it to be emulsified into Water-In-Oil antigen emulsified cpd containing SDS, it is injected into
Emulsified state must be kept when in animal body.Adjuvant can be carried out as follows with antigen emulsification：
(1) polishing：First adjuvant is heated and taken and is put into right amount in sterile glass mortar, is slowly instilled again after cooling
Isometric antigenic solution, ground at same direction in drop, the speed that antigen is added dropwise is slow.After antigen all adds, after
Continuous grinding a period of time, make the sticky water-in-oil emulsion of milky.This law is suitable to prepare substantial amounts of adjuvant antigen, shortcoming
It is that a large amount of emulsions are adhered in mortar wall, antigen losses are larger.
(2) syringe mixing method：The Freund's adjuvant of equivalent and antigenic solution are sucked in two syringes respectively, two injections
It is connected between device with a thin sebific duct, pays attention to emptying air, then alternately promote needle tubing, untill sticky emulsion is formed.This
Method advantage is to be easily able to sterile working, and antigen losses are few, suitable for preparing a small amount of antigen emulsion.But it is difficult to emulsify simultaneously
Entirely, individual antigen, is pushed away motionless at all with plastic injector, and has seepage with glass syringe.
09 month 2012 disclosed in 26 days, Publication No. CN 202447017U Chinese invention disclose it is a kind of new mixed
Close the device of immunologic adjuvant and antigen, it is characterised in that：Described device include mixture portion, scale body portion and suitable for manual force and
The plunger advanced in device, intracavitary sets elastomeric seal in the mixture portion, sets and wears among elastomeric seal
Hole, plunger pass through perforation.This device is a kind of simple syringe.
(3) it is ultrasonic：Laboratory condition is better, such as has Ultrasonic Cell Disruptor, must control supersonic frequency and when
Between, ultrasound easily excites some free radicals, resists original unknown impairment.Emulsification method will be depending on antigen and needs.
Prior art lacks a kind of mixing apparatus of adjuvant easy to use, laborsaving, efficiency high and antigen.
The content of the invention
The technical problem to be solved in the present invention is how to fill up the above-mentioned blank of prior art, there is provided a kind of convenient disassembly,
And the adjuvant and the mixing apparatus of antigen that need not be punched on the ground.
In order to solve the above technical problems, the mixing apparatus of this adjuvant and antigen includes syringe, the syringe includes
Syringe and push rod, syringe front end are provided with nozzle, back end of syringe opening, and the push rod front portion is stretched into syringe inwall, push rod or push away
Liquid-tight envelope between bar front end and syringe inwall, it is characterised in that：It also includes blender, and the blender includes open cup and mixed
Cap mechanisms are closed, wherein mixing cap mechanisms include covering lid, upper stirring cage, lower stirring cage and fixed drive frame, during the cover covers
The heart fixes a vertical pivot, and the upper stirring cage includes upper disk and a plurality of vertical puddler, upper disc centre are provided with axle center hole, and
It is enclosed on by the axle center hole on the vertical pivot, the vertical puddler is uniformly fixed in lower disk surface around axle center hole,
And a upper central gear is additionally provided with upper disk outer rim, the lower surface of upper disk, the lower stirring cage includes lower disc
With multigroup vertical puddler, lower disc center is provided with axle center hole, and is enclosed on by the axle center hole on the vertical pivot, and every group is stirred vertically
Mix that bar is radially even to be fixed in lower disk surface, the vertical puddler of each group is uniformly distributed around axle center hole, lower disc
A lower central gear is additionally provided with upper surface, projection of the upper and lower central gear in same level overlaps, and the two axle center
With vertical pivot axis coinciding, upper disk and vertical puddler form stirring cage, and lower disc and the vertical puddler of each group are formed down and stirred
Cage is mixed, stirring cage of staying is shrouded in upper stirring, and the fixed drive frame comprises at least two meteor gears and motor, two streams
Star gear is clipped between central gear and lower central gear respectively, and is engaged simultaneously with upper central gear and lower central gear,
At least one meteor gear is driven by the motor, and a fluid injection hole is provided with the vertical pivot.
During installation, quantitative adjuvant is first poured into open cup, then cover lid is covered in open cup, upper and lower stirring cage erects
Straight puddler is stretched into adjuvant.Then start motor, fixed drive frame is fixed, meteor gear drive on stir cage,
Lower stirring cage respectively in the direction of the clock, inverse time direction rotates.Then the antigenic solution of predetermined ratio is drawn with syringe, finally
Syringe nozzle is inserted into fluid injection hole, out of, fluid injection hole injection open cup adjuvant.Be so designed that, it is upper stirring cage, under stir
Cage high speed shear, stirring antigenic solution and adjuvant are mixed, the two is formed water-in-oil emulsifier, laborsaving convenience.
As optimization, the level cross-sectionn of the vertical puddler is rectangle, the vertical puddler on upper disk
The tangential direction when long side of rectangle level cross-sectionn rotates is in angle a；Vertical puddler on lower disc it is rectangular
The tangential direction when long side of shape level cross-sectionn rotates is in angle b, and 60 °≤a≤90 °, 45 °≤a≤80 °.So
Design, stirring, emulsifying effectiveness are more preferable.
The mixing apparatus of this adjuvant and antigen creatively using meteor gear in meteor gear mechanism as fixture,
Using central gear as tumbler, motor drives upper and lower central gear difference clockwise, inverse by meteor gear
Hour hands rotate, and vertical puddler high speed shear, stirring antigenic solution and adjuvant on upper and lower stirring cage, quickly form Water-In-Oil
Emulsifying agent, it is design science, simple, convenient laborsaving, as long as staff by simple injection operation, with regard to adjuvant can be completed
With the quick hybrid working of antigen, the process for preparation of various Water-In-Oils, oil-water emulsifiers is widely used in.
Brief description of the drawings
The mixing apparatus of this adjuvant and antigen is described further below in conjunction with the accompanying drawings：
Fig. 1 is cross-sectional view (the section components edge of the mixing apparatus embodiment one of this adjuvant and antigen
Cross the plane cutting of axis)；
Fig. 2 is the present invention looks up structural representation of the upper stirring cage in Fig. 1；
Fig. 3 is the present invention looks up structural representation of the lower stirring cage in Fig. 1；
Elevational schematic view when Fig. 4 is upper stirring cage in Fig. 1, the stirring of lower stirring cage, shearing；
Fig. 5 be the mixing apparatus embodiment two of this adjuvant and antigen upper stirring cage, it is lower stirring cage stirring, cut
Elevational schematic view when cutting.
In figure：1 it is open cup, 2 be cover lid, 3 be vertical pivot, 4 be upper disk, 5 be vertical puddler, 6 be axle center hole, 7 is
Upper central gear, 8 be lower disc, 9 be lower central gear, 10 be meteor gear, 11 be motor, 12 be fluid injection hole, 13
For radial level fixing axle, 14 be Gask-O-Seal, 15 be cover cover handle, 16 be adjuvant, a be vertical on upper disk 4
The angle between tangential direction when the long side of the rectangle level cross-sectionn of puddler 5 rotates；B is on lower disc 8
The angle between tangential direction when the long side of the rectangle level cross-sectionn of vertical puddler 5 rotates.
Embodiment
Embodiment one：As Figure 1-4, the mixing apparatus of this adjuvant and antigen (does not show including syringe in figure
Go out), the syringe includes syringe and push rod, and syringe front end is provided with nozzle, back end of syringe opening, and syringe is stretched into the push rod front portion
In inwall, liquid-tight envelope between push rod or push rod front end and syringe inwall, it is characterised in that：It also includes blender, the mixing
Device includes open cup 1 and mixing cap mechanisms, wherein mixing cap mechanisms include cover lid 2, upper stirring cage, lower stirring cage and fixation
A vertical pivot 3 is fixed at bogie, the center of cover lid 2, and the upper stirring cage includes upper disk 4 and a plurality of vertical puddler 5, on
The center of disk 4 is provided with axle center hole 6, and is enclosed on by the axle center hole 6 on the vertical pivot 3, and the vertical puddler 5 surrounds axle center hole
Uniformly it is fixed on the lower surface of disk 4, and a upper sun is additionally provided with the upper outer rim of disk 4, the lower surface of upper disk 4
Gear 7, the lower stirring cage include lower disc 8 and multigroup vertical puddler 5, and lower disc center is provided with axle center hole 6, and passes through this
Axle center hole is enclosed on the vertical pivot 3, and every group of vertical puddler 5 is radially even to be fixed on the lower surface of disk 4, and each group is vertical
Puddler 5 is uniformly distributed around axle center hole 6, and a lower central gear 9, upper and lower sun tooth are additionally provided with the upper surface of lower disc 8
Projection of the wheel 7,9 in same level overlaps, and the two axle center and vertical pivot axis coinciding, upper disk 4 and the vertical shape of puddler 5
Into upper stirring cage, lower disc 8 and the vertical puddler 5 of each group form lower stirring cage, and stirring cage of staying, the fixation are shrouded in upper stirring
Bogie comprises at least two meteor gears 10 and motor 11, and two meteor gears 10 are clipped in the He of central gear 7 respectively
Between lower central gear 9, and engaged simultaneously with upper central gear 7 and lower central gear 9, at least one meteor gear 10 is by described
Motor 11 is driven, and a fluid injection hole 12 is provided with the vertical pivot 3.
The fixed drive frame also includes a radial level fixing axle 13, the radial level fixing axle 13 directly or
Connect and be fixed on the vertical pivot 3, a meteor gear 10 is enclosed in radial level fixing axle 13, and can be around radial level fixing axle
13 rotate, and are driven meteor gear.One meteor gear 10 is fixed on the output shaft of the motor 11, the motor
11 are directly or indirectly fixed on the vertical pivot 3, and its output shaft axial line overlaps with a RADIAL of vertical pivot 3, by the drive
Dynamic motor 11 drives, and is active meteor gear.
Between upper and lower stirring cage is pointed in the lower end of fluid injection hole 12, the top of fluid injection hole 12 expands, and is embedded with a ring
Shape sealing gasket 14.
The emulsifying agent for preparing is identified to be used, and authentication method is to instill emulsifying agent in cold water, if keep it is complete not
It is scattered, bubbled through the water column into drop-wise, i.e. emulsification is complete, is qualified Water-In-Oil agent.
Embodiment two：As shown in figure 5, the level cross-sectionn of the vertical puddler 5 is rectangle, on upper disk 4
Tangential direction when rotating of the long side of rectangle level cross-sectionn of vertical puddler 5 be in angle a；On lower disc 8
The tangential direction when long side of the rectangle level cross-sectionn of vertical puddler 5 rotates is in angle b, and 60 °≤a≤90 °,
45°≤a≤80°.Remaining structure as described in embodiment 1, is omited.
Claims (3)
1. the mixing apparatus of a kind of adjuvant and antigen, including syringe, the syringe includes syringe and push rod, syringe front end
Provided with nozzle, back end of syringe opening, the push rod front portion is stretched into syringe inwall, between push rod or push rod front end and syringe inwall
Liquid-tight envelope, it is characterised in that：It also includes blender, and the blender includes open cup and mixing cap mechanisms, wherein mixing
Cap mechanisms include cover lid, upper stirring cage, lower stirring cage and fixed drive frame, and a vertical pivot is fixed at the cover lid center, described
Upper stirring cage includes upper disk and a plurality of vertical puddler, and upper disc centre is provided with axle center hole, and is enclosed on institute by the axle center hole
To state on vertical pivot, the vertical puddler is uniformly fixed in lower disk surface around axle center hole, and close upper disk outer rim, on
A upper central gear is additionally provided with the lower surface of disk, the lower stirring cage includes lower disc and multigroup vertical puddler, under
Disc centre is provided with axle center hole, and is enclosed on by the axle center hole on the vertical pivot, every group of vertical radially even fixation of puddler
In upper lower disk surface, the vertical puddler of each group is uniformly distributed around axle center hole, and one is additionally provided with the upper surface of lower disc
Lower central gear, projection of the upper and lower central gear in same level overlap, and the two axle center and vertical pivot axis coinciding, on
Disk and vertical puddler form stirring cage, and lower disc and the vertical puddler of each group form lower stirring cage, and upper stirring is shrouded
Lower stirring cage, the fixed drive frame comprise at least two meteor gears and motor, and two meteor gears are clipped in respectively
Between central gear and lower central gear, and engaged simultaneously with upper central gear and lower central gear, at least one meteor gear
Driven by the motor, a fluid injection hole is provided with the vertical pivot.
2. the mixing apparatus of adjuvant according to claim 1 and antigen, it is characterised in that：The vertical puddler
Level cross-sectionn is rectangle, when the long side of the rectangle level cross-sectionn of the vertical puddler on upper disk rotates
Tangential direction is in angle a；It is tangential when the long side of the rectangle level cross-sectionn of vertical puddler on lower disc rotates
Direction is in angle b, and 60 °≤a≤90 °, 45 °≤a≤80 °.
3. the mixing apparatus of adjuvant according to claim 1 or 2 and antigen, it is characterised in that：The fluid injection hole
Between upper and lower stirring cage is pointed in lower end, expand at the top of the fluid injection hole, and be embedded with a Gask-O-Seal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710765237.2A CN107551866B (en) | 2017-08-30 | 2017-08-30 | Quick mixing device for adjuvant and antigen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710765237.2A CN107551866B (en) | 2017-08-30 | 2017-08-30 | Quick mixing device for adjuvant and antigen |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107551866A true CN107551866A (en) | 2018-01-09 |
| CN107551866B CN107551866B (en) | 2023-08-04 |
Family
ID=60978474
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710765237.2A Active CN107551866B (en) | 2017-08-30 | 2017-08-30 | Quick mixing device for adjuvant and antigen |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107551866B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116099403A (en) * | 2023-04-10 | 2023-05-12 | 赛尔托马斯生物科技(成都)有限公司 | Mixer for antigen and adjuvant and mixing device |
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| CN106823898A (en) * | 2017-02-08 | 2017-06-13 | 贵州大学 | A kind of plain type clay fertilizer mixing arrangement |
| CN206304607U (en) * | 2016-11-30 | 2017-07-07 | 徐州新帝新材料有限公司 | Releasing agent agitating device |
| CN207271124U (en) * | 2017-08-30 | 2018-04-27 | 山东省淡水渔业研究院（山东省淡水渔业监测中心） | The mixing apparatus of adjuvant and antigen |
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2017
- 2017-08-30 CN CN201710765237.2A patent/CN107551866B/en active Active
Patent Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1057405A (en) * | 1990-06-15 | 1992-01-01 | 住友重机械工业株式会社 | Mixer |
| JP2007145706A (en) * | 2005-11-07 | 2007-06-14 | Nippon Steel Corp | Method for kneading monolithic refractory |
| JP2008290035A (en) * | 2007-05-28 | 2008-12-04 | Hitachi Plant Technologies Ltd | Stirring device of deodorizing carrier in bio-deodorization apparatus |
| CN101347752A (en) * | 2008-07-28 | 2009-01-21 | 王洪福 | Stirring-peeling machine and stirring dampener |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116099403A (en) * | 2023-04-10 | 2023-05-12 | 赛尔托马斯生物科技(成都)有限公司 | Mixer for antigen and adjuvant and mixing device |
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| Publication number | Publication date |
|---|---|
| CN107551866B (en) | 2023-08-04 |
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