CN201295600Y - Blending device - Google Patents
Blending device Download PDFInfo
- Publication number
- CN201295600Y CN201295600Y CNU2008203025833U CN200820302583U CN201295600Y CN 201295600 Y CN201295600 Y CN 201295600Y CN U2008203025833 U CNU2008203025833 U CN U2008203025833U CN 200820302583 U CN200820302583 U CN 200820302583U CN 201295600 Y CN201295600 Y CN 201295600Y
- Authority
- CN
- China
- Prior art keywords
- main shaft
- evenly mixing
- antigen
- agitating device
- mixing device
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Mixers Of The Rotary Stirring Type (AREA)
Abstract
The utility model discloses a blending device, which comprises a motor, a main shaft which rotates driven by the motor and a stirring apparatus; the stirring apparatus is connected with the main shaft. The utility model is adapted to blend trace antigens, has the advantages of short time and good effect, and can guarantee the activity and integrity of the antigen proteins.
Description
Technical field
The utility model relates to the mixing that needs form the phase-splitting liquid of stabilising emulsifier, more particularly, relate in the biological field in the Antibody Preparation with antigen with fully or the evenly mixing device of the mixing of Freund.
Background technology
In antibody preparation process; antigen is vital with the degree of mixedness of complete or Freund; its principle is that the abundant mixing of adjuvant and antigen forms a kind of water in oil emulsifying agent; after injecting body; can protect antigen; avoid the antigen tachymetabolism, increase antigen release time, strengthen immune response intensity.
The method of the adjuvant of existing use and antigen emulsification mainly contains following several:
First kind of mixing method is polishing, mainly is suitable for preparing a large amount of adjuvant antigen and mixes.Promptly get the complete or Freund of certain volume and put into aseptic glass mortar; Slowly splash into antigen, whenever add one and should be ground to the droplet disappearance.The speed that drips antigen wants slow, treat that antigen all adds after, should become the water-in-oil emulsion of milky thickness.
The shortcoming of its existence is: adhere to a large amount of emulsions on the mortar wall, antigen losses is big, should not adopt micro-antigen.
Second kind of mixing method is the syringe mixing method, and the adjuvant and the antigen that are about to equivalent suck respectively in two syringes, are connected to then on the three-way pipe, alternately promote the needle tubing mixing, and back and forth operation is till the emulsion that forms thickness.
The shortcoming of its existence is: be difficult for emulsification, the time is long, for unsettled antigen, owing to be exposed to room temperature environment for a long time, will reduce its antigen active, influences immune effect.
The third mixing method is the quick emulsification method, but promptly utilizes ultrasonic disintegrator quick emulsification antigen and adjuvant mixture.Antigen and adjuvant are added in the centrifuge tube in the desired amount, place on the pure device of ultrasonic wave powder, 0.5CM under the pure immersed in liquid level of powder is from the bottom of the bottle about 0.5cm, in order to avoid smash centrifuge tube.Each emulsification 10～15s inserts about refrigerator 1min then.Emulsification can be finished emulsification 3～4 times repeatedly.
The shortcoming of its existence is: destroy the structure of protein in the antigen easily, reduce immune effect.
The utility model content
The utility model provides a kind of evenly mixing device, and this evenly mixing device has and shortens mixing time, stirs fast, operates easily and be applicable to and micro-antigen carried out advantages such as mixing, the activity that guarantees antigen and integrality.
For solving the problems of the technologies described above, the utility model by the following technical solutions: a kind of evenly mixing device, comprise motor, a main shaft and the agitating device that energy rotates under described motor-driven, described agitating device is connected with described main shaft.
The utility model has following technique effect with respect to prior art:
A, shortened mixing time.
Average approximately 30-60 minutes of syringe mode of averaging time spent, got final product at 3-10 minute and utilize this evenly mixing device to carry out the mixing time spent.
B, reduced the loss of antigen emulsifying agent.
Existing polishing only is applicable to a large amount of antigen emulsifying agents of preparation, inapplicable to micro-antigen the method, the utility model is because mixing and injection all are to carry out in same container, guaranteed that the antigen emulsifying agent is from start to finish all in a container, reduced the loss when the antigen emulsifying agent is shifted in another container by a container, so the utility model is applicable to the mixing of micro-antigen.
C, saving manpower.
Syringe mode of averaging and polishing all need manual operations that antigen adjuvant is mixed, and this evenly mixing device is used the motor stirring instead, and method is mixed antigen and adjuvant no longer by hand.
Activity, the integrality of d, assurance antigen protein.
Ultrasonic emulsification is having tangible advantage aspect the loss of time and minimizing antigen emulsifying agent, this method is simple and quick, but there is a drawback in this method, exactly when utilizing ultrasonication, resist and formerly itself can cause some infringements, as: protein active, protein structure etc. destroyed.The utility model can not cause the destruction to protein structure owing to be to carry out mixing by stirring, has guaranteed the activity of antigen protein.
Further, described agitating device can be a connecting rod, and the one end has paddle, and the other end is connected with described main shaft.
Agitating device is arranged to have the connecting rod of paddle, and agitating device is arranged to along axial the comparing of main shaft, embodiments of the present invention are compared with the yi word pattern straight-bar, have bigger stirring area, have the enhancing mixing effect, shorten the advantage of mixing time.
Further, described agitating device is a connecting rod, and the one end has yi word pattern cross bar or cross type cross bar or rice font cross bar.Described cross bar also can be for being other shape beyond the above-mentioned shape, as long as its shape that can realize the effect that stirs all can.
Because the surface area of cross bar is littler, adopt the stirring-head of cross bar shape, can better reduce the loss of antigen and adjuvant.
Further, described agitating device can be a helical form connecting rod, and this helical form connecting rod is connected with main shaft by inserting mode.
The form that agitating device is arranged to insert connection can make things convenient for the dismounting of puddler.
Further, when described agitating device is yi word pattern straight-bar or connecting rod that has paddle or helical form connecting rod or when having the connecting rod of other stirring-head, described agitating device and main shaft axially between have certain included angle.
Agitating device is arranged to have certain angle with main shaft, can realizes antigen and adjuvant are stirred more fully, also have the advantage that can reduce antigen and adjuvant loss.
Further, described mobile device is made up of a guide rail and a lowering or hoisting gear, this lowering or hoisting gear is arranged at an end of described guide rail, and described motor is arranged on the described guide rail, makes described motor do round moving on described guide rail by described lowering or hoisting gear.
Further, described mobile device is a telescopic mounting that is connected with main shaft, and described main shaft is connected with agitating device by this retractor device.
Mobile device can be shifted the emulsion that hangs on the chamber wall bottom of container onto from container top, to reduce the loss of antigen emulsion.
Also can constitute more improvement projects mutually between above-mentioned several improved procedure.
Description of drawings
Below in conjunction with the drawings and specific embodiments the utility model is further described:
Fig. 1 is the utility model overall schematic.
Fig. 2 is an embodiment of agitating device among Fig. 1.
Fig. 3 is another embodiment of agitating device among Fig. 1.
Fig. 4 is another embodiment of agitating device among Fig. 1.
Among the figure: 1, motor 2, guide rail 3, lowering or hoisting gear 4, container 5, main shaft 6, straight-bar 7, solution 8, connecting rod 9, paddle 10, helical form connecting rod
The specific embodiment
As can be seen from Figure 1, evenly mixing device of the present utility model comprises main shaft 5 and agitating device of motor 1, a rotation under motor 1 drives, and described agitating device is connected with main shaft 5.Described agitating device and main shaft 5 can one or are divided body formed preparing.Particularly, described agitating device is a yi word pattern straight-bar 6, this straight-bar 6 and main shaft 5 axially between have certain included angle.Other, described evenly mixing device also can comprise makes described agitating device axially come and go the mobile devices that move along main shaft 5.The agitating device of above-mentioned evenly mixing device is placed one to be held in the container 4 of pending solution 7.Mobile device is made up of a guide rail 2 and a lowering or hoisting gear 3, and this lowering or hoisting gear 3 is arranged at an end of guide rail 2, and motor 1 is arranged on the guide rail 2, makes motor 1 do round moving on guide rail 2 by lowering or hoisting gear 3.Be appreciated that, above-mentioned mobile device can also be a retractor device (not shown) that is connected with main shaft 5, and described main shaft 5 is connected with agitating device by this retractor device, so, can make main shaft 5 do round moving by the telescopic moving of this retractor device.
Agitating device among Fig. 3 is that a connecting rod 8, one ends are connected with paddle 9, and the other end connects with main shaft 5.Paddle 9 has bigger stirring area, has the effect that strengthens mixing effect, and the advantage of less mixing time.In order to reduce by the loss of mixing antigen, the selection of the preparation material of paddle 9 is preferably selected for use with the antigen adhesion or adhesive is bad and the antigen emulsifying agent is not had the material of influence.Equally, for few antigen losses, paddle 9 is gone back yi word pattern straight-bar, and this yi word pattern straight-bar has the characteristics little with the antigen contact area.Above-mentioned agitating device also can be a connecting rod, and the one end has yi word pattern cross bar or cross type cross bar or rice font cross bar.Described cross bar also can be other shape beyond the aforementioned shapes, as long as its shape that can realize the effect that stirs all can.Because the surface area of cross bar is littler, adopt the stirring-head of cross bar shape, can better reduce the loss of antigen and adjuvant.
To be a helical form connecting rod 10, one ends connect with main shaft 5 by inserting mode agitating device among Fig. 4, highly preferred agitating device as shown in Figure 2, promptly agitating device be yi word pattern and main shaft 5 axially have a certain included angle.Be appreciated that, by agitating device being arranged to have certain angle, being had forms such as the connecting rod of paddle and helical form connecting rod with main shaft 5, than agitating device is arranged to along the axial form of main shaft 5, the novel agitating device of this enforcement has better mixing effect, promptly antigen and adjuvant is stirred more fully.
Agitating device in the present embodiment is not limited to Fig. 2 to shape and structure shown in Figure 4; In the reality,, the syringe that pulls out the promotion core can be used as the container 4 among Fig. 1, syringe be installed injection needle a, then injection needle a is fixed on the rubber stopper in order to prevent that syringe from leaking for the ease of operation; In addition, the straight-bar 6 among Fig. 1 can replace with injection needle b, and injection needle b is connected an end of main shaft 5, and injection needle b root is curved certain angle, makes injection needle b axially have a certain included angle with main shaft 5.Be appreciated that container 4 of the present invention is not limited to above-mentioned embodiment, only need guarantee that container 4 is that the closed container of one one end opening gets final product.In addition, can turn round normally in order to guarantee agitating device, more excellent embodiment is the plane, openend place that the tie point of injection needle b and main shaft 5 is not less than closed container.The injection needle b of present embodiment can adopt also that anesthesia syringe needle or puncture needle are first-class to be substituted.
Ratio according to 1:1 adds antigen and complete or Freund, the injection needle b of mounted evenly mixing device is inserted in the syringe, after giving motor 1 energising, motor 1 drives injection needle b and rotates, the injection needle b that rotates carries out stirring and evenly mixing with antigen and adjuvant in syringe, mixing time is about 3-10 minute, stir the later stage, mobile device can make that motor moves up and down along guide rail, injection needle b is promoted from top to bottom, shift the emulsion that hangs on the injector wall onto the syringe bottom with syringe needle, to reduce the loss of antigen emulsion.When the emulsion of the overwhelming majority is pulled to the syringe bottom, syringe is kept flat, the antigen emulsion does not descend flow phenomenon qualified for stirring, and is not thorough if the antigen emulsion has dirty phenomenon then to stir, and need stir, till stirring is qualified again.
Compared with prior art, the utlity model has following advantage:
A, shortened mixing time.
Average approximately 30-60 minutes of syringe mode of averaging time spent, got final product at 3-10 minute and utilize this evenly mixing device to carry out the mixing time spent.
B, reduced the loss of antigen emulsifying agent.
Existing polishing only is applicable to a large amount of antigen emulsifying agents of preparation, inapplicable to micro-antigen the method, the utility model is because mixing and injection all are to carry out in same container, guaranteed that the antigen emulsifying agent is from start to finish all in a container, reduced the loss when the antigen emulsifying agent is shifted in another container by a container, so the utility model is applicable to the mixing of micro-antigen.
C, saving manpower
Syringe mode of averaging and polishing all need manual operations that antigen adjuvant is mixed, and this evenly mixing device is used the motor stirring instead, and method is mixed antigen and adjuvant no longer by hand.
Activity, the integrality of d, assurance antigen protein.
Ultrasonic emulsification is having tangible advantage aspect the loss of time and minimizing antigen emulsifying agent, this method is simple and quick, but there is a drawback in this method, exactly when utilizing ultrasonication, resist and formerly itself can cause some infringements, as: protein active, protein structure etc. destroyed.The utility model can not cause the destruction to protein structure owing to be to carry out mixing by stirring, has guaranteed the activity of antigen protein.
Protection domain of the present utility model is not limited to above-mentioned several embodiment, also can make up mutually between the above-mentioned embodiment.In addition, the other technologies scheme of the simple transformation that can expect of those skilled in the art all belongs to protection domain of the present utility model.
Claims (10)
- [claim 1] a kind of evenly mixing device comprises motor, and one by the main shaft that rotates under the described motor-driven, and it is characterized in that: be provided with an agitating device, described agitating device is connected with described main shaft.
- [claim 2] evenly mixing device according to claim 1 is characterized in that: described puddler is the yi word pattern straight-bar.
- [claim 3] evenly mixing device according to claim 1 is characterized in that: described agitating device is a connecting rod, and the one end has paddle, and the other end is connected with described main shaft.
- [claim 4] evenly mixing device according to claim 1 is characterized in that: described agitating device is a connecting rod, and the one end has yi word pattern cross bar or cross type cross bar or rice font cross bar.
- [claim 5] evenly mixing device according to claim 1 is characterized in that: described agitating device is a helical form connecting rod.
- [claim 6] evenly mixing device according to claim 1 is characterized in that: described agitating device and integrated spindle axis or split setting.
- [claim 7] is characterized in that according to each described evenly mixing device in the claim 1 to 6: described agitating device and main shaft axially between have certain included angle.
- [claim 8] is characterized in that according to each described evenly mixing device in the claim 1 to 6: comprise that one makes described agitating device axially to make the round mobile device that moves along described main shaft.
- [claim 9] evenly mixing device according to claim 8, it is characterized in that: described mobile device is made up of a guide rail and a lowering or hoisting gear, this lowering or hoisting gear is arranged at an end of described guide rail, described motor is arranged on the described guide rail, makes described motor do round moving on described guide rail by described lowering or hoisting gear.
- [claim 10] evenly mixing device according to claim 8 is characterized in that: described mobile device is a retractor device that is connected with main shaft, and described main shaft is connected with agitating device by this retractor device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008203025833U CN201295600Y (en) | 2008-10-30 | 2008-10-30 | Blending device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008203025833U CN201295600Y (en) | 2008-10-30 | 2008-10-30 | Blending device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201295600Y true CN201295600Y (en) | 2009-08-26 |
Family
ID=41041559
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2008203025833U Expired - Fee Related CN201295600Y (en) | 2008-10-30 | 2008-10-30 | Blending device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201295600Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101948113A (en) * | 2010-09-19 | 2011-01-19 | 江西盛丰新能源科技有限公司 | Method and equipment for preparing polycrystalline silicon through physical phosphorous removal |
| CN105255716A (en) * | 2015-11-23 | 2016-01-20 | 广西职业技术学院 | Liquid dripping device |
-
2008
- 2008-10-30 CN CNU2008203025833U patent/CN201295600Y/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101948113A (en) * | 2010-09-19 | 2011-01-19 | 江西盛丰新能源科技有限公司 | Method and equipment for preparing polycrystalline silicon through physical phosphorous removal |
| CN105255716A (en) * | 2015-11-23 | 2016-01-20 | 广西职业技术学院 | Liquid dripping device |
| CN105255716B (en) * | 2015-11-23 | 2018-04-06 | 广西职业技术学院 | Fluid drip device |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104984681B (en) | A kind of agitating device | |
| CN201295600Y (en) | Blending device | |
| CN206298435U (en) | Aeration tank | |
| CN109133553A (en) | A kind of continous way sludge conditioning system and its apparatus | |
| CN101435317B (en) | Lost-return leakage cross-linked gel plugging process | |
| CN204933311U (en) | A kind of injection agitating device | |
| CN103657475A (en) | Agitator convenient to clean | |
| CN109012254B (en) | Emulsification treatment equipment for viscosity reduction of viscous crude oil | |
| CN206199029U (en) | A kind of improved pesticide producing dissolving tank | |
| CN207951267U (en) | A kind of hand operated mixing device of health products | |
| CN203525593U (en) | Antigen adjuvant emulsifying device | |
| CN208003805U (en) | A kind of efficient Deacne pack emulsion reaction tank | |
| CN103446911B (en) | Antigen emulsifying device and emulsification method | |
| CN108514839A (en) | A kind of new material production agitating device | |
| CN207271124U (en) | The mixing apparatus of adjuvant and antigen | |
| CN105771713A (en) | Novel method for emulsifying trace antigen | |
| CN207871932U (en) | A kind of six curved leaf blenders of new structural dislocation | |
| CN203508027U (en) | Chemical engineering reaction tank | |
| CN207750100U (en) | A kind of underground coal mine slip casting component | |
| CN208990727U (en) | A kind of acidification liquid dispensing device | |
| CN207722660U (en) | A kind of miscible device for pharmacy test | |
| CN207276243U (en) | A kind of demister and mixer | |
| CN109701818A (en) | A kind of Double-liquid dynamic-static hybrid system | |
| CN207630302U (en) | A kind of toughener adding apparatus | |
| CN203971817U (en) | A kind of Water-In-Oil albumen blender |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090826 Termination date: 20111030 |