CN107550954A - Application and medicine of the crocodile mouth flower in treatment or prevention neurotrosis medicine is prepared - Google Patents
Application and medicine of the crocodile mouth flower in treatment or prevention neurotrosis medicine is prepared Download PDFInfo
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Abstract
The present invention relates to drug field, the application and the medicine that treat or prevent in neurotrosis medicine is being prepared in particular to a kind of crocodile mouth flower.Crocodile mouth flower is preparing the application in treating or preventing neurotrosis medicine, and neurotrosis is as caused by Parkinson's, alzheimer's disease, headstroke, Protein tau disease.A kind of medicine for being used to prepare neuroprotective agent, it includes crocodile mouth flower.Gradually denaturation occurs for crocodile mouth flower Central nervous member, apoptosis has curative effect.The extract or medicine of crocodile mouth flower have protection neure damage, so as to prevent or postpone the effect of nerve cell death；Disease time can be postponed or delay the development of the state of an illness.Medicine prepared by crocodile mouth flower can cell keep fit form and propagation, suppress Apoptosis；The medicine has significant protective effect for nerve cell.
Description
Technical field
The present invention relates to drug field, and treatment or prevention neurotrosis medicine is being prepared in particular to a kind of crocodile mouth flower
Application and medicine in thing.
Background technology
Crocodile mouth flower spends Clinacanthus nutans for dicotyledon Acanthaceae crocodile mouth flower plant crocodile mouth
(Burm.f.) Lindau dry aerial parts, whole year can harvest.Major function be clearing heat and detoxicating, dampness removing, eliminating stasis to subdue swelling,
Promoting blood circulation for regulating menstruation, analgesic；Cure mainly jaundice, hepatitis ascites, irregular menstruation, treating swelling and pain by traumatic injury, fracture, arthralgia pain due to rheumatism etc..
The emphasis that different regions are used to treat disease is different.In Yunnan, crocodile mouth flower is the Dai nationality's ethnic drug, and medical its of the Dai Nationality continues
Muscle synthetism, treatment traumatic injury and strong Psoralen；In Guangdong and Guangxi Provinces and Hainan, crocodile mouth flower is used as wild vegetable, and medicine food is usually used in using
Treat hepatitis and hepatitis ascites, arthralgia pain due to rheumatism, irregular menstruation, treating swelling and pain by traumatic injury, fracture and leptospirosis.Crocodile mouth flower is other
Country is used to treat kinds of Diseases and also differ greatly, wherein various cancers are treated with it by Malaysia, including lymph cancer, liver cancer,
Lung cancer, kidney, breast cancer, nasopharyngeal carcinoma, uterine cancer, oophoroma, leukemia, the cancer of the brain, prostate cancer etc.；Indonesia is each with its treatment
Kind ephritis, nephrarctia, kidney failure and kidney stone；And bleb, eczema, fash, worm and snake bite are then treated with it by Thailand, and by
It is classified as Thailand's Primary health care medicinal plant.
The content of the invention
It is an object of the invention to provide a kind of crocodile mouth flower prepare treat or prevent neurotrosis medicine in application and
Medicine, it aims to provide a kind of medicine for being used to prepare treatment nervous centralis degenerative disease medicament.
The present invention provides a kind of technical scheme：
Crocodile mouth flower is preparing the application in treating or preventing neurotrosis medicine, and neurotrosis is by Parkinson's, A Erzi
The silent disease in sea, headstroke, caused by Protein tau disease.
Further, application of the extract of above-mentioned crocodile mouth flower as neuroprotective agent.The extract of crocodile mouth flower mainly leads to
Following steps are crossed to be made：Crocodile mouth is spent and extracted using solvent, isolated extract solution after extraction；Wherein, crocodile mouth flower with it is molten
The weight ratio of agent is 1:5~20.
Further, above-mentioned solvent is selected from water, methanol, ethanol, isopropanol, n-butyl alcohol, ethylene glycol, 1,2-PD, 1,
One or more in ammediol, 1,3-BDO, acetone, ethyl acetate.
The present invention also provides a kind of technical scheme：
A kind of medicine for being used to prepare neuroprotective agent, it includes crocodile mouth flower.
Further, said medicine is mainly made by following steps：Crocodile mouth is spent and extracted using solvent, after extraction
Isolated extract solution；Wherein, the weight ratio of crocodile mouth flower and solvent is 1:5~20.
Further, above-mentioned solvent is selected from water, methanol, ethanol, isopropanol, n-butyl alcohol, ethylene glycol, 1,2-PD, 1,
One or more in ammediol, 1,3-BDO, acetone, ethyl acetate.
Further, above-mentioned solvent is that mass fraction is 30-95% ethanol waters.
Further, it is above-mentioned by crocodile mouth flower at 30-90 DEG C ultrasonic extraction 15-60min.
Further, also include after above-mentioned isolated extract solution to extract solution concentration, dry.
Further, it is above-mentioned concentration is evaporated to extract solution, be concentrated under reduced pressure or film concentration.To the extract solution after concentration
Carry out constant pressure and dry, be dried under reduced pressure, vacuum freeze drying or spray drying.
Application and medicine of a kind of crocodile mouth flower provided in an embodiment of the present invention in treatment or prevention neurotrosis medicine is prepared
The beneficial effect of thing is：
Gradually denaturation occurs for crocodile mouth flower Central nervous member, apoptosis has curative effect.The extract or medicine tool of crocodile mouth flower
There is protection neure damage, so as to prevent or postpone the effect of nerve cell death；Disease time can be postponed or delay the state of an illness
Development.Medicine prepared by crocodile mouth flower can cell keep fit form and propagation, suppress Apoptosis；The medicine is thin for nerve
Born of the same parents have significant protective effect.
Brief description of the drawings
In order to illustrate the technical solution of the embodiments of the present invention more clearly, below by embodiment it is required use it is attached
Figure is briefly described, it will be appreciated that the following drawings illustrate only certain embodiments of the present invention, therefore be not construed as pair
The restriction of scope, for those of ordinary skill in the art, on the premise of not paying creative work, can also be according to this
A little accompanying drawings obtain other related accompanying drawings.
Fig. 1 shows that the medicine that the embodiment 1 of mtt assay detection provides induces L-glutamate the protection of HT-22 cells
Effect；
Fig. 2 shows that the medicine that the embodiment 1 of micro- Microscopic observation provides induces HT-22 cells to L-glutamate
Protective effect.
Embodiment
Nervous centralis degenerative disease is one group and denaturation, the god caused by apoptosis is gradually occurred with central nervous system neurons
Through degenerative disease or nervous disorders.Its cause of disease fails to understand that pathogenesis and pathological manifestations are complicated, and clinical manifestation is different mostly.
Nervous centralis degenerative disease is comprehensive including Parkinson's merging dementia, the Alzheimer in Pacific Ocean Guam, Parkinson
Simulator sickness etc..For at present, due to lacking special biochemistry, iconography and pathophysiological features, clinical doctor is often relied primarily on
Raw experience makes diagnosis.In addition to a few diseases (such as Parkinson's) temporarily may improve clinical symptoms by medicine or operation,
Most of diseases there is no with the treatment method of special efficacy at present.
Neuroprotective Therapy in Treating Acute is one or more mechanism by hindering Ischemic Cascade Reaction to be related to, and is protected in the starting stage
Penumbra zone neure damage caused by ischemic, so as to prevent or postpone nerve cell death.It can realize that blood is fast by medicine
Speed passes back into ischemic tissue, so as to suppress the infringement on biochemistry level caused by ischemic, metaboilic level and cellular level.
Such as the therapeutic strategy of ishemic stroke mainly includes thromboembolism treatment and Neuroprotective Therapy in Treating Acute.Thrombolytic drug Ah Ti is general
Enzyme (rt-PA) is first and uniquely ratifies the thrombolytic drug for treating apoplexy by FDA so far.Although rt-PA is
It is proved effectively treat acute ischemic stroke, but its therapeutic time window is narrow, and the mechanism of action is indefinite, there is bleeding risk, because
This only has effective treatment that a few patients can receive rt-PA in time.Benserazide is the choice drug for treating PD, but is suffered from
The severe complications such as on-off phenomenon, the deterioration of agent end occur in person's long-term use.Therefore carry out neuroprotective therapy, may postpone
Disease time or the development for delaying the state of an illness.
, below will be in the embodiment of the present invention to make the purpose, technical scheme and advantage of the embodiment of the present invention clearer
Technical scheme be clearly and completely described.Unreceipted actual conditions person, builds according to normal condition or manufacturer in embodiment
The condition of view is carried out.Agents useful for same or the unreceipted production firm person of instrument, it is the conventional production that can be obtained by commercially available purchase
Product.
Prepared by the application and the medicine that treat or prevent in neurotrosis medicine to the crocodile mouth flower of the embodiment of the present invention below
It is specifically described.
Crocodile mouth flower is preparing the application in treating or preventing neurotrosis medicine, and neurotrosis is by Parkinson's, A Erzi
The silent disease in sea, headstroke, caused by Protein tau disease.
Further, application of the extract of above-mentioned crocodile mouth flower as neuroprotective agent.The extract of crocodile mouth flower mainly leads to
Following steps are crossed to be made：Crocodile mouth is spent and extracted using solvent, isolated extract solution after extraction；Wherein, crocodile mouth flower with it is molten
The weight ratio of agent is 1:5~20.
Further, above-mentioned solvent is selected from water, methanol, ethanol, isopropanol, n-butyl alcohol, ethylene glycol, 1,2-PD, 1,
One or more in ammediol, 1,3-BDO, acetone, ethyl acetate.
Crocodile mouth flower spends Clinacanthusnutans (Burm.f.) for dicotyledon Acanthaceae crocodile mouth flower plant crocodile mouth
Lindau dry aerial parts, whole year can harvest.The major function of crocodile mouth flower is clearing heat and detoxicating, dampness removing, eliminating stasis to subdue swelling, work
Blood menstruation regulating, analgesic；Cure mainly jaundice, hepatitis ascites, irregular menstruation, treating swelling and pain by traumatic injury, fracture, arthralgia pain due to rheumatism etc..
Found by the research and discussion of inventor, crocodile mouth flower is preparing treatment, auxiliary treatment or prevention neurological
Property disease medicine in have it is preferable the effect of.Gradually denaturation occurs for crocodile mouth flower Central nervous member, apoptosis has curative effect.
It should be noted that in an embodiment of the present invention, " neuroprotective agent " refers to help to protect nerve cell to exempt from
By cellular damage and/or resistance neurodegenerative process and the therapeutic agent or active material of neuronal function infringement.
Further, crocodile mouth flower is applied to prepare in the medicine for treating or preventing neurodegenerative disease.Neurodegenerative disease
Including Parkinson's, alzheimer disease, Huntington disease, Protein tau sick (e.g., frontotemporal dementia, cortico-basal degeneration, pik
Family name disease, stein-leventhal syndrome), Wilson's disease, prion disease and other dementia include vascular dementia, amyotrophia funiculus lateralis
Harden (ALS), Acute Stroke (neurotrosis caused by headstroke) and other traumatic injuries；Cerebrovas-cularaccident (e.g., age phase
Closing property macular degeneration)；Brain and trauma of spinal cord, peripheral nerve disease, retinopathy and glaucoma.
The present invention also provides a kind of medicine for being used to prepare neuroprotective agent, and it includes crocodile mouth flower.
Further, the medicine of the present embodiment is mainly made by following steps：Crocodile mouth is spent and extracted using solvent,
Isolated extract solution after extraction；Wherein, the weight ratio of crocodile mouth flower and solvent is 1:5~20.
Further, above-mentioned solvent is selected from water, methanol, ethanol, isopropanol, n-butyl alcohol, ethylene glycol, 1,2-PD, 1,
One or more in ammediol, 1,3-BDO, acetone, ethyl acetate.
Specifically, above-mentioned solvent is mass fraction 30-95% ethanol waters.In the present embodiment, it is mass fraction
95% ethanol water.
Specifically, using ultrasonic extraction, specifically by crocodile mouth flower at 30-90 DEG C ultrasonic extraction 15-60min.
Further, also include after above-mentioned isolated extract solution to extract solution concentration, dry.
Further, it is above-mentioned concentration is evaporated to extract solution, be concentrated under reduced pressure or film concentration.Specifically, using decompression
Concentration.In preferred embodiments of the present invention, it is concentrated by evaporation using Rotary Evaporators.
Constant pressure and dry is carried out to the extract solution after concentration, is dried under reduced pressure, vacuum freeze drying or spray drying.Specifically
Ground, use be dried under reduced pressure in the present invention.
Gradually denaturation occurs for crocodile mouth flower Central nervous member, apoptosis has curative effect.The extract or medicine tool of crocodile mouth flower
There is protection neure damage, so as to prevent or postpone the effect of nerve cell death；Disease time can be postponed or delay the state of an illness
Development.Medicine prepared by crocodile mouth flower can cell keep fit form and propagation, suppress Apoptosis；The medicine is thin for nerve
Born of the same parents have significant protective effect.
In addition, in other embodiments, the medicine also includes pharmaceutically acceptable carrier or auxiliary material.
Such as：Diluent and absorbent, such as starch, dextrin, calcium sulfate, lactose, mannitol, sucrose, sodium chloride, grape
Sugar, urea, calcium carbonate, white bole, microcrystalline cellulose, alumina silicate etc.；Wetting agent and adhesive, as water, glycerine, polyethylene glycol,
Ethanol, propyl alcohol, starch slurry, dextrin, syrup, honey, glucose solution, mucialga of arabic gummy, gelatine size, sodium carboxymethylcellulose,
Lac, methylcellulose, potassium phosphate, polyvinylpyrrolidone etc.；Disintegrant, for example, dry starch, alginate, agar powder,
Laminaran, sodium acid carbonate and citric acid, calcium carbonate, polyoxyethylene, sorbitan fatty acid ester, dodecyl sodium sulfate, first
Base cellulose, ethyl cellulose etc.；Disintegration inhibitor, such as sucrose, glyceryl tristearate, cocoa butter, hydrogenated oil and fat etc.；Absorb
Accelerator, such as quaternary ammonium salt, lauryl sodium sulfate etc.；Lubricant, such as talcum powder, silica, cornstarch, stearic acid
Salt, boric acid, atoleine, polyethylene glycol etc..Tablet can also be further made to coating tablet, such as sugar coated tablet, film bag
Garment piece, enteric coated tablets, or double-layer tablets and multilayer tablet.
In addition, in order to which administration unit is made into pill, various carriers well known in the art can be widely used.Such as：Grape
Sugar, lactose, starch, cocoa butter, hydrogenated vegetable oil, polyvinylpyrrolidone, Gelucire, kaolin, talcum powder etc.；Adhesive
Such as Arabic gum, bassora gum, gelatin, ethanol, honey, liquid sugar, rice paste or batter；Disintegrant, such as agar powder, dry starch,
Alginate, dodecyl sodium sulfate, methylcellulose, ethyl cellulose etc..In order to which administration unit is made into suppository, Ke Yiguang
It is general to use various carriers well known in the art.Example on carrier is, such as polyethylene glycol, lecithin, cocoa butter, advanced
Alcohol, the ester of higher alcohol, gelatin, semi-synthetic glyceride etc..
In addition, if desired, can also be added into pharmaceutical preparation colouring agent, preservative, spices, flavouring, sweetener or
Other materials.
Further, the medicine of the embodiment of the present invention can be administered in a unit, and method of administration can be enteron aisle or non-
Enteron aisle, such as oral, muscle, subcutaneous, nasal cavity, oral mucosa, skin, peritonaeum or rectum.Form of administration for example tablet, capsule,
Dripping pill, aerosol, pill, pulvis, solution, supensoid agent, emulsion, granule, liposome, transdermal agent, buccal tablet, suppository, jelly
Dry powder injection etc..Can be ordinary preparation, sustained release preparation, controlled release preparation and various particulate delivery systems.In order to which unit is administered
Tablet is made in formulation, can widely use various carriers well known in the art.
In addition, the dosage of the medicine of the embodiment of the present invention depends on many factors, such as to be prevented or treat disease
Sex, age, body weight and the individual reaction of the property and the order of severity of disease, patient or animal, particular compound used, administration
Approach and administration number of times etc..Above-mentioned dosage with ingle dose form or can be divided into several, such as two, three or four dosage forms give
Medicine.
From the above, in administration process, the actual dose level of each active component in pharmaceutical composition can be changed, so as to
The reactive compound amount of gained effectively can obtain required therapeutic response for specific patient, composition and administering mode.Dosage
Level must be according to the activity of particular compound, method of administration, the order of severity for treating the patient's condition and the patient's condition of patient to be treated
Selected with medical history.But the way of this area is, the dosage of compound is wanted from less than required therapeutic effect is obtained
The level asked starts, gradual incremental dose, until obtaining required effect.
The feature and performance of the present invention are described in further detail with reference to embodiments.
Embodiment 1
The present embodiment provides a kind of medicine, and the medicine is mainly prepared by following steps：
Crocodile mouth flower 30.11g is weighed, is placed in triangular flask, the ethanol waters of 300ml 95% is added into triangular flask, in 60
DEG C ultrasonic extraction 30min, filtering, is placed in evaporating dish after being condensed into medicinal extract shape with Rotary Evaporators and is evaporated, in constant temperature oven
(50 DEG C) dry to constant weight.
Embodiment 2
The present embodiment provides a kind of medicine, and the medicine is mainly prepared by following steps：
Crocodile mouth flower 10g is weighed, is placed in triangular flask, the ethanol waters of 50ml 30% is added into triangular flask, it is super in 30 DEG C
Sound extracts 15min, filtering, is concentrated under reduced pressure into after medicinal extract shape to be placed in evaporating dish and is evaporated, and perseverance is dried in (50 DEG C) in constant temperature oven
Weight.
Embodiment 3
The present embodiment provides a kind of medicine, and the medicine is mainly prepared by following steps：
Crocodile mouth flower 10g is weighed, is placed in triangular flask, the addition 200ml 60%1 into triangular flask, 2- aqueous solution of propylene glycol,
In 90 DEG C of ultrasonic extraction 60min, filtering, vacuum freeze drying is concentrated under reduced pressure into after medicinal extract shape to constant weight.
Embodiment 4
The present embodiment provides a kind of medicine, and the medicine is mainly prepared by following steps：
Crocodile mouth flower 10g is weighed, is placed in triangular flask, 100ml ethyl acetate is added into triangular flask, in 60 DEG C of ultrasonic extractions
45min, filtering, it is dried under reduced pressure after film concentration to constant weight.
Embodiment 5
The present embodiment provides a kind of medicine, and the medicine is mainly prepared by following steps：
Crocodile mouth flower 10g is weighed, is placed in triangular flask, 150ml water is added into triangular flask, in 45 DEG C of ultrasonic extraction 60min,
Filtering, is placed in evaporating dish after being condensed into medicinal extract shape with Rotary Evaporators and is evaporated, dried to constant weight in (50 DEG C) in constant temperature oven.
Test example
The medicine that this test example obtains to embodiment 1 carries out neuroprotection test.
The culture of 1.HT-22 nerve cells
By (three batches) HT-22 cells, with pancreatin (0.25%, 1mL), therefrom ware digests (in 1min), dispels into slender
Born of the same parents' suspension, add a small amount of hyclone FBS (100 μ L).1000rpm centrifuges 5min, removes supernatant, cell precipitation is dispersed in into 3mL
In complete medium, take 1mL cell suspensions to be added in 24mL complete mediums, overturn 10 mixings back and forth, take 0.1mL to be used for
Count.It is diluted to appropriate cell concentration (8 × 104Cell/mL after), 48 orifice plates first add 50 μ L culture mediums, make culture medium thorough
It is distributed in orifice plate rims.Be planted in 48 orifice plates (three batches of cells, three blocks of plates, 100 μ L, 8 × 104cell/mL).Rotated in kind
Centrifuge tube, one half bore of kind firmly blow and beat cell, are allowed to uniform, while allow cell suspension to be paved with orifice plate bottom, after all finishing
Uniformly whether micro- Microscopic observation cell scattered, and cross, which shakes up, makes cell be uniformly dispersed in orifice plate bottom.Place 24 hours (cells
Degrees of fusion is about 30~40%) it is used to test.
2.MTT methods are tested
It is scattered in the μ g of medicine 200 are obtained in embodiment 1 in 2mL complete mediums, changes liquid for 3 × 48 orifice plates, be MTT
Experiment.Kind of 48 orifice plates of good cell are taken, are divided into Control (control group), Glutamate (glutamic acid) group, NAC (N-acetyl
Cysteine) (N-acetylcystein), Trolox (watermiscible vitamin E) groups and the sample sets (medicine that embodiment 1 provides
Group).Every group of three multiple holes, culture medium change liquid, per the μ L of hole 200, before changing liquid every time, blow and beat culture medium, make dispersion of medicine.Change
Addition glutamic acid makes final concentration reach 3mM (6 μ L) after complete 37 DEG C of liquid is incubated 1h.37 DEG C of culture 24h are placed on after shaking up.Observation is thin
Born of the same parents' form, and cell survival rate (mtt assay) is detected, then with 20 μ L MTT (5mg/mL) lucifuge, 37 DEG C of incubation 2h, gently suck
Liquid, 200 μ L DMSO, shaking table 5min is added to shake up per hole.Then it is (following to calculate each light absorption value under 490nm with multi-function microplate reader
Data are to be measured under 490nm in figure, damage group cell survival rate be about Control groups 20%).Three hole OD values are taken, ask flat
Average, subtract blank DMSO OD values, then divided by control group standardizing average values.The MTT of three batches of cells OD values are put down
Average does statistical discrepancy with SigmaPlot12.5 softwares.
Statistical result is as shown in figure 1, specifically, Fig. 1 shows the crocodile mouth flower extract of mtt assay detection to L-
Glutamate induces the protective effect of HT-22 cells."+", which represents corresponding test group, in figure corresponding component, and "-" represents
Corresponding test group does not have corresponding component.Wherein, * * P<0.01, * * * P<0.001 compared with glutamic acid group (single factor test variance
Analysis, Dunnett methods).Trolox (10 μM) uses as positive control.
Shown by Fig. 1 result, the medicine that embodiment 1 provides has significant protective effect for HT-22 nerve cells.
3. the protective effect of the crocodile mouth flower extract of micro- Microscopic observation
The μ g of medicine 20 that embodiment 1 provides are scattered in 2mL complete mediums, and liquid is changed for 3 × 48 orifice plates.Take kind good thin
48 orifice plates of born of the same parents, are Control groups respectively, Glutamate groups, Glu (Glutamate)+NAC (N-acetyl cysteine)
Group, Glu+Trolox and Glu+ sample sets (medicine group that embodiment 1 provides), every group of three multiple holes, culture medium changes liquid, per hole
200 μ L, before changing liquid every time, culture medium is blown and beaten, makes dispersion of medicine.Changing addition glutamic acid after complete 37 DEG C of liquid is incubated 1h makes end dense
Degree reaches 3mM (6 μ L).37 DEG C of culture 24h are placed on after shaking up.Cellular morphology is finally observed under the microscope, and is taken pictures, as a result
As shown in Figure 2.Fig. 2 shows that the crocodile mouth flower extract of micro- Microscopic observation induces L-glutamate the protection of HT-22 cells
Effect.As shown in Figure 2, the medicine that embodiment 1 provides can make cell keep fit form and propagation, it is suppressed that L-
Apoptosis caused by glutamate (Pidolidone).
It was found from this experimental example, medicine that embodiment 1 provides can cell keep fit form and propagation, suppress cell and wither
Die；The medicine has significant protective effect for HT-22 nerve cells.
The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the invention, for the skill of this area
For art personnel, the present invention can have various modifications and variations.Within the spirit and principles of the invention, that is made any repaiies
Change, equivalent substitution, improvement etc., should be included in the scope of the protection.
Claims (10)
1. crocodile mouth flower is preparing the application in treating or preventing neurotrosis medicine, it is characterised in that the neurotrosis is by pa
The gloomy disease of gold, alzheimer's disease, headstroke, caused by Protein tau disease.
2. application according to claim 1, it is characterised in that the extract of the crocodile mouth flower is preparing treatment or prevention god
Through the application in damage medicine；
The extract of the crocodile mouth flower is mainly made by following steps：Crocodile mouth is spent and extracted using solvent, is divided after extraction
From obtaining extract solution；Wherein, the weight ratio of the crocodile mouth flower and the solvent is 1:5~20.
3. application according to claim 2, it is characterised in that the solvent is selected from water, methanol, ethanol, isopropanol, 1- fourths
One or more in alcohol, ethylene glycol, 1,2-PD, 1,3-PD, 1,3-BDO, acetone, ethyl acetate.
4. a kind of medicine for being used to prepare neuroprotective agent, it is characterised in that the medicine is spent including crocodile mouth.
5. the medicine according to claim 4 for being used to prepare neuroprotective agent, it is characterised in that the medicine mainly passes through
Following steps are made：The crocodile mouth is spent and extracted using solvent, isolated extract solution after extraction；Wherein, the crocodile mouth
The weight ratio of flower and the solvent is 1:5~20.
6. according to claim 5 be used to prepare the medicine of neuroprotective agent, it is characterised in that the solvent be selected from water,
Methanol, ethanol, isopropanol, n-butyl alcohol, ethylene glycol, 1,2-PD, 1,3-PD, 1,3-BDO, acetone, ethyl acetate
In one or more.
7. the medicine according to claim 5 for being used to prepare neuroprotective agent, it is characterised in that the solvent is quality point
Number is 30-95% ethanol waters.
8. the medicine according to claim 5 for being used to prepare neuroprotective agent, it is characterised in that the crocodile mouth flower exists
Ultrasonic extraction 15-60min at 30-90 DEG C.
9. the medicine according to claim 5 for being used to prepare neuroprotective agent, it is characterised in that the isolated extraction
Also include after liquid to extract solution concentration, dry.
10. the medicine according to claim 9 for being used to prepare neuroprotective agent, it is characterised in that enter to the extract solution
Row be concentrated by evaporation, be concentrated under reduced pressure or film concentration；Constant pressure and dry is carried out to the extract solution after concentration, is dried under reduced pressure, vacuum
Freeze-drying or spray drying.
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Cited By (1)
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| CN107362185A (en) * | 2017-07-27 | 2017-11-21 | 广州中医药大学 | Application and medicine of the little Hua Asystasia gangetica (L) Anders.(A. Coromandelihas Nees)s in the medicine for preparing prevention and/or treatment nerve degenerative diseases |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI779941B (en) * | 2021-11-30 | 2022-10-01 | 衛生福利部國家中醫藥研究所 | Extraction method of clinacanthus nutans, and use of it's extract for treating osteoporosis, neurodegenerative diseases, skeletal muscle atrophy and improving wound healing |
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