CN106728476A - Treat the pharmaceutical composition of diabetic retinopathy - Google Patents
Treat the pharmaceutical composition of diabetic retinopathy Download PDFInfo
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- CN106728476A CN106728476A CN201611133161.3A CN201611133161A CN106728476A CN 106728476 A CN106728476 A CN 106728476A CN 201611133161 A CN201611133161 A CN 201611133161A CN 106728476 A CN106728476 A CN 106728476A
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- ethanol
- pharmaceutical composition
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
- A61K31/365—Lactones
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/87—Vitaceae or Ampelidaceae (Vine or Grape family), e.g. wine grapes, muscadine or peppervine
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Abstract
Pharmaceutical composition of diabetic retinopathy and preparation method thereof is treated the invention discloses a kind of, pharmaceutical composition of the present invention is with erioflorin acetate, three-jaw gold dragon, katydid as bulk drug, proportioning is formed, routinely various formulations can be made by preparation process, treatment diabetic retinopathy is evident in efficacy.
Description
Technical field
The invention belongs to technical field of traditional Chinese medicines, more particularly to a kind of pharmaceutical composition for treating diabetic retinopathy and
Its preparation method.
Background technology
Diabetes (DM) are to increase the metabolic disease group being characterized with blood sugar, and BDR (DR) is that its is tight
One of complication of weight, is one of main retinopathy in blinding illness in eye.Research shows, the degree of DR and the course of disease of DM, blood
Sugared controlled level is proportionate.The eyeground pathological changes of DR be mainly microaneurysm, bleeding, ooze out, oedema, capillary are inaccessible, new
Angiogenic propagation etc. and shows as the DPNs such as defect of visual field at ischemic microangiopathies.In recent years, Chinese medicine DR
With improving microcirculation, protection vascular wall, reduce oedema and ooze out and the features such as toxic and side effect is small, increasingly closed
Note, Chinese herb decoction opinion is controlled, and especially to early stage PVR curative effect preferably, advantage is unique.
Erioflorin acetate（Erioflorin acetate）：CAS 27542-23-0, molecular formula
C21H26O7, molecular weight 390.43.【Pharmacological action】There are human body nasopharyngeal carcinoma (KB) cell and mouse lymphocyte leukaemia
The effect of P388 (PS).【Purposes】It is antitumor.【Ingredient origin】Feverfew handle flower chrysanthemum Podanthus ovatifohus
Lag stems, leaf.
Three-jaw gold dragon：It is Vitaceae Parthenocissus plant parthenocissus himalayana Plancs Parthenocissus himalayana
（Royle）Planch. the complete stool of ［ Ampelopsis himalayana Royle ］.Autumn, winter harvesting root and stem, cut after cleaning
Piece or segment, dry.Summer, autumn picking leaves, dry.【Nature and flavor】It is pungent；Temperature.【Indication】Dispelling wind and eliminating dampness；Dissipate blood stasis and smoothing collaterals.Main wind
Arthritis with fixed pain caused by dampness is bitterly；Traumatic injury；Fracture.【Former phytomorph】Parthenocissus himalayana Plancs fallen leaves woody climber.Multi-branched, branch is sturdy, drabon
Color；Tendril is short and branch, and there is sucker on top.The leaflet alternate of palmate 3；Common petiole 5-12cm long, without hair or hairiness；Blade papery,
Middle leaflet is avette or width drape over one's shoulders needle-like avette, 6-12cm long, 2-7cm wide, and tip is tapering or nearly shape of tail, and base portion wedge shape, side life is small
Leaf is asymmetric, tiltedly avette, compared with the middle of leaflet it is smaller, edge have substantially and the sawtooth with tip, above without hair, below or along vein
There is thin pubescence；Petiolule 5-6mm long.Flower both sexes, cyme be often born in brachyplast top or with leaf opposite；Bennet is compared with petiole
It is short；Flower light green, 5 numbers 4 are counted sometimes；Calyx shallow disc shape, full edge；Petal Long Circle；Stamen and petal opposite；Floral disc is not obvious；
Style short cylindrical shape, tip often expands discoid after petal comes off.Berry is spherical, ripe time-varying dark brown, has white powder, seed 1-2
.Record in dictionary of medicinal plant.
Katydid：This product is Tettigoniidae katydid category animal katydid Gampsaocleis gratiosa Brunner
Wattenwyl. entirety.Summer, autumn catch, and catch rear boiling water and scald dead, dry or dry.【Nature and flavor】Taste is pungent；Micro-sweet；It is mild-natured.【Work(
Can cure mainly】Inducing diuresis for removing edema；Remove obstruction in channels to relieve pain.Main oedema oliguria；Waist and knee swells and ache；Weak foot due to dampness.【Proterties】It is in Long Circle, ash that this product is all
Green or yellowish-brown.Slightly rounded, 1 pair, the compound eye of head, oval, feeler 1 pair, length is whiplike, comes off more；Pronotary is slightly in elongated
Cylinder, middle metathorax is by wing；Pereiopoda 3 pairs, comes off more, and metapedes is larger.Gas raw meat.Record in dictionary of medicinal plant.
1 bulk drug chemical constitution：
Erioflorin acetate（Erioflorin acetate）.
The content of the invention
The purpose of the present invention is to overcome the shortcomings of background technology, there is provided a kind of medicine of effective treatment diabetic retinopathy
Compositions and preparation method thereof.
The present invention adopts the following technical scheme that realization：
The composition and weight portion for being made the bulk drug of the pharmaceutical composition of the treatment diabetic retinopathy be：
Erioflorin acetate 7-10 weight portions three-jaw gold dragon 3084-3100 weight portion katydid 2100-2200 weight
Part.
The pharmaceutical composition for the treatment of diabetic retinopathy is preferably used in, is by the bulk drug group of following weight portion
Into：
The imperial weight portion of 3090 weight portion katydid 2150 of the weight portion three-jaw of erioflorin acetate 8 gold.
A kind of pharmaceutical composition for treating diabetic retinopathy, it is characterised in that pharmaceutical composition can use preparation
Conventional method prepares piece agent or capsule or dripping pill.
A kind of pharmaceutical composition for treating diabetic retinopathy, it is characterised in that pharmaceutical composition and chemical drugs or in
The treatment diabetic retinopathy medicine of medicine composition.
A kind of preparation method of the pharmaceutical composition for treating diabetic retinopathy, it is characterised in that make as follows
It is standby：
The composition and weight portion of bulk drug be：Erioflorin acetate 7-10 weight portions three-jaw gold dragon 3084-3100 weights
Amount part katydid 2100-2200 weight portions；
Preparation method：
（1）Erioflorin acetate, three-jaw gold dragon, katydid are taken by bulk drug proportioning, is mixed, use weight percent concentration
33% ethanol takes as solvent in 35.5 DEG C of temperature extractions, and extraction time is 16 times, and each extraction time is 24 hours, each solvent
Consumption is 46 times of bulk drug gross weight, is filtered, and obtains dregs of a decoction A and extract solution A, and extract solution A reclaims ethanol, is concentrated into relative density
1.06, filtration, liquid is first washed with water by DA-201 large pore resin absorption columns, then molten with the ethanol of weight percent concentration 19%
Liquid wash-out DA-201 large pore resin absorption columns, the collection ethanol eluate of weight percent concentration 19%, recovery ethanol, concentrate drying,
Obtain final product extract A；
（2）Take step（1）Dregs of a decoction A, with the ethanol of weight percent concentration 47% as solvent, heating and refluxing extraction 16 times is carried every time
The time is taken for 0.3 hour, each solvent load is 44 times of dregs of a decoction A weight, and filtration obtains dregs of a decoction B and extract solution B, and extract solution B is returned
Ethanol is received, relative density 1.14 is concentrated into, filtered, liquid is first washed with water, then use by LSD-280 large pore resin absorption columns
The ethanol solution of weight percent concentration 63% elutes LSD-280 large pore resin absorption columns, collects the ethanol of weight percent concentration 63%
Eluent, reclaims ethanol, and concentrate drying obtains final product extract B；
（3）Extract A and extract B are mixed, pharmaceutical composition is obtained final product.
Preferred a kind of preparation method of the pharmaceutical composition for treating diabetic retinopathy, it is characterised in that by as follows
It is prepared by step：
The composition and weight portion of bulk drug be：The imperial 3090 weight portion Guo of the weight portion three-jaw of erioflorin acetate 8 gold
The weight portions of Guo 2150；
Preparation method：
（1）Erioflorin acetate, three-jaw gold dragon, katydid are taken by bulk drug proportioning, is mixed, use weight percent concentration
33% ethanol takes as solvent in 35.5 DEG C of temperature extractions, and extraction time is 16 times, and each extraction time is 24 hours, each solvent
Consumption is 46 times of bulk drug gross weight, is filtered, and obtains dregs of a decoction A and extract solution A, and extract solution A reclaims ethanol, is concentrated into relative density
1.06, filtration, liquid is first washed with water by DA-201 large pore resin absorption columns, then molten with the ethanol of weight percent concentration 19%
Liquid wash-out DA-201 large pore resin absorption columns, the collection ethanol eluate of weight percent concentration 19%, recovery ethanol, concentrate drying,
Obtain final product extract A；
（2）Take step（1）Dregs of a decoction A, with the ethanol of weight percent concentration 47% as solvent, heating and refluxing extraction 16 times is carried every time
The time is taken for 0.3 hour, each solvent load is 44 times of dregs of a decoction A weight, and filtration obtains dregs of a decoction B and extract solution B, and extract solution B is returned
Ethanol is received, relative density 1.14 is concentrated into, filtered, liquid is first washed with water, then use by LSD-280 large pore resin absorption columns
The ethanol solution of weight percent concentration 63% elutes LSD-280 large pore resin absorption columns, collects the ethanol of weight percent concentration 63%
Eluent, reclaims ethanol, and concentrate drying obtains final product extract B；
（3）Extract A and extract B are mixed, pharmaceutical composition is obtained final product.
A kind of preparation method of the pharmaceutical composition for treating diabetic retinopathy, it is characterised in that pharmaceutical composition can
Piece agent or capsule or dripping pill are prepared with using the conventional method of galenic pharmacy.
A kind of preparation method of the pharmaceutical composition for treating diabetic retinopathy, it is characterised in that pharmaceutical composition with
Chemical drugs or Chinese medicine composition treatment diabetic retinopathy medicine.
Medicine composite for curing diabetic retinopathy is evident in efficacy.
Specific embodiment
Embodiment 1：Treat pharmaceutical composition of diabetic retinopathy and preparation method thereof
Treat diabetic retinopathy pharmaceutical composition bulk drug composition and weight portion be：Erioflorin vinegar
Acid esters 8g three-jaws gold dragon 3090g katydids 2150g；
Preparation method：
（1）Erioflorin acetate, three-jaw gold dragon, katydid are taken by bulk drug proportioning, is mixed, use weight percent concentration
33% ethanol takes as solvent in 35.5 DEG C of temperature extractions, and extraction time is 16 times, and each extraction time is 24 hours, each solvent
Consumption is 46 times of bulk drug gross weight, is filtered, and obtains dregs of a decoction A and extract solution A, and extract solution A reclaims ethanol, is concentrated into relative density
1.06, filtration, liquid is first washed with water by DA-201 large pore resin absorption columns, then molten with the ethanol of weight percent concentration 19%
Liquid wash-out DA-201 large pore resin absorption columns, the collection ethanol eluate of weight percent concentration 19%, recovery ethanol, concentrate drying,
Obtain final product extract A；
（2）Take step（1）Dregs of a decoction A, with the ethanol of weight percent concentration 47% as solvent, heating and refluxing extraction 16 times is carried every time
The time is taken for 0.3 hour, each solvent load is 44 times of dregs of a decoction A weight, and filtration obtains dregs of a decoction B and extract solution B, and extract solution B is returned
Ethanol is received, relative density 1.14 is concentrated into, filtered, liquid is first washed with water, then use by LSD-280 large pore resin absorption columns
The ethanol solution of weight percent concentration 63% elutes LSD-280 large pore resin absorption columns, collects the ethanol of weight percent concentration 63%
Eluent, reclaims ethanol, and concentrate drying obtains final product extract B；
（3）Extract A and extract B are mixed, pharmaceutical composition is obtained final product.
Embodiment 2：Treat pharmaceutical composition of diabetic retinopathy and preparation method thereof
Treat diabetic retinopathy pharmaceutical composition bulk drug composition and weight portion be：Erioflorin vinegar
Acid esters 7g three-jaws gold dragon 3100g katydids 2100g；
Preparation method：
（1）Erioflorin acetate, three-jaw gold dragon, katydid are taken by bulk drug proportioning, is mixed, use weight percent concentration
33% ethanol takes as solvent in 35.5 DEG C of temperature extractions, and extraction time is 16 times, and each extraction time is 24 hours, each solvent
Consumption is 46 times of bulk drug gross weight, is filtered, and obtains dregs of a decoction A and extract solution A, and extract solution A reclaims ethanol, is concentrated into relative density
1.06, filtration, liquid is first washed with water by DA-201 large pore resin absorption columns, then molten with the ethanol of weight percent concentration 19%
Liquid wash-out DA-201 large pore resin absorption columns, the collection ethanol eluate of weight percent concentration 19%, recovery ethanol, concentrate drying,
Obtain final product extract A；
（2）Take step（1）Dregs of a decoction A, with the ethanol of weight percent concentration 47% as solvent, heating and refluxing extraction 16 times is carried every time
The time is taken for 0.3 hour, each solvent load is 44 times of dregs of a decoction A weight, and filtration obtains dregs of a decoction B and extract solution B, and extract solution B is returned
Ethanol is received, relative density 1.14 is concentrated into, filtered, liquid is first washed with water, then use by LSD-280 large pore resin absorption columns
The ethanol solution of weight percent concentration 63% elutes LSD-280 large pore resin absorption columns, collects the ethanol of weight percent concentration 63%
Eluent, reclaims ethanol, and concentrate drying obtains final product extract B；
（3）Extract A and extract B are mixed, pharmaceutical composition is obtained final product.
Embodiment 3：Treat pharmaceutical composition of diabetic retinopathy and preparation method thereof
Treat diabetic retinopathy pharmaceutical composition bulk drug composition and weight portion be：Erioflorin vinegar
Acid esters 10g three-jaws gold dragon 3084g katydids 2200g；
Preparation method：
（1）Erioflorin acetate, three-jaw gold dragon, katydid are taken by bulk drug proportioning, is mixed, use weight percent concentration
33% ethanol takes as solvent in 35.5 DEG C of temperature extractions, and extraction time is 16 times, and each extraction time is 24 hours, each solvent
Consumption is 46 times of bulk drug gross weight, is filtered, and obtains dregs of a decoction A and extract solution A, and extract solution A reclaims ethanol, is concentrated into relative density
1.06, filtration, liquid is first washed with water by DA-201 large pore resin absorption columns, then molten with the ethanol of weight percent concentration 19%
Liquid wash-out DA-201 large pore resin absorption columns, the collection ethanol eluate of weight percent concentration 19%, recovery ethanol, concentrate drying,
Obtain final product extract A；
（2）Take step（1）Dregs of a decoction A, with the ethanol of weight percent concentration 47% as solvent, heating and refluxing extraction 16 times is carried every time
The time is taken for 0.3 hour, each solvent load is 44 times of dregs of a decoction A weight, and filtration obtains dregs of a decoction B and extract solution B, and extract solution B is returned
Ethanol is received, relative density 1.14 is concentrated into, filtered, liquid is first washed with water, then use by LSD-280 large pore resin absorption columns
The ethanol solution of weight percent concentration 63% elutes LSD-280 large pore resin absorption columns, collects the ethanol of weight percent concentration 63%
Eluent, reclaims ethanol, and concentrate drying obtains final product extract B；
（3）Extract A and extract B are mixed, pharmaceutical composition is obtained final product.
Embodiment 4：The preparation of tablet
The pharmaceutical composition 209g of Example 1, adds starch 212, mixes, and granulation is dried, plus microcrystalline cellulose 123g, stearic
Sour magnesium 3g, mixes, and is pressed into 1600, obtains final product medicinal composition tablets.
Embodiment 5：The preparation of capsule
The pharmaceutical composition 224g of Example 2, adds starch 190g, mixes, and granulation is dried, whole grain, adds appropriate stearic acid
Magnesium, mixes, and encapsulated 1200, obtains final product medicament composition capsule.
Embodiment 6：The preparation of dripping pill
Weigh (80 DEG C) heating of Macrogol 6000 200g water-baths and boil molten, add the pharmaceutical composition 10g of embodiment 3, fully stir
Mix uniform, with atoleine as cooling agent, put glass tube（4*80cm）In, chilling temperature is 7 DEG C, and drip internal-and external diameter is 7.0/
2.0 (mm/mm), drip is 2.7cm away from liquid level, and drop speed is optimum condition with every point 54 drop, and the cold of dripping pill surface is blotted with cotton
Solidifying agent, obtains final product medicament composition dropping pills.
Experimental example 1：Treat the experimental study of diabetic retinopathy
1 clinical data
1.1 physical data
Case is respectively the hospital of traditional Chinese hospital of Jinan City ophthalmic patient, and 38 patients are on diabetes diagnosis according to the World Health Organization
It is that standard is made a definite diagnosis and meet what ophthalmology branch of Chinese Medical Association in 1984 formulated《Stages of DR standard》, it is
Simple form.Treatment group and each 19 of control group, in treatment group man 9, female 10, average age (50.65 ± 6.45) year, blood sugar
(10.45±2.11)mmol/L.In control group man 10, female 9, average age (51.25 ± 7.12) year, blood sugar (9.88 ±
1.66)mmol/L.Random packet, statistical procedures no significant difference.Inspection method:38 patients are in before treatment and after treatment
The measure for carrying out eyesight, funduscopy and Hemorheology in 1 month, including:WBV, cholesterol and triglycerides.
1.2 treatment methods
Western medicine group:Antidiabetic drug is oral, and consumption is adjusted according to blood sugar level, and Tanakan is oral daily 2 ~ 3；Treatment group:Antidiabetic drug
Orally, combination of oral medication（The pharmaceutical composition of embodiment 1）, each 2g, 3 times a day, is within 1 month 1 course for the treatment of, two groups of medication periods
Between hyperpietic to give depressor oral.
1.3 efficacy determination indexs
1) eyesight:Effective, eyesight is improved more than 3 rows；Take a turn for the better, improve 1 ~ 3 row；It is invalid, do not improve or decline；2) eyeground:It is effective,
Eyeground microaneurysm, bleeding is oozed out area and is disappeared up to more than 1/2；Take a turn for the better, eyeground microaneurysm, bleeding, ooze out area disappear reach
1/4~1/2；It is invalid:Eyeground microaneurysm, bleeding, ooze out and do not disappear or aggravate；3) change of Hemorheology:WBV, courage
Sterol and triglycerides.
1.4 testing results
The results are shown in Table 1-2.
Change of the medicine composite for curing pure diabetes of table 1 to eyesight
| Group | n | It is effective | Take a turn for the better | It is invalid |
| Western medicine group | 19 | 4 | 13 | 2 |
| Treatment group | 19 | 10# | 8 | 1 |
Note:Compare #P with Western medicine group<0.05.
Change of the medicine composite for curing pure diabetes of table 2 to eyeground
| Group | n | It is effective | Take a turn for the better | It is invalid |
| Western medicine group | 19 | 5 | 10 | 4 |
| Treatment group | 19 | 14# | 4 | 1 |
Note:Compare #P with Western medicine group<0.05.
Claims (8)
1. a kind of pharmaceutical composition for treating diabetic retinopathy, it is characterised in that be made the bulk drug of the pharmaceutical composition
Composition and weight portion be：
Erioflorin acetate 7-10 weight portions three-jaw gold dragon 3084-3100 weight portion katydid 2100-2200 weight
Part.
2. a kind of pharmaceutical composition for treating diabetic retinopathy according to claim 1, it is characterised in that be made this
The composition and weight portion of the bulk drug of pharmaceutical composition be：
The imperial weight portion of 3090 weight portion katydid 2150 of the weight portion three-jaw of erioflorin acetate 8 gold.
3. a kind of pharmaceutical composition for treating diabetic retinopathy according to claim 1, it is characterised in that medicine group
Compound can prepare piece agent or capsule or dripping pill using the conventional method of galenic pharmacy.
4. a kind of pharmaceutical composition for treating diabetic retinopathy according to claim 1, it is characterised in that medicine group
The treatment diabetic retinopathy medicine that compound is constituted with chemical drugs or Chinese medicine.
5. a kind of preparation method of the pharmaceutical composition for treating diabetic retinopathy, it is characterised in that make as follows
It is standby：
The composition and weight portion of bulk drug be：Erioflorin acetate 7-10 weight portions three-jaw gold dragon 3084-3100 weights
Amount part katydid 2100-2200 weight portions；
Preparation method：
（1）Erioflorin acetate, three-jaw gold dragon, katydid are taken by bulk drug proportioning, is mixed, use weight percent concentration
33% ethanol takes as solvent in 35.5 DEG C of temperature extractions, and extraction time is 16 times, and each extraction time is 24 hours, each solvent
Consumption is 46 times of bulk drug gross weight, is filtered, and obtains dregs of a decoction A and extract solution A, and extract solution A reclaims ethanol, is concentrated into relative density
1.06, filtration, liquid is first washed with water by DA-201 large pore resin absorption columns, then molten with the ethanol of weight percent concentration 19%
Liquid wash-out DA-201 large pore resin absorption columns, the collection ethanol eluate of weight percent concentration 19%, recovery ethanol, concentrate drying,
Obtain final product extract A；
（2）Take step（1）Dregs of a decoction A, with the ethanol of weight percent concentration 47% as solvent, heating and refluxing extraction 16 times is carried every time
The time is taken for 0.3 hour, each solvent load is 44 times of dregs of a decoction A weight, and filtration obtains dregs of a decoction B and extract solution B, and extract solution B is returned
Ethanol is received, relative density 1.14 is concentrated into, filtered, liquid is first washed with water, then use by LSD-280 large pore resin absorption columns
The ethanol solution of weight percent concentration 63% elutes LSD-280 large pore resin absorption columns, collects the ethanol of weight percent concentration 63%
Eluent, reclaims ethanol, and concentrate drying obtains final product extract B；
（3）Extract A and extract B are mixed, pharmaceutical composition is obtained final product.
6. a kind of preparation method of the pharmaceutical composition for treating diabetic retinopathy, its feature according to claim 5
It is to prepare as follows：
The composition and weight portion of bulk drug be：The imperial 3090 weight portion Guo of the weight portion three-jaw of erioflorin acetate 8 gold
The weight portions of Guo 2150；
Preparation method：
（1）Erioflorin acetate, three-jaw gold dragon, katydid are taken by bulk drug proportioning, is mixed, use weight percent concentration
33% ethanol takes as solvent in 35.5 DEG C of temperature extractions, and extraction time is 16 times, and each extraction time is 24 hours, each solvent
Consumption is 46 times of bulk drug gross weight, is filtered, and obtains dregs of a decoction A and extract solution A, and extract solution A reclaims ethanol, is concentrated into relative density
1.06, filtration, liquid is first washed with water by DA-201 large pore resin absorption columns, then molten with the ethanol of weight percent concentration 19%
Liquid wash-out DA-201 large pore resin absorption columns, the collection ethanol eluate of weight percent concentration 19%, recovery ethanol, concentrate drying,
Obtain final product extract A；
（2）Take step（1）Dregs of a decoction A, with the ethanol of weight percent concentration 47% as solvent, heating and refluxing extraction 16 times is carried every time
The time is taken for 0.3 hour, each solvent load is 44 times of dregs of a decoction A weight, and filtration obtains dregs of a decoction B and extract solution B, and extract solution B is returned
Ethanol is received, relative density 1.14 is concentrated into, filtered, liquid is first washed with water, then use by LSD-280 large pore resin absorption columns
The ethanol solution of weight percent concentration 63% elutes LSD-280 large pore resin absorption columns, collects the ethanol of weight percent concentration 63%
Eluent, reclaims ethanol, and concentrate drying obtains final product extract B；
（3）Extract A and extract B are mixed, pharmaceutical composition is obtained final product.
7. a kind of preparation method of the pharmaceutical composition for treating diabetic retinopathy, its feature according to claim 5
It is that pharmaceutical composition can prepare piece agent or capsule or dripping pill using the conventional method of galenic pharmacy.
8. a kind of preparation method of the pharmaceutical composition for treating diabetic retinopathy, its feature according to claim 5
It is pharmaceutical composition and chemical drugs or Chinese medicine composition treatment diabetic retinopathy medicine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611133161.3A CN106728476A (en) | 2016-12-10 | 2016-12-10 | Treat the pharmaceutical composition of diabetic retinopathy |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611133161.3A CN106728476A (en) | 2016-12-10 | 2016-12-10 | Treat the pharmaceutical composition of diabetic retinopathy |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106728476A true CN106728476A (en) | 2017-05-31 |
Family
ID=58875141
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611133161.3A Withdrawn CN106728476A (en) | 2016-12-10 | 2016-12-10 | Treat the pharmaceutical composition of diabetic retinopathy |
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| Country | Link |
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| CN (1) | CN106728476A (en) |
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2016
- 2016-12-10 CN CN201611133161.3A patent/CN106728476A/en not_active Withdrawn
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Application publication date: 20170531 |
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