CN107535398A - By controlling concentration of formaldehyde to improve Eriocheir hepuensis breeding survival rate and the method for taking the photograph bait amount - Google Patents
By controlling concentration of formaldehyde to improve Eriocheir hepuensis breeding survival rate and the method for taking the photograph bait amount Download PDFInfo
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Abstract
The invention belongs to Hepu suede huge legendary turtle crab cultural technique field, discloses a kind of method by controlling concentration of formaldehyde to improve Eriocheir hepuensis breeding survival rate and take the photograph bait amount, formaldehyde is 87.3580ml/L to the safe concentration of Eriocheir hepuensis；In 12h, 24h, 36h, 48h, 60h, 72h, 84h, 96h, LC of the formaldehyde to Hepu suede chela50Respectively 1991.788,1644.522,1273.757,1724.642,1177.963,1147.138,963.129,873.580.It is contemplated that can be improved by Eriocheir hepuensis and take the photograph bait amount and breeding survival rate for the toxicity data of Hepu suede huge legendary turtle crab by formaldehyde.
Description
Technical field
The invention belongs to Hepu suede huge legendary turtle crab cultural technique field, more particularly to one kind is by controlling concentration of formaldehyde to improve Hepu
Eriocheir breeding survival rate and the method for taking the photograph bait amount.
Background technology
Eriocheir hepuensis (EriocheirHepuensiens) is commonly called as Lithodes, crab, and segmental appendage is subordinate in Zootaxy
Animal door, Crustachia, Decapoda, Grapsidae, suede huge legendary turtle crab category.Suede huge legendary turtle crab is found for 1989 in Guangxi Nan Liujiang for Hepu, due to
Between Eriocheir sinensis and Japanese suede huge legendary turtle crab, Chinese Academy of Sciences animal wears love cloud in 1994 for its young and adult morphological characters
Year is named as Hepu suede huge legendary turtle crab, thinks Eriocheir hepuensis for an independent kind Hepu suede chela through morphologic discriminant analysis
Crab.Once it is found that with its excellent quality, abundant nutrition, unique flavor, growth is fast, growth cycle is short the advantages that and by
To the favor of consumers in general, the producer, turn into the famous-brand and high-quality breed variety of the distinctive aquatic products in Guangxi.Into crab body again be generally 100~
150g, maximum individual reach 450g.In recent years, because the water pollution of getting worse and fishing are excessively captured, Eriocheir hepuensis resource is drastically
Decline.Preferably to protect and developing this precious resources, the research manually developed is extremely urgent.Eriocheir hepuensis
So far do not propagated artificially, the Eriocheir hepuensis that in the market is sold substantially is all from field fishing, and annual amount of fishing is big
It is the distinctive economic seed of south China about in 250~300t, Eriocheir hepuensis, its delicious flavour, fine and tender taste is nutritious,
Individual big, current amount of fishing can not meet the demand in market, cause the Eriocheir hepuensis market price high, manually
Cultivate extremely urgent.The feeding habits of Eriocheir hepuensis are very wide, the equal edible of plant, animal feed, typically main in zoea stage
It is food with unicellular alga, wheel animalcule, artemia, nauplius etc., young crab, into crab then eating fish, shrimp, shellfish, spiral shell and pasture and water, food plaque
Can also torture reactive volt-ampere hour it is similar, to corruption spoil cherish a special interest.In natural environment, Eriocheir hepuensis obtains vegetalitas
Bait comes easy than obtaining animal bait, and therefore, plant food (being mostly pasture and water) often accounts for main component in crab stomach.Close
Pu Eriocheir is heavy, has the digestion of an ostrich, nutriment unnecessary on a full stomach is stored in liver, therefore Eriocheir hepuensis bears hungry ability very
By force, not feeding within 1 month will not also die of hunger.Eriocheir hepuensis is liked burrowing and occupying, and it has the characteristic for autotomying regeneration, when Hepu suede chela
When crab is stimulated by external environment, the foot of a chela can fracture between base pitch and seat section automatically, then automatic growth again, but smaller than original
A lot.The growth course of Eriocheir hepuensis is accompanied by young husking, young young crab or carried out into crab shell, often slough off 1 shell it
Body is long, body weight makees 1 rapid increase.Eriocheir hepuensis at least needs tens of times of shell in life.Eriocheir hepuensis
Live, grow in fresh water, sexual gland can only be developed to IV phase.By cultivating and observing, the paedomorphosis of Eriocheir hepuensis is divided into
Zoea larva and the age in autumn of megalops larva two benches one can grow up to and reach sexal maturity, and sexual gland connects maturescent Eriocheir hepuensis then
Lower progress reproduction to be wandered trip along river, be mated, laid eggs into Jiangkou or shallow sea (formaldehyde 8~33,8~20 DEG C of water temperature), flea
The shape young is drifted about 1 month in river mouth shallow sea, through 5 huskings into megalops larva, is then returned in fresh water and is inhabited growth.It is general to close
The age of Pu Eriocheir 1 individual reaches sexal maturity, and body weight is 10~200g, and minimum sexal maturity individual only has 20~30g.Close at present
Related data, behavioral study in Eriocheir hepuensis is fewer, is to cause Eriocheir hepuensis to cultivate success rate than relatively low, this is
The main reason for currently without extensive artificial farming.Artificial farming is following trend, improves cultivation success rate and cultivation is imitated
Benefit, the aquaculture organism ecological study for carrying out system are necessary.Wherein, in aquaculture, formaldehyde is sterilized as a kind of
It is widely used with the medicine of antiparasitic, formaldehyde is also formalin, and formalin is the aqueous solution containing 40% formaldehyde, spinosity
Swash property stink.Its principle is by alkylated reaction, makes proteinacious, causes the function of enzyme and nucleic acid etc. to change and then reaches
To the purpose of disinfection.Alkylation refers to the hydrogen atom being connected in organic compound molecule on carbon, oxygen and nitrogen and substituted by alkyl
Reaction.Therefore, formalin has the function that powerful killing bacterium, is handled using the formalin of formaldehyde to cold water fish
Species has the effect of being used as bactericide；Formaldehyde exists in addition to each phase zoea larva of Eriocheir japonica sinensis and the acute toxicity of hidden algae
The respectful influence for also having further investigation formalin to Macrobrachium rosenbergii larvae of moral more than in terms of Macrobrachium rosenbergii larvae, formaldehyde is toxic, but
To be current formaldehyde survive to Eriocheir hepuensis and takes the photograph the influence of bait, both at home and abroad without finding report.
In summary, the problem of prior art is present be：Formaldehyde is survived and taken the photograph the influence of bait to Eriocheir hepuensis at present, state
It is inside and outside to be reported without discovery, cause Eriocheir hepuensis to cultivate success rate than relatively low.
The content of the invention
The problem of existing for prior art, the invention provides one kind by controlling concentration of formaldehyde to improve Eriocheir hepuensis
Breeding survival rate and the method for taking the photograph bait amount.
The present invention is achieved in that a kind of by controlling concentration of formaldehyde to improve Eriocheir hepuensis breeding survival rate and taking the photograph bait
The method of amount, it is described to be included by controlling concentration of formaldehyde to improve Eriocheir hepuensis breeding survival rate with the method for taking the photograph bait amount：
Formaldehyde is 87.3580ml/L to the safe concentration of Eriocheir hepuensis；
In 12h, 24h, 36h, 48h, 60h, 72h, 84h, 96h, LC of the formaldehyde to Hepu suede chela50Respectively 1991.788,
1644.522、1273.757、1724.642、1177.963、1147.138、963.129、873.580。
Further, it is described by controlling concentration of formaldehyde to improve Eriocheir hepuensis breeding survival rate and taking the photograph the method use of bait amount
Formaldehyde toxicity is tested and carries out variance analysis using SPSS 17.0.
Further, it is described to be deposited by controlling concentration of formaldehyde to improve Eriocheir hepuensis breeding survival rate and take the photograph the method for bait amount
Motility rate calculation formula：
S=a/b；
S：Survival rate；a：Survived in each container young crab number；b：Each container children crab sum；
Time-to-live calculation formula：
H：Time-to-live；T：Different time sections；a：Young crab viable count in each container；x：Each container children crab mortality；
h：The time-to-live of dead young crab；
It is as follows to take the photograph bait amount calculation formula relatively：
F=D/W × 100%；
F：It is relative to take the photograph bait amount；D：Revised day takes the photograph bait amount；W：Crab body weight.
Further, the first by controlling concentration of formaldehyde raising Eriocheir hepuensis breeding survival rate and the method for taking the photograph bait amount
Aldehyde is to the virulence regression equation of Hepu suede chela：
12h y=10.47-0.05x；
24h y=5.997-0.04x；
36h y=6.477-0.05x；
48h y=2.748-0.02x；
60h y=3.273-0.03x；
72h y=3.558-0.03x；
84h y=4.259-0.04x；
96h y=3.113-0.04x.
Advantages of the present invention and good effect are：With the Eriocheir hepuensis that specification is 16 grams or so, formaldehyde is carried out to Hepu
The examination of suede chela acute toxicity is studied carefully, and as a result finds：Within 48h, concentration of formaldehyde be below 500mg/L to the survival rate of Eriocheir hepuensis and
Time-to-live does not influence (p ＜ 0.05)；Within 96h, below 250mg/L does not have to the survival rate of Eriocheir hepuensis and time-to-live
(p ＜ 0.05) is had a significant impact, in 12h, 24h, 36h, 48h, 60h, 72h, 84h, 96h, LC of the formaldehyde to Hepu suede chela50Respectively
For 1991.788,1644.522,1273.757,1724.642,1177.963,1147.138,963.129,873.580, work as first
During aldehyde concentration cataclysm 96h, LC50Concentration is 87.3580ml/L, and formaldehyde is 87.3580ml/L to the safe concentration of Eriocheir hepuensis.
It is contemplated that by formaldehyde to the toxic effect of Hepu suede huge legendary turtle crab, for the water treatment in the cultivation work of Hepu suede huge legendary turtle crab, disease
Enemy preventing and treating, improve survival rate offer theoretical foundation and actual reference.
Brief description of the drawings
Fig. 1 is provided in an embodiment of the present invention by controlling concentration of formaldehyde to improve Eriocheir hepuensis breeding survival rate and taking the photograph bait
The method flow diagram of amount.
Fig. 2 is influence schematic diagram of the formaldehyde provided in an embodiment of the present invention to Eriocheir hepuensis survival rate.
Fig. 3 is LC of the formaldehyde provided in an embodiment of the present invention to Eriocheir hepuensis50Schematic diagram.
Fig. 4 is factor schematic diagram of the formaldehyde provided in an embodiment of the present invention to the Eriocheir hepuensis time-to-live.
Fig. 5 is the influence schematic diagram that formaldehyde provided in an embodiment of the present invention takes the photograph bait amount to Eriocheir hepuensis children crab.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
The application principle of the present invention is explained in detail below in conjunction with the accompanying drawings.
It is as shown in figure 1, provided in an embodiment of the present invention by controlling concentration of formaldehyde to improve Eriocheir hepuensis breeding survival rate
Comprise the following steps with the method for taking the photograph bait amount：
S101：Set 0mg/L (control), 250mg/L, 500mg/L, 750mg/L, 1000mg/L, 1250mg/L,
Totally 8 concentration of formaldehyde gradients, each concentration are formulated by analyzing pure formaldehyde and aeration running water by 1500mg/L, 1750mg/L；
Per concentration gradient, setting 3 is parallel, is each placed in parallel 10 crabs, altogether 240 crabs；
S102：Variance analysis (One-way ANOVA) is carried out using SPSS 17.0 and examines its significance of difference；With LSR methods
Newly again range test (SSR inspections) method (Duncan) carry out Multiple range test between group；p<0.05 is significant difference；With Probit methods
Obtain 48h, 60h, 72h, 84h, 96h LC50Value；
S103：Calculate survival rate, time-to-live meter, take the photograph bait amount relatively.
The application principle of the present invention is further described with reference to experiment.
1st, materials and methods
1.1 material
1.1.1 Eriocheir hepuensis children crab
Experiment crab used is purchased from Qiezhou east wind market, and after buying back, the running water that experiment crab is placed in aeration 24h is temporarily supported
It is complete, energetic that health, appendage are chosen after 3 days, body weight 16g or so individual is done experiment animal.Carry out the acute toxicity of formaldehyde
Experiment.
1.1.2 cultivation water and breeding barrel
Cultivation container is 46 × 30 × 29cm, and capacity is 50L plastic square barrels, adds the depth of water to 10cm, and cultivation water is after being aerated
Running water, it is standby after being aerated 24h.
1.1.3 bait
Experiment bait is new Fresh squid's meat, purchased from Qiezhou east wind market.
1.1.4 formaldehyde
Formaldehyde (HCHO) [analysis is pure], colourless transparent liquid, has penetrating odor, corrosivity is strong, and molecular mass is
30.03 2500ml/ bottles.Xilong Chemical Co., Ltd produces, drug batch number 1408112.
1.1.5 balance
Experiment balance used is Japanese Shimadzu electronic balance TXB622L.
1.2 method
1.2.1 formaldehyde toxicity is tested
After trial test, this experiment set 0mg/L (control), 250mg/L, 500mg/L, 750mg/L, 1000mg/L,
1250mg/L, 1500mg/L, 1750mg/L totally 8 concentration of formaldehyde gradients, each concentration is by analyzing pure formaldehyde and aeration running water
It is formulated.Per concentration gradient, setting 3 is parallel, is each placed in parallel 10 crabs, altogether 240 crabs.
The good concentration of formaldehyde of existing configuration is put into each gradient.At night 21:00 feeds bait, and each gradient daily ration, feeding quantity is
20g.Morning 8:00 taking-up residual bait simultaneously records the weight of residual bait, changes the aeration running water one time 3 of equivalent, is now made up a prescription when changing water every time
Liquid is to ensure that drug concentration is constant.Test duration 4d, continuous charge during experiment, per 12h, observation once, records it and tests crab
Situation and survival rate, time-to-live and take the photograph bait amount.
In this experiment, corresponding bait solubility test is set, to detect bait solubility value under different concentration of formaldehyde, with
Just bait result is taken the photograph in amendment.
1.2.2 statistical analysis
Variance analysis (One-way ANOVA) is carried out using SPSS 17.0 and examines its significance of difference；It is new with LSR methods
Multiple range test (SSR inspections) method (Duncan) carries out Multiple range test between group；p<0.05 is significant difference；Obtained with Probit methods
48h, 60h, 72h, 84h, 96h LC50Value.
1.2.3 computational methods
Survival rate calculation formula：
S=a/b；
S：Survival rate；a：Survived in each container young crab number；b：Each container children crab sum.
Time-to-live calculation formula：
H：Time-to-live；T：Different time sections (i.e. 12h, 24h, 36h, 48h, 60h, 72h, 84h, 96h different time
Section)；a：Young crab viable count in each container；x：Each container children crab mortality；h：The time-to-live of dead young crab；
It is as follows to take the photograph bait amount calculation formula relatively：
F=D/W × 100%；
F：It is relative to take the photograph bait amount；D：Revised day takes the photograph bait amount；W：Crab body weight.
2 results and analysis
Influence of 2.1 formaldehyde to Eriocheir hepuensis survival rate
The formaldehyde different time sections Eriocheir hepuensis children's crab survival rate of table 1
One-way ANOVA statistics is carried out to the data of table 1
Note：In a curve, letter is identical, represents that difference is not notable；Letter differs, and represents significant difference, similarly hereinafter.
As it is clear from fig. 2 that during 12h, below 1750mg/L concentration of formaldehyde group Eriocheir hepuensis survival rate (100%) difference does not show
Write (P>0.05).1750mg/L concentration of formaldehyde groups survival rate is that (93.0%) otherness is notable, (P<0.05) it is because of Hepu suede chela
Crab shelling is unsuccessful to cause death.During 24h, more than 1250mg/L concentration of formaldehyde group Eriocheir hepuensis survival rates (93.33%) with
Control group there were significant differences (P<, but below 1250mg/L concentration of formaldehyde group Eriocheir hepuensis survival rate and control group difference 0.05)
Not significantly (P>0.05).During 36h, below 1250mg/L concentration of formaldehyde group Eriocheir hepuensis survival rate 100%) and control group difference
Not significantly (P>0.05), but 1250mg/L is notable to having differences property of 1500mg/L.During 48h, more than 250mg/L concentration of formaldehyde groups
Eriocheir hepuensis survival rate and control group significant difference (P<0.05), but below 250mg/L concentration of formaldehyde group Eriocheir hepuensis is deposited
Motility rate and the not notable (P of control group difference>0.05).During 60h, more than 1250mg/L concentration of formaldehyde group Eriocheir hepuensis survival rate with
Control group significant difference (P<0.05), but below 1000mg/L concentration of formaldehyde group Eriocheir hepuensis survival rate and control group difference not
Significantly (P>0.05).During 72h, more than 1000mg/L concentration of formaldehyde group Eriocheir hepuensis survival rate and control group significant difference (P<
, but below 1000mg/L concentration of formaldehyde group Eriocheir hepuensis survival rate and control group difference not significantly (P 0.05)>0.05).Extremely
84h, control group and the not notable (P of 250mg/L groups difference>, but 750mg/L concentrations above group and control group significant difference (P 0.05)
<0.05).During 96h, control group and the not notable (P of 250mg/L groups survival rate (being 100%) difference>0.05), but 500mg/L with
Upper concentration group survival rate and control group significant difference (P<0.05).1500mg/L, 1750 concentration group 96h crabs are all dead, survival
Rate is 0.This explanation, during short time formaldehyde exposure within 4d, below 250mg/L concentration of formaldehyde, Eriocheir hepuensis is survived
Influence unobvious.
The Probit analyses that 2.2 formaldehyde influence on Eriocheir hepuensis
By Fig. 2 data, Probit analyses are carried out, obtain toxicity regression side of the formaldehyde under different time to Hepu suede chela
Journey, it the results are shown in Table 2.
Virulence regression equation of the formaldehyde of table 2 to Eriocheir hepuensis
After Fig. 2 data are carried out into Probit analyses, the LC of obtained formaldehyde to Eriocheir hepuensis50Such as Fig. 3.
It can be seen from figure 3 that over time, formaldehyde is to Eriocheir hepuensis LC50Value gradually reduces.After 48h, it is down to
1724.642mg/L when following, LC50Value gradually tends towards stability.96h, LC50For 873.59mg/L.When concentration is more than 87.359,
The survival rate of Eriocheir hepuensis children crab can be had an impact.So formaldehyde is to the safe concentration of Eriocheir hepuensis children crab
87.359mg/L。
Influence of 2.3 formaldehyde to the Eriocheir hepuensis time-to-live
By influence of the formaldehyde to the time-to-live of Eriocheir hepuensis, 3 are the results are shown in Table.
The time-to-live of the formaldehyde different time sections Eriocheir hepuensis children crab of table 3
96h when calculating off-test is the time-to-live of surviving individual, and 96h acute toxicity test is found, formaldehyde
The time-to-live of Eriocheir hepuensis is influenceed substantially, the data progress One-way ANOVA statistic analysis results to table 3 are shown in Fig. 4.
As seen from Figure 4, below 750mg/L concentration of formaldehyde group, it is poor with control group during the acute toxicity test of 96h times
Different not significantly (P>0.05), but 750mg/L concentrations above groups, the time-to-live is less than 90.866, with control group significant difference (F (6,
17)=1, P<0.05), the 96h times are with compareing 1750mg/L concentration of formaldehyde groups, during 96h acute toxicity test, during survival
Between be only 46.3h.
2.4 formaldehyde take the photograph the influence of bait to Eriocheir hepuensis
Formaldehyde takes the photograph the influence of bait to Eriocheir hepuensis, the results are shown in Table 4.
The formaldehyde different time sections Eriocheir hepuensis of table 4 takes the photograph bait amount
The data of table 4 are subjected to statistical analysis, as a result see Fig. 5.
From figure 5 it can be seen that formaldehyde cataclysm influences significantly on the bait of taking the photograph of Eriocheir hepuensis in cataclysm 24h：Concentration of formaldehyde
Below 500mg/L group differences are not significantly (p ＞ 0.05), but notable with concentration of formaldehyde 500mg/L above group differences.Cataclysm
During 48h, formaldehyde makes a significant impact on the bait amount of taking the photograph of Eriocheir hepuensis：The following group differences of cataclysm formaldehyde 500mg/L are not notable
(p ＞ 0.05), not significantly (p ＞ 0.05), during cataclysm 72h, formaldehyde is to Hepu for concentration of formaldehyde cataclysm 500mg/L above group difference
The bait amount of taking the photograph of Eriocheir makes a significant impact：Not significantly (p ＞ 0.05), formaldehyde is dense for the following group differences of cataclysm formaldehyde 500mg/L
Spend cataclysm 500mg/L above group difference not significantly (p ＞ 0.05) cataclysm 96h when, formaldehyde cataclysm concentration is to Eriocheir hepuensis
Bait amount is taken the photograph to make a significant impact (p ＜ 0.05)：Significant difference (p ＜ 0.05) between more than 200mg/L and control group, generally speaking,
Below concentration of formaldehyde cataclysm 200mg/L, the bait amount of taking the photograph of Eriocheir hepuensis is had no significant effect, concentration can shadow more than 200mg/L
That rings Eriocheir hepuensis takes the photograph bait amount.In 4d acute toxicity test, each group concentration group crab takes the photograph bait amount (spy integrally on a declining curve
When being not on-test).But with the adaptation of crab PARA FORMALDEHYDE PRILLS(91,95), low concentration group is taken the photograph bait amount and gradually gone up, such as 1500mg/L groups are taken the photograph
Bait amount rebound significantly (p ＜ 0.05).
3 results are with discussing
Influence of 3.1 formaldehyde to Eriocheir hepuensis survival rate
From the point of view of this result of the test, formaldehyde cataclysm produces significant shadow to the survival rate of Eriocheir hepuensis and time-to-live
Although ringing (p ＜ 0.05), in terms of survival rate, with the rise of concentration of formaldehyde, the survival rate of Eriocheir hepuensis decreases, survival
Time is short, below 500mg/L concentration groups, time-to-live and the not notable (P of control group difference>0.05).Before 84h, 1250mg/L
Concentration group and the not notable (P of control group difference>0.05), but during 96h, 1250mg/L concentration group and control group significant difference (P<
0.05), only 250mg/L concentration group and control group difference not significantly (P>0.05).This explanation, in terms of acute toxicity test,
Below 250mg/L concentration of formaldehyde will not survive to Eriocheir hepuensis to be had undesirable effect.More than 250mg/L concentration of formaldehyde group with it is right
According to a group significant difference, in concentration 1750mg/L, 96h survival rates are 0%.Illustrate to be adapted to the existence concentration of Eriocheir hepuensis probably to exist
250mg/L, to 96h, LC50For 873.580mg/L, formaldehyde is 87.358mg/L to the safe concentration of Eriocheir hepuensis children crab, is said
Bright concentration of formaldehyde is safe concentration in 87.358mg/L, and certainly, this is for acute toxicity test, not equivalent to chronic
Toxicity test.Although 250mg/L concentration group survival rates and time-to-live are not significantly different (P with control group>0.05), but it is taken the photograph
Bait amount is but substantially lower than control group.Although the later stage of acute toxicity test, the bait amount of taking the photograph of 250mg/L concentration group crabs significantly rises (P
<0.05), but still with control group there were significant differences (P<0.05).This just illustrates that 40mg/L concentration of formaldehyde still can be to Eriocheir hepuensis
Generate certain injury therefore 96h LC50For the safe concentration of Eriocheir hepuensis.
3.2 formaldehyde take the photograph the influence of bait to Eriocheir hepuensis children crab
In this experiment, each concentration group takes the photograph bait.Over time, Eriocheir hepuensis day, it is gradual to take the photograph bait amount
Reduce, 250mg/L concentration groups Eriocheir hepuensis before 72h takes the photograph bait amount group difference not significantly (p ＞ 0.05), and 250mg/L is dense
Degree group takes the photograph bait after 72h on young crab to be influenceed significantly (p ＜ 0.05).From taking the photograph in bait amount, taking the photograph bait amount to 96 hours is only
1.6%, illustrate, cataclysm amplitude is too big or too high, and Eriocheir hepuensis is difficult in adapt to, so as to show take the photograph bait amount reduce tail off, even
It is dead.During to 72h, taking the photograph bait amount has obvious rise, illustrates that Eriocheir hepuensis produces adaptability to a certain extent.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement made within refreshing and principle etc., should be included in the scope of the protection.
Claims (4)
- A kind of 1. method by controlling concentration of formaldehyde to improve Eriocheir hepuensis breeding survival rate and take the photograph bait amount, it is characterised in that It is described to be included by controlling concentration of formaldehyde to improve Eriocheir hepuensis breeding survival rate with the method for taking the photograph bait amount：Formaldehyde is 87.3580ml/L to the safe concentration of Eriocheir hepuensis；In 12h, 24h, 36h, 48h, 60h, 72h, 84h, 96h, LC of the formaldehyde to Hepu suede chela50Respectively 1991.788mg/L, 1644.522mg/L、1273.757mg/L、1724.642mg/L、1177.963mg/Lmg/L、1147.138、963.129mg/L、 873.580mg/L。
- 2. as claimed in claim 1 by controlling concentration of formaldehyde to improve Eriocheir hepuensis breeding survival rate and taking the photograph the side of bait amount Method, it is characterised in that described by controlling concentration of formaldehyde to improve Eriocheir hepuensis breeding survival rate and taking the photograph the method use of bait amount Formaldehyde toxicity is tested and carries out variance analysis using SPSS 17.0.
- 3. as claimed in claim 1 by controlling concentration of formaldehyde to improve Eriocheir hepuensis breeding survival rate and taking the photograph the side of bait amount Method, it is characterised in that described to be deposited by controlling concentration of formaldehyde to improve Eriocheir hepuensis breeding survival rate and take the photograph the method for bait amount Motility rate calculation formula：S=a/b；S：Survival rate；a：Survived in each container young crab number；b：Each container children crab sum；Time-to-live calculation formula：<mrow> <mi>H</mi> <mo>=</mo> <mfrac> <mrow> <mi>T</mi> <mi>a</mi> <mo>+</mo> <mi>x</mi> <mi>h</mi> </mrow> <mn>10</mn> </mfrac> <mo>;</mo> </mrow>H：Time-to-live；T：Different time sections；a：Young crab viable count in each container；x：Each container children crab mortality；h：Extremely Die the time-to-live of young crab；It is as follows to take the photograph bait amount calculation formula relatively：F=D/W × 100%；F：It is relative to take the photograph bait amount；D：Revised day takes the photograph bait amount；W：Crab body weight.
- 4. as claimed in claim 1 by controlling concentration of formaldehyde to improve Eriocheir hepuensis breeding survival rate and taking the photograph the side of bait amount Method, it is characterised in that the first by controlling concentration of formaldehyde raising Eriocheir hepuensis breeding survival rate and the method for taking the photograph bait amount Aldehyde is to the virulence regression equation of Hepu suede chela：12h y=10.47-0.05x；24h y=5.997-0.04x；36h y=6.477-0.05x；48h y=2.748-0.02x；60h y=3.273-0.03x；72h y=3.558-0.03x；84h y=4.259-0.04x；96h y=3.113-0.04x.
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| CN101617638A (en) * | 2008-07-04 | 2010-01-06 | 中国科学院海洋研究所 | Construction method of portunus trituberculatus miers disease-resistant variety |
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| CN101617638A (en) * | 2008-07-04 | 2010-01-06 | 中国科学院海洋研究所 | Construction method of portunus trituberculatus miers disease-resistant variety |
Non-Patent Citations (4)
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| 何碧华: "氨氮对红星梭子蟹早期幼体的急性毒性", 《水产科学》 * |
| 廖永岩: "氨氮对合浦绒鳌蟹的急性毒性试验", 《淡水渔业》 * |
| 沈和定: "甲醛对中华绒鳌蟹各期溞状幼体及隐藻的急性毒性", 《上海水产大学学报》 * |
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