CN107523027A - A kind of preparation method of the degradable antimicrobial preservative film of longan seed - Google Patents
A kind of preparation method of the degradable antimicrobial preservative film of longan seed Download PDFInfo
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- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L67/00—Compositions of polyesters obtained by reactions forming a carboxylic ester link in the main chain; Compositions of derivatives of such polymers
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- C08J5/00—Manufacture of articles or shaped materials containing macromolecular substances
- C08J5/18—Manufacture of films or sheets
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2367/00—Characterised by the use of polyesters obtained by reactions forming a carboxylic ester link in the main chain; Derivatives of such polymers
- C08J2367/04—Polyesters derived from hydroxy carboxylic acids, e.g. lactones
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- C—CHEMISTRY; METALLURGY
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- C08J2403/00—Characterised by the use of starch, amylose or amylopectin or of their derivatives or degradation products
- C08J2403/02—Starch; Degradation products thereof, e.g. dextrin
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- C08L2205/00—Polymer mixtures characterised by other features
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Abstract
The present invention provides a kind of preparation method of the degradable antimicrobial preservative film of longan seed, belongs to fresh-keeping technical field of membrane, comprises the following steps：Longan seed is broken into pulpous state, gained slurry is sieved, alpha amylase is added into gained filter residue to be digested, gained mixed liquor is filtered and centrifuged, cellulase degradation is added into supernatant and obtains enzymolysis product, enzymolysis product is put into more stainless steel extractors, extracts to obtain longan seed leaching liquor using subcritical extraction method；The filtrate of longan seed is extracted into longan seed starch for 89 in pH value with reference to supersound process；The materials such as longan seed leaching liquor, longan seed starch pectin combination soybean protein isolate, PLA, chitosan, silica, water are put into extruder, preservative film is extruded at a temperature of 140 145 DEG C；Wrap film safety obtained by the present invention is nontoxic, can reach the tensile strength and elongation at break of food fresh keeping membrane, while has the advantages of degradation rate is high, and degradation cycle is short.
Description
【Technical field】
The present invention relates to fresh-keeping technical field of membrane, the preparation method of especially a kind of degradable antimicrobial preservative film of longan seed.
【Technical background】
Containing abundant glucose, sucrose and protein etc. in longan, iron-holder is also higher, can improve heat energy, supplement
Promote hemoglobin regeneration while nutrition, so as to reach the effect enriched blood, therefore physically weak weak, anaemia is suffered from after women childbirth
Person and the patient of person in middle and old age's insufficiency of vital energy and blood also are adapted for taking longan.
For longan in addition to longan pulp has higher nutritive value, its seed also has remarkable efficacy and application value；
Containing a variety of natural components such as abundant starch, reduced sugar, protein, pectin, crude fibres in longan seed, while also containing a variety of
The nutrients such as mineral element, soap leather element, wherein, content of starch 60.88%, content of reducing sugar 9.62%, crude fiber content
For 6.86%, protein content 5.58%, pectin content 5.18%.Due to longan seed starch it is nontoxic, it is hydrophilic, have stick
Property, biocompatibility and biological degradability.
It is one of mainstream technology of Recent study using degradable preservative film at present, this preservative film not only really can mistake
Extend storage phase, fresh-keeping effect is high, and existing preservative film is prepared using polyvinyl alcohol with starch, then using preservative film to vegetable
Fruit is packed, and reaches fresh-keeping effect, but its fresh-keeping effect is bad, and does not have antibiotic property；However as people
The worry of the environmental protection concept that increasingly strengthens and consumer for the potential safety hazard of some toxic preservative films, it is impossible to which satisfaction disappears now
Take the demand of crowd.
【The content of the invention】
In view of posed problems above, the invention provides a kind of preparation method of the degradable antimicrobial preservative film of longan seed,
Resulting Wrap film safety is nontoxic, can reach the tensile strength and elongation at break of food fresh keeping membrane, while has degradation rate
Height, the advantages of degradation cycle is short.
Technical scheme is as follows：
A kind of preparation method of the degradable antimicrobial preservative film of longan seed, comprises the following steps：
(1) longan seed is cleaned and crushes, breaks into pulpous state, gained slurry is crossed into 100-120 mesh sieves, retain respectively filter residue and
Filtrate is standby；
(2) add 6-8 times of water of its quality into step (1) described filter residue and obtain filter residue mixed liquor, then mixed to filter residue
Alpha-amylase is added in liquid, enzymolysis 2-3h is carried out under the conditions of 70-72 DEG C of temperature, digest end mixed liquor filtered and from
The heart, supernatant is collected, and sterilization treatment is carried out to supernatant；Cellulase is added into supernatant again, in 60-65 DEG C of constant temperature
In water-bath, after digesting 1-2h, the enzyme deactivation 5-8min at a temperature of 80 DEG C -85 DEG C is taken out, filtrate is then collected after filtering, obtains
Enzymolysis product；
(3) step (2) described enzymolysis product is put into stainless steel extractor, 30- is extracted using subcritical extraction method
35min, collect filter liquor and obtain longan seed leaching liquor；
(4) step (1) the filtrate regulation pH value is placed in be ultrasonically treated in environment to 8-9 and handles 3-5min, stand 20-
Supernatant liquid is discharged after 30min；The pH value of regulation surplus materials is placed in be ultrasonically treated in environment for 8-9 again handles 3-5min,
Supernatant liquid is discharged after standing 20-30min；Dried at a temperature of remaining material is put in into 60-62 DEG C, obtain longan seed starch；
(5) according to parts by weight, pectin 1-2 parts, soybean protein isolate 1-3 parts, PLA 20-40 parts, chitosan 1-8 are chosen
Part, longan seed leaching liquor 2-6 parts, longan seed starch 8-15 parts, silica 0.02-0.08 parts, triethanolamine oleate 0.01-
0.04 part, potassium sorbate 0.1-0.6 parts, acetic acid 0.5-1.8 parts, horse propylene copolymer 1-5 parts, vegetable oil 2-8 parts, furfural 3-8 parts,
Fructose 4-10 parts, water 40-60 parts, plasticizer 1-3 parts, defoamer 0.5-1.5 parts, coupling agent 0.5-1.9 parts；Above-mentioned substance is mixed
Conjunction is uniformly put into extruder, and preservative film is extruded under the conditions of being 140-145 DEG C in temperature.
Further, in step (2), the usage amount of the alpha-amylase is the 0.05- of the filter residue mixed liquor
0.08wt%.
Further, in step (2), the usage amount of the cellulase is the 0.03-0.05wt% of the supernatant.
Further, in step (3), the subcritical abstraction process be in temperature be 105-115 DEG C, pressure 2-
Extracted under conditions of 3MPa.
Further, in step (4), the power of the ultrasonic wave is 300-500W.
Further, in step (5), according to parts by weight, 1.5 parts of pectin, 2 parts of soybean protein isolate, PLA are chosen
30 parts, 6 parts of chitosan, 4 parts of longan seed leaching liquor, 10 parts of longan seed starch, 0.04 part of silica, triethanolamine oleate 0.02
Part, 0.5 part of potassium sorbate, 0.9 part of acetic acid, 3 parts of horse propylene copolymer, 6 parts of vegetable oil, 4 parts of furfural, 6 parts of fructose, 50 parts of water, increase
Mould 2 parts of agent, 0.8 part of defoamer, 1.2 parts of coupling agent.
Further, in step (5), the thickness of the preservative film is 8-10 μm.
The invention provides a kind of preparation method of the degradable antimicrobial preservative film of longan seed, compared with prior art, has
Following beneficial effect：
Longan seed is broken into pulpous state by the present invention first, and gained slurry is sieved, alpha-amylase is added into gained filter residue
Digested, gained mixed liquor is filtered and centrifuged, and cellulase degradation is added into supernatant and obtains enzymolysis product, by institute
Obtain enzymolysis product to be put into stainless steel extractor, extract to obtain longan seed leaching liquor, subcritical extraction using subcritical extraction method
Take has many advantages compared to other separation methods, and its nontoxic, non-thermal technology can retain the active component of extract not by broken
Badly and aoxidize, the things such as mannosan, flavonols glycocide are contained in the longan seed leaching liquor obtained by subcritical extraction method
Matter, there is the effect of suppression is to various bacteria, there is the elasticity of enhancing vegetables and fruits epidermal cell wall and suppress its oxidation, can also protect
The coat color of vegetables and fruits is held, phenomena such as so as to prevent vegetables and fruits fruit from producing weightless, shrinkage, the outward appearance of vegetables and fruits is maintained, further carries
Its high fresh-keeping effect；It is 8-9 that the filtrate of longan seed is adjusted into pH value, and extracts longan seed starch, gained with reference to supersound process
Longan seed starch there is good pliability and ductility；By longan seed leaching liquor, longan seed starch pectin combination soybean point
From albumen, PLA, chitosan, silica, triethanolamine oleate, potassium sorbate, acetic acid, horse propylene copolymer, vegetable oil, chaff
The materials such as aldehyde, fructose, water, plasticizer, defoamer, coupling agent are put into extruder, are extruded at a temperature of 140-145 DEG C fresh-keeping
Film；Wherein, polylactic acid film has good gas permeability, oxygen permeability and saturating carbon dioxide, and it also has the spy of isolation smell
Property, and PLA is that uniquely have excellent antibacterial and anti-mildew characteristic biodegradable plastic, will not have virus or mould according to
The danger on the surface of biodegradable plastic is attached to, and can also improve the tensile strength and extensibility of preservative film, can quilt after use
Microorganism is degradable in nature, ultimately generates carbon dioxide and water, and free from environmental pollution, this is highly beneficial to environmental protection,
It is generally acknowledged environment-friendly material；Each technological means of the present invention all cooperates, mutually promoted, and step by step
For battalion, it is all linked with one another, caused total technique effect is significantly larger than the letter of technological means caused by single technological means
It is single plus and.
In a word, the preparation method of the degradable antimicrobial preservative film of longan seed provided by the invention, resulting Wrap film safety
It is nontoxic, the tensile strength and elongation at break of food fresh keeping membrane are can reach, while have that degradation rate is high, short excellent of degradation cycle
Point.
【Embodiment】
The following examples can help those skilled in the art that the present invention is more completely understood, but cannot be with any
Mode limits the present invention.
Embodiment 1
A kind of preparation method of the degradable antimicrobial preservative film of longan seed, comprises the following steps：
(1) longan seed is cleaned and crushes, breaks into pulpous state, gained slurry is crossed into 100 mesh sieves, retain filter residue and filtrate respectively
It is standby；
(2) 6 times of water of its quality are added into step (1) described filter residue and obtains filter residue mixed liquor, then to filter residue mixed liquor
Middle addition alpha-amylase, carries out enzymolysis 2h under the conditions of temperature 70 C, and the mixed liquor for digesting end is filtered and centrifuged, and collects
Supernatant, and sterilization treatment is carried out to supernatant；Cellulase is added into supernatant again, in 60 DEG C of thermostat water bath,
After digesting 1h, the enzyme deactivation 5min at a temperature of 80 DEG C is taken out, filtrate is then collected after filtering, obtains enzymolysis product；Wherein, it is described
The usage amount of alpha-amylase is the 0.05wt% of the filter residue mixed liquor；The usage amount of the cellulase is the supernatant
0.03wt%；
(3) step (2) described enzymolysis product is put into stainless steel extractor, extracted using subcritical extraction method
30min, collect filter liquor and obtain longan seed leaching liquor；Wherein, the subcritical abstraction process is 105 DEG C, pressed in temperature
Power is extracted under conditions of being 2MPa；
(4) step (1) filtrate is adjusted into pH value to 8, is placed in supersound process environment and handles 3min, stand 20min
After discharge supernatant liquid；The pH value for adjusting surplus materials again is 8, is placed in supersound process environment and handles 3min, stands 20min
After discharge supernatant liquid；Remaining material is put at a temperature of 60 DEG C and dried, obtains longan seed starch；Wherein, the ultrasonic wave
Power is 300W；
(5) according to parts by weight, 1 part of pectin, 1 part of soybean protein isolate, 20 parts of PLA, 1 part of chitosan, longan seed are chosen
2 parts of leaching liquor, 8 parts of longan seed starch, 0.02 part of silica, 0.01 part of triethanolamine oleate, 0.1 part of potassium sorbate, acetic acid
0.5 part, it is 1 part of horse propylene copolymer, 2 parts of vegetable oil, 3 parts of furfural, 4 parts of fructose, 40 parts of water, 1 part of plasticizer, 0.5 part of defoamer, even
Join 0.5 part of agent；Above-mentioned substance is well mixed and is put into extruder, preservative film is extruded under the conditions of being 140 DEG C in temperature；Wherein,
The thickness of the preservative film is 8 μm.
Embodiment 2
A kind of preparation method of the degradable antimicrobial preservative film of longan seed, comprises the following steps：
(1) longan seed is cleaned and crushes, breaks into pulpous state, gained slurry is crossed into 120 mesh sieves, retain filter residue and filtrate respectively
It is standby；
(2) 8 times of water of its quality are added into step (1) described filter residue and obtains filter residue mixed liquor, then to filter residue mixed liquor
Middle addition alpha-amylase, enzymolysis 3h is carried out under the conditions of 72 DEG C of temperature, the mixed liquor for digesting end is filtered and centrifuged, and is collected
Supernatant, and sterilization treatment is carried out to supernatant；Cellulase is added into supernatant again, in 65 DEG C of thermostat water bath,
After digesting 2h, the enzyme deactivation 8min at a temperature of 85 DEG C is taken out, filtrate is then collected after filtering, obtains enzymolysis product；Wherein, it is described
The usage amount of alpha-amylase is the 0.08wt% of the filter residue mixed liquor；The usage amount of the cellulase is the supernatant
0.05wt%；
(3) step (2) described enzymolysis product is put into stainless steel extractor, extracted using subcritical extraction method
35min, collect filter liquor and obtain longan seed leaching liquor；Wherein, the subcritical abstraction process is 115 DEG C, pressed in temperature
Power is extracted under conditions of being 3MPa；
(4) step (1) filtrate is adjusted into pH value to 9, is placed in supersound process environment and handles 5min, stand 30min
After discharge supernatant liquid；The pH value for adjusting surplus materials again is 9, is placed in supersound process environment and handles 5min, stands 30min
After discharge supernatant liquid；Remaining material is put at a temperature of 62 DEG C and dried, obtains longan seed starch；Wherein, the ultrasonic wave
Power is 500W；
(5) according to parts by weight, 2 parts of pectin, 3 parts of soybean protein isolate, 40 parts of PLA, 8 parts of chitosan, longan seed are chosen
6 parts of leaching liquor, 15 parts of longan seed starch, 0.08 part of silica, 0.04 part of triethanolamine oleate, 0.6 part of potassium sorbate, acetic acid
1.8 parts, 5 parts of horse propylene copolymer, 8 parts of vegetable oil, 8 parts of furfural, 10 parts of fructose, 60 parts of water, 3 parts of plasticizer, 1.5 parts of defoamer,
1.9 parts of coupling agent；Above-mentioned substance is well mixed and is put into extruder, preservative film is extruded under the conditions of being 145 DEG C in temperature；Its
In, the thickness of the preservative film is 10 μm.
Embodiment 3
A kind of preparation method of the degradable antimicrobial preservative film of longan seed, comprises the following steps：
(1) longan seed is cleaned and crushes, breaks into pulpous state, gained slurry is crossed into 110 mesh sieves, retain filter residue and filtrate respectively
It is standby；
(2) 7 times of water of its quality are added into step (1) described filter residue and obtains filter residue mixed liquor, then to filter residue mixed liquor
Middle addition alpha-amylase, enzymolysis 2.5h is carried out under the conditions of 71 DEG C of temperature, the mixed liquor for digesting end is filtered and centrifuged, and is received
Collect supernatant, and sterilization treatment is carried out to supernatant；Cellulase is added into supernatant again, in 62 DEG C of thermostat water bath
In, after digesting 1.5h, the enzyme deactivation 6min at a temperature of 82 DEG C is taken out, filtrate is then collected after filtering, obtains enzymolysis product；Its
In, the usage amount of the alpha-amylase is the 0.06wt% of the filter residue mixed liquor；The usage amount of the cellulase is described
The 0.04wt% of supernatant；
(3) step (2) described enzymolysis product is put into stainless steel extractor, extracted using subcritical extraction method
32min, collect filter liquor and obtain longan seed leaching liquor；Wherein, the subcritical abstraction process is 108 DEG C, pressed in temperature
Power is extracted under conditions of being 2.5MPa；
(4) step (1) filtrate is adjusted into pH value to 8, is placed in handling 4min in supersound process environment, stands
Supernatant liquid is discharged after 25min；The pH value of regulation surplus materials is placed in be ultrasonically treated in environment for 9 again handles 4min, stands
Supernatant liquid is discharged after 25min；Remaining material is put at a temperature of 61 DEG C and dried, obtains longan seed starch；Wherein, the ultrasound
The power of ripple is 400W；
(5) according to parts by weight, 1.5 parts of pectin, 2 parts of soybean protein isolate, 30 parts of PLA, 6 parts of chitosan, longan are chosen
4 parts of core leaching liquor, 10 parts of longan seed starch, 0.04 part of silica, 0.02 part of triethanolamine oleate, 0.5 part of potassium sorbate, second
Acid 0.9 part, 3 parts of horse propylene copolymer, 6 parts of vegetable oil, 4 parts of furfural, 6 parts of fructose, 50 parts of water, 2 parts of plasticizer, 0.8 part of defoamer,
1.2 parts of coupling agent；Above-mentioned substance is well mixed and is put into extruder, preservative film is extruded under the conditions of being 142 DEG C in temperature；Its
In, the thickness of the preservative film is 9 μm.
Comparative example 1
A kind of preparation method of the degradable antimicrobial preservative film of longan seed, comprises the following steps：
(1) longan seed is cleaned and crushes, breaks into pulpous state, gained slurry is crossed into 110 mesh sieves, retain filter residue and filtrate respectively
It is standby；
(2) 7 times of water of its quality are added into step (1) described filter residue and obtains filter residue mixed liquor, then to filter residue mixed liquor
Middle addition alpha-amylase, enzymolysis 2.5h is carried out under the conditions of 71 DEG C of temperature, the mixed liquor for digesting end is filtered and centrifuged, and is received
Collect supernatant, obtain enzymolysis product；Wherein, the usage amount of the alpha-amylase is the 0.06wt% of the filter residue mixed liquor；
(3) step (2) described enzymolysis product is put into stainless steel extractor, extracted using subcritical extraction method
32min, collect filter liquor and obtain longan seed leaching liquor；Wherein, the subcritical abstraction process is 108 DEG C, pressed in temperature
Power is extracted under conditions of being 2.5MPa；
(4) step (1) filtrate is adjusted into pH value to 8, is placed in handling 4min in supersound process environment, stands
Supernatant liquid is discharged after 25min；The pH value of regulation surplus materials is placed in be ultrasonically treated in environment for 9 again handles 4min, stands
Supernatant liquid is discharged after 25min；Remaining material is put at a temperature of 61 DEG C and dried, obtains longan seed starch；Wherein, the ultrasound
The power of ripple is 400W；
(5) according to parts by weight, 1.5 parts of pectin, 2 parts of soybean protein isolate, 30 parts of PLA, 6 parts of chitosan, longan are chosen
4 parts of core leaching liquor, 10 parts of longan seed starch, 0.04 part of silica, 0.02 part of triethanolamine oleate, 0.5 part of potassium sorbate, second
Acid 0.9 part, 3 parts of horse propylene copolymer, 6 parts of vegetable oil, 4 parts of furfural, 6 parts of fructose, 50 parts of water, 2 parts of plasticizer, 0.8 part of defoamer,
1.2 parts of coupling agent；Above-mentioned substance is well mixed and is put into extruder, preservative film is extruded under the conditions of being 142 DEG C in temperature；Its
In, the thickness of the preservative film is 9 μm.
Comparative example 2
A kind of preparation method of the degradable antimicrobial preservative film of longan seed, comprises the following steps：
(1) longan seed is cleaned and crushes, breaks into pulpous state, gained slurry is crossed into 110 mesh sieves, retain filter residue and filtrate respectively
It is standby；
(2) 7 times of water of its quality are added into step (1) described filter residue and obtains filter residue mixed liquor, then to filter residue mixed liquor
Middle addition alpha-amylase, enzymolysis 2.5h is carried out under the conditions of 71 DEG C of temperature, the mixed liquor for digesting end is filtered and centrifuged, and is received
Collect supernatant, and sterilization treatment is carried out to supernatant；Cellulase is added into supernatant again, in 62 DEG C of thermostat water bath
In, after digesting 1.5h, the enzyme deactivation 6min at a temperature of 82 DEG C is taken out, then collects filtrate after filtering, obtains longan seed leaching liquor；
Wherein, the usage amount of the alpha-amylase is the 0.06wt% of the filter residue mixed liquor；The usage amount of the cellulase is institute
State the 0.04wt% of supernatant；
(3) step (1) filtrate is adjusted into pH value to 8, is placed in handling 4min in supersound process environment, stands
Supernatant liquid is discharged after 25min；The pH value of regulation surplus materials is placed in be ultrasonically treated in environment for 9 again handles 4min, stands
Supernatant liquid is discharged after 25min；Remaining material is put at a temperature of 61 DEG C and dried, obtains longan seed starch；Wherein, the ultrasound
The power of ripple is 400W；
(4) according to parts by weight, 1.5 parts of pectin, 2 parts of soybean protein isolate, 30 parts of PLA, 6 parts of chitosan, longan are chosen
4 parts of core leaching liquor, 10 parts of longan seed starch, 0.04 part of silica, 0.02 part of triethanolamine oleate, 0.5 part of potassium sorbate, second
Acid 0.9 part, 3 parts of horse propylene copolymer, 6 parts of vegetable oil, 4 parts of furfural, 6 parts of fructose, 50 parts of water, 2 parts of plasticizer, 0.8 part of defoamer,
1.2 parts of coupling agent；Above-mentioned substance is well mixed and is put into extruder, preservative film is extruded under the conditions of being 142 DEG C in temperature；Its
In, the thickness of the preservative film is 9 μm.
Comparative example 3
A kind of preparation method of the degradable antimicrobial preservative film of longan seed, comprises the following steps：
(1) longan seed is cleaned and crushes, breaks into pulpous state, gained slurry is crossed into 110 mesh sieves, retain filter residue and filtrate respectively
It is standby；
(2) 7 times of water of its quality are added into step (1) described filter residue and obtains filter residue mixed liquor, then to filter residue mixed liquor
Middle addition alpha-amylase, enzymolysis 2.5h is carried out under the conditions of 71 DEG C of temperature, the mixed liquor for digesting end is filtered and centrifuged, and is received
Collect supernatant, and sterilization treatment is carried out to supernatant；Cellulase is added into supernatant again, in 62 DEG C of thermostat water bath
In, after digesting 1.5h, the enzyme deactivation 6min at a temperature of 82 DEG C is taken out, filtrate is then collected after filtering, obtains enzymolysis product；Its
In, the usage amount of the alpha-amylase is the 0.06wt% of the filter residue mixed liquor；The usage amount of the cellulase is described
The 0.04wt% of supernatant；
(3) step (2) described enzymolysis product is put into stainless steel extractor, extracted using subcritical extraction method
32min, collect filter liquor and obtain longan seed leaching liquor；Wherein, the subcritical abstraction process is 108 DEG C, pressed in temperature
Power is extracted under conditions of being 2.5MPa；
(4) step (1) filtrate is adjusted into pH value to 8, supernatant liquid is discharged after standing 25min；Remaining material is put
Dried at a temperature of 61 DEG C, obtain longan seed starch；
(5) according to parts by weight, 1.5 parts of pectin, 2 parts of soybean protein isolate, 30 parts of PLA, 6 parts of chitosan, longan are chosen
4 parts of core leaching liquor, 10 parts of longan seed starch, 0.04 part of silica, 0.02 part of triethanolamine oleate, 0.5 part of potassium sorbate, second
Acid 0.9 part, 3 parts of horse propylene copolymer, 6 parts of vegetable oil, 4 parts of furfural, 6 parts of fructose, 50 parts of water, 2 parts of plasticizer, 0.8 part of defoamer,
1.2 parts of coupling agent；Above-mentioned substance is well mixed and is put into extruder, preservative film is extruded under the conditions of being 142 DEG C in temperature；Its
In, the thickness of the preservative film is 9 μm.
Comparative example 4
A kind of preparation method of the degradable antimicrobial preservative film of longan seed, comprises the following steps：
(1) longan seed is cleaned and crushes, breaks into pulpous state, gained slurry is crossed into 110 mesh sieves, it is standby to retain filtrate respectively；
(2) step (1) filtrate is adjusted into pH value to 8, is placed in handling 4min in supersound process environment, stands
Supernatant liquid is discharged after 25min；The pH value of regulation surplus materials is placed in be ultrasonically treated in environment for 9 again handles 4min, stands
Supernatant liquid is discharged after 25min；Remaining material is put at a temperature of 61 DEG C and dried, obtains longan seed starch；Wherein, the ultrasound
The power of ripple is 400W；
(3) according to parts by weight, 1.5 parts of pectin, 2 parts of soybean protein isolate, 30 parts of PLA, 6 parts of chitosan, longan are chosen
10 parts of core starch, 0.04 part of silica, 0.02 part of triethanolamine oleate, 0.5 part of potassium sorbate, 0.9 part of acetic acid, horse third are copolymerized
3 parts of thing, 6 parts of vegetable oil, 4 parts of furfural, 6 parts of fructose, 50 parts of water, 2 parts of plasticizer, 0.8 part of defoamer, 1.2 parts of coupling agent；Will be upper
State material mixing to be uniformly put into extruder, preservative film is extruded under the conditions of being 142 DEG C in temperature；Wherein, the thickness of the preservative film
Spend for 9 μm.
Experimental example
Experiment is divided into 7 groups, and gained preservative film is used to protect by each group according to embodiment 1-3 and comparative example 1-4 preparation method
Fresh 50 apples, observation, record preserve two months fruit brownings, mildew situation, and standards of grading are shown in Table 1, and appraisal result is shown in Table 2.
The fresh-keeping effect standards of grading of the preservative film of table 1
| Evaluation criterion | Score |
| Fresh intact, no foxiness or waterlogging spot, or speck area are less than 1/4, and flesh color, flavor are normal, non-mildew | One-level fruit |
| Between 1/4-1/2, flesh color, flavor are normal for foxiness or waterlogging spot area and fruit area ratio, non-mildew | Two level fruit |
| Between 1/2-3/4, flesh color, flavor are slightly poor for foxiness or waterlogging spot area and fruit area ratio, slight mildew | Three-level fruit |
| Foxiness or waterlogging spot area are more than 3/4 with fruit area ratio, and flesh color, flavor are poor, mildew | Level Four fruit |
| Fruit rots completely | Pyatyi fruit |
The fresh-keeping effect of the preservative film of table 2
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 | Comparative example 2 | Comparative example 3 | Comparative example 4 | |
| Score | One-level fruit | One-level fruit | One-level fruit | Level Four fruit | Three-level fruit | Level Four fruit | Three-level fruit |
| Browning rate (%) | 0 | 0 | 0 | 82 | 64 | 86 | 54 |
| Weight-loss ratio (%) | 98.8 | 99.3 | 98.5 | 21.6 | 33.5 | 19.8 | 41.5 |
Wherein, the fruit number that generation brown stain and cortex degenerate after browning rate=storage/total fruit number × 100%
Fruit gross mass × 100% before fruit gross mass/storage after weight-loss ratio=storage
The result of table 2 shows：Embodiment 1-3 preservative film is interior during bimestrial experiment can still to keep fresh state,
Illustrate the fruit freshness preserving film commonly using the present invention, phenomena such as can preventing fruit from producing weightless, shrinkage, maintain the outer of fruit
Sight and its quality of pulp, extend the pot-life of fruit.
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (7)
1. a kind of preparation method of the degradable antimicrobial preservative film of longan seed, it is characterised in that comprise the following steps：
(1) longan seed is cleaned and crushes, breaks into pulpous state, gained slurry is crossed into 100-120 mesh sieves, retain filter residue and filtrate respectively
It is standby；
(2) add 6-8 times of water of its quality into step (1) described filter residue and obtain filter residue mixed liquor, then into filter residue mixed liquor
Alpha-amylase is added, enzymolysis 2-3h is carried out under the conditions of 70-72 DEG C of temperature, the mixed liquor for digesting end is filtered and centrifuged,
Supernatant is collected, and sterilization treatment is carried out to supernatant；Cellulase is added into supernatant again, in 60-65 DEG C of thermostatted water
In bath, after digesting 1-2h, the enzyme deactivation 5-8min at a temperature of 80 DEG C -85 DEG C is taken out, filtrate is then collected after filtering, obtains enzyme
Solve product；
(3) step (2) described enzymolysis product is put into stainless steel extractor, 30- is extracted using subcritical extraction method
35min, collect filter liquor and obtain longan seed leaching liquor；
(4) step (1) the filtrate regulation pH value is placed in be ultrasonically treated in environment to 8-9 and handles 3-5min, stand 20-
Supernatant liquid is discharged after 30min；The pH value of regulation surplus materials is placed in be ultrasonically treated in environment for 8-9 again handles 3-5min,
Supernatant liquid is discharged after standing 20-30min；Dried at a temperature of remaining material is put in into 60-62 DEG C, obtain longan seed starch；
(5) pectin 1-2 parts are chosen according to parts by weight, soybean protein isolate 1-3 parts, PLA 20-40 parts, chitosan 1-8 parts,
Longan seed leaching liquor 2-6 parts, longan seed starch 8-15 parts, silica 0.02-0.08 parts, triethanolamine oleate 0.01-0.04
Part, potassium sorbate 0.1-0.6 parts, acetic acid 0.5-1.8 parts, horse propylene copolymer 1-5 parts, vegetable oil 2-8 parts, furfural 3-8 parts, fructose
4-10 parts, water 40-60 parts, plasticizer 1-3 parts, defoamer 0.5-1.5 parts, coupling agent 0.5-1.9 parts；Above-mentioned substance is mixed equal
It is even to be put into extruder, extrude preservative film under the conditions of being 140-145 DEG C in temperature.
2. the preparation method of the degradable antimicrobial preservative film of a kind of longan seed according to claim 1, it is characterised in that in step
Suddenly in (2), the usage amount of the alpha-amylase is the 0.05-0.08wt% of the filter residue mixed liquor.
3. the preparation method of the degradable antimicrobial preservative film of a kind of longan seed according to claim 2, it is characterised in that in step
Suddenly in (2), the usage amount of the cellulase is the 0.03-0.05wt% of the supernatant.
4. the preparation method of the degradable antimicrobial preservative film of a kind of longan seed according to claim 1, it is characterised in that in step
Suddenly in (3), the subcritical abstraction process is extracted under conditions of temperature is 105-115 DEG C, pressure is 2-3MPa.
5. the preparation method of the degradable antimicrobial preservative film of a kind of longan seed according to claim 1, it is characterised in that in step
Suddenly in (4), the power of the ultrasonic wave is 300-500W.
6. the preparation method of the degradable antimicrobial preservative film of a kind of longan seed according to claim 1, it is characterised in that in step
Suddenly in (5), according to parts by weight, 1.5 parts of pectin, 2 parts of soybean protein isolate, 30 parts of PLA, 6 parts of chitosan, longan seed are chosen
4 parts of leaching liquor, 10 parts of longan seed starch, 0.04 part of silica, 0.02 part of triethanolamine oleate, 0.5 part of potassium sorbate, acetic acid
0.9 part, it is 3 parts of horse propylene copolymer, 6 parts of vegetable oil, 4 parts of furfural, 6 parts of fructose, 50 parts of water, 2 parts of plasticizer, 0.8 part of defoamer, even
Join 1.2 parts of agent.
7. the preparation method of the degradable antimicrobial preservative film of a kind of longan seed according to claim 1, it is characterised in that in step
Suddenly in (5), the thickness of the preservative film is 8-10 μm.
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