CN107518017A - It is a kind of using plant tissue as crowndaisy chrysanthemum leaf blight pesticide control of raw material and preparation method thereof - Google Patents

It is a kind of using plant tissue as crowndaisy chrysanthemum leaf blight pesticide control of raw material and preparation method thereof Download PDF

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CN107518017A
CN107518017A CN201710953221.4A CN201710953221A CN107518017A CN 107518017 A CN107518017 A CN 107518017A CN 201710953221 A CN201710953221 A CN 201710953221A CN 107518017 A CN107518017 A CN 107518017A
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crowndaisy chrysanthemum
leaf blight
crowndaisy
water
weight
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/30—Polygonaceae [Buckwheat family], e.g. red-knees or rhubarb

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  • Agronomy & Crop Science (AREA)
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  • Natural Medicines & Medicinal Plants (AREA)
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Abstract

The present invention relates to a kind of crowndaisy chrysanthemum leaf blight pesticide control using plant tissue as raw material, medicament includes the raw material of following parts by weight：The fruit of ergcibe obtusifolia, the root of the petal , pivots storage of China pink, water.The root that the medicament of the preventing and treating crowndaisy chrysanthemum leaf blight is stored the petal, pivots of the fruit of fresh ergcibe obtusifolia, China pink by following steps obtained (1), grinds, and filtering, leaves juice, standby；(2) three kinds of water obtained above is concentrated under reduced pressure respectively, Extraction solvent is water, and obtained extract is dissolved with a small amount of water；(3) extract of three kinds of plant tissues is well mixed with water, it is standby；(4) mixing liquid is sprayed in crowndaisy chrysanthemum growth period.The present invention solves current crowndaisy chrysanthemum leaf blight present situation occurred frequently, while improves the content of potassium in crowndaisy chrysanthemum, is also stored these three plants using ergcibe obtusifolia, China pink, pivots to be more preferable and is provided new thinking.

Description

It is a kind of using plant tissue as crowndaisy chrysanthemum leaf blight pesticide control of raw material and preparation method thereof
It is on November 13rd, 2015 applying date that the application, which is, and Application No. 2015107786876 is entitled " a kind of For improving biological agent of content of potassium and preparation method thereof in crowndaisy chrysanthemum " patent divisional application.
Technical field
The present invention relates to a kind of biological agent, and in particular to a kind of crowndaisy chrysanthemum leaf blight using plant tissue as raw material prevents and treats medicine Agent and preparation method thereof, belong to pharmaceutical arts.
Background technology
Crowndaisy chrysanthemum, Classification system：Chrysanthemum coronarium L., crowndaisy chrysanthemum are composite family, Chrysanthemum, annual or two Year raw herbaceous plant, using young stem and leaf as the cultigen eaten, alias crowndaisy chrysanthemum, spring chrysanthemum, wormwood artemisia stalk.Crowndaisy chrysanthemum originates in Mediterranean, Europe People is usually used in doing parterre flower.Also there is the cultivation history of more than 1000 years in China, early in seventh century of Christian era the Tang Dynasty with regard on the books.Tong The appellation of wormwood artemisia is first appeared in the Tang Dynasty SUN Si miao《Essential Recipes for Emergent Use Worth A Thousand Gold》, the register of crowndaisy chrysanthemum has been shown in its vegetables class.In crowndaisy chrysanthemum The mineral salts such as sodium, potassium containing protein and higher amount, internal aqueous metabolism can be adjusted, eliminate oedema, current existing skill Crowndaisy chrysanthemum Minerals Quality Research is focused mostly in art in terms of extraction process, to being rarely reported in terms of content raising.Crowndaisy chrysanthemum leaf is withered Disease only infects blade, and scab is circular or irregular shape, and center is light grey, edge brown or light brown, during high humidity leaf back and Front has the conidium stigma and conidium of black mustiness thing, i.e. pathogen.The mutual joint of later stage scab is sick into bulk Spot, cause blade withered.Crowndaisy chrysanthemum is serious after catching an illness to reduce crowndaisy chrysanthemum yield and quality.Preventing and treating master of the prior art to crowndaisy chrysanthemum leaf blight It is to spray medicament, existing medicament has pollution to a certain extent to environment, and not efficient.
The content of the invention
It is an object of the invention to provide a kind of crowndaisy chrysanthemum leaf blight pesticide control using plant tissue as raw material, one kind is used to carry Biological agent of content of potassium and preparation method thereof in high crowndaisy chrysanthemum, the fertilizer safety non-pollution, manufacturing process is simple, and raw material is easy , the generation of crowndaisy chrysanthemum leaf blight can be effectively prevented and treated, while also add significantly the content of potassium in crowndaisy chrysanthemum.
To achieve the above object, the technical solution adopted by the present invention is：
A kind of crowndaisy chrysanthemum leaf blight pesticide control using plant tissue as raw material, it is characterised in that：The pesticide control is by following Method is made：
The first step, the root that the petal 45-55 parts by weight, pivots of the fruit 12-18 parts by weight of fresh ergcibe obtusifolia, China pink are stored 33-40 parts by weight, are fully ground in mortar respectively, wherein, preferred weight part of ergcibe obtusifolia fruit is 15 parts, the petal of China pink Preferred weight part is that the preferred weight part for the root that 50 Fen , pivots store is 40 parts；
Second step, three kinds of liquid that the first step is obtained are filtered respectively, are removed solid impurity, are left juice；
3rd step, three kinds of waters that second step is obtained are concentrated under reduced pressure respectively, then respectively with 50 parts by weight Water dissolves；
4th step, the plant tissue extract that the 3rd step obtains is well mixed with the water of 200 parts by weight, produces the preventing and treating The biological agent of crowndaisy chrysanthemum leaf blight.
Test site is that Jiangxi is newly regional, and the kind for testing crowndaisy chrysanthemum is great Ye crowndaisy chrysanthemums, is one kind of crowndaisy chrysanthemum, and August is just broadcast Kind, using bar broadcasting method, being emerged after 3-5 days, every plant of crowndaisy chrysanthemum sprayed the biological agent 15ml as made from above-mentioned steps every ten days, Every plant of crowndaisy chrysanthemum need to spray medicament 5 times altogether to before harvesting, and control group does not spray the medicament, only sprays water, and the crowndaisy chrysanthemum of every group of experiment shares 300 plants (every group of experiment is divided into three repetitions, i.e., often repeats 100 plants, test data is the average of three repetition the data obtaineds), Harvest within after planting 50 days, crowndaisy chrysanthemum of the observation with leaf blight simultaneously records strain number, and it is withered that those skilled in the art should know crowndaisy chrysanthemum leaf The criterion of disease, every group of experiment are chosen 150 plants of crowndaisy chrysanthemums (often repeating to randomly select 50 plants of crowndaisy chrysanthemums) using sampling survey method, carried Take and detect the content of the potassium in every plant of crowndaisy chrysanthemum blade, calculate the content and record of potassium in every gram of blade, obtain 150 numerical value, Average is calculated again.
The Ginding process that is related in the present invention, filter method, the method that is concentrated under reduced pressure, type of seeding, crowndaisy chrysanthemum field management The method of the content of potassium belongs to prior art in method, the potassium in extraction crowndaisy chrysanthemum and detection crowndaisy chrysanthemum.
The three kinds of plant materials used in the present invention, are now described below：
Ergcibe obtusifolia, Classification system：Erycibe obtusifolia, it is that Convolvulaceae ergcibe obtusifolia category climbs up by holding on to liana, berry ball Shape, the fruiting period 8-10 months, the raw material that the present invention uses are the fruits of ergcibe obtusifolia, are gathered when fruit length is to 2-3mm.
China pink, Classification system：Dianthus chinensis L., are Caryophyllaceae Carnation herbaceos perennials, plant height 30-40 centimetres, upright fasciation.Stem is upright, has section, multi-branched, leaf is to life, bar shaped or wire lanceolar.Petal sunny side middle and lower part Black beauty ring grain is formed, valve face such as dish dodges suede light when in full bloom, bright and colourful.In April at florescence to October, April is concentrated on to 5 Month, the raw material that the present invention uses is the petal of China pink, picks up from flower by the end of April.
Pivot stores, Classification system：Polygonum aviculare L., are polygonaceae Polygonum annual herb plants, florescence 6-8 Month, the fruiting period 9-10 months, the root that the raw material Shi pivots that the present invention uses store, pick up from by the end of August.
The present invention is a kind of crowndaisy chrysanthemum leaf blight pesticide control using plant tissue as raw material, it may also be said to it is a kind of dedicated for Prevent and treat biological agent and preparation method thereof of crowndaisy chrysanthemum leaf blight, or for improve in crowndaisy chrysanthemum the biological agent of potassium content and its Preparation method, while can also be for preventing and treating crowndaisy chrysanthemum leaf blight and improving the biological agent of potassium content in crowndaisy chrysanthemum, for crowndaisy chrysanthemum Plantation has good effect.
Embodiment
Embodiments of the invention and its result are further elaborated below, with the fruit of ergcibe obtusifolia, the petal of China pink, pivot The root of storage is three factors, and embodiment 1-9 design Three factors-levels orthogonal tests, embodiment 10-16 is pair of orthogonal test It is more identical than experiment, the parts by weight of all embodiment reclaimed waters.
Embodiment 1
A kind of crowndaisy chrysanthemum leaf blight pesticide control using plant tissue as raw material, it is characterised in that：The pesticide control is by following Method is made：
The first step, the weight of root 33 that the parts by weight, pivots of petal 45 of the parts by weight of fruit 12 of fresh ergcibe obtusifolia, China pink are stored Part, it is fully ground respectively in mortar, it is standby.The fruit diameter of the fresh ergcibe obtusifolia of harvesting is 2-3mm, is surveyed with spiral micrometer Amount, the petal of China pink is digging out in the root for plucking , pivots storage by the end of April and is removing soil by the end of August, when three of the above plant tissue is due to collection Between it is different, what is first gathered should be placed on Cord blood in moisture-keeping container, and store method belongs to what those skilled in the art knew.Separately Outside, spiral micrometer model M37570 used in experiment, purchased from the long-range Science and Technology Ltd. in Beijing Chinese and Western.
Second step, three kinds of liquid that the first step is obtained are filtered respectively, are removed solid impurity, are left juice, standby. Filter process belongs to prior art, carries out at room temperature.
3rd step, three kinds of waters that second step is obtained are respectively placed in round-bottomed flask, and instrument steams for rotation Instrument is sent out, Extraction solvent is water, repeats to extract repeatedly up to liquid evaporating completely in round-bottomed flask, then respectively with the water of 50 parts by weight Dissolving.The process of being concentrated under reduced pressure belongs to prior art.
4th step, the plant tissue extract that the 3rd step obtains is well mixed with the water of 200 parts by weight, produces the preventing and treating The biological agent of crowndaisy chrysanthemum leaf blight.
Test site and time：Newly regional for Jiangxi, August just starts to test.
Test explanation：1, the kind for testing crowndaisy chrysanthemum is great Ye crowndaisy chrysanthemums, is one kind of crowndaisy chrysanthemum, August is just sowed, using drilling side Method, emerged after 3-5 days.
2, every plant of crowndaisy chrysanthemum sprayed the biological agent 15ml as made from above-mentioned steps every ten days, and every plant of crowndaisy chrysanthemum is common to before harvesting Medicament need to be sprayed 5 times, the crowndaisy chrysanthemum of group experiment shares 300 plants, is divided into three repetitions, i.e., often repeats 100 plants.Note：Spray every time Medicament be all the identical medicament newly configured, the medicament sprayed every time, just do not used more than 24 hours, if run into it is rainy, Then identical medicament is sprayed again in 24 hours that rain stops, still, such supplement, which sprays not counted on, to be sprayed in number, and And also it is not qualified as the zero date sprayed next time.
3, total Test terminates by the end of September, while statistical results.
4, result of the test is：A, leaf blight strain number：Crowndaisy chrysanthemum of the observation with leaf blight simultaneously records strain number, and crowndaisy chrysanthemum leaf blight is only Infect blade, scab is circular or irregular shape, and center is light grey, edge brown or light brown, leaf back and front during high humidity Conidium stigma and conidium with black mustiness thing, i.e. pathogen.The mutual joint of later stage scab is led into bulk scab Cause blade withered.Those skilled in the art should know the criterion of crowndaisy chrysanthemum leaf blight.B, the content of potassium：Every group of experiment uses Sampling survey method chooses 150 plants of crowndaisy chrysanthemums (often repeating to randomly select 50 plants of crowndaisy chrysanthemums), extracts and detects in every plant of crowndaisy chrysanthemum blade The content of potassium, and record, 150 numerical value are obtained, then calculate average.The detection method of potassium content in sampling survey method, crowndaisy chrysanthemum And extracting method is prior art.
Embodiment 2
A kind of crowndaisy chrysanthemum leaf blight pesticide control using plant tissue as raw material, it is characterised in that：The pesticide control is by following Method is made：
The first step, the weight of root 38 that the parts by weight, pivots of petal 50 of the parts by weight of fruit 12 of fresh ergcibe obtusifolia, China pink are stored Part, it is fully ground respectively in mortar, it is standby.The fruit diameter of the fresh ergcibe obtusifolia of harvesting is 2-3mm, is surveyed with spiral micrometer Amount, the petal of China pink is digging out in the root for plucking , pivots storage by the end of April and is removing soil by the end of August, when three of the above plant tissue is due to collection Between it is different, what is first gathered should be placed on Cord blood in moisture-keeping container, and store method belongs to what those skilled in the art knew.Separately Outside, spiral micrometer model M37570 used in experiment, purchased from the long-range Science and Technology Ltd. in Beijing Chinese and Western.
Second step, three kinds of liquid that the first step is obtained are filtered respectively, are removed solid impurity, are left juice, standby. Filter process belongs to prior art, carries out at room temperature.
3rd step, three kinds of waters that second step is obtained are respectively placed in round-bottomed flask, and instrument steams for rotation Instrument is sent out, Extraction solvent is water, repeats to extract repeatedly up to liquid evaporating completely in round-bottomed flask, then respectively with the water of 50 parts by weight Dissolving.The process of being concentrated under reduced pressure belongs to prior art.
4th step, the plant tissue extract that the 3rd step obtains is well mixed with the water of 200 parts by weight, produces the preventing and treating The biological agent of crowndaisy chrysanthemum leaf blight.
Test site and time：Newly regional for Jiangxi, August just starts to test.
Test explanation：1, the kind for testing crowndaisy chrysanthemum is great Ye crowndaisy chrysanthemums, is one kind of crowndaisy chrysanthemum, August is just sowed, using drilling side Method, emerged after 3-5 days.
2, every plant of crowndaisy chrysanthemum sprayed the biological agent 15ml as made from above-mentioned steps every ten days, and every plant of crowndaisy chrysanthemum is common to before harvesting Medicament need to be sprayed 5 times, the crowndaisy chrysanthemum of group experiment shares 300 plants, is divided into three repetitions, i.e., often repeats 100 plants.Note：Spray every time Medicament be all the identical medicament newly configured, the medicament sprayed every time, just do not used more than 24 hours, if run into it is rainy, Then identical medicament is sprayed again in 24 hours that rain stops, still, such supplement, which sprays not counted on, to be sprayed in number, and And also it is not qualified as the zero date sprayed next time.
3, total Test terminates by the end of September, while statistical results.
4, result of the test is：A, leaf blight strain number：Crowndaisy chrysanthemum of the observation with leaf blight simultaneously records strain number, and crowndaisy chrysanthemum leaf blight is only Infect blade, scab is circular or irregular shape, and center is light grey, edge brown or light brown, leaf back and front during high humidity Conidium stigma and conidium with black mustiness thing, i.e. pathogen.The mutual joint of later stage scab is led into bulk scab Cause blade withered.Those skilled in the art should know the criterion of crowndaisy chrysanthemum leaf blight.B, the content of potassium：Every group of experiment uses Sampling survey method chooses 150 plants of crowndaisy chrysanthemums (often repeating to randomly select 50 plants of crowndaisy chrysanthemums), extracts and detects in every plant of crowndaisy chrysanthemum blade The content of potassium, and record, 150 numerical value are obtained, then calculate average.The detection method of potassium content in sampling survey method, crowndaisy chrysanthemum And extracting method is prior art.
Embodiment 3
A kind of crowndaisy chrysanthemum leaf blight pesticide control using plant tissue as raw material, it is characterised in that：The pesticide control is by following Method is made：
The first step, the weight of root 40 that the parts by weight, pivots of petal 55 of the parts by weight of fruit 12 of fresh ergcibe obtusifolia, China pink are stored Part, it is fully ground respectively in mortar, it is standby.The fruit diameter of the fresh ergcibe obtusifolia of harvesting is 2-3mm, is surveyed with spiral micrometer Amount, the petal of China pink is digging out in the root for plucking , pivots storage by the end of April and is removing soil by the end of August, when three of the above plant tissue is due to collection Between it is different, what is first gathered should be placed on Cord blood in moisture-keeping container, and store method belongs to what those skilled in the art knew.Separately Outside, spiral micrometer model M37570 used in experiment, purchased from the long-range Science and Technology Ltd. in Beijing Chinese and Western.
Second step, three kinds of liquid that the first step is obtained are filtered respectively, are removed solid impurity, are left juice, standby. Filter process belongs to prior art, carries out at room temperature.
3rd step, three kinds of waters that second step is obtained are respectively placed in round-bottomed flask, and instrument steams for rotation Instrument is sent out, Extraction solvent is water, repeats to extract repeatedly up to liquid evaporating completely in round-bottomed flask, then respectively with the water of 50 parts by weight Dissolving.The process of being concentrated under reduced pressure belongs to prior art.
4th step, the plant tissue extract that the 3rd step obtains is well mixed with the water of 200 parts by weight, produces the preventing and treating The biological agent of crowndaisy chrysanthemum leaf blight.
Test site and time：Newly regional for Jiangxi, August just starts to test.
Test explanation：1, the kind for testing crowndaisy chrysanthemum is great Ye crowndaisy chrysanthemums, is one kind of crowndaisy chrysanthemum, August is just sowed, using drilling side Method, emerged after 3-5 days.
2, every plant of crowndaisy chrysanthemum sprayed the biological agent 15ml as made from above-mentioned steps every ten days, and every plant of crowndaisy chrysanthemum is common to before harvesting Medicament need to be sprayed 5 times, the crowndaisy chrysanthemum of group experiment shares 300 plants, is divided into three repetitions, i.e., often repeats 100 plants.Note：Spray every time Medicament be all the identical medicament newly configured, the medicament sprayed every time, just do not used more than 24 hours, if run into it is rainy, Then identical medicament is sprayed again in 24 hours that rain stops, still, such supplement, which sprays not counted on, to be sprayed in number, and And also it is not qualified as the zero date sprayed next time.
3, total Test terminates by the end of September, while statistical results.
4, result of the test is：A, leaf blight strain number：Crowndaisy chrysanthemum of the observation with leaf blight simultaneously records strain number, and crowndaisy chrysanthemum leaf blight is only Infect blade, scab is circular or irregular shape, and center is light grey, edge brown or light brown, leaf back and front during high humidity Conidium stigma and conidium with black mustiness thing, i.e. pathogen.The mutual joint of later stage scab is led into bulk scab Cause blade withered.Those skilled in the art should know the criterion of crowndaisy chrysanthemum leaf blight.B, the content of potassium：Every group of experiment uses Sampling survey method chooses 150 plants of crowndaisy chrysanthemums (often repeating to randomly select 50 plants of crowndaisy chrysanthemums), extracts and detects in every plant of crowndaisy chrysanthemum blade The content of potassium, and record, 150 numerical value are obtained, then calculate average.The detection method of potassium content in sampling survey method, crowndaisy chrysanthemum And extracting method is prior art.
Embodiment 4
A kind of crowndaisy chrysanthemum leaf blight pesticide control using plant tissue as raw material, it is characterised in that：The pesticide control is by following Method is made：
The first step, the weight of root 38 that the parts by weight, pivots of petal 45 of the parts by weight of fruit 15 of fresh ergcibe obtusifolia, China pink are stored Part, it is fully ground respectively in mortar, it is standby.The fruit diameter of the fresh ergcibe obtusifolia of harvesting is 2-3mm, is surveyed with spiral micrometer Amount, the petal of China pink is digging out in the root for plucking , pivots storage by the end of April and is removing soil by the end of August, when three of the above plant tissue is due to collection Between it is different, what is first gathered should be placed on Cord blood in moisture-keeping container, and store method belongs to what those skilled in the art knew.Separately Outside, spiral micrometer model M37570 used in experiment, purchased from the long-range Science and Technology Ltd. in Beijing Chinese and Western.
Second step, three kinds of liquid that the first step is obtained are filtered respectively, are removed solid impurity, are left juice, standby. Filter process belongs to prior art, carries out at room temperature.
3rd step, three kinds of waters that second step is obtained are respectively placed in round-bottomed flask, and instrument steams for rotation Instrument is sent out, Extraction solvent is water, repeats to extract repeatedly up to liquid evaporating completely in round-bottomed flask, then respectively with the water of 50 parts by weight Dissolving.The process of being concentrated under reduced pressure belongs to prior art.
4th step, the plant tissue extract that the 3rd step obtains is well mixed with the water of 200 parts by weight, produces the preventing and treating The biological agent of crowndaisy chrysanthemum leaf blight.
Test site and time：Newly regional for Jiangxi, August just starts to test.
Test explanation：1, the kind for testing crowndaisy chrysanthemum is great Ye crowndaisy chrysanthemums, is one kind of crowndaisy chrysanthemum, August is just sowed, using drilling side Method, emerged after 3-5 days.
2, every plant of crowndaisy chrysanthemum sprayed the biological agent 15ml as made from above-mentioned steps every ten days, and every plant of crowndaisy chrysanthemum is common to before harvesting Medicament need to be sprayed 5 times, the crowndaisy chrysanthemum of group experiment shares 300 plants, is divided into three repetitions, i.e., often repeats 100 plants.Note：Spray every time Medicament be all the identical medicament newly configured, the medicament sprayed every time, just do not used more than 24 hours, if run into it is rainy, Then identical medicament is sprayed again in 24 hours that rain stops, still, such supplement, which sprays not counted on, to be sprayed in number, and And also it is not qualified as the zero date sprayed next time.
3, total Test terminates by the end of September, while statistical results.
4, result of the test is：A, leaf blight strain number：Crowndaisy chrysanthemum of the observation with leaf blight simultaneously records strain number, and crowndaisy chrysanthemum leaf blight is only Infect blade, scab is circular or irregular shape, and center is light grey, edge brown or light brown, leaf back and front during high humidity Conidium stigma and conidium with black mustiness thing, i.e. pathogen.The mutual joint of later stage scab is led into bulk scab Cause blade withered.Those skilled in the art should know the criterion of crowndaisy chrysanthemum leaf blight.B, the content of potassium：Every group of experiment uses Sampling survey method chooses 150 plants of crowndaisy chrysanthemums (often repeating to randomly select 50 plants of crowndaisy chrysanthemums), extracts and detects in every plant of crowndaisy chrysanthemum blade The content of potassium, and record, 150 numerical value are obtained, then calculate average.The detection method of potassium content in sampling survey method, crowndaisy chrysanthemum And extracting method is prior art.
Embodiment 5
A kind of crowndaisy chrysanthemum leaf blight pesticide control using plant tissue as raw material, it is characterised in that：The pesticide control is by following Method is made：
The first step, the weight of root 40 that the parts by weight, pivots of petal 50 of the parts by weight of fruit 15 of fresh ergcibe obtusifolia, China pink are stored Part, it is fully ground respectively in mortar, it is standby.The fruit diameter of the fresh ergcibe obtusifolia of harvesting is 2-3mm, is surveyed with spiral micrometer Amount, the petal of China pink is digging out in the root for plucking , pivots storage by the end of April and is removing soil by the end of August, when three of the above plant tissue is due to collection Between it is different, what is first gathered should be placed on Cord blood in moisture-keeping container, and store method belongs to what those skilled in the art knew.Separately Outside, spiral micrometer model M37570 used in experiment, purchased from the long-range Science and Technology Ltd. in Beijing Chinese and Western.
Second step, three kinds of liquid that the first step is obtained are filtered respectively, are removed solid impurity, are left juice, standby. Filter process belongs to prior art, carries out at room temperature.
3rd step, three kinds of waters that second step is obtained are respectively placed in round-bottomed flask, and instrument steams for rotation Instrument is sent out, Extraction solvent is water, repeats to extract repeatedly up to liquid evaporating completely in round-bottomed flask, then respectively with the water of 50 parts by weight Dissolving.The process of being concentrated under reduced pressure belongs to prior art.
4th step, the plant tissue extract that the 3rd step obtains is well mixed with the water of 200 parts by weight, produces the preventing and treating The biological agent of crowndaisy chrysanthemum leaf blight.
Test site and time：Newly regional for Jiangxi, August just starts to test.
Test explanation：1, the kind for testing crowndaisy chrysanthemum is great Ye crowndaisy chrysanthemums, is one kind of crowndaisy chrysanthemum, August is just sowed, using drilling side Method, emerged after 3-5 days.
2, every plant of crowndaisy chrysanthemum sprayed the biological agent 15ml as made from above-mentioned steps every ten days, and every plant of crowndaisy chrysanthemum is common to before harvesting Medicament need to be sprayed 5 times, the crowndaisy chrysanthemum of group experiment shares 300 plants, is divided into three repetitions, i.e., often repeats 100 plants.Note：Spray every time Medicament be all the identical medicament newly configured, the medicament sprayed every time, just do not used more than 24 hours, if run into it is rainy, Then identical medicament is sprayed again in 24 hours that rain stops, still, such supplement, which sprays not counted on, to be sprayed in number, and And also it is not qualified as the zero date sprayed next time.
3, total Test terminates by the end of September, while statistical results.
4, result of the test is：A, leaf blight strain number：Crowndaisy chrysanthemum of the observation with leaf blight simultaneously records strain number, and crowndaisy chrysanthemum leaf blight is only Infect blade, scab is circular or irregular shape, and center is light grey, edge brown or light brown, leaf back and front during high humidity Conidium stigma and conidium with black mustiness thing, i.e. pathogen.The mutual joint of later stage scab is led into bulk scab Cause blade withered.Those skilled in the art should know the criterion of crowndaisy chrysanthemum leaf blight.B, the content of potassium：Every group of experiment uses Sampling survey method chooses 150 plants of crowndaisy chrysanthemums (often repeating to randomly select 50 plants of crowndaisy chrysanthemums), extracts and detects in every plant of crowndaisy chrysanthemum blade The content of potassium, and record, 150 numerical value are obtained, then calculate average.The detection method of potassium content in sampling survey method, crowndaisy chrysanthemum And extracting method is prior art.
Embodiment 6
A kind of crowndaisy chrysanthemum leaf blight pesticide control using plant tissue as raw material, it is characterised in that：The pesticide control is by following Method is made：
The first step, the weight of root 33 that the parts by weight, pivots of petal 55 of the parts by weight of fruit 15 of fresh ergcibe obtusifolia, China pink are stored Part, it is fully ground respectively in mortar, it is standby.The fruit diameter of the fresh ergcibe obtusifolia of harvesting is 2-3mm, is surveyed with spiral micrometer Amount, the petal of China pink is digging out in the root for plucking , pivots storage by the end of April and is removing soil by the end of August, when three of the above plant tissue is due to collection Between it is different, what is first gathered should be placed on Cord blood in moisture-keeping container, and store method belongs to what those skilled in the art knew.Separately Outside, spiral micrometer model M37570 used in experiment, purchased from the long-range Science and Technology Ltd. in Beijing Chinese and Western.
Second step, three kinds of liquid that the first step is obtained are filtered respectively, are removed solid impurity, are left juice, standby. Filter process belongs to prior art, carries out at room temperature.
3rd step, three kinds of waters that second step is obtained are respectively placed in round-bottomed flask, and instrument steams for rotation Instrument is sent out, Extraction solvent is water, repeats to extract repeatedly up to liquid evaporating completely in round-bottomed flask, then respectively with the water of 50 parts by weight Dissolving.The process of being concentrated under reduced pressure belongs to prior art.
4th step, the plant tissue extract that the 3rd step obtains is well mixed with the water of 200 parts by weight, produces the preventing and treating The biological agent of crowndaisy chrysanthemum leaf blight.
Test site and time：Newly regional for Jiangxi, August just starts to test.
Test explanation：1, the kind for testing crowndaisy chrysanthemum is great Ye crowndaisy chrysanthemums, is one kind of crowndaisy chrysanthemum, August is just sowed, using drilling side Method, emerged after 3-5 days.
2, every plant of crowndaisy chrysanthemum sprayed the biological agent 15ml as made from above-mentioned steps every ten days, and every plant of crowndaisy chrysanthemum is common to before harvesting Medicament need to be sprayed 5 times, the crowndaisy chrysanthemum of group experiment shares 300 plants, is divided into three repetitions, i.e., often repeats 100 plants.Note：Spray every time Medicament be all the identical medicament newly configured, the medicament sprayed every time, just do not used more than 24 hours, if run into it is rainy, Then identical medicament is sprayed again in 24 hours that rain stops, still, such supplement, which sprays not counted on, to be sprayed in number, and And also it is not qualified as the zero date sprayed next time.
3, total Test terminates by the end of September, while statistical results.
4, result of the test is：A, leaf blight strain number：Crowndaisy chrysanthemum of the observation with leaf blight simultaneously records strain number, and crowndaisy chrysanthemum leaf blight is only Infect blade, scab is circular or irregular shape, and center is light grey, edge brown or light brown, leaf back and front during high humidity Conidium stigma and conidium with black mustiness thing, i.e. pathogen.The mutual joint of later stage scab is led into bulk scab Cause blade withered.Those skilled in the art should know the criterion of crowndaisy chrysanthemum leaf blight.B, the content of potassium：Every group of experiment uses Sampling survey method chooses 150 plants of crowndaisy chrysanthemums (often repeating to randomly select 50 plants of crowndaisy chrysanthemums), extracts and detects in every plant of crowndaisy chrysanthemum blade The content of potassium, and record, 150 numerical value are obtained, then calculate average.The detection method of potassium content in sampling survey method, crowndaisy chrysanthemum And extracting method is prior art.
Embodiment 7
A kind of crowndaisy chrysanthemum leaf blight pesticide control using plant tissue as raw material, it is characterised in that：The pesticide control is by following Method is made：
The first step, the weight of root 40 that the parts by weight, pivots of petal 45 of the parts by weight of fruit 18 of fresh ergcibe obtusifolia, China pink are stored Part, it is fully ground respectively in mortar, it is standby.The fruit diameter of the fresh ergcibe obtusifolia of harvesting is 2-3mm, is surveyed with spiral micrometer Amount, the petal of China pink is digging out in the root for plucking , pivots storage by the end of April and is removing soil by the end of August, when three of the above plant tissue is due to collection Between it is different, what is first gathered should be placed on Cord blood in moisture-keeping container, and store method belongs to what those skilled in the art knew.Separately Outside, spiral micrometer model M37570 used in experiment, purchased from the long-range Science and Technology Ltd. in Beijing Chinese and Western.
Second step, three kinds of liquid that the first step is obtained are filtered respectively, are removed solid impurity, are left juice, standby. Filter process belongs to prior art, carries out at room temperature.
3rd step, three kinds of waters that second step is obtained are respectively placed in round-bottomed flask, and instrument steams for rotation Instrument is sent out, Extraction solvent is water, repeats to extract repeatedly up to liquid evaporating completely in round-bottomed flask, then respectively with the water of 50 parts by weight Dissolving.The process of being concentrated under reduced pressure belongs to prior art.
4th step, the plant tissue extract that the 3rd step obtains is well mixed with the water of 200 parts by weight, produces the preventing and treating The biological agent of crowndaisy chrysanthemum leaf blight.
Test site and time：Newly regional for Jiangxi, August just starts to test.
Test explanation：1, the kind for testing crowndaisy chrysanthemum is great Ye crowndaisy chrysanthemums, is one kind of crowndaisy chrysanthemum, August is just sowed, using drilling side Method, emerged after 3-5 days.
2, every plant of crowndaisy chrysanthemum sprayed the biological agent 15ml as made from above-mentioned steps every ten days, and every plant of crowndaisy chrysanthemum is common to before harvesting Medicament need to be sprayed 5 times, the crowndaisy chrysanthemum of group experiment shares 300 plants, is divided into three repetitions, i.e., often repeats 100 plants.Note：Spray every time Medicament be all the identical medicament newly configured, the medicament sprayed every time, just do not used more than 24 hours, if run into it is rainy, Then identical medicament is sprayed again in 24 hours that rain stops, still, such supplement, which sprays not counted on, to be sprayed in number, and And also it is not qualified as the zero date sprayed next time.
3, total Test terminates by the end of September, while statistical results.
4, result of the test is：A, leaf blight strain number：Crowndaisy chrysanthemum of the observation with leaf blight simultaneously records strain number, and crowndaisy chrysanthemum leaf blight is only Infect blade, scab is circular or irregular shape, and center is light grey, edge brown or light brown, leaf back and front during high humidity Conidium stigma and conidium with black mustiness thing, i.e. pathogen.The mutual joint of later stage scab is led into bulk scab Cause blade withered.Those skilled in the art should know the criterion of crowndaisy chrysanthemum leaf blight.B, the content of potassium：Every group of experiment uses Sampling survey method chooses 150 plants of crowndaisy chrysanthemums (often repeating to randomly select 50 plants of crowndaisy chrysanthemums), extracts and detects in every plant of crowndaisy chrysanthemum blade The content of potassium, and record, 150 numerical value are obtained, then calculate average.The detection method of potassium content in sampling survey method, crowndaisy chrysanthemum And extracting method is prior art.
Embodiment 8
A kind of crowndaisy chrysanthemum leaf blight pesticide control using plant tissue as raw material, it is characterised in that：The pesticide control is by following Method is made：
The first step, the weight of root 33 that the parts by weight, pivots of petal 50 of the parts by weight of fruit 18 of fresh ergcibe obtusifolia, China pink are stored Part, it is fully ground respectively in mortar, it is standby.The fruit diameter of the fresh ergcibe obtusifolia of harvesting is 2-3mm, is surveyed with spiral micrometer Amount, the petal of China pink is digging out in the root for plucking , pivots storage by the end of April and is removing soil by the end of August, when three of the above plant tissue is due to collection Between it is different, what is first gathered should be placed on Cord blood in moisture-keeping container, and store method belongs to what those skilled in the art knew.Separately Outside, spiral micrometer model M37570 used in experiment, purchased from the long-range Science and Technology Ltd. in Beijing Chinese and Western.
Second step, three kinds of liquid that the first step is obtained are filtered respectively, are removed solid impurity, are left juice, standby. Filter process belongs to prior art, carries out at room temperature.
3rd step, three kinds of waters that second step is obtained are respectively placed in round-bottomed flask, and instrument steams for rotation Instrument is sent out, Extraction solvent is water, repeats to extract repeatedly up to liquid evaporating completely in round-bottomed flask, then respectively with the water of 50 parts by weight Dissolving.The process of being concentrated under reduced pressure belongs to prior art.
4th step, the plant tissue extract that the 3rd step obtains is well mixed with the water of 200 parts by weight, produces the preventing and treating The biological agent of crowndaisy chrysanthemum leaf blight.
Test site and time：Newly regional for Jiangxi, August just starts to test.
Test explanation：1, the kind for testing crowndaisy chrysanthemum is great Ye crowndaisy chrysanthemums, is one kind of crowndaisy chrysanthemum, August is just sowed, using drilling side Method, emerged after 3-5 days.
2, every plant of crowndaisy chrysanthemum sprayed the biological agent 15ml as made from above-mentioned steps every ten days, and every plant of crowndaisy chrysanthemum is common to before harvesting Medicament need to be sprayed 5 times, the crowndaisy chrysanthemum of group experiment shares 300 plants, is divided into three repetitions, i.e., often repeats 100 plants.Note：Spray every time Medicament be all the identical medicament newly configured, the medicament sprayed every time, just do not used more than 24 hours, if run into it is rainy, Then identical medicament is sprayed again in 24 hours that rain stops, still, such supplement, which sprays not counted on, to be sprayed in number, and And also it is not qualified as the zero date sprayed next time.
3, total Test terminates by the end of September, while statistical results.
4, result of the test is：A, leaf blight strain number：Crowndaisy chrysanthemum of the observation with leaf blight simultaneously records strain number, and crowndaisy chrysanthemum leaf blight is only Infect blade, scab is circular or irregular shape, and center is light grey, edge brown or light brown, leaf back and front during high humidity Conidium stigma and conidium with black mustiness thing, i.e. pathogen.The mutual joint of later stage scab is led into bulk scab Cause blade withered.Those skilled in the art should know the criterion of crowndaisy chrysanthemum leaf blight.B, the content of potassium：Every group of experiment uses Sampling survey method chooses 150 plants of crowndaisy chrysanthemums (often repeating to randomly select 50 plants of crowndaisy chrysanthemums), extracts and detects in every plant of crowndaisy chrysanthemum blade The content of potassium, and record, 150 numerical value are obtained, then calculate average.The detection method of potassium content in sampling survey method, crowndaisy chrysanthemum And extracting method is prior art.
Embodiment 9
A kind of crowndaisy chrysanthemum leaf blight pesticide control using plant tissue as raw material, it is characterised in that：The pesticide control is by following Method is made：
The first step, the weight of root 33 that the parts by weight, pivots of petal 55 of the parts by weight of fruit 18 of fresh ergcibe obtusifolia, China pink are stored Part, it is fully ground respectively in mortar, it is standby.The fruit diameter of the fresh ergcibe obtusifolia of harvesting is 2-3mm, is surveyed with spiral micrometer Amount, the petal of China pink is digging out in the root for plucking , pivots storage by the end of April and is removing soil by the end of August, when three of the above plant tissue is due to collection Between it is different, what is first gathered should be placed on Cord blood in moisture-keeping container, and store method belongs to what those skilled in the art knew.Separately Outside, spiral micrometer model M37570 used in experiment, purchased from the long-range Science and Technology Ltd. in Beijing Chinese and Western.
Second step, three kinds of liquid that the first step is obtained are filtered respectively, are removed solid impurity, are left juice, standby. Filter process belongs to prior art, carries out at room temperature.
3rd step, three kinds of waters that second step is obtained are respectively placed in round-bottomed flask, and instrument steams for rotation Instrument is sent out, Extraction solvent is water, repeats to extract repeatedly up to liquid evaporating completely in round-bottomed flask, then respectively with the water of 50 parts by weight Dissolving.The process of being concentrated under reduced pressure belongs to prior art.
4th step, the plant tissue extract that the 3rd step obtains is well mixed with the water of 200 parts by weight, produces the preventing and treating The biological agent of crowndaisy chrysanthemum leaf blight.
Test site and time：Newly regional for Jiangxi, August just starts to test.
Test explanation：1, the kind for testing crowndaisy chrysanthemum is great Ye crowndaisy chrysanthemums, is one kind of crowndaisy chrysanthemum, August is just sowed, using drilling side Method, emerged after 3-5 days.
2, every plant of crowndaisy chrysanthemum sprayed the biological agent 15ml as made from above-mentioned steps every ten days, and every plant of crowndaisy chrysanthemum is common to before harvesting Medicament need to be sprayed 5 times, the crowndaisy chrysanthemum of group experiment shares 300 plants, is divided into three repetitions, i.e., often repeats 100 plants.Note：Spray every time Medicament be all the identical medicament newly configured, the medicament sprayed every time, just do not used more than 24 hours, if run into it is rainy, Then identical medicament is sprayed again in 24 hours that rain stops, still, such supplement, which sprays not counted on, to be sprayed in number, and And also it is not qualified as the zero date sprayed next time.
3, total Test terminates by the end of September, while statistical results.
4, result of the test is：A, leaf blight strain number：Crowndaisy chrysanthemum of the observation with leaf blight simultaneously records strain number, and crowndaisy chrysanthemum leaf blight is only Infect blade, scab is circular or irregular shape, and center is light grey, edge brown or light brown, leaf back and front during high humidity Conidium stigma and conidium with black mustiness thing, i.e. pathogen.The mutual joint of later stage scab is led into bulk scab Cause blade withered.Those skilled in the art should know the criterion of crowndaisy chrysanthemum leaf blight.B, the content of potassium：Every group of experiment uses Sampling survey method chooses 150 plants of crowndaisy chrysanthemums (often repeating to randomly select 50 plants of crowndaisy chrysanthemums), extracts and detects in every plant of crowndaisy chrysanthemum blade The content of potassium, and record, 150 numerical value are obtained, then calculate average.The detection method of potassium content in sampling survey method, crowndaisy chrysanthemum And extracting method is prior art.
Orthogonal experiments given below.The root that the fruit of ergcibe obtusifolia, the petal, pivots of China pink store in form all refers to parts by weight Number.
Embodiment 10
A kind of crowndaisy chrysanthemum leaf blight pesticide control using plant tissue as raw material, it is characterised in that：The pesticide control is by following Method is made：
The first step, the parts by weight of fruit 15 of fresh ergcibe obtusifolia are fully ground in mortar, it is standby.The fresh fourth of harvesting The fruit diameter of public rattan is 2-3mm, is measured with spiral micrometer, and the material of collection should be placed on Cord blood in moisture-keeping container, is protected The method of depositing belongs to what those skilled in the art knew.In addition, spiral micrometer model M37570 used in experiment, purchased from north The long-range Science and Technology Ltd. in capital Chinese and Western.
Second step, the liquid that the first step is obtained are filtered, and are removed solid impurity, are left juice, standby.Filter process Belong to prior art, carry out at room temperature.
3rd step, the water that second step is obtained are placed in round-bottomed flask, and instrument is Rotary Evaporators, extraction Solvent is water, repeats to extract repeatedly up to liquid evaporating completely in round-bottomed flask, then is dissolved with the water of 50 parts by weight.The decompression is dense Compression process belongs to prior art.
4th step, the plant tissue extract that the 3rd step obtains is well mixed with the water of 200 parts by weight, produces the preventing and treating The biological agent of crowndaisy chrysanthemum leaf blight.
Test site and time：Newly regional for Jiangxi, August just starts to test.
Test explanation：1, the kind for testing crowndaisy chrysanthemum is great Ye crowndaisy chrysanthemums, is one kind of crowndaisy chrysanthemum, August is just sowed, using drilling side Method, emerged after 3-5 days.
2, every plant of crowndaisy chrysanthemum sprayed the biological agent 15ml as made from above-mentioned steps every ten days, and every plant of crowndaisy chrysanthemum is common to before harvesting Medicament need to be sprayed 5 times, the crowndaisy chrysanthemum of group experiment shares 300 plants, is divided into three repetitions, i.e., often repeats 100 plants.Note：Spray every time Medicament be all the identical medicament newly configured, the medicament sprayed every time, just do not used more than 24 hours, if run into it is rainy, Then identical medicament is sprayed again in 24 hours that rain stops, still, such supplement, which sprays not counted on, to be sprayed in number, and And also it is not qualified as the zero date sprayed next time.
3, total Test terminates by the end of September, while statistical results.
4, result of the test is：A, leaf blight strain number：Crowndaisy chrysanthemum of the observation with leaf blight simultaneously records strain number, and crowndaisy chrysanthemum leaf blight is only Infect blade, scab is circular or irregular shape, and center is light grey, edge brown or light brown, leaf back and front during high humidity Conidium stigma and conidium with black mustiness thing, i.e. pathogen.The mutual joint of later stage scab is led into bulk scab Cause blade withered.Those skilled in the art should know the criterion of crowndaisy chrysanthemum leaf blight.B, the content of potassium：Every group of experiment uses Sampling survey method chooses 150 plants of crowndaisy chrysanthemums (often repeating to randomly select 50 plants of crowndaisy chrysanthemums), extracts and detects in every plant of crowndaisy chrysanthemum blade The content of potassium, and record, 150 numerical value are obtained, then calculate average.The detection method of potassium content in sampling survey method, crowndaisy chrysanthemum And extracting method is prior art.
Embodiment 11
A kind of crowndaisy chrysanthemum leaf blight pesticide control using plant tissue as raw material, it is characterised in that：The pesticide control is by following Method is made：
The first step, the parts by weight of petal 50 of fresh China pink are fully ground in mortar, it is standby.The petal of China pink is 4 The end of month plucks, and the vegetable material of collection should be placed on Cord blood in moisture-keeping container, and store method belongs to those skilled in the art and known Dawn.
Second step, the liquid that the first step is obtained are filtered, and are removed solid impurity, are left juice, standby.Filter process Belong to prior art, carry out at room temperature.
3rd step, the water that second step is obtained are placed in round-bottomed flask, and instrument is Rotary Evaporators, extraction Solvent is water, repeats to extract repeatedly up to liquid evaporating completely in round-bottomed flask, then is dissolved with the water of 50 parts by weight.The decompression is dense Compression process belongs to prior art.
4th step, the plant tissue extract that the 3rd step obtains is well mixed with the water of 200 parts by weight, produces the preventing and treating The biological agent of crowndaisy chrysanthemum leaf blight.
Test site and time：Newly regional for Jiangxi, August just starts to test.
Test explanation：1, the kind for testing crowndaisy chrysanthemum is great Ye crowndaisy chrysanthemums, is one kind of crowndaisy chrysanthemum, August is just sowed, using drilling side Method, emerged after 3-5 days.
2, every plant of crowndaisy chrysanthemum sprayed the biological agent 15ml as made from above-mentioned steps every ten days, and every plant of crowndaisy chrysanthemum is common to before harvesting Medicament need to be sprayed 5 times, the crowndaisy chrysanthemum of group experiment shares 300 plants, is divided into three repetitions, i.e., often repeats 100 plants.Note：Spray every time Medicament be all the identical medicament newly configured, the medicament sprayed every time, just do not used more than 24 hours, if run into it is rainy, Then identical medicament is sprayed again in 24 hours that rain stops, still, such supplement, which sprays not counted on, to be sprayed in number, and And also it is not qualified as the zero date sprayed next time.
3, total Test terminates by the end of September, while statistical results.
4, result of the test is：A, leaf blight strain number：Crowndaisy chrysanthemum of the observation with leaf blight simultaneously records strain number, and crowndaisy chrysanthemum leaf blight is only Infect blade, scab is circular or irregular shape, and center is light grey, edge brown or light brown, leaf back and front during high humidity Conidium stigma and conidium with black mustiness thing, i.e. pathogen.The mutual joint of later stage scab is led into bulk scab Cause blade withered.Those skilled in the art should know the criterion of crowndaisy chrysanthemum leaf blight.B, the content of potassium：Every group of experiment uses Sampling survey method chooses 150 plants of crowndaisy chrysanthemums (often repeating to randomly select 50 plants of crowndaisy chrysanthemums), extracts and detects in every plant of crowndaisy chrysanthemum blade The content of potassium, and record, 150 numerical value are obtained, then calculate average.The detection method of potassium content in sampling survey method, crowndaisy chrysanthemum And extracting method is prior art.
Embodiment 12
A kind of crowndaisy chrysanthemum leaf blight pesticide control using plant tissue as raw material, it is characterised in that：The pesticide control is by following Method is made：
The first step, the parts by weight of root 40 that Xin Xian pivots store are fully ground in mortar, it is standby.The root that pivot stores is by the end of August Dig out and remove soil, be placed on Cord blood in moisture-keeping container, store method belongs to what those skilled in the art knew.
Second step, the liquid that the first step is obtained are filtered, and are removed solid impurity, are left juice, standby.Filter process Belong to prior art, carry out at room temperature.
3rd step, the water that second step is obtained are placed in round-bottomed flask, and instrument is Rotary Evaporators, extraction Solvent is water, repeats to extract repeatedly up to liquid evaporating completely in round-bottomed flask, then is dissolved with the water of 50 parts by weight.The decompression is dense Compression process belongs to prior art.
4th step, the plant tissue extract that the 3rd step obtains is well mixed with the water of 200 parts by weight, produces the preventing and treating The biological agent of crowndaisy chrysanthemum leaf blight.
Test site and time：Newly regional for Jiangxi, August just starts to test.
Test explanation：1, the kind for testing crowndaisy chrysanthemum is great Ye crowndaisy chrysanthemums, is one kind of crowndaisy chrysanthemum, August is just sowed, using drilling side Method, emerged after 3-5 days.
2, every plant of crowndaisy chrysanthemum sprayed the biological agent 15ml as made from above-mentioned steps every ten days, and every plant of crowndaisy chrysanthemum is common to before harvesting Medicament need to be sprayed 5 times, the crowndaisy chrysanthemum of group experiment shares 300 plants, is divided into three repetitions, i.e., often repeats 100 plants.Note：Spray every time Medicament be all the identical medicament newly configured, the medicament sprayed every time, just do not used more than 24 hours, if run into it is rainy, Then identical medicament is sprayed again in 24 hours that rain stops, still, such supplement, which sprays not counted on, to be sprayed in number, and And also it is not qualified as the zero date sprayed next time.
3, total Test terminates by the end of September, while statistical results.
4, result of the test is：A, leaf blight strain number：Crowndaisy chrysanthemum of the observation with leaf blight simultaneously records strain number, and crowndaisy chrysanthemum leaf blight is only Infect blade, scab is circular or irregular shape, and center is light grey, edge brown or light brown, leaf back and front during high humidity Conidium stigma and conidium with black mustiness thing, i.e. pathogen.The mutual joint of later stage scab is led into bulk scab Cause blade withered.Those skilled in the art should know the criterion of crowndaisy chrysanthemum leaf blight.B, the content of potassium：Every group of experiment uses Sampling survey method chooses 150 plants of crowndaisy chrysanthemums (often repeating to randomly select 50 plants of crowndaisy chrysanthemums), extracts and detects in every plant of crowndaisy chrysanthemum blade The content of potassium, and record, 150 numerical value are obtained, then calculate average.The detection method of potassium content in sampling survey method, crowndaisy chrysanthemum And extracting method is prior art.
Embodiment 13
A kind of crowndaisy chrysanthemum leaf blight pesticide control using plant tissue as raw material, it is characterised in that：The pesticide control is by following Method is made：
The first step, the parts by weight of fruit 15 of fresh ergcibe obtusifolia, the parts by weight of petal 50 of China pink are filled in mortar respectively Divide grinding, it is standby.The fruit diameter of the fresh ergcibe obtusifolia of harvesting is 2-3mm, is measured with spiral micrometer, the petal of China pink is 4 The end of month plucks, and for both the above plant tissue because acquisition time is different, what is first gathered should be placed on Cord blood in moisture-keeping container, protect The method of depositing belongs to what those skilled in the art knew.In addition, spiral micrometer model M37570 used in experiment, purchased from north The long-range Science and Technology Ltd. in capital Chinese and Western.
Second step, two kinds of liquid that the first step is obtained are filtered respectively, are removed solid impurity, are left juice, standby. Filter process belongs to prior art, carries out at room temperature.
3rd step, two kinds of waters that second step is obtained are respectively placed in round-bottomed flask, and instrument steams for rotation Instrument is sent out, Extraction solvent is water, repeats to extract repeatedly up to liquid evaporating completely in round-bottomed flask, then respectively with the water of 50 parts by weight Dissolving.The process of being concentrated under reduced pressure belongs to prior art.
4th step, the plant tissue extract that the 3rd step obtains is well mixed with the water of 200 parts by weight, produces the preventing and treating The biological agent of crowndaisy chrysanthemum leaf blight.
Test site and time：Newly regional for Jiangxi, August just starts to test.
Test explanation：1, the kind for testing crowndaisy chrysanthemum is great Ye crowndaisy chrysanthemums, is one kind of crowndaisy chrysanthemum, August is just sowed, using drilling side Method, emerged after 3-5 days.
2, every plant of crowndaisy chrysanthemum sprayed the biological agent 15ml as made from above-mentioned steps every ten days, and every plant of crowndaisy chrysanthemum is common to before harvesting Medicament need to be sprayed 5 times, the crowndaisy chrysanthemum of group experiment shares 300 plants, is divided into three repetitions, i.e., often repeats 100 plants.Note：Spray every time Medicament be all the identical medicament newly configured, the medicament sprayed every time, just do not used more than 24 hours, if run into it is rainy, Then identical medicament is sprayed again in 24 hours that rain stops, still, such supplement, which sprays not counted on, to be sprayed in number, and And also it is not qualified as the zero date sprayed next time.
3, total Test terminates by the end of September, while statistical results.
4, result of the test is：A, leaf blight strain number：Crowndaisy chrysanthemum of the observation with leaf blight simultaneously records strain number, and crowndaisy chrysanthemum leaf blight is only Infect blade, scab is circular or irregular shape, and center is light grey, edge brown or light brown, leaf back and front during high humidity Conidium stigma and conidium with black mustiness thing, i.e. pathogen.The mutual joint of later stage scab is led into bulk scab Cause blade withered.Those skilled in the art should know the criterion of crowndaisy chrysanthemum leaf blight.B, the content of potassium：Every group of experiment uses Sampling survey method chooses 150 plants of crowndaisy chrysanthemums (often repeating to randomly select 50 plants of crowndaisy chrysanthemums), extracts and detects in every plant of crowndaisy chrysanthemum blade The content of potassium, and record, 150 numerical value are obtained, then calculate average.The detection method of potassium content in sampling survey method, crowndaisy chrysanthemum And extracting method is prior art.
Embodiment 14
A kind of crowndaisy chrysanthemum leaf blight pesticide control using plant tissue as raw material, it is characterised in that：The pesticide control is by following Method is made：
The first step, the parts by weight of root 40 that the parts by weight, pivots of fruit 15 of fresh ergcibe obtusifolia are stored, respectively in mortar fully Grinding, it is standby.The fruit diameter of the fresh ergcibe obtusifolia of harvesting is 2-3mm, and the root of , pivots storage is measured by the end of August with spiral micrometer Digging out and remove soil, for both the above plant tissue because acquisition time is different, what is first gathered should be placed on Cord blood in moisture-keeping container, Store method belongs to what those skilled in the art knew.In addition, spiral micrometer model M37570 used in experiment, is purchased from The long-range Science and Technology Ltd. in Beijing Chinese and Western.
Second step, two kinds of liquid that the first step is obtained are filtered respectively, are removed solid impurity, are left juice, standby. Filter process belongs to prior art, carries out at room temperature.
3rd step, two kinds of waters that second step is obtained are respectively placed in round-bottomed flask, and instrument steams for rotation Instrument is sent out, Extraction solvent is water, repeats to extract repeatedly up to liquid evaporating completely in round-bottomed flask, then respectively with the water of 50 parts by weight Dissolving.The process of being concentrated under reduced pressure belongs to prior art.
4th step, the plant tissue extract that the 3rd step obtains is well mixed with the water of 200 parts by weight, produces the preventing and treating The biological agent of crowndaisy chrysanthemum leaf blight.
Test site and time：Newly regional for Jiangxi, August just starts to test.
Test explanation：1, the kind for testing crowndaisy chrysanthemum is great Ye crowndaisy chrysanthemums, is one kind of crowndaisy chrysanthemum, August is just sowed, using drilling side Method, emerged after 3-5 days.
2, every plant of crowndaisy chrysanthemum sprayed the biological agent 15ml as made from above-mentioned steps every ten days, and every plant of crowndaisy chrysanthemum is common to before harvesting Medicament need to be sprayed 5 times, the crowndaisy chrysanthemum of group experiment shares 300 plants, is divided into three repetitions, i.e., often repeats 100 plants.Note：Spray every time Medicament be all the identical medicament newly configured, the medicament sprayed every time, just do not used more than 24 hours, if run into it is rainy, Then identical medicament is sprayed again in 24 hours that rain stops, still, such supplement, which sprays not counted on, to be sprayed in number, and And also it is not qualified as the zero date sprayed next time.
3, total Test terminates by the end of September, while statistical results.
4, result of the test is：A, leaf blight strain number：Crowndaisy chrysanthemum of the observation with leaf blight simultaneously records strain number, and crowndaisy chrysanthemum leaf blight is only Infect blade, scab is circular or irregular shape, and center is light grey, edge brown or light brown, leaf back and front during high humidity Conidium stigma and conidium with black mustiness thing, i.e. pathogen.The mutual joint of later stage scab is led into bulk scab Cause blade withered.Those skilled in the art should know the criterion of crowndaisy chrysanthemum leaf blight.B, the content of potassium：Every group of experiment uses Sampling survey method chooses 150 plants of crowndaisy chrysanthemums (often repeating to randomly select 50 plants of crowndaisy chrysanthemums), extracts and detects in every plant of crowndaisy chrysanthemum blade The content of potassium, and record, 150 numerical value are obtained, then calculate average.The detection method of potassium content in sampling survey method, crowndaisy chrysanthemum And extracting method is prior art.
Embodiment 15
A kind of crowndaisy chrysanthemum leaf blight pesticide control using plant tissue as raw material, it is characterised in that：The pesticide control is by following Method is made：
The first step, the parts by weight of root 40 that the parts by weight, pivots of petal 50 of fresh China pink are stored, fully grinds in mortar respectively Mill, it is standby.The petal of China pink is digging out and is removing soil plucking the root that , pivots store by the end of April by the end of August, both the above plant tissue due to Acquisition time is different, and what is first gathered should be placed on Cord blood in moisture-keeping container, and store method belongs to those skilled in the art and known 's.
Second step, two kinds of liquid that the first step is obtained are filtered respectively, are removed solid impurity, are left juice, standby. Filter process belongs to prior art, carries out at room temperature.
3rd step, two kinds of waters that second step is obtained are respectively placed in round-bottomed flask, and instrument steams for rotation Instrument is sent out, Extraction solvent is water, repeats to extract repeatedly up to liquid evaporating completely in round-bottomed flask, then respectively with the water of 50 parts by weight Dissolving.The process of being concentrated under reduced pressure belongs to prior art.
4th step, the plant tissue extract that the 3rd step obtains is well mixed with the water of 200 parts by weight, produces the preventing and treating The biological agent of crowndaisy chrysanthemum leaf blight.
Test site and time：Newly regional for Jiangxi, August just starts to test.
Test explanation：1, the kind for testing crowndaisy chrysanthemum is great Ye crowndaisy chrysanthemums, is one kind of crowndaisy chrysanthemum, August is just sowed, using drilling side Method, emerged after 3-5 days.
2, every plant of crowndaisy chrysanthemum sprayed the biological agent 15ml as made from above-mentioned steps every ten days, and every plant of crowndaisy chrysanthemum is common to before harvesting Medicament need to be sprayed 5 times, the crowndaisy chrysanthemum of group experiment shares 300 plants, is divided into three repetitions, i.e., often repeats 100 plants.Note：Spray every time Medicament be all the identical medicament newly configured, the medicament sprayed every time, just do not used more than 24 hours, if run into it is rainy, Then identical medicament is sprayed again in 24 hours that rain stops, still, such supplement, which sprays not counted on, to be sprayed in number, and And also it is not qualified as the zero date sprayed next time.
3, total Test terminates by the end of September, while statistical results.
4, result of the test is：A, leaf blight strain number：Crowndaisy chrysanthemum of the observation with leaf blight simultaneously records strain number, and crowndaisy chrysanthemum leaf blight is only Infect blade, scab is circular or irregular shape, and center is light grey, edge brown or light brown, leaf back and front during high humidity Conidium stigma and conidium with black mustiness thing, i.e. pathogen.The mutual joint of later stage scab is led into bulk scab Cause blade withered.Those skilled in the art should know the criterion of crowndaisy chrysanthemum leaf blight.B, the content of potassium：Every group of experiment uses Sampling survey method chooses 150 plants of crowndaisy chrysanthemums (often repeating to randomly select 50 plants of crowndaisy chrysanthemums), extracts and detects in every plant of crowndaisy chrysanthemum blade The content of potassium, and record, 150 numerical value are obtained, then calculate average.The detection method of potassium content in sampling survey method, crowndaisy chrysanthemum And extracting method is prior art.
Embodiment 16
The embodiment does not spray medicament, only sprays water.
Test site and time：Newly regional for Jiangxi, August just starts to test.
Test explanation：1, the kind for testing crowndaisy chrysanthemum is great Ye crowndaisy chrysanthemums, is one kind of crowndaisy chrysanthemum, August is just sowed, using drilling side Method, emerged after 3-5 days.
2, every plant of crowndaisy chrysanthemum sprayed water 15ml every ten days, and every plant of crowndaisy chrysanthemum need to spray water 5 times altogether to before harvesting, group experiment Crowndaisy chrysanthemum shares 300 plants, is divided into three repetitions, i.e., often repeats 100 plants.
3, total Test terminates by the end of September, while statistical results.
4, result of the test is：A, leaf blight strain number：Crowndaisy chrysanthemum of the observation with leaf blight simultaneously records strain number, and crowndaisy chrysanthemum leaf blight is only Infect blade, scab is circular or irregular shape, and center is light grey, edge brown or light brown, leaf back and front during high humidity Conidium stigma and conidium with black mustiness thing, i.e. pathogen.The mutual joint of later stage scab is led into bulk scab Cause blade withered.Those skilled in the art should know the criterion of crowndaisy chrysanthemum leaf blight.B, the content of potassium：Every group of experiment uses Sampling survey method chooses 150 plants of crowndaisy chrysanthemums (often repeating to randomly select 50 plants of crowndaisy chrysanthemums), extracts and detects in every plant of crowndaisy chrysanthemum blade The content of potassium, and record, 150 numerical value are obtained, then calculate average.The detection method of potassium content in sampling survey method, crowndaisy chrysanthemum And extracting method is prior art.
Provide 10-embodiment of embodiment 16 this seven groups of comparative test results again below, wherein have this symbol of "-", Mean and do not add this kind of raw material.

Claims (2)

  1. A kind of 1. crowndaisy chrysanthemum leaf blight pesticide control using plant tissue as raw material, it is characterised in that be by following steps prepare and Into：
    The first step, the parts by weight of root 38 that the parts by weight, pivots of petal 50 of the parts by weight of fruit 12 of fresh ergcibe obtusifolia, China pink are stored, It is fully ground respectively in mortar, it is standby.The fruit diameter of the fresh ergcibe obtusifolia of harvesting is 2-3mm, is measured with spiral micrometer, The petal of China pink is digging out in the root for plucking , pivots storage by the end of April and is removing soil by the end of August；
    Second step, three kinds of liquid that the first step is obtained are filtered respectively, are removed solid impurity, are left juice, standby；
    3rd step, three kinds of waters that second step is obtained are respectively placed in round-bottomed flask, and instrument is Rotary Evaporators, Extraction solvent is water, and Extraction solvent is water, repeats to extract repeatedly up to liquid evaporating completely in round-bottomed flask, then respectively with 50 weights Measure the water dissolving of part；
    4th step, the plant tissue extract that the 3rd step obtains is well mixed with the water of about 200 parts by weight, produces the preventing and treating The biological agent of crowndaisy chrysanthemum leaf blight.
  2. 2. a kind of preparation method using plant tissue as the crowndaisy chrysanthemum leaf blight pesticide control of raw material, it is characterised in that including following Step：
    The first step, the parts by weight of root 38 that the parts by weight, pivots of petal 50 of the parts by weight of fruit 12 of fresh ergcibe obtusifolia, China pink are stored, It is fully ground respectively in mortar, it is standby.The fruit diameter of the fresh ergcibe obtusifolia of harvesting is 2-3mm, is measured with spiral micrometer, The petal of China pink is digging out in the root for plucking , pivots storage by the end of April and is removing soil by the end of August；
    Second step, three kinds of liquid that the first step is obtained are filtered respectively, are removed solid impurity, are left juice, standby；
    3rd step, three kinds of waters that second step is obtained are respectively placed in round-bottomed flask, and instrument is Rotary Evaporators, Extraction solvent is water, and Extraction solvent is water, repeats to extract repeatedly up to liquid evaporating completely in round-bottomed flask, then respectively with 50 weights Measure the water dissolving of part；
    4th step, the plant tissue extract that the 3rd step obtains is well mixed with the water of about 200 parts by weight, produces the preventing and treating The biological agent of crowndaisy chrysanthemum leaf blight.
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