CN107505698A - A kind of biological identification counting device and method - Google Patents
A kind of biological identification counting device and method Download PDFInfo
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- CN107505698A CN107505698A CN201710582376.1A CN201710582376A CN107505698A CN 107505698 A CN107505698 A CN 107505698A CN 201710582376 A CN201710582376 A CN 201710582376A CN 107505698 A CN107505698 A CN 107505698A
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- 238000000034 method Methods 0.000 title claims abstract description 30
- 238000012258 culturing Methods 0.000 claims description 7
- 239000012535 impurity Substances 0.000 claims description 4
- 238000013519 translation Methods 0.000 claims description 4
- 238000009825 accumulation Methods 0.000 claims description 3
- 230000005540 biological transmission Effects 0.000 claims description 3
- 238000004166 bioassay Methods 0.000 claims 1
- 239000002028 Biomass Substances 0.000 abstract description 8
- 208000001840 Dandruff Diseases 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000005611 electricity Effects 0.000 description 2
- 238000013467 fragmentation Methods 0.000 description 2
- 238000006062 fragmentation reaction Methods 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 238000011017 operating method Methods 0.000 description 2
- 238000000205 computational method Methods 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000009191 jumping Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 230000016776 visual perception Effects 0.000 description 1
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/24—Base structure
- G02B21/26—Stages; Adjusting means therefor
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/365—Control or image processing arrangements for digital or video microscopes
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
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Abstract
The invention discloses a kind of biological identification counting device, including control system, three dimensionality mobile device and microscope, image collecting device, image collecting device and control system electrical connection, three dimensionality mobile device and control system electrical connection are provided with microscopical eyepiece；Three dimensionality mobile device is provided with the plate that is connected, and culture dish is placed with the plate that is connected, and culture dish is located at below microscopical object lens.The present invention also discloses a kind of biological identification method of counting.Biomass on culture dish can accurately be calculated using biological identification counting device provided by the invention.Culture dish adjustment to microscope can clearly be shown to the position of biology by three dimensionality mobile device, then carry out subregion to culture dish by image collecting device to scan district by district, control system to the information of image acquisition device analyze district by district, recorded, statistics.
Description
Technical field
The present invention relates to a kind of counting device, more particularly to a kind of biological identification counting device and method.
Background technology
With gradually stepping up for quality of life, pursuit more and more higher of the people to quality of life, so having degerming, except mite
Household electrical appliance etc. function also arise at the historic moment.At present, existing in market largely can remove harmful organism (such as：Acarid)
Electrical equipment, such household electrical appliance are often marvelous in advertising, but because many harmful organism builds are small, often
Can not be by direct visual perception, so the service behaviour of such household electrical appliance also is difficult to verify.
The content of the invention
It is an object of the present invention to provide a kind of biological identification counting device, can accurately be calculated using the present apparatus
Biomass on culture dish, it can be used for examining the except mite effect with except mite functions domestic electrical equipment, the present invention also carries simultaneously
The application method of described device is supplied.
In order to solve the above technical problems, the present invention adopts the following technical scheme that：
A kind of biological identification counting device, including control system, three dimensionality mobile device and microscope, microscopical mesh
Jing Chu is provided with image collecting device, image collecting device and control system electrical connection, three dimensionality mobile device and control system electricity
Connection；Three dimensionality mobile device is provided with the plate that is connected, and is placed with culture dish on the plate that is connected, culture dish is located at microscopical object lens
Lower section.Three dimensionality mobile device can drive connected plate to be moved along three dimensions, on the one hand make culture dish positioned at correct
Position single individual biological above culture dish can be shown by microscope；On the other hand, image acquisition device
On the culture dish of current state after biological situation, three dimensionality mobile device can translate, and image collecting device is passed through aobvious
The biological information of other states on micro mirror collection culture dish.
In a kind of foregoing biological identification counting device, the three dimensionality mobile device includes X-axis moving track, Y-axis
Moving track, Z axis mobile device, the first translating device and the second translating device, the first translating device are arranged on X-axis moving rail
On road, Y-axis moving track is arranged on the first translating device, and the second translating device is arranged on Y-axis moving track, Z axis movement
Device is arranged on the second translating device, and the connected plate is arranged in Z axis mobile device；Z axis mobile device, the first translation
Device and the second translating device electrically connect with control system.
As one of which embodiment, the connected plate is provided with load-carrying ring, and culture dish is located on load-carrying ring.Pass through
During micro- sem observation culture dish, being able to observe that in culture dish has clearly biomass information.
, can be more using this method present invention also offers a kind of foregoing application method of biological identification counting device
It is accurate to calculate biomass on culture dish.A kind of biological identification method of counting, using above-described biological identification
Counting device, including following methods：To have zoic culture dish first to be placed in three dimensionality mobile device, pass through control
First translating device and the second translating device make culture dish be located at below microscopical object lens；Then by adjusting Z axis movement dress
Put and microscope, microscope is shown single individual biological on culture dish；Image collecting device will be adopted by microscope
Biological information transmission is to control system on the culture dish collected, the image letter that control system scan image harvester is sent
Breath, is counted to the biology on culture dish.
In a kind of foregoing biological identification method of counting, in addition to following methods：Image collecting device is every time by aobvious
The biometric image information of designated area on micro mirror collection culture dish, image collecting device are successively clapped designated area through microscope
According to twice, take pictures at intervals of 0.2 to 10 second；Result of taking pictures twice is compared, if biology does not move, is considered as dead
Die biology.The single that the designated area refers to specifying counts area, every time to the culture dish biomass in unit area
Counted, then added up successively, completed until biology all in culture dish is added up.
In a kind of foregoing biological identification method of counting, in addition to following methods：Demarcate the size of living body biological, size
It is considered as impurity for less than 30% object that living body biological is dimensioned, not in accumulation range；Size is demarcated for living body biological
The object of the 30% to 65% of size is denoted as 0.5 dead organisms, and size is 65% to 100% that living body biological is dimensioned
Object be denoted as 1 dead organisms.Dead organisms are because damaged or fracture occurs for the reason such as experiment, it is also possible to multiple adhesions
Together, therefore one distinguishing rule substantially of formulation helps to calculate the quantity of dead organisms.
In a kind of foregoing biological identification method of counting, if recognizing the demarcation chi that object is more than the living body biological
Multiple biologies that are very little, then regarding the object to be sticked together, according to being dimensioned for the size of the object and the living body biological
The number that the object includes biology is calculated, computational methods are to do the size of the object and being dimensioned for the living body biological
Than ratio is the number that the object includes biology.
In a kind of foregoing biological identification method of counting, complete after once counting, 90 ° to 180 ° of rotating and culturing ware, enter
Second of counting of row；Complete second after counting, then 90 ° to 180 ° of rotating and culturing ware in same direction, carry out third time meter
Number；If count results three times, biology total deviation is less than 5%, and the average value for taking count results three times is effective result.
Compared with prior art, culture dish can accurately be counted using biological identification counting device provided by the invention
Upper biomass.Culture dish adjustment to microscope can clearly be shown to the position of biomass by three dimensionality mobile device
Put, then culture dish is taken pictures by image collecting device, carry out subregion by control system scans district by district, controls
System to the information of image acquisition device analyze district by district, recorded, statistics.So using the present apparatus and the present invention
The method of offer can accurately calculate the biomass of designated area on culture dish.
Brief description of the drawings
Fig. 1 is a kind of structural representation of embodiment of the present invention；
Fig. 2 is a kind of structural representation of embodiment of three dimensionality mobile device.
Reference：1- control systems, 2-X axle moving tracks, the translating devices of 3- first, 4-Y axle moving tracks, 5-Z axles
Mobile device, the translating devices of 6- second, 7- image collecting devices, 8- microscopes, 9- three dimensionality mobile devices, 10- load-carrying rings.
The present invention is further illustrated with reference to the accompanying drawings and detailed description.
Embodiment
Embodiments of the invention 1：As depicted in figs. 1 and 2, a kind of biological identification counting device, including control system 1,
Three dimensionality mobile device 9 and microscope 8, image collecting device 7, image collecting device 7 and control are provided with the eyepiece of microscope 8
System 1 processed electrically connects, and three dimensionality mobile device 9 and control system 1 electrically connect；Three dimensionality mobile device 9 is provided with the plate that is connected,
Culture dish 11 is placed with connected plate, culture dish 11 is located at below the object lens of microscope 8.
Specifically, the three dimensionality mobile device 9 include X-axis moving track 2, Y-axis moving track 4, Z axis mobile device 5,
First translating device 3 and the second translating device 6, the first translating device 3 are arranged on X-axis moving track 2, Y-axis moving track 4
It is arranged on the first translating device 3, the second translating device 6 is arranged on Y-axis moving track 4, and Z axis mobile device 5 is arranged on
On two translating devices 6, the connected plate is arranged in Z axis mobile device 5；Z axis mobile device 5, the first translating device 3 and
Two translating devices 6 electrically connect with control system 1.The connected plate is provided with load-carrying ring 10, and culture dish is located at load-carrying ring 10
On.
The operating method of embodiment 1：The identification method of counting of a kind of acarid, using the identification of the acarid described in embodiment 1
Counting device, including following methods：The culture dish 11 with acarid is placed on load-carrying ring 10 first.Pass through control first
The translating device 6 of translating device 3 and second makes culture dish 11 be located at below the object lens of microscope 8；Then moved by adjusting Z axis
Device 5 and microscope 8, microscope 8 is set to show the single individual of acarid on culture dish 11；Image collecting device 7 will pass through
The information transmission of acarid is to control system 1, the scan image harvester of control system 1 on the culture dish 11 that microscope 8 collects
7 image informations sent, are counted to the acarid on culture dish 11, and culture dish 11 is driven by three dimensionality mobile device 9,
Microscope 8 is shown the acarid in culture dish 11 line by line along own zig-zag path, and counted.In the present embodiment, control
System 1 processed is computer, and the identification and counting to acarid on image can call matlab algorithms real by image recognition program
It is existing.
Image collecting device 7 gathers the acarid image information on culture dish 11 in designated area, figure by microscope 8 every time
As harvester 7 through microscope 8 to designated area successively take pictures twice, take pictures at intervals of 0.2 second；To result of taking pictures twice
It is compared, if mobile or feeler is not swung acarid, is considered as dead acarid.The size of live body acarid is demarcated, if identification
It is more than being dimensioned for the live body acarid to object, and the object feeler quantity is more than single acarid feeler quantity, then regards
The object is the multiple acarids being sticked together, should according to the calculating that is dimensioned of the size of the object and the live body acarid
Object includes the number of acarid；Dust mite mite body is in Long Circle, long (260~400) μm, wherein, male mite body grows (260-
360) μm, female mite body grows (360-400) μm, needs to consider male mite and female mite when the size of live body acarid is demarcated
Individual difference, to set more rational live body acarid size.Because the acarid of death is likely to occur fragmentation and adhesion, text
Described in situation be fragmentation situation, may additionally stick together, exactly several acarids stick together and can not separated, and pass through
The estimation of area, it artificial is counted as several acarids.
Complete after once counting, (11) 90 ° of rotating and culturing ware, carry out second and count；Complete second after counting, then edge
(11) 180 ° of equidirectional rotating and culturing ware, carry out third time counting；If count results three times, biology total deviation is small
In 5%, the average value for taking count results three times is effective result.
Embodiment 2：As shown in Figure 1 and Figure 2, a kind of biological identification counting device, including control system 1, three dimensionality move
Device 9 and microscope 8 are moved, image collecting device 7, image collecting device 7 and the electricity of control system 1 are provided with the eyepiece of microscope 8
Connection, three dimensionality mobile device 9 and control system 1 electrically connect；Three dimensionality mobile device 9 is provided with the plate that is connected, and is put on the plate that is connected
Culture dish 11 is equipped with, culture dish 11 is located at below the object lens of microscope 8.
Specifically, the three dimensionality mobile device 9 include X-axis moving track 2, Y-axis moving track 4, Z axis mobile device 5,
First translating device 3 and the second translating device 6, the first translating device 3 are arranged on X-axis moving track 2, Y-axis moving track 4
It is arranged on the first translating device 3, the second translating device 6 is arranged on Y-axis moving track 4, and Z axis mobile device 5 is arranged on
On two translating devices 6, the connected plate is arranged in Z axis mobile device 5；Z axis mobile device 5, the first translating device 3 and
Two translating devices 6 electrically connect with control system 1；The connected plate is provided with load-carrying ring 10, and load-carrying ring 10, which is provided with, has training
Ware 11 is supported, is disturbed in culture dish 11 with jump.
The operating method of embodiment 2：A kind of biological identification method of counting, using the biological identification described in embodiment 2
Counting device, including following methods：The culture dish 11 disturbed with jump is placed on load-carrying ring 10 first, passes through control first
The translating device 6 of translating device 3 and second makes culture dish 11 be located at below the object lens of microscope 8；Then moved by adjusting Z axis
Device 5 and microscope 8, enable microscope 8 to show the single individual jumped and disturbed on culture dish 11, and the focusing of microscope 8 can lead to
Regulation manually is crossed, can also be realized by automatically adjusting；Image collecting device 7 believes the image collected by microscope 8
Breath passes to control system 1, the image information that the scan image harvester 7 of control system 1 is sent, to culture dish in image 11
On jump disturb and counted.
Image collecting device 7 gathers the image information on culture dish 11 in designated area by microscope 8 every time, and image is adopted
Acquisition means 7 are successively taken pictures twice through microscope 8 to designated area, are taken pictures at intervals of 10 seconds；Result of taking pictures twice is compared
It is right, do not moved if jumping to disturb, be considered as dead jump and disturb.Size is disturbed less than 30% object being dimensioned for live body jump and is considered as
Impurity, not in accumulation range；Size disturbs 30% to 65% object being dimensioned for live body jump and is denoted as 0.5 dead jump
Disturb, size is that live body is jumped to disturb 65% to 100% object that is dimensioned and be denoted as 1 dead jumps and disturbed.Control system 1 is right successively
Live body jump in adjacent designated area is disturbed, dead jump is disturbed and jump disturbs sum and is scanned, counts, and is added up.
Material in culture dish 11 can be generally divided into 3 kinds, be respectively：Scurf, it is single it is living jump disturb or extremely jump disturb, adhesion
Dead jump is disturbed；It is ordered as according to size：Scurf<Single jump is disturbed<Adhesion is extremely jumped and disturbed, so determining that face is disturbed in minimum and maximum jump
Product is critically important, and the material that area is disturbed less than most jete must be scurf or other impurities, and jump living more than maximum disturbs area
Material must be that adhesion extremely jump is disturbed.The single dead jump of adhesion disturbs average area and means that area is disturbed in adhesion extremely jump divided by dead jump is disturbed
Number, disturb average area accurately to single dead jump of adhesion, n heaps can also be taken, the adhesion of n heaps, which is extremely jumped, disturbs area addition
Divided by the number disturbed extremely is jumped in n heaps.
Complete after once counting, 180 ° of rotating and culturing ware, carry out second and count；Complete second count after, then along phase
Equidirectional 90 ° of rotating and culturing ware, carry out third time counting；If count results three times, biology total deviation is less than 5%, takes
The average value of count results is effective result three times.
Claims (9)
1. a kind of biological identification counting device, it is characterised in that including control system (1), three dimensionality mobile device (9) and show
Micro mirror (8), image collecting device (7) is provided with the eyepiece of microscope (8), image collecting device (7) and control system (1) are electrically connected
Connect, three dimensionality mobile device (9) and control system (1) electrical connection；Three dimensionality mobile device (9) is provided with the plate that is connected, and be connected plate
On be placed with culture dish, culture dish is located at below the object lens of microscope (8).
A kind of 2. biological identification counting device according to claim 1, it is characterised in that the three dimensionality mobile device
(9) X-axis moving track (2), Y-axis moving track (4), Z axis mobile device (5), the first translating device (3) and the second translation are included
Device (6), the first translating device (3) are arranged on X-axis moving track (2), and Y-axis moving track (4) is arranged on the first translation dress
Put on (3), the second translating device (6) is arranged on Y-axis moving track (4), and Z axis mobile device (5) is arranged on the second translation dress
Put on (6), the connected plate is arranged in Z axis mobile device (5)；Z axis mobile device (5), the first translating device (3) and second
Translating device (6) electrically connects with control system (1).
3. a kind of biological identification counting device according to claim 2, it is characterised in that the connected plate is provided with and held
Ring (10) is carried, culture dish is located on load-carrying ring (10).
4. a kind of biological identification method of counting, it is characterised in that counted using the biological identification described in Claims 2 or 3
Device, including following methods：To have zoic culture dish first to be placed in three dimensionality mobile device (9), pass through control the
One translating device (3) and the second translating device (6) make culture dish be located at below the object lens of microscope (8)；Then by adjusting Z axis
Mobile device (5) and microscope (8), make microscope (8) to show single individual biological on culture dish；Image collecting device
(7) control system (1), control system (1) scanning will be given by information transmission biological on culture dish that microscope (8) collect
The image information that image collecting device (7) is sent, is counted to the biology on culture dish.
5. a kind of biological identification method of counting according to claim 4, it is characterised in that also including following methods：Figure
As harvester (7) gathers the biometric image information of designated area on culture dish, image collecting device by microscope (8) every time
(7) designated area is successively taken pictures twice through microscope (8), taken pictures at intervals of 0.2 to 10 second；To result of taking pictures twice
It is compared, if biology does not move, is considered as dead organisms.
6. a kind of biological identification method of counting according to claim 5, it is characterised in that also including following methods：Mark
Determine the size of living body biological, culture dish inside dimension is that less than 30% object that living body biological is dimensioned is considered as impurity, is not existed
In accumulation range；Size is that 30% to 65% object that living body biological is dimensioned is denoted as 0.5 dead organisms, and size is work
65% to 100% object of body biological standardization size is denoted as 1 dead organisms.
7. a kind of biological identification method of counting according to claim 5, it is characterised in that also including following methods：Control
System (1) processed is scanned to the living body biological in culture dish, dead organisms and biology total, counted, and adds up.
8. a kind of biological identification method of counting according to claim 7, it is characterised in that be more than if recognizing object
The living body biological is dimensioned, then the multiple biologies for regarding the object to be sticked together, according to the size of the object and institute
State being dimensioned for living body biological and calculate the number that the object includes biology.
9. a kind of biological identification method of counting according to claim 8, it is characterised in that complete after once counting, rotation
Turn 90 ° to 180 ° of culture dish, carry out second and count；Complete second count after, then 90 ° of rotating and culturing ware is extremely in same direction
180 °, carry out third time counting；If count results three times, biology total deviation is less than 5%, takes the flat of count results three times
Average is effective result.
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| CN204065550U (en) * | 2014-07-29 | 2014-12-31 | 杭州卓腾信息技术有限公司 | Based on microscopical digital slices automatic sweep apparatus |
| CN204462530U (en) * | 2015-01-19 | 2015-07-08 | 苏州迈迪达医疗器械科技有限公司 | A kind of copra analysis instrument micrometron device |
| CN105303180A (en) * | 2015-10-28 | 2016-02-03 | 清华大学 | Caenorhabditis elegans capture system and caenorhabditis elegans capture method |
| CN105403989A (en) * | 2015-10-28 | 2016-03-16 | 清华大学 | Nematode recognition system and nematode recognition method |
| CN105652429A (en) * | 2016-03-22 | 2016-06-08 | 哈尔滨理工大学 | Automatic focusing method for microscope cell glass slide scanning based on machine learning |
| CN106873142A (en) * | 2017-03-15 | 2017-06-20 | 北方工业大学 | High-quality image acquisition device and method of tubercle bacillus detector |
| CN207301477U (en) * | 2017-07-17 | 2018-05-01 | 中国家用电器研究院 | A kind of biological identification counting device |
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