CN107496435A - Application of the anoectochilus roxburghii glycosides in anti-fatigue medicament is prepared - Google Patents
Application of the anoectochilus roxburghii glycosides in anti-fatigue medicament is prepared Download PDFInfo
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- CN107496435A CN107496435A CN201710712122.7A CN201710712122A CN107496435A CN 107496435 A CN107496435 A CN 107496435A CN 201710712122 A CN201710712122 A CN 201710712122A CN 107496435 A CN107496435 A CN 107496435A
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- food
- anoectochilus
- fatigue
- anoectochilus roxburghii
- fatigue medicament
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- 241000934230 Anoectochilus roxburghii Species 0.000 title claims abstract description 48
- 150000002338 glycosides Chemical class 0.000 title claims abstract description 46
- 229930182470 glycoside Natural products 0.000 title claims abstract description 44
- 230000002929 anti-fatigue Effects 0.000 title claims abstract description 35
- 239000003814 drug Substances 0.000 title claims abstract description 35
- 235000013305 food Nutrition 0.000 claims abstract description 18
- 241000719837 Anoectochilus Species 0.000 claims description 16
- 241000632227 Antenoron Species 0.000 claims description 16
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 11
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 9
- 238000010828 elution Methods 0.000 claims description 8
- 210000004369 blood Anatomy 0.000 claims description 7
- 239000008280 blood Substances 0.000 claims description 7
- 239000000284 extract Substances 0.000 claims description 7
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- 239000000287 crude extract Substances 0.000 claims description 6
- 229940079593 drug Drugs 0.000 claims description 6
- 241000196324 Embryophyta Species 0.000 claims description 5
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 5
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- 229910052737 gold Inorganic materials 0.000 claims description 5
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- 239000008619 Xingren Substances 0.000 claims description 3
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- WGLUMOCWFMKWIL-UHFFFAOYSA-N dichloromethane;methanol Chemical compound OC.ClCCl WGLUMOCWFMKWIL-UHFFFAOYSA-N 0.000 claims description 3
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- 241000699670 Mus sp. Species 0.000 description 13
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- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 8
- MQEPWBMWFIVRPS-ZGSHZZHUSA-N (3R)-5-Oxotetrahydro-3-furanyl beta-D-glucopyranoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1CC(=O)OC1 MQEPWBMWFIVRPS-ZGSHZZHUSA-N 0.000 description 6
- MQEPWBMWFIVRPS-UHFFFAOYSA-N goodyeroside A Natural products OC1C(O)C(O)C(CO)OC1OC1CC(=O)OC1 MQEPWBMWFIVRPS-UHFFFAOYSA-N 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 5
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- 101710088194 Dehydrogenase Proteins 0.000 description 4
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- 235000014655 lactic acid Nutrition 0.000 description 4
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- VZGDMQKNWNREIO-UHFFFAOYSA-N tetrachloromethane Chemical compound ClC(Cl)(Cl)Cl VZGDMQKNWNREIO-UHFFFAOYSA-N 0.000 description 2
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- 102000003855 L-lactate dehydrogenase Human genes 0.000 description 1
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- ZSJLQEPLLKMAKR-UHFFFAOYSA-N Streptozotocin Natural products O=NN(C)C(=O)NC1C(O)OC(CO)C(O)C1O ZSJLQEPLLKMAKR-UHFFFAOYSA-N 0.000 description 1
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- 230000002218 hypoglycaemic effect Effects 0.000 description 1
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- -1 lactone glycoside Chemical class 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
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- ZSJLQEPLLKMAKR-GKHCUFPYSA-N streptozocin Chemical compound O=NN(C)C(=O)N[C@H]1[C@@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O ZSJLQEPLLKMAKR-GKHCUFPYSA-N 0.000 description 1
- 229960001052 streptozocin Drugs 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
- A61K31/7048—Compounds having saccharide radicals and heterocyclic rings having oxygen as a ring hetero atom, e.g. leucoglucosan, hesperidin, erythromycin, nystatin, digitoxin or digoxin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- General Health & Medical Sciences (AREA)
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- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Molecular Biology (AREA)
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- Polymers & Plastics (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to pharmaceutical technology field, specifically application of the anoectochilus roxburghii glycosides in anti-fatigue medicament or food is prepared.The invention provides the new medical usage of anoectochilus roxburghii glycosides, show through pharmacodynamic study, anoectochilus roxburghii glycosides of the invention can significantly extend heavy burden mouse swimming time, antifatigue significant effect, available for preparing anti-fatigue medicament or food.The present invention provides a kind of new source to seek new anti-fatigue medicament.
Description
Technical field
The present invention relates to pharmaceutical technology field, is to be prepared using Anoectochilus Blume and its relative genus plant as raw material specifically
Application of the anoectochilus roxburghii glycosides in anti-fatigue medicament or food.
Background technology
Fatigue is a kind of Physiological Psychology phenomenon, is that body is mental excessive with physical exertion, caused by the factor such as direct stimulation.One
As can be divided into periphery fatigue, the class of central nerve fatigue two.With expanding economy, the quickening of rhythm of life, prolonged work and
It cannot get effective sleep or life be irregular, cause increasing people to feel exhausted, myalgia, absent minded, note
Recall power decline, depressed, sleep-disorder etc..These symptoms, clinically general diagnostic is fatigue, covers periphery and maincenter is tired
Labor, periphery fatigue are mainly in physical labourer, and central nerve fatigue is mainly in brain worker.Fatigue especially central nerve fatigue is long-term to be obtained
It will be changed into confirmed fatigue even " overworking death ", the daily life and work of severe jamming patient less than effective treatment.Mesh
Preceding anti-fatigue medicament clinically is severely restricted due to the adverse reaction such as side effect, additive, excitability.Therefore,
The anti-fatigue medicament for seeking high-efficiency low-toxicity is still the challenge and problem that world medical circle faces.
Roxburgh anoectochilus terminal bud is Fujian platform characteristic Chinese herbal medicine, from orchid family (Orchidaceae) Anoectochilus Blume Anoectochilus
Blume plant Anoectochilus Roxburghiis, all herbal medicine, having clearing heat and cooling blood, expelling wind and removing dampness, removing toxic substances and other effects, (Huang has continuous heavy rain Fujian Chinese medicine
Material standard Foochow:Sea wind publishing house, 2006:154.), it is used for treating the diseases such as hypertension, diabetes, hepatitis and tumour, curative effect
It is favourable, therefore roxburgh anoectochilus terminal bud is known as the great fame such as " king of medicine ", " gold grass " among the people.In addition to Anoectochilus Roxburghii, Taiwan anoectochilus formosanus, Xingren gold
Line is blue, beautiful flower bud Shorthairy Antenoron etc. it is a variety of belong to together with relative genus plant, because it is similar to roxburgh anoectochilus terminal bud Medicinal Materials Characters and chemical composition,
Therefore among the people more as roxburgh anoectochilus terminal bud medicinal material (Zhang Tie, Wan Jing, Mu Jianhua Preliminary Survey of Anoectochilus Bl. Germplasm in Wenshan, Yunnan Province texts
Mountain higher junior college of normal school journal, 2005,18 (1):26-28；Zheng Chun, Huang Yizhong, Ji Lianfang roxburgh anoectochilus terminal buds textual research, original
Plant and commercial investigation Chinese herbal medicines, 1996,27 (3):169-171.).Roxburgh anoectochilus terminal bud class medicinal material mainly contains lactone glycoside (such as gold
Line lotus glycosides), polysaccharide and flavones active component, it is especially higher with anoectochilus roxburghii glycosides and polyoses content.Modern pharmacological research shows,
Anoectochilus roxburghii glycosides are one of main active components of roxburgh anoectochilus terminal bud, have immunological regulation (Xiang M, Liu T, Tan W, Ren H, Li
H,Liu J,Cao H,Cheng Q,Liu X,Zhu H,Tuo Y,Wang J,Zhang Y.Effects of
kinsenoside,a potential immunosuppressive drug for autoimmune hepatitis,on
dendritic cells/CD8+T cells communication in mice.Hepatology.2016,64(6):2135-
2150.), hypoglycemic (Zhang Y, Cai J, Ruan H, Pi H, Wu J.Antihyperglycemic activity of
kinsenoside,a high yielding constituent from Anoectochilus roxburghii in
streptozotocin diabetic rats.J Ethnopharmacol.2007,114(2):141-145.), liver protection
(Hsieh WT,Tsai CT,Wu JB,Hsiao HB,Yang LC,Lin WC.Kinsenoside,a high yielding
constituent from Anoectochilus formosanus,inhibits carbon tetrachloride
induced Kupffer cells mediated liver damage.Journal of
Ethnopharmacology.2011,135(2):440-449.), reducing blood lipid (Rehman SU, Choi MS, Kim IS, Luo
Z,Xue Y,Yao G,Zhang Y,Yoo HH.In Vitro Assessment of CYP-Mediated Drug
Interactions for Kinsenoside,an Antihyperlipidemic Candidate.Molecules.2016,
21(6).pii:E800.), anti-inflammatory (Hsiao HB1, Hsieh CC, Wu JB, Lin H1, Lin WC.Kinsenoside
inhibits the inflammatory mediator release in a type-II collagen induced
arthritis mouse model by regulating the T cells responses.BMC Complement
Altern Med.2016,16:And anti-osteoporosis (Hsiao HB, Lin H, Wu JB, Lin WC.Kinsenoside 80)
prevents ovariectomy-induced bone loss and suppresses osteoclastogenesis by
regulating classical NF-κB pathways.Osteoporos Int.2013,24(5):It is 1663-1676.) etc. raw
Thing activity, but so far there are no close the report of roxburgh anoectochilus terminal bud and its monomeric compound anoectochilus roxburghii glycosides for antifatigue aspect.
The content of the invention
It is an object of the invention to provide the new medical usage of anoectochilus roxburghii glycosides.
The first aspect of the present invention, there is provided application of the anoectochilus roxburghii glycosides in anti-fatigue medicament or food is prepared.
The chemical constitution of described anoectochilus roxburghii glycosides is as follows：
The second aspect of the present invention, there is provided a kind of anti-fatigue medicament or food, in described anti-fatigue medicament or food
Active component is anoectochilus roxburghii glycosides.
Preferably, pharmaceutically acceptable auxiliary material is also included in described anti-fatigue medicament or food.
Described anoectochilus roxburghii glycosides, its preparation method comprise the following steps：
(A) extract solution is prepared：After Anoectochilus Blume and its relative genus vegetable drug are crushed, extractor is put into, with 40~
90% ethanol heat carries 2~3 times, and each solvent dosage is about 8~10 times of crude drug amount, and each extraction time is 1~2 hour,
Merge extract solution；
(B) refining and concentrating is dried：The obtained extract solutions of step A are concentrated, concentrate is through ethyl acetate abstraction impurity removal
Afterwards, water layer is concentrated and dried to obtain crude extract；
(C) isolate and purify：The obtained crude extracts of step B are dissolved in loading macroporous absorbent resin after water, collect 0~10% second
Alcohol elution fraction, anoectochilus roxburghii glycosides crude product is concentrated and dried to obtain, loading forward direction silicagel column, using methylene chloride-methanol system elutions, is received
Collection 6:1 elution fraction, obtain anoectochilus roxburghii glycosides (purity>90%).
Preferably, the Anoectochilus Blume described in step A and its relative genus plant be selected from Anoectochilus Roxburghii, Taiwan anoectochilus formosanus,
It is Zhejiang Shorthairy Antenoron, Xingren Shorthairy Antenoron, the southern regions of the Yunnan Province Shorthairy Antenoron, lengthy motion picture Shorthairy Antenoron, Nandan Shorthairy Antenoron, beautiful flower bud Shorthairy Antenoron, high Shorthairy Antenoron, short
Lip Shorthairy Antenoron, variegated leaf be blue, big piebald aspidistra, slight variegated leaf is blue, hair stalk variegated leaf is blue, the full variegated leaf in day is blue and one kind in blood aspidistra or
It is two or more.
Preferably, the concentration described in step B is that solvent is recovered under reduced pressure.
Preferably, the macroreticular resin described in step C is selected from HP-20, AB-8, D-101, ZTC-1, HPD-100 or DA201
In one kind.
The present invention using heavy burden mouse forced swimming test model (Liu Y, Li L, An S, Zhang Y, Feng S, Zhao L,
Teng L,Wang D.Antifatigue Effects of Antrodia cinnamomea Cultured Mycelium
via Modulation of Oxidative Stress Signaling in a Mouse Model.Biomed Res
Int.2017:9374026.) antifatigue effect of anoectochilus roxburghii glycosides gastric infusion provided by the invention, is observed.This animal model is
The antifatigue animal model being widely recognized as, test mainly for exercise induced fatigue, including central fatigue and periphery fatigue (king
Some thinking Chinese experimental pharmacology of traditional Chinese medical formulae magazines of animal model during Cong Xiao, all army are studied Chinese medicine resisting kinetic fatigue, 2009,
15(3):83-85；The research China Sports Science and Technologies of cloud Animal Model of Exercise Fatigue, 2003,39 (2) are liked in Zheng Lan, land:20-
23；Peak in field knows China Tissue Engineering Study, 2015,19 (42) to maincenter and re-recognizing for peripheric movement fatigue:6849-
6854)。
Experimental result shows, after anoectochilus roxburghii glycosides 100mg/kg continuous gavages are administered 18 days, to mouse normal physiological conditions and
Changes of weight does not make significant difference；Naive mice walking weight load is 1577.7 ± 303.1 seconds, anoectochilus roxburghii glycosides administration group mouse
Walking weight load is 2775.2 ± 770.3 seconds, significant difference (p be present with blank group<0.01)；And anoectochilus roxburghii glycosides can also show
Writing reduces BLD (lactic acid) content and the activity for improving LDH (lactic dehydrogenase) in mice serum in swimming with a load attached to the body mice serum,
Illustrate that anoectochilus roxburghii glycosides have good antifatigue effect.
It is of the present invention antifatigue, refer to prevent or treat periphery fatigue or central nerve fatigue, improve body work capacity,
Reduce the content of lactic acid in serum and improve activity etc. of lactic dehydrogenase in serum, improve myalgia, it is absent minded,
The symptoms such as decrease of memory.
The present invention has searched out preferable anti-fatigue medicament.The invention provides the new medical usage of anoectochilus roxburghii glycosides, through medicine
Effect learns research and shows that anoectochilus roxburghii glycosides of the invention can significantly extend heavy burden mouse swimming time, antifatigue significant effect, can be used for
Prepare anti-fatigue medicament or food.The present invention provides a kind of new source to seek new anti-fatigue medicament.
Brief description of the drawings
Fig. 1 is that the weight of animals changes over time figure；
Fig. 2 is influence (mean ± SD, n=10) of the anoectochilus roxburghii glycosides to heavy burden mouse swimming time, wherein * * vs blank pair
According to group, p<0.01；
Fig. 3 is the anoectochilus roxburghii glycosides influence (mean ± SD, n=10) horizontal to BLD (lactic acid) in swimming with a load attached to the body mice serum,
Wherein * * vs blank control groups, p<0.01；
Fig. 4 is anoectochilus roxburghii glycosides influence (mean ± SD, the n horizontal to LDH (lactic dehydrogenase) in swimming with a load attached to the body mice serum
=10), wherein * vs blank control groups, p<0.05.
Embodiment
Embodiment provided by the invention is elaborated with reference to embodiment and accompanying drawing.
Embodiment 1：The preparation of anoectochilus roxburghii glycosides
(1) extract solution is prepared：After Anoectochilus Blume and its relative genus vegetable drug are crushed, extractor is put into, with 40~
90% ethanol heat carries 2~3 times, and each solvent dosage is about 8~10 times of crude drug amount, and each extraction time is 1~2 hour,
Merge extract solution；
(2) refining and concentrating is dried：Said extracted liquid is concentrated, concentrate is after ethyl acetate abstraction impurity removal, by water
Layer is concentrated and dried to obtain crude extract；
(3) isolate and purify：Above-mentioned crude extract is dissolved in loading macroporous absorbent resin after water, collects 0~10% ethanol elution
Part, anoectochilus roxburghii glycosides crude product is concentrated and dried to obtain, loading forward direction silicagel column, using methylene chloride-methanol system elutions, collects 6:1
Elution fraction, obtain anoectochilus roxburghii glycosides (purity>90%).
Embodiment 2：The antifatigue pharmacodynamic experiment of anoectochilus roxburghii glycosides
2.1 experiment material
18~22g ICR mouse (The 2nd Army Medical College Experimental Animal Center provides) 20, mouse LDH (lactic dehydrogenase)
With BLA (blood lactase acid) kit (Bioengineering Research Institute is built up in Nanjing).Key instrument：5L beakers (Shanghai standing grain vapour glass apparatus
Co., Ltd), ELIASA (BIO-RAD 550), centrifuge (Labofuge 400R, Heraeus companies), electronic balance (FA
110A types, Shanghai Precision Scientific Apparatus Co., Ltd).
2.2 animal packets and experiment process
20 mouse are weighed before experiment, 2 groups are randomly divided into by weight, every group 10, free diet, water inlet.It is divided into
Blank group and anoectochilus roxburghii glycosides group.Blank group：Give and administration group same volume physiological saline；Anoectochilus roxburghii glycosides group：Gastric infusion, often
Day once, dosage 100mg/kg, successive administration 18d.
2.3 observation index
(1) each group Mice Body quality change situation is recorded, is weighed once within every 9 days.
(2) after last day gavage 30min, 6% (lead) that born a heavy burden at mouse root of the tail allows it to start to swim in 5L beakers
Swimming to head in 7s can not emerge timing stop, being calculated as power and exhaust the time.
(3) mouse is taken out, eyeball is dialled and takes blood.4 DEG C of blood, 3500r/min are centrifuged 10 minutes, take supernatant to preserve -80 DEG C.Root
Time-and-motion study BLA, LDH are carried out according to kit specification.
2.4 experimental result
The analysis of two sample t test statisticses, P are carried out to two groups of data using SPSS13.0<Think that difference has system when 0.05
Meter learns meaning.
(1) mouse weight changes
Naive mice body weight gradually rises, and mouse weight is substantially steady after anoectochilus roxburghii glycosides administration processing, and blank group ratio
Compared with there was no significant difference, illustrate that anoectochilus roxburghii glycosides do not have influence substantially on mouse normal physiological conditions, as shown in Figure 1.
(2) the mice burden swimming time
As shown in Fig. 2 naive mice heavy burden swimming time is 1577.7 ± 303.1 seconds, anoectochilus roxburghii glycosides administration group
Mouse power exhausted walking weight load for 2775.2 ± 770.3 seconds, significant difference (p be present with blank group<0.01) gold thread, is shown
Lotus glycosides can dramatically increase the mice burden swimming time.
(3) Serum Indexes
As shown in Figure 3, Figure 4, anoectochilus roxburghii glycosides group p compared with blank group<0.05, anoectochilus roxburghii glycosides can significantly reduce mice serum
Middle lactic acid BLA content and the activity for improving lactate dehydrogenase L DH in mice serum, illustrate anoectochilus roxburghii glycosides 100m g/kg gavages
The tired correlation factor that administration can be significantly improved in heavy burden mouse model serum is horizontal.
In view of above-mentioned effect experiment result, anoectochilus roxburghii glycosides of the invention have notable anti-fatigue active, therefore can be used for making
Standby anti-fatigue medicament or food.
The preferred embodiment to the invention is illustrated above, but the invention be not limited to it is described
Embodiment, those skilled in the art can also make a variety of equivalent on the premise of without prejudice to the invention spirit
Modification or replacement, these equivalent modifications or replacement are all contained in the application claim limited range.
Claims (7)
1. application of the anoectochilus roxburghii glycosides in anti-fatigue medicament or food is prepared.
2. a kind of anti-fatigue medicament or food, it is characterised in that the active component in described anti-fatigue medicament or food is gold
Line lotus glycosides.
3. anti-fatigue medicament according to claim 2 or food, it is characterised in that in described anti-fatigue medicament or food
Also include pharmaceutically acceptable auxiliary material.
4. anti-fatigue medicament according to claim 2 or food, it is characterised in that the preparation method of described anoectochilus roxburghii glycosides
Comprise the following steps：
(A) extract solution is prepared：After Anoectochilus Blume and its relative genus vegetable drug are crushed, extractor is put into, with 40~90%
Ethanol heat carries 2~3 times, and each solvent dosage is about 8~10 times of crude drug amount, and each extraction time is 1~2 hour, and merging carries
Take liquid；
(B) refining and concentrating is dried：The obtained extract solutions of step A are concentrated, concentrate, will after ethyl acetate abstraction impurity removal
Water layer is concentrated and dried to obtain crude extract；
(C) isolate and purify：The obtained crude extracts of step B are dissolved in loading macroporous absorbent resin after water, 0~10% ethanol is collected and washes
De- part, anoectochilus roxburghii glycosides crude product is concentrated and dried to obtain, loading forward direction silicagel column, using methylene chloride-methanol system elutions, collects 6:
1 elution fraction, obtains anoectochilus roxburghii glycosides.
5. anti-fatigue medicament according to claim 4 or food, it is characterised in that Anoectochilus Blume described in step A and
Its relative genus plant is selected from Anoectochilus Roxburghii, Taiwan anoectochilus formosanus, Zhejiang Shorthairy Antenoron, Xingren Shorthairy Antenoron, the southern regions of the Yunnan Province Shorthairy Antenoron, lengthy motion picture gold
Line orchid, Nandan Shorthairy Antenoron, beautiful flower bud Shorthairy Antenoron, high Shorthairy Antenoron, brachycheilia Shorthairy Antenoron, variegated leaf be blue, big piebald aspidistra, slight variegated leaf are blue,
It is more than one or both of hair stalk variegated leaf orchid, the full variegated leaf orchid in day and blood aspidistra.
6. anti-fatigue medicament according to claim 4 or food, it is characterised in that the concentration described in step B is decompression
Recycling design.
7. anti-fatigue medicament according to claim 4 or food, it is characterised in that the macroreticular resin choosing described in step C
From one kind in HP-20, AB-8, D-101, ZTC-1, HPD-100 or DA201.
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Cited By (5)
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| CN109010609A (en) * | 2018-09-30 | 2018-12-18 | 华中科技大学 | A kind of preparation method of the Anoectochilus containing anoectochilus roxburghii glycosides and the application of extract |
| CN110585227A (en) * | 2019-11-14 | 2019-12-20 | 江西厚朴贯众科技有限公司 | Application of anoectochilus formosanus glycoside in preparation of medicine for preventing and treating neuroinflammation |
| CN110585225A (en) * | 2019-11-14 | 2019-12-20 | 江西厚朴贯众科技有限公司 | Application of Anoectochilus roxburghii glycoside in preparation of medicine for removing brain beta amyloid plaque |
| CN112843177A (en) * | 2021-02-24 | 2021-05-28 | 中国人民解放军海军军医大学 | Application of anoectochilus formosanus extract in preparation of medicine or health-care product for treating or relieving pain |
| CN113372462A (en) * | 2021-06-03 | 2021-09-10 | 南昌大学 | Method for extracting high-purity anemone's-chinensis polysaccharide |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109010609A (en) * | 2018-09-30 | 2018-12-18 | 华中科技大学 | A kind of preparation method of the Anoectochilus containing anoectochilus roxburghii glycosides and the application of extract |
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| CN110585225A (en) * | 2019-11-14 | 2019-12-20 | 江西厚朴贯众科技有限公司 | Application of Anoectochilus roxburghii glycoside in preparation of medicine for removing brain beta amyloid plaque |
| CN112843177A (en) * | 2021-02-24 | 2021-05-28 | 中国人民解放军海军军医大学 | Application of anoectochilus formosanus extract in preparation of medicine or health-care product for treating or relieving pain |
| CN113372462A (en) * | 2021-06-03 | 2021-09-10 | 南昌大学 | Method for extracting high-purity anemone's-chinensis polysaccharide |
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