CN107495382A - A kind of purposes of red seaweed polysaccharide - Google Patents
A kind of purposes of red seaweed polysaccharide Download PDFInfo
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- CN107495382A CN107495382A CN201710698300.5A CN201710698300A CN107495382A CN 107495382 A CN107495382 A CN 107495382A CN 201710698300 A CN201710698300 A CN 201710698300A CN 107495382 A CN107495382 A CN 107495382A
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- seaweed polysaccharide
- red seaweed
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- 150000004676 glycans Chemical class 0.000 title claims abstract description 78
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 78
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 78
- 241001474374 Blennius Species 0.000 title claims abstract description 58
- 238000002360 preparation method Methods 0.000 claims abstract description 20
- 241000206572 Rhodophyta Species 0.000 claims abstract description 17
- 239000002994 raw material Substances 0.000 claims abstract description 16
- 235000020510 functional beverage Nutrition 0.000 claims abstract description 8
- 235000013406 prebiotics Nutrition 0.000 claims abstract description 8
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 42
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 28
- 238000000605 extraction Methods 0.000 claims description 22
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 19
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 19
- 239000006228 supernatant Substances 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- LPQOADBMXVRBNX-UHFFFAOYSA-N ac1ldcw0 Chemical compound Cl.C1CN(C)CCN1C1=C(F)C=C2C(=O)C(C(O)=O)=CN3CCSC1=C32 LPQOADBMXVRBNX-UHFFFAOYSA-N 0.000 claims description 8
- 230000000694 effects Effects 0.000 claims description 8
- 238000006243 chemical reaction Methods 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 7
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 6
- 241000649992 Digenea simplex Species 0.000 claims description 4
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 4
- 238000001556 precipitation Methods 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- 239000002904 solvent Substances 0.000 claims description 4
- 229920001817 Agar Polymers 0.000 claims description 3
- 241000206649 Batrachospermum Species 0.000 claims description 3
- 239000008272 agar Substances 0.000 claims description 3
- 241000195493 Cryptophyta Species 0.000 claims description 2
- FFBHFFJDDLITSX-UHFFFAOYSA-N benzyl N-[2-hydroxy-4-(3-oxomorpholin-4-yl)phenyl]carbamate Chemical compound OC1=C(NC(=O)OCC2=CC=CC=C2)C=CC(=C1)N1CCOCC1=O FFBHFFJDDLITSX-UHFFFAOYSA-N 0.000 claims description 2
- 229920001184 polypeptide Polymers 0.000 claims description 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims 1
- 238000005265 energy consumption Methods 0.000 abstract description 5
- 230000036737 immune function Effects 0.000 abstract description 4
- 230000009385 viral infection Effects 0.000 abstract description 4
- 238000005516 engineering process Methods 0.000 abstract description 3
- 208000036142 Viral infection Diseases 0.000 abstract description 2
- 239000000654 additive Substances 0.000 abstract description 2
- 230000000996 additive effect Effects 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 description 9
- 210000004027 cell Anatomy 0.000 description 5
- 238000011084 recovery Methods 0.000 description 4
- 210000000170 cell membrane Anatomy 0.000 description 3
- 230000002708 enhancing effect Effects 0.000 description 3
- 238000005189 flocculation Methods 0.000 description 3
- 230000016615 flocculation Effects 0.000 description 3
- DGLAHESNTJWGDO-SRVKXCTJSA-N His-Ser-His Chemical compound C1=C(NC=N1)C[C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CC2=CN=CN2)C(=O)O)N DGLAHESNTJWGDO-SRVKXCTJSA-N 0.000 description 2
- 102000019197 Superoxide Dismutase Human genes 0.000 description 2
- 108010012715 Superoxide dismutase Proteins 0.000 description 2
- 150000001413 amino acids Chemical group 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 235000019628 coolness Nutrition 0.000 description 2
- 239000012043 crude product Substances 0.000 description 2
- 108010040030 histidinoalanine Proteins 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 210000005259 peripheral blood Anatomy 0.000 description 2
- 239000011886 peripheral blood Substances 0.000 description 2
- 230000001376 precipitating effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000003809 water extraction Methods 0.000 description 2
- DECCMEWNXSNSDO-ZLUOBGJFSA-N Ala-Cys-Ala Chemical compound C[C@H](N)C(=O)N[C@@H](CS)C(=O)N[C@@H](C)C(O)=O DECCMEWNXSNSDO-ZLUOBGJFSA-N 0.000 description 1
- OBVSBEYOMDWLRJ-BFHQHQDPSA-N Ala-Gly-Thr Chemical compound C[C@@H](O)[C@@H](C(O)=O)NC(=O)CNC(=O)[C@H](C)N OBVSBEYOMDWLRJ-BFHQHQDPSA-N 0.000 description 1
- CVOZXIPULQQFNY-ZLUOBGJFSA-N Cys-Ala-Cys Chemical compound C[C@H](NC(=O)[C@@H](N)CS)C(=O)N[C@@H](CS)C(O)=O CVOZXIPULQQFNY-ZLUOBGJFSA-N 0.000 description 1
- WTNLLMQAFPOCTJ-GARJFASQSA-N Cys-His-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CC2=CN=CN2)NC(=O)[C@H](CS)N)C(=O)O WTNLLMQAFPOCTJ-GARJFASQSA-N 0.000 description 1
- WZJLBUPPZRZNTO-CIUDSAMLSA-N Cys-Ser-Lys Chemical compound C(CCN)C[C@@H](C(=O)O)NC(=O)[C@H](CO)NC(=O)[C@H](CS)N WZJLBUPPZRZNTO-CIUDSAMLSA-N 0.000 description 1
- MHXKHKWHPNETGG-QWRGUYRKSA-N Gly-Lys-Leu Chemical compound [H]NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(O)=O MHXKHKWHPNETGG-QWRGUYRKSA-N 0.000 description 1
- 229920002527 Glycogen Polymers 0.000 description 1
- 229920000869 Homopolysaccharide Polymers 0.000 description 1
- DSSOYPJWSWFOLK-CIUDSAMLSA-N Ser-Cys-Leu Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(C)C)C(O)=O DSSOYPJWSWFOLK-CIUDSAMLSA-N 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- DCOOGDCRFXXQNW-ZKWXMUAHSA-N Val-Asn-Cys Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CS)C(=O)O)N DCOOGDCRFXXQNW-ZKWXMUAHSA-N 0.000 description 1
- 235000010489 acacia gum Nutrition 0.000 description 1
- 239000001785 acacia senegal l. willd gum Substances 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 238000013475 authorization Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 229930182478 glucoside Natural products 0.000 description 1
- 150000008131 glucosides Chemical class 0.000 description 1
- 229940096919 glycogen Drugs 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 230000002434 immunopotentiative effect Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000013067 intermediate product Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 150000002972 pentoses Chemical class 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 235000019605 sweet taste sensations Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Molecular Biology (AREA)
- Sustainable Development (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Organic Chemistry (AREA)
- Mycology (AREA)
- Medicines Containing Plant Substances (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
The invention belongs to functional beverage additive technology field, disclose a kind of purposes of red seaweed polysaccharide, for being used as the purposes of prebiotics in functional beverage, the red seaweed polysaccharide of the present invention is used for the addition for making prebiotics in drink with function, colony balance in body can be effectively facilitated, it can significantly improve and improve the nonspecific cell of body of people and specific humoral immune function, strengthen human body and resist a variety of viral infection abilities, red algae raw material sources of the present invention are stable, prepared red seaweed polysaccharide purity is high, quality is good, and preparation process is simple, operation facility, energy consumption is low, red algae raw material resources are using fully, meet green requirement.
Description
Technical field
The invention belongs to functional beverage additive technology field, and in particular to a kind of purposes of red seaweed polysaccharide.
Background technology
Polysaccharide（polysaccharide）The sugar chain combined by glycosidic bond, monose composition that at least will be more than 10 it is poly-
Sugar high molecular carbohydrate is closed, formula (C6H10O5) n can be used to represent.The polysaccharide being made up of identical monose is referred to as homopolysaccharide,
Such as starch, cellulose and glycogen；Heteroglycan is referred to as with the polysaccharide that different monose forms, if Arabic gum is by pentose and gala
Sugar etc. forms.Polysaccharide is not a kind of pure chemical substance, but the mixture of the different material of extent of polymerization.Polysaccharide is general
It is not soluble in water, no sweet taste, it is impossible to form crystallization, no reproducibility and mutarotation.Polysaccharide is also glucosides, it is possible to hydrolyzes,
In hydrolytic process, a series of intermediate product is often produced, final complete hydrolysis obtains monose.Polysaccharide exists as immunopotentiating agent
Clinically have been widely used, because therefore the extraction of polysaccharide has huge value.
Prior art such as, Chinese invention patent Granted publication document, Authorization Notice No. CN1157416C, the invention
The method of flocculation extraction algal polysaccharides：The extraction containing algal polysaccharides is made using cyclic ultrasonic breaking or water-boiling method extraction first
Feed liquid, the extraction feed liquid containing algal polysaccharides is inserted in reactor, adjust 20-60 DEG C of reactor temperature, pH 5-8；In 300-
Under 1500rpm mixing speeds, flocculant is added, the feed liquid containing algal polysaccharides extraction is flocculated or flocculation of electric field extracts, is obtained
Algal polysaccharides crude product, is purified or drying and processing to algal polysaccharides crude product again afterwards, that is, algal polysaccharides product is made, this
Invention flocculation dosage used is few, and flocculant cost is only 1/10 or so of ethanol cost, so as to substantially reduce algal polysaccharides
Extraction cost, after flocculant adds in the extracting method of the invention, algal polysaccharides can not be flocculated extraction completely so that raw material
Utilization rate is not high.
The content of the invention
It is an object of the invention to provide a kind of preparation process is simple, preparation process energy consumption is low, prepares recovery rate height, is used for
It is used as a kind of purposes of red seaweed polysaccharide of prebiotics addition in functional beverage.
The present invention is for the scheme taken of solution above-mentioned technical problem：
A kind of purposes of red seaweed polysaccharide, red seaweed polysaccharide are used for the purposes of prebiotics in functional beverage, and red seaweed polysaccharide can be significantly
Improve cellular superoxide dismutase（SOD）Vigor, promote human peripheral blood in NR cells activity, can also significantly improve
With the nonspecific cell of body for improving people and specific humoral immune function, a variety of virus infection energy of enhancing human body resistance
Power.
Preferably, red algae raw material is the one or more in seaweed, agar, Digenea simplex, batrachos-permum, coralline algae, institute
The red algae raw material sources of choosing are stable, and cost is cheap.
Preferably, the monose composition in red seaweed polysaccharide be galactolipin and 3,6- inner ether galactolipin, sulfate content for 15 ~
18%, the composition of monose is stable, and sulfate content is suitable so that red seaweed polysaccharide has good anti-oxidant, antiviral, anti-inflammatory etc.
Effect.
Preferably, the preparation of red seaweed polysaccharide comprises the following steps：Red algae raw material is taken to clean segment, add 0.3 ~
0.4mol/L NaOH and active peptides mixed liquor, stirring extraction, after mixed liquor cooling, the HCL for adding 4.5 ~ 6.4mol/L is molten
Liquid, centrifugal treating obtain supernatant B, and supernatant B is concentrated, and add the edible ethanol of 2.6 ~ 4 times of volumes afterwards, and standing takes precipitation, obtained
Red seaweed polysaccharide coarse fodder；It is dissolved in water, after adding solvent under water-bath, 9 ~ 11%H is at the uniform velocity added dropwise2O2Solution, after the completion of reaction, surpassed
Filter is handled, and obtains pure red seaweed polysaccharide, and this preparation method condition requires low, and simple to operate, preparation process energy consumption is low, the utilization of raw material
It is high.
Preferably, NaOH solution and the addition of active peptides mixed liquor are the 12 ~ 24 of red algae raw material in preparation process 1
Times w/v, wherein the weight ratio of NaOH solution and active peptides are 1:0.02 ~ 0.04, the amino acid sequence of active peptides
For：HSHACACSKHSHAGTASCLCHPGKLCACVNCSR, stir extraction temperature be 55 DEG C ~ 63 DEG C, extraction time be 1.8 ~
2.3h, in extraction process is stirred, the action effect of NaOH solution is limited, and after adding active peptides, active peptides and NaOH are molten
Liquid collective effect, the rupture of cell membrane and the release of polysaccharide can be greatly promoted, reduce the resistance of hot water extraction, reduce extraction
Time, red seaweed polysaccharide recovery rate is improved, make full use of red algae resource.
Preferably, the pH value that supernatant B is made in preparation process 2 is 2.4 ~ 2.8, the control of pH value not only increases molten
Liquid stability, also it can provide favorable environment for subsequent reactions.
Preferably, be concentrated into supernatant B water content in preparation process 3 as 60 ~ 80%, the concentration of edible ethanol for 92 ~
The addition of ethanol capable of reducing using after 96%, supernatant B concentration, and polysaccharide is dissolved in hot water and does not dissolve in high concentration ethanol, control
After the concentration using ethanol of addition, red seaweed polysaccharide can be made to be separated out in the form of precipitating from water, and edible ethanol is nontoxic,
Red seaweed polysaccharide property is not influenceed so that obtained red seaweed polysaccharide purity is high, and quality is good.
Preferably, the composition of solvent and its parts by weight are in preparation process 4：1 ~ 3 part of 0.4mol/L NaCl solutions,
1.6 ~ 2.3 parts of 0.6mol/L NaAc solution, 0.8 ~ 1.5 part of 3.5mol/L acetic acid, H2O2Solution addition is 1.2 ~ 2.4 parts, profit
With the reaction between chemical substance, the impurity in red seaweed polysaccharide, and rapid reaction, the red seaweed polysaccharide purity of gained can be effectively removed
It is high.
Compared with prior art, beneficial effects of the present invention are：The red seaweed polysaccharide of the present invention is used to make in drink with function
The addition of prebiotics, colony balance in body can be effectively facilitated, the body that can significantly improve and improve people is nonspecific
Cell and specific humoral immune function, enhancing human body resist a variety of viral infection abilities, and red algae of the present invention is former
Expect steady sources, prepared red seaweed polysaccharide purity is high, and quality is good, and preparation process is simple, and operation facility, energy consumption is low,
The addition of active peptides can greatly promote the rupture of cell membrane and the release of polysaccharide, reduce extraction time, it is more to improve red algae
Sugared recovery rate, red algae resource is made full use of, meet green requirement.
Embodiment
It is described in further detail with reference to embodiments：
Embodiment 1：
A kind of purposes of red seaweed polysaccharide, red seaweed polysaccharide are used for the purposes of prebiotics in functional beverage, and red seaweed polysaccharide can be significantly
Improve cellular superoxide dismutase（SOD）Vigor, promote human peripheral blood in NR cells activity, can also significantly improve
With the nonspecific cell of body for improving people and specific humoral immune function, a variety of virus infection energy of enhancing human body resistance
Power.The preparation method of red seaweed polysaccharide comprises the following steps：Take red algae raw material to clean segment, add 0.35mol/L NaOH and activity
The addition of polypeptide mixed liquor, NaOH solution and active peptides mixed liquor is 12 ~ 24 times of w/vs of red algae raw material, wherein
NaOH solution and the weight of active peptides ratio are 1:0.026, the amino acid sequence of active peptides is：
HSHACACSKHSHAGTASCLCHPGKLCACVNCSR, stirring extraction, the temperature for stirring extraction is 58 DEG C, and extraction time is
2.1h, mixed liquor A is obtained, after adding active peptides, active peptides and NaOH solution collective effect, the broken of cell membrane can be greatly promoted
The release with polysaccharide is split, the resistance of hot water extraction is reduced, reduces extraction time, improves red seaweed polysaccharide recovery rate, is made red
Algae resource makes full use of；After liquid A coolings to be mixed, the pH value for adding 5.3mol/L HCL solution to mixed liquor is 2.5, centrifugation
Handle to obtain supernatant B；It is 68% to take supernatant B to be concentrated into water content, adds 95% edible ethanol of 3.3 times of volumes afterwards, standing takes
Precipitation, obtains red seaweed polysaccharide coarse fodder, polysaccharide is dissolved in hot water and does not dissolve in high concentration ethanol, controls the concentration using ethanol of addition
Afterwards, red seaweed polysaccharide can be made to be separated out in the form of precipitating from water, and edible ethanol is nontoxic, does not influence red seaweed polysaccharide property,
So that obtained red seaweed polysaccharide purity is high, quality is good；Finally take red seaweed polysaccharide coarse fodder to be dissolved in water, added under water-bath
After 2 parts of 0.4mol/L NaCl solutions, 1.7 parts of 0.6mol/L NaAc solution, 1.2 parts of 3.5mol/L acetic acid, 10.5% is at the uniform velocity added dropwise
H2O21.9 parts of solution, after the completion of reaction, carry out hyperfiltration treatment, obtain pure red seaweed polysaccharide, wherein, red algae raw material be seaweed, agar,
Digenea simplex, the monose composition in the pure red seaweed polysaccharide of gained be galactolipin and 3,6- inner ether galactolipin, and sulfate content is 16%.
The prior art of routine techniques dawn known to those skilled in the art in the present embodiment, is not chatted in detail herein
State.
Embodiment 2：
A kind of purposes of red seaweed polysaccharide, red seaweed polysaccharide are used for the purposes of prebiotics in functional beverage.
The preparation method of red seaweed polysaccharide comprises the following steps：
1）Take Digenea simplex and batrachos-permum raw material to clean segment, add the 0.3 ~ 0.4mol/L NaOH and active peptides of 21 times of volumes
The weight ratio of mixed liquor, wherein NaOH solution and active peptides is 1:0.033, under the conditions of temperature is 61 DEG C, stirring extraction 2h,
Obtain mixed liquor A；
2）After liquid A coolings to be mixed, HCL solution to the mixed liquor pH value for adding 4.8mol/L is 2.6, and centrifugal treating obtains supernatant
B；
3）It is 67% to take supernatant B to be concentrated into water content, and the concentration for adding 2.6 ~ 4 times of volumes afterwards is 93% edible ethanol, and standing takes
Precipitation, obtains red seaweed polysaccharide coarse fodder；
4）Take red seaweed polysaccharide coarse fodder to be dissolved in water, it is molten that 1.3 parts of 0.4mol/L NaCl solutions, 0.6mol/L NaAc are added under water-bath
1.8 parts of liquid, 1.3 parts of 3.5mol/L acetic acid, are at the uniform velocity added dropwise 10.7%H2O21.8 parts of solution, after the completion of reaction, hyperfiltration treatment is carried out,
Pure red seaweed polysaccharide is obtained, this preparation method condition requires low, and simple to operate, preparation process energy consumption is low, and raw material utilizes height.
The prior art of routine techniques dawn known to those skilled in the art in the present embodiment, is not chatted in detail herein
State.
Specific embodiment described herein is only to present invention explanation for example.The technical field of the invention
Technical staff can be made various modifications or supplement to described specific embodiment or be substituted using similar mode, but
Without departing from the present invention's or surmount scope defined in appended claims.
SEQUENCE LISTING
<110>The Gothic Bioisystech Co., Ltd of Lanxi City
<120>A kind of purposes of red seaweed polysaccharide
<130> 1
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 33
<212> PRT
<213>It is artificial synthesized
<400> 1
His Ser His Ala Cys Ala Cys Ser Lys His Ser His Ala Gly Thr Ala
1 5 10 15
Ser Cys Leu Cys His Pro Gly Lys Leu Cys Ala Cys Val Asn Cys Ser
20 25 30
Arg
Claims (8)
1. a kind of purposes of red seaweed polysaccharide, it is characterised in that the red seaweed polysaccharide is used for the purposes of prebiotics in functional beverage.
A kind of 2. purposes of red seaweed polysaccharide according to claim 1, it is characterised in that described red algae raw material be seaweed,
One or more in agar, Digenea simplex, batrachos-permum, coralline algae.
A kind of 3. purposes of red seaweed polysaccharide according to claim 1, it is characterised in that the monose in described red seaweed polysaccharide
It is 15 ~ 18% to form as galactolipin and 3,6- inner ether galactolipins, sulfate content.
A kind of 4. purposes of red seaweed polysaccharide according to claim 1, it is characterised in that the preparation side of the red seaweed polysaccharide
Method comprises the following steps：Take red algae raw material to clean segment, add 0.3 ~ 0.4mol/L NaOH and active peptides mixed liquor, stirring
Extraction, after mixed liquor cooling, 4.5 ~ 6.4mol/L HCL solution being added, centrifugal treating obtains supernatant B, supernatant B is concentrated,
The edible ethanol of 2.6 ~ 4 times of volumes is added afterwards, and standing takes precipitation, obtains red seaweed polysaccharide coarse fodder；It is dissolved in water, solvent is added under water-bath
Afterwards, 9 ~ 11%H is at the uniform velocity added dropwise2O2Solution, after the completion of reaction, hyperfiltration treatment is carried out, obtains pure red seaweed polysaccharide.
A kind of 5. purposes of red seaweed polysaccharide according to claim 4, it is characterised in that NaOH in described preparation process 1
The addition of solution and active peptides mixed liquor is 12 ~ 24 times of w/vs of red algae raw material, wherein NaOH solution and activity
The weight ratio of polypeptide is 1:0.02 ~ 0.04, the amino acid sequence of active peptides is：
HSHACACSKHSHAGTASCLCHPGKLCACVNCSR, stir extraction temperature be 55 DEG C ~ 63 DEG C, extraction time be 1.8 ~
2.3h。
6. the purposes of a kind of red seaweed polysaccharide according to claim 4, it is characterised in that be made in described preparation process 2
Supernatant B pH value is 2.4 ~ 2.8.
7. the purposes of a kind of red seaweed polysaccharide according to claim 4, it is characterised in that concentrated in described preparation process 3
It is 60 ~ 80% to supernatant B water content, the concentration of edible ethanol is 92 ~ 96%.
A kind of 8. purposes of red seaweed polysaccharide according to claim 4, it is characterised in that solvent in described preparation process 4
Composition and its parts by weight be：1 ~ 3 part of 0.4mol/L NaCl solutions, 1.6 ~ 2.3 parts of 0.6mol/L NaAc solution, 3.5mol/L
0.8 ~ 1.5 part of acetic acid, H2O2Solution addition is 1.2 ~ 2.4 parts.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104095183A (en) * | 2014-06-30 | 2014-10-15 | 中国科学院海洋研究所 | Application of algal polysaccharide |
| CN105294870A (en) * | 2015-06-09 | 2016-02-03 | 深圳海王药业有限公司 | Spriulina polysacchride and preparation method thereof |
-
2017
- 2017-08-15 CN CN201710698300.5A patent/CN107495382A/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104095183A (en) * | 2014-06-30 | 2014-10-15 | 中国科学院海洋研究所 | Application of algal polysaccharide |
| CN105294870A (en) * | 2015-06-09 | 2016-02-03 | 深圳海王药业有限公司 | Spriulina polysacchride and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 中国科学技术协会主编: "《2012-2013生物化学与分子生物学学科发展报告》", 30 April 2004 * |
| 杨红霞: "《饮料加工技术》", 28 February 2015 * |
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