CN107494264B - Method for rooting semiliquidambar cathayensis tissue culture seedling outside bottle - Google Patents
Method for rooting semiliquidambar cathayensis tissue culture seedling outside bottle Download PDFInfo
- Publication number
- CN107494264B CN107494264B CN201710769397.4A CN201710769397A CN107494264B CN 107494264 B CN107494264 B CN 107494264B CN 201710769397 A CN201710769397 A CN 201710769397A CN 107494264 B CN107494264 B CN 107494264B
- Authority
- CN
- China
- Prior art keywords
- rooting
- tissue culture
- seedling
- seedlings
- semiliquidambar cathayensis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Abstract
The invention discloses a method for rooting semiliquidambar cathayensis tissue culture seedlings outside bottles, which comprises the following steps: rejuvenation of tissue culture seedlings, ex-vitro rooting and seedling stage management; the tissue culture rejuvenation technology, the ex-vitro rooting container selection, the matrix selection and disinfection treatment, the ex-vitro rooting technology and the seedling stage environment regulation are applied to the rooting production of the tissue culture seedlings of the semiliquidambar cathayensis, and the rooting rate of the ex-vitro rooting of the obtained tissue culture seedlings of the semiliquidambar cathayensis can reach 90 percent and the survival rate reaches 95 percent. The invention not only improves the transplanting survival rate of the tissue culture rooted seedlings of the semiliquidambar cathayensis, reduces the cost and shortens the seedling culture period, but also provides a new technical method for the rooting of the tissue culture seedlings of the semiliquidambar cathayensis.
Description
Technical Field
The invention relates to the field of ex-vitro rooting of tissue culture seedlings, in particular to an ex-vitro rooting method of semiliquidambar cathayensis tissue culture seedlings.
Background
The Altingia chinensii Mete is evergreen arbor of Hamamelidaceae, and is a national secondary protection plant. The Chinese special species has comprehensive characters between liquidambar and Altingia, and has scientific value for researching the development of the hamamelidaceae system. The wood material is excellent, the rotary planing property is good, and the wood can be used as a rotary planing product. Meanwhile, the bark has the effects of relaxing muscles and tendons, promoting blood circulation, dispelling wind, removing dampness and the like, is used for treating rheumatic arthritis, chronic lumbocrural pain, hemiplegia, traumatic injury and other symptoms, and has obvious curative effect. Because natural forest stands are seriously interfered by human beings and are difficult to propagate naturally, the distribution range is narrower and narrower, so that wild resources are extremely rare. Tissue culture of plants refers to a technique of separating desired tissues, organs or cells, protoplasts, etc. from a plant body, and culturing under artificially controlled conditions by aseptic manipulation to obtain regenerated whole plants or produce other products having economic value. The tissue culture technology of the semiliquidambar cathayensis is gradually matured, and favorable conditions are created for popularization and application of the tree species.
However, compared with cutting seedling, the tissue culture seedling has the problems of more culture procedures, low transplanting survival rate, high seedling cost and the like. Therefore, on the basis of the mature tissue culture and rapid propagation technology of semiliquidambar cathayensis, the technical researches of rejuvenation and ex-bottle rooting of the semiliquidambar cathayensis are carried out, so that the rooting culture links of the tissue culture seedlings are reduced, the seedling culture period is shortened, the seedling cost is reduced, the field transplantation of the tissue culture rooted seedlings of the semiliquidambar cathayensis is simplified, the tissue culture and rapid propagation and industrialized seedling culture technical system of the semiliquidambar cathayensis is further perfected, and the popularization and application of the tissue culture seedlings are promoted, so that the method has important practical significance.
Disclosure of Invention
The invention aims to provide a method for rooting outside a bottle of a tissue culture seedling of semiliquidambar cathayensis, which has the rooting rate of more than 90 percent, does not need seedling hardening, can be directly transplanted into a nutrition bag, and has the survival rate of more than 95 percent.
In order to realize the purpose of the invention, the invention provides a method for rooting tissue culture seedlings of semiliquidambar cathayensis outside a bottle, which comprises the following steps:
rejuvenation of tissue culture seedlings: inoculating the tissue culture seedling of semiliquidambar cathayensis to an improved MS culture medium to induce and culture bud clusters to grow robustly, and obtaining a rejuvenated bottle seedling;
rooting outside the bottle: filling the sterilized seedling substrate into a rooting container, wherein the height of the seedling substrate is 2cm, trowelling and compacting the seedling substrate, thoroughly spraying clear water, poking a plurality of small holes with the depth of 1.0-1.5cm on the seedling substrate, selecting a rejuvenation bottle seedling with the height of 2-3 cm strong buds, cutting off the strong buds, quickly dipping the strong buds with a rooting agent, inserting the strong buds into the small holes of the seedling substrate, and covering the rooting container;
seedling stage management: transferring the rooting container with plantlets inserted in the ex-vitro rooting step to a culture room for culturing for 15 days, controlling the temperature at 26 +/-2 ℃, and turning on light after dark culturing for 2-3 days, wherein the light is irradiated for 10 hours every day; moving to a seedling hardening shed on the 16 th day, and continuing to take root and harden the seedlings, wherein the temperature of the seedling hardening shed is 15-30 ℃, and the humidity is more than or equal to 70%; on day 16-18, from 10 am to 4 pm, the internal and external sunshades are completely unfolded; on the 19 th-22 th day, from 10 am to 4 pm, the sun is shaded and unfolded, then the seedlings are hardened in full light until semi-lignification, the leaves are thickened and become green, and the seedlings can be planted or transplanted in the field.
Further, in the rejuvenation of the tissue culture seedling, the improved MS culture medium is as follows: NH (NH)4NO3500mg/L，KNO31900mg/L，MgSO4·7H2O 370mg/L，KH2PO4225mg/L，CaCl2·2H2O 440mg/L，MnSO4·4H2O16.9mg/L，ZnSO4·7H2O 8.6mg/L，H3BO36.2mg/L，KI 0.83mg/L，Na2MoO4·2H2O 0.25mg/L，CuSO4·5H2O 0.025mg/L，CoCl2·6H2O 0.025mg/L，FeSO4·7H2O 27.8mg/L，Na2-EDTA·2H2O37.3mg/L, glycine 2.0mg/L, thiamine hydrochloride 2.0mg/L, pyridoxine hydrochloride 0.5mg/L, IVB nicotinic acid 2.0mg/L, inositol 100 mg/L.
Further, in the step of rooting outside the bottle, the seedling culture substrate is a mixture of peat, vermiculite and perlite; preferably, the weight ratio of the peat to the vermiculite to the perlite is 3:1: 1.
Further, the method for disinfecting the seedling substrate comprises the following steps: spreading peat, exposing the peat in the sun for 2-3 days for disinfection, bagging the disinfected peat for later use, mixing the disinfected peat, vermiculite and perlite according to a proportion before rooting and transplanting, uniformly mixing the peat, the vermiculite and the perlite to obtain a mixed matrix, disinfecting the mixed matrix by using a 0.3% potassium permanganate solution until the mixed matrix is wet, and closing the disinfected mixed matrix for at least one day to obtain the seedling culture matrix.
Further, the rooting container in the ex-bottle rooting step is a plastic preservation box with a cover; preferably, the specification of the plastic preservation box is 20cm multiplied by 13cm multiplied by 12 cm.
Further, the rooting agent in the ex-vitro rooting step is formed by mixing indolebutyric acid and naphthylacetic acid; preferably, the mass ratio of the indolebutyric acid to the naphthylacetic acid is 3: 2.
Further, the rooting agent in the ex-vitro rooting step is 800 ppm.
Further, the tissue culture seedling is a Min Semiliquidambar cathayensis tissue culture seedling or a Xiao Ye Semiliquidambar cathayensis tissue culture seedling.
The invention adopts the improved MS culture medium, reduces the ammoniacal state N in the formula, improves the content of P, promotes the development of stem branches and roots, induces and controls the growth and the growth of bud clusters, and is convenient for successfully finishing the ex-vitro rooting in the next step.
The rooting container adopts the plastic preservation box with the cover, and the plastic material is light and transparent, so that the humidity, illumination and the like required by the growth and rooting of the bud seedlings are ensured. The specification of the plastic preservation box is 20cm multiplied by 13cm multiplied by 12cm, which not only can be convenient for filling the matrix, but also can be inserted with 150 healthy buds of 100 plants.
The mixture of peat, vermiculite and perlite is selected as the rooting seedling raising substrate, so that the permeability and moisture retention are good, and when the weight ratio of peat, vermiculite and perlite is 3:1:1, the permeability is good. The peat is northeast peat or imported peat, and has good permeability and good quality. The peat is exposed for 2-3 days in the sun and disinfected by sunlight, and the ultraviolet rays of the sun have good bactericidal effects on bacteria, fungi, rickettsia, viruses, algae and the like. The sterilized substrate is sealed for at least one day by the mixed substrate sterilized by 0.3 percent potassium permanganate solution, so that the seedling substrate has a micro fermentation development process, and the sterilization effect and the transplanting survival rate can be improved. The sealing method is to cover a layer of plastic film on the sterilized mixed matrix.
In the management step of the seedling stage, dark culture is performed for 2-3 days to ensure that cells on the wound are yellowed after cutting and bud strengthening and then are converted into cells of a differentiated root system, so that rooting is facilitated, and the growth of the root system is facilitated.
The seedling hardening shed is used for culturing, when the temperature is lower than 15 ℃, the preservation box is opened, and the window parts at the periphery are closed. When the humidity is lower than 70%, the micro-fog spraying increases the humidity in the box and the moisture of the matrix, which is beneficial to the growth of the seedlings.
The tissue culture rejuvenation technology, the ex-vitro rooting container selection, the matrix selection and disinfection treatment, the ex-vitro rooting technology and the seedling stage environment regulation are applied to the rooting production of the tissue culture seedlings of the semiliquidambar cathayensis, and the rooting rate of the ex-vitro rooting of the obtained tissue culture seedlings of the semiliquidambar cathayensis can reach 90 percent and the survival rate reaches 95 percent. The invention not only improves the transplanting survival rate of the tissue culture rooted seedlings of the semiliquidambar cathayensis, reduces the cost and shortens the seedling culture period, but also provides a new technical method for the rooting of the tissue culture seedlings of the semiliquidambar cathayensis.
Detailed Description
The following detailed description of embodiments of the invention is intended to be illustrative of the invention and is not to be construed as limiting the invention. The examples do not specify particular techniques or conditions, and are performed according to the techniques or conditions described in the literature in the art or according to the product specifications. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products commercially available.
Example 1
Rejuvenation of tissue culture seedlings: inoculating the tissue culture seedling of semiliquidambar cathayensis to an improved MS culture medium to induce and culture bud clusters to grow robustly, and obtaining a rejuvenated bottle seedling;
preparing a seedling culture substrate: spreading peat, exposing the peat in the sun for 2-3 days for disinfection, bagging the disinfected peat for later use, mixing the disinfected peat, vermiculite and perlite according to the mass ratio of 3:1:1 before rooting and transplanting, uniformly mixing the peat, the vermiculite and the perlite to obtain a mixed matrix, disinfecting the mixed matrix by using 0.3% potassium permanganate solution until the mixed matrix is wet, and closing the disinfected mixed matrix for one day to obtain a seedling culture matrix;
rooting outside the bottle: filling the sterilized seedling substrate into a rooting container, wherein the height of the seedling substrate is 2cm, trowelling and compacting the seedling substrate, thoroughly spraying clear water, poking a plurality of small holes with the depth of 1.0-1.5cm on the seedling substrate, selecting a rejuvenation bottle seedling with the height of 2-3 cm strong buds, cutting off the strong buds, quickly dipping the strong buds with 800ppm of rooting agent (formed by mixing indolebutyric acid and naphthylacetic acid according to the mass ratio of 3: 2), then inserting the strong buds into the small holes of the seedling substrate, filling the small holes, inserting 100 strong buds into the seedling substrate, and covering the rooting container; the rooting container is a plastic preservation box with a cover and the specification of 20cm multiplied by 13cm multiplied by 12 cm;
seedling stage management: transferring the rooting container with the seedlings inserted into the culture room to culture for 15 days, controlling the temperature at 26 +/-2 ℃, turning on light after dark culture for 2-3 days, and illuminating for 10 hours every day; moving to a seedling hardening shed on the 16 th day, and continuing to take root and harden the seedlings, wherein the temperature of the seedling hardening shed is 15-30 ℃, and the humidity is more than or equal to 70%; on day 16-18, from 10 am to 4 pm, the internal and external sunshades are completely unfolded; on the 19 th-22 th day, from 10 am to about 4 pm, the outside is shaded and unfolded, then the seedlings are hardened in full light until semi-lignification, the leaves are thickened and become green, and then the seedlings can be planted or transplanted in a field, and the rooting rate and survival rate of the seedlings are counted.
Experiments show that 95 of the semiliquidambar cathayensis seedlings contain more than 3 root systems, 90 of the semiliquidambar cathayensis seedlings grow well, and 10 leaves turn yellow or wither, so that the rooting rate of the semiliquidambar cathayensis tissue culture seedlings rooted outside the bottle can reach 90%, and the survival rate reaches 95%.
Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made in the above embodiments by those of ordinary skill in the art without departing from the principle and spirit of the present invention.
Claims (8)
1. A method for rooting tissue culture seedlings of semiliquidambar cathayensis outside a bottle is characterized by comprising the following steps:
rejuvenation of tissue culture seedlings: inoculating the tissue culture seedling of semiliquidambar cathayensis to an improved MS culture medium to induce and culture bud clusters to grow robustly, and obtaining a rejuvenated bottle seedling; the improved MS culture medium is as follows: NH (NH)4NO3500mg/L，KNO31900mg/L，MgSO4·7H2O 370mg/L，KH2PO4225mg/L，CaCl2·2H2O 440mg/L，MnSO4·4H2O 16.9mg/L，ZnSO4·7H2O 8.6mg/L，H3BO36.2mg/L，KI 0.83mg/L，Na2MoO4·2H2O 0.25mg/L，CuSO4·5H2O 0.025mg/L，CoCl2·6H2O0.025mg/L，FeSO4·7H2O 27.8mg/L，Na2-EDTA·2H2O37.3mg/L, glycine 2.0mg/L, thiamine hydrochloride 2.0mg/L, pyridoxine hydrochloride 0.5mg/L, IVB2.0mg/L of nicotinic acid and 100mg/L of inositol;
rooting outside the bottle: filling the sterilized seedling substrate into a rooting container, wherein the height of the seedling substrate is 2cm, trowelling and compacting the seedling substrate, thoroughly spraying clear water, poking a plurality of small holes with the depth of 1.0-1.5cm on the seedling substrate, selecting a rejuvenation bottle seedling with the height of 2-3 cm strong buds, cutting the strong buds, quickly dipping the strong buds with a rooting agent, inserting the strong buds into the small holes of the seedling substrate, and covering the rooting container; the seedling culture substrate is a mixture of peat, vermiculite and perlite; spreading peat, exposing the peat in the sun for 2-3 days for disinfection, bagging the disinfected peat for later use, mixing the disinfected peat, vermiculite and perlite according to a proportion before rooting and transplanting, uniformly mixing the peat, the vermiculite and the perlite to obtain a mixed matrix, disinfecting the mixed matrix by using a 0.3% potassium permanganate solution until the mixed matrix is wet, and closing the disinfected mixed matrix for at least one day to obtain a seedling culture matrix;
seedling stage management: transferring the rooting container with the seedlings inserted into the culture room to culture for 15 days, controlling the temperature at 26 +/-2 ℃, turning on light after dark culture for 2-3 days, and illuminating for 10 hours every day; moving to a seedling hardening shed on the 16 th day, and continuing to take root and harden the seedlings, wherein the temperature of the seedling hardening shed is 15-30 ℃, and the humidity is more than or equal to 70%; on day 16-18, from 10 am to 4 pm, the internal and external sunshades are completely unfolded; on the 19 th-22 th day, from 10 am to 4 pm, the sun is shaded and unfolded, then the seedlings are hardened in full light until semi-lignification, the leaves are thickened and become green, and the seedlings can be planted or transplanted in the field.
2. The method for ex-vitro rooting of the tissue culture seedlings of semiliquidambar cathayensis as claimed in claim 1, wherein the weight ratio of peat, vermiculite and perlite in the seedling substrate in the ex-vitro rooting step is 3:1: 1.
3. The method for rooting of semiliquidambar cathayensis tissue culture seedlings outside bottles as claimed in claim 1, wherein the rooting container in the step of rooting outside bottles is a plastic preservation box with a cover.
4. The method for rooting semiliquidambar cathayensis tissue culture seedlings outside the bottles according to claim 3, wherein the specification of the plastic preservation box is 20cm x 13cm x 12 cm.
5. The method for ex-vitro rooting of semiliquidambar cathayensis tissue culture seedlings according to claim 1, wherein the rooting agent in the ex-vitro rooting step is formed by mixing indolebutyric acid and naphthylacetic acid.
6. The method for rooting of semiliquidambar cathayensis tissue culture seedlings outside bottles according to claim 5, wherein the mass ratio of the indolebutyric acid to the naphthylacetic acid is 3: 2.
7. The method for ex-vitro rooting of semiliquidambar cathayensis tissue culture seedlings according to claim 1, wherein the concentration of the rooting agent in the ex-vitro rooting step is 800 ppm.
8. The method for rooting of semiliquidambar cathayensis tissue culture seedlings outside bottles according to claim 1, wherein the tissue culture seedlings are tissue culture seedlings of semiliquidambar cathayensis or tissue culture seedlings of semiliquidambar cathayensis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710769397.4A CN107494264B (en) | 2017-08-31 | 2017-08-31 | Method for rooting semiliquidambar cathayensis tissue culture seedling outside bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710769397.4A CN107494264B (en) | 2017-08-31 | 2017-08-31 | Method for rooting semiliquidambar cathayensis tissue culture seedling outside bottle |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107494264A CN107494264A (en) | 2017-12-22 |
| CN107494264B true CN107494264B (en) | 2020-01-14 |
Family
ID=60694580
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710769397.4A Active CN107494264B (en) | 2017-08-31 | 2017-08-31 | Method for rooting semiliquidambar cathayensis tissue culture seedling outside bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107494264B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113728845A (en) * | 2021-09-17 | 2021-12-03 | 贵州省林业科学研究院 | Method for rooting of Ardisia mamillata Hance tissue culture seedlings outside bottles |
| CN116849079A (en) * | 2023-08-30 | 2023-10-10 | 湖南省植物园 | Container seedling raising method for Semiliquidambar cathayensis |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104823857A (en) * | 2015-05-18 | 2015-08-12 | 江苏农林职业技术学院 | Seedling raising method for promoting ex-vitro rooting of blueberry tissue culture seedlings |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070149805A1 (en) * | 2005-12-23 | 2007-06-28 | Stephen F. Austin State University | Method for the extraction and purification of shikimic acid |
| CN102301958B (en) * | 2011-07-27 | 2013-05-08 | 地缘（厦门）生物科技有限公司 | Method for culturing in vitro tissues of Semiliquidambar cathayensis |
| CN102823497B (en) * | 2012-09-19 | 2015-01-14 | 广东省林业科学研究院 | Clonal tissue culture breeding method of Liquidambar formosana hance |
| CN103371093B (en) * | 2013-07-30 | 2015-05-27 | 杭州植物园 | Azalea tissue culture seedling out-bottle rooting method |
| CN105766633B (en) * | 2016-03-09 | 2018-06-01 | 广西壮族自治区林业科学研究院 | A kind of narrow leaved tea tree tissue culture subculture bud outside sprout-cultivating-bottle method |
| CN106171986B (en) * | 2016-07-12 | 2018-03-30 | 玉林师范学院 | A kind of Pterospermi Heterophylli seed sprouts the tissue culture and rapid propagation method of approach |
-
2017
- 2017-08-31 CN CN201710769397.4A patent/CN107494264B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104823857A (en) * | 2015-05-18 | 2015-08-12 | 江苏农林职业技术学院 | Seedling raising method for promoting ex-vitro rooting of blueberry tissue culture seedlings |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107494264A (en) | 2017-12-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102612961B (en) | Method for cultivating olive seedlings | |
| CN107493908A (en) | A kind of Damask Rose epicormic branch cutting method | |
| CN104756738A (en) | Cutting seedling method for twigs of elm of solar greenhouse | |
| CN109618932B (en) | Method for inducing adventitious buds of rhododendron dauricum and regenerating plants | |
| CN109258460A (en) | Micro-stem tip culture combines the breeding method of heat treatment acquisition Zengcheng honey chrysanthemum detoxic seedling | |
| CN103988762A (en) | Water culturing and transplanting method for apple tissue culture seedlings | |
| CN107162722A (en) | A kind of cultural method of epipremnum aureum | |
| CN103168692B (en) | Salix saposhnikovii tissue culture method | |
| CN107494264B (en) | Method for rooting semiliquidambar cathayensis tissue culture seedling outside bottle | |
| CN102144543A (en) | Tissue culture and rapid propagation technology for Clematis 'Arabella' | |
| CN103039366A (en) | Industrial production method of mycorrhizal seedlings of Changbai Mountain rhododendron plants | |
| CN105145363B (en) | It is a kind of to significantly improve the method that China fir tissue culture produces emergence rate | |
| CN110786240B (en) | Ex-vitro rooting method for callicarpa chinensis tissue culture seedlings | |
| CN113197091A (en) | Passion fruit tissue culture medium and application thereof in rapid propagation of detoxified Passion fruit tissue culture seedlings | |
| CN105028193A (en) | Breeding method for generating micro adventitious buds through induction of legacy leaves | |
| CN1799338A (en) | Tissue-culturing and seedling-growing method for rosa canina | |
| CN102119663A (en) | Tissue culture quick propagation technology of clematis mademe Julia correvon | |
| CN111133960A (en) | Transplanting method of clematis foraging tissue culture seedlings | |
| CN107667865B (en) | A kind of Ming River ceratostigma plumbaginoides Bunge subculture quick-breeding method | |
| CN110754360A (en) | Integrated solution for rooting, seedling exercising and domesticating of tissue culture seedlings | |
| CN107466850B (en) | Blueberry plantation and its seedling fast breeding method | |
| CN110999782A (en) | Tissue culture and rapid propagation method for gardenia jasminoides | |
| CN102301958A (en) | Method for culturing in vitro tissues of Semiliquidambar cathayensis | |
| CN108849510A (en) | Rooting method in clematis kind ' Avant-Garde ' tissue-cultured seedling bottle | |
| CN104956880B (en) | A kind of quick Taxus mandia propagating method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210427 Address after: 363000 no.10-2, fengle village, shiting Town, Xiangcheng District, Zhangzhou City, Fujian Province Patentee after: Zhangzhou geo Biotechnology Co.,Ltd. Address before: 128 room 800, Subtropical Plant Research Institute, No. Jiahe Road, Huli District, Fujian,, Xiamen Patentee before: Geo (Xiamen) Biotechnology Co.,Ltd. |