CN107490656A - Test device and its application method - Google Patents
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- CN107490656A CN107490656A CN201710624774.5A CN201710624774A CN107490656A CN 107490656 A CN107490656 A CN 107490656A CN 201710624774 A CN201710624774 A CN 201710624774A CN 107490656 A CN107490656 A CN 107490656A
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- G—PHYSICS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The present invention, which provides a kind of test device and its application method, device, to be included：Cosolvent quantitative filling unit (1), solvent holding vessel (2), solvent quantitative filling unit (3), automatic gradient equivalent drug solution preparing unit (4) step by step, laboratory sample mother liquor quantitative filling unit (5), the straight line electric cylinders sliding block (6) being arranged on straight line electric cylinders track (12), multiple number consecutively laboratory sample dilution mixed cylinders (7), multiple number consecutively laboratory sample storage cylinders (8), the multiple liquid feeding collection ports (10) being arranged in multiple laboratory sample dilution mixed cylinders (7) and end laboratory sample storage cylinder, multiple number consecutively aquatile experiment cylinder units (9) and the drainage collection groove (11) for being arranged at aquatile experiment cylinder unit (9) bottom；Wherein, laboratory sample dilution mixed cylinder (7), laboratory sample storage cylinder (8) are consistent with the number of aquatile experiment cylinder unit (9).The test device can realize the automatic dilution of laboratory sample, the automatic filling equivalent cycle renewal of laboratory sample solution, the flowing water test of large-scale aquatile breeding toxicity.
Description
Technical field
The present invention relates to technical field of chemistry, more particularly to a kind of test device and its application method.
Background technology
In chemical field, it usually needs by doing some tests for realizing the feature to biology, for example, by experiment cup
Middle test aquatile is (such as：, will also be to each, it is necessary to be put into the decoction of equivalent in each experiment cup during growth conditions Magna)
Decoction in experiment cup synchronizes equivalent renewal, while observes the growth conditions of each experiment water in the cup life biology.
Therefore, it is necessary to which a kind of continuous-flow type Daphnia magna breeding toxicity tester completes automatic gradient equivalent laboratory sample step by step
Prepare, the automatic dilution of laboratory sample, the automatic filling equivalent cycle renewal of laboratory sample solution, so as to realize that Daphnia magna is bred
The flowing water test of toxicity.
The content of the invention
The embodiment of the present invention provides a kind of test device and its application method, to solve to complete automatic gradient equivalent step by step
The preparation of concentration experiment sample, the automatic dilution of laboratory sample, the automatic filling equivalent cycle renewal of laboratory sample solution, so as to
The problem of realizing the flowing water test of Daphnia magna breeding toxicity.
The embodiment of the present invention provides a kind of test device, including：It is cosolvent quantitative filling unit 1, solvent holding vessel 2, molten
Drug solution preparing unit 4, laboratory sample mother liquor quantitative filling unit 5, the setting step by step of agent quantitative filling unit 3, automatic gradient equivalent
In the straight line electric cylinders sliding block 6 on straight line electric cylinders track 12, multiple number consecutively laboratory samples dilution mixed cylinders 7, multiple compile successively
Number laboratory sample storage cylinder 8, be arranged at it is more in multiple laboratory samples dilution mixed cylinders 7 and end laboratory sample storage cylinder
Individual liquid feeding collection port 10, multiple number consecutively aquatiles test cylinder unit 9 and are arranged at the aquatile experiment cylinder unit 9
The drainage collection groove 11 of bottom；Wherein, laboratory sample dilution mixed cylinder 7, the laboratory sample storage cylinder 8 and described aquatic
The number of Bioexperiment cylinder unit 9 is consistent.
In a possible embodiment, the cosolvent quantitative filling unit 1 and the multiple number consecutively laboratory sample
The liquid feeding collection port 10 of first laboratory sample dilution mixed cylinder 7 in dilution mixed cylinder 7 is turned on by flexible pipe, wherein, the reality
Sample dilution mixed cylinder 7 is tested to be provided with and correspond to liquid feeding collection port 10 therewith.
In a possible embodiment, the automatic gradient equivalent step by step determine by drug solution preparing unit 4 and laboratory sample mother liquor
Amount filling unit 5 and the end laboratory sample dilution mixed cylinder 7 in the multiple number consecutively laboratory sample dilution mixed cylinder 7
Liquid feeding collection port 10 turned on by flexible pipe.
In a possible embodiment, the solvent holding vessel 2 and the solvent quantitative filling unit 3 and the autostairs
Drug solution preparing unit 4 is turned on degree equivalent by flexible pipe step by step；The flexible pipe of the solvent quantitative filling unit 3 and the automatic gradient
Equivalent step by step with the straight line electric cylinders sliding block 6 fix by the flexible pipe of drug solution preparing unit 4, so that the solvent quantitative filling unit 3
Institute is flowed into by the slip of the straight line electric cylinders sliding block 6 with the decoction in the automatic gradient equivalent step by step drug solution preparing unit 4
State in laboratory sample dilution mixed cylinder 7.
In a possible embodiment, the multiple number consecutively laboratory sample dilutes the setting section in mixed cylinder 7
Bottom is provided with flexible pipe and hose valves 13 is provided with the flexible pipe, wherein, the setting section include except it is the multiple according to
The part at the end in secondary numbering laboratory sample dilution mixed cylinder 7；The bottom of the laboratory sample dilution mixed cylinder 7 is provided with
Magnetic stirring apparatus 14.
In a possible embodiment, the flexible pipe and reference numeral of the laboratory sample dilution mixed cylinder 7 are arranged at
The laboratory sample storage cylinder 8 turn on, and the experiment of the end in the multiple number consecutively laboratory sample storage cylinder 8
Sample storage cylinder 8 is provided with corresponding liquid feeding collection port 10.
In a possible embodiment, the bottom of the laboratory sample storage cylinder 8 is provided with flexible pipe and on the flexible pipe
Hose valves 13 are provided with, and the bottom of the laboratory sample storage cylinder 8 is provided with magnetic stirring apparatus 14.
In a possible embodiment, the flexible pipe of the laboratory sample storage cylinder 8 and the water of reference numeral are arranged at
Raw Bioexperiment cylinder unit 9 turns on.
In a possible embodiment, the bottom of the aquatile experiment cylinder unit 9 is provided with drainpipe, and the row
Water pipe turns on the drainage collection groove 11.
The embodiment of the present invention also provides a kind of application method of test device, including：Cosolvent quantitative filling unit will help
The first experiment sample that solvent mother liquor is injected in multiple number consecutively laboratory samples dilution mixed cylinders by flexible pipe dilutes mixing
In the liquid feeding collection port of cylinder；Laboratory sample mother liquor quantitative filling unit injects laboratory sample mother liquor by flexible pipe multiple to be compiled successively
End laboratory sample in number laboratory sample dilution mixed cylinder is diluted in the liquid feeding collection port of mixed cylinder；Solvent quantitative filling list
Member extracts solvent from solvent holding vessel and is implanted sequentially multiple number consecutively laboratory samples dilution mixed cylinders by the movement of straight line electric cylinders
In, while inject in the end laboratory sample storage cylinder in multiple number consecutively laboratory sample storage cylinders；Automatic gradient equivalent
Drug solution preparing unit extracts solvent from solvent holding vessel step by step, and diluted from multiple number consecutively laboratory samples in mixed cylinder
Extract in the laboratory sample dilution mixed cylinder of end and mixed by the mother liquor of one-level dilution；Lead to after mixed liquor is mixed step by step again
Crossing the movement of straight line electric cylinders, quantitatively to inject part laboratory sample dilution in multiple number consecutively laboratory samples dilution mixed cylinders successively mixed
Close in cylinder, the part Experiment sample dilution mixed cylinder includes：Removed in the multiple number consecutively laboratory sample dilution mixed cylinder
First and the laboratory sample dilution mixed cylinder at end；Part Experiment sample is diluted in mixed cylinder by the conducting of hose valves
Laboratory sample storage cylinder corresponding to dilution injection；By the conductings of hose valves by the test sample in the laboratory sample storage cylinder
Aquatile experiment cylinder unit corresponding to product injection；Aquatile tests cylinder unit and waste liquid is discharged into draining receipts by drainpipe
Collect groove.
Test device provided in an embodiment of the present invention, including：Cosolvent quantitative filling unit, solvent holding vessel, solvent are fixed
Amount filling unit, automatic gradient equivalent step by step drug solution preparing unit, laboratory sample mother liquor quantitative filling unit, be arranged at straight-line electric
Straight line electric cylinders sliding block on cylinder track, multiple number consecutively laboratory sample dilution mixed cylinders, the storage of multiple number consecutively laboratory samples
Deposit cylinder, multiple number consecutively aquatiles experiment cylinder unit and be arranged at multiple number consecutively aquatile experiment cylinder unit bottoms
Drainage collection groove；Wherein, the number of laboratory sample dilution mixed cylinder, laboratory sample storage cylinder and aquatile experiment cylinder unit 9
Unanimously.The test device can realize that the automatic of laboratory sample dilutes step by step, and the automatic filling equivalent cycle of laboratory sample solution is more
Newly, the test of continuous-flow type aquatile breeding toxicity.
Brief description of the drawings
Fig. 1 is a kind of structural representation of test device provided in an embodiment of the present invention；
Cosolvent quantitative filling unit -1, solvent holding vessel -2, solvent quantitative filling unit -3, automatic gradient equivalent are step by step
Drug solution preparing unit -4, laboratory sample mother liquor quantitative filling unit -5, straight line electric cylinders sliding block -6, laboratory sample dilution mixed cylinder -
7th, laboratory sample storage cylinder -8, aquatile experiment cylinder unit -9, the drainage collection groove -11 of liquid feeding collection port -10, straight line electric cylinders rail
Road -12, hose valves -13, magnetic stirring apparatus -14.
Embodiment
To make the purpose, technical scheme and advantage of the embodiment of the present invention clearer, below in conjunction with the embodiment of the present invention
In drawings and examples, the technical scheme in the embodiment of the present invention is explicitly described.
Fig. 1 is a kind of structural representation for test device that the present invention implements offer, as shown in figure 1, the test device, tool
Body includes：
Cosolvent quantitative filling unit 1, solvent holding vessel 2, solvent quantitative filling unit 3, automatic gradient equivalent medicine step by step
Liquid prepares unit 4, laboratory sample mother liquor quantitative filling unit 5, the straight line electric cylinders sliding block 6 being arranged on straight line electric cylinders track 12,
The multiple dilution of number consecutively laboratory sample mixed cylinder 7, multiple number consecutively laboratory sample storage cylinders 8, it is arranged at multiple experiment samples
Product dilute mixed cylinder 7 and multiple liquid feeding collection ports 10 in the laboratory sample storage cylinder of end, the aquatic life of multiple number consecutivelies
Thing tests cylinder unit 9 and is arranged at multiple number consecutively aquatile experiment bottom discharge collecting tanks 11 of cylinder unit 9；Wherein, it is real
It is consistent with the number of aquatile experiment cylinder unit 9 to test sample dilution mixed cylinder 7, laboratory sample storage cylinder 8.
In this embodiment, laboratory sample dilution mixed cylinder 7, laboratory sample storage cylinder 8 and aquatile test cylinder unit 9
Number is arranged to 8.
Alternatively, cosolvent quantitative filling unit 1 and first in multiple number consecutively laboratory samples dilution mixed cylinder 7
The liquid feeding collection port 10 of laboratory sample dilution mixed cylinder 7 is turned on by flexible pipe, wherein, laboratory sample dilution mixed cylinder 7 is provided with
Liquid feeding collection port 10 is corresponded to therewith.
Alternatively, automatic gradient equivalent step by step drug solution preparing unit 4 and laboratory sample mother liquor quantitative filling unit 5 with it is multiple
The liquid feeding collection port 10 of end laboratory sample dilution mixed cylinder 7 in number consecutively laboratory sample dilution mixed cylinder 7 is by soft
Pipe turns on.
Alternatively, solvent holding vessel 2 and solvent quantitative filling unit 3 and automatic gradient equivalent drug solution preparing unit 4 step by step
Turned on by flexible pipe；The flexible pipe of solvent quantitative filling unit 3 and automatic gradient equivalent step by step the flexible pipe of drug solution preparing unit 4 with it is straight
Line electric cylinders sliding block 6 is fixed, so that solvent quantitative filling unit 3 and automatic gradient the equivalent decoction in drug solution preparing unit 4 step by step
Laboratory sample is quantitatively flowed into by the slip of straight line electric cylinders sliding block 6 to dilute in mixed cylinder 7.
Alternatively, the bottom of the setting section in multiple number consecutively laboratory samples dilution mixed cylinders 7 be provided with flexible pipe and
Hose valves 13 are provided with flexible pipe, wherein, setting section is included except in multiple number consecutively laboratory samples dilution mixed cylinder 7
The part at end；The bottom of laboratory sample dilution mixed cylinder 7 is provided with magnetic stirring apparatus 14.
Alternatively, the laboratory sample storage cylinder 8 of the flexible pipe and reference numeral that are arranged at laboratory sample dilution mixed cylinder 7 is led
It is logical, and the end laboratory sample storage cylinder 8 in multiple number consecutively laboratory sample storage cylinders 8 is provided with corresponding liquid feeding
Collection port 10.
Alternatively, the bottom of laboratory sample storage cylinder 8 is provided with flexible pipe and hose valves 13, Yi Jishi is provided with flexible pipe
The bottom for testing sample storage cylinder 8 is provided with magnetic stirring apparatus 14.
Alternatively, the aquatile experiment cylinder unit 9 of the flexible pipe and reference numeral that are arranged at laboratory sample storage cylinder 8 is led
It is logical.
Alternatively, the bottom of aquatile experiment cylinder unit 9 is provided with drainpipe, and drainpipe is led with drainage collection groove 11
It is logical.
The application method of above-mentioned test device is specific as follows：
Cosolvent mother liquor is injected multiple number consecutively laboratory samples dilutions by cosolvent quantitative filling unit by flexible pipe to be mixed
In the liquid feeding collection port for closing the first experiment sample dilution mixed cylinder in cylinder；Laboratory sample mother liquor quantitative filling unit will test
The end laboratory sample dilution mixing that sample mother liquor is injected in multiple number consecutively laboratory samples dilution mixed cylinders by flexible pipe
In the liquid feeding collection port of cylinder；Solvent quantitative filling unit is multiple by the movement injection of straight line electric cylinders from solvent holding vessel extraction solvent
End laboratory sample dilution mixed cylinder in number consecutively laboratory sample dilution mixed cylinder, laboratory sample is formed after being sufficiently stirred
Mother liquor primary diluent；Drug solution preparing unit extracts solvent to automatic gradient equivalent from solvent holding vessel step by step, and from it is multiple according to
Extract to be entered by the mother liquor of one-level dilution in end laboratory sample dilution mixed cylinder in secondary numbering laboratory sample dilution mixed cylinder
Row mixing；Mixed liquor is diluted step by step by setup parameter again multiple number consecutivelies experiment samples are implanted sequentially by the movement of straight line electric cylinders
In product dilution mixed cylinder in part laboratory sample dilution mixed cylinder, part Experiment sample dilution mixed cylinder includes：It is multiple to compile successively
Except the laboratory sample dilution mixed cylinder at first and end in number laboratory sample dilution mixed cylinder；By the conductings of hose valves by portion
Divide laboratory sample storage cylinder corresponding to dilution injection in laboratory sample dilution mixed cylinder；Sample will be tested by the conducting of hose valves
Aquatile experiment cylinder unit corresponding to test specimen injection in product storage cylinder；Aquatile experiment cylinder unit passes through drainpipe
Waste liquid is discharged into drainage collection groove.
The number of cylinder unit 9 is tested with laboratory sample dilution mixed cylinder 7, laboratory sample storage cylinder 8 and aquatile below
It is arranged to 8 to be specifically introduced：
It should be noted that：The bottom of solvent quantitative filling unit is provided with solubilizer pipe, automatic gradient equivalent by
Chemical feed pipe is provided with level drug solution preparing unit.
Chemical feed pipe and solubilizer pipe are moved to by the laboratory sample that code name is 7-2 by the movement of straight line electric cylinders and dilute mixed cylinder
Liquid feeding collection port, according to setup parameter by automatic gradient equivalent step by step drug solution preparing unit first from code name be 7-8 experiment sample
Laboratory sample mother liquor one-level dilute solution is extracted in product dilution mixed cylinder, it is then further according to setup parameter that part Experiment sample is female
Liquid one-level dilute solution is quantitatively adding the laboratory sample that code name is 7-2 and dilutes mixed cylinder, while by solvent quantitative filling unit root
Mixed cylinder filling solvent is diluted to the laboratory sample that code name is 7-2 according to setup parameter is quantitative, it is dense to complete laboratory sample first gradient
Spend the preparation of dilute solution.Chemical feed pipe and solubilizer pipe are moved to the experiment sample that code name is 7-3 by next step straight line electric cylinders movement
Product dilute the liquid feeding collection port of mixed cylinder, and drug solution preparing unit stores up automatic gradient equivalent according to setup parameter is quantitative from solvent step by step
Deposit tank and extract solvent and complete laboratory sample and successively decrease the preparation of the second gradient concentration solutions preparatory.Second gradient solutions preparatory is prepared
Afterwards, further according to setup parameter, from automatic gradient equivalent, drug solution preparing unit dilutes mixing to the laboratory sample that code name is 7-3 step by step
Cylinder filling laboratory sample successively decreases the second gradient concentration solutions preparatory, while is quantified by solvent quantitative filling unit according to setup parameter
Mixed cylinder is diluted to the laboratory sample that code name is 7-3 and fills solvent, is completed laboratory sample gradient and is successively decreased the second gradient dilution solution
Preparation.Chemical feed pipe and solubilizer pipe are moved to the laboratory sample that code name is 7-4 and dilute mixing by next step straight line electric cylinders movement
The liquid feeding collection port of cylinder, drug solution preparing unit is quantitative molten from the extraction of solvent holding vessel according to setup parameter step by step for automatic gradient equivalent
Agent is completed laboratory sample and successively decreased the preparation of 3rd gradient concentration solutions preparatory.After 3rd gradient concentration solutions preparatory is prepared, then root
According to setup parameter, from automatic gradient equivalent, drug solution preparing unit dilutes mixed cylinder filling in fact to the laboratory sample that code name is 7-4 step by step
Test sample to successively decrease 3rd gradient concentration solutions preparatory, while quantitative according to setup parameter from solvent quantitative filling unit be to code name
7-4 laboratory sample dilution mixed cylinder filling solvent, complete laboratory sample gradient and successively decrease the preparation of 3rd gradient dilute solution.Under
The liquid feeding that chemical feed pipe and solubilizer pipe are moved to the laboratory sample dilution mixed cylinder that code name is 7-5 by one step straight line electric cylinders movement is received
Ji Kou, drug solution preparing unit extracts solvent completion experiment to automatic gradient equivalent according to setup parameter is quantitative from solvent holding vessel step by step
Sample successively decreases the preparation of 4th gradient concentration solutions preparatory.After 4th gradient concentration solutions preparatory is prepared, further according to setup parameter
From automatic gradient equivalent, drug solution preparing unit successively decreases to the laboratory sample dilution mixed cylinder filling laboratory sample that code name is 7-5 step by step
4th gradient concentration solutions preparatory, at the same it is quantitative to the experiment that code name is 7-5 according to setup parameter from solvent quantitative filling unit
Sample dilution mixed cylinder filling solvent, complete laboratory sample gradient and successively decrease the preparation of 4th gradient dilute solution.Next step straight line
Chemical feed pipe and solubilizer pipe are moved to the liquid feeding collection port for the laboratory sample dilution mixed cylinder that code name is 7-6 by electric cylinders movement, from
Dynamic gradient equivalent step by step according to setup parameter is quantitative from solvent holding vessel extraction solvent completion laboratory sample pass by drug solution preparing unit
Subtract the preparation of the 5th gradient concentration solutions preparatory.After 5th gradient concentration solutions preparatory is prepared, further according to setup parameter by automatic
Gradient equivalent step by step drug solution preparing unit to code name be 7-6 laboratory sample dilute mixed cylinder fill laboratory sample successively decrease the 5th ladder
Concentration solutions preparatory is spent, at the same it is quantitative dilute to the laboratory sample that code name is 7-6 according to setup parameter from solvent quantitative filling unit
Mixed cylinder filling solvent is released, laboratory sample gradient is completed and successively decreases the preparation of the 5th gradient dilution solution.Next step straight line electric cylinders are moved
The dynamic liquid feeding collection port that chemical feed pipe and solubilizer pipe are moved to the laboratory sample dilution mixed cylinder that code name is 7-7, automatic gradient
Equivalent step by step drug solution preparing unit according to setup parameter it is quantitative from solvent holding vessel extract solvent and complete laboratory sample successively decrease the 6th
The preparation of gradient concentration solutions preparatory.After 6th gradient experiment sample solution is prepared, further according to setup parameter by automatic gradient etc.
Drug solution preparing unit to the laboratory sample that code name is 7-7 dilutes mixed cylinder and fills laboratory sample and successively decreases the 6th gradient concentration amount step by step
Solutions preparatory, while dilute mixing to the laboratory sample that code name is 7-7 according to setup parameter is quantitative from solvent quantitative filling unit
Cylinder fills solvent, completes laboratory sample gradient and successively decreases the preparation of the 6th gradient dilution solution.Experiment is completed by above procedure
Sample mother liquor one-level dilute solution by the two level dilute solution of 6 constant gradients that successively decrease preparation.
Then solubilizer pipe is moved to the liquid feeding receipts for the laboratory sample dilution mixed cylinder that code name is 7-1 by the movement of straight line electric cylinders
Ji Kou, diluted according to setup parameter from solvent quantitative filling unit according to setup parameter is quantitative to the laboratory sample that code name is 7-1
Mixed cylinder fills solvent, is diluted from cosolvent quantitative filling unit according to setup parameter is quantitative to the laboratory sample that code name is 7-1
Mixed cylinder fills cosolvent, completes the preparation of the dilute solution of cosolvent.
The hose valves point of mixed cylinder outlet are diluted according to the laboratory sample that setup parameter time opening code name is 7-1 to 7-7
Solution is not supplemented to the laboratory sample storage cylinder that code name is 8-1 to 8-7, while solubilizer pipe is moved to by the movement of straight line electric cylinders
The laboratory sample storage cylinder that the liquid feeding collection port for the laboratory sample storage cylinder that code name is 8-8 is 8-8 to code name according to setup parameter
Quantitative filling solvent.
The hose valves exported according to the laboratory sample storage cylinder that setup parameter time opening code name is 8-1 to 8-8 respectively will
Solution supplements tests cylinder unit to the aquatile that code name is 9-1 to 9-8.The solution at different levels that are diluted step by step by gradient equivalent, help
Dilute solution, the solvent of solvent mother liquor, which complete, once to be updated.
By above-mentioned, mode can realize the automatic dilution of laboratory sample, the automatic filling equivalent cycle of laboratory sample solution
Renewal, the test of continuous-flow type aquatile breeding toxicity.
So far, although those skilled in the art will appreciate that detailed herein have shown and described multiple showing for the present invention
Example property embodiment, still, still can be direct according to present disclosure without departing from the spirit and scope of the present invention
It is determined that or derive many other variations or modifications for meeting the principle of the invention.Therefore, the scope of the present invention is understood that and recognized
It is set to and covers other all these variations or modifications.
Claims (10)
- A kind of 1. test device, it is characterised in that including：Cosolvent quantitative filling unit (1), solvent holding vessel (2), solvent are fixed Amount filling unit (3), automatic gradient equivalent step by step drug solution preparing unit (4), laboratory sample mother liquor quantitative filling unit (5), set The straight line electric cylinders sliding block (6) being placed on straight line electric cylinders track (12) is multiple number consecutively laboratory samples dilution mixed cylinders (7), more Individual number consecutively laboratory sample storage cylinder (8), multiple number consecutively aquatiles experiment cylinder unit (9), it is arranged at multiple experiments Sample dilution mixed cylinder (7) and multiple liquid feeding collection ports (10) in the laboratory sample storage cylinder of end and it is arranged at the water The drainage collection groove (11) of raw Bioexperiment cylinder unit (9) bottom；Wherein, the laboratory sample dilution mixed cylinder (7), the laboratory sample storage cylinder (8) and aquatile experiment cylinder The number of unit (9) is consistent.
- 2. device according to claim 1, it is characterised in that the cosolvent quantitative filling unit (1) with it is the multiple The liquid feeding collection port (10) of first experiment sample dilution mixed cylinder (7) in number consecutively laboratory sample dilution mixed cylinder (7) Turned on by flexible pipe, wherein, the laboratory sample dilution mixed cylinder (7) is provided with corresponds to liquid feeding collection port (10) therewith.
- 3. device according to claim 2, it is characterised in that automatic gradient equivalent drug solution preparing unit (4) step by step With the end in laboratory sample mother liquor quantitative filling unit (5) and the multiple number consecutively laboratory sample dilution mixed cylinder (7) The liquid feeding collection port (10) of individual laboratory sample dilution mixed cylinder (7) is turned on by flexible pipe.
- 4. device according to claim 1, it is characterised in that the solvent holding vessel (2) and the solvent quantitative filling Drug solution preparing unit (4) is turned on by flexible pipe step by step for unit (3) and the automatic gradient equivalent；The solvent quantitative filling unit (3) flexible pipe of drug solution preparing unit (4) and the straight line electric cylinders sliding block (6) are solid step by step for flexible pipe and the automatic gradient equivalent It is fixed, so that the solvent of the solvent quantitative filling unit (3) and the automatic gradient equivalent are step by step in drug solution preparing unit (4) Decoction flows into the laboratory sample dilution mixing successively by the slip of the straight line electric cylinders sliding block (6) by order set in advance In cylinder (7).
- 5. the device according to claim 3 or 4, it is characterised in that the multiple number consecutively laboratory sample dilution mixing The bottom of setting section in cylinder (7) is provided with flexible pipe and hose valves (13) is provided with the flexible pipe, wherein, the setting Part is included except the part at the end in the multiple number consecutively laboratory sample dilution mixed cylinder (7)；The bottom of the laboratory sample dilution mixed cylinder (7) is provided with magnetic stirring apparatus (14).
- 6. device according to claim 1 or 5, it is characterised in that be arranged at the laboratory sample dilution mixed cylinder (7) The laboratory sample storage cylinder (8) conducting of the flexible pipe and reference numeral, and the multiple number consecutively laboratory sample stores The end laboratory sample storage cylinder (8) in cylinder (8) is provided with corresponding liquid feeding collection port (10).
- 7. device according to claim 6, it is characterised in that the bottom of the laboratory sample storage cylinder (8) is provided with soft Pipe and it is provided with hose valves (13) on the flexible pipe, and the bottom of the laboratory sample storage cylinder (8) is provided with magnetic force and stirred Mix device (14).
- 8. device according to claim 7, it is characterised in that be arranged at the flexible pipe of the laboratory sample storage cylinder (8) with Aquatile experiment cylinder unit (9) conducting of reference numeral.
- 9. device according to claim 7, it is characterised in that the bottom of the aquatile experiment cylinder unit (9) is set There is drainpipe, and the drainpipe turns on the drainage collection groove (11).
- A kind of 10. application method of test device, it is characterised in that including：Cosolvent mother liquor is injected multiple number consecutively laboratory samples by flexible pipe and diluted by step 1, cosolvent quantitative filling unit In the liquid feeding collection port of first experiment sample dilution mixed cylinder in mixed cylinder；Laboratory sample mother liquor is quantitatively injected multiple number consecutivelies by flexible pipe by step 2, laboratory sample mother liquor quantitative filling unit In the liquid feeding collection port of end laboratory sample dilution mixed cylinder in laboratory sample dilution mixed cylinder, solvent quantitative filling unit The end laboratory sample dilution that solvent is quantitatively injected in multiple number consecutively laboratory sample dilution mixed cylinders by flexible pipe is mixed In the liquid feeding collection port for closing cylinder, the primary diluent to form mother liquor is sufficiently stirred；Step 3, according to the automatic gradient equivalent of setup parameter, drug solution preparing unit extracts the primary diluent of mother liquor step by step, then incites somebody to action Partial mother liquid primary diluent quantitatively injects second laboratory sample dilution mixed cylinder, and solvent quantitative filling unit quantifies solvent Second laboratory sample dilution mixed cylinder of injection, is sufficiently stirred to form first gradient concentration dilution solution；Step 4, automatic gradient equivalent step by step drug solution preparing unit quantitatively extract solvent formed first gradient concentration dilution solution arrive The intermediate density solution of second gradient concentration dilute solution, then section transitions strength solution is quantitatively injected into the 3rd laboratory sample Mixed cylinder is diluted, while solvent is quantified the 3rd laboratory sample dilution mixed cylinder of injection by solvent quantitative filling unit, fully Stirring forms the second gradient concentration dilute solution；Step 4 is repeated several times, is formed in multiple laboratory samples dilution mixed cylinder except end laboratory sample dilution mixed cylinder Multiple gradient concentration dilute solutions that concentration is successively decreased successively；Part Experiment sample is diluted by the conducting of hose valves and tested corresponding to the dilution being sufficiently stirred in mixed cylinder injection Sample storage cylinder；By the conductings of hose valves by aquatic life corresponding to the test specimen being sufficiently stirred in laboratory sample storage cylinder injection Thing tests cylinder unit；Waste liquid is discharged into drainage collection groove by aquatile experiment cylinder unit by drainpipe.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111505324A (en) * | 2020-05-13 | 2020-08-07 | 安图实验仪器(郑州)有限公司 | Automatic solution diluting method |
| CN114051957A (en) * | 2021-11-15 | 2022-02-18 | 江苏雅信昆成检测科技有限公司 | Large-scale daphnia of continuous-flow type test device |
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| JPH06154669A (en) * | 1992-11-17 | 1994-06-03 | Sankyo Co Ltd | Automatic liquid medicine preparing device |
| JPH116788A (en) * | 1997-06-17 | 1999-01-12 | Yokogawa Analytical Syst Kk | Method and device for automatic preparation of solution |
| CN101023361A (en) * | 2004-07-22 | 2007-08-22 | 安姆根有限公司 | Method and apparatus for forming a dilution by fluid dispersion |
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| CN103072931A (en) * | 2012-12-26 | 2013-05-01 | 陆跃明 | Solution equivalent allocation device |
| CN205865681U (en) * | 2016-07-13 | 2017-01-11 | 环境保护部南京环境科学研究所 | Small -size aquatic organisms's continuous -flow type exposes device |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111505324A (en) * | 2020-05-13 | 2020-08-07 | 安图实验仪器(郑州)有限公司 | Automatic solution diluting method |
| CN114051957A (en) * | 2021-11-15 | 2022-02-18 | 江苏雅信昆成检测科技有限公司 | Large-scale daphnia of continuous-flow type test device |
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