CN107485626A - A kind of probiotics medicine for treating diarrhoea and preparation method thereof - Google Patents

A kind of probiotics medicine for treating diarrhoea and preparation method thereof Download PDF

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CN107485626A
CN107485626A CN201710701595.7A CN201710701595A CN107485626A CN 107485626 A CN107485626 A CN 107485626A CN 201710701595 A CN201710701595 A CN 201710701595A CN 107485626 A CN107485626 A CN 107485626A
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bacterium powder
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王小伟
赵景阳
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Shanxi Ecross Hongtai Biotechnology Co Ltd
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract

The invention belongs to drug field, and in particular to a kind of probiotics medicine for treating diarrhoea and preparation method thereof, the medicine includes the raw material of following weight proportion：10 40 parts of clostridium butyricum bacterium powder, 10 40 parts of lactobacillus acidophilus bacterium powder, 10 40 parts of bifidobacterium lactis bacterium powder, 16 parts of vitamin C, 24 34 parts of FOS, 10 40 parts of skimmed milk power, 27 parts of maltodextrin, 27 parts of lactose, 5 15 parts of inulin, by probiotics bacterium powder and vitamin C, FOS and skimmed milk power, maltodextrin, lactose and inulin formulated in combination, prescription is rigorous, compatibility science, it is evident in efficacy, energy both symptoms and root causes, treat rapidly and fundamentally diarrhoea.

Description

A kind of probiotics medicine for treating diarrhoea and preparation method thereof
Technical field
The invention belongs to drug field, and in particular to a kind of probiotics medicine for treating diarrhoea and preparation method thereof.
Background technology
At present, diarrhoea is a kind of common sympton, refers to defecation frequency significantly more than the frequency being accustomed on ordinary days, and excrement matter is thin, Moisture increase, daily feces volume is more than 200g, or containing not digesting food or purulence blood, mucus.Diarrhoea is often accompanied by defecation urgency sense, anus The symptoms such as door discomfort, incontinence.Diarrhoea is divided to acute and chronic two class.Acute diarrhea is fallen ill drastically, and the course of disease is within 2~3 weeks.It is chronic Diarrhoea refers to the recurrent diarrhoea that the course of disease is more than two months or the intermittent phase is in 2~4 weeks.At present, primary treatment medicine is mainly Antibiosis anti-inflammatory drug, antidiarrheal agent, Chinese medicine, probiotics etc., but curative effect is respectively weak, and doctor trained in Western medicine can not treat both principal and secondary aspect of disease, and side effect is bright Aobvious, the traditional Chinese medical science course for the treatment of is longer, is extremely difficult to good result in a short time.Accordingly, it is capable to mitigate slight illness rapidly and fundamentally cure diarrhoea And the medicine being free from side effects is clinical great difficult problem in the urgent need to address.
The content of the invention
In order to solve this problem, the present invention provides a kind of probiotics medicine for treating diarrhoea and preparation method thereof, by benefit Raw bacterium bacterium powder and vitamin C, FOS and skimmed milk power, maltodextrin, lactose and inulin formulated in combination, prescription is rigorous, matches somebody with somebody 5 science, evident in efficacy, energy both symptoms and root causes, treat rapidly and fundamentally diarrhoea.
Technical scheme is used by order to solve its technical problem：
A kind of probiotics medicine for treating diarrhoea, include the raw material of following weight proportion：Clostridium butyricum（Also known as Miyarisan, butyric acid Clostridium）Bacterium powder 10-40 parts, lactobacillus acidophilus bacterium powder 10-40 parts, bifidobacterium lactis bacterium powder 10-40 parts, vitamin C1-6 parts, FOS 24-34 parts, skimmed milk power 10-40 parts, maltodextrin 2-7 parts, lactose 2-7 parts, inulin 5-15 parts, always Viable count is 1 × 107More than cfu/g.
Above-mentioned probiotics medicine is oral formulations.
The formulation of above-mentioned probiotics medicine is capsule or powder preparation.
The preparation method of the probiotics medicine of above-mentioned treatment diarrhoea, comprises the following steps：
A, strain is selected：Choose clostridium butyricum strain；
B, the production of hybrid seeds：Sterile environment is selected, strain transfer needle is taken out and is put into 500 milliliters of triangular flasks equipped with culture medium, Then seal bottleneck to be put into incubator, 37 DEG C of the temperature of incubator, static gas wave refrigerator 12 hours；
C, sterilize：100 liters of fermentation tanks are subjected to empty elimination bacterium, culture medium is then placed in and is sterilized, 121 degrees Celsius of sterilising temp More than, pressure inside the tank 0.12MPa, continue 20min sterilizings and terminate, temperature in tank is then down to 37 DEG C with cooling water, tank internal pressure Power is down to 0.01MPa；
D, it is inoculated with：The seed in incubator is put into desinfection chamber first and is sampled microscopy, growth selection it is good be inoculated with, Turn on inoculation mouth closure, toward 100 liters of tanks in be passed through micro filtrated air, ensure that pressure inside the tank will not be zero, with inoculation flame Circle is placed on inoculation mouth, after lighting flame ring, opens inoculation lid completely, then triangular flask is placed on above flame ring, open Triangular flask, seed is poured into tank, inoculation lid is tightened after terminating, filtrated air is led into tank, pressure inside the tank is stopped to 0.06MPa Only, pressurize is carried out, temperature is kept for 37 DEG C, the MPa of pressure 0.06, and speed of agitator is adjusted to 60r/min, cultivates 12 hours；
E, culture transferring：Step b, the pipeline that the strain in 100 liters of fermentation tank then is passed through into sterilizing are carried out to 1000 liters of fermentation tanks Moving in 1000 liters of fermentation tanks for preparing culture medium, temperature is kept for 37 DEG C, and the MPa of pressure 0.06, speed of agitator is adjusted to 60r/min, Cultivate 12 hours, side viable count is sampled after growth well in 1000 liters of fermentation tanks；
F, centrifuge：Bacterium solution is turned clockwise by centrifuge, centrifuge speed for 16000r/min, carries out bacterium solution separation, is completed After take out bacterium mud；
G, freeze：Bacterium mud is placed in the flaggy of freeze dryer, carries out lyophilized operation, -45 DEG C of freeze dryer rear lane temperature is taken out Vacuum；
H, crush：Bacterium powder is obtained after crushing；
I, lactobacillus acidophilus bacterium powder and bifidobacterium lactis bacterium powder processed：By lactobacillus acidophilus, bifidobacterium lactis repeat step b to step Rapid h, obtain lactobacillus acidophilus bacterium powder and bifidobacterium lactis bacterium powder；
J, manufactured goods：By clostridium butyricum bacterium powder, lactobacillus acidophilus bacterium powder, bifidobacterium lactis bacterium powder and vitamin C, FOS, Skimmed milk power, maltodextrin, lactose and inulin are matched according to the drug weight and configured, and capsule is made after well mixed or is made Powder packs.
The effect of the various probiotics of the present invention is as follows：
Clostridium butyricum：The short chain fatty acids such as butyric acid can be produced, are the main battalion of the histiocytic regeneration of gut epithelium and reparation Material is supported, repairs impaired intestinal mucosa, and promotes the production of beneficial bacterium in enteron aisle, suppresses beneficial bacterium breeding；
Lactobacillus acidophilus：One of people and animal intestinal tract normal flora, suppress harmful bacteria growing, promote intestinal beneficial bacteria growing, adjust Whole disorderly intestinal microecology system；
Bifidobacterium lactis：Lactic acid and acetic acid are produced, has the function that to adjust gut flora, has fostered the ancestral home bacterium in enteron aisle, made Body colonization resistance raises, and is advantageous to the field planting of antagonism pathogenic bacteria and conditioned pathogen, has good work to treatment chronic diarrhea With.
The beneficial effects of the invention are as follows：The present invention compared with the existing simple Chinese medicine of in the market, Western medicine and probiotics, Both enteron aisle, which can have been nursed one's health, inherently improves human body constitution, can adjust the intestinal microecology system of disorder rapidly again, directly repair Impaired intestinal mucosa again, intestinal immunity is improved, is treated both principal and secondary aspect of disease.The effect of fundamentally playing prevention and treatment diarrhoea.
Embodiment
Embodiment 1：
A kind of probiotics medicine for treating diarrhoea, include the raw material of following weight proportion, 10 parts of clostridium butyricum bacterium powder, acidophilus breast bar 10 parts of bacterium bacterium powder, 10 parts of bifidobacterium lactis bacterium powder, 1 part of vitamin C, 24 parts of FOS, 10 parts of skimmed milk power, maltodextrin 2 Part, 2 parts of lactose, 5 parts of inulin, total viable count is 2 × 107cfu/g。
The preparation method of the probiotics medicine of above-mentioned treatment diarrhoea, comprises the following steps：
A, strain is selected：Choose clostridium butyricum strain；
B, the production of hybrid seeds：Sterile environment is selected, strain transfer needle is taken out and is put into 500 milliliters of triangular flasks equipped with culture medium, Then seal bottleneck to be put into incubator, 37 DEG C of the temperature of incubator, static gas wave refrigerator 12 hours；
C, sterilize：100 liters of fermentation tanks are subjected to empty elimination bacterium, culture medium is then placed in and is sterilized, sterilising temp 121 is Celsius More than degree, pressure inside the tank 0.12MPa, continue 20min sterilizings and terminate, temperature in tank is then down to 37 DEG C, in tank with cooling water Pressure is down to 0.01MPa；
D, it is inoculated with：The seed in incubator is put into desinfection chamber first and is sampled microscopy, growth selection it is good be inoculated with, Turn on inoculation mouth closure, toward 100 liters of tanks in be passed through micro filtrated air, ensure that pressure inside the tank will not be zero, with inoculation flame Circle is placed on inoculation mouth, after lighting flame ring, opens inoculation lid completely, then triangular flask is placed on above flame ring, open Triangular flask, seed is poured into tank, inoculation lid is tightened after terminating, filtrated air is led into tank, pressure inside the tank is stopped to 0.06MPa Only, pressurize is carried out, temperature is kept for 37 DEG C, the MPa of pressure 0.06, and speed of agitator is adjusted to 60r/min, cultivates 12 hours；
E, culture transferring：Step b, the pipeline that the strain in 100 liters of fermentation tank then is passed through into sterilizing are carried out to 1000 liters of fermentation tanks Moving in 1000 liters of fermentation tanks for preparing culture medium, temperature is kept for 37 DEG C, and the MPa of pressure 0.06, speed of agitator is adjusted to 60r/min, Cultivate 12 hours, side viable count is sampled after growth well in 1000 liters of fermentation tanks；
F, centrifuge：Bacterium solution is turned clockwise by centrifuge, centrifuge speed for 16000r/min, carries out bacterium solution separation, is completed After take out bacterium mud；
G, freeze：Bacterium mud is placed in the flaggy of freeze dryer, carries out lyophilized operation, -45 DEG C of freeze dryer rear lane temperature is taken out Vacuum；
H, crush：Bacterium powder is obtained after crushing；
I, lactobacillus acidophilus bacterium powder and bifidobacterium lactis bacterium powder processed：By lactobacillus acidophilus, bifidobacterium lactis repeat step b to step Rapid h, obtain lactobacillus acidophilus bacterium powder and bifidobacterium lactis bacterium powder；
J, manufactured goods：By clostridium butyricum bacterium powder, lactobacillus acidophilus bacterium powder, bifidobacterium lactis bacterium powder and vitamin C, FOS, Skimmed milk power, maltodextrin, lactose and inulin are matched according to the drug weight and configured, and capsule is made after well mixed.
Embodiment 2：
A kind of probiotics medicine for treating diarrhoea, include the raw material of following weight proportion, 40 parts of clostridium butyricum bacterium powder, acidophilus breast bar 40 parts of bacterium bacterium powder, 40 parts of bifidobacterium lactis bacterium powder, 6 parts of vitamin C, 34 parts of FOS, 40 parts of skimmed milk power, maltodextrin 7 Part, 7 parts of lactose, 15 parts of inulin, total viable count is 5 × 107cfu/g。
The preparation method of the probiotics medicine of above-mentioned treatment diarrhoea, comprises the following steps：
A, strain is selected：Choose clostridium butyricum strain；
B, the production of hybrid seeds：Sterile environment is selected, strain transfer needle is taken out and is put into 500 milliliters of triangular flasks equipped with culture medium, Then seal bottleneck to be put into incubator, 37 DEG C of the temperature of incubator, static gas wave refrigerator 12 hours；
C, sterilize：100 liters of fermentation tanks are subjected to empty elimination bacterium, culture medium is then placed in and is sterilized, 121 degrees Celsius of sterilising temp More than, pressure inside the tank 0.12MPa, continue 20min sterilizings and terminate, temperature in tank is then down to 37 DEG C with cooling water, tank internal pressure Power is down to 0.01MPa；
D, it is inoculated with：The seed in incubator is put into desinfection chamber first and is sampled microscopy, growth selection it is good be inoculated with, Turn on inoculation mouth closure, toward 100 liters of tanks in be passed through micro filtrated air, ensure that pressure inside the tank will not be zero, with inoculation flame Circle is placed on inoculation mouth, after lighting flame ring, opens inoculation lid completely, then triangular flask is placed on above flame ring, open Triangular flask, seed is poured into tank, inoculation lid is tightened after terminating, filtrated air is led into tank, pressure inside the tank is stopped to 0.06MPa Only, pressurize is carried out, temperature is kept for 37 DEG C, the MPa of pressure 0.06, and speed of agitator is adjusted to 60r/min, cultivates 12 hours；
E, culture transferring：Step b, the pipeline that the strain in 100 liters of fermentation tank then is passed through into sterilizing are carried out to 1000 liters of fermentation tanks Moving in 1000 liters of fermentation tanks for preparing culture medium, temperature is kept for 37 DEG C, and the MPa of pressure 0.06, speed of agitator is adjusted to 60r/min, Cultivate 12 hours, side viable count is sampled after growth well in 1000 liters of fermentation tanks；
F, centrifuge：Bacterium solution is turned clockwise by centrifuge, centrifuge speed for 16000r/min, carries out bacterium solution separation, is completed After take out bacterium mud；
G, freeze：Bacterium mud is placed in the flaggy of freeze dryer, carries out lyophilized operation, -45 DEG C of freeze dryer rear lane temperature is taken out Vacuum；
H, crush：Probiotics bacterium powder is obtained after crushing；
I, lactobacillus acidophilus bacterium powder and bifidobacterium lactis bacterium powder processed：By lactobacillus acidophilus, bifidobacterium lactis repeat step b to step Rapid h, obtain lactobacillus acidophilus bacterium powder and bifidobacterium lactis bacterium powder；
J, manufactured goods：By clostridium butyricum bacterium powder, lactobacillus acidophilus bacterium powder, bifidobacterium lactis bacterium powder and vitamin C, FOS, Skimmed milk power, maltodextrin, lactose and inulin are matched according to the drug weight and configured, and powder pack is made after well mixed i.e. Can.
Embodiment 3：
A kind of probiotics medicine for treating diarrhoea, include the raw material of following weight proportion, 25 parts of clostridium butyricum bacterium powder, acidophilus breast bar 25 parts of bacterium bacterium powder, 25 parts of bifidobacterium lactis bacterium powder, 3 parts of vitamin C, 29 parts of FOS, 25 parts of skimmed milk power, maltodextrin 4 Part, 4 parts of lactose, 10 parts of inulin, total viable count is 4 × 107cfu/g。
The preparation method of the probiotics medicine of above-mentioned treatment diarrhoea, comprises the following steps：
A, strain is selected：Choose clostridium butyricum strain；
B, the production of hybrid seeds：Sterile environment is selected, strain transfer needle is taken out and is put into 500 milliliters of triangular flasks equipped with culture medium, Then seal bottleneck to be put into incubator, 37 DEG C of the temperature of incubator, static gas wave refrigerator 12 hours；
C, sterilize：100 liters of fermentation tanks are subjected to empty elimination bacterium, culture medium is then placed in and is sterilized, 121 degrees Celsius of sterilising temp More than, pressure inside the tank 0.12MPa, continue 20min sterilizings and terminate, temperature in tank is then down to 37 DEG C with cooling water, tank internal pressure Power is down to 0.01MPa；
D, it is inoculated with：The seed in incubator is put into desinfection chamber first and is sampled microscopy, growth selection it is good be inoculated with, Turn on inoculation mouth closure, toward 100 liters of tanks in be passed through micro filtrated air, ensure that pressure inside the tank will not be zero, with inoculation flame Circle is placed on inoculation mouth, after lighting flame ring, opens inoculation lid completely, then triangular flask is placed on above flame ring, open Triangular flask, seed is poured into tank, inoculation lid is tightened after terminating, filtrated air is led into tank, pressure inside the tank is stopped to 0.06MPa Only, pressurize is carried out, temperature is kept for 37 DEG C, the MPa of pressure 0.06, and speed of agitator is adjusted to 60r/min, cultivates 12 hours；
E, culture transferring：Step b, the pipeline that the strain in 100 liters of fermentation tank then is passed through into sterilizing are carried out to 1000 liters of fermentation tanks Moving in 1000 liters of fermentation tanks for preparing culture medium, temperature is kept for 37 DEG C, and the MPa of pressure 0.06, speed of agitator is adjusted to 60r/min, Cultivate 12 hours, side viable count is sampled after growth well in 1000 liters of fermentation tanks；
F, centrifuge：Bacterium solution is turned clockwise by centrifuge, centrifuge speed for 16000r/min, carries out bacterium solution separation, is completed After take out bacterium mud；
G, freeze：Bacterium mud is placed in the flaggy of freeze dryer, carries out lyophilized operation, -45 DEG C of freeze dryer rear lane temperature is taken out Vacuum；
H, crush：Probiotics bacterium powder is obtained after crushing；
I, lactobacillus acidophilus bacterium powder and bifidobacterium lactis bacterium powder processed：By lactobacillus acidophilus, bifidobacterium lactis repeat step b to step Rapid h, obtain lactobacillus acidophilus bacterium powder and bifidobacterium lactis bacterium powder；
J, manufactured goods：By clostridium butyricum bacterium powder, lactobacillus acidophilus bacterium powder, bifidobacterium lactis bacterium powder and vitamin C, FOS, Skimmed milk power, maltodextrin, lactose and inulin are matched according to the drug weight and configured, and capsule is made after well mixed.
Clinical case：
1st, object：Cao Yuying, sex：Female, age：68 years old；Cause of disease symptom：Suffered from diarrhoea incessantly after eating frost watermelon, instructions of taking：Often It twice, once two grams, treatment results：Symptom is obviously improved after taking capsule-type made from the present embodiment 3 once, is taken twice Symptom of diarrhea disappears afterwards；
2nd, object：Wen Shuai, sex：Man, age：28 years old；Cause of disease symptom：Suffered from diarrhoea incessantly after drinking ice beer, instructions of taking：Often It is secondary twice, once two grams, treatment results：Symptom of diarrhea disappears after taking powderous preparations made from the present embodiment 2 once；
3rd, object：Zhang Shuilong, sex：Man, age：42 years old；Cause of disease symptom：Suffered from diarrhoea incessantly after eating unhygienic food, the side of taking Method：Every time twice, once two grams, treatment results：Symptom is obviously improved after taking capsule made from the present embodiment 1 once, is taken Symptom of diarrhea disappears after twice.

Claims (2)

1. a kind of probiotics medicine for treating diarrhoea, it is characterised in that include the raw material of following weight proportion：Clostridium butyricum bacterium powder 10-40 parts, lactobacillus acidophilus bacterium powder 10-40 parts, bifidobacterium lactis bacterium powder 10-40 parts, vitamin C 1-6 parts, FOS 24- 34 parts, skimmed milk power 10-40 parts, maltodextrin 2-7 parts, lactose 2-7 parts, inulin 5-15 parts, total viable count is 1 × 107cfu/g More than.
2. a kind of preparation method for the probiotics medicine for treating diarrhoea, it is characterised in that comprise the following steps：
A, strain is selected：Choose clostridium butyricum strain；
B, the production of hybrid seeds：Sterile environment is selected, strain transfer needle is taken out and is put into 500 milliliters of triangular flasks equipped with culture medium, Then seal bottleneck to be put into incubator, 37 DEG C of the temperature of incubator, static gas wave refrigerator 12 hours；
C, sterilize：100 liters of fermentation tanks are subjected to empty elimination bacterium, culture medium is then placed in and is sterilized, 121 degrees Celsius of sterilising temp More than, pressure inside the tank 0.12MPa, continue 20min sterilizings and terminate, temperature in tank is then down to 37 DEG C with cooling water, tank internal pressure Power is down to 0.01MPa；
D, it is inoculated with：The seed in incubator is put into desinfection chamber first and is sampled microscopy, is inoculated with, turns on inoculation mouth sealing Lid, toward 100 liters of tanks in be passed through micro filtrated air, guarantee pressure inside the tank will not be zero, and inoculation mouth is placed on inoculation flame ring, After lighting flame ring, inoculation lid is opened completely, then triangular flask is placed on above flame ring, triangular flask is opened, seed is fallen Enter in tank, tighten inoculation lid after terminating, filtrated air is led into tank, pressure inside the tank to 0.06MPa stops, and carries out pressurize, temperature Degree is kept for 37 DEG C, the MPa of pressure 0.06, and speed of agitator is adjusted to 60r/min, is cultivated 12 hours；
E, culture transferring：Step b, the pipeline that the strain in 100 liters of fermentation tank then is passed through into sterilizing are carried out to 1000 liters of fermentation tanks Moving in 1000 liters of fermentation tanks for preparing culture medium, temperature is kept for 37 DEG C, and the MPa of pressure 0.06, speed of agitator is adjusted to 60r/min, Cultivate 12 hours, side viable count is sampled after growth well in 1000 liters of fermentation tanks；
F, centrifuge：Bacterium solution is turned clockwise by centrifuge, centrifuge speed for 16000r/min, carries out bacterium solution separation, is completed After take out bacterium mud；
G, freeze：Bacterium mud is placed in the flaggy of freeze dryer, carries out lyophilized operation, -45 DEG C of freeze dryer rear lane temperature is taken out Vacuum；
H, crush：After obtain bacterium powder；
I, lactobacillus acidophilus bacterium powder and bifidobacterium lactis bacterium powder processed：By lactobacillus acidophilus, bifidobacterium lactis repeat step b to step H, obtain lactobacillus acidophilus bacterium powder and bifidobacterium lactis bacterium powder；
J, manufactured goods：By clostridium butyricum bacterium powder, lactobacillus acidophilus bacterium powder, bifidobacterium lactis bacterium powder and vitamin C, FOS, Skimmed milk power, maltodextrin, lactose and inulin are matched according to the drug weight and configured, and capsule is made after well mixed or is made Powder packs.
CN201710701595.7A 2017-08-16 2017-08-16 A kind of probiotics medicine for treating diarrhoea and preparation method thereof Withdrawn CN107485626A (en)

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* Cited by examiner, † Cited by third party
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CN108888726A (en) * 2018-08-09 2018-11-27 深圳市博奥生物科技有限公司 A kind of preparation method and compound probiotic capsule of compound probiotic capsule
CN109161494A (en) * 2018-08-09 2019-01-08 北京市理化分析测试中心 With the composite bacteria agent and its preparation method and application for adjusting immune function
CN112618579A (en) * 2020-12-24 2021-04-09 南京农业大学 Compound probiotic preparation for preventing and treating piglet diarrhea as well as preparation method and application thereof

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108888726A (en) * 2018-08-09 2018-11-27 深圳市博奥生物科技有限公司 A kind of preparation method and compound probiotic capsule of compound probiotic capsule
CN109161494A (en) * 2018-08-09 2019-01-08 北京市理化分析测试中心 With the composite bacteria agent and its preparation method and application for adjusting immune function
CN112618579A (en) * 2020-12-24 2021-04-09 南京农业大学 Compound probiotic preparation for preventing and treating piglet diarrhea as well as preparation method and application thereof
CN112618579B (en) * 2020-12-24 2022-02-15 南京农业大学 Compound probiotic preparation for preventing and treating piglet diarrhea as well as preparation method and application thereof

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Application publication date: 20171219