CN107478852A - Antibiotics induction type hemolytic anemia micro-column gel agglutination assay detection kit - Google Patents
Antibiotics induction type hemolytic anemia micro-column gel agglutination assay detection kit Download PDFInfo
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- CN107478852A CN107478852A CN201710769984.3A CN201710769984A CN107478852A CN 107478852 A CN107478852 A CN 107478852A CN 201710769984 A CN201710769984 A CN 201710769984A CN 107478852 A CN107478852 A CN 107478852A
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- micro
- hemolytic anemia
- detection kit
- induction type
- box body
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
- G01N33/559—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody through a gel, e.g. Ouchterlony technique
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B13/00—Control arrangements specially designed for centrifuges; Programme control of centrifuges
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B15/00—Other accessories for centrifuges
- B04B15/08—Other accessories for centrifuges for ventilating or producing a vacuum in the centrifuge
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
Abstract
The invention discloses antibiotics induction type hemolytic anemia micro-column gel agglutination assay detection kit, Centrifugal Machine Control knob including box body and on the downside of box body, also include centrifugation cabin and minipump, motor and centrifugal shaft are installed inside the centrifugation cabin, the centrifugal shaft lower end is connected with motor, the centrifugal shaft upper end is provided with connecting rod, the connecting rod is connected with rack, the minipump is arranged on the inner right side bottom position of box body, the right upper portion of the box body is also equipped with three Reagent packaging containers, the Reagent packaging container is respectively connected with airway tube, magnetic valve is installed on the airway tube, the magnetic valve is electrically connected with electromagnetic valve switch, the air guide pipe end is connected on minipump, tray interior is provided with cooler.The present invention can provide detection reagent the environment that vacuum preserves, and can provide centrifugal treating service, simple in construction, novel in design, be worth promoting.
Description
Technical field
The invention belongs to technical field of medical detection, more particularly to a kind of antibiotics induction type hemolytic anemia
Micro-column gel agglutination assay detection kit.
Background technology
Biomedical detection technique is the important means and experimental tool of modern life science and medical development, with biological electricity
Pole and sensing technology are technical foundation, and a variety of subjects such as automation, electronics, computer, machinery, optics, multiple technologies are merged
It is integrated and integrated use is in biomedical sector, is concrete application of the detection technique in biomedical sector.Biomedicine inspection
The method of survey technology application engineering goes to measure the physiological parameter of the form of organism, physiological function and other state changes, such as blood
Pressure, body temperature, breathing, heart sound, CBF and physiology electric etc., it is in many necks such as clinical practice, sports medical science, physiological medical science researchs
Played a very important role in domain.
Clinical laboratory technology gradually changes traditional method of inspection, and new inspection technology carries for the diagnostic analysis of disease
The design of more quick, more accurate method Clinical Laboratory Instruments has been supplied more to focus on hommization, low cost and beneficial to ring
Protect.At present, global medical laboratory instrument product is technically positive develops towards digitlization, networking, miniaturization direction.It is proposed
The developing direction of laboratory instrument and equipment automates, is intelligent one by one, standardization, personalized and miniature portable.
Anaemia (anemia) refers to that human peripheral blood erythrocyte volume is reduced, a kind of common less than normal range (NR) lower limit
Clinical symptoms.Because red blood cell volume measure is more complicated, clinically often with hemoglobin(Hb)Concentration replaces.China's blood disease
Scholar is thought in China sea level area, adult male Hb < 120g/L, adult female(It is non-pregnant)Hb < 110g/L, pregnant woman Hb
< 100g/L just have anaemia.
The diagnostic criteria that WHO in 1972 is worked out thinks to be diagnosed as anaemia less than following levels in sea level area Hb:6 months
To the children of < 6 years old 110g/L, 6~14 years old children 120g/L, adult male 130g/L, adult female 120g/L, pregnant woman 110g/L.
It should be noted that it is height to occupy the hemoglobin normal value of highlands resident long compared with sea level resident;In gestation, Hypoproteinemia, fill
When courageous and upright heart failure, splenomegaly and macroglobulinemia, plasma volume increase, even if now red blood cell volume be it is normal,
But because blood is diluted, hemoglobin concentration reduces, and is easily misdiagnosed as anaemia;Circulating appearance is waited in dehydration or acute big lose blood
When amount is reduced, due to pachyemia, even if red blood cell volume is relatively low, but because hemoglobin concentration increases, anaemia is easily failed to pinpoint a disease in diagnosis.
Based on different clinical characters, anaemia has different classification.Such as:By the acute and chronic anaemia of anaemia tempo point;Press
Red cell morphology divides macrocytic anemia, normocytic anemia and microcytic hypochromic anemia;By hemoglobin concentration point
Slightly, moderate, severe and pole anemia;Divide hyperplastic anemia by the red system's proliferative conditions of marrow(Such as hemolytic anemia, iron-deficient
Anaemia, megaloblastic anemia etc.)With Hypolasia anaemia(Such as alpastic anemia).
Hemolytic anemia is due to the increase of hematoclasis speed(The lost of life), more than marrow hemopoiesis compensatory capacity and
The anaemia of generation.Marrow has 6~8 times of the compensatory potentiality of erythroid hematopoiesis.If hematoclasis speed is in the compensatory zone of marrow,
Though then there is haemolysis, anaemia, referred to as haemolytic disease are occurred without.About 120 days normocytic life-spans, only in red blood cell
Anaemia can just occur when the lost of life was to 15~20 days, the basic reason of hemolytic anemia is to shorten red blood cell life span.Cause red
The reason for cytoclasis accelerates, which can summarize, is divided into the latent defect and red blood cell external factor exception of red blood cell in itself.The former is mostly
Haemolytic diseases, the latter cause the cause of disease of acquired hemolytic anemia, the degree that blood oxygen carrying capacity declines, the journey that blood volume declines
Degree, the compensatory and tolerance of the systems such as speed and blood, circulation, the breathing of anaemia, which occurs, can influence the clinical table of anaemia
It is existing.The symptom occurred earliest has dizzy, weak, sleepy;And most common, most prominent sign is pale complexion.The weight of symptom
The degree of speed, anaemia and the compensatory capacity of body depending on anaemia.
Nineteen ninety, Frenchman Y. Lapierre, which publish an article, describes an immunology detection new technology:By antigen-antibody
Reaction is combined with gel molecular sieve technology, i.e. micro-column gel immunization experiment.The essence of the technology is hemagglutination test, i.e. red blood cell
Macroscopic immuno agglutination reaction occurs in the gel media of microtrabeculae intracavitary for antigen-antibody.
The essence of micro-column gel immune response is hemagglutination test (HA test), can also be referred to as micro-column gel hemagglutination test
(Microcolumn Gel Heamagglutination Assay, MGHA), based on biochemistry gel filtration technology and centrifugation
Technology and immunochemistry Ag-Ab idiosyncrasy.In micro-column gel pipe, after red blood cell and corresponding antibodies combination, formed red
Cell agglutination agglomerate.Under certain centrifugal force, the aggegation agglomerate is located in glue surface or glue;And if red blood cell is not and corresponding anti-
Body combines, and is still scattered red blood cell, under same centrifugal force, erythrocyte sedimentation to microtrabeculae tip bottom.Though its feature compared with
Conventional method cost is slightly higher;But its result is clear, be easy to interpretation, the degree of accuracy and susceptibility are high, standardization is loaded, standardization behaviour
Make, eliminate the interference of conventional method human factor, the advantages that for future reference can be preserved, be increasingly becoming the side that various big hospital generally uses
Method.
Kit, it is the box for holding the chemical reagent such as detection chemical composition, medicament residue, viral species.Typically
Hospital, pharmacy corporation use;The essence of micro-column gel immune response is hemagglutination test (HA test), can also be referred to as micro-column gel blood clotting
Experiment(Microcolumn Gel Heamagglutination Assay, MGHA), in micro-column gel pipe, red blood cell and phase
After answering antibody binding, erythrocyte agglutination agglomerate is formed.Under certain centrifugal force, the aggegation agglomerate is located in glue surface or glue;And
Red blood cell does not combine with corresponding antibodies, is still scattered red blood cell, under same centrifugal force, erythrocyte sedimentation to microtrabeculae tip
Bottom.In existing technology, preservation imperfection of the kit for reagent, such as some reagents need vacuum to preserve, in addition,
Reagent is after usage, it is necessary to carry out centrifugal treating, and in existing kit, only reagent preserves function, at centrifugation
Reason need it is extra find equipment, therefore, for it is above-mentioned the problem of, design a kind of new detection kit, just seem particularly heavy
Will.
The content of the invention
For problem above, the invention provides the detection of antibiotics induction type hemolytic anemia micro-column gel agglutination assay to try
Agent box, the environment that vacuum preserves can be provided detection reagent, and centrifugal treating service can be provided, can effectively solve to carry on the back
The problem of in scape technology.
To achieve the above object, the present invention provides following technical scheme:Antibiotics induction type hemolytic anemia is micro-
Post gel method detection kit, including box body and the Centrifugal Machine Control knob on the downside of box body, in addition to centrifugation cabin and micro-
Type vavuum pump, it is described centrifugation cabin inside motor and centrifugal shaft are installed, the centrifugal shaft lower end is connected with motor, it is described from
Mandrel upper end is provided with connecting rod, and the connecting rod is connected with rack;
The minipump is arranged on the inner right side bottom position of box body, and the right upper portion of the box body is also equipped with three
Reagent packaging container, the Reagent packaging container are respectively connected with airway tube, and magnetic valve is provided with the airway tube, and the magnetic valve is electrically connected with
Electromagnetic valve switch, the air guide pipe end are connected on minipump, and the minipump is electrically connected with vavuum pump and opened
Close, be provided with cooler equipped with contrast test paper, tray interior on the downside of the vacuum pump switch, ice bag is provided with cooler,
For adjusting temperature.
As a kind of preferable technical scheme of the present invention, the Reagent packaging container inside circumference posts cushion, the cushion
For foam.
By six groups it is support symmetrical two-by-two inside the centrifugation cabin as a kind of preferable technical scheme of the present invention
Disk.
As a kind of preferable technical scheme of the present invention, the magnetic valve and electromagnetic valve switch are each equipped with 3.
As a kind of preferable technical scheme of the present invention, the airway tube is connected to the bottom of Reagent packaging container.
As a kind of preferable technical scheme of the present invention, three electromagnetic valve switch and three Reagent packaging containers are one-to-one corresponding
Relation, the control system that three electromagnetic valve switch are accessed outside lid.
As a kind of preferable technical scheme of the present invention, centrifugation cabin hatchcover is provided with the top of the centrifugation cabin.
As a kind of preferable technical scheme of the present invention, the magnetic valve is Direct Action Type.
As a kind of preferable technical scheme of the present invention, the lid outer surface is made up of waterproof material, outside the lid
Portion is provided with control system and LED digital display screens.
Compared with prior art, the beneficial effects of the invention are as follows:
1. the present invention passes through rotary centrifuge control handle, control by the setting of motor, centrifugal shaft, connecting rod and rack
The speed of rotation of motor processed, change the size of centrifugal force, realize the centrifugal treating to detection reagent, offer centrifugation has been provided
The purpose of service is handled, reduces the trouble for finding centrifugation apparatus, saves the time, tray interior is provided with cooler, cooling
Ice bag is provided with device, for carrying out Cord blood to reagent.
2. being used cooperatively by airway tube, magnetic valve, electromagnetic valve switch and minipump of the invention, passes through vavuum pump
Switch control minipump opens or closes, and electromagnetic valve switch control magnetic valve opens or closes, so as to airway tube
Break-make be controlled, and then by Reagent packaging container air extract out, realize vacuum preservation purpose.
Brief description of the drawings
Fig. 1 is schematic structural view of the invention;
Fig. 2 is front section view of the present invention.
In figure:1- box bodys;2- Centrifugal Machine Control knobs;3- centrifuges cabin;4- centrifugal shafts;5- connecting rods;6- racks;7- electricity
Motivation;8- centrifuges cabin hatchcover;9- Reagent packaging containers;10- cushions;11- electromagnetic valve switch;12- vacuum pump switch;13- contrasts test paper;
14- minipumps;15- airway tubes;16- magnetic valves;17- hinges;18- lids;19- support frames.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art are obtained every other under the premise of creative work is not made
Embodiment, belong to the scope of protection of the invention.
Fig. 1 and Fig. 2 are referred to, the present invention provides a kind of technical scheme:Antibiotics induction type hemolytic anemia is micro-
Post gel method detection kit, including box body 1 and the Centrifugal Machine Control knob 2 installed in the downside of box body 1, in addition to centrifugation cabin 3
With minipump 14, centrifuging and motor 7 and centrifugal shaft 4 are installed inside cabin 3, the lower end of centrifugal shaft 4 is connected with motor 7, from
The upper end of mandrel 4 is provided with connecting rod 5, and connecting rod 5 is connected with rack 6, passes through motor 7, centrifugal shaft 4, connecting rod 5 and glove
The setting of frame 6, by rotary centrifuge control handle 2, the speed of rotation of motor 7 is controlled, changes the size of centrifugal force, realizes
To the centrifugal treating of detection reagent, reach the purpose that centrifugal treating service is provided, reduce the trouble for finding centrifugation apparatus,
Save the time.
Box body 1 is connected with lid 18 by hinge 17, and minipump 14 is arranged on the inner right side bottom position of box body 1
Put, the right upper portion of box body 1 is also equipped with three Reagent packaging containers 9, and Reagent packaging container 9 is respectively connected with airway tube 15, installed on airway tube 15
There is magnetic valve 16, magnetic valve 16 is electrically connected with electromagnetic valve switch 11, and the end of airway tube 15 is connected on minipump 14, micro-
Type vavuum pump 14 is electrically connected with vacuum pump switch 12, and the downside of vacuum pump switch 12 passes through airway tube equipped with contrast test paper 13
15th, magnetic valve 16, electromagnetic valve switch 11 and minipump 14 are used cooperatively, and are passed through vacuum pump switch 12 and are controlled micro vacuum
Pump 14 opens or closes, and the switch control magnetic valve 16 of magnetic valve 11 opens or closes, so as to enter to the break-make of airway tube 15
Row control, and then the air in Reagent packaging container 9 is extracted out, the purpose of vacuum preservation is realized, box body 1 is internally provided with cooler,
Ice bag is provided with cooler, for adjusting temperature.
The inside circumference of Reagent packaging container 9 posts cushion 10, and cushion 10 is foam, detection reagent can be played very well
Damping anticollision effect, magnetic valve 16 and electromagnetic valve switch 11 be each equipped with 3, can once carry 3 parts of detection examinations
Agent, airway tube 15 are connected to the bottom of Reagent packaging container 9, and the top of centrifugation cabin 3 is provided with centrifugation cabin hatchcover 8, can in electric motor operation
To play a protective role, magnetic valve 16 is Direct Action Type, easy to control, simple and fast.
The operation principle of the present invention:By rotary centrifuge control handle 2, control the speed of rotation of motor 7, change from
The size of mental and physical efforts, the centrifugal treating to detection reagent is realized, reached the purpose that centrifugal treating service is provided, reduced searching
The trouble of centrifugation apparatus, saves the time, passes through airway tube 15, magnetic valve 16, electromagnetic valve switch 11 and minipump 14
It is used cooperatively, opening or closing for minipump 14, the switch control magnetic valve of magnetic valve 11 is controlled by vacuum pump switch 12
16 open or close, so as to be controlled to the break-make of airway tube 15, and then the air in Reagent packaging container 9 is extracted out, realized
The purpose that vacuum preserves, the top of centrifugation cabin 3 are provided with centrifugation cabin hatchcover 8, can played a protective role in electric motor operation, from
Detection reagent after heart processing compares with contrast test paper 13, so as to obtain testing result.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement made within refreshing and principle etc., should be included in the scope of the protection.
Claims (9)
1. antibiotics induction type hemolytic anemia micro-column gel agglutination assay detection kit, including box body(1)With installed in box
Body(1)The Centrifugal Machine Control knob of downside(2), it is characterised in that:Also include centrifugation cabin(3)And minipump(14), it is described
Centrifuge cabin(3)Inside is provided with motor(7)And centrifugal shaft(4), the centrifugal shaft(4)Lower end and motor(7)It is connected, it is described
Centrifugal shaft(4)Upper end is provided with connecting rod(5), the connecting rod(5)It is connected with rack(6);
The box body(1)Pass through hinge(17)It is connected with lid(18), the minipump(14)It is arranged on box body(1)It is interior
Portion bottom right side position, the box body(1)Right upper portion be also equipped with three Reagent packaging containers(9), the Reagent packaging container(9)Pass through two
Layer support frame(19)It is fixed, the Reagent packaging container(9)It is respectively connected with airway tube(15), the airway tube(15)On magnetic valve is installed
(16), the magnetic valve(16)It is electrically connected with electromagnetic valve switch(11), the airway tube(15)End is connected to micro vacuum
Pump(14)On, the minipump(14)It is electrically connected with vacuum pump switch(12), the vacuum pump switch(12)Match somebody with somebody downside
Have contrast test paper(13), the box body(1)It is internally provided with cooler.
2. antibiotics induction type hemolytic anemia micro-column gel agglutination assay detection kit according to claim 1, its
It is characterised by:The Reagent packaging container(9)Inside circumference posts cushion(10), the cushion(10)For foam.
3. antibiotics induction type hemolytic anemia micro-column gel agglutination assay detection kit according to claim 1, its
It is characterised by:The centrifugation cabin(3)Inside is pallet symmetrical two-by-two by six groups.
4. antibiotics induction type hemolytic anemia micro-column gel agglutination assay detection kit according to claim 1, its
It is characterised by:The magnetic valve(16)And electromagnetic valve switch(11)It is each equipped with three.
5. antibiotics induction type hemolytic anemia micro-column gel agglutination assay detection kit according to claim 1, its
It is characterised by:The airway tube(15)It is connected to Reagent packaging container(9)Bottom.
6. antibiotics induction type hemolytic anemia micro-column gel agglutination assay detection kit according to claim 1, its
It is characterised by:Three electromagnetic valve switch(11)With three Reagent packaging containers(9)For one-to-one relationship, three magnetic valves are opened
Close(11)Access lid(18)Outside control system.
7. antibiotics induction type hemolytic anemia micro-column gel agglutination assay detection kit according to claim 1, its
It is characterised by:The centrifugation cabin(3)Top is provided with centrifugation cabin hatchcover(8).
8. antibiotics induction type hemolytic anemia micro-column gel agglutination assay detection kit according to claim 1, its
It is characterised by:The magnetic valve(16)For Direct Action Type.
9. antibiotics induction type hemolytic anemia micro-column gel agglutination assay detection kit according to claim 1, its
It is characterised by:The lid(18)Outer surface is made up of waterproof material, the lid(18)Outside is provided with control system and LED numbers
Aobvious screen.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
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CN201710769984.3A CN107478852B (en) | 2017-08-31 | 2017-08-31 | Antibiotics induction type hemolytic anemia micro-column gel agglutination assay detection kit |
CN201910052126.6A CN109633150B (en) | 2017-08-31 | 2017-08-31 | Micro-column gel method detection method for antibiotic drug-induced hemolytic anemia |
Applications Claiming Priority (1)
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CN201710769984.3A CN107478852B (en) | 2017-08-31 | 2017-08-31 | Antibiotics induction type hemolytic anemia micro-column gel agglutination assay detection kit |
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CN201910052126.6A Division CN109633150B (en) | 2017-08-31 | 2017-08-31 | Micro-column gel method detection method for antibiotic drug-induced hemolytic anemia |
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CN107478852B CN107478852B (en) | 2019-05-03 |
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CN201710769984.3A Active CN107478852B (en) | 2017-08-31 | 2017-08-31 | Antibiotics induction type hemolytic anemia micro-column gel agglutination assay detection kit |
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CN103406214B (en) * | 2013-08-30 | 2015-04-22 | 安徽信灵检验医学科技有限公司 | Biological sample safe centrifugal machine |
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CN203448193U (en) * | 2013-08-16 | 2014-02-26 | 合肥天一生物技术研究所 | Centrifugal machine for micro-column gel cards |
CN103675297A (en) * | 2013-12-13 | 2014-03-26 | 江苏中济万泰生物医药有限公司 | Medicament-induced hemolytic anemia detection kit and detection method thereof |
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CN106771284A (en) * | 2016-12-26 | 2017-05-31 | 张志飞 | A kind of semi-automatic micro-column gel card detecting system and detection method |
Also Published As
Publication number | Publication date |
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CN109633150B (en) | 2022-03-22 |
CN109633150A (en) | 2019-04-16 |
CN107478852B (en) | 2019-05-03 |
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