CN205898818U - Seven ally oneself with gold mark detects card - Google Patents
Seven ally oneself with gold mark detects card Download PDFInfo
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- CN205898818U CN205898818U CN201620726206.7U CN201620726206U CN205898818U CN 205898818 U CN205898818 U CN 205898818U CN 201620726206 U CN201620726206 U CN 201620726206U CN 205898818 U CN205898818 U CN 205898818U
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Abstract
The utility model provides a seven ally oneself with gold mark detects card, the utility model relates to a medical technology field, concretely relates to novel autoantibody seven ally oneself with gold mark detects card based on yeast reorganization expression autoantigen. For can be rapid provide comprehensive judgement the foundation for the doctor, the utility model provides a gold mark detects the box, the measuring box including last casing under with, the internal detecting element that is equipped with of inferior valve, last casing on be equipped with the observation window, detecting element including nitrocellulose membranes, the gold mark of locating the peridium of nitrocellulose membranes upper strata front end fills up, liquid absorption pads, sample pads, and the detection is taken. The beneficial effects of the utility model reside in that, jointly detection through seven kinds of autoantibodies to in serum or the plasma can improve systemic erythematosus, dry syndrome, mixed connective tissue disease, the diagnostic sensitiveness of myositis / dermatomyositis, in the stage that more is favorable to the multianalysis state of an illness to be located, judges there is important meaning to somatotype, pursuit and the prognosis of the state of an illness.
Description
Technical field
The present invention relates to medicine technology field and in particular to a kind of based on the recombinant expressed autoantigen of yeast new itself
Antibody seven gold medal mark detection card.
Background technology
Autoimmune disease is the most commonly seen disease of ranking the 3rd after cardiovascular disease and cancer, and it is totally sent out
Sick rate accounts for the 3%～5% of world population, and also in rising trend, and autoimmune disease scope is wide, including sle(system
System property lupus erythematosus), ss (dry syndrome), mixed connective tissue disease (mctd), polymyositiss/dermatomyositiss antibody (pm/
Dm) etc., various clinical symptoms have part crossover it is difficult to establish clear and definite criterion.
Anti-sm antibody has to the diagnosis of sle compared with high specific, is the serum mark antibody of the sle generally acknowledging at present,
Smd1, smb' polypeptide is modal anti-sm specific autoimmune response target, is conducive to the early diagnosiss of sle.Anti- ssa resists
Also known as anti-ro antibody (ant-roantibodies), resist drying syndrome antigen a antibody, anti-ssa antibody target antigen has 52kd to body
With two kinds of ribonucleoprotein (RNP) of 60kd., also known as anti-la antibody (anti-la antibodies), resist drying is comprehensive for anti-ssb antibody
Disease antigen b antibody, anti-ssa antibody and anti-ssb antibody usually occur simultaneously, the same with anti-ssa antibody, and anti-ssb antibody also can draw
Play neonatal lupus syndrome (nle), anti-ssb antibody such as occurs in other autoimmune diseasees, patient is often accompanied by secondary
Property dry syndrome.Anti- u1-snrnp antibody sees in mixed connective tissue disease (mctd) patient, anti-u1-snrnp antibody
Can occur in multiple rheumatisms.Anti- jo-1 antibody is that the serum mark of polymyositiss/dermatomyositiss antibody (pm/dm) resists
Body, and most of patients is with interstitial lung disease (ild) and polyarthritis or arthralgia etc., anti-jo-1 antibody is multiple flesh
The serum mark antibody of inflammation/dermatomyositiss antibody (pm/dm).
At present, the Method And Principle being used for autoantibody detection employing both at home and abroad mainly has indirect immunofluorescence, enzyme connection to exempt from
Epidemic disease method, chemoluminescence method and colloidal gold method.Indirect immunofluorescence needs skilled artisan to be operated, and takes not
Energy batch processing, chemiluminescence needs specialized analyzer, and expensive, and technology is still not mature enough, and euzymelinked immunosorbent assay (ELISA) is adopted mostly
Detected with 96 microwell plates, complex steps take, colloidal gold method is simple to operate, easy to use, detection speed fast, simultaneously because various
Clinical symptoms have part crossover, and each individually diagnosis is costly, take big it is difficult to doctor provide comprehensive descision according to
According to.
Content of the invention
Mentioned above for solving the problems, such as, therefore the present invention uses seven colloidal gold cards, and seven described colloidal gold cards can be simultaneously
Seven kinds of autoantibodys of detection, that is, anti-smd1 antibody, anti-smb' antibody, anti-ssa52kd antibody, anti-ssa60kd antibody, anti-ssb resist
Body, anti-u1-snrnp antibody, anti-jo-1 antibody, not only can individually detect systemic lupus erythematosus (sle), dry syndrome, mixing
Property connective tissue disease, the sensitivity of myositis/dermatomyositiss diagnosis, comprehensive reference is more beneficial for improving diagnosis rate.The present invention provides
Technical scheme is as follows:
A kind of seven gold medal mark detection boxes, described detection box includes upper shell and lower house, sets in described lower casing body
There is plural detector unit, row sets in housing described detector unit side by side.Described upper shell be provided with two with
The upper sample well for adding sample using cooperatively with the detector unit described in two or more respectively and for observation detection knot
The observation window of fruit；Described detector unit include on lower house isolation channel and in isolation channel bottom antigen coat
Nitrocellulose filter, located at the coated gold standard pad of nitrocellulose filter upper strata front end, behind nitrocellulose filter upper strata
The liquid absorption pad at end, the sample pad with sample well correspondence position in coated gold standard pad, and located at celluloid
The comparison band of film upper strata position corresponding with observation window and the detection band of one or more.
Further, described two or more detector unit includes to be examined for the dry syndrome detecting dry syndrome
Survey unit, the nitrocellulose filter of the antigen coat in described dry syndrome detector unit is ssa52kd antigen, ssb resists
The former nitrocellulose filter with ssa60kd antigen coat, corresponding detection band is the detection band of ssa52kd antigen coat, ssb resists
Primordial covering detection band and the detection band of ssa60kd antigen coat.
Further, described two or more detector unit includes the Combination for detecting mixed connective tissue disease
Connective tissue disease detector unit, the nitrocellulose filter of the antigen coat in described mixed connective tissue disease detector unit is
The nitrocellulose filter of u1-snrnp antigen coat, corresponding detection band is the detection band of u1-snrnp antigen coat.
Further, described two or more detector unit includes the systemic red for detecting system lupus erythematosus
Yabbi skin ulcer detector unit, the nitrocellulose filter of the antigen coat in described mixed connective tissue disease detector unit is smd1
Antigen and the nitrocellulose filter of smb' antigen coat, corresponding detection band is detection band and the smb' antigen of smd1 antigen coat
Coated detection band.
Further, described two or more detector unit includes for detecting the multiple of polymyositiss/dermatomyositiss
Property myositis/dermatomyositiss detector unit, the celluloid of the antigen coat in described polymyositiss/dermatomyositiss detector unit
Film is the nitrocellulose filter of jo-1 antigen coat, and corresponding detection band is the detection band of jo-1 antigen coat.
Further, described comparison carries as sheep anti mouse igg coated comparison band.
A kind of preparation method of seven gold medal mark detection boxes, described preparation method comprises the steps:
First, it is coated nitrocellulose filter
1.1) prepare and be coated liquid: by smd1, smb', ssa52, ssa60, ssb, u1-snrnp and jo-1 antigen, Yi Jiyang
What anti-Mus igg was configured to 0.5mg/ml 5mg/ml respectively is coated liquid；
1.2) be coated: nitrocellulose membrane is cut into piece, by 1.1) in the liquid that is coated that configured be coated on 4 nitrocellulose membranes
Upper: ssa52kd, ssa60kd, ssb to be comprised on a nitrocellulose membrane and sheep anti mouse igg is coated liquid, on a nitrocellulose membrane
Comprise u1-snrnp and sheep anti mouse igg and be coated liquid, nitrocellulose membrane comprises smd1, smb' and sheep anti mouse igg is coated liquid, one
Jo-1 and sheep anti mouse igg is comprised on bar nitrocellulose membrane and is coated liquid；
1.3) it is dried: the nitrocellulose membrane being coated bar is placed in 2 hours of 37 DEG C of freeze-day with constant temperature；
2nd, it is coated gold standard pad
2.1) the anti-human igg of labelling Mus: prepare the anti-human igg of 0.5mg/ml 5mg/ml Mus of colloid gold label；
2.2) be coated: coated gold standard pad is cut into diaphragm, by 2.1) in the solution for preparing be coated on coated gold mark
On pad；
2.3) it is dried: the gold standard pad bar that is coated being coated is placed in 2 hours of 37 DEG C of freeze-day with constant temperature；
3rd, fit
3.1st, by dried sample pad, coated gold standard pad, nitrocellulose filter and liquid absorption pad in order successively
Paste after combination on pvc plate, make detector bar；
4th, cut
4.1) band that the detector bar posting in (three) and isolation channel are used cooperatively；
5th, assemble
5.1) band sequence is sequentially loaded in isolation channel, closes tight upper casing and lower casing.
A kind of using method of seven gold medal mark detection boxes, comprises the steps: to be separately added into 100 μ l toward in four wells
Sample to be tested, after 15-20 minute from observation window judged result, if detection band and comparison band all develop the color, show to contain in sample
There is the specific antibody of detection band institute envelope antigen；If detection band does not develop the color, only comparison band colour developing, then show not comprise in sample
The specific antibody of detection band institute envelope antigen or concentration are not up to detectable level；If detection band and comparison band all do not develop the color,
It is invalid then to show to detect.
The beneficial effects of the present invention is, by the anti-smd1 antibody in serum or blood plasma, anti-smb' antibody, anti-
The joint of seven kinds of autoantibodys such as ssa52 antibody, anti-ssa60 antibody, anti-ssb antibody, anti-u1-snrnp antibody, anti-jo-1 antibody
Detection can improve systemic lupus erythematosus (sle), dry syndrome, mixed connective tissue disease, the sensitivity of myositis/dermatomyositiss diagnosis,
It is more beneficial for the stage residing for comprehensive analysing patient's condition, important meaning is determined with to the typing of the state of an illness, tracking and prognosis.
Brief description
Accompanying drawing 1 is the rough schematic of present invention detection card；
Fig. 2 is the a-a of the present invention to sectional view.
Specific embodiment
The present invention is described further below in conjunction with the accompanying drawings.
Embodiment 1
Refer to Fig. 1 and Fig. 2, a kind of seven gold medal mark detection boxes, described detection box includes upper shell and lower house, institute
The detection box stated includes in described lower casing body and is provided with 4 detector units (1,2,3,4), and described upper shell is provided with 4
The sample well 7 for adding sample that uses cooperatively with described detector unit (1,2,3,4) and for observing testing result
Observation window 11；Described detector unit (1,2,3,4) include on lower house isolation channel 5 and in isolation channel 5 bottom
Antigen coat nitrocellulose filter 10, located at the coated gold standard pad 8 of nitrocellulose filter 10 upper strata front end, located at nitric acid
The liquid absorption pad 9 of cellulose membrane 10 upper strata rear end, the sample pad 7 with sample well correspondence position in coated gold standard pad 8,
And the comparison band c located at nitrocellulose filter 10 upper strata position corresponding with observation window 11 and detection band t of one or more.Institute
The 4g detector unit stated includes the dry syndrome detector unit 1 for detecting dry syndrome, and described being dried is comprehensive
The nitrocellulose filter of the antigen coat in disease detector unit 1 is ssa52kd antigen, ssb antigen and ssa60kd antigen coat
Nitrocellulose filter, corresponding detection band be ssa52kd antigen coat detection band t1, ssb antigen coat detection band t2 and
Detection band t3 of ssa60kd antigen coat;For detecting the mixed connective tissue disease detector unit of mixed connective tissue disease
2, the nitrocellulose filter of the antigen coat in described mixed connective tissue disease detector unit 2 is u1-snrnp antigen coat
Nitrocellulose filter, corresponding detection band be u1-snrnp antigen coat detection band t4;For detecting system erythema wolf
The systemic lupus erythematosus (sle) detector unit 3 of skin ulcer, the nitric acid of the antigen coat in described mixed connective tissue disease detector unit 3
Cellulose membrane is the nitrocellulose filter of smd1 antigen and smb' antigen coat, and corresponding detection band is the inspection of smd1 antigen coat
Measuring tape t5 and detection band t6 of smb' antigen coat;For detecting the polymyositiss/dermatomyositiss inspection of polymyositiss/dermatomyositiss
Survey unit 4, the nitrocellulose filter of the antigen coat in described polymyositiss/dermatomyositiss detector unit is jo-1 antigen bag
The nitrocellulose filter of quilt, corresponding detection band is detection band t7 of jo-1 antigen coat;Described comparison carries as sheep anti mouse igg
Coated comparison band c.
Embodiment 2
Described seven gold medal mark detection blocking Preparation Methods are as follows:
First, it is coated nitrocellulose filter
1) prepare and be coated liquid: by smd1, smb', ssa52, ssa60, ssb, u1rnp and jo-1 antigen, and sheep anti mouse
Igg Quality Control is configured to be coated accordingly concentration, respectively 0.5mg/ml, 1 mg/ml, 1.5 mg/ml, 2 mg/ml, 2.5 mg/
ml、3 mg/ml、3.5 mg/ml、4 mg/ml、4.5 mg/ml、5mg/ml；
2) envelope antigen: according to the size of immunoblotting sample applicator, nitrocellulose membrane is cut into diaphragm, by physical absorption
With covalently bound mode, by 1) in the spotting solution that configured be coated on diaphragm with certain ordering, respectively wrap
Had a sample band of smd1, smb' antigen recombinant expressed using yeast, be coated with recombinant expressed using yeast
One sample band of ssa52kd, ssa60kd, ssb antigen, it is coated the galley proof that there be u1-snrnp recombinant expressed using yeast
Product band, it is coated the sample band of the jo-1 recombinant expressed using yeast, four nitrocellulose filters altogether, formed independent
Detection line；
3) it is dried: the film strip being coated is put into 2 hours of 37 DEG C of freeze-day with constant temperature；
2nd, it is coated gold standard pad
1) the anti-human igg of labelling Mus: prepare the anti-human igg of Mus of colloid gold label；
2) it is coated: according to the size of immunoblotting sample applicator, gold standard pad is cut into diaphragm, by way of physical absorption,
By 1) in the solution for preparing be coated on pad；
3) it is dried: the gold standard pad being coated bar is put into 2 hours of 37 DEG C of freeze-day with constant temperature；
3rd, pad pasting
1st, by dried nc film (nitrocellulose filter), gold standard pad and sample pad, liquid absorption pad according to certain order
The front of the pvc plate in biadhesive is pasted in combination, as shown in Figure 2；
Cutting
The diaphragm posting in (three) is cut into the band of one fixed width according to independent detection line；
5th, assemble
1) the little bar cutting into single part is loaded on gold mark shell according to order, closes tightly gold and put on lid.
Embodiment 3
During detection, detection card is lain against in laboratory table, is separately added into 100 with dropper or sample loading gun in four wells
μ l sample to be tested, after 15-20 minute from observation window judged result, if detection band and comparison band all develop the color, show in sample
Specific antibody containing detection band institute envelope antigen, if detection band does not develop the color, only comparison band colour developing, then show not wrap in sample
The specific antibody of the envelope antigen of institute containing detection band or concentration are not up to detectable level, if detection band and comparison band all do not show
Color, then it is invalid to show to detect.In actual operation, the effect of this detection card is very good, compares with independent detection, saves big
The time of amount and expense, and the evaluation reference of one synthesis of doctor can be given.
Claims (9)
1. a kind of seven gold medals mark detection boxes, described detection box includes upper shell and lower house it is characterised in that under described
Be provided with plural detector unit in housing, described upper shell be provided with two or more respectively with the inspection described in two or more
Survey the sample well for adding sample and the observation window for observing testing result that unit matching uses；Described detector unit
Include on lower house isolation channel and in isolation channel the antigen coat of bottom nitrocellulose filter, fine located at nitric acid
The coated gold standard pad of the plain film upper strata front end of dimension, located at the liquid absorption pad of nitrocellulose filter upper strata rear end, located at coated
Sample pad with sample well correspondence position in gold standard pad, and located at nitrocellulose filter upper strata position corresponding with observation window
Comparison band and the detection band of one or more.
2. detection box as claimed in claim 1 is it is characterised in that described two or more detector unit includes for detecting
The dry syndrome detector unit of dry syndrome, the cellulose nitrate of the antigen coat in described dry syndrome detector unit
Plain film is the nitrocellulose filter of ssa52kd antigen, ssb antigen and ssa60kd antigen coat, and corresponding detection band is
The detection band of the detection band of ssa52kd antigen coat, ssb antigen coat detection band and ssa60kd antigen coat.
3. detection box as claimed in claim 1 is it is characterised in that described two or more detector unit includes for detecting
The mixed connective tissue disease detector unit of mixed connective tissue disease, in described mixed connective tissue disease detector unit
The nitrocellulose filter of antigen coat is the nitrocellulose filter of u1-snrnp antigen coat, and corresponding detection band is u1-snrnp
The detection band of antigen coat.
4. detection box as claimed in claim 1 is it is characterised in that described two or more detector unit includes for detecting
The systemic lupus erythematosus (sle) detector unit of systemic lupus erythematosus (sle), the antigen in described mixed connective tissue disease detector unit
Coated nitrocellulose filter is the nitrocellulose filter of smd1 antigen and smb' antigen coat, and corresponding detection band resists for smd1
The detection band of primordial covering and the detection band of smb' antigen coat.
5. detection box as claimed in claim 1 is it is characterised in that described two or more detector unit includes for detecting
The polymyositiss of polymyositiss/dermatomyositiss/dermatomyositiss detector unit, described polymyositiss/dermatomyositiss detector unit
The nitrocellulose filter of interior antigen coat is the nitrocellulose filter of jo-1 antigen coat, and corresponding detection band is jo-1 antigen
Coated detection band.
6. detection box as claimed in claim 1 is it is characterised in that described comparison carries as sheep anti mouse igg coated comparison band.
7. detection box as claimed in claim 1 is it is characterised in that described coated gold standard pad is that the anti-human igg of Mus is coated
Gold standard pad.
8. detection box as claimed in claim 1 is it is characterised in that be provided with 4 detector units in described detection box.
9. detection box as claimed in claim 1 is it is characterised in that described detector unit is arranged side by side and set in housing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620726206.7U CN205898818U (en) | 2016-07-12 | 2016-07-12 | Seven ally oneself with gold mark detects card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620726206.7U CN205898818U (en) | 2016-07-12 | 2016-07-12 | Seven ally oneself with gold mark detects card |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205898818U true CN205898818U (en) | 2017-01-18 |
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ID=57772104
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620726206.7U Expired - Fee Related CN205898818U (en) | 2016-07-12 | 2016-07-12 | Seven ally oneself with gold mark detects card |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205898818U (en) |
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2016
- 2016-07-12 CN CN201620726206.7U patent/CN205898818U/en not_active Expired - Fee Related
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170118 Termination date: 20190712 |
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| CF01 | Termination of patent right due to non-payment of annual fee |