CN107474152A - The preparation method and Inonotus obliquus extract solution of a kind of Inonotus obliquus crude extract - Google Patents
The preparation method and Inonotus obliquus extract solution of a kind of Inonotus obliquus crude extract Download PDFInfo
- Publication number
- CN107474152A CN107474152A CN201710757866.0A CN201710757866A CN107474152A CN 107474152 A CN107474152 A CN 107474152A CN 201710757866 A CN201710757866 A CN 201710757866A CN 107474152 A CN107474152 A CN 107474152A
- Authority
- CN
- China
- Prior art keywords
- inonotus obliquus
- preparation
- crude extract
- container
- raw material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to the preparation field of extract solution, the preparation method and Inonotus obliquus extract solution of more particularly to a kind of Inonotus obliquus crude extract, wherein preparation method use UV treatment, improve the recovery rate of polysaccharide in Inonotus obliquus, and play a part of killing mould；The polysaccharide of Inonotus obliquus is protected by graphene, prevents the degraded of polysaccharide；By being concentrated after extracted many times, obtain a kind of polyoses content height and do not allow perishable Inonotus obliquus extract solution.The preparation method and Inonotus obliquus extract solution of a kind of Inonotus obliquus crude extract provided by the invention, the advantages of being preserved for a long time with extract solution polyoses content height, and extract out the Inonotus obliquus crude extract of genuineness, it is economical to have retained the due Synergy of the numerous active ingredients of Inonotus obliquus to greatest extent again, it is suitable for industrialized production.
Description
Technical field
The present invention relates to the preparation field of extract solution, the preparation method of more particularly to a kind of Inonotus obliquus crude extract and birch are brown
Pore fungi extract solution.
Background technology
Inonotus obliquus, also known as birch are fine and soft, CHAGA, category load daughter bacteria subphylum, Hymenomycetes, non-brown Zoopagales, Hymenochaetaceae,
Brown sleeping Pseudomonas.Its sclerotium is a kind of Medicinal fungi of Russia.16th century, the among the people of some countries of Eastern Europe just used this so far
The sclerotium of our bacterium carries out anti-curing cancers.Siberian Tatars prevents and treats heart disease, hepatopathy, stomach trouble and food with this bacterium
Road disease etc..They adhere to taking and have just reached more significant dietary function generally with making tea, decocting water, the homely gimmick such as steep in wine.
Inonotus obliquus has huge economic value and Research Prospects, has obvious work in terms of antitumor, anti-oxidant, raising immunity
With, while have significant curative effect in terms of the treatment to hypoglycemic, reducing blood lipid, diabetes and angiocardiopathy.Modern means of science and technology pair
Research is individually extracted in the effect of various composition, demonstrates the scientific principle of this medical value.Therefore, the pharmacology of Inonotus obliquus is ground
Study carefully and be significant.
Main active substances polysaccharide in the tunning of Inonotus obliquus at present, its polysaccharide extraction liquid have obvious hypoglycemic
Effect, reducing blood lipid, suppress tumour growth, also have in terms of the resistivity to promoting function of immune system, enhancing human body significant
Effect.
Application No. 02109803.4, publication date are the patent of invention of on 2 26th, 2003, disclose a kind of Inonotus obliquus
Extraction method of polysaccharides, but the polysaccharide that this method finally gives is only 1.99%.And point out to need to select in the extractive technique scheme
With without the Inonotus obliquus to go mouldy, this is due to that part mould is often attached with Inonotus obliquus, and mould is brown in the birch of prior art
It is difficult to be entirely killed in pore fungi extraction process, and mould will cause final product easily to occur rotten once existing, and shadow
Ring the preservation of product.
Agricultural University Of Anhui, one is provided in June, 2012《Inonotus obliquus liquid fermentation and polysaccharide extracting process are ground
Study carefully》Academic dissertation, the paper studied Fuscoporia obliqua polysaccharide extraction process, and the dry mycelia of Inonotus obliquus is used in paper
Body → be dried to carrying for constant weight → crushing → precise → rehydration hot water extraction → centrifugation → drying → Inonotus obliquus Thick many candies
Taking technique, and draw in 80 DEG C of Extracting temperature, extraction time 3.5h, liquid ratio 1:It is optimum extraction process under the conditions of 15, gained
Inonotus obliquus Thick many candies average recoveries are 35.89%, and polysaccharide relative amount is 2.33%；
Wherein, coarse polysaccharide extractive rate (%)=[Thick many candies dry weight (g)/Inonotus obliquus mycelium dry weight (g)] × 100%；
The relative amount (%) of the Fuscoporia obliqua polysaccharide=[amount of the amount (g) of Fuscoporia obliqua polysaccharide/Inonotus obliquus Thick many candies
(g)] × 100%.
As can be seen that in prior art in the extraction of the effective composition of Inonotus obliquus, it is low to there is polysaccharide extract rate
Problem, this be easily degraded with the polysaccharide that the polysaccharide inside Inonotus obliquus is difficult to effectively be extracted and extract it is relevant, although
In paper《The research of Inonotus obliquus liquid fermentation and polysaccharide extracting process》In it has been mentioned that some prevent what polysaccharide from degrading to arrange
Apply, but these measures are still difficult to effectively prevent polysaccharide from degrading.
The content of the invention
For the low and final product of the polysaccharide extract rate in the effective composition of Inonotus obliquus in solution background above technology
The problem of rotten easily occurs, the present invention provides a kind of preparation method of Inonotus obliquus crude extract, including following preparation process：
S100, Inonotus obliquus raw material and graphene be put into container；
S200, break process formation mixture M 1 is carried out to the Inonotus obliquus raw material in container and graphene；
S300, processing is irradiated to the M1 in container using ultraviolet；
S400, after stopping UV treatment, extraction processing is carried out to mixture M 1, obtains leaching liquor L；
S500, by obtained leaching liquor L carry out concentration, that is, Inonotus obliquus crude extract is prepared.
Further, in step S400, extraction processing comprises the following steps：
S410,20 DEG C~30 DEG C of normal-temperature water is added into container, carries out extraction processing, leaching liquor L1 is obtained after filtering,
Surplus solid mixture M2；
S420, into container in remaining solid mixing M2,70 DEG C~95 DEG C of hot water is added, carries out extraction processing, mistake
Leaching liquor L2, surplus solid mixture M3 are obtained after filter；
S430, into container in remaining solid mixing M3, inorganic solution is added, extraction processing is carried out, is obtained after filtering
Leaching liquor L3, surplus solid mixture M4；
S440, into container in remaining solid mixing M4, organic solution is added, extraction processing is carried out, is obtained after filtering
Leaching liquor L4, surplus solid mixture M5；
In S500 concentrations, leaching liquor L1, L2, L3, L4 are mixed, concentration is carried out, that is, the brown hole of birch is prepared
Bacterium crude extract.
Further, in step S200, Mechanical Crushing processing is carried out in 0~4 DEG C of environment, is crushed to 20~60 mesh.
Further, in step S410, during using normal temperature flooding, Inonotus obliquus raw material dry material quality and normal-temperature water
Feed liquid mass ratio is 1:3~1:8, extraction time is 2~5 hours.
Further, in step S420, during using hot water extraction, the feed liquid of Inonotus obliquus raw material dry material quality and hot water
Mass ratio is 1:5~1:10, extraction time is 1~5 hour.
Further, in step S430, when being extracted using inorganic solution, inorganic solution is salt solution；Wherein salt matter
Measure as 1 ‰~the 5 ‰ of Inonotus obliquus raw material dry material quality, the feed liquid matter of Inonotus obliquus raw material dry material quality and salt solution
Amount is than being 1:4~1:7, extraction time is 2~4 hours.
Further, in step S440, when being extracted using organic solution, organic solution is Inonotus obliquus raw material dry material matter
The ethanol of colourless acetic acid and Inonotus obliquus raw material the dry material quality 3%~11% of amount 1%~3%, extraction time are 1~4 small
When；
Wherein, the solubility of the colourless acetic acid is 5%~10%；The concentration of ethanol is 50%~75%.
Further, the inwall of the container is made of nonmetallic materials.
Further, the concentration carries out vacuum concentration processing, concentration using economic benefits and social benefits falling film type concentrator
To 38~45 Baume degrees.
A kind of Inonotus obliquus extract solution prepared by using the as above preparation method of any described Inonotus obliquus crude extract.
A kind of preparation method of Inonotus obliquus crude extract provided by the invention, using UV treatment, improve Inonotus obliquus
The recovery rate of middle polysaccharide, and play a part of killing mould；Protective effect is carried out to the polysaccharide in Inonotus obliquus by graphene,
Prevent the degraded of polysaccharide；By being concentrated after extracted many times, obtain a kind of polyoses content height and do not allow perishable Inonotus obliquus to carry
Take liquid.Inonotus obliquus crude extract liquid provided by the invention, there is the advantages of extract solution polyoses content height can preserve for a long time.
Embodiment
, below will be in the embodiment of the present invention to make the purpose, technical scheme and advantage of the embodiment of the present invention clearer
Technical scheme be clearly and completely described, it is clear that described embodiment is part of the embodiment of the present invention, rather than
Whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art are not making creative work premise
Lower obtained every other embodiment, belongs to the scope of protection of the invention.
The present invention provides a kind of preparation method of Inonotus obliquus crude extract, including following preparation process：
S100, Inonotus obliquus raw material and graphene be put into container；
S200, break process formation mixture M 1 is carried out to the Inonotus obliquus raw material in container and graphene；
S300, processing is irradiated to the M1 in container using ultraviolet；
S400, after stopping UV treatment, extraction processing is carried out to mixture M 1, obtains leaching liquor L；
S500, by obtained leaching liquor L carry out concentration, that is, Inonotus obliquus crude extract is prepared.
The broken dissolution with intracellular polyse of Inonotus obliquus can be promoted using ultraviolet, but ultraviolet can also be accelerated simultaneously
The degraded of polysaccharide, causes the recovery rate of polysaccharide to reduce.And graphene is added in the present invention, because the aperture of graphene is in 0.5nm
Between~200nm, the present invention uses graphene of the aperture between 100nm~200nm, and the diameter of polysaccharide exists in Inonotus obliquus
Between 10nm~150nm, and Inonotus obliquus and mould diameter are typically more than 2 μm.Therefore, the technical side used in the present invention
Case, after ultraviolet irradiation and break process Inonotus obliquus, the polysaccharide that is separated can inside " hide into " graphene, without
Influenceed by ultraviolet.Meanwhile the pore size of graphene does not allow Inonotus obliquus and mould to enter in graphene, thus it is purple
Outside line can be fully handled being permitted Inonotus obliquus and mould；Because the anti-uv-ray of mould is poor, at the place of ultraviolet
Under reason, mould can be killed.In addition, it can be handled carrying out extraction processing to mixture M 1 using ultrasound, due to birch
The polysaccharide of brown pore fungi can be such that polysaccharide is more fully extracted inside graphene inside graphene, using being ultrasonically treated,
Be advantageous to improve the recovery rate of Fuscoporia obliqua polysaccharide.
A kind of preparation method of Inonotus obliquus crude extract provided by the invention, using UV treatment, improve Inonotus obliquus
The recovery rate of middle polysaccharide, and play a part of killing mould；Protective effect is carried out to the polysaccharide in Inonotus obliquus by graphene,
Prevent the degraded of polysaccharide；By being concentrated after extracted many times, obtain a kind of polyoses content height and do not allow perishable Inonotus obliquus to carry
Take liquid.
Specifically, in step S400, extraction processing comprises the following steps：
S410,20 DEG C~30 DEG C of normal-temperature water is added into container, carries out extraction processing, leaching liquor L1 is obtained after filtering,
Surplus solid mixture M2；
S420, into container in remaining solid mixing M2,70 DEG C~95 DEG C of hot water is added, carries out extraction processing, mistake
Leaching liquor L2, surplus solid mixture M3 are obtained after filter；
S430, into container in remaining solid mixing M3, inorganic solution is added, extraction processing is carried out, is obtained after filtering
Leaching liquor L3, surplus solid mixture M4；
S440, into container in remaining solid mixing M4, organic solution is added, extraction processing is carried out, is obtained after filtering
Leaching liquor L4, surplus solid mixture M5；
In S500 concentrations, leaching liquor L1, L2, L3, L4 are mixed, concentration is carried out, that is, the brown hole of birch is prepared
Bacterium crude extract.
Specifically, in step S200, Mechanical Crushing processing is carried out in 0~4 DEG C of environment, is crushed to 20~60 mesh.
The industrialized broken plant equipment for being required for using metal material substantially, and the plant equipment of metal material is being transported
It is easily heating up during turning.At high operating temperatures, the physical chemistry of complexity easily occurs for the material contacted with metal material
React and occur rotten.So temperature is lower, this physical reactions more can be suppressed, but among actual production, if temperature mistake
Low, then the energy consumed is excessive, so 0-4 DEG C of temperature is set, it is the most economical and the most practical.In addition, the particle of material gets over size,
For the area that material contacts with metal with regard to smaller, rotten probability is also lower；Also, material particles are bigger, filter operation Vietnamese side
Just；But then it is that material particles are smaller among leaching step, easier leaching content, comprehensive consideration, 20-60 mesh passes through the most
Ji is practical.
Specifically, in step S410, during using normal temperature flooding, the material of Inonotus obliquus raw material dry material quality and normal-temperature water
Liquid mass ratio is 1:3~1:8, extraction time is 2~5 hours.
Specifically, in step S420, during using 70 DEG C~95 DEG C of hot water extractions, Inonotus obliquus raw material dry material quality and heat
The feed liquid mass ratio of water is 1:5~1:10, extraction time is 1~5 hour.Temperature is too high, and effective content is apt to deteriorate, and
Waste the energy；Temperature is too low, and many contents can not be extracted into solution, and leaching rate is excessively slow, is not suitable for industrial metaplasia
Production.
Specifically, in step S430, when being extracted using inorganic solution, inorganic solution is salt solution；Wherein salt quality
For 1 ‰~the 5 ‰ of Inonotus obliquus raw material dry material quality, the feed liquid quality of Inonotus obliquus raw material dry material quality and salt solution
Than for 1:4~1:7, extraction time is 2~4 hours.Wherein, the ionic effect of sodium chloride in saline solution, finite concentration model are utilized
The leaching rate of Inonotus obliquus content can be effectively improved by enclosing；The leaching of Inonotus obliquus content can not be improved by crossing the saline solution of low concentration
Extracting rate, and the saline solution of excessive concentrations then influences post-production.
Further, in step S440, when being extracted using organic solution, organic solution is Inonotus obliquus raw material dry material matter
The ethanol of colourless acetic acid and Inonotus obliquus raw material the dry material quality 3%~11% of amount 1%~3%, extraction time are 1~4 small
When；
Wherein, the solubility of the colourless acetic acid is 5%~10%；The concentration of ethanol is 50%~75%.
Preferably, the inwall of the container is made of nonmetallic materials.Metal material compares the chemistry of nonmetallic materials
Activity is higher, when logical acid solution liquid is placed in canister, it is possible to and metal ion is dissolved in acid solution, it is brown so as to influence birch
The quality of pore fungi extract solution.The container of the nonmetallic materials used for：Glass jar, ceramic cylinder, cement pit, wooden barrel, special plastic
Bucket etc..
Preferably, the concentration carries out vacuum concentration processing using economic benefits and social benefits falling film type concentrator, and concentration is extremely
38~45 Baume degrees.
A kind of Inonotus obliquus extract solution prepared by using the as above preparation method of any described Inonotus obliquus crude extract.
Inonotus obliquus crude extract prepared by preparation method provided by the invention has polyoses content high and can protected for a long time
The advantages of depositing.
The present invention also provides following examples：
Embodiment 1：
A kind of preparation method of Inonotus obliquus crude extract：
(1) 200kg Russia Inonotus obliquus and 10kg graphene are put into container under the conditions of 1 DEG C and are crushed to 50
Mesh, and using ultraviolet treatment with irradiation 1h；
(2) stop ultraviolet treatment with irradiation, above-mentioned Inonotus obliquus is placed among the container that inwall is nonmetallic materials, added
Enter normal-temperature water 1000kg, extract 2 hours, leaching liquor L1 is then filtered out into collection.
(3) 92 DEG C of hot water is added in upper step process container until the gross mass of leftover materials and water be 1000kg, guarantor
92 ± 2 DEG C of water temperature is held, extracts 3 hours, leaching liquor L2 is then filtered out into collection.
(4) 85 DEG C of hot water is added in upper step process container until the gross mass of leftover materials and water is 1100kg, is added
Enter salt 0.8kg, stir evenly, keep 85 ± 2 DEG C of water temperature, extract 3 hours, leaching liquor L3 is then filtered out into collection.
(5) 80 DEG C of hot water is added in upper step process container until the gross mass of leftover materials and water is 800kg, is added
Enter the acetic acid 4kg of 10% concentration, add the ethanol 10kg of 75% alcoholic strength, keep 80 ± 2 DEG C of water temperature, extract 3 hours, then
Leaching liquor L4 is filtered out into collection.
(6) all filtrate L1, L2, L3, L4 are collected and mixed, using the production of Wenzhou Chao Yu plant equipment Co., Ltd
Mixed liquor is concentrated into 42 baume by 2000L economic benefits and social benefits outer circulation concentrators, point 3 batches.
(7) sterile filling, Inonotus obliquus crude extract is made.
Embodiment 2：
A kind of preparation method of Inonotus obliquus crude extract：
(1) 500kg northeast Inonotus obliquus and 30kg graphene are put into container under the conditions of 4 DEG C and are crushed to 20 mesh,
And using ultraviolet treatment with irradiation 1.5h；
(2) stop ultraviolet treatment with irradiation, above-mentioned Inonotus obliquus is placed among the container that inwall is nonmetallic materials, added
Enter normal-temperature water 2500kg, extract 3 hours, leaching liquor L1 is then filtered out into collection.
(3) 85 DEG C of hot water is added in upper step process container until the gross mass of leftover materials and water be 2500kg, guarantor
85 ± 2 DEG C of water temperature is held, extracts 2 hours, leaching liquor L2 is then filtered out into collection.
(4) 75 DEG C of hot water is added in upper step process container until the gross mass of leftover materials and water is 2500kg, is added
Enter salt 0.5kg, stir evenly, keep 75 ± 2 DEG C of water temperature, extract 2 hours, leaching liquor L3 is then filtered out into collection.
(5) 70 DEG C of hot water is added in upper step process container until the gross mass of leftover materials and water is 2000kg, is added
Enter the acetic acid 5kg of 5% concentration, add the ethanol 20kg of 50% alcoholic strength, keep 70 ± 2 DEG C of water temperature, extract 2 hours, then
Leaching liquor L4 is filtered out into collection.
(6) all filtrate L1, L2, L3, L4 are collected and mixed, using the production of Wenzhou Chao Yu plant equipment Co., Ltd
Mixed liquor is concentrated into 39 baume by 2000L economic benefits and social benefits outer circulation concentrators, point 6 batches.
(7) sterile filling, Inonotus obliquus crude extract is made.
Embodiment 3：
A kind of preparation method of Inonotus obliquus crude extract：
(1) 200kg Russia Inonotus obliquus and 10kg graphene are put into container under the conditions of 1 DEG C and are crushed to 50
Mesh, and using ultraviolet treatment with irradiation 1h；
(2) stop ultraviolet treatment with irradiation, above-mentioned Inonotus obliquus is placed among the container that inwall is nonmetallic materials, added
Enter normal-temperature water 1000kg, extract 2 hours, using 0.5h is ultrasonically treated, leaching liquor L1 is then filtered out into collection.
(3) 92 DEG C of hot water is added in upper step process container until the gross mass of leftover materials and water be 1000kg, guarantor
92 ± 2 DEG C of water temperature is held, is extracted 3 hours, using 0.3h is ultrasonically treated, leaching liquor L2 is then filtered out into collection.
(4) 85 DEG C of hot water is added in upper step process container until the gross mass of leftover materials and water is 1100kg, is added
Enter salt 0.8kg, stir evenly, keep 85 ± 2 DEG C of water temperature, extract 3 hours, using 0.2h is ultrasonically treated, then by leaching liquor L3
Filter out collection.
(5) 80 DEG C of hot water is added in upper step process container until the gross mass of leftover materials and water is 800kg, is added
Enter the acetic acid 4kg of 10% concentration, add the ethanol 10kg of 75% alcoholic strength, keep 80 ± 2 DEG C of water temperature, extract 3 hours, use
0.2h is ultrasonically treated, leaching liquor L4 is then filtered out into collection.
(6) all filtrate L1, L2, L3, L4 are collected and mixed, using the production of Wenzhou Chao Yu plant equipment Co., Ltd
Mixed liquor is concentrated into 42 baume by 2000L economic benefits and social benefits outer circulation concentrators, point 3 batches.
(7) sterile filling, Inonotus obliquus crude extract is made.
Test one,
With reference to《The research of Inonotus obliquus liquid fermentation and polysaccharide extracting process》The method of offer, it is bent to carry out Glucose standards
The drafting of line.Using phend-sulphuric acid：Take 0.1g polysaccharide semifinished product deionized water dissolvings and be settled to mL.Take 0.1mL samples dilute
0.5mL is taken after 10 times in test tube, adds 6% phenol 1mL, the concentrated sulfuric acid is settled to 10mL, and 80 DEG C of thermostatted waters are put into after shaking up
Water-bath 20mi n in bath.OD values at 490nm are determined, repeats, averages three times.
Calculated using the 1/100 of Inonotus obliquus material quality as Inonotus obliquus dry weight, be prepared by various embodiments of the present invention
The 1/100 of Inonotus obliquus crude extract total amount, and with paper《The research of Inonotus obliquus liquid fermentation and polysaccharide extracting process》In
It is comparative example that prior art extraction process, which makees the Inonotus obliquus crude extract that extraction process is extracted, under identical temperature conditionss,
The absolute alcohol of 3 times of volumes of crude extract is added, stands 12h.Centrifugation, dried after collecting precipitation, produce Thick many candies dry weight.
Embodiment and comparative example are weighed into 0.05g Thick many candies dry weight sample constant volumes in 25mL volumetric flasks, draw 1.0mL
OD values at phend-sulphuric acid measure 490nm, bring glucose standard curve into and convert to obtain Fuscoporia obliqua polysaccharide content.
Wherein, coarse polysaccharide extractive rate (%)=[Thick many candies dry weight (g)/Inonotus obliquus dry weight (g)] × 100%；
The relative amount (%) of Fuscoporia obliqua polysaccharide=[amounts (g) of the amount (g) of Fuscoporia obliqua polysaccharide/Thick many candies] ×
100%, the wherein amount of Thick many candies is 0.05g.
The embodiment of the present invention 1~3 and paper《The research of Inonotus obliquus liquid fermentation and polysaccharide extracting process》In it is existing
Technology extraction process as a comparison case, to the Inonotus obliquus crude extract of extraction according to above-mentioned method of testing and calculation, obtains
Result it is as shown in table 1：
Table 1
It can be seen from Table 1 that the birch prepared by the preparation method of Inonotus obliquus crude extract provided in an embodiment of the present invention is brown
The extraction process institute that the coarse polysaccharide extractive rate of pore fungi crude extract and the relative amount of Fuscoporia obliqua polysaccharide provide compared with comparative example
The Inonotus obliquus crude extract of extraction is significantly increased.Further, embodiment 3 employs the processing mode of ultrasound, the brown hole of its birch
The relative amount of granulose has further raising again compared with embodiment 1 and embodiment 2.
Test two,
The Inonotus obliquus crude extract that the embodiment of the present invention of same volume and comparative example are extracted, in 28 DEG C of ± 1 DEG C of decentralizations
Enter in identical Potato-dextrose-agar medium, and culture medium be placed in gnotobasis and stood, maintain the circulation of air,
Fungi count (CFU/g) in 7 days, 14 days and 21 days observation culture mediums respectively；One of which culture medium is added without Inonotus obliquus
Crude extract, it is other consistent, enter line blank with reference to experiment.Test result is as shown in table 2：
Table 2
| Time | Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 | Blank control |
| 7 days | 7CFU/g | 6CFU/g | 8CFU/g | 19CFU/g | 3CFU/g |
| 14 days | 12CFU/g | 13CFU/g | 12CFU/g | 67CFU/g | 9CFU/g |
| 21 days | 23CFU/g | 22CFU/g | 25CFU/g | 98CFU/g | 13CFU/g |
It can be seen from Table 2 that the birch prepared by the preparation method of Inonotus obliquus crude extract provided in an embodiment of the present invention
The Inonotus obliquus crude extract prepared by preparation technology that brown pore fungi crude extract is provided comparative example is compared, and mould bacterial content is few, therefore
It can more for a long time preserve, there are significant progressive and huge market prospects compared with prior art.
Finally it should be noted that：Various embodiments above is merely illustrative of the technical solution of the present invention, rather than its limitations；To the greatest extent
The present invention is described in detail with reference to foregoing embodiments for pipe, it will be understood by those within the art that：Its according to
The technical scheme described in foregoing embodiments can so be modified, either which part or all technical characteristic are entered
Row equivalent substitution；And these modifications or replacement, the essence of appropriate technical solution is departed from various embodiments of the present invention technology
The scope of scheme.
Claims (10)
- A kind of 1. preparation method of Inonotus obliquus crude extract, it is characterised in that：Including following preparation process：S100, Inonotus obliquus raw material and graphene be put into container；S200, break process formation mixture M 1 is carried out to the Inonotus obliquus raw material in container and graphene；S300, processing is irradiated to the M1 in container using ultraviolet；S400, after stopping UV treatment, extraction processing is carried out to mixture M 1, obtains leaching liquor L；S500, by obtained leaching liquor L carry out concentration, that is, Inonotus obliquus crude extract is prepared.
- A kind of 2. preparation method of Inonotus obliquus crude extract according to claim 1, it is characterised in that：In step S400, Extraction processing comprises the following steps：S410,20 DEG C~30 DEG C of normal-temperature water is added into container, carry out extraction processing, leaching liquor L1 is obtained after filtering, it is remaining Solid mixture M2；S420, into container in remaining solid mixing M2,70 DEG C~95 DEG C of hot water is added, extraction processing is carried out, after filtering Obtain leaching liquor L2, surplus solid mixture M3；S430, into container in remaining solid mixing M3, inorganic solution is added, extraction processing is carried out, is extracted after filtering Liquid L3, surplus solid mixture M4；S440, into container in remaining solid mixing M4, organic solution is added, extraction processing is carried out, is extracted after filtering Liquid L4, surplus solid mixture M5；In S500 concentrations, leaching liquor L1, L2, L3, L4 are mixed, concentration is carried out, that is, it is thick that Inonotus obliquus is prepared Extract.
- A kind of 3. preparation method of Inonotus obliquus crude extract according to claim 1, it is characterised in that：In step S200, Mechanical Crushing processing is carried out in 0~4 DEG C of environment, is crushed to 20~60 mesh.
- A kind of 4. preparation method of Inonotus obliquus crude extract according to claim 2, it is characterised in that：In step S410, During using normal temperature flooding, the feed liquid mass ratio of Inonotus obliquus raw material dry material quality and normal-temperature water is 1:3~1:8, during extraction Between be 2~5 hours.
- A kind of 5. preparation method of Inonotus obliquus crude extract according to claim 2, it is characterised in that：In step S420, During using hot water extraction, the feed liquid mass ratio of Inonotus obliquus raw material dry material quality and hot water is 1:5~1:10, extraction time is 1~5 hour.
- A kind of 6. preparation method of Inonotus obliquus crude extract according to claim 2, it is characterised in that：In step S430, When being extracted using inorganic solution, inorganic solution is salt solution；Wherein salt quality is Inonotus obliquus raw material dry material quality 1 ‰~5 ‰, the feed liquid mass ratio of Inonotus obliquus raw material dry material quality and salt solution is 1:4~1:7, extraction time be 2~ 4 hours.
- A kind of 7. preparation method of Inonotus obliquus crude extract according to claim 2, it is characterised in that：In step S440, When being extracted using organic solution, colourless acetic acid and birch brown hole of the organic solution for Inonotus obliquus raw material dry material quality 1%~3% The ethanol of bacterium raw material dry material quality 3%~11%, extraction time are 1~4 hour；Wherein, the solubility of the colourless acetic acid is 5%~10%；The concentration of ethanol is 50%~75%.
- A kind of 8. preparation method of Inonotus obliquus crude extract according to any one of claim 1 or 2, it is characterised in that：Institute The inwall for stating container is made of nonmetallic materials.
- A kind of 9. preparation method of Inonotus obliquus crude extract according to any one of claim 1 or 2, it is characterised in that：Institute State concentration and vacuum concentration processing, concentration to 38~45 Baume degrees are carried out using economic benefits and social benefits falling film type concentrator.
- 10. a kind of a kind of Inonotus obliquus using as described in 1~7 any one prepared by preparation method of Inonotus obliquus crude extract carries Take liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710757866.0A CN107474152B (en) | 2017-08-29 | 2017-08-29 | A kind of preparation method and Inonotus obliquus extracting solution of Inonotus obliquus crude extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710757866.0A CN107474152B (en) | 2017-08-29 | 2017-08-29 | A kind of preparation method and Inonotus obliquus extracting solution of Inonotus obliquus crude extract |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107474152A true CN107474152A (en) | 2017-12-15 |
| CN107474152B CN107474152B (en) | 2019-05-17 |
Family
ID=60602921
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710757866.0A Active CN107474152B (en) | 2017-08-29 | 2017-08-29 | A kind of preparation method and Inonotus obliquus extracting solution of Inonotus obliquus crude extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107474152B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109431830A (en) * | 2018-12-18 | 2019-03-08 | 陈东升 | A kind of oxidation resistant natural composition, food, cosmetics, health care product and drug |
| CN111471120A (en) * | 2020-06-16 | 2020-07-31 | 北京赫尔默技术有限公司 | Preparation method and application of chondroitin sulfate |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103467613A (en) * | 2013-09-10 | 2013-12-25 | 上海瑞丰农业科技有限公司 | Preparation method and product of agaricus blazeimurill (ABM) compound polysaccharide |
| CN104004106A (en) * | 2014-04-28 | 2014-08-27 | 杨庆贺 | Method for extracting effective substances in inonotus obliquus and preparation method for teabag |
-
2017
- 2017-08-29 CN CN201710757866.0A patent/CN107474152B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103467613A (en) * | 2013-09-10 | 2013-12-25 | 上海瑞丰农业科技有限公司 | Preparation method and product of agaricus blazeimurill (ABM) compound polysaccharide |
| CN104004106A (en) * | 2014-04-28 | 2014-08-27 | 杨庆贺 | Method for extracting effective substances in inonotus obliquus and preparation method for teabag |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109431830A (en) * | 2018-12-18 | 2019-03-08 | 陈东升 | A kind of oxidation resistant natural composition, food, cosmetics, health care product and drug |
| CN109431830B (en) * | 2018-12-18 | 2021-07-27 | 陈东升 | Antioxidant natural composition, food, cosmetic, health product and medicine |
| CN111471120A (en) * | 2020-06-16 | 2020-07-31 | 北京赫尔默技术有限公司 | Preparation method and application of chondroitin sulfate |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107474152B (en) | 2019-05-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112007057B (en) | Preparation method of wall-broken ganoderma lucidum spore powder capsule and wall-broken ganoderma lucidum spore powder capsule | |
| CN107474152B (en) | A kind of preparation method and Inonotus obliquus extracting solution of Inonotus obliquus crude extract | |
| CN108250320B (en) | Low-ash ganoderma lucidum polysaccharide extract and preparation method thereof | |
| CN106318600A (en) | Method for preparing ganoderma spore oil | |
| CN106832045B (en) | A kind of method that gingko episperm extracts pectin and phenolic acid compound simultaneously | |
| CN105455129B (en) | Natural bionic process based on cold chain, new product produced by natural bionic process based on cold chain and application | |
| CN103772460A (en) | Extraction process of cordycepin (active substance) from silkworm pupa cordyceps militaris | |
| CN106318611A (en) | Method for extracting ganoderma spore oil | |
| CN104877039B (en) | A kind of method that suppression concentration process foam of solution decompression containing Saponin produces | |
| CN104151439A (en) | Preparation method of paecilomyces hepialid mycelium polysaccharide | |
| CN111471859B (en) | Method for leaching uranium from low-grade uranium ore by macro-particle reinforced aspergillus niger | |
| CN107047862A (en) | A kind of Uygur's health protection tea extracting technology with immunoloregulation function | |
| CN111096222B (en) | Method for improving content of ginsenoside in adventitious roots of ginseng by using immobilized chaetomium globosum spores | |
| CN104830535A (en) | Onion essential oil and its extraction method and application | |
| CN105902577A (en) | Low-sugar Cordyceps militaris oral tablet and preparation method thereof | |
| CN107486166B (en) | Composite adsorbent and preparation method thereof | |
| CN104873553A (en) | Preparation method for hippophae rhamnoides active extract and application of hippophae rhamnoides active extract to inhibition of helicobacter pylori | |
| CN101444557B (en) | Method for producing general flavone of medicinal mulberry by supercritical carbon dioxide extraction | |
| CN106890191A (en) | A kind of biological concocting method of nanometer-size realgar | |
| CN105079430A (en) | Oral liquid for enhancing immunity to treat chronic bronchitis and preparation process thereof | |
| CN110898083A (en) | Propolis extract and preparation method thereof | |
| CN105125646A (en) | Application of trichoderma viride in improving of extraction rate of quercetin in daphniphyllum calycinum and ciderage composition medicinal material | |
| CN108546590A (en) | Application process of Fructus Perillae quintessence oil during citrus storage at normal temperature | |
| CN110638033B (en) | Organic silicon/palmetto sansevieria polysaccharide nano-composite, preparation method and application | |
| CN114807242B (en) | Method for extracting iodine from seaweed |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |