CN107474152B - A kind of preparation method and Inonotus obliquus extracting solution of Inonotus obliquus crude extract - Google Patents
A kind of preparation method and Inonotus obliquus extracting solution of Inonotus obliquus crude extract Download PDFInfo
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- 241000414067 Inonotus obliquus Species 0.000 title claims abstract description 121
- 239000000287 crude extract Substances 0.000 title claims abstract description 47
- 238000002360 preparation method Methods 0.000 title claims abstract description 35
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 20
- 229910021389 graphene Inorganic materials 0.000 claims abstract description 20
- 239000000284 extract Substances 0.000 claims abstract description 16
- 238000000605 extraction Methods 0.000 claims description 46
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 44
- 239000000463 material Substances 0.000 claims description 42
- 238000002386 leaching Methods 0.000 claims description 36
- 239000000243 solution Substances 0.000 claims description 33
- 238000012545 processing Methods 0.000 claims description 29
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 27
- 239000002994 raw material Substances 0.000 claims description 24
- 238000000034 method Methods 0.000 claims description 23
- 239000007788 liquid Substances 0.000 claims description 22
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 20
- 239000008247 solid mixture Substances 0.000 claims description 12
- 235000018185 Betula X alpestris Nutrition 0.000 claims description 11
- 235000018212 Betula X uliginosa Nutrition 0.000 claims description 11
- 238000001914 filtration Methods 0.000 claims description 10
- 239000007787 solid Substances 0.000 claims description 10
- 235000019441 ethanol Nutrition 0.000 claims description 9
- 238000002156 mixing Methods 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 8
- 241000894006 Bacteria Species 0.000 claims description 6
- 239000012266 salt solution Substances 0.000 claims description 6
- 150000003839 salts Chemical class 0.000 claims description 6
- 238000003809 water extraction Methods 0.000 claims description 4
- 239000011552 falling film Substances 0.000 claims description 3
- 150000004676 glycans Chemical class 0.000 abstract description 43
- 229920001282 polysaccharide Polymers 0.000 abstract description 43
- 239000005017 polysaccharide Substances 0.000 abstract description 43
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 238000011084 recovery Methods 0.000 abstract description 6
- 230000015556 catabolic process Effects 0.000 abstract description 4
- 238000006731 degradation reaction Methods 0.000 abstract description 4
- 238000004321 preservation Methods 0.000 abstract description 3
- 230000007774 longterm Effects 0.000 abstract description 2
- 230000000717 retained effect Effects 0.000 abstract 1
- 235000009508 confectionery Nutrition 0.000 description 9
- 241000233866 Fungi Species 0.000 description 8
- 241000747105 Fuscoporia Species 0.000 description 8
- 239000011148 porous material Substances 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 6
- 238000011160 research Methods 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 238000000855 fermentation Methods 0.000 description 5
- 230000004151 fermentation Effects 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- 239000007769 metal material Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 238000009210 therapy by ultrasound Methods 0.000 description 4
- 230000001476 alcoholic effect Effects 0.000 description 3
- 239000000706 filtrate Substances 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 238000012859 sterile filling Methods 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 241001558929 Sclerotium <basidiomycota> Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 239000012467 final product Substances 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 230000002218 hypoglycaemic effect Effects 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 239000001117 sulphuric acid Substances 0.000 description 2
- 238000002604 ultrasonography Methods 0.000 description 2
- 241000222382 Agaricomycotina Species 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 241000123392 Hymenochaetaceae Species 0.000 description 1
- 206010054949 Metaplasia Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 241001122767 Theaceae Species 0.000 description 1
- 241001314279 Zoopagales Species 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 208000019622 heart disease Diseases 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 208000019423 liver disease Diseases 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 230000015689 metaplastic ossification Effects 0.000 description 1
- 239000001965 potato dextrose agar Substances 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000011265 semifinished product Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000004614 tumor growth Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to the preparation field of extracting solution, in particular to the preparation method and Inonotus obliquus extracting solution of a kind of Inonotus obliquus crude extract, wherein preparation method uses UV treatment, improves the recovery rate of polysaccharide in Inonotus obliquus, and plays the role of killing mould；It is protected by polysaccharide of the graphene to Inonotus obliquus, prevents the degradation of polysaccharide；By being concentrated after extracted many times, it is high and do not allow perishable Inonotus obliquus extracting solution to obtain a kind of polyoses content.The preparation method and Inonotus obliquus extracting solution of a kind of Inonotus obliquus crude extract provided by the invention, have the advantages that extracting solution polyoses content height being capable of long-term preservation, and extract out genuine Inonotus obliquus crude extract, it is economical to have retained the due Synergy of the numerous effective components of Inonotus obliquus to greatest extent again, it is suitable for industrialized production.
Description
Technical field
The present invention relates to the preparation field of extracting solution, in particular to the preparation method of a kind of Inonotus obliquus crude extract and birch are brown
Pore fungi extracting solution.
Background technique
Inonotus obliquus, also known as birch be fine and soft, inonotus obliquus, belong to load daughter bacteria subphylum, Hymenomycetes, non-brown Zoopagales, Hymenochaetaceae,
Brown sleeping Pseudomonas.Its sclerotium is a kind of Medicinal fungi of Russia.16th century, some the civil of country in Eastern Europe just used this so far
The sclerotium of our bacterium carries out anti-curing cancers.Siberian Tatars prevents and treats heart disease, hepatopathy, stomach trouble and food with this bacterium
Road disease etc..They adhere to taking and have just reached more significant dietary function usually with making tea, decocting water, the homely gimmick such as steep in wine.
Inonotus obliquus has huge economic value and Research Prospects, has obvious work in terms of antitumor, anti-oxidant, raising immunity
With, while having significant curative effect to the treatment of hypoglycemic, reducing blood lipid, diabetes and cardiovascular disease aspect.Modern means of science and technology pair
Research is individually extracted in the effect of various composition, demonstrates the scientific principle of this medical value.Therefore, the pharmacology of Inonotus obliquus is ground
Study carefully and is of great significance.
Main active substances polysaccharide in the tunning of Inonotus obliquus at present, polysaccharide extraction liquid have apparent hypoglycemic
Effect, reducing blood lipid inhibit tumour growth, significant to promoting the resistivity aspect of function of immune system, enhancing human body also to have
Effect.
Application No. is 02109803.4, the patent of invention that publication date is on 2 26th, 2003 discloses a kind of Inonotus obliquus
Extraction method of polysaccharides, however the finally obtained polysaccharide of this method is only 1.99%.And it points out to need to select in the extractive technique scheme
With the Inonotus obliquus of no mildew, this is because part mould is often attached in Inonotus obliquus, and mould is brown in the birch of the prior art
It is difficult to be entirely killed in pore fungi extraction process, and mould will lead to final product and be easy to happen rotten, and shadow once existing
Ring the preservation of product.
Agricultural University Of Anhui, providing one in June, 2012, " Inonotus obliquus liquid fermentation and polysaccharide extracting process are ground
Study carefully " academic dissertation, which studies Fuscoporia obliqua polysaccharide extraction process, and the dry mycelia of Inonotus obliquus is used in paper
Body → drying to constant weight → crushing → precise → rehydration hot water extraction → centrifugation → drying → Inonotus obliquus Thick many candies mention
Taking technique, and obtain under the conditions of 80 DEG C of Extracting temperature, extraction time 3.5h, liquid-to-solid ratio 1:15 as optimum extraction process, gained
Inonotus obliquus Thick many candies average recoveries are 35.89%, and polysaccharide relative amount is 2.33%；
Wherein, coarse polysaccharide extractive rate (%)=[Thick many candies dry weight (g)/Inonotus obliquus mycelium dry weight (g)] × 100%；
The relative amount (%) of the Fuscoporia obliqua polysaccharide=[amount (g) of Fuscoporia obliqua polysaccharide/Inonotus obliquus Thick many candies amount
(g)] × 100%.
As can be seen that it is low that there are polysaccharide extract rates in the extraction of prior art ingredient effective for Inonotus obliquus
Problem, this be difficult to effectively be extracted with the polysaccharide inside Inonotus obliquus and the polysaccharide that extracts be easy to be degraded it is related, although
It has been mentioned that some prevent what polysaccharide from degrading to arrange in paper " research of Inonotus obliquus liquid fermentation and polysaccharide extracting process "
It applies, but these measures are still difficult to that polysaccharide is effectively prevent to degrade.
Summary of the invention
For the low and final product of the polysaccharide extract rate in the effective ingredient of Inonotus obliquus in solution background above technology
It is easy to happen rotten problem, the present invention provides a kind of preparation method of Inonotus obliquus crude extract, including following preparation step:
S100, Inonotus obliquus raw material and graphene are put into container；
S200, in container Inonotus obliquus raw material and graphene carry out break process formed mixture M 1；
S300, processing is irradiated to the M1 in container using ultraviolet light；
S400, after stopping UV treatment, extraction processing is carried out to mixture M 1, obtains leaching liquor L；
S500, obtained leaching liquor L is subjected to concentration, that is, Inonotus obliquus crude extract is prepared.
Further, in step S400, extraction processing the following steps are included:
S410, the normal-temperature water that 20 DEG C~30 DEG C are added into container, carry out extraction processing, leaching liquor L1 are obtained after filtering,
Surplus solid mixture M2；
S420, into container in remaining solid mixing M2,70 DEG C~95 DEG C of hot water is added, carries out extraction processing, mistake
Leaching liquor L2, surplus solid mixture M3 are obtained after filter；
S430, into container in remaining solid mixing M3, inorganic solution is added, carries out extraction processing, is obtained after filtering
Leaching liquor L3, surplus solid mixture M4；
S440, into container in remaining solid mixing M4, organic solution is added, carries out extraction processing, is obtained after filtering
Leaching liquor L4, surplus solid mixture M5；
In S500 concentration, leaching liquor L1, L2, L3, L4 are mixed, concentration is carried out, that is, the brown hole of birch is prepared
Bacterium crude extract.
Further, in step S200, Mechanical Crushing processing is carried out in 0~4 DEG C of environment, is crushed to 20~60 mesh.
Further, in step S410, when using room temperature flooding, Inonotus obliquus raw material dry material quality and normal-temperature water
Feed liquid mass ratio is 1:3~1:8, and extraction time is 2~5 hours.
Further, in step S420, when using hot water extraction, the feed liquid of Inonotus obliquus raw material dry material quality and hot water
Mass ratio is 1:5~1:10, and extraction time is 1~5 hour.
Further, in step S430, when being extracted using inorganic solution, inorganic solution is salt solution；Wherein salt matter
Amount is 1 ‰~the 5 ‰ of Inonotus obliquus raw material dry material quality, the feed liquid matter of Inonotus obliquus raw material dry material quality and salt solution
For amount than being 1:4~1:7, extraction time is 2~4 hours.
Further, in step S440, when being extracted using organic solution, organic solution is Inonotus obliquus raw material dry material matter
The colourless acetic acid of amount 1%~3% and the ethyl alcohol of Inonotus obliquus raw material dry material quality 3%~11%, extraction time are 1~4 small
When；
Wherein, the solubility of the colourless acetic acid is 5%~10%；The concentration of ethyl alcohol is 50%~75%.
Further, the inner wall of the container is made of nonmetallic materials.
Further, the concentration carries out vacuum concentration processing, concentration using economic benefits and social benefits falling film type concentrator
To 38~45 Baume degrees.
It is a kind of to use Inonotus obliquus extracting solution prepared by the as above preparation method of any Inonotus obliquus crude extract.
A kind of preparation method of Inonotus obliquus crude extract provided by the invention improves Inonotus obliquus using UV treatment
The recovery rate of middle polysaccharide, and play the role of killing mould；Protective effect is carried out to the polysaccharide in Inonotus obliquus by graphene,
Prevent the degradation of polysaccharide；By being concentrated after extracted many times, it is high and perishable Inonotus obliquus is not allowed to mention to obtain a kind of polyoses content
Take liquid.Inonotus obliquus crude extract liquid provided by the invention has the advantages that extracting solution polyoses content height being capable of long-term preservation.
Specific embodiment
It in order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below will be in the embodiment of the present invention
Technical solution be clearly and completely described, it is clear that described embodiments are some of the embodiments of the present invention, rather than
Whole embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not making creative work premise
Under every other embodiment obtained, shall fall within the protection scope of the present invention.
The present invention provides a kind of preparation method of Inonotus obliquus crude extract, including following preparation step:
S100, Inonotus obliquus raw material and graphene are put into container；
S200, in container Inonotus obliquus raw material and graphene carry out break process formed mixture M 1；
S300, processing is irradiated to the M1 in container using ultraviolet light；
S400, after stopping UV treatment, extraction processing is carried out to mixture M 1, obtains leaching liquor L；
S500, obtained leaching liquor L is subjected to concentration, that is, Inonotus obliquus crude extract is prepared.
Inonotus obliquus can be promoted broken using ultraviolet light and the dissolution of intracellular polyse, however ultraviolet light can also be accelerated simultaneously
The degradation of polysaccharide causes the recovery rate of polysaccharide to reduce.And graphene is added in the present invention, since the aperture of graphene is in 0.5nm
Between~200nm, the present invention uses graphene of the aperture between 100nm~200nm, and the diameter of polysaccharide exists in Inonotus obliquus
Between 10nm~150nm, and Inonotus obliquus and mould diameter are generally at 2 μm or more.Therefore, the technical side used in the present invention
Case, after ultraviolet light irradiation and break process Inonotus obliquus, the polysaccharide that is separated can inside " hide into " graphene, without
It is influenced by ultraviolet light.Meanwhile the pore size of graphene does not allow Inonotus obliquus and mould to enter in graphene, therefore purple
Outside line can sufficiently be handled Inonotus obliquus perhaps and mould；Since the anti-uv-ray of mould is poor, at the place of ultraviolet light
Under reason, mould can be killed.In addition, can be handled using ultrasound carrying out extraction processing to mixture M 1, due to birch
The polysaccharide of brown pore fungi can be such that polysaccharide is more fully extracted inside graphene inside graphene, using ultrasonic treatment,
Be conducive to improve the recovery rate of Fuscoporia obliqua polysaccharide.
A kind of preparation method of Inonotus obliquus crude extract provided by the invention improves Inonotus obliquus using UV treatment
The recovery rate of middle polysaccharide, and play the role of killing mould；Protective effect is carried out to the polysaccharide in Inonotus obliquus by graphene,
Prevent the degradation of polysaccharide；By being concentrated after extracted many times, it is high and perishable Inonotus obliquus is not allowed to mention to obtain a kind of polyoses content
Take liquid.
Specifically, in step S400, extraction processing the following steps are included:
S410, the normal-temperature water that 20 DEG C~30 DEG C are added into container, carry out extraction processing, leaching liquor L1 are obtained after filtering,
Surplus solid mixture M2；
S420, into container in remaining solid mixing M2,70 DEG C~95 DEG C of hot water is added, carries out extraction processing, mistake
Leaching liquor L2, surplus solid mixture M3 are obtained after filter；
S430, into container in remaining solid mixing M3, inorganic solution is added, carries out extraction processing, is obtained after filtering
Leaching liquor L3, surplus solid mixture M4；
S440, into container in remaining solid mixing M4, organic solution is added, carries out extraction processing, is obtained after filtering
Leaching liquor L4, surplus solid mixture M5；
In S500 concentration, leaching liquor L1, L2, L3, L4 are mixed, concentration is carried out, that is, the brown hole of birch is prepared
Bacterium crude extract.
Specifically, in step S200, Mechanical Crushing processing is carried out in 0~4 DEG C of environment, is crushed to 20~60 mesh.
The industrialized broken mechanical equipment for requiring to use metal material substantially, and the mechanical equipment of metal material is being transported
It is easy during turning heating up.At high operating temperatures, the material contacted with metal material is easy to happen complicated physical chemistry
It reacts and occurs rotten.So temperature is lower, it can more inhibit this physical reactions, but in actual production, if temperature mistake
Low, then the energy consumed is excessive, so the temperature of 0-4 DEG C of setting, the most economical and the most practical.In addition, the particle of material gets over size,
For the area that material is contacted with metal with regard to smaller, rotten probability is also lower；Also, material particles are bigger, filter operation Vietnamese side
Just；It is then that material particles are smaller but in leaching step, easier leaching content, comprehensive consideration, 20-60 mesh passes through the most
It helps practical.
Specifically, in step S410, when using room temperature flooding, the material of Inonotus obliquus raw material dry material quality and normal-temperature water
Liquid mass ratio is 1:3~1:8, and extraction time is 2~5 hours.
Specifically, in step S420, when using 70 DEG C~95 DEG C of hot water extractions, Inonotus obliquus raw material dry material quality and heat
The feed liquid mass ratio of water is 1:5~1:10, and extraction time is 1~5 hour.Temperature is excessively high, and effective content is apt to deteriorate, and
Waste of energy；Temperature is too low, and many contents can not be extracted into solution, and leaching rate is excessively slow, is not suitable for industry metaplasia
It produces.
Specifically, in step S430, when being extracted using inorganic solution, inorganic solution is salt solution；Wherein salt quality
It is 1 ‰~the 5 ‰ of Inonotus obliquus raw material dry material quality, the feed liquid quality of Inonotus obliquus raw material dry material quality and salt solution
Than for 1:4~1:7, extraction time is 2~4 hours.Wherein, the ionic effect of sodium chloride in saline solution, a certain concentration model are utilized
Enclose the leaching rate that can effectively improve Inonotus obliquus content；The saline solution for crossing low concentration can not improve the leaching of Inonotus obliquus content
Extracting rate, and the saline solution of excessive concentrations then influences post-production.
Further, in step S440, when being extracted using organic solution, organic solution is Inonotus obliquus raw material dry material matter
The colourless acetic acid of amount 1%~3% and the ethyl alcohol of Inonotus obliquus raw material dry material quality 3%~11%, extraction time are 1~4 small
When；
Wherein, the solubility of the colourless acetic acid is 5%~10%；The concentration of ethyl alcohol is 50%~75%.
Preferably, the inner wall of the container is made of nonmetallic materials.Metal material compares the chemistry of nonmetallic materials
Activity is higher, when logical acid solution liquid is placed in canister, it is possible to be dissolved in metal ion in acid solution, so that it is brown to influence birch
The quality of pore fungi extracting solution.The container of the nonmetallic materials of use are as follows: glass jar, ceramic cylinder, cement pit, wooden barrel, special plastic
Bucket etc..
Preferably, the concentration carries out vacuum concentration processing using economic benefits and social benefits falling film type concentrator, and concentration is extremely
38~45 Baume degrees.
It is a kind of to use Inonotus obliquus extracting solution prepared by the as above preparation method of any Inonotus obliquus crude extract.
Inonotus obliquus crude extract prepared by preparation method provided by the invention has polyoses content high and can protect for a long time
The advantages of depositing.
The present invention also provides following embodiments:
Embodiment 1:
A kind of preparation method of Inonotus obliquus crude extract:
(1) graphene of 200kg Russia Inonotus obliquus and 10kg are put into container under the conditions of 1 DEG C and are crushed to 50
Mesh, and use ultraviolet treatment with irradiation 1h；
(2) stop ultraviolet treatment with irradiation, above-mentioned Inonotus obliquus is placed in the container that inner wall is nonmetallic materials, is added
Enter normal-temperature water 1000kg, extracts 2 hours, leaching liquor L1 is then filtered out into collection.
(3) 92 DEG C of hot water is added in upper step process container until the gross mass of surplus material and water is 1000kg, guarantor
92 ± 2 DEG C of water temperature is held, extracts 3 hours, leaching liquor L2 is then filtered out into collection.
(4) 85 DEG C of hot water is added in upper step process container until the gross mass of surplus material and water is 1100kg, adds
Enter salt 0.8kg, stir evenly, keep 85 ± 2 DEG C of water temperature, extracts 3 hours, leaching liquor L3 is then filtered out into collection.
(5) 80 DEG C of hot water is added in upper step process container until the gross mass of surplus material and water is 800kg, adds
Enter the acetic acid 4kg of 10% concentration, the ethyl alcohol 10kg of 75% alcoholic strength is added, keep 80 ± 2 DEG C of water temperature, extract 3 hours, then
Leaching liquor L4 is filtered out into collection.
(6) all filtrate L1, L2, L3, L4 are collected and is mixed, using the production of Wenzhou Chao Yu mechanical equipment Co., Ltd
Mixed liquor is concentrated into 42 baume by 2000L economic benefits and social benefits outer circulation concentrator, point 3 batches.
(7) Inonotus obliquus crude extract is made in sterile filling.
Embodiment 2:
A kind of preparation method of Inonotus obliquus crude extract:
(1) graphene of the northeast 500kg Inonotus obliquus and 30kg are put into container under the conditions of 4 DEG C and are crushed to 20 mesh,
And use ultraviolet treatment with irradiation 1.5h；
(2) stop ultraviolet treatment with irradiation, above-mentioned Inonotus obliquus is placed in the container that inner wall is nonmetallic materials, is added
Enter normal-temperature water 2500kg, extracts 3 hours, leaching liquor L1 is then filtered out into collection.
(3) 85 DEG C of hot water is added in upper step process container until the gross mass of surplus material and water is 2500kg, guarantor
85 ± 2 DEG C of water temperature is held, extracts 2 hours, leaching liquor L2 is then filtered out into collection.
(4) 75 DEG C of hot water is added in upper step process container until the gross mass of surplus material and water is 2500kg, adds
Enter salt 0.5kg, stir evenly, keep 75 ± 2 DEG C of water temperature, extracts 2 hours, leaching liquor L3 is then filtered out into collection.
(5) 70 DEG C of hot water is added in upper step process container until the gross mass of surplus material and water is 2000kg, adds
Enter the acetic acid 5kg of 5% concentration, the ethyl alcohol 20kg of 50% alcoholic strength is added, keep 70 ± 2 DEG C of water temperature, extract 2 hours, then
Leaching liquor L4 is filtered out into collection.
(6) all filtrate L1, L2, L3, L4 are collected and is mixed, using the production of Wenzhou Chao Yu mechanical equipment Co., Ltd
Mixed liquor is concentrated into 39 baume by 2000L economic benefits and social benefits outer circulation concentrator, point 6 batches.
(7) Inonotus obliquus crude extract is made in sterile filling.
Embodiment 3:
A kind of preparation method of Inonotus obliquus crude extract:
(1) graphene of 200kg Russia Inonotus obliquus and 10kg are put into container under the conditions of 1 DEG C and are crushed to 50
Mesh, and use ultraviolet treatment with irradiation 1h；
(2) stop ultraviolet treatment with irradiation, above-mentioned Inonotus obliquus is placed in the container that inner wall is nonmetallic materials, is added
Enter normal-temperature water 1000kg, extract 2 hours, using ultrasonic treatment 0.5h, leaching liquor L1 is then filtered out into collection.
(3) 92 DEG C of hot water is added in upper step process container until the gross mass of surplus material and water is 1000kg, guarantor
92 ± 2 DEG C of water temperature is held, is extracted 3 hours, using ultrasonic treatment 0.3h, leaching liquor L2 is then filtered out into collection.
(4) 85 DEG C of hot water is added in upper step process container until the gross mass of surplus material and water is 1100kg, adds
Enter salt 0.8kg, stir evenly, keep 85 ± 2 DEG C of water temperature, extract 3 hours, using ultrasonic treatment 0.2h, then by leaching liquor L3
Filter out collection.
(5) 80 DEG C of hot water is added in upper step process container until the gross mass of surplus material and water is 800kg, adds
Enter the acetic acid 4kg of 10% concentration, the ethyl alcohol 10kg of 75% alcoholic strength is added, keep 80 ± 2 DEG C of water temperature, extract 3 hours, uses
It is ultrasonically treated 0.2h, leaching liquor L4 is then filtered out into collection.
(6) all filtrate L1, L2, L3, L4 are collected and is mixed, using the production of Wenzhou Chao Yu mechanical equipment Co., Ltd
Mixed liquor is concentrated into 42 baume by 2000L economic benefits and social benefits outer circulation concentrator, point 3 batches.
(7) Inonotus obliquus crude extract is made in sterile filling.
Test one,
With reference to the method that " research of Inonotus obliquus liquid fermentation and polysaccharide extracting process " provides, it is bent to carry out Glucose standards
The drafting of line.Using phend-sulphuric acid: taking 0.1g polysaccharide semifinished product deionized water dissolving and be settled to mL.Take 0.1mL sample dilute
It takes 0.5mL in test tube after 10 times, 6% phenol 1mL is added, the concentrated sulfuric acid is settled to 10mL, and 80 DEG C of thermostatted waters are put into after shaking up
Water-bath 20mi n in bath.OD value at 490nm is measured, repeats, is averaged three times.
It is calculated using the 1/100 of Inonotus obliquus material quality as Inonotus obliquus dry weight, by various embodiments of the present invention preparation
The 1/100 of Inonotus obliquus crude extract total amount, and in paper " research of Inonotus obliquus liquid fermentation and polysaccharide extracting process "
It is comparative example that prior art extraction process, which makees the extracted Inonotus obliquus crude extract of extraction process, under the conditions of identical temperature,
The absolute alcohol of 3 times of volumes of crude extract is added, stands 12h.Centrifugation is dried after collecting precipitating to get Thick many candies dry weight.
Embodiment and comparative example is weighed to 0.05g Thick many candies dry weight sample constant volume in 25mL volumetric flask, draws 1.0mL
Phend-sulphuric acid measures OD value at 490nm, brings glucose standard curve into and converts to obtain Fuscoporia obliqua polysaccharide content.
Wherein, coarse polysaccharide extractive rate (%)=[Thick many candies dry weight (g)/Inonotus obliquus dry weight (g)] × 100%；
The relative amount (%) of Fuscoporia obliqua polysaccharide=[amount (g) of Fuscoporia obliqua polysaccharide/Thick many candies amount (g)] ×
100%, wherein the amount of Thick many candies is 0.05g.
It is existing in the embodiment of the present invention 1~3 and paper " research of Inonotus obliquus liquid fermentation and polysaccharide extracting process "
Technology extraction process as a comparison case, to the Inonotus obliquus crude extract of extraction according to above-mentioned test method and calculation, obtains
The results are shown in Table 1:
Table 1
It can be seen from Table 1 that birch prepared by the preparation method of Inonotus obliquus crude extract provided in an embodiment of the present invention is brown
The extraction process institute that the coarse polysaccharide extractive rate of pore fungi crude extract and the relative amount of Fuscoporia obliqua polysaccharide are provided compared with comparative example
The Inonotus obliquus crude extract of extraction is significantly increased.Further, embodiment 3 uses the processing mode of ultrasound, the brown hole of birch
The relative amount of granulose further increases compared with embodiment 1 and embodiment 2.
Test two,
The Inonotus obliquus crude extract that the embodiment of the present invention of same volume and comparative example are extracted, in 28 DEG C of ± 1 DEG C of decentralizations
Enter in identical Potato-dextrose-agar medium, and culture medium be placed in gnotobasis and is stood, maintains the circulation of air,
Fungi count (CFU/g) in 7 days, 14 days and 21 days observation culture mediums respectively；Wherein one group of culture medium is added without Inonotus obliquus
Crude extract, it is other consistent, blank is carried out referring to experiment.Test result is as shown in table 2:
Table 2
| Time | Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 | Blank control |
| 7 days | 7CFU/g | 6CFU/g | 8CFU/g | 19CFU/g | 3CFU/g |
| 14 days | 12CFU/g | 13CFU/g | 12CFU/g | 67CFU/g | 9CFU/g |
| 21 days | 23CFU/g | 22CFU/g | 25CFU/g | 98CFU/g | 13CFU/g |
It can be seen from Table 2 that birch prepared by the preparation method of Inonotus obliquus crude extract provided in an embodiment of the present invention
Inonotus obliquus crude extract prepared by the preparation process that brown pore fungi crude extract provides comparative example is compared, and mould bacterial content is few, therefore
It can save more for a long time, there are significant progress and huge market prospects compared with prior art.
Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations；To the greatest extent
Pipe present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that: its according to
So be possible to modify the technical solutions described in the foregoing embodiments, or to some or all of the technical features into
Row equivalent replacement；And these are modified or replaceed, various embodiments of the present invention technology that it does not separate the essence of the corresponding technical solution
The range of scheme.
Claims (10)
1. a kind of preparation method of Inonotus obliquus crude extract, it is characterised in that: including following preparation step:
S100, Inonotus obliquus raw material and graphene are put into container；
S200, in container Inonotus obliquus raw material and graphene carry out break process formed mixture M 1；
S300, processing is irradiated to the M1 in container using ultraviolet light；
S400, after stopping UV treatment, extraction processing is carried out to mixture M 1, obtains leaching liquor L；
Specifically, extraction temperature is 20 DEG C~95 DEG C, and extraction time is 6~18 hours；
S500, obtained leaching liquor L is subjected to concentration, that is, Inonotus obliquus crude extract is prepared.
2. a kind of preparation method of Inonotus obliquus crude extract according to claim 1, it is characterised in that: in step S400,
Extraction processing the following steps are included:
S410, the normal-temperature water that 20 DEG C~30 DEG C are added into container, carry out extraction processing, leaching liquor L1 are obtained after filtering, remaining
Solid mixture M2；
S420, into container in remaining solid mixing M2,70 DEG C~95 DEG C of hot water is added, extraction processing is carried out, after filtering
Obtain leaching liquor L2, surplus solid mixture M3；
S430, into container in remaining solid mixing M3, inorganic solution is added, carries out extraction processing, is extracted after filtering
Liquid L3, surplus solid mixture M4；
S440, into container in remaining solid mixing M4, organic solution is added, carries out extraction processing, is extracted after filtering
Liquid L4, surplus solid mixture M5；
In S500 concentration, leaching liquor L1, L2, L3, L4 are mixed, concentration is carried out, that is, it is thick that Inonotus obliquus is prepared
Extract.
3. a kind of preparation method of Inonotus obliquus crude extract according to claim 1, it is characterised in that: in step S200,
Mechanical Crushing processing is carried out in 0~4 DEG C of environment, is crushed to 20~60 mesh.
4. a kind of preparation method of Inonotus obliquus crude extract according to claim 2, it is characterised in that: in step S410,
When using room temperature flooding, the feed liquid mass ratio of Inonotus obliquus raw material dry material quality and normal-temperature water is 1:3~1:8, when extraction
Between be 2~5 hours.
5. a kind of preparation method of Inonotus obliquus crude extract according to claim 2, it is characterised in that: in step S420,
When using hot water extraction, the feed liquid mass ratio of Inonotus obliquus raw material dry material quality and hot water is 1:5~1:10, and extraction time is
1~5 hour.
6. a kind of preparation method of Inonotus obliquus crude extract according to claim 2, it is characterised in that: in step S430,
When being extracted using inorganic solution, inorganic solution is salt solution；Wherein salt quality is Inonotus obliquus raw material dry material quality
1 ‰~5 ‰, the feed liquid mass ratio of Inonotus obliquus raw material dry material quality and salt solution is 1:4~1:7, extraction time is 2~
4 hours.
7. a kind of preparation method of Inonotus obliquus crude extract according to claim 2, it is characterised in that: in step S440,
When being extracted using organic solution, organic solution is colourless acetic acid and the brown hole of birch of Inonotus obliquus raw material dry material quality 1%~3%
The ethyl alcohol of bacterium raw material dry material quality 3%~11%, extraction time are 1~4 hour；
Wherein, the solubility of the colourless acetic acid is 5%~10%；The concentration of ethyl alcohol is 50%~75%.
8. according to claim 1 or a kind of 2 described in any item preparation methods of Inonotus obliquus crude extract, it is characterised in that: institute
The inner wall for stating container is made of nonmetallic materials.
9. according to claim 1 or a kind of 2 described in any item preparation methods of Inonotus obliquus crude extract, it is characterised in that: institute
It states concentration and vacuum concentration processing, concentration to 38~45 Baume degrees is carried out using economic benefits and social benefits falling film type concentrator.
10. a kind of mentioned using Inonotus obliquus prepared by the preparation method of Inonotus obliquus crude extract a kind of as described in 1~7 any one
Take liquid.
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| CN104004106A (en) * | 2014-04-28 | 2014-08-27 | 杨庆贺 | Method for extracting effective substances in inonotus obliquus and preparation method for teabag |
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| CN104004106A (en) * | 2014-04-28 | 2014-08-27 | 杨庆贺 | Method for extracting effective substances in inonotus obliquus and preparation method for teabag |
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