CN107468549A - A kind of bitter oily veepa oil nano-composition and its preparation method and application - Google Patents
A kind of bitter oily veepa oil nano-composition and its preparation method and application Download PDFInfo
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- CN107468549A CN107468549A CN201710949617.1A CN201710949617A CN107468549A CN 107468549 A CN107468549 A CN 107468549A CN 201710949617 A CN201710949617 A CN 201710949617A CN 107468549 A CN107468549 A CN 107468549A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/04—Dispersions; Emulsions
- A61K8/06—Emulsions
- A61K8/068—Microemulsions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/02—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings containing insect repellants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/41—Particular ingredients further characterized by their size
- A61K2800/413—Nanosized, i.e. having sizes below 100 nm
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/70—Biological properties of the composition as a whole
- A61K2800/72—Hypo-allergenic
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Abstract
The invention provides a kind of bitter oily veepa oil nano-composition, belong to cosmetic field, include the component of following weight percentage：Bitter oily veepa oil 1.0%~30.0%, emulsifying agent 25.0%~75.0%, assistant for emulsifying agent 0.8%~17.0%, polyalcohol 1.0%~65.0%, antioxidant 0.01%~2.0%, preservative 0.01%~4.0%.The present invention forms thermodynamically stable homogeneous dispersion system with bitter oily veepa oil, emulsifying agent, assistant for emulsifying agent, polyalcohol, and good water solubility, stability is high, without oil slick, clustering phenomena, has good mosquito repellent, antibacterial, anti-inflammatory, analgesic effect.The oily veepa oil nano-composition drugloading rate of hardship provided by the invention is big, and bitter oily veepa oil nano-composition can pass through keratoderma and enter deep skin tissue, the bitter oily veepa oil of sustained release, preferably play mosquito repellent, antibacterial, anti-inflammatory, analgesia and other effects.
Description
Technical field
The present invention relates to cosmetic field, more particularly to a kind of bitter oily veepa oil nano-composition and preparation method thereof and should
With.
Background technology
Bitter oily chinaberry is a kind of high seeds common in tropical rain forest, can grow to 40 meters of height, and production one kind is similar to chestnut on tree
The brown square nut of son, it is about 3~4 inches wide.Bitter oily veepa oil is squeezed by the kernel of this nut and obtain, with anti-inflammatory,
Antibacterial, antirheumatic arthritis, mosquito repellent and other effects.
Bitter oily veepa oil is mainly made up of oleic acid, palmitic acid, stearic acid, linoleic acid etc., has oxidizable rotten, stability
The shortcomings that difference, difficulty are scattered in water, causes its bioavailability low, it is difficult to be directly appended to cosmetics, health products, medicine
In.Therefore the dissolubility and storage stability of bitter oily veepa oil in an aqueous medium how are improved, turns into it and is producing and using
During key technical problem.
The content of the invention
In view of this, it is an object of the invention to provide a kind of bitter oily veepa oil nano-composition, bitter oily veepa oil is improved
The water solubility and stability of nano-composition.
In order to realize foregoing invention purpose, the present invention provides following technical scheme：
The invention provides a kind of bitter oily veepa oil nano-composition, include the component of following weight percentage：
Bitter oily veepa oil 1.0%~30.0%, emulsifying agent 25.0%~75.0%, assistant for emulsifying agent 0.8%~17.0% are more
First alcohol 1.0%~65.0%, antioxidant 0.01%~2.0%, preservative 0.01%~4.0%.
Preferably, the oily veepa oil nano-composition of the hardship includes the component of following weight percentage：
Bitter oily veepa oil 5.0%~25.0%, emulsifying agent 30.0%~65.0%, assistant for emulsifying agent 2.0%~15.0% are more
First alcohol 5.0%~55.0%, antioxidant 0.05%~1.5%, preservative 0.05%~4.0%.
Preferably, the particle diameter of the oily veepa oil nano-composition of the hardship is 20~150nm.
Preferably, the emulsifying agent includes poly yamanashi esters emulsifying agent, Emulsifier EL-60 class emulsifying agent, polyethylene glycol
One or more of mixtures in fatty acid ester emulsifying agent and ceteareth class emulsifying agent.
Preferably, the assistant for emulsifying agent is to include TC, butylol polyether -9, butylol polyether -12, butanol
One or more of mixtures in polyethers -26, butylol polyether -35, PPG-26- butylol polyethers -26 and poloxamer.
Preferably, the polyalcohol includes polyethylene glycol, propane diols, DPG, glycerine, 1,2- hexylene glycols, 1,3- fourths
One or more of mixtures in glycol, 1,2- pentanediols, octyl dodecanol, sorbierite and pungent glycol.
Preferably, the antioxidant includes vitamin C, VCE, vitamin E, Vitwas E, not had
In gallate-based, butylated hydroxy anisole, dibutyl hydroxy toluene, tert-butylhydroquinone, phytic acid, Rosmarinic acid and Tea Polyphenols
One or more of mixtures.
Preferably, the preservative includes Phenoxyethanol, methylisothiazolinone, potassium sorbate, DMDMH, miaow
One or more of mixtures in oxazolidinyl urea and Sensiva SC50.
Present invention also offers the preparation method of bitter oily veepa oil nano-composition described in above-mentioned technical proposal, including it is following
Step：
Emulsifying agent, assistant for emulsifying agent, polyalcohol, antioxidant and preservative are mixed, obtain just batch mixing；
The just batch mixing is mixed with bitter oily veepa oil, obtains bitter oily veepa oil nano-composition.
Present invention also offers bitter oily veepa oil nano-composition or above-mentioned preparation method described in above-mentioned technical proposal to obtain
Application of the bitter oily veepa oil nano-composition in cosmetics.
The invention provides a kind of bitter oily veepa oil nano-composition, include the component of following weight percentage：Bitter oil
Veepa oil 1.0%~30.0%, emulsifying agent 25.0%~75.0%, assistant for emulsifying agent 0.8%~17.0%, polyalcohol 1.0%~
65.0%, antioxidant 0.01%~2.0%, preservative 0.01%~4.0%.The bitter oily veepa oil of the present invention, emulsifying agent, help
Emulsifying agent, polyalcohol form thermodynamically stable homogeneous dispersion system, and good water solubility, stability is high, existing without oil slick, aggregation
As having good mosquito repellent, antibacterial, anti-inflammatory, analgesic effect.The oily veepa oil nano-composition of hardship provided by the invention realizes
Larger drugloading rate, the mass concentration of the bitter oily veepa oil of active component is up to 30.0%；Bitter oily veepa oil nano-composition can be saturating
Cross keratoderma and enter deep skin tissue, the bitter oily veepa oil of sustained release, maintain valid density for a long time, preferably send out
Wave mosquito repellent, antibacterial, anti-inflammatory, analgesia and other effects.Stability experiment result shows that the oily veepa oil nanometer of hardship provided by the invention combines
After thing is placed 30 days, conspicuousness change does not occur for character and particle diameter.Mosquito repellent experiment, bacteriostatic experiment, the result of anti-inflammatory analgesic experiment
Show, the oily veepa oil nano-composition of hardship provided by the invention has the effect of very significant mosquito repellent, antibacterial, anti-inflammatory, analgesia.
Therefore, the oily veepa oil nano-composition stability of hardship provided by the invention is high, drugloading rate is big, skin irritation is small, biologically effective
Availability is high, can be widely used in cosmetic field.
Also, the preparation method of the oily veepa oil nano-composition of hardship provided by the invention is simple, chemical change does not occur, no
Change the chemical constitution of bitter oily veepa oil, technique is easily controllable, suitable industrialization, large-scale production, and does not make in whole process
It is safe with organic solvent, green, low energy consumption.
Embodiment
The invention provides a kind of bitter oily veepa oil nano-composition, include the component of following weight percentage：
Bitter oily veepa oil 1.0%~30.0%, emulsifying agent 25.0%~75.0%, assistant for emulsifying agent 0.8%~17.0% are more
First alcohol 1.0%~65.0%, antioxidant 0.01%~2.0%, preservative 0.01%~4.0%.
The oily veepa oil nano-composition of hardship provided by the invention includes the bitter oily chinaberry of 1.0%~30.0% weight percentage
Tree oil, preferably 5.0%~25.0%, more preferably 10.0%~20.0%.In the present invention, the oily veepa oil of hardship is rich in people
Aliphatic acid necessary to body, such as oleic acid, palmitic acid, stearic acid and linoleic acid, also containing natural limonin, act not only as
A kind of NMF assists in keeping the integrality of epidermal cell structure, additionally it is possible to and bacterium is effectively antagonized, promotes skin renewal,
Accelerate wound healing, reduce the inflammation of skin and muscle.The present invention is not limited the source of the oily veepa oil of hardship, using city
Sell bitter oily veepa oil commodity.
The oily veepa oil nano-composition of hardship provided by the invention includes the emulsification of 25.0%~75.0% weight percentage
Agent, preferably 30.0%~65.0%, more preferably 45.0%~60.0%.In the present invention, the emulsifying agent preferably includes
Poly yamanashi esters emulsifying agent, Emulsifier EL-60 class emulsifying agent, cithrol class emulsifying agent and cetostearyl alcohol
One or more of mixtures in polyethers emulsifying agent, the mixture of more preferably 2~4 kinds emulsifying agents, most preferably 2~3
The mixture of kind emulsifying agent.In the present invention, the poly yamanashi esters emulsifying agent preferably includes Tween 80, polysorbate60, polysorbate40
With the one or more in polysorbas20.In the present invention, the Emulsifier EL-60 class emulsifying agent preferably includes polyoxyethylene
Castor oil-20, Emulsifier EL-60-30, Emulsifier EL-60-35, Emulsifier EL-60-40, PEG40 hydrogenated castors
One or more in oil, PEG30 rilanit specials, PEG20 rilanit specials and PEG10 rilanit specials.In the present invention,
The cithrol class emulsifying agent preferably includes the double stearic acid of PEG400MS, polyethylene glycol 400
One or more in ester and polyethylene glycol 400 dioleic acid ester.In the present invention, the ceteareth class emulsifying agent is excellent
Choosing includes ceteareth -20 and/or ceteareth -25.In embodiments of the present invention, the emulsifying agent is preferred
For Tween 80 and the mixture of Emulsifier EL-60 ether -40, or Emulsifier EL-60 ether -40, PEG10 rilanit specials
With the mixture of ceteareth -25.The present invention is not limited the mass ratio of various emulsifying agents in mixture, this area
Technical staff can be according to the mixture for being actually needed the emulsifying agent using any mass ratio.
The oily veepa oil nano-composition of hardship provided by the invention helps emulsification including 0.8%~17.0% weight percentage
Agent, preferably 2.0%~15.0%, more preferably 5.0%~12.0%.In the present invention, the assistant for emulsifying agent preferably includes
TC, butylol polyether -9, butylol polyether -12, butylol polyether -26, butylol polyether -35, PPG-26- butanol gather
One or more of mixtures in ether -26 and poloxamer, the mixture of more preferably 2~3 kinds assistant for emulsifying agents, it is most preferably
The mixture of TC and butylol polyether -26 and PPG-26- butylol polyethers -26.The present invention is to the mixture
In the mass ratio of each assistant for emulsifying agent do not limit, those skilled in the art can be according to being actually needed helping using any mass ratio
The mixture of emulsifying agent.In the present invention, the emulsifying agent mutually acts synergistically with assistant for emulsifying agent, plays stabilizing actives hardship
The effect of oily veepa oil so that the bitter oily veepa oil nano-composition drugloading rate of offer is big, can contain more active materials, group
For the mass concentration of the bitter oily veepa oil of active component up to 30.0%, bitter oily veepa oil nano-composition, which is used as, passes through skin in compound
Bank, the sustainable bitter oily veepa oil of release, valid density can be maintained for a long time, mosquito repellent, antibacterial is preferably played, disappear
Scorching, analgesia and other effects.
The oily veepa oil nano-composition of hardship provided by the invention includes the polynary of 1.0%~65.0% weight percentage
Alcohol, preferably 5.0%~55.0%, more preferably 10.0%~50.0%.In the present invention, the polyalcohol preferably includes to gather
Ethylene glycol, propane diols, DPG, glycerine, 1,2- hexylene glycols, 1,3 butylene glycol, 1,2- pentanediols, octyl dodecanol, sorb
One or more of mixtures in alcohol and pungent glycol, the most preferably mixture of more preferably 2~3 kinds polyalcohols, propane diols
With glycerine and 1,2- hexylene glycols, 1,3 butylene glycol and the mixture of 1,2- pentanediols and sorbierite.The present invention is to the mixture
In the mass ratio of each polyalcohol do not limit, those skilled in the art can be according to being actually needed using the polynary of any mass ratio
The mixture of alcohol.In the present invention, the polyalcohol can strengthen the dissolubility of bitter oily veepa oil nano-composition, further carry
The stability of high bitter oily veepa oil nano-composition.
The oily veepa oil nano-composition of hardship provided by the invention includes the anti-oxidant of 0.01%~2.0% weight percentage
Agent, preferably 0.05%~1.5%, more preferably 0.1%~1.0%.In the present invention, the antioxidant preferably includes to tie up
Raw plain C, VCE, vitamin E, Vitwas E, gallic acid, butylated hydroxy anisole, dibutyl hydroxy
One or more of mixtures in toluene, tert-butylhydroquinone, phytic acid, Rosmarinic acid and Tea Polyphenols, more preferably 2~3
The mixture of kind antioxidant, most preferably gallic acid and butylated hydroxy anisole, dibutyl hydroxy toluene and tertiary butyl pair
The mixture of benzenediol and Rosmarinic acid.The present invention is not limited the mass ratio of each antioxidant in the mixture, ability
Field technique personnel can be according to the mixture for being actually needed the antioxidant using any mass ratio.In the present invention, it is described anti-
Oxidant can prevent bitter oily veepa oil nano-composition to be oxidized, and further improve the stabilization of bitter oily veepa oil nano-composition
Property.
The oily veepa oil nano-composition of hardship provided by the invention includes the anti-corrosion of 0.01%~4.0% weight percentage
Agent, preferably 0.05%~4.0%, more preferably 0.1%~2.0%.In the present invention, the preservative preferably includes benzene oxygen
One kind in ethanol, methylisothiazolinone, potassium sorbate, DMDMH, imidazolidinyl urea and Sensiva SC50 or
Several mixtures, the mixture of more preferably 2 kinds preservatives, most preferably Phenoxyethanol and potassium sorbate, ethylhexyl are sweet
The mixture of oil and DMDMH.The present invention is not limited the mass ratio of each preservative in the mixture, this area
Technical staff can be according to the mixture for being actually needed the preservative using any mass ratio.In the present invention, the preservative
It can prevent that bitter oily veepa oil nano-composition is putrid and deteriorated, further improve the stability of bitter oily veepa oil nano-composition.
The particle diameter of the oily veepa oil nano-composition of hardship provided by the invention is preferably 20~150nm, more preferably 30~
120nm, most preferably 40~80nm, more preferably 60nm.In the present invention, the hardship oily veepa oil nano-composition
Particle diameter is preferably 20~150nm, good penetrability, is more beneficial for skin absorption.
Present invention also offers the preparation method of bitter oily veepa oil nano-composition described in above-mentioned technical proposal, including it is following
Step：
Emulsifying agent, assistant for emulsifying agent, polyalcohol, antioxidant and preservative are mixed, obtain just batch mixing；
The just batch mixing is mixed with bitter oily veepa oil, obtains bitter oily veepa oil nano-composition.
The present invention mixes emulsifying agent, assistant for emulsifying agent, polyalcohol, antioxidant and preservative, obtains just batch mixing.The present invention
There is no any special restriction to the charging sequence of the emulsifying agent, assistant for emulsifying agent, polyalcohol, antioxidant and preservative, adopt
With charging sequence well known to those skilled in the art, in the present invention, it is preferred to add emulsifying agent, assistant for emulsifying agent, more one by one
First alcohol, antioxidant and preservative.The present invention does not have special restriction to the concrete mode of the mixing, using art technology
Hybrid mode known to personnel, it is specific such as magnetic agitation.In the present invention, the mixing is preferably under conditions of heating
Carry out, the temperature of the heating is preferably 45~75 DEG C, more preferably 50~70 DEG C, most preferably 55~65 DEG C；The mixing
Time be preferably 10~30min, more preferably 12~25min, most preferably 15~20min.In the present invention, it is preferred to using
The mode of jacketed pan heating water bath reaches the temperature of the heating.
After obtaining just batch mixing, the present invention mixes the just batch mixing with bitter oily veepa oil, obtains bitter oily veepa oil nanometer group
Compound.In the present invention, the just batch mixing is preferably 45~75 DEG C, more preferably 50~70 with the temperature that bitter oily veepa oil mixes
DEG C, most preferably 55~65 DEG C；The time of the mixing is preferably 20~60min, more preferably 30~50min, is most preferably
40min.In the present invention, it is preferred to reach the temperature of the mixing by the way of jacketed pan heating water bath.The present invention is to described
The mode of mixing does not have special restriction, using hybrid mode well known to those skilled in the art, in the present invention, it is preferred to
For stirring, the present invention does not have special restriction to the rotating speed of the stirring, and use is well known to those skilled in the art, it is each to make
Raw material is well mixed.
Present invention also offers bitter oily veepa oil nano-composition or above-mentioned technical proposal described in above-mentioned technical proposal are any
Application of the bitter oily veepa oil nano-composition that one preparation method obtains in cosmetics.
In the present invention, mass fraction of the oily veepa oil nano-composition of the hardship in cosmetics is preferably 1~30%,
More preferably 5~20%.
In the present invention, the product form of the oily veepa oil nano-composition of the hardship includes：Creme, aqua, emulsion, spray
Agent, gel.
Oily veepa oil nano-composition of hardship provided by the invention and its preparation method and application is entered with reference to embodiment
Row detailed description, but they can not be interpreted as limiting the scope of the present invention.
Embodiment 1
By 5% Tween 80,15% Emulsifier EL-60 ether -40,3% PEG400MS, 2% spermaceti
Stereth -25,20% propane diols, 40%1,3- butanediols, 5%1,2- pentanediols, 5% TC, 1%
Butylol polyether -9,1% VCE, 1% Vitwas E, 1% Phenoxyethanol under 45 DEG C of water bath conditions with
200rpm rotating speed stirring 10min, after being well mixed, 1% bitter oily veepa oil is added, continuing stirring 20min makes whole system
Transparent and homogeneous, it is cooled to after room temperature and obtains bitter oily veepa oil nano-composition.
The particle diameter of bitter oily veepa oil nano-composition is detected, can obtain the oily veepa oil nano-composition particle diameter of the hardship is
149.9nm。
Embodiment 2
By 20% polysorbas20,12%PEG30 rilanit specials, 3% ceteareth -20,29.5% glycerine, 20%
1,3 butylene glycol, 7% sorbierite, 2% butylol polyether -35,1% vitamin E, 0.5% imidazolidinyl urea are in 50 DEG C of water bath conditions
Under 15min stirred with 400rpm rotating speed, after being well mixed, add 5% bitter oily veepa oil, continuing to stir 30min makes entirely
System transparent and homogeneous, it is cooled to after room temperature and obtains bitter oily veepa oil nano-composition.
The particle diameter of bitter oily veepa oil nano-composition is detected, can obtain the oily veepa oil nano-composition particle diameter of the hardship is
121.6nm。
Embodiment 3
By 12% polysorbate60,18% Emulsifier EL-60-40,15%PEG10 rilanit specials, 5% cetostearyl alcohol
Polyethers -25,15% propane diols, 10% DPG, 5%1,2- hexylene glycols, 2% TC, 3% butanol gather
Ether -26,1% vitamin C, 4% potassium sorbate stir 20min under 60 DEG C of water bath conditions with 500rpm rotating speed, are well mixed
Afterwards, 10% bitter oily veepa oil is added, continuing stirring 40min makes whole system transparent and homogeneous, and hardship is obtained after being cooled to room temperature
Oily veepa oil nano-composition.
The particle diameter of bitter oily veepa oil nano-composition is detected, can obtain the oily veepa oil nano-composition particle diameter of the hardship is
80.3nm。
Embodiment 4
30% Emulsifier EL-60-20,20%PEG20 rilanit specials, 10%PEG10 rilanit specials, 5% are gathered
400 pairs of stearates of ethylene glycol, 5%1,2- hexylene glycols, 2% octyl dodecanol, 4% sorbierite, 3% diethylene glycol mono-ethyl
Ether, 3% butylol polyether -12,1% poloxamer, 1% gallic acid, 1% Phenoxyethanol are under 65 DEG C of water bath conditions with 600rpm
Rotating speed stirring 20min, after being well mixed, add 15% bitter oily veepa oil, continuing to stir 45min makes whole system homogeneous
It is bright, it is cooled to after room temperature and obtains bitter oily veepa oil nano-composition.
The particle diameter of bitter oily veepa oil nano-composition is detected, can obtain the oily veepa oil nano-composition particle diameter of the hardship is
56.7nm。
Embodiment 5
By 40% Tween 80,20%PEG40 rilanit specials, 10%PEG20 rilanit specials, 4.5% polyethylene glycol 400
Dioleic acid ester, 1% pungent glycol, 3.98%PPG-26- butylol polyethers -26,0.02% butylated hydroxy anisole, 0.5%DMDM second
Interior uride stirs 25min under 70 DEG C of water bath conditions with 700rpm rotating speed, after being well mixed, adds 20% bitter oily chinaberry
Oil, continuing stirring 50min makes whole system transparent and homogeneous, is cooled to after room temperature and obtains bitter oily veepa oil nano-composition.
The particle diameter of bitter oily veepa oil nano-composition is detected, can obtain the oily veepa oil nano-composition particle diameter of the hardship is
21.0nm。
Embodiment 6
By 20% polysorbate40,30% Emulsifier EL-60-30,10%PEG30 rilanit specials, 4%1,2- pentanediols,
4.99%PPG-26- butylol polyethers -26,0.99% poloxamer, 0.01% tert-butylhydroquinone, 0.01% methyl-isothiazol
Quinoline ketone stirs 30min under 75 DEG C of water bath conditions with 800rpm rotating speed, after being well mixed, adds 30% bitter oily veepa oil,
Continuing stirring 60min makes whole system transparent and homogeneous, is cooled to after room temperature and obtains bitter oily veepa oil nano-composition.
The particle diameter of bitter oily veepa oil nano-composition is detected, can obtain the oily veepa oil nano-composition particle diameter of the hardship is
76.8nm。
Embodiment 7
By 10% polysorbate60,35%PEG40 rilanit specials, 4% polyethylene glycol 400 dioleic acid ester, 1% cetostearyl alcohol
Polyethers -20,6.5% glycerine, 5% TC, 10% butylol polyether -26,2%PPG-26- butylol polyethers -26,
0.1% vitamin C, 0.1% vitamin E, 0.3% dibutyl hydroxy toluene, 0.7% Phenoxyethanol, 0.3% Sensiva SC50
20min is stirred with 500rpm rotating speed under 60 DEG C of water bath conditions, after being well mixed, 25% bitter oily veepa oil is added, continues
Stirring 40min makes whole system transparent and homogeneous, is cooled to after room temperature and obtains bitter oily veepa oil nano-composition.
The particle diameter of bitter oily veepa oil nano-composition is detected, can obtain the oily veepa oil nano-composition particle diameter of the hardship is
65.6nm。
Embodiment 8
By 10% Tween 80,10% polysorbas20,35% Emulsifier EL-60-35,1.9% DPG, 12% octyl group
Lauryl alcohol, 3% butylol polyether -9,0.1% Rosmarinic acid, 3% potassium sorbate are under 60 DEG C of water bath conditions with 500rpm rotating speed
20min is stirred, after being well mixed, adds 25% bitter oily veepa oil, continuing stirring 40min makes whole system transparent and homogeneous, cold
But to obtaining bitter oily veepa oil nano-composition after room temperature.
The particle diameter of bitter oily veepa oil nano-composition is detected, can obtain the oily veepa oil nano-composition particle diameter of the hardship is
50.4nm。
Embodiment 9
By 20% polysorbate40,15% Emulsifier EL-60-35,25%PEG30 rilanit specials, 10%1,3- fourths two
Alcohol, 3% sorbierite, 0.8% TC, 0.2% phytic acid, 1% Phenoxyethanol under 60 DEG C of water bath conditions with
500rpm rotating speed stirring 20min, after being well mixed, 25% bitter oily veepa oil is added, continuing stirring 40min makes whole system
Transparent and homogeneous, it is cooled to after room temperature and obtains bitter oily veepa oil nano-composition.
The particle diameter of bitter oily veepa oil nano-composition is detected, can obtain the oily veepa oil nano-composition particle diameter of the hardship is
47.3nm。
Embodiment 10
By 50%PEG40 rilanit specials, 2% PEG400MS, 3% ceteareth -25,
8% polyethylene glycol, 5% glycerine, 2% TC, 3.1% butylol polyether -26,0.5% vitamin E, 0.4% tea
Polyphenol, 0.5% Phenoxyethanol, 0.5% Sensiva SC50 stir 20min under 60 DEG C of water bath conditions with 500rpm rotating speed,
After well mixed, 25% bitter oily veepa oil is added, continuing stirring 40min makes whole system transparent and homogeneous, after being cooled to room temperature
Obtain bitter oily veepa oil nano-composition；
The particle diameter of bitter oily veepa oil nano-composition is detected, can obtain the oily veepa oil nano-composition particle diameter of the hardship is
53.8nm。
Embodiment 11
The bitter oily veepa oil nano-composition that embodiment 1~10 is obtained is placed 30 days under closed container, room temperature condition,
The character and particle diameter of inspection sample, experimental result are as shown in table 1.
The bitter oily veepa oil nano-composition stability test result of 1 embodiment of table 1~10
Stability test result shows：The oily veepa oil nano-composition of hardship provided by the invention in soilless sticking, lamination,
And particle diameter meets application request between 20~150nm, sample does not occur reunion lamination after placing 30 days yet,
Conspicuousness change does not occur for sample particle diameter, still meets practical application request, and system is still especially in the case of drug concentration height
Right transparent and homogeneous, oil slick, clustering phenomena are not found, therefore, the oily veepa oil nano-composition of hardship provided by the invention has good
Stability.
Comparative example 1
Bitter oily veepa oil creme is prepared according to conventional creme preparation method：By 2.5% bitter oily veepa oil, 3.0% stearic acid,
4.5% glycerin monostearate, 5.0% cetostearyl alcohol, 6.0% dimethyl silicone polymer melt in 75 DEG C of water-baths, obtain
Oil phase；10.0% glycerine, 5.0% propane diols, 0.3% triethanolamine and 63.7% purified water are dissolved in 75 DEG C of water-baths, obtained
To aqueous phase；Oil phase and aqueous phase are stirred, and emulsified, is cooled down, produces the bitter oily veepa oil creme that content is 2.5%.
Comparative example 2
The blank creme for not containing bitter oily veepa oil is prepared by the formula in comparative example 1 and method, is prepared with embodiment 2
The bitter oily veepa oil nano-composition that bitter oily chinaberry oil concentration is 5.0%, according to mass ratio 1:1 is compounded, and obtains containing 2.5%
Bitter oily veepa oil nano-composition creme, as creme compare.
Embodiment 12
Example 2~5 prepare bitter oily veepa oil nano-composition sample, respectively with the blank creme in comparative example 1
According to mass ratio 1:1 is compounded, and carries out skin irritation test：
Healthy rabbits 18 are taken, body weight (2.0 ± 0.2) kg, are randomly divided into 6 groups, every group of animal 3,24h will before experiment
Rabbit skin of back both sides unhairing, 24h checks whether skin of unhairing is injured after unhairing, and compromised skin is unfit to do skin irritation examination
Test.The creme 3 times containing bitter oily veepa oil nano-composition prepared using embodiment 2~5 is smeared daily, it is continuous to smear 7 days,
Smear blank creme (not giving any medicine) simultaneously to be compareed, viewing test result, result of the test is listed in Table 2 below.
Result of the test in table 2 can be seen that contains bitter oily veepa oil nanometer group using prepared by embodiment 2~5
The creme and blank creme of compound are applied to after a rabbit skin without congested, red and swollen phenomenon, illustrate the oily chinaberry of hardship provided by the invention
Set oily nano-composition does not have excitant to skin.
The nano-composition of 2 embodiment of table 2~5 and blank group skin irritation observation result
"+" rabbit dermohemia, redness；" ++ " represents that congested, red and swollen phenomenon still exists, but has increase trend；"-" represents
Without congested, red and swollen phenomenon.
Embodiment 13
600 aedes albopictus pupas are put into 20cm × 20cm × 30cm mosquito cages, are driven after its emergence in 5~10d
Mosquito is tested, and investigates comparative example 1, comparative example 2, embodiment 2, the repelling effect of embodiment 3.25~27 DEG C of laboratory temperature, relatively
Humidity 60%~70%.
In each exposed 5cm × 6cm of the both hands back of attack pass the test person skin, remainder tightly covers.Its
In 1 hand uniformly smear 1.5mg/cm as blank control, another the back of the hand exposed skin2Test specimen, hand is stretched after 5min
Enter and tested in the qualified mosquito cage of attack, observe and the mosquito thorn that comes to dwell on is whether there is in 2min sting and record the mosquito time of dwelling on.It is later every
30min is tested 1 time.Experiment 2min mosquitoes thorn of dwelling on stings mosquito time every time in record 2h, 4h, 6h, 12h.Examination is repeated 3 times per middle sample
Test, effect examination stings number according to thorn of dwelling on and calculates Effective Vate of Protection, and its formula is as follows：
Result of the test in table 3, which can be seen that comparative example 1, has repelling effect, but the repellent time is not lasting, and right
The Effective Vate of Protection of ratio 2, embodiment 2, embodiment 3 within 12h is more than 95%.Illustrate the oily chinaberry of hardship provided by the invention
Setting oily nano-composition has good repelling effect, and extends repellent time of the bitter oily veepa oil to mosquito.
The Effective Vate of Protection of 3 different samples of table compares
Embodiment 14
Comparative selection example 1, comparative example 2, embodiment 2, embodiment 3 make Antimicrobial test.
Strain：Staphylococcus aureus, Escherichia coli, Candida albicans.
The preparation of bacterium solution：Test strain is inoculated in plain agar flat board 37 DEG C 18 hours, culture is matched somebody with somebody with physiological saline
Into bacteria suspension, judge bacterium amount through turbidimetry, prepare two kinds of bacteria concentrations：(1) every milliliter of turbid concentration bacterium equivalent to 900,000,000 bacteriums
Liquid；(2) every milliliter of turbid concentration bacterium solution equivalent to 300,000,000 bacteriums.
The preparation of sample solution：Comparative example 1, comparative example 2, embodiment 2, embodiment 3 are diluted 10 times with ultra-pure water, are made
Concentration is 10% sample solution.
The measure of fungistatic effect：Using lysoplate assay, 900,000,000/ml bacterium solution is picked in thickness 5mm with aseptic cotton carrier
Agar plate on make uniformly intensive line, the punching of 6mm apertures card punch, fill it up with a kind of sample solution per hole, every kind of sample is molten
Liquid adds two holes to every kind of bacterium, and 37 DEG C of incubators act on 24h, measure antibacterial circle diameter, using completely not long bacterium as measurement standard, note
Record average value of every kind of sample to every kind of bacterium holes antibacterial circle diameter.
The antibacterial circle diameter of sample in 4 is can be seen that in more than 15mm from the result in table 4, wherein containing bitter oily chinaberry
The sample for setting oily nano-composition is eager to excel much than the fungistatic effect of the sample containing the free oily veepa oil of hardship, particularly embodiment
2nd, the inhibition zone of embodiment 3 is very notable up to 35~40mm, fungistatic effect.Illustrate the oily veepa oil nanometer group of hardship provided by the invention
Compound has good antibacterial, anti-inflammatory effect.
Bacteriostasis of the 4 different samples of table to 3 kinds of bacteriums
Embodiment 15
Comparative selection example 1, comparative example 2, embodiment 2 do externally applied anti-inflammation analgesic experimental study.
Take 28 ± 3.5g of body weight Kunming male mice 52, respectively 4 groups.First 80 μ L croton oils are drawn with micropipet to cause
Scorching liquid is applied to each mouse auris dextra tow sides, causes scorching half an hour after, accurately drawn according to group test specimen (comparative example 1, comparative example 2,
Embodiment 2, physiological saline) 50uL is applied to mouse right ear tow sides (being not added with rubbing), cause it is scorching after put to death mouse within 4 hours, cut
Left and right ear, the auricle for taking same area is beaten with 9mm card punch, is weighed.Using the auricle weight difference of left and right two as swelling degree, calculate
The swelling inhibiting rate of each sample, as a result sees table 5.
By the results showed that comparative example 1 of table 5, comparative example 2, embodiment 2 swelling inhibiting rate be respectively 28.9%,
54.1%th, 71.3%, the anti-inflammatory of the oily veepa oil nano-composition of hardship provided by the invention, analgesic effect are significantly better than free hardship
Oily veepa oil, illustrate that the oily veepa oil nano-composition of hardship provided by the invention significantly improves the anti-inflammatory of bitter oily veepa oil, analgesia
Effect.
The inhibitory action that the different sample external applications of table 5 are formed to mouse ear swelling
As seen from the above embodiment, the oily veepa oil nano-composition drugloading rate of hardship provided by the invention is big, good water solubility, steady
It is qualitative it is high, bioavailability is high, it is small to skin irritation, there is good mosquito repellent, antibacterial, anti-inflammatory, analgesia etc. to act on, and
Preparation method is simple, and chemical change does not occur, and does not change the chemical constitution of bitter oily veepa oil, and technique is easily controllable, suitable industry
Change, large-scale production, organic solvent is not used in whole preparation process, green, low energy consumption is safe.
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of bitter oily veepa oil nano-composition, include the component of following weight percentage：
Bitter oily veepa oil 1.0%~30.0%, emulsifying agent 25.0%~75.0%, assistant for emulsifying agent 0.8%~17.0%, polyalcohol
1.0%~65.0%, antioxidant 0.01%~2.0%, preservative 0.01%~4.0%.
2. the oily veepa oil nano-composition of hardship according to claim 1, it is characterised in that including following weight percentage
Component：
Bitter oily veepa oil 5.0%~25.0%, emulsifying agent 30.0%~65.0%, assistant for emulsifying agent 2.0%~15.0%, polyalcohol
5.0%~55.0%, antioxidant 0.05%~1.5%, preservative 0.05%~4.0%.
3. the oily veepa oil nano-composition of hardship according to claim 1 or 2, it is characterised in that the oily veepa oil of hardship is received
The particle diameter of rice composition is 20~150nm.
4. the oily veepa oil nano-composition of hardship according to claim 1, it is characterised in that the emulsifying agent includes poly- sorb
Ester emulsifying agent, Emulsifier EL-60 class emulsifying agent, cithrol class emulsifying agent and ceteareth class
One or more of mixtures in emulsifying agent.
5. the oily veepa oil nano-composition of hardship according to claim 1, it is characterised in that the assistant for emulsifying agent is to include two
Ethylene glycol monomethyl ether, butylol polyether -9, butylol polyether -12, butylol polyether -26, butylol polyether -35, PPG-26- butanol gather
One or more of mixtures in ether -26 and poloxamer.
6. the oily veepa oil nano-composition of hardship according to claim 1, it is characterised in that the polyalcohol includes poly- second two
Alcohol, propane diols, DPG, glycerine, 1,2- hexylene glycols, 1,3 butylene glycol, 1,2- pentanediols, octyl dodecanol, sorbierite and
One or more of mixtures in pungent glycol.
7. the oily veepa oil nano-composition of hardship according to claim 1, it is characterised in that the antioxidant includes dimension and given birth to
Plain C, VCE, vitamin E, Vitwas E, gallic acid, butylated hydroxy anisole, butylated hydroxy-a
One or more of mixtures in benzene, tert-butylhydroquinone, phytic acid, Rosmarinic acid and Tea Polyphenols.
8. the oily veepa oil nano-composition of hardship according to claim 1, it is characterised in that the preservative includes benzene oxygen second
One kind or several in alcohol, methylisothiazolinone, potassium sorbate, DMDMH, imidazolidinyl urea and Sensiva SC50
The mixture of kind.
9. the preparation method of bitter oily veepa oil nano-composition, comprises the following steps described in claim 1~8 any one：
Emulsifying agent, assistant for emulsifying agent, polyalcohol, antioxidant and preservative are mixed, obtain just batch mixing；
The just batch mixing is mixed with bitter oily veepa oil, obtains bitter oily veepa oil nano-composition.
10. preparation method described in bitter oily veepa oil nano-composition described in claim 1~8 any one or claim 9 obtains
To application of the bitter oily veepa oil nano-composition in cosmetics.
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Application publication date: 20171215 |