CN107466752A - A kind of screening technique of deep seeding tolerance Rice Germplasm Resources - Google Patents
A kind of screening technique of deep seeding tolerance Rice Germplasm Resources Download PDFInfo
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- CN107466752A CN107466752A CN201710644351.XA CN201710644351A CN107466752A CN 107466752 A CN107466752 A CN 107466752A CN 201710644351 A CN201710644351 A CN 201710644351A CN 107466752 A CN107466752 A CN 107466752A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
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Abstract
The invention provides a kind of screening technique of deep seeding tolerance Rice Germplasm Resources：Screened by collection material seed and broadcast preceding preparation sowing culture assay emergence rate and height of seedling index, be not less than 50% using emergence rate and height of seedling is not less than 5cm as standard, it is substandard to think its not deep seeding tolerance.It is subsoil that the present invention weighed the fine earths of 40 mesh sieves using 1 percent balances, and with the water of graduated cylinder measured amounts, improves the uniformity of experiment；The sieving fine earth for weighing different weight is blinding, represents different depths of planting respectively, and the accuracy and uniformity of experiment are improved compared with measuring blinding depth with ruler.
Description
Technical field
The invention belongs to agriculture new variety selective breeding technology field, and in particular to the screening side of deep seeding tolerance Rice Germplasm Resources
Method.
Background technology
As China's rural labor population shifts to city, the factor such as rural laborer's aging influences, Rice Production people
Expenses of labour increases year by year, and hired laborer is increasingly difficult to.Compared with traditional seedling method for transplanting, direct sowing of rice technology is a kind of letter
Just, saving of labor, laborsaving and efficient production technology, and substantial amounts of freshwater resources can be saved.Due to using direct seeding technique, rice
Seedling is not by caused damage of pulling up seedlings, and early growth condition is good, and nutrient growth is fast, can be than the rice precocity under transplanting condition
It is ripe.At present, solve the problems, such as direct sowing of rice crop smothering substantially using new herbicides, still, direct seading rice difficulty full stand and easily fall
The problem of volt is not solved effectively also.
Rice, which suitably seed deeplys, can effectively utilize soil moisture, increase the root system embedded depth of rice, reduce later stage lodging.
But depth of planting increase can increase emergence difficulty, cause out " disconnected ridge of being short of seedling ".Therefore, the screening of Rice Germplasm Resources deep seeding tolerance is carried out
Test Research, replace measuring blinding thickness with ruler by using 1 percent balance precise blinding weight, improve
The accuracy of screening technique, accurately to filter out, the native ability in top is strong, rapid deep seeding tolerance Rice Germplasm Resources of emerging provide safeguard.
The content of the invention
The problems such as experimental condition is inconsistent, test accuracy is low during the present invention tests for deep seeding tolerance, there is provided Yi Zhongnai
Seed deeply the screening technique of Rice Germplasm Resources, guarantee is provided to filter out deep seeding tolerance Rice Germplasm Resources.
To achieve the above object, the present invention adopts the following technical scheme that.
A kind of screening technique of deep seeding tolerance Rice Germplasm Resources, using following steps：
(1) collection material：Collect Rice Germplasm Resources；
(2) seed screens：100, full seed, unabroken seed is selected from each Rice Germplasm Resources；
(3) preceding preparation is broadcast：Seed is dipped to show money or valuables one carries unintentionally it is standby；Nutrition Soil crushes after drying；
(4) sow：With the sieving fine earth of 1 percent balance precise constant weights, the glass into high 17cm, diameter 6cm is contained
In cup, 150ml water is measured with graduated cylinder, gently pours into glass, underearth is impregnated with；Then will be selected in each resource material strong
Strengthen consistent 10, the seed that shows money or valuables one carries unintentionally and be uniformly placed in underearth surface；Afterwards constant weight is accurately weighed with 1 percent balances
Sieving fine earth is covered in the surface of the seed, represents seed level 6cm；Water spray moistens blinding completely but top layer is without ponding, uses plastic sheeting
Covering, keep moistening；
(5) cultivate：The glass sowed is positioned over phjytotron, 28 ± 2 DEG C of cultures；
(6) index determining：
Emergence rate：After sowing 3 days, the daily set time investigates emergence situation, calculates emergence rate, last time is adjusted within after planting 14 days
Look into emergence rate；
Height of seedling：After sowing 14 days, the length of investigation plumule section to seedling apical；
(7) interpretation of result：Test data calculates average value and the coefficient of variation, is not less than 50% with emergence rate and height of seedling is not less than
5cm is standard, substandard to think its not deep seeding tolerance.
In step (3), Nutrition Soil crosses 40 mesh sieves.
In step (4), subsoil weight is 300g, and blinding weight is 150g.
The present invention has following advantageous benefits：
It is subsoil that the present invention weighed the fine earths of 40 mesh sieves using 1 percent balances, and with the water of graduated cylinder measured amounts, is carried
The high uniformity of experiment；The sieving fine earth for weighing different weight is blinding, represents different depths of planting respectively, and with ruler
Measurement blinding depth compares the accuracy and uniformity for improving experiment.
Embodiment
With reference to embodiment, the present invention will be further described, but the present invention is not limited by following embodiments.
1. screening technique
(1) collection material：Collect holy rice 735, Haiyang upland rice, holy rice 22, holy rice 2572, holy rice 285, holy rice 20, holy perfume 145,
Holy military glutinous 0146, holy rice 15, holy rice 16, holy rice 18, fragrant rice 1601, it is purple fragrant glutinous 1306,9915, northern Shandong upland rice, totally 15 parts of resources
Material；
(2) seed screens：100, full seed, unabroken seed is selected from above-mentioned Rice Germplasm Resources；
(3) preceding preparation is broadcast：The seed filtered out immersion 24h is standby to showing money or valuables one carries unintentionally；Crushed after Nutrition Soil is dried, cross 40 mesh sieves；
(4) sow：The fine earth to be sieved with 1 percent balance precise 300g, contains the glass into high 17cm, diameter 6cm
In, 150ml water is measured with graduated cylinder, gently pours into glass, underearth is impregnated with；Select stalwartness in each material, show money or valuables one carries unintentionally unanimously
10, seed be uniformly placed in underearth surface；100g, 150g and 200g blinding are weighed respectively with 1 percent balances, respectively
Represent depth of planting 4cm, 6cm and 8cm；Blinding is moistened completely with watering can water spray but top layer is without ponding, with covered rearing with plastic film,
Keep moistening；Each material, which is set, to be repeated 3 times；
(5) cultivate：The glass sowed is positioned over phjytotron, in 60% time 28 ± 2 DEG C of temperature, humidity culture；
(6) index determining：Sowing starts after 3 days, set time every afternoon investigation emergence situation, stretches out surface with young shoot
More than 0.5cm is emergence, counts the emergence rate of each depth of planting of each material.After planting the 14th day last time statistics emergence rate；
(7) interpretation of result：Test data calculates average value and the coefficient of variation.It is not less than 50% with emergence rate and height of seedling is not less than
5cm is standard, substandard to think its not deep seeding tolerance.
2. result and analysis
The performance of the 2.1 different lower each morphological indexs of rice of seed level processing
Under the conditions of seed level 4cm, 6cm and 8cm, to the emergence rate of 15 parts of Rice Germplasm Resources of the holy grade of rice 735, height of seedling and reach most
The time of higher survival rate is identified.As a result such as table 1, with the increase of depth of planting, the emergence rate and height of seedling of each material are equal
It is on a declining curve, reach the time increase of highest emergence rate.But the degree of different materials change is different, if depth of planting is by 4cm
When increasing to 6cm, Haiyang upland rice, holy rice 2572, holy rice 20, holy perfume 145, the emergence rate of 5 parts of materials of northern Shandong upland rice are higher, super
90% is crossed, and emergence rate amplitude of variation is smaller, the range of decrease is within 5%；Holy rice 15, holy rice 18, holy rice 16, purple perfume glutinous 1306
Emergence rate changes greatly, and there is also difference between them, and one kind is such as holy rice 15 and holy rice 18, and they are in the processing of 4cm seed levels
Under emergence rate it is higher (be respectively 90% and 100%), and as depth of planting increases to 6cm, emergence rate drastically reduce (point
Wei 30% and 25%)；Another type is holy rice 16 and purple perfume glutinous 1306, and when 4cm seed levels are handled, emergence rate is not just high
(being respectively 70% and 55%), as depth of planting increases to 6cm, emergence rate is reduced to 30% and 0.Remaining 6 parts of material sowing
Depth increases to 6cm by 4cm, and the amplitude of variation of emergence rate is between 15%-30%.When depth of planting is 8cm, material goes out
Seedling rate is very low, and only holy rice 735, Haiyang upland rice, northern Shandong upland rice emergence rate are more than 15%, and most of material emergence rates are 0, not
The material of emergence does not also carry out the measure of height of seedling to its native inside points.
With the increase of depth of planting, height of seedling is in reduction trend, and depth of planting increases to 6cm by 4cm, and height of seedling reduces width
Only Haiyang upland rice of the degree less than 10%；The range of decrease has 5 parts (respectively between 20%-30%：Holy rice 22, holy rice 2572,
Holy rice 285, holy rice 20, northern Shandong upland rice)；The range of decrease has 5 parts (respectively more than 50%：Holy rice 15, holy rice 16, holy rice 18, purple
Fragrant glutinous 1306,9915)；The range of decrease of remaining 5 parts of material is between 30%-50%.
Under the conditions of different seed levels, the coefficient of variation of each Germplasms various trait is different, such as table 2.Sowed in 4cm
Under depth, the different materials emergence rate coefficient of variation is maximum, is secondly height of seedling, reaches the coefficient of variation of time of highest emergence rate most
It is small.Under 6cm depths of planting, the coefficient of variation of emergence rate and height of seedling is also larger, and reaches the variation of the time of highest emergence rate
Coefficient is smaller.Due to having more material not emerge under 8cm depths of planting, undetermined height of seedling can not also count it and reach and is most higher by
The time of seedling rate, therefore only count its emergence rate.
These results indicate that depth of planting has a great influence to the emergence rate and height of seedling of Rice Germplasm Resources material, can conduct
Screen the character observation index of deep seeding tolerance Rice Germplasm Resources.
Phenotypic evaluation of the 1 15 Rice Germplasm Resources materials of table under different depths of planting
2.2 each Rice Germplasm Resources deep seeding tolerance evaluations
Emergence rate is the leading indicator for evaluating Rice Germplasm Resources deep seeding tolerance, and emergence rate is higher, illustrates that deep seeding tolerance is stronger.This
Research is under the conditions of 8cm depths of planting, and only 8 parts of materials can emerge, but emergence rate is relatively low (≤25%), it is impossible to well
React the deep seeding tolerance ability of Rice Germplasm Resources.Under the conditions of 4cm depths of planting, emergence rate >=90%, height of seedling >=10cm, in 6cm
Under the conditions of depth of planting, emergence rate >=65% (average emergence rate), and height of seedling is more than 7.3cm (average height of seedling), shares 4 parts, point
It is not Haiyang upland rice, holy rice 2572, holy military glutinous 0146, northern Shandong upland rice, their deep seeding tolerance ability is stronger；4 parts of material deep seeding tolerances
Ability is weaker, is holy rice 15, holy rice 16, holy rice 18, purple perfume glutinous 1306 respectively, they do not emerge under the conditions of 6cm depths of planting
Or emergence rate is relatively low (being less than 50%)；Remaining 7 parts of material deep seeding tolerance ability is placed in the middle, its Zisco rice 285 and holy perfume 145 in 4cm and
Emergence rate reaches 90% under the conditions of 6cm depths of planting, but height of seedling is relatively low under the conditions of 6cm depths of planting, and this is probably its kind
Characteristic, its deep seeding tolerance ability are also stronger.
Influence of 2 different depths of planting of the table processing to the different Rice Germplasm Resources material coefficient of variation
Note：* represent that the characteristic index is unmeasured.
Claims (3)
1. a kind of screening technique of deep seeding tolerance Rice Germplasm Resources, it is characterised in that using following steps：
（1）Collection material：Collect Rice Germplasm Resources；
（2）Seed screens：100, full seed, unabroken seed is selected from each Rice Germplasm Resources；
（3）Broadcast preceding preparation：Seed is dipped to show money or valuables one carries unintentionally it is standby；Nutrition Soil crushes after drying；
（4）Sowing：The fine earth to be sieved with 1 percent balance precise constant weights, contains the glass into high 17cm, diameter 6cm
In cup, 150ml water is measured with graduated cylinder, gently pours into glass, underearth is impregnated with；Then will be selected in each resource material strong
Strengthen consistent 10, the seed that shows money or valuables one carries unintentionally and be uniformly placed in underearth surface；Afterwards constant weight is accurately weighed with 1 percent balances
Sieving fine earth is covered in the surface of the seed, represents seed level 6cm；Water spray moistens blinding completely but top layer is without ponding, uses plastic sheeting
Covering, keep moistening；
（5）Culture：The glass sowed is positioned over phjytotron, 28 ± 2 DEG C of cultures；
（6）Index determining：
Emergence rate：After sowing 3 days, the daily set time investigates emergence situation, calculates emergence rate, last time is adjusted within after planting 14 days
Look into emergence rate；
Height of seedling：After sowing 14 days, the length of investigation plumule section to seedling apical；
（7）Interpretation of result：Test data calculates average value and the coefficient of variation, is not less than 50% with emergence rate and height of seedling is not less than 5cm
It is substandard to think its not deep seeding tolerance for standard.
2. screening technique according to claim 1, it is characterised in that step（3）In, Nutrition Soil crosses 40 mesh sieves.
3. screening technique according to claim 1, it is characterised in that step（4）In, subsoil weight is 300g, blinding weight
Measure as 150g.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109601060A (en) * | 2018-11-21 | 2019-04-12 | 广东海洋大学 | A kind of screening plant of the resistance to deep germ plasm resource of rice convenient for comparison |
| CN113040012A (en) * | 2021-03-27 | 2021-06-29 | 河南省农业科学院粮食作物研究所 | Screening method of rice seeds directly sowed in dry mode after wheat growing |
| CN114532172A (en) * | 2022-02-22 | 2022-05-27 | 张家界爱德帮农业发展集团有限公司 | Reed rice series cultivation method and cultivation device |
| CN114747328A (en) * | 2022-04-08 | 2022-07-15 | 河南省农业科学院粮食作物研究所 | Screening device capable of detecting deep-sowing-resistant condition of rice germplasm |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109601060A (en) * | 2018-11-21 | 2019-04-12 | 广东海洋大学 | A kind of screening plant of the resistance to deep germ plasm resource of rice convenient for comparison |
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| CN113040012A (en) * | 2021-03-27 | 2021-06-29 | 河南省农业科学院粮食作物研究所 | Screening method of rice seeds directly sowed in dry mode after wheat growing |
| CN114532172A (en) * | 2022-02-22 | 2022-05-27 | 张家界爱德帮农业发展集团有限公司 | Reed rice series cultivation method and cultivation device |
| CN114747328A (en) * | 2022-04-08 | 2022-07-15 | 河南省农业科学院粮食作物研究所 | Screening device capable of detecting deep-sowing-resistant condition of rice germplasm |
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Application publication date: 20171215 |