CN106508459A - Crop whole growth period salt tolerance screening and identifying method - Google Patents
Crop whole growth period salt tolerance screening and identifying method Download PDFInfo
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- CN106508459A CN106508459A CN201611116559.6A CN201611116559A CN106508459A CN 106508459 A CN106508459 A CN 106508459A CN 201611116559 A CN201611116559 A CN 201611116559A CN 106508459 A CN106508459 A CN 106508459A
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Abstract
The invention discloses a crop whole growth period salt tolerance screening and identifying method. The method includes the specific implementation steps that A, field plough layer soil is taken, and the salt content of the soil is measured; B, a corresponding amount of NaCl is added into the soil, and salinized soil with the corresponding salt concentration is prepared; C, a water seepage siphoning automatic irrigation device is adopted for autonomously adjusting the water supply speed to the soil according to the humidity of the soil, and the steady state of soil salt stress intensity of the soil is maintained; D, under the same or different salt concentrations, the crop whole growth period is subjected to salt stress treatment, and the crop variety salt tolerance is comprehensively screened and identified according to the survival rate, the single plant seed grain number, the single plant seed yield, protein, the oil content and other characters. A test place is subjected to rain blocking through a transparent material, the influence from the environment is low, conditions are controllable, cost is low, accuracy and high efficiency are achieved, and the identification result is precise; a basis is provided for breeding of salt tolerance crop varieties, and a scientific standard is provided for evaluation of crop salt tolerance grades.
Description
Technical field
The present invention relates to field of plant variety breeding technology, more particularly to a kind of crop time of infertility selection of salt tolerance and identification side
Method.
Background technology
The soil salinization can suppress the reproductive growth of crop plant and material storage, make crop leaf chlorosis even withered,
Cause crop yield and the decline of quality.By global warming, industrial pollution aggravation and chemical fertilizer application be improper etc., factor is affected,
The soil salinization is on the rise.For this purpose, in addition to improved soil, convenient, stable, accurately salt tolerance of crop Screening and Identification is set up
System, selection-breeding and plantation salt tolerant crop, the yield for increasing crop under salt stress are always focus of concern.
As land for growing field crops authentication method is by climatic factor and the unequal such environmental effects of plot saliferous such as temperature, rainfalls
It is larger, and need the salt tolerance of material could be reliably identified and be evaluated by result of the test for many years, test week
Phase length, high cost.Although the authentication method experiment condition such as potted plant, salt pond is controllable, and multiple kinds can be compared simultaneously
Compared with, but due to the soil body it is less, according to existing test method of supplying water, because between plant, growth differences can affect soil moisture content and salt
Coercive intensity, and soil moisture content and salt stress intensity are the important envirment factor of plant growth, therefore, have impact on it is potted plant,
The reliability of the crop time of infertility selection of salt tolerance authentication method such as salt pond.
For this purpose, in the urgent need to find to crop time of infertility salt tolerance efficiently, accurately process for screening and identifying.The present invention is carried
A feasible solution is supplied, the selection-breeding for salt tolerant crop kind provides foundation, and provides for crop tolerance to salt level evaluation
The standard of science.
The content of the invention
Deficiency of the present invention for existing salt tolerance of crop process for screening and identifying, proposes a kind of crop time of infertility salt tolerance
Screening and authentication method, experimental result less, condition affected by environment are controllable and with low cost, precise and high efficiency, qualification result
Accurately.Can be using the method for present invention offer come clearly each crop varieties and the tolerable salt stress intensity of germ plasm resource；For making
Thing germ plasm resource Large-scale Screening, the suitable salinity of selection-breeding of salt tolerant crop kind and different salt tolerant ranks provide certain section
Learn standard and judgment.
In order to realize foregoing invention purpose, the technical problem of the present invention is mainly addressed by following technical proposals：
A kind of crop time of infertility selection of salt tolerance and authentication method, implementing step includes：
A, field topsoil is taken, measure and calculation total soil phosphorus；
B, the total soil phosphorus calculated according to step A tester, add respective amount NaCl in above-mentioned soil, and use bottom
The mode of portion's water suction is filled thoroughly, makes the salinized soil of corresponding salinity；
C, according to soil dry humidity, using feed rate of the automatic watering device from main regulation to soil, make Soil conservation
Suitable humidity, maintains the stable state of soil salt stress intensity；
D, under same or different salinity, salt stress process is carried out to the time of infertility, according to plant growth character pair make
Thing Variety utilization is screened comprehensively and is identified.
Further, adopt saturating at the top of a kind of crop time of infertility selection of salt tolerance and the test site of authentication method
Bright material carries out process of keeping off the rain.
Further, in step A, total soil phosphorus measure and calculation method is：
By field soil drying, mixing, remove impurity, the pack of the soil sample after 1mm sieve apertures was taken, soil sampling 18.00g is put into
90mL is without CO2In the beaker of distilled water, 3min is accurately vibrated on vibrating machine, 25 DEG C of calorstats is put into, is filtered soil extract
20mL is put in the porcelain evaporating dishes of dries quantity known to 100mL, is evaporated on water-bath, uses repeatedly H2O2Residue is processed, is made organic
Matter complete oxidation residue is presented white, and the residue and evaporating dish after being evaporated dries 1～2 hour to constant weight in being placed on 105 DEG C of baking ovens,
Weigh after cooling, according to formula
Calculate and be unearthed
Earth total salt content.
Further, the method for the salinized soil for preparing corresponding salinity in step B is：
The plastic tub that hole is arranged at bottom is placed on pallet, the field topsoil for being dried, sieving is put in basin, according to step
The total soil phosphorus that rapid A testers are calculated, substitute into formula
NaCl additions=(preparing the specific salinity-total soil phosphorus prepared) × 8000, calculate NaCl additions
Amount, adds corresponding NaCl, makes the salinized soil of corresponding salinity.
Further, when the siphon automatic watering device that seeps water in step C is ceramic head infiltration siphon automatic flow watering device,
Which uses step as follows：
Ceramic head infiltration siphon automatic flow watering device ceramic segment is put in water and is soaked more than 10 minutes, by ready bottle
The narrower tank of mouth fills water, and soaked ceramic head is filled water, will be ceramic head infiltration siphon automatic flow watering device another
One end is put into the narrower tank of bottleneck, after determining that ceramic head fills water, vinyl cover is covered tightly with ceramic head, and ceramic head is whole
Body inserts soil, and on position is throughout managed sample room and is consistent, when the soil body is larger, can be with multiple ceramic heads infiltration siphon certainly
Dynamic flow watering device.
Ceramic head infiltration siphon automatic flow watering device can independently adjust feed rate to soil according to soil dry humidity, make soil
Earth keeps suitable humidity.
Further, biological growth character crop varieties salt tolerance screened comprehensively in step D and identified
Including survival rate, single-strain seed grain number, seed yield per plant, protein and oil content content.
For example, Semen sojae atricolor can be made using survival rate, single-strain seed grain number, seed yield per plant and protein content etc.
For identification of indicator；Can be using single plant yield, seed oil content, unit area oil production etc. as identification of indicator to Brassica campestris L.
Beneficial effects of the present invention：
1st, the inventive method test site carries out process of keeping off the rain using transparent material, and experimental result is affected by environment less,
Condition is controllable, with low cost, and precise and high efficiency, qualification result are accurate.
2nd, can be using the method for present invention offer come clearly each crop varieties and the tolerable salt stress intensity of germ plasm resource；
It is that the suitable salinity of the selection-breeding of crop germplasm resource Large-scale Screening, salt tolerant crop kind and different salt tolerant ranks are provided necessarily
Scientific and standard and foundation.
3rd, the inventive method is simple to operate, and repeatability is good.
4th, the inventive method gears to actual circumstances, and is widely used in plant germplasm Screening of Germplasm and evaluation, and what is filtered out is resistance to
Salt resource has preferable production application prospect and promotion potential.
Specific embodiment
Below the preferred embodiments of the present invention are described in detail so that advantages and features of the invention can be easier to by
It will be appreciated by those skilled in the art that apparent clearly defining so as to make to protection scope of the present invention.
Embodiment 1
Application of the inventive method in the identification of Semen sojae atricolor time of infertility selection of salt tolerance.
From Williams 82, day is grand No. 1, the magnificent spring No. 3, tetra- Semen sojae atricolor strains of J1, wherein Williams82 is salt density value
Material, grand No. 1 of day is moderate salt-enduring cultivars, and the magnificent spring No. 3 is salt-enduring cultivars, and J1 is salt tolerant transgenic line.In 2 materials of every basin kind
Material, each kind random pair but does not repeat；Each material is each to process 8 seeds of sowing, and field planting after emerging is 4 plants, and test sets 3 times
Repeat.
A kind of soybean crops time of infertility selection of salt tolerance and authentication method, which implements step includes：
A, field topsoil is taken, drying, mixing, remove impurity took the pack of the soil sample after 1mm sieve apertures, soil sampling
18.00g, is put into 90mL without CO2In the beaker of distilled water, 3min is accurately vibrated on vibrating machine, 25 DEG C of calorstats is put into, is filtered
Soil extract 20mL is put in the porcelain evaporating dishes of dries quantity known to 100mL, is evaporated on water-bath, uses repeatedly H2O2Process
Residue, makes organic matter complete oxidation residue that white is presented, and the residue and evaporating dish after being evaporated dries 1～2 in being placed on 105 DEG C of baking ovens
Hour is weighed after cooling, according to formula to constant weight
Calculate
Go out total soil phosphorus for 0.15%.
B, bottom there is is the plastic tub in hole be placed on pallet, in basin, is put into the field topsoil that 8kg is dried, sieves,
According to the total soil phosphorus 0.15% that step A tester is calculated, formula is substituted into
NaCl additions=(preparing the specific salinity-total soil phosphorus prepared) × 8000, calculate NaCl additions
Amount, adds corresponding NaCl, makes the salinized soil of 0.6% salinity.
C, siphon automatic flow watering device ceramic segment that ceramic head seeps water are put in water and soak more than 10 minutes, will be ready
The narrower tank of bottleneck fills water, and soaked ceramic head is filled water, by ceramic head infiltration siphon automatic flow watering device
The other end is put into the narrower tank of bottleneck, after determining that ceramic head fills water, vinyl cover is covered tightly with ceramic head, ceramic head
Overall insertion soil, on position is throughout managed sample room and is consistent, when the soil body is larger, can be with the infiltration siphon of multiple ceramic heads
Automatic flow watering device.
Ceramic head infiltration siphon automatic flow watering device can independently adjust feed rate to soil according to soil dry humidity, make soil
Earth keeps suitable humidity.
D, periodic statistical difference Semen sojae atricolor strain n plant survival rate, investigate single-strain seed grain number, seed yield per plant after results.
It is as shown in table 1 for trying soybean varieties (being) yield and economical character under the process of 0.6% soil total salt amount salt stress.From table 1
As a result as can be seen that under 0.6% soil total salt amount salt stress, although each to have certain emerging for examination soybean varieties (being)
Rate, but in vegetative growth phase, Williams 82 is not resistant to the salt stress of the intensity, and plant is withered, no yield, its
It is remaining to have certain toleration for examination soybean varieties (being) to the salt stress of the intensity.It is from the point of view of the final seed production for harvesting, resistance to
Salt is followed successively by Williams 82<It grand No. 1<J1<The magnificent spring No. 3.
Table 1 yield and economical character under the process of 0.6% soil total salt amount salt stress for examination soybean varieties (being)
Process of keeping off the rain is carried out using transparent material at the top of its test site.
As a result show：This method can provide foundation for the screening of Soybean and Other Crops Salt-tolerant Materials, and clear and definite Soybean and Other Crops can
With the Soil salinity for tolerating.
Embodiment 2
Application of the inventive method in the identification of Brassica campestris L selection of salt tolerance.
From Zhejiang pair 72, tetra- Brassica campestris L strains of SEM3, DSEM3 and SEM6, wherein Zhejiang double 72 is pushed away for In Middle And Lower Reaches of Changjiang River
Wide kind, is Brassica campestris L Salt tolerance check variety, and SEM3, DSEM3, SEM6 are Jing Salt Tolerance at Seedling Stage identifications in many years, it was demonstrated that salt tolerance
It is substantially better than the napus lines in Zhejiang double 72.In 2 materials of every basin kind, each kind random pair but do not repeat；Using every basin kind 1
Strain, test set 10 repetitions.
A kind of cole crop time of infertility selection of salt tolerance authentication method and system, which implements step includes：
A, field topsoil is taken, drying, mixing, remove impurity took the pack of the soil sample after 1mm sieve apertures, soil sampling
18.00g, is put into 90mL without CO2In the beaker of distilled water, 3min is accurately vibrated on vibrating machine, 25 DEG C of calorstats is put into, is filtered
Soil extract 20mL is put in the porcelain evaporating dishes of dries quantity known to 100mL, is evaporated on water-bath, uses repeatedly H2O2Process
Residue, makes organic matter complete oxidation residue that white is presented, and the residue and evaporating dish after being evaporated dries 1～2 in being placed on 105 DEG C of baking ovens
Hour is weighed after cooling, according to formula to constant weight
Calculate
Go out total soil phosphorus for 0.15%.
B, bottom there is is the plastic tub in hole be placed on pallet, in basin, is put into the field topsoil that 8kg is dried, sieves,
According to the total soil phosphorus 0.15% for calculating, formula is substituted into
NaCl additions=(preparing the specific salinity-total soil phosphorus prepared) × 8000, calculate NaCl additions
Amount, adds corresponding NaCl, makes the salinized soil of 0.45% salinity.
C, siphon automatic flow watering device ceramic segment that ceramic head seeps water are put in water and soak more than 10 minutes, will be ready
The narrower tank of bottleneck fills water, and soaked ceramic head is filled water, by ceramic head infiltration siphon automatic flow watering device
The other end is put into the narrower tank of bottleneck, after determining that ceramic head fills water, vinyl cover is covered tightly with ceramic head, ceramic head
Overall insertion soil, on position is throughout managed sample room and is consistent, when the soil body is larger, can be with the infiltration siphon of multiple ceramic heads
Automatic flow watering device.
Ceramic head infiltration siphon automatic flow watering device can independently adjust feed rate to soil according to soil dry humidity, make soil
Earth keeps suitable humidity.
The different Brassica campestris L strain single plant yields of D, statistics, seed oil content, per mu yield oil mass.Table 2 is shown under condition of salt stress
Salt tolerant Brassica campestris L and the oil content and yield forming characteristic of control, the result of table 2 are can be seen that in 0.45% soil total salt condition
Under, the single plant yield of 3 salt-resistance strains SEM3, DSEM3, SEM6, seed oil content are all remarkably higher than check variety Zhejiang double 72, point
Gao Yu not check variety 122.51%, 154.93% and 182.00%.
The oil content and yield forming characteristic of salt tolerant Brassica campestris L and control under 2 potted plant condition of salt stress of table
Table 3 supplies examination rape variety (being) yield and quality measurement result in the case of showing saline land actual plantation, using this
The salt tolerant Brassica campestris L material yield and oil content that patent methods described Screening and Identification goes out still is significantly higher than check variety Zhejiang double 72, its
As a result it is consistent with results from pot experiment test general trend, illustrate that this patent methods described can be applied to the salt tolerant of the crops such as Brassica campestris L
Property Screening and Identification.
Table 3 is for yield, oil content performance under examination rape variety (being) saline land planting conditions
Process of keeping off the rain is carried out using transparent material at the top of its test site.
As a result show, this method can provide foundation for the screening of the crop tolerance to salt such as Brassica campestris L material, and the crop such as clear and definite Brassica campestris L can
With the Soil salinity for tolerating.
Emergence rate：The seed number of emergence rate=number of emerging ÷ sowing.
Survival rate：After planting the regular period counts each material and survives strain number, and n plant survival rate (%)=(survival plant number/
Field planting strain number) × 100.
Seed yield per plant：Individual plant harvest crop seed dry after quality.
Single-strain seed grain number：After plant maturation, determine each for studying seed grain number of the thing material per individual plant.
Oil content：Can be measured using methods such as soxhlet extraction methods, near infrared spectroscopy, nuclear magnetic resonance methods.
Per mu yield oil mass：Can directly measure or be calculated according to seed oil content, planting density, individual plant or cell production and obtain.
Beneficial effects of the present invention：
1st, the inventive method test site carries out process of keeping off the rain using transparent material, and experimental result is affected by environment less,
Condition is controllable, with low cost, and precise and high efficiency, qualification result are accurate.
2nd, can be using the method for present invention offer come clearly each crop varieties and the tolerable salt stress intensity of germ plasm resource；
For crop germplasm resource Large-scale Screening, and the suitable salinity of selection-breeding and different salt tolerant ranks of salt tolerant crop kind are provided
Certain scientific and standard and foundation.
3rd, the inventive method is simple to operate, and repeatability is good.
4th, the inventive method gears to actual circumstances, and is widely used in plant germplasm resource screening and evaluates, the salt tolerant money for filtering out
Source has preferable production application prospect and promotion potential.
The above, the only specific embodiment of the present invention, but protection scope of the present invention is not limited thereto, any
The change or replacement expected without creative work, should all be included within the scope of the present invention.Therefore, it is of the invention
The protection domain that protection domain should be limited by claims is defined.
Claims (6)
1. a kind of crop time of infertility selection of salt tolerance and authentication method, it is characterised in that implementing step includes：
A, field topsoil is taken, measure and calculation total soil phosphorus；
B, the total soil phosphorus calculated according to step A tester, add respective amount NaCl in above-mentioned soil, and are inhaled with bottom
The mode of water is filled thoroughly, makes the salinized soil of corresponding salinity；
C, according to soil dry humidity, using feed rate of the automatic watering device from main regulation to soil, make Soil conservation suitable
Humidity, maintain soil salt stress intensity stable state；
D, under same or different salinity, salt stress process is carried out to the crop time of infertility, according to plant growth character pair make
Thing Variety utilization is screened comprehensively and is identified.
2. a kind of crop time of infertility selection of salt tolerance and authentication method according to claim 1, it is characterised in that it is described its
Rain-repellent canopy is provided with the top of the test site of method.
3. a kind of crop time of infertility selection of salt tolerance and authentication method according to claim 1, it is characterised in that the step
In rapid A, total soil phosphorus measure and calculation method is：
By field soil drying, mixing, remove impurity, took the soil sample after 1mm sieve apertures pack, soil sampling 18.00g, be put into 90mL without
CO2In the beaker of distilled water, 3min is accurately vibrated on vibrating machine, 25 DEG C of calorstats are put into, soil extract 20mL is filtered and is put
Enter in the porcelain evaporating dishes of dries quantity known to 100mL, be evaporated on water-bath, use repeatedly H2O2Residue is processed, makes organic matter complete
Oxidation residua is presented white, and the residue and evaporating dish after being evaporated dries 1～2 hour to constant weight in being placed on 105 DEG C of baking ovens, after cooling
Weigh, according to formula：
Calculate soil
Total salt content.
4. a kind of crop time of infertility selection of salt tolerance and authentication method according to claim 1, it is characterised in that the step
The method of the salinized soil for preparing corresponding salinity in rapid B is：
The plastic tub that hole is arranged at bottom is placed on pallet, the field arable layer for being dried, sieving in basin, is put into
Soil, according to the total soil phosphorus that step A tester is calculated, substitutes into formula
NaCl additions=(preparing the specific salinity-total soil phosphorus prepared) × 8000,
NaCl additions are calculated, corresponding NaCl is added, is made the salinized soil of corresponding salinity.
5. a kind of crop time of infertility selection of salt tolerance and authentication method according to claim 1, it is characterised in that the step
When in rapid C, automatic watering device is ceramic head infiltration siphon automatic flow watering device, which uses step as follows：
By ceramic head infiltration siphon automatic flow watering device ceramic segment be put in water soak more than 10 minutes, by ready bottleneck compared with
Narrow tank fills water, and soaked ceramic head is filled water, by the ceramic head infiltration siphon automatic flow watering device other end
The narrower tank of bottleneck is put into, after determining that ceramic head fills water, vinyl cover is covered tightly with ceramic head, ceramic head is integrally inserted
Enter soil, on position is throughout managed sample room and is consistent, when the soil body is larger, with multiple ceramic heads infiltration siphon automatic flower watering
Device.
6. a kind of crop time of infertility selection of salt tolerance and authentication method according to claim 1, it is characterised in that the step
Biological growth character crop varieties salt tolerance screened comprehensively in rapid D and identified include survival rate, single-strain seed grain number,
Seed yield per plant, protein content and oil content.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107941801A (en) * | 2017-12-29 | 2018-04-20 | 青岛袁策生物科技有限公司 | A kind of method of high frequency zone salt alkaline tolerance rice |
| CN108169470A (en) * | 2017-12-29 | 2018-06-15 | 广州玛夫信息科技有限公司 | A kind of saline-alkali tolerant laboratory |
| CN108739196A (en) * | 2018-05-18 | 2018-11-06 | 江苏省农业科学院 | A kind of soybean salt-tolerance composite sifting and identification method |
| CN108834796A (en) * | 2018-07-13 | 2018-11-20 | 江苏沿海地区农业科学研究所 | A kind of construction method of rice at whole growth periods Salt-Tolerance Identification culture apparatus and application |
| CN109362547A (en) * | 2018-11-15 | 2019-02-22 | 江苏沿江地区农业科学研究所 | A kind of identification method of semen viciae fabae salt tolerance |
| CN114051855A (en) * | 2021-11-24 | 2022-02-18 | 安徽农业大学 | Field test method for researching stress resistance of different wheat lines in same growth period |
| CN117235322A (en) * | 2023-11-14 | 2023-12-15 | 中国农业大学 | Crop salt-tolerant high-product seed screening method and device, electronic equipment and storage medium |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107941801A (en) * | 2017-12-29 | 2018-04-20 | 青岛袁策生物科技有限公司 | A kind of method of high frequency zone salt alkaline tolerance rice |
| CN108169470A (en) * | 2017-12-29 | 2018-06-15 | 广州玛夫信息科技有限公司 | A kind of saline-alkali tolerant laboratory |
| CN108739196A (en) * | 2018-05-18 | 2018-11-06 | 江苏省农业科学院 | A kind of soybean salt-tolerance composite sifting and identification method |
| CN108739196B (en) * | 2018-05-18 | 2020-09-04 | 江苏省农业科学院 | Salt tolerance compound screening and identifying method for soybeans |
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| CN114051855A (en) * | 2021-11-24 | 2022-02-18 | 安徽农业大学 | Field test method for researching stress resistance of different wheat lines in same growth period |
| CN117235322A (en) * | 2023-11-14 | 2023-12-15 | 中国农业大学 | Crop salt-tolerant high-product seed screening method and device, electronic equipment and storage medium |
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