CN107460218A - The method that a kind of low alkali of microwave radiation technology produces mannan-oligosaccharides from palm kernel meal - Google Patents
The method that a kind of low alkali of microwave radiation technology produces mannan-oligosaccharides from palm kernel meal Download PDFInfo
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- CN107460218A CN107460218A CN201710726878.7A CN201710726878A CN107460218A CN 107460218 A CN107460218 A CN 107460218A CN 201710726878 A CN201710726878 A CN 201710726878A CN 107460218 A CN107460218 A CN 107460218A
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- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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Abstract
The invention discloses the method that a kind of low alkali of microwave radiation technology produces mannan-oligosaccharides from palm kernel meal, it is characterised in that by the palm kernel meal microwave radiation exaraction after crushing；After mixed liquor press filtration or centrifugation, supernatant is taken, its pH value is adjusted to 7, is concentrated in vacuo after digesting 7 12 hours using mannase；Concentrate is centrifuged after alcohol precipitation, obtained sediment is freeze-dried, that is, obtains mannan-oligosaccharides crude product.The present invention is using palm kernel meal as raw material; handled after pulverizing and sieving using microwave radiation technology alkali; reduce the dosage of alkali and improve the extraction effect of alkali; after separation of solid and liquid; obtain the palm kernel meal prefinished products with good hydrolysis result; reduce the inhibition that lignin is degraded to palm kernel meal mannosan; so as to effectively improve the efficiency that palm kernel meal mannosan is converted into mannan-oligosaccharides; and reduce chemical levels; be advantageous to environmental protection; production cost is reduced, so as to produce higher economic value.
Description
Technical field
The present invention relates to the method that a kind of low alkali of microwave radiation technology produces mannan-oligosaccharides from palm kernel meal, belong to feature
Oligosaccharides technical field.
Background technology
Palm kernel meal is the byproduct after palm kernel shelling oil expression, and mannan content is very high in palm kernel meal, is to prepare
The optimum feed stock of mannan-oligosaccharides.Research finds that 78% is that straight chain mannan (contains seldom in palm kernel meal SNSP
The galactolipin of amount), 12% cellulose, 3% glucuronic acid xylan and 3% araboxylan (Dusterhoft
Deng 1992).Daud and Jarvis (1992) are also reported, a small amount of galactomannans, palm kernel meal are also contained in palm kernel meal
In galactomannans be probably soluble non-fibrillar element polysaccharide sub-fraction.Mannosan in palm kernel meal is that have
The polymer of the mannose of β side chains, it account for total solids 39% (Daud and Jarvis, 1992；Dusterhoft etc., 1992；
Knudsen, 1997).Most of mannosan is all very firm in palm kernel meal, highly crystalline and not soluble in water
(Aspinal, 1970), however, Dusterhoft etc. (1992) has found that the mannosan in about 66% palm kernel meal can be
Dissolved after a series of processing of alkali and sodium chloride.
At present, to the comprehensive utilization technique of palm kernel meal, it is concentrated mainly on after fermentation as feed, is raised for nonruminant
Hello, with improve palm kernel meal use value (it is identical, 2012；Soltan, 2009；A.Kumar etc., 2008；DAIRO etc.,
2007；NgWingKeong, 2004).And the document that mannan-oligosaccharides product is prepared with palm kernel meal is less, Wang Shaoyun etc.
(2009) report using palm kernel meal as substrate, degrade to obtain manna oligosacchride crude product using neutral β-Mannannase, and to it
Wherein each component is isolated and purified using paper chromatography, finally gives three kinds of mannobiose, manninotriose and mannotetrose oligosaccharides
Component.But our research indicate that, with mannosan ferment treatment palm kernel meal, its mannan-oligosaccharides recovery rate is less than 1%, no
Foot carries out industrialized production mannan-oligosaccharides in this way.In addition, we also trial with soda acid handle palm kernel meal, industrialized
Mannan-oligosaccharides are extracted, its recovery rate is stable between 8-12%, but accessory substance is more, subsequent treatment heavy load, in view of ring
Packing pressure, large-scale production are remained in problem.
At present, mannan-oligosaccharides are produced from palm kernel meal, there are three kinds of approach, first, passing through acid and alkali hydrolysis method；Second, pass through
Enzymatic isolation method；Third, pass through fermentation method.The defects of dosage is big, temperature is high, environmental protection treatment requirement is high be present in acid and alkali hydrolysis method.Enzymatic isolation method
Advantages, as temperature is low, advantages of environment protection, but due in palm kernel meal most of mannosan it is all very firm, it is high
Degree crystallization and also it is not soluble in water, its extraction is very low, does not possess Industrialized conditions.Fermentation method is although simple and easy to get, but product is answered
Miscellaneous, there is problem in separation and Extraction, be unfavorable for obtaining high-purity mannan oligosaccharide.
Microwave is as a kind of modernization biomass processing technology, and the efficiency of heating surface is high, treatment temperature is low and homogeneous heating.Microwave
Report of the technology in terms of lignocellulosic is handled is more, a kind of lignocellulosic pretreatment of microwave radiation technology alkaline sodium sulfite
Method (the patent No.：CN201510251408.0), microwave radiation can to chitosan prepare and performance influence (Yan Baifen,
1997), the research (Hu Siqian, 2003) of Preparation of Chitosan by Microwave, the microwave radiation technology liquefaction degraded of Wheat Straw Lignin from Alkali Pulping
(Ou Yangxinping, 2014) etc..At present, enzymic degradation palm kernel meal extraction mannan-oligosaccharides report, its mannan-oligosaccharides are only seen
For recovery rate less than 1%, cost is higher, and industrialization is distant.
The content of the invention
Problem to be solved by this invention is to provide a kind of side for producing mannan-oligosaccharides from palm kernel meal using microwave
Method.
In order to solve the above problems, it is oligomeric that sweet dew is produced from palm kernel meal the invention provides a kind of low alkali of microwave radiation technology
The method of sugar, it is characterised in that comprise the following steps：
Step 1)：Palm kernel meal pre-treatment：Palm kernel meal is crushed into fineness to sieve for 50-400 mesh；
Palm kernel meal is very firm, its contained mannosan highly crystalline, and not soluble in water, so first having to palm
The benevolence dregs of rice carry out ultramicro grinding.
Step 2)：By the palm kernel meal after crushing add water stir after microwave radiation exaraction, pH value is adjusted using alkali lye
To 8-13,6-14 is more than minute for processing；
Step 3)：After mixed liquor press filtration or centrifugation that step 2) is obtained, supernatant is taken, its pH value is adjusted to 7, use is sweet
Dew is concentrated in vacuo after dextranase enzymolysis 7-12 hours；
Step 4)：The concentrate that step 3) is obtained is centrifuged after alcohol precipitation, and obtained sediment is freeze-dried, and is produced
To mannan-oligosaccharides crude product.
Preferably, the frequency of microwave is 2450mHz ± 50Hz in the step 2).
Preferably, the weight of palm kernel meal and water ratio is 1: 5 in the step 2).
Preferably, pH value is adjusted to 10 during microwave radiation exaraction in the step 2), handles 10-14 minutes.
Preferably, for the alcohol that alcohol precipitation uses in the step 4) for the ethanol of mass concentration 95%, its volumetric usage is concentration
3 times of liquid.
The present invention is handled using microwave radiation technology alkali after pulverizing and sieving using palm kernel meal as raw material, reduces the use of alkali
Measure and improve the extraction effect of alkali, after separation of solid and liquid, obtain the palm kernel meal prefinished products with good hydrolysis result, reduce
The inhibition that lignin is degraded to palm kernel meal mannosan, sweet dew is converted into so as to effectively improve palm kernel meal mannosan
The efficiency of oligosaccharide, and chemical levels are reduced, be advantageous to environmental protection, production cost reduced, so as to produce higher warp
Ji value.
Brief description of the drawings
Fig. 1 be embodiment 1 in palm kernel meal crush 90 now extract HPLC figure；
Fig. 2 is the HPLC figures of extract under palm kernel meal pH=9 in embodiment 2；
Fig. 3 is the HPLC figures of extract under palm kernel meal microwave radiation exaraction 10min in embodiment 3；
In Fig. 1-3：Label 1 is mannose peak；
Fig. 4 is the tables of data of palm kernel meal mannan-oligosaccharides yield under different pH value in embodiment 2；
Fig. 5 is the tables of data of palm kernel meal mannan-oligosaccharides yield under different microwave irradiating times in embodiment 3.
Embodiment
To become apparent the present invention, hereby with preferred embodiment, and accompanying drawing is coordinated to be described in detail below.
The process equipment of microwave is back yard industry micro-wave oven in embodiment 1-3, and its preset parameter is：Input power (three-phase
Five line 380V ± 10%50Hz ± 1%), rated input power (≤5kVA), microwave output power (>=3kW, adjustable), microwave
Frequency (2450MHz ± 50Hz).
Embodiment 1
The method that a kind of low alkali of microwave radiation technology produces mannan-oligosaccharides from palm kernel meal：
Step 1)：Palm kernel meal pre-treatment：It is respectively 50-60,80-100,200,400 that palm kernel meal is crushed into fineness
Mesh sieves；
Step 2)：Palm kernel meal after crushing is pressed into palm kernel meal：Water=1: after 5 add water to stir, Microwave-assisted Extraction
Take, the frequency of microwave is 2450mHz, and pH value is adjusted into 10 using alkali lye, handled more than 10 minutes；
Step 3)：After mixed liquor press filtration or centrifugation that step 2) is obtained, supernatant is taken, its pH value is adjusted to 7, use is sweet
Dew is concentrated in vacuo after dextranase enzymolysis 7-12 hours；
Step 4)：The concentrate that step 3) is obtained is centrifuged after alcohol precipitation, and obtained sediment is freeze-dried, and is produced
To mannan-oligosaccharides crude product, for the alcohol that alcohol precipitation uses for the ethanol of mass concentration 95%, its volume is 3 times of concentrate.
Influence of the palm kernel meal smashing fineness of table 1 to mannan-oligosaccharides recovery rate
| Crush mesh number | 50-60 | 80-100 | 200 | 400 |
| Recovery rate (%) | 4.9 | 10.5 | 14.1 | 16.7 |
Table 1 is visible, with mesh number increase is crushed, to using mannan-oligosaccharides in microwave radiation technology low-alkali treatment palm kernel meal
Recovery rate, be continuously increased, but more than 200 mesh after, recovery rate increase is slow.
Embodiment 2
The method that a kind of low alkali of microwave radiation technology produces mannan-oligosaccharides from palm kernel meal：
Step 1)：Palm kernel meal pre-treatment：Palm kernel meal is crushed into fineness to sieve for 200 mesh；
Step 2)：Palm kernel meal after crushing is pressed into palm kernel meal：Water=1：After 5 add water to stir, Microwave-assisted Extraction
Take, the frequency of microwave is 2450mHz, pH value is adjusted to 8,9,10,11,12 respectively using saturation sodium hydroxide solution, processing 10
More than minute；
Step 3)：After mixed liquor press filtration or centrifugation that step 2) is obtained, supernatant is taken, its pH value is adjusted to 7, use is sweet
Dew dextranase is concentrated in vacuo after digesting 10 hours；
Step 4)：The concentrate that step 3) is obtained is centrifuged after alcohol precipitation, and obtained sediment is freeze-dried, and is produced
To mannan-oligosaccharides crude product, the alcohol that middle alcohol precipitation uses for mass concentration 95% that volume is concentrate three times ethanol.
As shown in Figure 1, there is becoming for first increases and then decreases with the increase of pH value in mannan-oligosaccharides yield in palm kernel meal
Gesture, when pH value is 11, yield reaches maximum.But after pH value is more than 11, the glycosidic bond of mannan-oligosaccharides can be broken, and be caused
Mannan-oligosaccharides yield declines.In addition, pH value 11, compared with pH value 10, the color of extract solution is deeper, and this is probably body in extraction process
System occurs caused by Maillard reaction.Consider, pH value suitably selects 10.
Embodiment 3
The method that a kind of low alkali of microwave radiation technology produces mannan-oligosaccharides from palm kernel meal：
Step 1)：Palm kernel meal pre-treatment：Palm kernel meal is crushed into fineness to sieve for 200 mesh；
Step 2)：By the palm kernel meal after crushing by palm kernel meal: water=1: after 5 add water to stir, Microwave-assisted Extraction
Take, the frequency of microwave is 2500mHz, and pH value is adjusted into 10 respectively using saturation sodium hydroxide solution, respectively handle 6min,
8min、10min、12min、14min；
Step 3)：After mixed liquor press filtration or centrifugation that step 2) is obtained, supernatant is taken, its pH value is adjusted to 7, use is sweet
Dew dextranase is concentrated in vacuo after digesting 8 hours；
Step 4)：The concentrate that step 3) is obtained is centrifuged after alcohol precipitation, and obtained sediment is freeze-dried, and is produced
To mannan-oligosaccharides crude product, the alcohol that middle alcohol precipitation uses for mass concentration 95% that volume is concentrate three times ethanol.
As shown in Figure 2, mannan-oligosaccharides yield can gradually increase with the increase of microwave treatment time in palm kernel meal,
Reach maximum in 10-14min；Continue to increase processing time, mannan-oligosaccharides yield can without much changes, or even under
Drop.Therefore, microwave treatment time suitably selects 10-14min to be advisable.
By high performance liquid chromatography, compared with mannose, mannan-oligosaccharides standard items, detect whether it is that sweet dew is low
Glycan, and its content, result of the test is as Figure 1-3.
Fig. 2, the yield calculating process of mannan-oligosaccharides shown in 3 are shown below：
Yield=(mannan-oligosaccharides content/palm kernel meal total amount of detection) × 100%.
Claims (5)
1. the method that a kind of low alkali of microwave radiation technology produces mannan-oligosaccharides from palm kernel meal, it is characterised in that including following step
Suddenly：
Step 1)：Palm kernel meal pre-treatment：Palm kernel meal is crushed into fineness to sieve for 50-400 mesh；
Step 2)：By the palm kernel meal after crushing add water stir after microwave radiation exaraction, pH value is adjusted to by 8- using alkali lye
13,6-14 is more than minute for processing；
Step 3)：After mixed liquor press filtration or centrifugation that step 2) is obtained, supernatant is taken, its pH value is adjusted to 7, gathered using sweet dew
Carbohydrase is concentrated in vacuo after enzymolysis 7-12 hours；
Step 4)：The concentrate that step 3) is obtained is centrifuged after alcohol precipitation, and obtained sediment is freeze-dried, that is, is obtained sweet
Reveal oligosaccharide crude product.
2. the method that the low alkali of microwave radiation technology as claimed in claim 1 produces mannan-oligosaccharides from palm kernel meal, its feature exist
In the frequency of microwave is 2450mHz ± 50Hz in the step 2).
3. the method that the low alkali of microwave radiation technology as claimed in claim 1 produces mannan-oligosaccharides from palm kernel meal, its feature exist
In the weight of palm kernel meal and water ratio is 1: 5 in the step 2).
4. the method that the low alkali of microwave radiation technology as claimed in claim 1 produces mannan-oligosaccharides from palm kernel meal, its feature exist
In pH value is adjusted to 10 during microwave radiation exaraction microwave radiation exaraction in the step 2), handles 10-14 minutes.
5. the method that the low alkali of microwave radiation technology as claimed in claim 1 produces mannan-oligosaccharides from palm kernel meal, its feature exist
In for the alcohol that alcohol precipitation uses in the step 4) for the ethanol of mass concentration 95%, its volumetric usage is 3 times of concentrate.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108359701A (en) * | 2018-02-28 | 2018-08-03 | 浙江天草生物科技股份有限公司 | A method of mannan-oligosaccharides being prepared from coffee bean with enzymatic isolation method |
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2017
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Cited By (1)
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| CN108359701A (en) * | 2018-02-28 | 2018-08-03 | 浙江天草生物科技股份有限公司 | A method of mannan-oligosaccharides being prepared from coffee bean with enzymatic isolation method |
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Effective date of registration: 20180822 Address after: 201516 No. 6856, Cao Lang Road, Jinshan District, Shanghai. Applicant after: Shanghai Lamp Biological Science & Technology Co., Ltd. Address before: 215000 1 Changtai Road, Huang Dai Town, Xiangcheng District, Suzhou, Jiangsu. Applicant before: Suzhou win Biotechnology Co., Ltd. |
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Application publication date: 20171212 |