CN107459570A - A kind of functional protein peptide product - Google Patents
A kind of functional protein peptide product Download PDFInfo
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- CN107459570A CN107459570A CN201710562318.2A CN201710562318A CN107459570A CN 107459570 A CN107459570 A CN 107459570A CN 201710562318 A CN201710562318 A CN 201710562318A CN 107459570 A CN107459570 A CN 107459570A
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Abstract
The invention discloses a kind of functional protein peptide product, amino acid sequence SCASRCKSRCRARRCRARCGYFGKRCICKCFRC.Preparation process is：1）Chymotryptic；2）Papain enzymolysis；3）Isoelectric precipitation；4）Decoloration deodorization；5）Purifying.Have the beneficial effect that：Digested using stepwise discretization method, the enzymolysis efficiency of protease is high, reduces cost, successively using chymotrypsin and papain enzymolysis, obtains a large amount of small proteins and polypeptide；Obtained functional protein peptide has repair to beta Cell of islet, promotes insulin structure genetic transcription to form the messenger RNA of preproinsulin（mRNA）, the insulin synthesis in the ribosome of endoplasmic reticulum.Therefore, above-mentioned functional protein peptide product has preferable prevention effect to diabetes.
Description
Technical field
The present invention relates to bioprotein technical field, specifically a kind of preparation method of functional protein peptide product.
Background technology
With society and expanding economy, the health perception of people is more and more stronger, also more next to the quality nutrient of food
More pay close attention to and pay attention to.Biologically active peptide is derived from the multi-functional compounds of protein, and important regulation is played in vital movement
Effect, absorption easy to digest, there are immune promotion, hormone control, antibacterial, antiviral, anti-oxidant, lowering blood pressure and blood fat, hypoglycemic etc.
Effect, edible safety is high, is the function factor of the most popular research topic of current international food circle and great development prospect.
At present, functional polypeptide research has been included in the high-technology field of state key support.
Crucian（Scientific name：Carassius auratus, abbreviation crucian carp, popular name crucian carp, the moon young, the native crucian carp of crucian carp, thin head, shad,
Cold crucian carp）It is common fresh-water fishes in In Eurasia, for the one of which fish of spoke fin net-rope Cypriniformes Cyprinidae crucian carp category.Crucian is mainly
Omnivorefish using plant as food, happiness is gregarious and goes, and selects food and occupies.Fine and tender taste, meat nutritive value is very high, contains egg per hectogram meat
13 grams of white matter, 11 grams of fat, and contain the mineral matters such as substantial amounts of calcium, phosphorus, iron.Crucian medical value is high, its flat property and sweet taste,
Enter stomach, kidney, have and middle qi-restoratives, except thin, warm stomach feed, bowl spares anger the effect of.Crucian is mainly used in eating, in cooking process
Nutriment in middle crucian is lost in more.And because yield is excessive or volume is too small, there is unsalable phenomenon in crucian, causes resource
Waste.
Functional Polypeptides of the extraction with trophism and physiologically active from native protein, and these Functional Polypeptides are applied to health care
In food and enriched food, it is not only able to comprehensively using the high-quality protein in food and food processing, and can be effective
Raising wide material sources protein raw material added value, while greatly promote the scientific and technological content of traditional food.
Prior art such as Authorization Notice No. is the B of CN 104250285 Chinese invention patent, discloses a kind of large yellow croaker fish
Meat anti-oxidation peptide and purposes, it is characterised in that：The anti-oxidation peptide is pentapeptide compound, amino acid sequence Tyr-Leu-Met-
Ser-Arg, ESI-MS detection provide the Da of molecular weight 651.77（[M+H]+652.45 Da）.The anti-oxidation peptide preparation method
Including being homogenized, digesting, the step such as ultrafiltration and chromatography.Tyr-Leu-Met-Ser-Arg pairs of high activity anti-oxidation peptide being prepared
DPPH free radicals, hydroxyl radical free radical, ABTS free radicals and ultra-oxygen anion free radical have good scavenging action.Above-mentioned antioxygen
It is longer to change peptide product preparation time, has fishy smell and in faint yellow, product quality has much room for improvement.
The content of the invention
It is an object of the invention to provide one kind without fishy smell, white, the feature egg of pancreas excreting insulin can be promoted
The preparation method of white peptide product.
The present invention is directed to the problem of being mentioned in background technology, and the technical scheme taken is：A kind of functional protein peptide product,
Amino acid sequence is SCASRCKSRCRARRCRARCGYFGKRCICKCFRC.
A kind of preparation method of functional protein peptide product, comprises the following steps：
Chymotryptic：Adding chymotrypsin in crucian fish meat emulsion, chymotrypsin addition is 0.1 ~
0.3%, hydrolysis temperature is 45 ~ 55 DEG C, and pH is 7.5 ~ 8.5, and enzymolysis time is 15 ~ 25min.Microwave radiation technology digests, and microwave power is
100 ~ 120W, frequency are 20 ~ 30kHZ.Enzymolysis terminates rear 2 ~ 5min of boiling water bath enzyme deactivations.Microwave radiation technology enzymolysis can significantly shorten albumen
Enzyme enzymolysis time, time cost and cost of labor are reduced, reduce production cost.Chymotrypsin is by sticky flesh of fish meat gruel enzyme
More restriction enzyme sites are exposed, prepared for enzymolysis in next step into viscosity and the less solidliquid mixture of molecular weight by solution；
Papain enzymolysis：Papain is added in enzymolysis liquid, papain addition is 0.2 ~ 0.5%, enzymolysis temperature
Spend for 50 ~ 60 DEG C, pH is 5.0 ~ 6.5, and enzymolysis time is 15 ~ 25min.Microwave radiation technology digests, and microwave power is 100 ~ 120W, frequency
Rate is 20 ~ 30kHZ.Enzymolysis terminates rear enzyme deactivation.Flesh of fish meat gruel is digested using stepwise discretization mode, the enzymolysis effect of protease
Rate is high, reduces cost.The solidliquid mixture that papain obtains to chymotryptic is further digested, crucian
Fish protein molecular weight further reduces, and obtains a large amount of small proteins and polypeptide；
Isoelectric precipitation：Enzymolysis liquid is filtered, takes filtrate, regulation pH value is 2.3 ~ 2.6, centrifuging and taking precipitation.When solution ph is 2.3
When ~ 2.6, solubility rapid decrease of the functional protein peptide in water, protein peptides are separated out in the form of precipitating from solution, albumen
The activity of peptide is not destroyed；
Decoloration deodorization：Add water in gained precipitation, decoloration deodorization agent, stirring, centrifuging and taking supernatant are added after being well mixed；
Purifying：Supernatant is subjected to ultrafiltration and chromatography successively, obtains functional protein peptide, amino acid sequence is：
SCASRCKSRCRARRCRARCGYFGKRCICKCFRC.Obtained functional protein peptide has repair to beta Cell of islet, promotees
Enter the messenger RNA that insulin structure genetic transcription forms preproinsulin（mRNA）, closed in the ribosome of endoplasmic reticulum
Into insulin.Therefore, above-mentioned functional protein peptide product has preferable prevention effect to diabetes.
Preferably, decoloration deodorization agent composition and its parts by weight are：10 ~ 15 parts of bentonite, 4 ~ 9 parts of atlapulgite, alkaline calcium
10 ~ 15 parts of 20 ~ 25 parts of base bentonite and activated carbon.Under physical absorption and chemisorbed double action, functional protein peptide
Fishy smell removes substantially, and percent of decolourization is up to more than 92%, and nitrogen retention rate is more than 85%, and active conservation degree is high.
Preferably, ultrafiltration step is：Supernatant pH is adjusted to 6.0 ~ 6.5, with 1 ~ 1.5kDa of molecular cut off ultrafiltration
Film carries out hyperfiltration treatment, collects molecular weight and is less than 1 ~ 1.5kDa parts.By ultrafiltration, it is miscellaneous more than 1 ~ 1.5kDa to remove molecular weight
Matter, make preliminary purifying to functional protein peptide.
Preferably, chromatographic step is：Ultrafiltrate is made into 20 ~ 30mg/mL solution with distilled water, handed over by anion
Resin separation is changed, is eluted with distilled water, 0.45 ~ 0.55 mol/L and 0.90 ~ 1.10 mol/L NaCl solutions, root
Elution fraction is collected according to the absorbance curve under 280 nm, and is purified using reversed-phase liquid chromatography, obtains functional protein
Peptide.Functional protein peptide is further purified chromatographic runs, obtains high-purity functional protein peptide.
Compared with prior art, the advantage of the invention is that：1）The present invention is digested using microwave radiation technology, microwave radiation technology enzymolysis
The protease hydrolyzed time can significantly be shortened, reduce time cost and cost of labor, reduce production cost；2）The present invention is using substep
Enzymatic isolation method digests, and the enzymolysis efficiency of protease is high, reduces cost, successively using chymotrypsin and papain enzymolysis,
Obtain a large amount of small proteins and polypeptide；3）Obtained functional protein peptide has repair to beta Cell of islet, promotes pancreas islet
Plain structural gene transcribes the messenger RNA to form preproinsulin（mRNA）, pancreas islet is synthesized in the ribosome of endoplasmic reticulum
Element.Therefore, above-mentioned functional protein peptide product has preferable prevention effect to diabetes.
Specific embodiment
The present invention program is described further below by embodiment：
Embodiment 1：
A kind of functional protein peptide product, amino acid sequence SCASRCKSRCRARRCRARCGYFGKRCICKCFRC.
A kind of preparation method of functional protein peptide product, comprises the following steps：
1）Chymotryptic：Chymotrypsin is added in crucian fish meat emulsion, chymotrypsin addition is 0.1
~ 0.3%, hydrolysis temperature is 45 ~ 55 DEG C, and pH is 7.5 ~ 8.5, and enzymolysis time is 15 ~ 25min.Microwave radiation technology digests, microwave power
For 100 ~ 120W, frequency is 20 ~ 30kHZ.Enzymolysis terminates rear 2 ~ 5min of boiling water bath enzyme deactivations.Microwave radiation technology enzymolysis can significantly shorten egg
White enzyme enzymolysis time, reduces time cost and cost of labor, reduces production cost.Chymotrypsin is by sticky flesh of fish meat gruel
Viscosity and the less solidliquid mixture of molecular weight are digested into, more restriction enzyme sites are exposed, it is valid to digest in next step
It is standby；
2）Papain enzymolysis：Papain is added in enzymolysis liquid, papain addition is 0.2 ~ 0.5%, enzymolysis
Temperature is 50 ~ 60 DEG C, and pH is 5.0 ~ 6.5, and enzymolysis time is 15 ~ 25min.Microwave radiation technology digests, and microwave power is 100 ~ 120W,
Frequency is 20 ~ 30kHZ.Enzymolysis terminates rear enzyme deactivation.Flesh of fish meat gruel is digested using stepwise discretization mode, the enzymolysis of protease
Efficiency high, reduce cost.The solidliquid mixture that papain obtains to chymotryptic is further digested, crucian carp
Fish fish protein molecular weight further reduces, and obtains a large amount of small proteins and polypeptide；
3）Isoelectric precipitation：Enzymolysis liquid is filtered, takes filtrate, regulation pH value is 2.3 ~ 2.6, centrifuging and taking precipitation.When solution ph is
When 2.3 ~ 2.6, solubility rapid decrease of the functional protein peptide in water, protein peptides are separated out in the form of precipitating from solution,
The activity of protein peptides is not destroyed；
4）Decoloration deodorization：Add water in gained precipitation, decoloration deodorization agent, stirring, centrifuging and taking supernatant are added after being well mixed；
5）Purifying：Supernatant is subjected to ultrafiltration and chromatography successively, obtains functional protein peptide, amino acid sequence is：
SCASRCKSRCRARRCRARCGYFGKRCICKCFRC.Obtained functional protein peptide has repair to beta Cell of islet, promotees
Enter the messenger RNA that insulin structure genetic transcription forms preproinsulin（mRNA）, closed in the ribosome of endoplasmic reticulum
Into insulin.Therefore, above-mentioned functional protein peptide product has preferable prevention effect to diabetes.
Decoloration deodorization agent composition and its parts by weight are：10 ~ 15 parts of bentonite, 4 ~ 9 parts of atlapulgite, alkaline calcium bentonite 20
~ 25 parts and 10 ~ 15 parts of activated carbon.Under physical absorption and chemisorbed double action, the fishy smell of functional protein peptide is gone substantially
Remove, percent of decolourization is up to more than 92%, and nitrogen retention rate is more than 85%, and active conservation degree is high.
Ultrafiltration step is：Supernatant pH is adjusted to 6.0 ~ 6.5, ultrafiltration is carried out with 1 ~ 1.5kDa of molecular cut off milipore filter
Processing, collect molecular weight and be less than 1 ~ 1.5kDa parts.By ultrafiltration, the impurity that molecular weight is more than 1 ~ 1.5kDa is removed, to function
Property protein peptides make preliminary purifying.
Chromatographic step is：Ultrafiltrate is made into 20 ~ 30mg/mL solution with distilled water, by anion exchange resin point
From being eluted with distilled water, 0.45 ~ 0.55 mol/L and 0.90 ~ 1.10 mol/L NaCl solutions, according to 280 nm
Under absorbance curve collect elution fraction, and purified using reversed-phase liquid chromatography, obtain functional protein peptide.Chromatography behaviour
Functional protein peptide is further purified work, obtains high-purity functional protein peptide.
Embodiment 2：
A kind of functional protein peptide product, amino acid sequence SCASRCKSRCRARRCRARCGYFGKRCICKCFRC.
A kind of preparation method of functional protein peptide product, comprises the following steps：
1）Chymotryptic：Chymotrypsin is added in crucian fish meat emulsion, chymotrypsin addition is
0.2%, hydrolysis temperature is 50 DEG C, pH 8.0, enzymolysis time 20min.Microwave radiation technology digests, microwave power 110W, frequency
For 25kHZ.Enzymolysis terminates rear boiling water bath 4min enzyme deactivations.Microwave radiation technology enzymolysis can significantly shorten the protease hydrolyzed time, during reduction
Between cost and cost of labor, reduce production cost.Chymotrypsin digests sticky flesh of fish meat gruel into viscosity and molecular weight
Less solidliquid mixture, more restriction enzyme sites are exposed, prepared for enzymolysis in next step；
2）Papain enzymolysis：Papain is added in enzymolysis liquid, papain addition is 0.4%, hydrolysis temperature
For 55 DEG C, pH 5.4, enzymolysis time 20min.Microwave radiation technology digests, microwave power 110W, frequency 25kHZ.Enzymolysis knot
Enzyme deactivation after beam.Flesh of fish meat gruel is digested using stepwise discretization mode, the enzymolysis efficiency of protease is high, reduces cost.Pawpaw
The solidliquid mixture that protease obtains to chymotryptic is further digested, and crucian fish protein molecular weight enters one
Step reduces, and obtains a large amount of small proteins and polypeptide；
3）Isoelectric precipitation：Enzymolysis liquid is filtered, takes filtrate, regulation pH value is 2.5, centrifuging and taking precipitation.When solution ph is 2.5
When, solubility rapid decrease of the functional protein peptide in water, protein peptides are separated out in the form of precipitating from solution, protein peptides
Activity is not destroyed；
4）Decoloration deodorization：Add water in gained precipitation, decoloration deodorization agent, stirring, centrifuging and taking supernatant are added after being well mixed；
5）Purifying：Supernatant is subjected to ultrafiltration and chromatography successively, obtains functional protein peptide, amino acid sequence is：
SCASRCKSRCRARRCRARCGYFGKRCICKCFRC.Obtained functional protein peptide has repair to beta Cell of islet, promotees
Enter the messenger RNA that insulin structure genetic transcription forms preproinsulin（mRNA）, closed in the ribosome of endoplasmic reticulum
Into insulin.Therefore, above-mentioned functional protein peptide product has preferable prevention effect to diabetes.
Decoloration deodorization agent composition and its parts by weight are：13 parts of bentonite, 5 parts of atlapulgite, 23 parts of alkaline calcium bentonite and
13 parts of activated carbon.Under physical absorption and chemisorbed double action, the fishy smell of functional protein peptide removes substantially, and percent of decolourization reaches
93%, nitrogen retention rate is 86%, and active conservation degree is high.
Ultrafiltration step is：Supernatant pH is adjusted to 6.3, hyperfiltration treatment is carried out with molecular cut off 1.2kDa milipore filter,
Collect molecular weight and be less than 1.2kDa parts.By ultrafiltration, the impurity that molecular weight is more than 1.2kDa is removed, functional protein peptide is made
Preliminary purifying.
Chromatographic step is：Ultrafiltrate is made into 24mg/mL solution with distilled water, by Anion exchange resin separation,
Eluted with distilled water, 0.5mol/L and 1mol/L NaCl solutions, elution group is collected according to the absorbance curve under 280 nm
Point, and purified using reversed-phase liquid chromatography, obtain functional protein peptide.Chromatographic runs are further pure to functional protein peptide
Change, obtain high-purity functional protein peptide.
Embodiment 3：
A kind of functional protein peptide product, amino acid sequence SCASRCKSRCRARRCRARCGYFGKRCICKCFRC.
A kind of preparation method of functional protein peptide product, comprises the following steps：
1）Chymotryptic：Chymotrypsin is added in crucian fish meat emulsion, chymotrypsin addition is
0.3%, hydrolysis temperature is 50 DEG C, pH 8.0, enzymolysis time 16min.Microwave radiation technology digests, microwave power 110W, frequency
For 25kHZ.Enzymolysis terminates rear boiling water bath 4min enzyme deactivations.Microwave radiation technology enzymolysis can significantly shorten the protease hydrolyzed time, during reduction
Between cost and cost of labor, reduce production cost.Chymotrypsin digests sticky flesh of fish meat gruel into viscosity and molecular weight
Less solidliquid mixture, more restriction enzyme sites are exposed, prepared for enzymolysis in next step；
2）Papain enzymolysis：Papain is added in enzymolysis liquid, papain addition is 0.5%, hydrolysis temperature
For 55 DEG C, pH 5.4, enzymolysis time 15min.Microwave radiation technology digests, microwave power 110W, frequency 25kHZ.Enzymolysis knot
Enzyme deactivation after beam.Flesh of fish meat gruel is digested using stepwise discretization mode, the enzymolysis efficiency of protease is high, reduces cost.Pawpaw
The solidliquid mixture that protease obtains to chymotryptic is further digested, and crucian fish protein molecular weight enters one
Step reduces, and obtains a large amount of small proteins and polypeptide；
3）Isoelectric precipitation：Enzymolysis liquid is filtered, takes filtrate, regulation pH value is 2.5, centrifuging and taking precipitation.When solution ph is 2.5
When, solubility rapid decrease of the functional protein peptide in water, protein peptides are separated out in the form of precipitating from solution, protein peptides
Activity is not destroyed；
4）Decoloration deodorization：Add water in gained precipitation, decoloration deodorization agent, stirring, centrifuging and taking supernatant are added after being well mixed；
5）Purifying：Supernatant is subjected to ultrafiltration and chromatography successively, obtains functional protein peptide, amino acid sequence is：
SCASRCKSRCRARRCRARCGYFGKRCICKCFRC.Obtained functional protein peptide has repair to beta Cell of islet, promotees
Enter the messenger RNA that insulin structure genetic transcription forms preproinsulin（mRNA）, closed in the ribosome of endoplasmic reticulum
Into insulin.Therefore, above-mentioned functional protein peptide product has preferable prevention effect to diabetes.
Decoloration deodorization agent composition and its parts by weight are：14 parts of bentonite, 4 parts of atlapulgite, 25 parts of alkaline calcium bentonite and
12 parts of activated carbon.Under physical absorption and chemisorbed double action, the fishy smell of functional protein peptide removes substantially, and percent of decolourization reaches
93.4%, nitrogen retention rate is 85.7%, and active conservation degree is high.
Ultrafiltration step is：Supernatant pH is adjusted to 6.3, hyperfiltration treatment is carried out with molecular cut off 1.2kDa milipore filter,
Collect molecular weight and be less than 1.2kDa parts.By ultrafiltration, the impurity that molecular weight is more than 1.2kDa is removed, functional protein peptide is made
Preliminary purifying.
Chromatographic step is：Ultrafiltrate is made into 24mg/mL solution with distilled water, by Anion exchange resin separation,
Eluted with distilled water, 0.5mol/L and 1mol/L NaCl solutions, elution group is collected according to the absorbance curve under 280 nm
Point, and purified using reversed-phase liquid chromatography, obtain functional protein peptide.Chromatographic runs are further pure to functional protein peptide
Change, obtain high-purity functional protein peptide.
Embodiment 4：
Selecting blood glucose value, 25 diabetics are as test group for random selection, allow for 12.0 ~ 14.0 50 diabetics
The functional protein peptide product that its each one after each meal 0.2g embodiment 3 obtains, 25 diabetics are as a control group in addition
Do not take, and control the diet of 50 diabetics identical.By the experiment of 60 days, every patient of diabetes of test group is recorded
The frequency of injection of person's insulin, and its average value is taken, the frequency of injection of every diabetic's insulin of control group is recorded, and take
Its average value.
The frequency of injection of the diabetic's insulin of table 1
| Group name | Control group | Test group |
| Insulin mean injection frequency times/day | 1.8 | 0.6 |
As seen from Table 1, functional protein peptide of the present invention has prevention effect to diabetes, can effectively mitigate diabetic symptom, tool
There are good market prospects.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
SEQUENCE LISTING
<110>Zhejiang Feng Yu marine organisms Products Co., Ltd
<120>A kind of functional protein peptide product
<130> 1
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 33
<212> PRT
<213>It is artificial synthesized
<400> 1
Ser Cys Ala Ser Arg Cys Lys Ser Arg Cys Arg Ala Arg Arg Cys Arg
1 5 10 15
Ala Arg Cys Gly Tyr Phe Gly Lys Arg Cys Ile Cys Lys Cys Phe Arg
20 25 30
Cys
Claims (8)
- A kind of 1. functional protein peptide product, it is characterised in that：The amino acid sequence of described functional protein peptide is SCASRCKSRCRARRCRARCGYFGKRCICKCFRC。
- A kind of 2. functional protein peptide product according to claim 1, it is characterised in that：Described functional protein peptide production The preparation process of product includes chymotryptic, papain enzymolysis, isoelectric precipitation, decoloration deodorization and purifying.
- A kind of 3. preparation method of functional protein peptide product according to claim 2, it is characterised in that：Described pancreas coagulates For galactase enzymolysis step to add chymotrypsin, microwave radiation technology enzymolysis in crucian fish meat emulsion, enzymolysis terminates rear enzyme deactivation； Chymotrypsin addition be 0.1 ~ 0.3%, hydrolysis temperature be 45 ~ 55 DEG C, pH be 7.5 ~ 8.5, enzymolysis time be 15 ~ 25min；Microwave power is 100 ~ 120W, and frequency is 20 ~ 30kHZ.
- A kind of 4. preparation method of functional protein peptide product according to claim 2, it is characterised in that：Described pawpaw For protease hydrolyzed step to add papain, microwave radiation technology enzymolysis in enzymolysis liquid, enzymolysis terminates rear enzyme deactivation；Papain Enzyme addition is 0.2 ~ 0.5%, and hydrolysis temperature is 50 ~ 60 DEG C, and pH is 5.0 ~ 6.5, and enzymolysis time is 15 ~ 25min；Microwave power For 100 ~ 120W, frequency is 20 ~ 30kHZ.
- A kind of 5. preparation method of functional protein peptide product according to claim 2, it is characterised in that：Described waits electricity Point settling step is filtering enzymolysis liquid, takes filtrate, and regulation pH value is 2.3 ~ 2.6, centrifuging and taking precipitation.
- A kind of 6. preparation method of functional protein peptide product according to claim 2, it is characterised in that：Described decolouring Deodorant step is in gained precipitation plus water, and decoloration deodorization agent, stirring, centrifuging and taking supernatant are added after being well mixed；Decoloration deodorization Agent composition and its parts by weight are：10 ~ 15 parts of bentonite, 4 ~ 9 parts of atlapulgite, 20 ~ 25 parts of alkaline calcium bentonite and activated carbon 10 ~ 15 parts.
- A kind of 7. preparation method of functional protein peptide product according to claim 2, it is characterised in that：Described purifying Step includes ultrafiltration：Supernatant pH is adjusted to 6.0 ~ 6.5, hyperfiltration treatment is carried out with 1 ~ 1.5kDa of molecular cut off milipore filter, Collect molecular weight and be less than 1 ~ 1.5kDa parts.
- A kind of 8. preparation method of functional protein peptide product according to claim 2, it is characterised in that：Described is pure Changing step includes chromatography：Ultrafiltrate is made into 20 ~ 30mg/mL solution with distilled water, by Anion exchange resin separation, used Distilled water, 0.45 ~ 0.55 mol/L and 0.90 ~ 1.10 mol/L NaCl solutions are eluted, according to the suction under 280 nm Luminosity curve collects elution fraction, and is purified using reversed-phase liquid chromatography, obtains functional protein peptide.
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