CN107455042A - A kind of river creeping weed seed drying store method - Google Patents

Abstract

The present invention relates to the store method of seaweed seeds, specifically a kind of river creeping weed seed drying store method.Rinsed well after river creeping weed seed exocarp is rotted completely, the river creeping weed seed rinsed well is directly placed into the sealing container equipped with drier at 5 10 DEG C, is kept in dark place, and then realizes to the preservation under the long-term drying condition of seed；Wherein, ambient humidity is 33% during being kept in dark place, and it is ± 10% to preserve humidity deviation.During Seed storage, the humidity environment in Conservation environment is inspected periodically.Seed storage condition is simple needed for the present invention, and the vigor that seed holding can be made higher and quality are preserved to river creeping weed seed using the method for the present invention, with reference to the river creeping weed Seed storage method that the present invention is simple and effective, the river creeping weed seed of high quality can be provided for artificial seeding, be advantageous to the recovery or reconstruction on underwater vegetation river creeping weed grassland, providing necessary technology for the good river creeping weed Grassland environment of fast quick-recovery supports.

Description

A kind of river creeping weed seed drying store method

Technical field

The present invention relates to the store method of seaweed seeds, specifically a kind of river creeping weed seed drying store method.

Background technology

Sea grass bed (Seagrass meadows) ecosystem and mangrove, coral reef ecologic system are considered as near together Typical marine ecosystems of Hai San great, and international research result shows, sea grass bed Ecosystem Service Value is apparently higher than mangrove With the Ecosystem Service Value of coral reef." sea grass " described here means unique angiosperm being submerged under shallow sea water, differentiation Go out the organs such as root, stem, leaf, flower and seed, it is completely different with " marine alga " (such as sea-tangle, Enteromorpha) as rudimentary plant.It is in great numbers Sea grass bed such as the forest of land, be the important symbol of healthy marine environment, be precious " seabed grassland " or " seabed is gloomy Woods ".Sea grass bed is high productivity area, and good habitat, nursery can be provided for marine organisms such as fish shrimp crab shellfishes and protects ground, It is also beneficial to inhabiting for seabird；Simultaneously important food source is provided for many biologies.Sea grass bed is also maximally effective on the earth Carbon captures and seals one of system up for safekeeping, and the UN organizations think:It is " blue that the most urgent action of reply climate change is maintenance and recovery Carbon " converges, and is given priority to together with sea grass and mangrove, sabkha.

River creeping weed also known as ditch grass, tealgrass.It is under the jurisdiction of bog pondweed mesh (Potamogetonales), river creeping weed section (Ruppiaceae), river creeping weed category (Ruppia).River creeping weed category is a kind of to be widely distributed in whole world river mouth, seashore, wetland Salt water habitat angiosperm submerged macrophytes (i.e. sea grass).In China, river creeping weed distribution south to Hainan Province, north to Liaoning Province (Yu et al.,2014).But the same with other sea grass species, river creeping weed also has the abundant ecological value, such as river creeping weed is food of surviving the winter Careless migratory bird and the important food resource of benthonic invertebrates；Can significantly change water quality of the estuary coast with habitat and The biogeochemical cycle of deposit；And important primary productivity in Coastal Ecosystem.In recent decades, due to being permitted Think that factor is disturbed, river creeping weed vegetation is largely reduced, and its population scope is increasingly reduced more, and the work of protection river creeping weed, which is carved, not to be allowed It is slow.

Because seaweed seeds are typically contained compared with high-moisture, and seed is easy to inactivate after drying condition dehydration；So at present The universal store method of seaweed seeds is that wet method preserves, i.e., seaweed seeds is stored in low temperature, high salinity briny environment.This Sprout though class method can successfully suppress seed and keep seed activity, preservation seed easily bacterial infection and fungi, therefore Need regular, high frequency to change water during Seed storage, take good work.And then it is badly in need of a kind of store method of high-efficient simple, not only It can break away from because bacterium, fungi grow the great damage to seed during river creeping weed seed wet method preserves, but also subtract significantly " the physical cost " during Seed storage is lacked.

The content of the invention

It is an object of the invention to provide a kind of easy river creeping weed seed drying store method.

To achieve the above object, the present invention use technical scheme for：

A kind of river creeping weed seed drying store method, rinsed well after river creeping weed seed exocarp is rotted completely, The river creeping weed seed rinsed well is directly placed into the sealing container equipped with drier at 5-10 DEG C, is kept in dark place, and enters And realize to the preservation under the long-term drying condition of seed；

Wherein, ambient humidity is 33% during being kept in dark place, and it is ± 10% to preserve humidity deviation.

The river creeping weed seed is the ripe river creeping weed seed after exocarp rots completely.

The water for rinsing river creeping weed seed is nature seawater.

The river creeping weed seed is full ripe seed, the i.e. seed with hard black exocarp.

The condition of culture of the river creeping weed seed exocarp putrefaction process is room temperature, lucifuge, salinity 10-15, and is periodically changed Water.

The routine observation river creeping weed Conservation environment humidity, regularly replace the drier in Conservation environment.

The drier is drier commonly used in the art, and such as change colour silica gel.

The beneficial effects of the invention are as follows：

River creeping weed (R.sinensis) seed of the present invention is under the conditions of the kept dry under preference temperature, after its slow dehydration The characteristic of very high activity is remained in that, proposes river creeping weed seed being placed under thermophilic drying condition accordingly and preserves, this method is not only It can break away from because bacterium, fungi grow the great damage to seed during river creeping weed seed wet method preserves, but also subtract significantly " the physical cost " during Seed storage is lacked；Specially：

It is that river creeping weed seed is placed under the drying condition of certain humidity (33 ± 10%), preservation temperature using the inventive method Preserved under the conditions of 5-10 DEG C of degree, lucifuge, reduce " human cost " during preserving, seed can be promoted to keep resting state, Not rudiment, improve the retention rate of river creeping weed seed seed during preservation；Extend the holding time of river creeping weed seed；Reduce The frequency that the creeping weed seed situation that long mycocriny loses during preservation in river occurs；The higher vigor of river creeping weed seed is enough kept simultaneously And quality.The river creeping weed seed of high quality can be provided using the inventive method for artificial seeding, be advantageous to river creeping weed sea grass bed Recover and build, the good ecological environment of fast quick-recovery ocean.

In the preservation of seaweed seeds, it is common that by Seed storage under high salinity, cryogenic conditions.But this kind of method is to sea , it is necessary to which regular, high-frequency is changed to the water body in Seed storage environment during grass seed is preserved, suppressed with this The growth of fungi, bacterium in Conservation environment.It is unexpected with water temperature and what river creeping weed seed wet method preserved changes during water Rise, seed are sprouted valve and easily split, and seed is more easy to during ensuing preservation by bacterium, fungal infection so as to lose Vigor.The present invention has found that river creeping weed seed is under preference temperature under the conditions of the kept dry of (5-10 DEG C), its dehydration based on experiment After remain in that very high activity, propose the river creeping weed seed for taking off exocarp will to be placed directly within thermophilic drying condition accordingly and protect To deposit, this method can not only be broken away from because bacterium, fungi grow the great damage to seed during river creeping weed seed wet method preserves, And " the physical cost " during Seed storage is also greatly reduced, its Heterosis is in following several respects：First, dried strip River creeping weed seed is preserved under part, greatly reduces the requirement to Conservation environment equipment；Secondly, relatively low humidity is passed through Environment is preserved to seed not only ensure that seed has greater activity, further suppress sprouting of the seed during preservation and prolongs The holding time of river creeping weed seed has been grown, has reached the purpose of long-term preservation；Finally, due under drying condition, fungi, bacterium without Method grows, therefore seed is not in corruption during preserving, therefore changes water frequency, pole during reducing river creeping weed Seed storage The earth reduces human cost, reduces the disturbance to preserving seed, avoids germination.In addition, the present invention is in Seed storage During, the drier in Conservation environment need to be regularly replaced, is avoided because ambient humidity is too high, river creeping weed sprouts, specific to dry Agent replacement frequency is depending on container tightness is preserved.

Brief description of the drawings

Fig. 1 is river creeping weed Seed storage method and step flow chart of the present invention.

Embodiment

Below in conjunction with the accompanying drawings to further detailed description of the present invention.

The inventive method not only makes the retention rate of river creeping weed seed seed during preservation high, while ensure that river creeping weed kind Son still has higher sprouting vigor after long-term preserve, and substantially prolongs the holding time of river creeping weed seed.

Store method of the present invention is as shown in Fig. 1 steps：

Ripe river creeping weed (R.sinensis) seed is taken to be placed in the salinity that lucifuge, oxygenation, nature seawater and running water are made into For in 15-20 water body, after its seed exocarp completely corruption, seed is cleaned with nature seawater；

River creeping weed seed is put into the sealed plastic box for filling drier, drier and seed are separated with masking foil；Will After hygrometer is together placed in sealed plastic box, seal box is positioned over 5-10 DEG C, is kept in dark place；

Damp condition in routine observation seal box, regularly replace drier.

Embodiment 1

River creeping weed seed is acquired in the Dongying stretch of coastal water in by the end of September, 2016.River creeping weed seed exocarp putrefaction process Condition of culture is room temperature, lucifuge, salinity 10-15, and is refreshed the water periodically.The seed of experiment is full ripe seed, that is, is had Hard, black exocarp seed.2000 river creeping weed (R.sinensis) mature seeds are randomly selected, impartial is put into humidity For 33 ± 10%, temperature conditionss are 0 DEG C, 5 DEG C, 10 DEG C, 15 DEG C, in 22 DEG C of environment, while add drier.The Seed storage time For 9 months.Every 3 months of period checked ambient humidity situation of change in seal box, and drying is changed for the too high treatment group of humidity Agent.Seed storage is tested after terminating by sprouting, and verifies seed vitality.(rudiment is tested：Taken out at random from each preservation experimental group 200 seeds, it is divided into 3 replicate treatment groups, is placed in environment temperature as 30 DEG C, salinity is to be sprouted in 5 environment).

Wherein, drier is discoloration silica-gel desiccant.

The present invention relates to seed retention rate and seed in seed vitality identification (sprout experiment) germination rate it is specific Data are obtained by testing, and wherein seed vitality identification experiment specific embodiment is：Go bail for and deposit rear 50, river creeping weed seed (3 repetitions), which is placed under the creeping weed seed favourable germination conditions of river, is sprouted (favourable germination conditions：Water salinity is 5, and temperature is 30 DEG C, photoperiod 12L：12D conditions) under quiescent culture 10 days, the germination rate of seed is recorded and calculated.

Seed activity examines seed germination rate computational methods as follows：

Specific sprouting result is as shown in table 1：

River creeping weed seed is preserved under the different temperatures drying condition of table 1, and being averaged for seed is sprouted in seed vitality inspection after 9 months Hair rate (%)

As it can be seen from table 1 after river creeping weed seed drying preserves 9 months, preservation condition is river under the conditions of 5 DEG C, 10 DEG C The average germination rate highest of creeping weed seed, nearly 75%, and it is higher than 0 DEG C (seed germination rate 55.6%) and other Temperature Treatment groups. It can be seen that 5-10 DEG C of environment temperature, ambient humidity is 33 ± 10%, and light protected environment is that suitable river creeping weed seed drying preserves bar Part.

The application of the present invention will be under the premise of river creeping weed seed greater activity be kept, hence it is evident that when improving the preservation of seed Between, with less human cost, realize the foundation of river creeping weed seed bank.

The control of different temperatures keeps steady temperature, product type using low temperature incubator in the present embodiment：KRC- 150CA, manufacturer：Shanghai Qi Xin scientific instrument Co., Ltd；Other temperature and lighting requirements use intelligent illumination box Keep steady temperature and illumination, product type：MGC-250, manufacturer：One permanent Science and Technology Ltd. of Shanghai.

Claims (4)

1. A kind of 1. river creeping weed seed drying store method, it is characterised in that：By it after river creeping weed seed exocarp is rotted completely Rinse well, the river creeping weed seed rinsed well is directly placed into the sealing container equipped with drier at 5-10 DEG C, kept away Light preserves, and then realizes to the preservation under the long-term drying condition of seed；

   Wherein, ambient humidity is 33% during being kept in dark place, and it is+10% to preserve humidity deviation.
2. 2. river creeping weed Seed storage method according to claim 1, it is characterised in that：The river creeping weed seed is exocarp Ripe river creeping weed seed after rotting completely.
3. 3. river creeping weed Seed storage method according to claim 1, it is characterised in that：The water for rinsing river creeping weed seed is certainly Right seawater.
4. 4. river creeping weed Seed storage method according to claim 1, it is characterised in that：Routine observation river creeping weed Conservation environment Humidity, regularly replace the drier in Conservation environment.