CN107441034A - A kind of whitening cosmetics containing five-leaved chaste tree stem cell and preparation method thereof - Google Patents
A kind of whitening cosmetics containing five-leaved chaste tree stem cell and preparation method thereof Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
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Abstract
The invention discloses a kind of whitening cosmetics containing five-leaved chaste tree stem cell,Mainly formed by following weight percent composition,Five-leaved chaste tree stem cell 5~30%,Ginseng extract 2 15%,Prepared rhizome of rehmannia extract solution 2%~15%,Lemon extract 2~15%,It is main material from five-leaved chaste tree stem cell,Activating cell,Remove free radical,Block chloasma,The generation of butterfly macules,Eliminate dermal melanin,Reduce,Remove whelk,So that the pure white beautiful active matter of skin and,There is good compatibility to human body using the stem cell of five-leaved chaste tree,It is easier to be absorbed by the body,Growth to Skin Cell has good facilitation,The metabolism of cell can be accelerated,Strengthen the vigor of Skin Cell,Addition has whitening simultaneously,Skin-active,Enhance metabolism,Strengthen the prepared rehmannia root extract of Skin Cell vigor and other effects,Ginseng extract,Lemon extract,Acted synergistically with five-leaved chaste tree stem cell,Skin quality can be obviously improved,Improve the Reproductive activity of skin,Whitening spot-removing positive effect.
Description
Technical field
The present invention relates to cosmetic technical field, is specifically related to a kind of whitening cosmetics containing five-leaved chaste tree stem cell
And preparation method thereof.
Background technology
It is everyone nature, especially women to like to be beautiful, then as the quickening of people's rhythm of life, operating pressure and life
The increasingly increase of pressure living, and environmental pollution and the careless reason of diet, the growth at simultaneous age, the skin of people
Skin engenders many problems, and such as script is coloured and dazzling on the face, gradually becomes obscure unglazed, colour of skin jaundice, long spot, therefore give
Many women bring puzzlement.
It is well known that skin color is mainly what is determined by the content of melanin in skin, and melanin can protect machine
Injury of the body from ultraviolet.Melanin is synthesized by melanocyte.Melanin and protein combination shape in melanocyte
Into melanosome, at this moment tyrosine is transformed into eumelanin or pheomelanin by tyrosinase.After melanosome is discharged from melanocyte
Skin surface is transported to by horn cell, and as a part for horn cell.The depth of skin color is formed with melanin
The amount for measuring and being transferred to the melanin in horn cell is directly proportional, and under ultraviolet irradiation, horn cell can discharge one
The kind basic element of cell division, referred to as Endothelin, after it is received by the acceptor of melanocyte, meeting stimulation melanin cell propagation,
Differentiation, and the activity of tyrosinase is activated, improve the synthetic quantity of melanin；
A kind of skin phenomena of the common face with people of color spot, belongs to dyschromicum skin disease, and color spot includes freckle, black
Spot, chloasma and senile plaque expelling etc., chloasma are a kind of pigment proliferative skin disorders for betiding face, are mainly in young and middle-aged female
Property；Clinic proves that the formation of color spot is broadly divided into internal cause and external cause, and internal cause has with endocrinopathy, personality mood, physiologic factor
Close relation, external cause mainly with we usually using not when the cosmetics of oneself the either product of inferior quality or in ultraviolet
Under acute irradiation, do not properly protect and arrange
Shi Douhui causes the formation of color spot.
The product of whitening spot-removing is many at present, but major part uses chemical synthesis agent, and some products are even containing to human body
Harmful lead mercury part, using can be caused damage too long to skin, such as quinhydrones, hydrogen peroxide, carbolic acid class preparation, there is corruption
Corrosion, and easily recur.Some products then only serve covering effect in using effect, without the color spot removal effect that disappears.Not only rise not
To cosmetology effect, easily aggravate color spot on the contrary, xerosis cutis are coarse, and pore is thick, and people recognize one after another overcomes this
One possible direction of a little shortcomings is using the active material extracted from natural goods, utilizes the various composition in mixture
Synergy mechanism suppresses the purpose of tyrosinase and peroxidase to reach, and suppresses melanin generation in joint fashion.
Therefore, compound is extracted from natural products and is used for whitening and freckle-removing product into the study hotspot of cosmetics.However, mostly
Number product, although being improved to natural extract component on composition, function is single, whitening spot-removing DeGrain, therefore needs
A safely and effectively whitening spot-removing skin-protection product is wanted, will can solve this skin care problem that current most of women face.
The content of the invention
A kind of whitening cosmetics and preparation method thereof are provided it is an object of the invention to overcome above mentioned problem, the U.S.
The cause of disease and the interpretation of the cause, onset and process of an illness that white spot-eliminating composition is formed for color spots such as the obscure, chloasma of the colour of skin, utilize the work of plurality of Chinese extract
The mutual synergy of property composition, is effectively reduced melanin over-deposit in skin, easily eliminates color spot.
To achieve the above object, the present invention adopts the technical scheme that, a kind of whitening cosmetics, contains following quality
The component of percentage composition, five-leaved chaste tree stem cell 5~30%, Ginseng extract 2~15%, prepared rhizome of rehmannia extract solution 2%~15%, lemon
Extract solution 2~15%.
Preferably, in addition to NMF 15~20%, emulsifying agent 4~8%, preservative 0~0.5%, grease 1.5%~
3.5%, triethanolamine 0.3~0.6%, surplus forms for deionized water；
Described thickener is from the one or more combination referred in card ripple 980, xanthan gum, esters of acrylic acid.
The NMF is from one or more of groups in micromolecule hyaluronic acid sodium, trehalose, glucan, tremella polysaccharides
Close.
Described emulsifying agent from lauryl sodium sulfate, AEO, one kind in oliver1000 or
Several combinations.
The preservative is from one kind in Phenoxyethanol, potassium sorbate.
Preferably, the five-leaved chaste tree stem cell 10~30%, Ginseng extract 8~15%, prepared rhizome of rehmannia extract solution 6%~
15%th, lemon extract 6~10%.
Preferably, the five-leaved chaste tree stem cell 20%, Ginseng extract 15%, prepared rhizome of rehmannia extract solution 10%, lemon extract
10%.
Preferably, the five-leaved chaste tree stem cell 30%, Ginseng extract 13%, prepared rhizome of rehmannia extract solution 15%, lemon extract
8%.
Preferably, it is characterised in that：Comprise the following steps：
1) preparation of five-leaved chaste tree stem cell：A) after the new branch sterilization of five-leaved chaste tree, xylem and marrow being removed, it is inoculated in Fiber differentiation
Base, inducing culture obtain cambial cell；B) cambial cell is trained through culture medium through subculture, access proliferated culture medium, shaking table
It is unicellular to support acquisition；C) the unicellular proliferated culture medium that is inoculated in is cultivated through expanding, and obtains five-leaved chaste tree stem cell；
2) preparation of plant extraction liquid：The weight of raw material is weighed into ginseng, prepared rhizome of rehmannia, lemon, mechanical crushing is
The particle of 0.2~0.5cm particle diameters, 60% ethanol of 10 times of weight is added, is soaked 10 hours under normal temperature, immersion is twice.Extracting
Active carbon powder is added in liquid, stirring is put into 5 degrees Celsius of environment after 5~10 minutes and stands 10~20 hours, and supernatant crosses 120
Mesh sieve, filtrate is concentrated under reduced pressure under 35 degrees Celsius, is condensed into the equiponderant Chinese medicine material medicine with initiation material medicine
Alcohol extracting thing concentrate, it is standby；
3) in the state of stirring, thickener, emulsifying agent are slowly added into deionized water, heating stirring to 80-90
DEG C, it is incubated to complete swelling；
4) to NMF is added in 3), stirring is continued to after dissolving completely, the ￣ 4min of homogeneous 2；
5) grease is added to the mixture in 4), after 3~5min, insulation 10-15min is complete to defoaming, and treats that temperature is down to
During 55 60 DEG C of ￣ or so, add appropriate triethanolamine and be neutralized to pH to 6.0 ￣ 7.0 or so, stir；
6) 40~45 DEG C are down to the mass temperature of 5) middle gained, add five-leaved chaste tree stem cell, Ginseng extract, prepared rhizome of rehmannia and carry
Liquid, lemon extract, root of Dahurain angelica extract solution, pollen pini, evening primrose oil, millet starch fruit extract solution, meadow pine extract solution, preservative are taken,
Stir, be cooled to 36 DEG C, stop stirring, discharging；
The inducing culture is the MS solid mediums containing NAA, BA and caseinhydrolysate, and the proliferated culture medium is
MS fluid nutrient mediums containing 2,4-D, BA, caseinhydrolysate and activated carbon.
Preferably, the raw material in the step 2) also includes the root of Dahurain angelica；Also include adding in the step 6) root of Dahurain angelica extract solution,
Pollen pini.
Whitening spot-removing main component and effect are as follows used in the present invention：
Five-leaved chaste tree：Contain flavone compound：Vitex negundo, cyanidenon -7- glycosides, artemetine, orientin, different Polygonum
Careless element.Iridoid glycosides：Aucubin, pale purple colored vitexin.Organic acid：P-hydroxybenzoic acid, 5- hydroxyls isophthalic two
Acid, PCA, protocatechuic acid, vitamin C, 5- oxygen-different phthalein.Alkaloids：Buddhist nun's ginseng determines alkali.
Flavone compound can suppress tyrosinase, and flavonols is the competitive inhibitor of tyrosinase, flavonoids
With protein recombination reaction occurs in a manner of hydrophobic bond and hydrogen bond for compound, thus the skin of people is produced convergence so that is added with
The cosmetics of five-leaved chaste tree have good adhewsive action under waterproof condition to skin, and can shrink thick pore, make relaxation
Skin contraction, collapse tight, there is very strong removing free radical and anti-lipid peroxidation characteristic, vitamin C for skin, bone,
Blood vessel, pressure etc. have the effect of very strong, and health can be improved by taking vitamin C, from metabolism to the prevention of disease and control
Treat, there is sizable help.Vitamin C, which has, helps folic acid and mineral metabolism, absorbs glucose and amino acid；Improve white
Blood cell function, improve immunity, preventing cold；Skin and bone collagen generation are helped, maintains bone density and other effects, institute
Effect containing organic acid is extensive, including the effect such as anti-inflammatory, anti-oxidant, anticancer, liver protection, immunological regulation, antibacterial, antiviral.
The present invention is raw material from the stem cell of five-leaved chaste tree, and plant stem cell includes on development of plants and grown all
Formula, possess the cell of eternal life power, be the root (origin) of plant life power.Plant stem cell, which is present in, to be referred to as point
In the special tectonic of raw tissue, there is very surprising power of regeneration.These allow plant constantly to be grown between centuries,
And generate brand-new organ.Plant stem cell is the primary raw material and source of skin progenitor cell metabolism, can quickly be activated not
Dormancy stem cell, promote stem cell self-regeneration renewal, the first viability of reduction epidermal stem cells, make Skin Cell newborn in a steady stream, from
And reach lifting, firm skin, desalinate the anti-aging effects of wrinkle, while can be easier to be absorbed by the skin the nutrition of plant into
Point；Accelerate human metabolism.
Ginseng：Ginseng contains ginsenoside, organic acid and esters, microorganism (vitamin (Vitamine) B1, vitamin
B2, vitamin B12, vitamin C) etc. active ingredient, its panaxoside can promote the blood circulation of subcutaneous capillary, increase skin
The nutrition supply of skin, prevent the effect such as artery sclerosis, regulation moisture of skin balance.Possesses the composition of astriction in panaxoside
It is more, also there is contractive effect to skin, and corium water balance can be adjusted, skin dehydration is prevented, the original of dark black element can be restrained again
Performance makes skin become smooth, tender, pure white.So its energy delay skin aging, increases the elasticity of skin, is protected so as to play
Skin gloss is tender, prevents and cut down the effect of wrinkle of skin, and ginseng active material is also equipped with restraining the reducing property of dark black element,
Make the pure white smooth ginseng active material of skin that also there is the reducing property for suppressing melanin, make skin pure white smooth.It contains simultaneously
Some vitamin Cs and B family vitamin coordinative role, can activating cell, remove free radical, block chloasma, butterfly macules production
It is raw, dermal melanin is eliminated, reduces, remove whelk so that the pure white beauty of skin；Its beauty effectiveness is innumerable.
Prepared rhizome of rehmannia：Prepared rhizome of rehmannia is goatweed, chemical composition based on glucoside, wherein again using iridoid glycosides as
It is main, mainly include motherwort glucoside, Aucubin, Catalpol, ground common glucoside A, B, C, D etc., also containing various alcohol compounds, list
Saccharide compound, several amino acids, a variety of inorganic elements such as sugar and polysaccharide etc..With whitening, skin-active, enhance metabolism, increase
Strong Skin Cell vigor and other effects, make face glossy, prevention is prematurely senile.
Lemon：Containing carbohydrate, citric acid, malic acid, hesperidine, naringin, vitamin B1 .B2.C, nicotinic acid, calcium, phosphorus, iron etc.
Composition, there is dispelling fatigue, improve muscular work ability, enhance metabolism, anti-aging phenomenon and activating skin epidermis
The various health care functions such as cell.And its unique citric acid composition not only can prevent and eliminate calmness of the pigment in skin more
Can cutin-softening layer, make skin become whitening and rich in gloss.
Trehalose：Diaphragm is formed in the cell in high temperature, high and cold, hyperosmosis and in the case of drying dehydration, effectively
Protected protein matter non-inactivation is denatured, and so as to the life process and vital signs of the body that sustains life, is applied to this change as NMF
In cosmetic.
Beneficial effects of the present invention：The present invention is main material from five-leaved chaste tree stem cell, utilizes the active ingredient of five-leaved chaste tree, energy
Enough activating cells, remove free radical, block the generation of chloasma, butterfly macules, eliminate dermal melanin, reduce, remove whelk,
So that the pure white beautiful active matter of skin and, there is good compatibility to human body using the stem cell of five-leaved chaste tree, it is easier to by human body
Absorb, the growth to Skin Cell has good facilitation, can accelerate the metabolism of cell, strengthen Skin Cell
Vigor, at the same add with whitening, skin-active, the prepared rehmannia root extract for enhancing metabolism, strengthening Skin Cell vigor and other effects,
Ginseng extract, lemon extract, acted synergistically with five-leaved chaste tree stem cell, play the synergy synergy of whitening spot-removing,
Under plurality of Chinese extract synergy, product can be obviously improved skin quality, improve skin elasticity, improve the hyperplasia energy of skin
Power, positive effect is higher than individually addition plant stem cell.
Embodiment
To better illustrate the object, technical solutions and advantages of the present invention, below in conjunction with specific embodiment to the present invention
It is described further.
Raw material of the present invention is commercially available or prepared using prior art.
A kind of whitening cosmetics containing five-leaved chaste tree stem cell, mainly formed by following weight percent composition, five-leaved chaste tree
Stem cell 5~30%, Ginseng extract 2~15%, prepared rhizome of rehmannia extract solution 2%~15%, lemon extract 2~15%, thickening
Agent 0~0.25%, NMF 15~20%, emulsifying agent 4~8%, preservative 0~0.5%, grease 1.5%~3.5%, three second
Hydramine 0.3-0.6%, surplus form for deionized water.
Thickener in above-mentioned is from the one or more combination referred in card ripple 980, xanthan gum, esters of acrylic acid.
Above-mentioned NMF is from one or more of groups in micromolecule hyaluronic acid sodium, trehalose, glucan, tremella polysaccharides
Close.
Above-mentioned emulsifying agent from lauryl sodium sulfate, AEO, one kind in oliver1000 or
Several combinations.
Foregoing preservatives are from one kind in Phenoxyethanol, potassium sorbate.
A kind of preparation method of the whitening cosmetics containing five-leaved chaste tree stem cell, comprises the following steps：
1) preparation of five-leaved chaste tree stem cell：A) five-leaved chaste tree of the selection without fertilizer and pesticide pollution, the organic plantation of green, and chosen by standard
The newborn branch of ten 10cm length is selected, with aseptic water washing five-leaved chaste tree branch surface attachments 15~20 minutes, newborn branch is put
Enter in 110mg/L ascorbic acid and soak 1min, then surface sterilization is carried out 30 seconds with 75% alcohol, then, with 0.1% liter
Mercury solution sterilizes 10~20 minutes, is finally rinsed repeatedly with sterilized water 5 times, washes away the mercuric chloride raffinate of plant surface；B) after sterilizing
Five-leaved chaste tree branch peel off forming layer, bast, cortex and epidermal tissue with vaccinating lancet, xylem and marrow are abandoned, by the group of acquisition
Knit and be seeded in fresh inducing culture, inoculation 10cm is organized with every 1g2The density of inducing culture be inoculated with, by
Continuously cultivated 10 days for 25 DEG C in the darkroom of control, obtain Cambium tissue；C) Cambium tissue of regeneration and its hetero-organization are separated,
Inoculation solid medium 10cm is organized with every 1g2Inducing culture density, the formation tissue of regeneration is seeded to fresh
In inducing culture, continuously cultivated 10 days for 25 DEG C in controlled darkroom, obtain callus；D) in 1000ml triangular flask
200ml proliferated culture mediums, the 20g of sterilization treatment, diameter 0.5cm bead are added, 10g callus is moved into triangular flask
In, triangular flask is placed in 25 DEG C, cultivated on 120r/min shaking table, is irradiated per hour with 20w incandescent lamp
10min, the 7th day, 1 × 105/ml of the fresh 400ml fluid nutrient mediums to cell density for wherein cell density is added, so
After continue culture 7 days, filtered after culture with the stainless steel mesh of 100 mesh, removing cell mass, residue etc., obtain five-leaved chaste tree cell and hang
Liquid；E) five-leaved chaste tree cell suspending liquid is moved to and large-scale culture is carried out in 20L bioreactors, bioreactor accommodates 5L propagation
Culture medium, the 7th day proliferated culture medium for adding 10L is cultivated, cell suspending liquid is obtained after continuing culture.
Inducing culture：NAA0.5mg/L+BA 2.0mg/L and caseinhydrolysate 1mg/L+MS solid mediums, pH value are
6.0；Proliferated culture medium：2,4-D 2.5mg/L+BA1.5mg/L+ caseinhydrolysates 1mg/L and activated carbon 1mg/L+MS liquid are trained
Support base；PH value is 6.0.
2) preparation of plant extraction liquid：The weight of Chinese medicine material medicine is weighed into ginseng, prepared rhizome of rehmannia, lemon, machinery
Crush as the particle of 0.2~0.5cm particle diameters, add 60% ethanol of 10 times of weight, soak 10 hours under normal temperature, soaked twice.
Active carbon powder is added in extract, stirring is put into 5 degrees Celsius of environment after 5~10 minutes and stands 10~20 hours, supernatant
Liquid crosses 120 mesh sieves, and filtrate is concentrated under reduced pressure under 35 degrees Celsius, is condensed into the equiponderant Chinese medicine with initiation material medicine
Bulk drug alcohol extracting thing concentrate, it is standby.Remaining raw material is commercially available acquisition.
3) in the state of stirring, thickener, emulsifying agent are slowly added into deionized water, heating stirring
To 80-90 DEG C, it is incubated to complete swelling.
4) to NMF is added in 3), stirring is continued to after dissolving completely, 2~4min of homogeneous.
5) grease is added to the mixture in 4), after 3-5min, insulation 10-15min is complete to defoaming, and treats that temperature is down to 55
At~60 DEG C or so, add appropriate triethanolamine and be neutralized to pH to 6.0~7.0 or so, stir.
5) 40~45 DEG C are down to the mass temperature of 6) middle gained, add five-leaved chaste tree stem cell, Ginseng extract, prepared rhizome of rehmannia and carry
Liquid, lemon extract, preservative are taken, is stirred, is cooled to 36 DEG C, stops stirring, discharging.
Embodiment 1
A kind of whitening cosmetics containing five-leaved chaste tree stem cell, mainly formed by following weight percent composition, five-leaved chaste tree
Stem cell 5%, Ginseng extract 2%, prepared rhizome of rehmannia extract solution 4%, lemon extract 2%, card ripple 9800.25%, small molecule are saturating
Bright matter acid sodium 20%, lauryl sodium sulfate 8%, potassium sorbate 0.5%, grease 3.5%, triethanolamine 0.6%, surplus are to go
Ionized water forms.
Preparation method comprises the following steps in embodiment 1：1) preparation of five-leaved chaste tree stem cell：A) selection is dirty without fertilizer and pesticide
Dye, the five-leaved chaste tree of the organic plantation of green, and by the newborn branch of ten 10cm length of criterion picks, with aseptic water washing five-leaved chaste tree branch table
Face attachment 15~20 minutes, newborn branch is put into 1min is soaked in 110mg/L L-AA, then with 75% alcohol
Carry out surface sterilization 30 seconds, then with 0.1% mercuric chloride liquid disinfectant 10~20 minutes, finally rinsed 5 times, washed repeatedly with sterilized water
Remove the mercuric chloride raffinate of plant surface；B) the five-leaved chaste tree branch after sterilizing is peeled off into forming layer, bast, cortex and table with vaccinating lancet
Skin tissue, xylem and marrow are abandoned, the tissue of acquisition is seeded in fresh inducing culture, organize to be inoculated with every 1g
The density of 10cm2 inducing culture is inoculated with, and is continuously cultivated 10 days for 25 DEG C in controlled darkroom, is obtained forming layer group
Knit；C) Cambium tissue of regeneration and its hetero-organization are separated, inoculation solid medium 10cm2 Fiber differentiation is organized with every 1g
The density of base, the formation tissue of regeneration is seeded in fresh inducing culture, 25 DEG C of continuous cultures in controlled darkroom
10 days, obtain callus；D) 200ml proliferated culture mediums, the 20g of sterilization treatment, diameter are added in 1000ml triangular flask
0.5cm bead, 10g callus is moved in triangular flask, triangular flask is placed in 25 DEG C, carried out on 120r/min shaking table
Culture, is irradiated 10min with 20w incandescent lamp per hour, the 7th day, adds fresh 400ml fluid nutrient mediums to cell
Density is 1 × 105/ml of wherein cell density, then proceedes to culture 7 days, is filtered after culture with the stainless steel mesh of 100 mesh,
Cell mass, residue etc. are removed, obtains five-leaved chaste tree cell suspension；E) five-leaved chaste tree cell suspending liquid is moved in 20L bioreactors and carried out
Large-scale culture, bioreactor accommodate 5L proliferated culture medium, cultivate the 7th day proliferated culture medium for adding 10L, continue to train
Cell suspending liquid is obtained after supporting.
Inducing culture：NAA0.5mg/L+BA 2.0mg/L and caseinhydrolysate 1mg/L+MS solid mediums, pH value are
6.0；Proliferated culture medium：2,4-D 2.5mg/L+BA1.5mg/L+ caseinhydrolysates 1mg/L and activated carbon 1mg/L+MS liquid are trained
Support base；PH value is 6.0.
2) preparation of plant extraction liquid：The weight of Chinese medicine material medicine is weighed into ginseng, prepared rhizome of rehmannia, lemon, machinery
Crush as the particle of 0.2~0.5cm particle diameters, add 60% ethanol of 10 times of weight, soak 10 hours under normal temperature, soaked twice.
Active carbon powder is added in extract, stirring is put into 5 degrees Celsius of environment after 5~10 minutes and stands 10~20 hours, supernatant
Liquid crosses 120 mesh sieves, and filtrate is concentrated under reduced pressure under 35 degrees Celsius, is condensed into the equiponderant Chinese medicine with initiation material medicine
Bulk drug alcohol extracting thing concentrate, it is standby.Remaining raw material is commercially available acquisition.
3) in the state of stirring, card ripple 980, lauryl sodium sulfate are slowly added into deionized water, heating is stirred
Mix to 80-90 DEG C, be incubated to complete swelling.
4) added into 3) micromolecule hyaluronic acid sodium, continue stirring to after dissolving completely, 2~4min of homogeneous.
5) grease is added to the mixture in 4), after 3-5min, insulation 10-15min is complete to defoaming, and treats that temperature is down to 55
At~60 DEG C or so, add appropriate triethanolamine and be neutralized to pH to 6.0~7.0 or so, stir.
5) 40~45 DEG C are down to the mass temperature of 4) middle gained, add five-leaved chaste tree stem cell, Ginseng extract, prepared rhizome of rehmannia and carry
Liquid, lemon extract, potassium sorbate are taken, is stirred, is cooled to 36 DEG C, stops stirring, discharging.
Embodiment 2
A kind of whitening cosmetics containing five-leaved chaste tree stem cell, mainly formed by following weight percent composition, five-leaved chaste tree
Stem cell 10%, Ginseng extract 5%, prepared rhizome of rehmannia extract solution 6%, lemon extract 6%, xanthan gum 0.1%, trehalose
18%th, AEO 6%, Phenoxyethanol 0.3%, grease 2%, triethanolamine 0.4%, surplus are deionized water group
Into.
Preparation method step is same as Example 1 in embodiment 2, is not described herein.
Embodiment 3
A kind of whitening cosmetics containing five-leaved chaste tree stem cell, mainly formed by following weight percent composition, five-leaved chaste tree
Stem cell 20%, Ginseng extract 15%, prepared rhizome of rehmannia extract solution 10%, lemon extract 10%, esters of acrylic acid 0.1%, Portugal
Glycan 20%, oliver10007%, potassium sorbate 0.3%, grease 2.5%, triethanolamine 0.5%, surplus are deionized water group
Into.
Preparation method step is same as Example 1 in embodiment 3, is not described herein.
Embodiment 4
A kind of whitening cosmetics containing five-leaved chaste tree stem cell, mainly formed by following weight percent composition, five-leaved chaste tree
Stem cell 30%, Ginseng extract 13%, prepared rhizome of rehmannia extract solution 15%, lemon extract 8%, card ripple 980 and xanthan gum are total
0.25%th, micromolecule hyaluronic acid sodium and trehalose summation 18%, lauryl sodium sulfate 4%, Phenoxyethanol 0.4%, grease
1.5%, triethanolamine 0.3%, surplus forms for deionized water.
Preparation method step is same as Example 1 in embodiment 4, is not described herein.
Embodiment 5
A kind of whitening cosmetics containing five-leaved chaste tree stem cell, mainly formed by following weight percent composition, five-leaved chaste tree
Stem cell 25%, Ginseng extract 8%, prepared rhizome of rehmannia extract solution 2%, lemon extract 15%, card ripple 980 and esters of acrylic acid are total
Totally 0.1%, tremella polysaccharides 15%, AEO 5%, potassium sorbate 0.2%, grease 2%, triethanolamine 0.4%,
Surplus forms for deionized water.
Preparation method step is same as Example 1 in embodiment 5, is not described herein.
The whitening spot-removing clinical trial of the present invention
1) safety detection.Sample prepared by embodiment 1 to 5 is sent to third party testing agency detection skin thorn respectively
Swash property, testing agency to each sample with the continuous skin irritation test of six chicken embryos, the product of each embodiment, its experimental group
Bleeding, blood coagulation and the blood vessel of chicken embryo melt the time and are above 300 minutes, and the stimulation scoring being calculated is zero, illustrates this reality
The sample for applying example 1~5 is nonirritant.
2) hygienic practice detects.Sample prepared by embodiment 1 to 5 is sent into third party testing agency respectively to detect whether
Meet corresponding hygienic practice, third party testing agency by outward appearance, smell, pH value, methanol content, arsenic content, lead content,
The indexs such as mercury content, total plate count, mould, saccharomycete, staphylococcus aureus are detected, and testing result meets regulation will
Ask, finally judge that meeting embodiment 1~5 meets《Cosmetics health specification》Limitation requirement.
Control group 1
A kind of whitening cosmetics, mainly formed by following weight percent composition, five-leaved chaste tree stem cell 30%, card ripple
980 total with xanthan gum 0.25%, micromolecule hyaluronic acid sodium and trehalose summation 18%, lauryl sodium sulfate 4%, benzene oxygen second
Alcohol 0.4%, grease 1.5%, triethanolamine 0.3%, surplus form for deionized water.
Its preparation method is same as Example 4, is not described herein.
Control group 2
A kind of whitening cosmetics, mainly formed by following weight percent composition, Ginseng extract 13%, card ripple
980 total with xanthan gum 0.25%, micromolecule hyaluronic acid sodium and trehalose summation 18%, lauryl sodium sulfate 4%, benzene oxygen second
Alcohol 0.4%, grease 1.5%, triethanolamine 0.3%, surplus form for deionized water.Its preparation method is same as Example 4,
This is not described.
Control group 3
A kind of whitening cosmetics, mainly formed by following weight percent composition, prepared rhizome of rehmannia extract solution 15%, card ripple
980 total with xanthan gum 0.25%, micromolecule hyaluronic acid sodium and trehalose summation 18%, lauryl sodium sulfate 4%, benzene oxygen second
Alcohol 0.4%, grease 1.5%, triethanolamine 0.3%, surplus form for deionized water.
Control group 4
A kind of whitening cosmetics, mainly formed by following weight percent composition, lemon extract 8%, card ripple 980
Total with xanthan gum 0.25%, micromolecule hyaluronic acid sodium and trehalose summation 18%, lauryl sodium sulfate 4%, Phenoxyethanol
0.4%, grease 1.5%, triethanolamine 0.3%, surplus is deionized water composition.Its preparation method is same as Example 4, herein
It is not described.
Control group 5
A kind of whitening cosmetics, mainly formed by following weight percent composition, five-leaved chaste tree stem cell 30%, ginseng carry
Take liquid 13%, card ripple 980 and xanthan gum total 0.25%, micromolecule hyaluronic acid sodium and trehalose summation 18%, dodecyl sulphur
Sour sodium 4%, Phenoxyethanol 0.4%, grease 1.5%, triethanolamine 0.3%, surplus form for deionized water.Its preparation method with
Embodiment 4 is identical, is not described herein.
Control group 6
A kind of whitening cosmetics, mainly formed by following weight percent composition, five-leaved chaste tree stem cell 30%, prepared rhizome of rehmannia
Extract solution 15%, card ripple 980 and xanthan gum are total 0.25%, micromolecule hyaluronic acid sodium and trehalose summation 18%, dodecyl
Sodium sulphate 4%, Phenoxyethanol 0.4%, grease 1.5%, triethanolamine 0.3%, surplus form for deionized water.Its preparation method
It is same as Example 4, it is not described herein.
Control group 7
A kind of whitening cosmetics, mainly formed by following weight percent composition, five-leaved chaste tree stem cell 30%, lemon carry
Take liquid 8%, card ripple 980 and xanthan gum total 0.25%, micromolecule hyaluronic acid sodium and trehalose summation 18%, dodecyl sulphate
Sodium 4%, Phenoxyethanol 0.4%, grease 1.5%, triethanolamine 0.3%, surplus form for deionized water.Its preparation method and reality
It is identical to apply example 4, is not described herein.
Control group 8
A kind of whitening cosmetics, mainly formed by following weight percent composition, Ginseng extract 13%, prepared rhizome of rehmannia
Extract solution 15%, card ripple 980 and xanthan gum are total 0.25%, micromolecule hyaluronic acid sodium and trehalose summation 18%, dodecyl
Sodium sulphate 4%, Phenoxyethanol 0.4%, grease 1.5%, triethanolamine 0.3%, surplus form for deionized water.Its preparation method
It is same as Example 4, it is not described herein.
Control group 9
A kind of whitening cosmetics, mainly formed by following weight percent composition, Ginseng extract 13%, lemon carry
Take liquid 8%, card ripple 980 and xanthan gum total 0.25%, micromolecule hyaluronic acid sodium and trehalose summation 18%, dodecyl sulphate
Sodium 4%, Phenoxyethanol 0.4%, grease 1.5%, triethanolamine 0.3%, surplus form for deionized water.Its preparation method and reality
It is identical to apply example 4, is not described herein.
Control group 10
A kind of whitening cosmetics, mainly formed by following weight percent composition, prepared rhizome of rehmannia extract solution 15%, lemon
Extract solution 8%, card ripple 980 and xanthan gum are total 0.25%, micromolecule hyaluronic acid sodium and trehalose summation 18%, dodecyl sulphur
Sour sodium 4%, Phenoxyethanol 0.4%, grease 1.5%, triethanolamine 0.3%, surplus form for deionized water.Its preparation method with
Embodiment 4 is identical, is not described herein.
Control group 11
A kind of whitening cosmetics, mainly formed by following weight percent composition, five-leaved chaste tree stem cell 30%, ginseng carry
Take liquid 13%, prepared rhizome of rehmannia extract solution 15%, card ripple 980 total with xanthan gum 0.25%, micromolecule hyaluronic acid sodium and trehalose total
It is deionized water with 18%, lauryl sodium sulfate 4%, Phenoxyethanol 0.4%, grease 1.5%, triethanolamine 0.3%, surplus
Composition.Its preparation method is same as Example 4, is not described herein.
Control group 12
A kind of whitening cosmetics, mainly formed by following weight percent composition, five-leaved chaste tree stem cell 30%, prepared rhizome of rehmannia
Extract solution 15%, lemon extract 8%, card ripple 980 and xanthan gum are total 0.25%, micromolecule hyaluronic acid sodium and trehalose summation
18%th, lauryl sodium sulfate 4%, Phenoxyethanol 0.4%, grease 1.5%, triethanolamine 0.3%, surplus are deionized water group
Into.Its preparation method is same as Example 4, is not described herein.
Control group 13
A kind of whitening cosmetics, mainly formed by following weight percent composition, Ginseng extract 13%, prepared rhizome of rehmannia
Extract solution 15%, lemon extract 8%, card ripple 980 and xanthan gum are total 0.25%, micromolecule hyaluronic acid sodium and trehalose summation
18%th, lauryl sodium sulfate 4%, Phenoxyethanol 0.4%, grease 1.5%, triethanolamine 0.3%, surplus are deionized water group
Into.Its preparation method is same as Example 4, is not described herein.
Control group 14
A kind of whitening cosmetics, mainly formed by following weight percent composition, five-leaved chaste tree stem cell 30%, ginseng carry
Take that liquid 13%, prepared rhizome of rehmannia extract solution 15%, lemon extract 8%, card ripple 980 and xanthan gum is total 0.25%, small molecule hyalomitome
Sour sodium and trehalose summation 18%, lauryl sodium sulfate 4%, Phenoxyethanol 0.4%, grease 1.5%, triethanolamine 0.3%,
Surplus forms for deionized water.Its preparation method is same as Example 4, is not described herein.
Control group 15
A kind of whitening cosmetics, mainly formed by following weight percent composition, five-leaved chaste tree stem cell 35%, ginseng carry
Take liquid 1%, prepared rhizome of rehmannia extract solution 18%, lemon extract 18%, card ripple 980 0.25%, micromolecule hyaluronic acid sodium 20%,
Lauryl sodium sulfate 8%, potassium sorbate 0.5%, grease 3.5%, triethanolamine 0.6%, surplus form for deionized water.Its
Preparation method is same as Example 4, is not described herein.
Control group 16
A kind of whitening cosmetics, mainly formed by following weight percent composition, five-leaved chaste tree stem cell 3%, ginseng carry
Take liquid 18%, prepared rhizome of rehmannia extract solution 1%, lemon extract 1%, card ripple 980 0.25%, micromolecule hyaluronic acid sodium 20%, ten
Sodium dialkyl sulfate 8%, potassium sorbate 0.5%, grease 3.5%, triethanolamine 0.6%, surplus form for deionized water.It is made
Preparation Method is same as Example 4, is not described herein.
Experiment effect
(1) general information
Tested group：The whitening cosmetics of the present invention are all understood 100 observations with regard to 1~5 every group of embodiment of embodiment
Whitening spot-removing effect；
Control group：Whitening spot-removing effect all is understood to 100 observations with regard to 1~7 every group of experimental example of control group.
Experimenter is women, and at 18~60 years old age, Head And Face is visible different degrees of dim heavy, blackspot, chloasma pigment
Symptom is precipitated, without symptoms such as any sufferings.
Control group：
(2) application method：Tested group is that avoiding eye is applied to face sooner or later with control group, once in the morning and once at night.
(3) result judgment criteria：
Effectively：Experimenter feels very satisfied, and facial colour spot substantially subtracts light or complete regression, skin elasticity increase,
Skin shine increase, skin substantially bleach.
It is effective：Experimenter feels general satisfaction, and facial colour spot slightly subtracts light, skin elasticity increase, skin shine increasing
Add, skin is slightly aobvious to bleach.
It is invalid：Experimenter feels dissatisfied, and facial colour spot is without improvement, skin elasticity increase, skin skin shine, bullet
Property and white temporarily Cheng Du be not any change.
(4) result of the test
Table 1
It was found from the embodiment in table 1, more than 83% subject is significantly effective, and facial colour spot substantially subtracts light or disappeared completely
Move back, skin elasticity increase, skin shine increase, skin substantially bleaches, wherein more excellent with embodiment 3,4 effects.
Control group 1~4 is respectively only using five-leaved chaste tree stem cell, Ginseng extract, prepared rhizome of rehmannia extract solution, lemon extract system
Standby whitening cosmetics, above-mentioned 1~4 condition same as Example 4 of experimental example, it can be seen that be used alone in four kinds
A kind of extract, its DeGrain, relative to the application, its whitening spot-removing is clearly worse, effective composition below 48%,
And have it is more than 25% invalid.
Control group 5~10 is the skin care item obtained using two kinds in four kinds of extracts any combination, with embodiment 4 other
Condition is identical, it can be seen that relative to extract is used alone, its whitening spot-removing effect has lifting, but its effect is gone back with the present invention
Have differences, experimental example 11~14 is the skin care item obtained using any three kinds of combinations in four kinds of extracts, itself and embodiment 4
Other conditions are identical, where it can be seen that relative to combination of two, effect has a certain upgrade, but distinguish it is little, and with the present invention
Compare, effect has differences, and under illustrating that the composition need of the present invention act synergistically jointly, can just reach significant whitening and dispel
Spot effect.
Control group 15~16 is that its formula rate is at this under the conditions of use identical composition, identical preparation method
Outside the specific proportion of invention, its effect is nothing like the effect of the present invention, it was demonstrated that, composition of the invention only exists
Competence exertion is significantly dispelled pouch effect in special ratios.
(5) notable case
Case 1：Ms Zhang：35 years old, colleges and universities computer control personnel were engaged in computer specialty 7 years, and the colour of skin is obscure, suffers from chloasma
(be thought of as computer radiation caused by) was not once imitated with many skin-lightening cosmetics within more than 1 year, using after the cosmetics of the present invention after 3 months,
The colour of skin fine and smooth, chloasma that bleaches disappears.
Case 2：Mrs Liu：26 years old, certain Supermarket management person, the year before after the boy of pregnancy and childbearing one, there is cyasma in face
Do not disappear always, after using cosmetics of the present invention 2 months, cyasma is disappeared, and follow-up is not recurred so far.
Case 3：Mrs Ou Yang, 15 years old, student, suffer from freckle from small, once dare not with skin is injured after the medicine for freckle of dispelling
Medication, see that its facial freckle gathers whole face, and see using the scar left after its medicine altogether, it is proposed that cosmetics of the present invention are used,
It is equipped with egg white and adjusts and know, application disappears substantially after June.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
God any modification, equivalent substitution and improvements done etc., should be included within the scope of protection of the invention with principle.
Claims (6)
1. a kind of whitening cosmetics, the component containing following weight/mass percentage composition, five-leaved chaste tree stem cell 5~30%, ginseng carry
Take liquid 2~15%, prepared rhizome of rehmannia extract solution 2%~15%, lemon extract 2~15%.
A kind of 2. whitening cosmetics according to claim 1, it is characterised in that：Also include thickener 0~0.25%,
NMF 15~20%, emulsifying agent 4~8%, preservative 0~0.5%, grease 1.5%~3.5%, triethanolamine 0.3-
0.6%, surplus forms for deionized water；
Described thickener is from the one or more combination referred in card ripple 980, xanthan gum, esters of acrylic acid；
The NMF is from one or more combination in micromolecule hyaluronic acid sodium, trehalose, glucan, tremella polysaccharides；
Described emulsifying agent is from the one or more in lauryl sodium sulfate, AEO, oliver1000
Combination；
The preservative is from one kind in Phenoxyethanol, potassium sorbate.
A kind of 3. whitening cosmetics according to claim 1, it is characterised in that：The five-leaved chaste tree stem cell be 10~
30%th, the Ginseng extract is 8-15%, the prepared rhizome of rehmannia extract solution is 6%~15%, the lemon extract be 6~
10%.
A kind of 4. whitening cosmetics according to claim 1, it is characterised in that：The five-leaved chaste tree stem cell be 20%,
Ginseng extract is 15%, prepared rhizome of rehmannia extract solution is 10%, lemon extract 10%.
A kind of 5. whitening cosmetics according to claim 1, it is characterised in that：The five-leaved chaste tree stem cell be 30%,
Ginseng extract is 13%, prepared rhizome of rehmannia extract solution is 15%, lemon extract 8%.
A kind of 6. preparation method of whitening cosmetics according to any one of claim 1 to 5, it is characterised in that：Bag
Include following steps：
1) preparation of five-leaved chaste tree stem cell：A) after the new branch sterilization of five-leaved chaste tree, xylem and marrow being removed, inducing culture is inoculated in,
Inducing culture obtains cambial cell；B) cambial cell obtains through culture medium through subculture, access proliferated culture medium, shaking table culture
Obtain unicellular；C) the unicellular proliferated culture medium that is inoculated in is cultivated through expanding, and obtains five-leaved chaste tree stem cell；
2) preparation of plant extraction liquid：The weight of raw material is weighed into ginseng, prepared rhizome of rehmannia, lemon, mechanical crushing is 0.2~
The particle of 0.5cm particle diameters, 60% ethanol of 10 times of weight is added, is soaked 10 hours under normal temperature, immersion is twice.Add in extract
Entering active carbon powder, stirring is put into 5 degrees Celsius of environment after 5~10 minutes and stands 10~20 hours, and supernatant crosses 120 mesh sieves,
Filtrate is concentrated under reduced pressure under 35 degrees Celsius, is condensed into the equiponderant Chinese medicine material medicine alcohol extracting with initiation material medicine
Thing concentrate, it is standby；
3) in the state of stirring, thickener, emulsifying agent are slowly added into deionized water, heating stirring is protected to 80-90 DEG C
Temperature is to complete swelling；
4) to NMF is added in 3), stirring is continued to after dissolving completely, 2~4min of homogeneous；
5) grease is added to the mixture in 4), after 3-5min, insulation 10-15min is complete to defoaming, and treats that temperature is down to 55~60
DEG C or so when, add appropriate triethanolamine and be neutralized to pH to 6.0~7.0 or so, stir；
6) 40~45 DEG C are down to the mass temperature of 5) middle gained, add five-leaved chaste tree stem cell, Ginseng extract, prepared rhizome of rehmannia extraction
Liquid, lemon extract, preservative, stir, and are cooled to 36 DEG C, stop stirring, discharging；
The inducing culture is the MS solid mediums containing NAA, BA and caseinhydrolysate, the proliferated culture medium be containing
2,4-D, BA, caseinhydrolysate and activated carbon MS fluid nutrient mediums.
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Cited By (2)
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| CN111973548A (en) * | 2020-09-10 | 2020-11-24 | 广东丸美生物技术股份有限公司 | Eye essence containing negundo chastetree stem cell extract and preparation method thereof |
| CN112852707A (en) * | 2019-11-27 | 2021-05-28 | 深圳欧珈再生医学抗衰生物工程有限公司 | Bud stem cell, extraction method and bud stem cell essence |
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| CN105748343A (en) * | 2016-02-25 | 2016-07-13 | 广州市科玮生物技术有限公司 | Natural plant extract composition and application thereof in cosmetics |
| CN106860088A (en) * | 2017-02-09 | 2017-06-20 | 曼秀雷敦(中国)药业有限公司 | A kind of whitening is dispelled scar skin care item and preparation method thereof |
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| CN104920227A (en) * | 2015-07-15 | 2015-09-23 | 广东融和生态农业有限公司 | Stem cell tissue culture seedling raising technique |
| CN105342867A (en) * | 2015-12-15 | 2016-02-24 | 上海相宜本草化妆品股份有限公司 | Herbal composition submicron lipid carrier and preparation method thereof |
| CN105748343A (en) * | 2016-02-25 | 2016-07-13 | 广州市科玮生物技术有限公司 | Natural plant extract composition and application thereof in cosmetics |
| CN106860088A (en) * | 2017-02-09 | 2017-06-20 | 曼秀雷敦(中国)药业有限公司 | A kind of whitening is dispelled scar skin care item and preparation method thereof |
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| CN112852707A (en) * | 2019-11-27 | 2021-05-28 | 深圳欧珈再生医学抗衰生物工程有限公司 | Bud stem cell, extraction method and bud stem cell essence |
| CN111973548A (en) * | 2020-09-10 | 2020-11-24 | 广东丸美生物技术股份有限公司 | Eye essence containing negundo chastetree stem cell extract and preparation method thereof |
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Application publication date: 20171208 |