CN107435059A - Prepare method, prepared dried orange peel extracts and its application of dried orange peel extracts - Google Patents
Prepare method, prepared dried orange peel extracts and its application of dried orange peel extracts Download PDFInfo
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- CN107435059A CN107435059A CN201610370773.8A CN201610370773A CN107435059A CN 107435059 A CN107435059 A CN 107435059A CN 201610370773 A CN201610370773 A CN 201610370773A CN 107435059 A CN107435059 A CN 107435059A
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- orange peel
- dried orange
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- cellulase
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- 238000000034 method Methods 0.000 title claims abstract description 30
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 48
- 238000006243 chemical reaction Methods 0.000 claims abstract description 25
- 108010059892 Cellulase Proteins 0.000 claims abstract description 17
- 229940106157 cellulase Drugs 0.000 claims abstract description 17
- 238000001976 enzyme digestion Methods 0.000 claims abstract description 16
- 239000002994 raw material Substances 0.000 claims abstract description 4
- QUQPHWDTPGMPEX-UTWYECKDSA-N aurantiamarin Natural products COc1ccc(cc1O)[C@H]1CC(=O)c2c(O)cc(O[C@@H]3O[C@H](CO[C@@H]4O[C@@H](C)[C@H](O)[C@@H](O)[C@H]4O)[C@@H](O)[C@H](O)[C@H]3O)cc2O1 QUQPHWDTPGMPEX-UTWYECKDSA-N 0.000 claims description 42
- 108090000790 Enzymes Proteins 0.000 claims description 22
- 102000004190 Enzymes Human genes 0.000 claims description 22
- 229940088598 enzyme Drugs 0.000 claims description 22
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 15
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 10
- 238000010792 warming Methods 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 230000033228 biological regulation Effects 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 230000035484 reaction time Effects 0.000 claims description 6
- 235000013305 food Nutrition 0.000 claims description 5
- 239000001814 pectin Substances 0.000 claims description 5
- 229920001277 pectin Polymers 0.000 claims description 5
- 235000010987 pectin Nutrition 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 9
- 238000000638 solvent extraction Methods 0.000 abstract description 3
- 239000002904 solvent Substances 0.000 abstract description 2
- 238000000605 extraction Methods 0.000 description 40
- 230000000694 effects Effects 0.000 description 9
- 238000003756 stirring Methods 0.000 description 7
- 241001672694 Citrus reticulata Species 0.000 description 6
- 239000001913 cellulose Substances 0.000 description 6
- 229920002678 cellulose Polymers 0.000 description 6
- 239000012141 concentrate Substances 0.000 description 5
- 238000004128 high performance liquid chromatography Methods 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 239000004480 active ingredient Substances 0.000 description 3
- 230000003197 catalytic effect Effects 0.000 description 3
- 210000002421 cell wall Anatomy 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000002253 acid Substances 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- -1 flavanone glucoside compound Chemical class 0.000 description 2
- 229930182470 glycoside Natural products 0.000 description 2
- 150000002338 glycosides Chemical class 0.000 description 2
- 230000037306 mature skin Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 239000001100 (2S)-5,7-dihydroxy-2-(3-hydroxy-4-methoxyphenyl)chroman-4-one Substances 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- QUQPHWDTPGMPEX-UHFFFAOYSA-N Hesperidine Natural products C1=C(O)C(OC)=CC=C1C1OC2=CC(OC3C(C(O)C(O)C(COC4C(C(O)C(O)C(C)O4)O)O3)O)=CC(O)=C2C(=O)C1 QUQPHWDTPGMPEX-UHFFFAOYSA-N 0.000 description 1
- 206010054949 Metaplasia Diseases 0.000 description 1
- 241001093501 Rutaceae Species 0.000 description 1
- ZONYXWQDUYMKFB-UHFFFAOYSA-N SJ000286395 Natural products O1C2=CC=CC=C2C(=O)CC1C1=CC=CC=C1 ZONYXWQDUYMKFB-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
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- 230000001476 alcoholic effect Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 210000000748 cardiovascular system Anatomy 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- APSNPMVGBGZYAJ-GLOOOPAXSA-N clematine Natural products COc1cc(ccc1O)[C@@H]2CC(=O)c3c(O)cc(O[C@@H]4O[C@H](CO[C@H]5O[C@@H](C)[C@H](O)[C@@H](O)[C@H]5O)[C@@H](O)[C@H](O)[C@H]4O)cc3O2 APSNPMVGBGZYAJ-GLOOOPAXSA-N 0.000 description 1
- 230000009849 deactivation Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
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- 229940079593 drug Drugs 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 229930003949 flavanone Natural products 0.000 description 1
- 235000011981 flavanones Nutrition 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 229930182478 glucoside Natural products 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 235000008216 herbs Nutrition 0.000 description 1
- QUQPHWDTPGMPEX-QJBIFVCTSA-N hesperidin Chemical compound C1=C(O)C(OC)=CC=C1[C@H]1OC2=CC(O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@@H](CO[C@H]4[C@@H]([C@H](O)[C@@H](O)[C@H](C)O4)O)O3)O)=CC(O)=C2C(=O)C1 QUQPHWDTPGMPEX-QJBIFVCTSA-N 0.000 description 1
- VUYDGVRIQRPHFX-UHFFFAOYSA-N hesperidin Natural products COc1cc(ccc1O)C2CC(=O)c3c(O)cc(OC4OC(COC5OC(O)C(O)C(O)C5O)C(O)C(O)C4O)cc3O2 VUYDGVRIQRPHFX-UHFFFAOYSA-N 0.000 description 1
- 229940025878 hesperidin Drugs 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000015689 metaplastic ossification Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000019462 natural additive Nutrition 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- ARGKVCXINMKCAZ-UHFFFAOYSA-N neohesperidine Natural products C1=C(O)C(OC)=CC=C1C1OC2=CC(OC3C(C(O)C(O)C(CO)O3)OC3C(C(O)C(O)C(C)O3)O)=CC(O)=C2C(=O)C1 ARGKVCXINMKCAZ-UHFFFAOYSA-N 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000002195 soluble material Substances 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 238000001291 vacuum drying Methods 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
- C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
- A61K36/752—Citrus, e.g. lime, orange or lemon
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Alternative & Traditional Medicine (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a kind of method for preparing dried orange peel extracts, including using dried orange peel as raw material, dried orange peel extracts are prepared using cellulase and pectase joint enzymolysis.Invention additionally discloses the application of the dried orange peel extracts and the extract prepared using the method.The present invention has the characteristics that efficiency high, easy to operate, reaction condition is gentle using the method for cellulase and pectase joint enzymolysis.Using ethanol as solvent extraction after enzyme digestion reaction, this method yield is high, safe and effective, and solvent is recyclable, suitable for industrialized production.
Description
Technical field
The invention belongs to Separation of Natural Products technical field, is related to a kind of method for preparing dried orange peel extracts, dried orange peel extraction
Thing and its application.
Background technology
Dried orange peel is rutaceae orange and its dry mature skin of variety, and dried orange peel medicinal material is divided into " dried orange peel " and " wide
Dried orange peel "." citrus chachiensis hortorum " is the dry mature skin of citrus reticulata"Chachi”, and main product is one of ten big authentic medicinal herbs of Guangdong in Xinhui of Guangdong Province.Extensively
The active ingredient of dried orange peel has volatile oil and Flavonoid substances (wherein based on aurantiamarin), the typically content by determining aurantiamarin
Quality index as evaluation dried orange peel.Aurantiamarin category flavanone glucoside compound, is one of main component of dried orange peel.Research
It was found that aurantiamarin has a variety of medicines such as anti-inflammatory, anti-oxidant, antibacterial, anticancer, regulation immunity, radiation proof, protection cardiovascular system
Reason activity, and natural active matter is used as, it has no toxic side effect, and may be used as the natural additive of food, medicine etc., is eating
Product, medicine and daily-use chemical industry industry have a wide range of applications.
At present, the extracting method of aurantiamarin has water extraction and organic solvent extraction in dried orange peel.It is however, effective in dried orange peel
Composition is almost surrounded by plant cell wall, and traditional extracting method can not make the cell wall rupture of encirclement aurantiamarin, exists huge
Big resistance to mass tranfer, causes extraction effect to be very restricted, and recovery rate is relatively low, and extraction effect is undesirable, causes resource unrestrained
Take serious.
The content of the invention
In view of above-mentioned the deficiencies in the prior art, high it is an object of the invention to provide a kind of extraction efficiency, reaction condition temperature
The method for preparing dried orange peel extracts of sum.
The present invention provides a kind of method for preparing dried orange peel extracts, including using dried orange peel as raw material, using cellulase and fruit
The enzyme-linked synthase solution of glue prepares dried orange peel extracts.
Another aspect of the present invention provides the dried orange peel extracts prepared using the above method.
In one embodiment of the invention, the yield of aurantiamarin is 4.28-4.4% in the dried orange peel extracts.
The present invention also provides application of the above-mentioned dried orange peel extracts in liquid or solid health-care food is prepared.
In dried orange peel extracts prepared by the present invention, content of hesperidin is high.Digested using cellulase and pectase joint
Method, the connected pectin of the cell wall structure and its iuntercellular that can be sufficiently destroyed based on cellulose, reduce the mass transfer resistance of extraction
Power, so as to improve the extract yield of active ingredient.And enzyme has high catalytic efficiency, and selectivity is strong, reaction condition gently waits spy
Point, is extracted using cellulase and pectase, and production equipment is simple, and small investment, reaction product is safe and non-toxic, suitable for industry
Metaplasia is produced.
Embodiment
The present invention provides a kind of preparation method of dried orange peel extracts, the dried orange peel extracts prepared and application, to make the present invention
Purpose, technical scheme and effect it is clearer, clear and definite, the present invention is described in more detail below.It should be appreciated that this place
The specific embodiment of description only to explain the present invention, is not intended to limit the present invention.
Aurantiamarin is the main active substances in dried orange peel, and the present invention is using the content of aurantiamarin in measurement dried orange peel as evaluation
The quality standard of dried orange peel extraction efficiency and dried orange peel extracts.
One aspect of the present invention provides a kind of method for preparing dried orange peel extracts, including using dried orange peel as raw material, using cellulose
Enzyme and pectase joint enzymolysis prepare dried orange peel extracts.
In one embodiment of the invention, the total amount of the cellulase and pectase accounts for the 2.5- of dried orange peel dosage
4.0%.
In one embodiment of the invention, the proportioning of the cellulase and pectase is 1:2-6.
In one embodiment of the invention, comprise the following steps：
Dried orange peel adds water to be warming up to 50-60 DEG C, and regulation solution ph is 3.0-3.5, then adds the cellulose of special ratios
Enzyme and pectase, enzyme digestion reaction is carried out, the reaction time is 2-3 hours, and the dried orange peel after enzymolysis is made；
After the completion of the enzyme digestion reaction, ethanol solution is added into the dried orange peel after enzymolysis, is warming up to 80-90 DEG C, carries out alcohol
Reaction is proposed, the reaction time is 2-3 hours, is filtered, concentration, dries obtained dried orange peel extracts.
In one embodiment of the invention, the dried orange peel needs to first pass through pulverization process.It should be understood that the crushing
Method can be crushed using those skilled in the art's conventional method, such as pulverizer etc..
In one embodiment of the invention, the proportioning of the dried orange peel and water is 1:8-10.This proportioning is that inventor passes through
Many experiments are obtained, if because the proportioning of dried orange peel and water is too low, cellulase and pectase can not be thoroughly dispersed in water, are influenceed
Contact of the enzyme with dried orange peel, causes the yield of aurantiamarin to reduce.But too high proportioning can dilute the useful effect concentration of enzyme, drop again
The catalytic activity of low enzyme, so as to reduce the yield of aurantiamarin.Content (mg)/dried orange peel of yield=aurantiamarin of the aurantiamarin
Quality (mg) × 100%.
In one embodiment of the invention, the heating is heated up using steam, is warming up to 50-60 DEG C.Enzyme digestion reaction has
Suitable reaction temperature, temperature is too low, and enzyme activity is relatively low, it is impossible to gives full play to effect of enzymolysis.But temperature is too high will
Make enzyme denaturation, enzyme digestion reaction is suppressed, and causes recovery rate to reduce.
In one embodiment of the invention, acid, alkali is used to adjust pH value as 3.0-3.5, the acid, alkali are respectively hydrochloric acid
And/or sodium hydroxide.PH value, which influences the activated centre of enzyme or the dissociated state of group associated therewith, enzyme digestion reaction, to be had suitably
PH value, the catalytic rate highest of enzyme under the conditions of this pH.In pH value 3.0-3.5, in cellulase and pectase most
Good action pH value section, both maximum vigor can be played, destroy cell membrane, reduced resistance to mass tranfer, be advantageous to aurantiamarin
Dissolution.
In one embodiment of the invention, by mass percentage, the amount of the cellulase of the addition is 0.5-
1.0%th, the amount of pectase is 2.0-3.0%.If enzyme concentration is too low, enzymolysis fails to carry out completely.But the timing of dried orange peel amount one,
With the raising of enzyme concentration, dried orange peel concentration can not reach saturation to enzyme, and enzymolysis is suppressed, therefore enzyme concentration is not bigger
Better.
In one embodiment of the invention, the enzyme digestion reaction time is 2-3 hours.With the progress of enzyme digestion reaction,
The barrier layer that the cellulose and pectin of dried orange peel cell membrane and cell interbed are formed effectively is degraded, and aurantiamarin gradually expands from intracellular
Shed and.But with the progress of reaction, the aurantiamarin concentration of intraor extracellular has reached a kind of dynamic equilibrium, when continuing to extend extraction
Between effect to improving aurantiamarin concentration in extract solution it is limited, and the product of the enzyme digestion reaction production concentration of cellulase and pectase
The progress of enzyme digestion reaction will be suppressed by tiring out, and overlong time will extend manufacture cycle in addition, be unfavorable for industrialized production.
In one embodiment of the invention, when ethanol solution is added into the dried orange peel after enzymolysis, dried orange peel and ethanol solution
Proportioning be 1：22-30.Although the proportioning of dried orange peel and ethanol solution is bigger, the stripping quantity of aurantiamarin is bigger in dried orange peel, aurantiamarin
Yield can also improve, but extract solution dosage it is excessive both caused extracts reagent consumption increase, increase production cost, follow-up work can be given again
Skill makes troubles.
The alcohol extracting reaction time is 2-3 hours, is because extraction time extends the dried orange peel and ethanol that can increase after digesting
The time of contact of solution, improve the solubility of active ingredient, the rise of aurantiamarin yield.But after the time continues extension, after enzymolysis
Other alcohol-soluble materials in dried orange peel also can be dissolved out, influence aurantiamarin yield, and overlong time will extend production week
Phase, it is unfavorable for industrialized production.
In one embodiment of the invention, the ethanol solution concentration is 65-70%.With carrying for ethanol solution concentration
Height, the osmotic pressure of the dried orange peel after enzymolysis are improved, and create the condition to external diffusion so that the yield of aurantiamarin gradually carries
It is high；And concentration of alcohol is too high that the stripping quantity of some alcohol dissolubility impurities and liposoluble substance can be made also accordingly to increase, they with ethanol-
Moisture subsystem combines, and influences the yield of aurantiamarin.
In one embodiment of the invention, alcohol extracting reaction temperature is 80-90 DEG C, be because high temperature to improve molecule random
The then severity of warm-up movement, solubility increase of the aurantiamarin in ethanol solution, yield improve.Meanwhile lasting high temperature carries
Enzyme will be inactivated by taking, and reach the purpose of enzyme deactivation, so as to save energy consumption and ensure the security of final products.
In one embodiment of the invention, after the completion of alcohol extracting reaction, in addition to filter, concentrate, dry.
It should be understood that the filtering can use the conventional filters of those skilled in the art, such as filter paper, filter etc..
The present invention is filtered using duplex strainer.
It should be understood that the concentration can use the conventional concentration means of those skilled in the art, for example, ultrafiltration, absorption method or
Distillation under vacuum etc..The present invention uses vacuum-concentrcted.
It should be understood that the drying can use the conventional drying means of those skilled in the art, such as roller drying, freezing to do
Dry, vacuum drying etc..The present invention is using spray drying.
Another aspect of the present invention provides a kind of dried orange peel extracts prepared using the above method.
In one embodiment of the invention, the yield of aurantiamarin is 4.28-4.4% in the dried orange peel extracts.Orange peel
Glycosides is the main active substances in dried orange peel, and the present invention is using the yield of aurantiamarin in measurement dried orange peel as evaluation dried orange peel extraction efficiency
And the quality standard of dried orange peel extracts.The yield of aurantiamarin middle orange peel compared with prior art in the dried orange peel extracts that the present invention protects
The yield of glycosides is high, it was demonstrated that active component content is high in dried orange peel extracts of the invention, and quality is good.
It should be understood that the method that the present invention measures the yield of the aurantiamarin in dried orange peel extracts can use this area conventional
Method is detected, such as ultraviolet spectrophotometry, high performance liquid chromatography etc..
Another aspect of the present invention provides application of the above-mentioned dried orange peel extracts in liquid or solid health-care food is prepared.
In one embodiment of the invention, the dried orange peel extracts are used to prepare infant care food or health drink
In application.
Embodiment one
The present invention provides a kind of specific embodiment for preparing dried orange peel extracts, and step is as follows：
Dried orange peel is crushed, a certain amount of Dried Tangerine Peel is accurately weighed and pours into extraction vessel, by solid-to-liquid ratio 1：10
Ratio adds water；Steam is passed through into the chuck of extraction vessel, maintains 50-60 DEG C of the temperature of extract solution；Add into extraction vessel
Enter the pH value 3.0-3.5 of the hydrochloric acid prepared or sodium hydroxide regulation extract solution；0.5% fibre is separately added into extract solution
Tie up plain enzyme and 2.0% pectase, stirring extraction 3h.
Enzyme digestion reaction adds the ethanol solution measured after terminating so that extraction conditions now are solid-to-liquid ratio 1：30th, second
Determining alcohol 65%, extraction vessel are warming up to extract solution temperature and reach 80 DEG C, continue stirring extraction 3h；Extraction terminates rear extract solution warp
Duplex strainer filters；The concentrated system vacuum of extract solution after filtering is concentrated under reduced pressure into no alcohol taste；Concentrate is spray-dried
Obtain dried orange peel extracts.
Detected through high performance liquid chromatography, the yield of aurantiamarin is 4.4% in obtained dried orange peel extracts.
Embodiment two
Dried orange peel is crushed, a certain amount of Dried Tangerine Peel is accurately weighed and pours into extraction vessel, by solid-to-liquid ratio 1：9 ratio
Example adds water；Steam is passed through into the chuck of extraction vessel, maintains 50-60 DEG C of the temperature of extract solution；Added into extraction vessel
The pH value 3.0-3.5 of hydrochloric acid or sodium hydroxide the regulation extract solution prepared；1% cellulose is separately added into extract solution
Enzyme and 3% pectase, stirring extraction 2h.
Enzyme digestion reaction adds the ethanol solution measured after terminating so that extraction conditions now are solid-to-liquid ratio 1：35th, second
Determining alcohol 70%, extraction vessel are warming up to extract solution temperature and reach 85 DEG C, continue stirring extraction 2h；Extraction terminates rear extract solution warp
Duplex strainer filters；The concentrated system vacuum of extract solution after filtering is concentrated under reduced pressure into no alcohol taste；Concentrate is spray-dried
Obtain aurantiamarin.
Detected through high performance liquid chromatography, the yield of aurantiamarin is 4.28% in obtained dried orange peel extracts.
Embodiment three
Dried orange peel is crushed, a certain amount of Dried Tangerine Peel is accurately weighed and pours into extraction vessel, by solid-to-liquid ratio 1：8 ratio
Example adds water；Steam is passed through into the chuck of extraction vessel, maintains 50-60 DEG C of the temperature of extract solution；Added into extraction vessel
The pH value 3.0-3.5 of hydrochloric acid or sodium hydroxide the regulation extract solution prepared；1% cellulose is separately added into extract solution
Enzyme and 2% pectase, stirring extraction 3h.
Enzyme digestion reaction adds the ethanol solution measured after terminating so that extraction conditions now are solid-to-liquid ratio 1：40th, second
Determining alcohol 70%, extraction vessel are warming up to extract solution temperature and reach 90 DEG C, continue stirring extraction 3h；Extraction terminates rear extract solution warp
Duplex strainer filters；The concentrated system vacuum of extract solution after filtering is concentrated under reduced pressure into no alcohol taste；Concentrate is spray-dried
Obtain aurantiamarin.
Detected through high performance liquid chromatography, the yield of aurantiamarin is 4.3% in obtained dried orange peel extracts.
Example IV
Traditional handicraft：Dried orange peel is crushed, a certain amount of Dried Tangerine Peel is accurately weighed and pours into extraction vessel, adds second
Alcoholic solution so that extraction conditions now are solid-to-liquid ratio 1：35th, concentration of alcohol 70%, extraction vessel are warming up to extract solution temperature and reached
To 90 DEG C, continue stirring extraction 2h；Extraction terminates rear extract solution and filtered through duplex strainer；The concentrated system of extract solution after filtering
Vacuum-concentrcted unite to without alcohol taste；Concentrate is spray-dried to obtain aurantiamarin.
Made from aurantiamarin yield and example IV traditional handicraft in dried orange peel extracts made from the embodiment of the present invention one
The comparing result of aurantiamarin yield in dried orange peel extracts see the table below：
| Project | Aurantiamarin yield (%) |
| Traditional alcohol extraction process | 3.71 ± 0.04% |
| Cellulase and pectin enzymatic process | 4.34 ± 0.06% |
The quality (mg) × 100% of content (mg)/dried orange peel of the aurantiamarin yield=aurantiamarin.
As can be seen from the above table, of the invention compared with traditional alcohol extraction process, the aurantiamarin yield in dried orange peel extracts improves
16.98%.
The present invention has efficiency high, easy to operate, reaction condition using the method for cellulase and pectase joint enzymolysis
The features such as gentle.Solvent extraction is used as using ethanol after enzyme digestion reaction, this method yield is high, safe and effective, and solvent is recyclable,
Suitable for industrialized production.
The above-described embodiments are merely illustrative of preferred embodiments of the present invention, not to the model of the present invention
Enclose and be defined, on the premise of design spirit of the present invention is not departed from, this area ordinary skill technical staff is to the technology of the present invention
The various modifications and improvement that scheme is made, it all should fall into the protection domain of claims of the present invention determination.
Claims (10)
- A kind of 1. method for preparing dried orange peel extracts, it is characterised in that enzyme-linked using cellulase and pectin using dried orange peel as raw material Synthase solution prepares dried orange peel extracts.
- 2. the method for dried orange peel extracts is prepared according to claim 1, it is characterised in that the cellulase and pectase Total amount accounts for the 2.5-4.0% of dried orange peel dosage.
- 3. the method for dried orange peel extracts is prepared according to claim 1, it is characterised in that the cellulase and pectase Match as 1:2-6.
- 4. the method for dried orange peel extracts is prepared according to claim 1, it is characterised in that comprise the following steps：Dried orange peel adds water to be warming up to 50-60 DEG C, and regulation pH value is 3.0-3.5, then adds the cellulase and pectin of special ratios Enzyme, enzyme digestion reaction is carried out, the reaction time is 2-3 hours, and the dried orange peel after enzymolysis is made；After the completion of the enzyme digestion reaction, ethanol solution is added into the dried orange peel solution after enzymolysis, is warming up to 80-90 DEG C, carries out alcohol Reaction is proposed, the reaction time is 2-3 hours, filtering, concentration, dries, dried orange peel extracts are made.
- 5. the method for dried orange peel extracts is prepared according to claim 4, it is characterised in that the proportioning of the dried orange peel and water is 1: 8-10。
- 6. the method for dried orange peel extracts is prepared according to claim 4, it is characterised in that using hydrochloric acid and/or sodium hydroxide Adjust pH value.
- 7. the method for dried orange peel extracts is prepared according to claim 4, it is characterised in that when alcohol extracting is reacted after the enzymolysis The proportioning of dried orange peel and ethanol solution is 1：22-30.
- 8. the dried orange peel extracts that according to claim 1 prepared by method.
- 9. dried orange peel extracts according to claim 8, it is characterised in that the yield of aurantiamarin is in the dried orange peel extracts 4.28-4.4%.
- 10. application of the dried orange peel extracts in liquid or solid health-care food is prepared described in claim 8.
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Application publication date: 20171205 |