CN107396956A - Reinforcing agent for casing and preparation method thereof - Google Patents
Reinforcing agent for casing and preparation method thereof Download PDFInfo
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- CN107396956A CN107396956A CN201710679254.4A CN201710679254A CN107396956A CN 107396956 A CN107396956 A CN 107396956A CN 201710679254 A CN201710679254 A CN 201710679254A CN 107396956 A CN107396956 A CN 107396956A
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- China
- Prior art keywords
- reinforcing agent
- casing
- parts
- polysaccharide
- auricularia
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- A—HUMAN NECESSITIES
- A22—BUTCHERING; MEAT TREATMENT; PROCESSING POULTRY OR FISH
- A22C—PROCESSING MEAT, POULTRY, OR FISH
- A22C13/00—Sausage casings
- A22C13/0013—Chemical composition of synthetic sausage casings
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention discloses the reinforcing agent for casing, the reinforcing agent includes following component：0.2 0.4 parts of transglutaminase, 0.1 0.2 parts of cysteine, 24 parts of Auricularia polysaccharide, 12 15 parts of glycerine.Invention also discloses the preparation method of the reinforcing agent for casing.Have the beneficial effect that：When the reinforcing agent is used for into collagen casing, the γ Carboxylamides group in amino acid residue can be promoted to crosslink effect with other amino acid residues, stronger network structure is formed by covalent bond, significantly improve the mechanical strength of collagen casing, the defects of casing easily ftractures in digestion process is overcome, and the reinforcing agent does not influence edibility, steam breathability, heat-shrinkable and the tackness to meat stuffing of casing.
Description
Technical field
The present invention relates to field of food, is especially for reinforcing agent of casing and preparation method thereof.
Background technology
At present, it is the Technology of Co-extruded Sausage to make a more advanced technology of sausage, and the technology is sausage processing industry
Important technology innovation, the key of sausage co-extrusion technology is " synchronous forming ", i.e., meat stuffing and membrane material is sub-packed in into two independences
Subsystem-meat stuffing filling machine and quantitatively send collagen machine, then being total to from meet-synchronous forming machines of the two subsystems
Squeeze at nozzle and be extruded simultaneously, collagen casing is constantly equably enclosed on the external table of intestines, and intestines body is from nozzle concentric circles internal layer
Sleeve opening is persistently extruded, and completes the synchronous forming of meat stuffing filling.It, which need not shut down, loads pre-processed casing, but
Continuously the gel of meat and casing is together extruded, is finally frozen into sausage.Wherein, the intensity of casing is processed for sausage coextrusion
Technology has important influence.
Prior art such as Authorization Notice No. is the B of CN 103524794 Chinese invention patent, discloses a kind of be applied to altogether
The enteric materials and its shaping dope of casing stripping technology, the casing reinforcing agent in the invention is sodium carboxymethylcellulose, strong to casing
Degree improves, but can not meet the needs of production.
The content of the invention
It is an object of the invention to provide a kind of reinforcing agent for casing, the reinforcing agent promotes amino in collagen casing
The crosslinking of acid, significantly improve the mechanical strength of collagen casing.
Another object of the present invention is to provide a kind of preparation for the reinforcing agent for casing that technique is simple, practicality is high
Method.
The present invention is directed to the problem of being mentioned in background technology for the present invention, and the technical scheme taken is：For casing
Reinforcing agent, the reinforcing agent include following component：Transglutaminase 0.2-0.4 parts, cysteine 0.1-0.2 parts, Auricularia polysaccharide
2-4 parts, glycerine 12-15 parts.When the reinforcing agent is used for into collagen casing, γ-Carboxylamide in amino acid residue can be promoted
Group crosslinks effect with other amino acid residues, forms stronger network structure by covalent bond, significantly improves collagen
The mechanical strength of casing, the defects of casing easily ftractures in digestion process is overcome, and the reinforcing agent does not influence the edible of casing
Property, steam breathability, heat-shrinkable and the tackness to meat stuffing.
Preferably, in the extraction process of Auricularia polysaccharide, 5-7 part agarics are taken, are crushed with medicinal herb grinder, add 7-10 parts
Water, 5-6min is stirred, be sent into homogenizer homogeneous 2-3min under 5000-6000r/min；Mixture obtained by homogeneous is heated to
92-98 DEG C, 40-50min is extracted, centrifuges 10-13min in the case where rotating speed is 8000-10000r/min, it is that polysaccharide carries to take supernatant
Take liquid.Using water as extraction polysaccharide, safety and environmental protection, cost is cheap, because agaric cell membrane has been abolished in homogenization, greatly
Intracellular polysaccharide is released, substantially increases the speed of extraction, in a short time can be to reach good extracting effect.
Preferably, in the purge process of Auricularia polysaccharide, 21-23 parts chloroform and positive fourth are added into polysaccharide extraction liquid
The volume ratio of the mixed liquor of alcohol, wherein chloroform and n-butanol is 3.4/1-3.7/1, stirs 32-40min, centrifuges, takes
Aqueous phase；22-25 parts 86%-92% ethanol solution is added into aqueous phase, stirs 16-25min, filtering, setting vacuum is 92-
100kPa, temperature are 60-70 DEG C, and filter residue and drying 25-35min is obtained into Auricularia polysaccharide.Solution obtained by hot water extraction's process contains
Many free proteins, after adding chloroform, protein can form precipitation and be separated out from solution, and then improve the pure of polysaccharide
Degree；Polysaccharide is dissolved in hot water and the ethanol insoluble in high concentration, and after 86%-92% ethanol solution is added, Auricularia polysaccharide is to precipitate
Form separated out from liquid phase, realize the separation of Auricularia polysaccharide and liquid phase, good bar provided for the practical application of polysaccharide
Part.
The preparation method of reinforcing agent in the present invention for casing is：0.2-0.4 parts are added into 12-15 part glycerine and turn paddy
Transglutaminase, 0.1-0.2 part cysteines, 2-4 part Auricularia polysaccharides, 15-18min is stirred, it is more to add 0.1-0.3 parts activity
Peptide, 5-10min is stirred, obtains reinforcing agent.Under the conditions of being somebody's turn to do, the various composition in reinforcing agent can be sufficiently mixed Bing and be uniformly dispersed, can
To improve reinforcing agent effect in use.
Preferably, the amino acid sequence of the active peptides added in the preparation process of reinforcing agent is：
HSHAYSCRCRRARRCGYYVSHRGRCYCRCLRCRVLHPGKLCVCVNCSR.The addition of active peptides can dramatically increase intestines
The intensity of clothing, it may be possible to because the oligonucleotide chain of active peptides and Auricularia polysaccharide by covalent bond forms a kind of glycoprotein, the sugared egg
The activity of transglutaminase can be improved in vain, and then promotes γ-carboxy and amide groups in collagen casing amino acid residue
Group crosslinks effect with other amino acid residues, improves the intensity of casing.
Compared with prior art, the advantage of the invention is that：Reinforcing agent in the present invention for casing is mainly by turning paddy ammonia
Amidase and Auricularia polysaccharide are formed, and the active peptides and the oligonucleotide chain of Auricularia polysaccharide added in preparation process pass through covalent key-shaped
Into a kind of glycoprotein, the glycoprotein can improve the activity of transglutaminase, and then promote collagen casing amino acid residual
γ-Carboxylamide group in base crosslinks effect with other amino acid residues, is formed by covalent bond stronger netted
Structure, the mechanical strength of collagen casing is significantly improved, overcomes the defects of casing easily ftractures in digestion process.
Embodiment
Below by embodiment, the present invention will be further described：
Embodiment 1：
For the reinforcing agent of casing, the reinforcing agent includes following component：0.2 part of transglutaminase, 0.1 part of cysteine, wood
2 parts of fungus polysaccharides, 12 parts of glycerine.The preparation method of the reinforcing agent comprises the following steps：
1) extraction of Auricularia polysaccharide：5 portions of agarics are taken, are crushed with medicinal herb grinder, add 7 parts of water, stir 5min, are sent into homogenizer
The homogeneous 2min under 5000r/min；Mixture obtained by homogeneous is heated to 92 DEG C, 40min is extracted, is 8000r/min in rotating speed
Lower centrifugation 10min, it is polysaccharide extraction liquid to take supernatant.Using water as extraction polysaccharide, safety and environmental protection, cost is cheap, due to
Agaric cell membrane has been abolished in homogenization, greatly releases intracellular polysaccharide, the speed of extraction is substantially increased, in short-term
In can be to reach good extracting effect；
2) purifying of Auricularia polysaccharide：The mixed liquor of 21 parts of chloroforms and n-butanol, wherein trichlorine are added into polysaccharide extraction liquid
The volume ratio of methane and n-butanol is 3.4/1, stirs 32min, is centrifuged, phase of fetching water；22 part 86% of second is added into aqueous phase
Alcoholic solution, 16min, filtering are stirred, setting vacuum is 92kPa, and temperature is 60 DEG C, and filter residue and drying 25min is obtained into Auricularia polysaccharide.
Solution obtained by hot water extraction's process contains many free proteins, and after adding chloroform, protein, which can be formed, to be precipitated from molten
Separated out in liquid, and then improve the purity of polysaccharide；Polysaccharide is dissolved in hot water and the ethanol insoluble in high concentration, is adding 86% second
After alcoholic solution, Auricularia polysaccharide is separated out in the form of precipitating from liquid phase, realizes the separation of Auricularia polysaccharide and liquid phase, is polysaccharide
Practical application provides good condition；
3) preparation of reinforcing agent：Into 12 parts of glycerine, 0.2 part of transglutaminase of addition, 0.1 part of cysteine, 2 portions of agarics are more
Sugar, 15min is stirred, add 0.1 part of active peptides, stirred 5min, obtain reinforcing agent.The amino acid sequence of the active peptides of addition
For：HSHAYSCRCRRARRCGYYVSHRGRCYCRCLRCRVLHPGKLCVCVNCSR.The addition of active peptides can significantly increase
Add the intensity of casing, it may be possible to, should because the oligonucleotide chain of active peptides and Auricularia polysaccharide by covalent bond forms a kind of glycoprotein
Glycoprotein can improve the activity of transglutaminase, and then promote γ-carboxyl acyl in collagen casing amino acid residue
Amine groups crosslink effect with other amino acid residues, improve the intensity of casing.
Embodiment 2：
For the reinforcing agent of casing, the reinforcing agent includes following component：0.4 part of transglutaminase, 0.2 part of cysteine, wood
4 parts of fungus polysaccharides, 15 parts of glycerine.The preparation method of the reinforcing agent comprises the following steps：It is the extraction of Auricularia polysaccharide first：Take 7 parts
Agaric, crush, add 10 parts of water, stir 6min, be sent into homogenizer homogeneous 3min under 6000r/min；It will be mixed obtained by homogeneous
Thing is heated to 98 DEG C, extracts 50min, centrifuges, it is polysaccharide extraction liquid to take supernatant；Followed by the purifying of Auricularia polysaccharide：
The mixed liquor of 23 parts of chloroforms and n-butanol is added into polysaccharide extraction liquid, the volume ratio of wherein chloroform and n-butanol is
3.7/1,40min is stirred, is centrifuged, phase of fetching water；25 part 92% of ethanol solution is added into aqueous phase, stirs 25min, is filtered,
Setting vacuum is 100kPa, and temperature is 70 DEG C, and filter residue and drying 35min is obtained into Auricularia polysaccharide；It is finally the preparation of reinforcing agent：To
0.4 part of transglutaminase is added in 15 parts of glycerine, 0.2 part of cysteine, 4 parts of Auricularia polysaccharides, 18min is stirred, adds
0.3 part of active peptides, 10min is stirred, obtains reinforcing agent.The amino acid sequence of the active peptides of addition is：
HSHAYSCRCRRARRCGYYVSHRGRCYCRCLRCRVLHPGKLCVCVNCSR.Under the conditions of being somebody's turn to do, the various composition in reinforcing agent
Bing can be sufficiently mixed to be uniformly dispersed, reinforcing agent effect in use can be improved.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
SEQUENCE LISTING
<110>Lanxi City victory eating product Processing Tech. Co., Ltd
<120>Reinforcing agent for casing and preparation method thereof
<130> 1
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 48
<212> PRT
<213>It is artificial synthesized
<400> 1
His Ser His Ala Tyr Ser Cys Arg Cys Arg Arg Ala Arg Arg Cys Gly
1 5 10 15
Tyr Tyr Val Ser His Arg Gly Arg Cys Tyr Cys Arg Cys Leu Arg Cys
20 25 30
Arg Val Leu His Pro Gly Lys Leu Cys Val Cys Val Asn Cys Ser Arg
35 40 45
Claims (6)
1. the reinforcing agent for casing, it is characterised in that：The reinforcing agent includes following component：Transglutaminase 0.2-0.4
Part, cysteine 0.1-0.2 parts, Auricularia polysaccharide 2-4 parts, glycerine 12-15 parts.
2. the reinforcing agent according to claim 1 for casing, it is characterised in that：The preparation of the Auricularia polysaccharide include with
Lower step：
1) extraction of Auricularia polysaccharide：5-7 part agarics are taken, are crushed with medicinal herb grinder, add 7-10 part water, 5-6min is stirred, send
Enter homogenizer；Mixture obtained by homogeneous is heated to 92-98 DEG C, extracts 40-50min, is centrifuged, it is polysaccharide to take supernatant
Extract solution；
2) purifying of Auricularia polysaccharide：The mixed liquor of 21-23 parts chloroform and n-butanol, stirring are added into polysaccharide extraction liquid
32-40min, centrifuge, phase of fetching water；22-25 parts 86%-92% ethanol solution is added into aqueous phase, stirs 16-25min, mistake
Filter, vacuum drying 25-35min obtain Auricularia polysaccharide.
3. the reinforcing agent according to claim 2 for casing, it is characterised in that：The rotating speed of homogenizer in the step 1)
For 5000-6000r/min, homogenizing time 2-3min.
4. the reinforcing agent according to claim 2 for casing, it is characterised in that：In the step 2) chloroform with just
The volume ratio of butanol is 3.4/1-3.7/1.
5. the preparation method of the reinforcing agent for casing, it is characterised in that：Methods described is：Added into 12-15 part glycerine
0.2-0.4 part transglutaminases, 0.1-0.2 part cysteines, 2-4 part Auricularia polysaccharides, 15-18min is stirred, adds 0.1-
0.3 part of active peptides, 5-10min is stirred, obtains reinforcing agent.
6. the preparation method of the reinforcing agent according to claim 5 for casing, it is characterised in that：The active peptides
Amino acid sequence is：HSHAYSCRCRRARRCGYYVSHRGRCYCRCLRCRVLHPGKLCVCVNCSR.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710679254.4A CN107396956A (en) | 2017-08-10 | 2017-08-10 | Reinforcing agent for casing and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710679254.4A CN107396956A (en) | 2017-08-10 | 2017-08-10 | Reinforcing agent for casing and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107396956A true CN107396956A (en) | 2017-11-28 |
Family
ID=60396356
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710679254.4A Pending CN107396956A (en) | 2017-08-10 | 2017-08-10 | Reinforcing agent for casing and preparation method thereof |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101610680A (en) * | 2006-12-08 | 2009-12-23 | 达特-施瓦博公司 | The method that connects the natural casing piece |
| CN101885920A (en) * | 2010-07-16 | 2010-11-17 | 中国海洋大学 | Method for preparing edible wrapping film |
| CN103518819A (en) * | 2013-11-01 | 2014-01-22 | 青岛明月海藻生物科技有限公司 | Sodium alga acid edible sausage casing and preparing method thereof |
| CN104628881A (en) * | 2015-03-05 | 2015-05-20 | 四川省农业科学院生物技术核技术研究所 | Method for extracting auricularia polytricha polysaccharide |
| CN104939269A (en) * | 2015-06-16 | 2015-09-30 | 宁波大学 | Edible composite sodium alginate antibacterial film liquid as well as preparation method and application thereof |
-
2017
- 2017-08-10 CN CN201710679254.4A patent/CN107396956A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101610680A (en) * | 2006-12-08 | 2009-12-23 | 达特-施瓦博公司 | The method that connects the natural casing piece |
| CN101885920A (en) * | 2010-07-16 | 2010-11-17 | 中国海洋大学 | Method for preparing edible wrapping film |
| CN103518819A (en) * | 2013-11-01 | 2014-01-22 | 青岛明月海藻生物科技有限公司 | Sodium alga acid edible sausage casing and preparing method thereof |
| CN104628881A (en) * | 2015-03-05 | 2015-05-20 | 四川省农业科学院生物技术核技术研究所 | Method for extracting auricularia polytricha polysaccharide |
| CN104939269A (en) * | 2015-06-16 | 2015-09-30 | 宁波大学 | Edible composite sodium alginate antibacterial film liquid as well as preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 姜燕等: "《多糖对酶法改性大豆蛋白膜性能的影响》", 《食品工业科技》 * |
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Application publication date: 20171128 |
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