CN107549269A - A kind of high intensity casing and preparation method thereof - Google Patents
A kind of high intensity casing and preparation method thereof Download PDFInfo
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- CN107549269A CN107549269A CN201710679283.0A CN201710679283A CN107549269A CN 107549269 A CN107549269 A CN 107549269A CN 201710679283 A CN201710679283 A CN 201710679283A CN 107549269 A CN107549269 A CN 107549269A
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Abstract
The invention discloses a kind of high intensity casing, the casing includes following component：0.2 0.4 parts of transglutaminase, 16 20 parts of collagen, 24 parts of Auricularia polysaccharide, 56 parts of glycerine, 13 18 parts of water.Invention also discloses a kind of preparation method of high intensity casing.Have the beneficial effect that：The oligonucleotide chain of active peptides and Auricularia polysaccharide forms a kind of glycoprotein by covalent bond, the glycoprotein can improve the activity of transglutaminase, and then the γ Carboxylamides group in collagen casing amino acid residue is promoted to crosslink effect with other amino acid residues, stronger network structure is formed by covalent bond, the mechanical strength and tensile strength of collagen casing are significantly improved, overcomes the defects of casing easily ftractures in digestion process.
Description
Technical field
The present invention relates to field of food, more particularly, to a kind of high intensity casing and preparation method thereof.
Background technology
At present, sausage casing foil can be made of a variety of materials, by prepare enteric materials origin classification can be divided into natural casing and
The major class of artificial casing two.Natural casing is pickled or the drying intestines formed after removing mucosa removal by pig, ox, the intestines of sheep and bladder
Clothing, it is characterized in that there is edibility, permeability, heat-shrinkable and the adherence to meat stuffing, but natural casing is diversified in specifications, by force
Degree is also uneven, and workability is poor when filling filling processing for machinery.Artificial collagen casing is with edible natural material-collagen
Albumen is primary raw material, by with other materials blending and modifying, by high pressure extrusion moulding capitiform into tubular article, then pass sequentially through
Drying is made after the processing such as solidification, crosslinking.Although the property of artificial collagen casing is very close with natural casing, it is also deposited
It is complicated in production method, production cost is higher, sausage boiling when the defects of easily ftractureing.
Prior art such as Authorization Notice No. is the B of CN 103518819 Chinese invention patent, discloses a kind of sodium alginate
Edibility casing and preparation method thereof, the casing in the invention is based on sodium alginate, edible gelatin and carboxymethyl cellulose sodium
Raw material is wanted, prepared casing intensity improves, but can not meet the needs of production.
The content of the invention
It is an object of the invention to provide a kind of high intensity casing, collagenic protein crosslinking degree is high in the casing, significantly
The tensile strength of casing is enhanced, meets the requirement of carnivorous bowel lavage, adds the application value of product.
Another object of the present invention is to provide a kind of preparation method for the high intensity casing that technique is simple, practicality is high.
The present invention is directed to the problem of being mentioned in background technology for the present invention, and the technical scheme taken is：A kind of high intensity
Casing, the casing include following component：Transglutaminase 0.2-0.4 parts, collagen 16-20 parts, Auricularia polysaccharide 2-4 parts,
Glycerine 5-6 parts, water 13-18 parts.γ-Carboxylamide group and other amino in the casing in collagenic protein amino acid residue
Sour residue crosslinks effect, forms stronger network structure by covalent bond, the tension for significantly improving collagen casing is strong
Degree and mechanical strength, the defects of casing easily ftractures in digestion process is overcome, and the casing also has good edibility, water
Vapour permeability, heat-shrinkable and the tackness to meat stuffing.
Preferably, in the extraction process of Auricularia polysaccharide, 5-7 part agarics are taken, are crushed with medicinal herb grinder, add 7-10 parts
Water, 5-6min is stirred, be sent into homogenizer homogeneous 2-3min under 5000-6000r/min；Mixture obtained by homogeneous is heated to
92-98 DEG C, 40-50min is extracted, centrifuges 10-13min in the case where rotating speed is 8000-10000r/min, it is that polysaccharide carries to take supernatant
Take liquid.Using water as extraction polysaccharide, safety and environmental protection, cost is cheap, because agaric cell membrane has been abolished in homogenization, greatly
Intracellular polysaccharide is released, substantially increases the speed of extraction, in a short time can be to reach good extracting effect.
Preferably, in the purge process of Auricularia polysaccharide, 21-23 parts chloroform and positive fourth are added into polysaccharide extraction liquid
The volume ratio of the mixed liquor of alcohol, wherein chloroform and n-butanol is 3.4/1-3.7/1, stirs 32-40min, centrifuges, takes
Aqueous phase；22-25 parts 86%-92% ethanol solution is added into aqueous phase, stirs 16-25min, filtering, setting vacuum is 92-
100kPa, temperature are 60-70 DEG C, and filter residue and drying 25-35min is obtained into Auricularia polysaccharide.Solution obtained by hot water extraction's process contains
Many free proteins, after adding chloroform, protein can form precipitation and be separated out from solution, and then improve the pure of polysaccharide
Degree；Polysaccharide is dissolved in hot water and the ethanol insoluble in high concentration, and after 86%-92% ethanol solution is added, Auricularia polysaccharide is to precipitate
Form separated out from liquid phase, realize the separation of Auricularia polysaccharide and liquid phase, good bar provided for the practical application of polysaccharide
Part.
A kind of preparation method of high intensity casing is in the present invention：16-20 parts collagen and 0.2-0.4 parts is taken to turn paddy ammonia
Amidase, 13-18 parts water and 5-6 part glycerine being added, the pH for controlling solution is 7-8, and temperature is 35-40 DEG C, stirs 10-15min,
0.1-0.2 parts active peptides and 2-4 part Auricularia polysaccharides are added, stir 5-7min, mixed liquor are injected in disk, in 35-40
Dried at DEG C, obtain casing.Process is simple, and the casing steady quality of preparation, thickness is uniform, and tensile property is good, has good
Elongation at break, the cost of raw material used is cheap, and ratio is moderate between various composition, can be fully anti-in preparation process
Answer, improve the utilization rate of resource.
Preferably, the amino acid sequence of the active peptides added in the preparation process of casing is：
HSHACSCRCRRARRCGYYVSHRGRCYCRCLRCRVLHPGKLCVCVNCSR.The addition of active peptides can significantly improve intestines
The tensile property of clothing, it may be possible to, should because the oligonucleotide chain of active peptides and Auricularia polysaccharide by covalent bond forms a kind of glycoprotein
Glycoprotein can improve the activity of transglutaminase, and then promote γ-carboxyl acyl in collagen casing amino acid residue
Amine groups crosslink effect with other amino acid residues, improve the adhesion between casing amino acid.
Compared with prior art, the advantage of the invention is that：A kind of high intensity casing is mainly by turning glutamy in the present invention
Amine enzyme, Auricularia polysaccharide and active peptides are formed, and the oligonucleotide chain of active peptides and Auricularia polysaccharide forms a kind of sugar by covalent bond
Albumen, the glycoprotein can improve the activity of transglutaminase, so promote γ in collagen casing amino acid residue-
Carboxylamide group crosslinks effect with other amino acid residues, forms stronger network structure by covalent bond, significantly
The mechanical strength and tensile strength of collagen casing are improved, overcomes the defects of casing easily ftractures in digestion process.
Embodiment
Below by embodiment, the present invention will be further described：
Embodiment 1：
A kind of high intensity casing, the casing include following component：0.2 part of transglutaminase, 16 parts of collagen, Auricularia polysaccharide
2 parts, 5 parts of glycerine, 13 parts of water.The preparation method of the casing comprises the following steps：
1) extraction of Auricularia polysaccharide：5 portions of agarics are taken, are crushed with medicinal herb grinder, add 7 parts of water, stir 5min, are sent into homogenizer
The homogeneous 2min under 6000r/min；Mixture obtained by homogeneous is heated to 92 DEG C, 40min is extracted, is 8000r/min in rotating speed
Lower centrifugation 10min, it is polysaccharide extraction liquid to take supernatant.Using water as extraction polysaccharide, safety and environmental protection, cost is cheap, due to
Agaric cell membrane has been abolished in homogenization, greatly releases intracellular polysaccharide, the speed of extraction is substantially increased, in short-term
In can be to reach good extracting effect；
2) purifying of Auricularia polysaccharide：The mixed liquor of 21 parts of chloroforms and n-butanol, wherein trichlorine are added into polysaccharide extraction liquid
The volume ratio of methane and n-butanol is 3.4/1, stirs 32min, is centrifuged, phase of fetching water；22 part 86% of second is added into aqueous phase
Alcoholic solution, 16min, filtering are stirred, setting vacuum is 92kPa, and temperature is 60 DEG C, and filter residue and drying 25min is obtained into Auricularia polysaccharide.
Solution obtained by hot water extraction's process contains many free proteins, and after adding chloroform, protein, which can be formed, to be precipitated from molten
Separated out in liquid, and then improve the purity of polysaccharide；Polysaccharide is dissolved in hot water and the ethanol insoluble in high concentration, is adding 86% second
After alcoholic solution, Auricularia polysaccharide is separated out in the form of precipitating from liquid phase, realizes the separation of Auricularia polysaccharide and liquid phase, is polysaccharide
Practical application provides good condition；
3) preparation of casing：16 parts of collagens and 0.2 part of transglutaminase are taken, add 13 parts of water and 5 parts of glycerine, control is molten
The pH of liquid is 7, and temperature is 35 DEG C, stirs 10min, adds 0.1 part of active peptides and 2 parts of Auricularia polysaccharides, stirs 5min, will be mixed
Close in liquid injection disk, dried at 35 DEG C, obtain casing.The amino acid sequence of the active peptides of addition is：
HSHACSCRCRRARRCGYYVSHRGRCYCRCLRCRVLHPGKLCVCVNCSR.The addition of active peptides can significantly improve intestines
The tensile property of clothing, it may be possible to, should because the oligonucleotide chain of active peptides and Auricularia polysaccharide by covalent bond forms a kind of glycoprotein
Glycoprotein can improve the activity of transglutaminase, and then promote γ-carboxyl acyl in collagen casing amino acid residue
Amine groups crosslink effect with other amino acid residues, improve the adhesion between casing amino acid.
Embodiment 2：
A kind of high intensity casing, the casing include following component：0.4 part of transglutaminase, 20 parts of collagen, Auricularia polysaccharide
4 parts, 6 parts of glycerine, 18 parts of water.The preparation method of the casing comprises the following steps：It is the extraction of Auricularia polysaccharide first：Take 7 parts of wood
Ear, crush, add 10 parts of water, stir 6min, be sent into homogenizer homogeneous 3min under 5000r/min；By mixture obtained by homogeneous
98 DEG C are heated to, extracts 50min, is centrifuged, it is polysaccharide extraction liquid to take supernatant；Followed by the purifying of Auricularia polysaccharide：To
The mixed liquor of 23 parts of chloroforms and n-butanol is added in polysaccharide extraction liquid, the volume ratio of wherein chloroform and n-butanol is
3.7/1,40min is stirred, is centrifuged, phase of fetching water；25 part 92% of ethanol solution is added into aqueous phase, stirs 25min, is filtered,
Setting vacuum is 100kPa, and temperature is 70 DEG C, and filter residue and drying 35min is obtained into Auricularia polysaccharide；It is finally the preparation of casing：Take 20
Part collagen and 0.4 part of transglutaminase, 18 parts of water and 6 parts of glycerine being added, the pH for controlling solution is 8, and temperature is 40 DEG C,
15min is stirred, adds 0.2 part of active peptides and 4 parts of Auricularia polysaccharides, stirs 7min, mixed liquor is injected in disk, at 40 DEG C
Lower drying, obtains casing.The amino acid sequence of the active peptides of addition is：
HSHACSCRCRRARRCGYYVSHRGRCYCRCLRCRVLHPGKLCVCVNCSR.Process is simple, the casing quality of preparation
Stable, thickness is uniform, and tensile property is good, has good elongation at break, and the cost of raw material used is cheap, various composition it
Between ratio it is moderate, can fully be reacted in preparation process, improve the utilization rate of resource.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
SEQUENCE LISTING
<110>Pujiang County is safe such as food science and technology Co., Ltd
<120>A kind of high intensity casing and preparation method thereof
<130> 1
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 48
<212> PRT
<213>It is artificial synthesized
<400> 1
His Ser His Ala Cys Ser Cys Arg Cys Arg Arg Ala Arg Arg Cys Gly
1 5 10 15
Tyr Tyr Val Ser His Arg Gly Arg Cys Tyr Cys Arg Cys Leu Arg Cys
20 25 30
Arg Val Leu His Pro Gly Lys Leu Cys Val Cys Val Asn Cys Ser Arg
35 40 45
Claims (6)
- A kind of 1. high intensity casing, it is characterised in that：The casing includes following component：Transglutaminase 0.2-0.4 parts, glue Former -20 parts of protein 16, Auricularia polysaccharide 2-4 parts, glycerine 5-6 parts, water 13-18 parts.
- A kind of 2. high intensity casing according to claim 1, it is characterised in that：The preparation of the Auricularia polysaccharide includes following Step：1) extraction of Auricularia polysaccharide：5-7 part agarics are taken, are crushed with medicinal herb grinder, add 7-10 part water, 5-6min is stirred, send Enter homogenizer；Mixture obtained by homogeneous is heated to 92-98 DEG C, extracts 40-50min, is centrifuged, it is polysaccharide to take supernatant Extract solution；2) purifying of Auricularia polysaccharide：The mixed liquor of 21-23 parts chloroform and n-butanol, stirring are added into polysaccharide extraction liquid 32-40min, centrifuge, phase of fetching water；22-25 parts 86%-92% ethanol solution is added into aqueous phase, stirs 16-25min, mistake Filter, vacuum drying 25-35min obtain Auricularia polysaccharide.
- A kind of 3. high intensity casing according to claim 2, it is characterised in that：The rotating speed of homogenizer is in the step 1) 5000-6000r/min, homogenizing time 2-3min.
- A kind of 4. high intensity casing according to claim 2, it is characterised in that：Chloroform and positive fourth in the step 2) The volume ratio of alcohol is 3.4/1-3.7/1.
- A kind of 5. preparation method of high intensity casing, it is characterised in that：Methods described is：Take 16-20 parts collagen and 0.2- 0.4 part of transglutaminase, 13-18 parts water and 5-6 part glycerine are added, the pH for controlling solution is 7-8, and temperature is 35-40 DEG C, is stirred 10-15min is mixed, adds 0.1-0.2 parts active peptides and 2-4 part Auricularia polysaccharides, 5-7min is stirred, mixed liquor is injected into disk In, dried at 35-40 DEG C, obtain casing.
- A kind of 6. preparation method of high intensity casing according to claim 5, it is characterised in that：The ammonia of the active peptides Base acid sequence is：HSHACSCRCRRARRCGYYVSHRGRCYCRCLRCRVLHPGKLCVCVNCSR.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710679283.0A CN107549269A (en) | 2017-08-10 | 2017-08-10 | A kind of high intensity casing and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710679283.0A CN107549269A (en) | 2017-08-10 | 2017-08-10 | A kind of high intensity casing and preparation method thereof |
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| CN201710679283.0A Withdrawn CN107549269A (en) | 2017-08-10 | 2017-08-10 | A kind of high intensity casing and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109907249A (en) * | 2018-11-22 | 2019-06-21 | 福建御冠食品有限公司 | A kind of genuine intestines and preparation method thereof |
| CN112244069A (en) * | 2020-10-22 | 2021-01-22 | 陈强 | Casing for sausages and preparation method thereof |
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|---|---|---|---|---|
| CN101731305A (en) * | 2009-12-23 | 2010-06-16 | 杜学仲 | Method for manufacturing edible artificial sausage skin from soybean proteins |
| CN101885920A (en) * | 2010-07-16 | 2010-11-17 | 中国海洋大学 | Method for preparing edible wrapping film |
| CN103518819B (en) * | 2013-11-01 | 2015-04-15 | 青岛明月海藻生物科技有限公司 | Sodium alga acid edible sausage casing and preparing method thereof |
| CN104628881A (en) * | 2015-03-05 | 2015-05-20 | 四川省农业科学院生物技术核技术研究所 | Method for extracting auricularia polytricha polysaccharide |
| CN105384837A (en) * | 2015-12-16 | 2016-03-09 | 黑龙江众生生物工程有限公司 | Microwave-supersonic wave-combined auricularia auriculajudae polysaccharide extraction method |
| CN106472648A (en) * | 2016-10-07 | 2017-03-08 | 邯郸帝科胶原蛋白肠衣有限公司 | A kind of edible collagen casing and preparation method thereof |
| CN106749724A (en) * | 2016-12-09 | 2017-05-31 | 昭平县科学技术指导站 | A kind of method for extracting Auricularia polysaccharide |
-
2017
- 2017-08-10 CN CN201710679283.0A patent/CN107549269A/en not_active Withdrawn
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101731305A (en) * | 2009-12-23 | 2010-06-16 | 杜学仲 | Method for manufacturing edible artificial sausage skin from soybean proteins |
| CN101885920A (en) * | 2010-07-16 | 2010-11-17 | 中国海洋大学 | Method for preparing edible wrapping film |
| CN103518819B (en) * | 2013-11-01 | 2015-04-15 | 青岛明月海藻生物科技有限公司 | Sodium alga acid edible sausage casing and preparing method thereof |
| CN104628881A (en) * | 2015-03-05 | 2015-05-20 | 四川省农业科学院生物技术核技术研究所 | Method for extracting auricularia polytricha polysaccharide |
| CN105384837A (en) * | 2015-12-16 | 2016-03-09 | 黑龙江众生生物工程有限公司 | Microwave-supersonic wave-combined auricularia auriculajudae polysaccharide extraction method |
| CN106472648A (en) * | 2016-10-07 | 2017-03-08 | 邯郸帝科胶原蛋白肠衣有限公司 | A kind of edible collagen casing and preparation method thereof |
| CN106749724A (en) * | 2016-12-09 | 2017-05-31 | 昭平县科学技术指导站 | A kind of method for extracting Auricularia polysaccharide |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109907249A (en) * | 2018-11-22 | 2019-06-21 | 福建御冠食品有限公司 | A kind of genuine intestines and preparation method thereof |
| CN112244069A (en) * | 2020-10-22 | 2021-01-22 | 陈强 | Casing for sausages and preparation method thereof |
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Application publication date: 20180109 |