CN107164440A - High F value oligopeptide Coarse liquid separation purification process - Google Patents
High F value oligopeptide Coarse liquid separation purification process Download PDFInfo
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Abstract
The invention provides a kind of high F value oligopeptide Coarse liquid separation purification process, operating procedure is：1）Pretreatment；2）Enzymolysis；3）Ultrafiltration；4）The de- virtue of activated carbon；5）Gel chromatography；6）HPLC is purified.Have the beneficial effect that：Activated carbon advantage of lower cost, will not also produce pollution in process of production, easily reclaim.Due to the outer telogenesis irregular shape of granular activated carbon, pore structure is more complicated, to free aromatic amino acid adsorption efficiency preferably, and de- virtue rate is higher, while good deodorant effect can also be played, is effectively improved the quality of high F value oligopeptide；Dopen Nano aluminum oxide and nano-sized iron oxide can increase its adsorption rate to free aromatic amino acid in activated carbon, improve oligopeptides F values；The isolation and purification method of the present invention, with low cost, simple to operate, green safety is adapted to large-scale production.
Description
Technical field
The present invention relates to peptide separation technical field of purification, specifically a kind of high F value oligopeptide Coarse liquid separation purification process.
Background technology
Hairtail（Largehead Hairtail）, nickname hairtail, dental strip fish belong to Chordata, Perciformes, build is in
Banding.Western Pacific and the Indian Ocean are distributed mainly on, is all distributed up to the South Sea in the Huanghai Sea of China, the East Sea, the Bohai Sea, and greatly
Yellow croaker, little yellow croaker and cuttlefish and the four big marine products for being referred to as China.
2014 years, Zhoushan hairtail quantity of the catch reaches 108104 tons, and the ratio for accounting for main fishery harvesting kind reaches 11.52%.
Zhoushan hairtail is more with fresh or be processed into various fish products and sell, but the exploitation of hairtail are still in the roughing stage,
The producing level of high added value is relatively low.
The average protein content of hairtail reaches more than 18.4%, and this causes it to turn into abundant protein starting material source.Egg
White matter can prepare the biologically active peptide for possessing the functions such as some special physiologicals, health care by specially treateds such as enzymolysis.At present,
Although China have made some progress to the research with Fish protein, but also fails to develop the albumen of high activity, also fails to more
It is good fully to develop band fish resource.Therefore, to the further investigation with Fish protein, the hairtail egg for possessing high added value is developed
The problem of white product turns into extremely urgent.
High F value oligopeptide is the small peptide mixer that a class is combined into by 2-9 amino acid in the way of unique, its principal character
It is that branched-chain amino acid is far above Human Body Model with aromatic amino acid ratio in ispol.Figured silk fabrics ammonia in branched-chain amino acid
Acid（Val）Isoleucine（Ile）Leucine（Leu）Hinder with safeguarding normal nervous system, increase appetite, treating anaemia, promotion
The special physiological functions such as mouth recovery from illness.Clinically, high F value oligopeptide is commonly used to for treating hepatic encephalopathy, and PKU changes
Postoperative nutrition condition of kind patient etc..In recent years, the study hotspot of functional food is turned into terms of domestic high F values oligopeptides exploitation.Such as
Using plants such as soybean, corn, peanuts as raw material, the high F value oligopeptide prepared by raw material of aquatic products such as tuna, squid, oysters.
The developmental research for hairtail high F value oligopeptide is only in the exploration of laboratory level at present, and manufactured goods on the market are very few.
At present, the high F value oligopeptide method for preparing of more popular and science is using double enzyme distribution enzymatic isolation methods.Its main technique
It is that the aromatic amino acid residue site of protein peptide chain is exposed by enzymolysis first, then with some specific proteases enzymes
Solution cuts away aromatic amino acid, is allowed to exist in solution with free state, then maximizes removal aromatic series amino with other method
Acid.The key for preparing high F oligopeptides is to retain branched-chain amino acid as far as possible（Val, Ile, Leu abbreviation BCAA）, remove aromatic series
Amino acid（Tyr, Phe, abbreviation AAA）, reach that BCAA/AAA molar concentration rate is more than 20.
Prior art such as Authorization Notice No. is CN104004087B Chinese invention patent, discloses a kind of leech high F value
Oligopeptides and its enzymolysis preparation and application, preparation method include：（1）Leech stem body tentatively cleans removal of impurities, crushes at a high speed；（2）
Raw material alkali pre-soaking；（3）Alkali protease Alcalase AF 2.4L combination papain double-enzyme hydrolysis；（4）Using activity
Powdered carbon Static Adsorption removes aromatic amino acid；（5）Further isolated and purified with gel permeation chromatography post, collect separation product,
Freeze-drying, obtains molecular weight and is less than 2000Da, F values（Fischer ratio, i.e. branched-chain amino acid rub with aromatic amino acid
The ratio of your content）Leech oligopeptides powder higher than 20.This method preparation process is simple, from alkali protease and papain
Two kinds of business enzymes, it is easy to which industrial production, yield is higher, are added with certain anticoagulant effect, and for high F value class product
New sources.The free aromatic amino acid clearance rate of this method is not ideal, and the oligopeptides F finally obtained is not very high.
The content of the invention
Simple to operate it is an object of the invention to provide one kind is with low cost, it is low to obtain aromatic amino acid content, branch
Chain amino acid retention rate is high, i.e. the oligopeptides Coarse liquid separation purification process of high F value.
The present invention is directed to the problem of being mentioned in background technology, and the technical scheme taken is：
High F value oligopeptide Coarse liquid separation purification process, is concretely comprised the following steps：
Pretreatment：By hairtail decaptitating, remove the peel, remove internal organ, rinsed well, be cut into small pieces with clear water, by volume 1:4 ~ 8 additions are gone
Raw meat liquid, deodorization liquid composition and its parts by weight are：5 ~ 10 parts of citric acids, 10 ~ 18 parts of extractive of perilla, 0.1 ~ 0.3 part of sodium chloride,
0.5 ~ 0.8 part of sodium acid carbonate, 0.001 ~ 0.003 part of malol, 20 ~ 30 parts of ethanol, 0.02 ~ 0.04 part of Kaempferitrin and 0.2 ~ 0.4
Part vitamin E.Immersion, soak time is 4 ~ 8h.Smash to pieces, sieve, by volume 1:4.5 ~ 5.2 add ethyl acetate, stir, and take off
The fat time is 20 ~ 25h.Filtering, by volume 1 in filter residue:2 ~ 4 add deionized water, remove grease, filtering, in 42 ~ 48 DEG C of rings
Dried under border, obtain degreasing hairtail powder；
Enzymolysis：By volume 1 in hairtail powder:4.6 ~ 5.3 add distilled water, adjust pH, add pepsin and enzyme activition
Agent, pepsin zymoexciter composition and its parts by weight are：0.002 ~ 0.004 portion of propyl acetate and 0.01 ~ 0.04 part of vitamin
B1.The enzyme concentration of pepsin is that 2.8 ~ 3.3%, pH is 1.5 ~ 2.3, hydrolysis temperature be 35-37 DEG C, enzymolysis time be 1.8 ~
2.6h.Enzymolysis terminates rear boiling water bath and gone out 5 ~ 10min of enzyme, adjusts pH, adds flavor protease and zymoexciter, food flavor enzyme enzyme activition
Agent composition and its parts by weight are：0.03 ~ 0.05 part of magnesium acetate and 0.001 ~ 0.003 part of dimethyl carbonate.Flavor protease plus
Enzyme amount is that 2.8 ~ 3.3%, pH is 6.4 ~ 7.2, and hydrolysis temperature is 48 ~ 52 DEG C, and enzymolysis time is 1.2 ~ 1.9h.Enzymolysis boils after terminating
Water-bath is gone out 5 ~ 10min of enzyme, is filtrated to get the thick liquid of high F value oligopeptide；
Ultrafiltration：The enzymolysis liquid ultrafiltration that step 2 is obtained, milipore filter specification is 4.5 ~ 5.5kDa, obtains filtered solution.High F value oligopeptide
Generally there is 2-9 amino acid residue, mean molecule quantity is less than 1.5kDa, and ultrafiltration can remove macro-molecular protein impurity, and high F value is few
Peptide is purified；
The de- virtue of activated carbon：Add activated carbon in filtered solution, activated carbon is be made by raw material of high-quality cocoanut shell powdered activated
Charcoal, activated carbon 0.2 ~ 0.45% nano aluminium oxide of doping and 0.1 ~ 0.3% nano-sized iron oxide.Stirring, activated carbon and filtered solution solid-liquid
Than for 1:18 ~ 23, pH are 5.5 ~ 6.5, and temperature is 22 ~ 26 DEG C, and adsorption time is 2.5 ~ 3.2h.Centrifugation, supernatant is freezed dry
It is dry to obtain oligopeptide powder.Activated carbon advantage of lower cost, will not also produce pollution in process of production, easily reclaim.Due to
The outer telogenesis irregular shape of grain activated carbon, pore structure is more complicated, to free aromatic amino acid adsorption efficiency preferably, takes off virtue
Rate is higher, while good deodorant effect can also be played, is effectively improved the quality of high F value oligopeptide；Adulterated in activated carbon
Nano aluminium oxide and nano-sized iron oxide can increase its adsorption rate to free aromatic amino acid, improve oligopeptides F values；
Gel chromatography：SepHadex G-25 gels are placed in distilled water and soaked, soaking temperature is 50 ~ 65 DEG C, soak time is
5~6.5h.Bottle,suction is poured into after being fully swelled, is vacuumized, during which, water is constantly changed and removes and swim in the impurity on surface, sop up
Bubble in gel particle, until supernatant is as clear as crystal, have no any suspension after gel precipitation.Fill post, balance, by oligopeptides
Powder is dissolved in distilled water, oligopeptide powder：Distilled water mass volume ratio is 18mg：1mL~22mg：Excessively 0.42 after 1mL, fully dissolving ~
0.48 μm of needle cylinder type filter membrane, takes pressure adsorption loading, treats that sample is just fully flowed into post, switches to elution mode,
Start elution by mobile phase of ultra-pure water, flow velocity is 0.54 ~ 0.65 mL/min, and every 1.8 ~ 2.2min collects 1 and managed.Detection is often managed
Ultraviolet absorption value of the sample at 220nm and 280nm, sample is merged by peak and collected, freeze-drying.SepHadex G-25 coagulate
Glue particle, has good separating effect for small peptide and other small molecules, and appearance peak type is stable.Moreover, SepHade gels
Grain has preferable heat resistance, stability in weak acid, aqueous slkali and organic solution, may be reused after overactivation,
Also there is certain desalination function；
HPLC is purified：Using liquid chromatograph, acetonitrile and ultra-pure water are that eluent carries out gradient elution, elution time, eluent
Composition and its percentage and flow velocity are followed successively by：0 ~ 5min, acetonitrile 15%, ultra-pure water 85%, flow velocity is 0.45 ~ 0.52 mL/min；5~
15min, acetonitrile 40%, ultra-pure water 60%, flow velocity is 0.45 ~ 0.52 mL/min；15 ~ 25min, acetonitrile 80%, ultra-pure water 20%, stream
Speed is 0.45 ~ 0.52 mL/min；25 ~ 30min, acetonitrile 100%, flow velocity is 0.55 ~ 0.62 mL/min；30 ~ 40min, acetonitrile
40%, ultra-pure water 60%, flow velocity is 0.55 ~ 0.62 mL/min；40 ~ 50min, acetonitrile 20%, ultra-pure water 80%, flow velocity be 0.55 ~
0.62 mL/min.Collect eluent, freeze-drying.The F values of the oligopeptides finally obtained can be up to more than 40.The thick liquid of high F value oligopeptide
In contain amino acid sequence be SCASVCKA high F value oligopeptide.
Compared with prior art, the advantage of the invention is that：High F value oligopeptide generally has 2-9 amino acid residue, average mark
Son amount is less than 1.5kDa, and ultrafiltration can remove macro-molecular protein impurity, and high F value oligopeptide is purified；Activated carbon cost is relatively
It is low, pollution will not be also produced in process of production, easily reclaimed.Due to the outer telogenesis irregular shape of granular activated carbon, hole knot
Structure is more complicated, to free aromatic amino acid adsorption efficiency preferably, and de- virtue rate is higher, while good deodorant can also be played
Effect, is effectively improved the quality of high F value oligopeptide；Can to increase its right for dopen Nano aluminum oxide and nano-sized iron oxide in activated carbon
The adsorption rate of free aromatic amino acid, improves oligopeptides F values；SepHadex G-25 gel particles are small with other for small peptide
Molecule has good separating effect, and appearance peak type is stable.Moreover, SepHade gel particles are in weak acid, aqueous slkali and organic
There is preferable heat resistance, stability in solution, may be reused after overactivation, also with certain desalination function；HPLC
The F values of the oligopeptides obtained after purification can be up to more than 40；The isolation and purification method of the present invention, with low cost, simple to operate, green
Safety, is adapted to large-scale production.
Specific embodiment
The present invention program is described further below by embodiment：
Embodiment 1：
High F value oligopeptide Coarse liquid separation purification process, is concretely comprised the following steps：
1）Pretreatment：By hairtail decaptitating, remove the peel, remove internal organ, rinsed well, be cut into small pieces with clear water, by volume 1:4 ~ 8 add
Deodorization liquid, deodorization liquid composition and its parts by weight are：5 ~ 10 parts of citric acids, 10 ~ 18 parts of extractive of perilla, 0.1 ~ 0.3 part of sodium chloride,
0.5 ~ 0.8 part of sodium acid carbonate, 0.001 ~ 0.003 part of malol, 20 ~ 30 parts of ethanol, 0.02 ~ 0.04 part of Kaempferitrin and 0.2 ~ 0.4
Part vitamin E.Immersion, soak time is 4 ~ 8h.Smash to pieces, sieve, by volume 1:4.5 ~ 5.2 add ethyl acetate, stir, and take off
The fat time is 20 ~ 25h.Filtering, by volume 1 in filter residue:2 ~ 4 add deionized water, remove grease, filtering, in 42 ~ 48 DEG C of rings
Dried under border, obtain degreasing hairtail powder；
2）Enzymolysis：By volume 1 in hairtail powder:4.6 ~ 5.3 add distilled water, adjust pH, add pepsin and enzyme swashs
Agent living, pepsin zymoexciter composition and its parts by weight are：0.002 ~ 0.004 portion of propyl acetate and 0.01 ~ 0.04 part of dimension life
Plain B1.The enzyme concentration of pepsin is that 2.8 ~ 3.3%, pH is 1.5 ~ 2.3, hydrolysis temperature be 35-37 DEG C, enzymolysis time be 1.8 ~
2.6h.Enzymolysis terminates rear boiling water bath and gone out 5 ~ 10min of enzyme, adjusts pH, adds flavor protease and zymoexciter, food flavor enzyme enzyme activition
Agent composition and its parts by weight are：0.03 ~ 0.05 part of magnesium acetate and 0.001 ~ 0.003 part of dimethyl carbonate.Flavor protease plus
Enzyme amount is that 2.8 ~ 3.3%, pH is 6.4 ~ 7.2, and hydrolysis temperature is 48 ~ 52 DEG C, and enzymolysis time is 1.2 ~ 1.9h.Enzymolysis boils after terminating
Water-bath is gone out 5 ~ 10min of enzyme, is filtrated to get the thick liquid of high F value oligopeptide；
3）Ultrafiltration：The enzymolysis liquid ultrafiltration that step 2 is obtained, milipore filter specification is 4.5 ~ 5.5kDa, obtains filtered solution.High F value is few
Peptide generally has 2-9 amino acid residue, and mean molecule quantity is less than 1.5kDa, and ultrafiltration can remove macro-molecular protein impurity, high F value
Oligopeptides is purified；
4）The de- virtue of activated carbon：Activated carbon is added in filtered solution, activated carbon is that the powdery being made using high-quality cocoanut shell as raw material is lived
Property charcoal, activated carbon adulterates 0.2 ~ 0.45% nano aluminium oxide and 0.1 ~ 0.3% nano-sized iron oxide.Stirring, activated carbon and filtered solution are solid
Liquor ratio is 1:18 ~ 23, pH are 5.5 ~ 6.5, and temperature is 22 ~ 26 DEG C, and adsorption time is 2.5 ~ 3.2h.Centrifugation, supernatant is freezed
It is dried to obtain oligopeptide powder.Activated carbon advantage of lower cost, will not also produce pollution in process of production, easily reclaim.Due to
The outer telogenesis irregular shape of granular activated carbon, pore structure is more complicated, to free aromatic amino acid adsorption efficiency preferably, takes off
Fragrant rate is higher, while good deodorant effect can also be played, is effectively improved the quality of high F value oligopeptide；Mixed in activated carbon
Miscellaneous nano aluminium oxide and nano-sized iron oxide can increase its adsorption rate to free aromatic amino acid, improve oligopeptides F values；
5）Gel chromatography：SepHadexG-25 gels are placed in distilled water and soaked, soaking temperature is 50 ~ 65 DEG C, soak time
For 5 ~ 6.5h.Bottle,suction is poured into after being fully swelled, is vacuumized, during which, water is constantly changed and removes the impurity for swimming in surface, suction
Fall the bubble in gel particle, until supernatant is as clear as crystal, have no any suspension after gel precipitation.Fill post, balance, by widow
Peptide powder is dissolved in distilled water, oligopeptide powder：Distilled water mass volume ratio is 18mg：1mL~22mg：Mistake after 1mL, fully dissolving
0.42 ~ 0.48 μm of needle cylinder type filter membrane, takes pressure adsorption loading, treats that sample is just fully flowed into post, switches to elution
Pattern, starts elution by mobile phase of ultra-pure water, and flow velocity is 0.54 ~ 0.65 mL/min, and every 1.8 ~ 2.2min collects 1 and managed.Detection
Often ultraviolet absorption value of the pipe sample at 220nm and 280nm, sample is merged by peak and collected, freeze-drying.SepHadex G-
25 gel particles, have good separating effect for small peptide and other small molecules, and appearance peak type is stable.Moreover, SepHade is solidifying
Glue particle has preferable heat resistance, stability in weak acid, aqueous slkali and organic solution, can repeat to make after overactivation
With also with certain desalination function；
6）HPLC is purified：Using liquid chromatograph, acetonitrile and ultra-pure water are that eluent carries out gradient elution, elution time, elution
Liquid composition and its percentage and flow velocity are followed successively by：0 ~ 5min, acetonitrile 15%, ultra-pure water 85%, flow velocity is 0.45 ~ 0.52 mL/min；
5 ~ 15min, acetonitrile 40%, ultra-pure water 60%, flow velocity is 0.45 ~ 0.52 mL/min；15 ~ 25min, acetonitrile 80%, ultra-pure water 20%,
Flow velocity is 0.45 ~ 0.52 mL/min；25 ~ 30min, acetonitrile 100%, flow velocity is 0.55 ~ 0.62 mL/min；30 ~ 40min, acetonitrile
40%, ultra-pure water 60%, flow velocity is 0.55 ~ 0.62 mL/min；40 ~ 50min, acetonitrile 20%, ultra-pure water 80%, flow velocity be 0.55 ~
0.62 mL/min.Collect eluent, freeze-drying.The F values of the oligopeptides finally obtained can be up to more than 40.
Embodiment 2：
High F value oligopeptide Coarse liquid separation purification process, most preferably step is：
1）Pretreatment：By hairtail decaptitating, remove the peel, remove internal organ, rinsed well with clear water, be cut into the long fritters of 4cm, by volume 1:6
Add deodorization liquid, deodorization liquid composition and its most preferably parts by weight are：8 parts of citric acids, 17 parts of extractive of perilla, 0.1 part of sodium chloride,
0.5 part of sodium acid carbonate, 0.001 part of malol, 30 parts of ethanol, 0.04 part of Kaempferitrin and 0.3 part of vitamin E.Immersion, soak time
For 7h.Immersion, soak time is 7h.Smash to pieces, cross 40 mesh sieves, by volume 1:5 add ethyl acetate, stirring, and degreasing time is
24h.Filtering, by volume 1 in filter residue:3 add deionized water, remove grease, and filtering is dried under 45 DEG C of environment, gone
Fat hairtail powder；
2）Enzymolysis：By volume 1 in hairtail powder:5 add distilled water, adjust pH, add pepsin and zymoexciter, stomach
Protease zymoexciter composition and its parts by weight are：0.003 portion of propyl acetate and 0.04 part of vitamin B1.Pepsin it is enzyme-added
It is 2.0 to measure as 3%, pH, and hydrolysis temperature is 36 DEG C, and enzymolysis time is 2.2h.Enzymolysis terminates rear boiling water bath and gone out enzyme 8min, adjusts pH,
Flavor protease and zymoexciter are added, food flavor enzyme zymoexciter composition and its parts by weight are：0.05 part of magnesium acetate and 0.001 part
Dimethyl carbonate.The enzyme concentration of flavor protease is that 3%, pH is 7.0, and hydrolysis temperature is 50 DEG C, and enzymolysis time is 1.7h.Enzymolysis
Boiling water bath goes out enzyme 8min after end, is filtrated to get the thick liquid of high F value oligopeptide；
3）Ultrafiltration：The enzymolysis liquid ultrafiltration that step 2 is obtained, milipore filter specification is 5kDa, obtains filtered solution.High F value oligopeptide is usual
There is 2-9 amino acid residue, mean molecule quantity is less than 1.5kDa, and ultrafiltration can remove macro-molecular protein impurity, and high F value oligopeptide is obtained
To purifying；
4）The de- virtue of activated carbon：Activated carbon is added in filtered solution, activated carbon is that the powdery being made using high-quality cocoanut shell as raw material is lived
Property charcoal, activated carbon adulterates 0.32% nano aluminium oxide and 0.2% nano-sized iron oxide.Stirring, activated carbon and filtered solution solid-to-liquid ratio are 1:
20, pH be 6.0, and temperature is 25 DEG C, and adsorption time is 3h.Centrifugation, oligopeptide powder is obtained by supernatant freeze-drying.Activated carbon into
This is relatively low, and pollution will not be also produced in process of production, easily reclaims.Because the outer telogenesis of granular activated carbon is irregular
Shape, pore structure is more complicated, to free aromatic amino acid adsorption efficiency preferably, and de- virtue rate is higher, while can also play
Good deodorant effect, is effectively improved the quality of high F value oligopeptide；Dopen Nano aluminum oxide and nano-sized iron oxide in activated carbon
Its adsorption rate to free aromatic amino acid can be increased, oligopeptides F values are improved；
5）Gel chromatography：SepHadexG-25 gels are placed in distilled water and soaked, soaking temperature is 60 DEG C, soak time is
6h.Bottle,suction is poured into after being fully swelled, is vacuumized, during which, water is constantly changed and removes and swim in the impurity on surface, sop up gel
Bubble in particle, until supernatant is as clear as crystal, have no any suspension after gel precipitation.Fill post, balance, by oligopeptide powder
It is dissolved in distilled water, oligopeptide powder：Distilled water mass volume ratio is 20mg：0.45 μm of needle cylinder type filter membrane is crossed after 1mL, fully dissolving,
Pressure adsorption loading is taken, treats that sample is just fully flowed into post, switches to elution mode, opened by mobile phase of ultra-pure water
Begin to elute, flow velocity is 0.6 mL/min, 1 is collected per 2min and is managed.Detect often UV absorption of the pipe sample at 220nm and 280nm
Value, sample is merged by peak and collected, freeze-drying.SepHadex G-25 gel particles, have for small peptide and other small molecules
Good separating effect, appearance peak type is stable.Moreover, SepHade gel particles have in weak acid, aqueous slkali and organic solution
There are preferable heat resistance, stability, may be reused after overactivation, also with certain desalination function；
6）HPLC is purified：Using liquid chromatograph, acetonitrile and ultra-pure water are that eluent carries out gradient elution, elution time, elution
Liquid composition and its percentage and flow velocity are followed successively by：0 ~ 5min, acetonitrile 15%, ultra-pure water 85%, flow velocity is 0.5 mL/min；5~
15min, acetonitrile 40%, ultra-pure water 60%, flow velocity is 0.5 mL/min；15 ~ 25min, acetonitrile 80%, ultra-pure water 20%, flow velocity is 0.5
mL/min；25 ~ 30min, acetonitrile 100%, flow velocity is 0.6 mL/min；30 ~ 40min, acetonitrile 40%, ultra-pure water 60%, flow velocity is
0.6 mL/min；40 ~ 50min, acetonitrile 20%, ultra-pure water 80%, flow velocity is 0.6 mL/min.Collect eluent, freeze-drying.Most
The F values of the oligopeptides obtained afterwards can be up to more than 40.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
SEQUENCE LISTING
<110>Zhejiang Ocean university
<120>High F value oligopeptide Coarse liquid separation purification process
<130> 1
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 8
<212> PRT
<213>It is artificial synthesized
<400> 1
Ser Cys Ala Ser Val Cys Lys Ala
1 5
Claims (10)
1. high F value oligopeptide Coarse liquid separation purification process, it is characterised in that following steps：
1）Pretreatment：By hairtail decaptitating, remove the peel, remove internal organ, rinsed well, be cut into small pieces with clear water, soaked and go in deodorization liquid
Raw meat, degreasing；
2）Enzymolysis：Digested using pepsin and flavor protease two-step, be filtrated to get the thick liquid of high F value oligopeptide；
3）Ultrafiltration：The enzymolysis liquid ultrafiltration that step 2 is obtained, obtains filtered solution；
4）The de- virtue of activated carbon：Activated carbon is added in filtered solution, is stirred, supernatant freeze-drying is obtained oligopeptide powder by centrifugation；
5）Gel chromatography：Gel is placed in distilled water and soaked, bottle,suction is poured into after being fully swelled, vacuumizes, post is filled, balanced,
Oligopeptide powder is dissolved in distilled water, loading uses ultrapure water elution, detects absorbance, merge by peak, is freeze-dried；
6）HPLC is purified：Using liquid chromatograph, acetonitrile and ultra-pure water are that eluent carries out gradient elution, collect eluent, cold
It is lyophilized dry.
2. high F value oligopeptide Coarse liquid separation purification process according to claim 1, it is characterised in that：Surpass in described step 3
Filter membrane specification is 4.5 ~ 5.5kDa.
3. high F value oligopeptide Coarse liquid separation purification process according to claim 1, it is characterised in that：It is living in described step 4
Property charcoal be the powdered activated carbon being made using high-quality cocoanut shell as raw material, activated carbon and filtered solution solid-to-liquid ratio are 1:18 ~ 23, pH are
5.5 ~ 6.5, temperature is 22 ~ 26 DEG C, and adsorption time is 2.5 ~ 3.2h.
4. high F value oligopeptide Coarse liquid separation purification process according to claim 1, it is characterised in that：It is living in described step 4
Property charcoal adulterate 0.2 ~ 0.45% nano aluminium oxide and 0.1 ~ 0.3% nano-sized iron oxide.
5. high F value oligopeptide Coarse liquid separation purification process according to claim 1, it is characterised in that：Coagulated in described step 5
Glue uses SepHadex G-25, and soaking temperature is 50 ~ 65 DEG C, and soak time is 5 ~ 6.5h.
6. high F value oligopeptide Coarse liquid separation purification process according to claim 1, it is characterised in that：Fallen in described step 5
Enter bottle,suction, constantly change water during vacuumizing and remove the bubble for swimming in the impurity on surface, sopping up in gel particle, until
Supernatant is as clear as crystal after gel precipitation, have no any suspension.
7. high F value oligopeptide Coarse liquid separation purification process according to claim 1, it is characterised in that：It is few in described step 5
Peptide powder：Distilled water mass volume ratio is 18mg：1mL~22mg：0.42 ~ 0.48 μm of syringe-type filter is crossed after 1mL, fully dissolving
Film.
8. high F value oligopeptide Coarse liquid separation purification process according to claim 1, it is characterised in that：Washed in described step 5
De- liquid is ultra-pure water, and flow velocity is 0.54 ~ 0.65 mL/min, and every 1.8 ~ 2.2min collects 1 and managed.
9. high F value oligopeptide Coarse liquid separation purification process according to claim 1, it is characterised in that：Adopted in described step 6
With gradient elution, elution time, eluent composition and its percentage and flow velocity are followed successively by：0 ~ 5min, acetonitrile 15%, ultra-pure water
85%, flow velocity is 0.45 ~ 0.52 mL/min；5 ~ 15min, acetonitrile 40%, ultra-pure water 60%, flow velocity is 0.45 ~ 0.52 mL/min；
15 ~ 25min, acetonitrile 80%, ultra-pure water 20%, flow velocity is 0.45 ~ 0.52 mL/min；25 ~ 30min, acetonitrile 100%, flow velocity is
0.55~0.62 mL/min；30 ~ 40min, acetonitrile 40%, ultra-pure water 60%, flow velocity is 0.55 ~ 0.62 mL/min；40 ~ 50min,
Acetonitrile 20%, ultra-pure water 80%, flow velocity is 0.55 ~ 0.62 mL/min.
10. high F value oligopeptide Coarse liquid separation purification process according to claim 1, it is characterised in that：Described high F value is few
Contain the high F value oligopeptide that amino acid sequence is SCASVCKA in the thick liquid of peptide.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107586819A (en) * | 2017-09-27 | 2018-01-16 | 舟山达康科技有限公司 | A kind of method that protease hydrolyzed method prepares protein peptides |
| CN108218975A (en) * | 2018-04-02 | 2018-06-29 | 广州赛莱拉干细胞科技股份有限公司 | Oyster Protein high F value oligopeptide with and preparation method thereof |
| CN108517004A (en) * | 2018-04-02 | 2018-09-11 | 广州赛莱拉干细胞科技股份有限公司 | Inkfish high F value oligopeptide with and preparation method thereof |
| CN109824774A (en) * | 2019-03-18 | 2019-05-31 | 时代生物科技（深圳）有限公司 | A method of recycling high activity peptide |
| CN111979286A (en) * | 2020-08-31 | 2020-11-24 | 鲁东大学 | Method for preparing shellfish high F value oligopeptide by combining fermentation method and enzyme method |
| CN113215213A (en) * | 2021-04-27 | 2021-08-06 | 北京养淬堂生物科学技术研究院有限公司 | Preparation process for effectively improving functional activity of small molecule peptide |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104004087A (en) * | 2014-05-12 | 2014-08-27 | 华南理工大学 | High-F-ratio leech oligopeptide, and enzymatic hydrolysis preparation method and application thereof |
| CN105624247A (en) * | 2016-02-29 | 2016-06-01 | 浙江海洋学院 | Preparation method for activator of Nrf2-ARE pathway in tuna high F ratio oligopeptide |
| CN105669853A (en) * | 2016-02-29 | 2016-06-15 | 浙江海洋学院 | Activator for Nrf2-ARE pathway in high fischer ratio oligo-peptide from tuna |
-
2017
- 2017-05-11 CN CN201710329862.2A patent/CN107164440A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104004087A (en) * | 2014-05-12 | 2014-08-27 | 华南理工大学 | High-F-ratio leech oligopeptide, and enzymatic hydrolysis preparation method and application thereof |
| CN105624247A (en) * | 2016-02-29 | 2016-06-01 | 浙江海洋学院 | Preparation method for activator of Nrf2-ARE pathway in tuna high F ratio oligopeptide |
| CN105669853A (en) * | 2016-02-29 | 2016-06-15 | 浙江海洋学院 | Activator for Nrf2-ARE pathway in high fischer ratio oligo-peptide from tuna |
Non-Patent Citations (1)
| Title |
|---|
| 黄程 等: "鲣鱼肉高F值寡肽液的生理活性研究", 《食品工业》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107586819A (en) * | 2017-09-27 | 2018-01-16 | 舟山达康科技有限公司 | A kind of method that protease hydrolyzed method prepares protein peptides |
| CN107586819B (en) * | 2017-09-27 | 2021-06-08 | 舟山市常青海洋食品有限公司 | Method for preparing protein peptide by protease enzymolysis method |
| CN108218975A (en) * | 2018-04-02 | 2018-06-29 | 广州赛莱拉干细胞科技股份有限公司 | Oyster Protein high F value oligopeptide with and preparation method thereof |
| CN108517004A (en) * | 2018-04-02 | 2018-09-11 | 广州赛莱拉干细胞科技股份有限公司 | Inkfish high F value oligopeptide with and preparation method thereof |
| CN109824774A (en) * | 2019-03-18 | 2019-05-31 | 时代生物科技（深圳）有限公司 | A method of recycling high activity peptide |
| CN111979286A (en) * | 2020-08-31 | 2020-11-24 | 鲁东大学 | Method for preparing shellfish high F value oligopeptide by combining fermentation method and enzyme method |
| CN113215213A (en) * | 2021-04-27 | 2021-08-06 | 北京养淬堂生物科学技术研究院有限公司 | Preparation process for effectively improving functional activity of small molecule peptide |
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