CN107389675A - A kind of compound test reagent - Google Patents
A kind of compound test reagent Download PDFInfo
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- CN107389675A CN107389675A CN201710741445.9A CN201710741445A CN107389675A CN 107389675 A CN107389675 A CN 107389675A CN 201710741445 A CN201710741445 A CN 201710741445A CN 107389675 A CN107389675 A CN 107389675A
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- mixed liquor
- dihydrogen phosphate
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- Life Sciences & Earth Sciences (AREA)
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Abstract
The present invention relates to detection reagent field, and in particular to a kind of compound test reagent, comprises the following steps：Prepare the mixed solution of mercuric chloride, magnesium chloride and sodium acetate；The mixed liquor of phosphotungstic acid, sodium dihydrogen phosphate and sulfuric acid is prepared, the pH of mixed liquor is 35；Detection reagent, include the first mixed solution of mercuric chloride, magnesium chloride and sodium acetate；Second mixed liquor of phosphotungstic acid, sodium dihydrogen phosphate and sulfuric acid, the pH of mixed liquor is 35；In use, the volume ratio of the first mixed solution and the second mixed liquor is 1:1‑2:1.Contrast solution is distilled water.Present invention sampling is convenient, simple to operate, report it is rapid and can more early detection cervical carcinogenesis, be a kind of more superior detection method.
Description
Technical field
The present invention relates to detection reagent field, and in particular to a kind of compound test reagent.
Background technology
Existing clinical cervical carcinoma screening and auxiliary examination have following several：1st, cervix Scrape method checks the examination of 2, iodine
Carcinoma 5, excisional cone cervix can not directly be diagnosed by testing 3, uterine neck and cervical canal biopsy 4, vaginoscopy gynecatoptron
Art 6, uterine neck photography 7, fluoroscopy 8, tumor biochemistry diagnosis, above test mode is invasive, it is necessary to provide it is noninvasive and
The high detection reagent of accuracy rate.
The content of the invention
In view of the deficiencies of the prior art, the present invention provides a kind of high sensitivity, the urine mercapto that simple to operate, the degree of accuracy is high
Based compound detection reagent and preparation method.
In order to realize foregoing invention purpose, the present invention uses following technical scheme：A kind of compound test reagent, its feature
It is, comprises the following steps：
（1）Prepare the mixed solution of mercuric chloride, magnesium chloride and sodium acetate；
（2）The mixed liquor of phosphotungstic acid, sodium dihydrogen phosphate and sulfuric acid is prepared, the pH of mixed liquor is 3-5；
（3）In use, step（1）The solution and step of preparation（2）The volume ratio of the solution of preparation is 1:1-2:1；
（4）Contrast solution is distilled water.
Mercuric chloride is in step（1）Mixed solution in concentration be 0.01mol/L, the concentration of magnesium chloride is
0.005mol/L, the mass concentration of sodium acetate is 9wt%.
Step（2）In, the concentration of phosphotungstic acid is 52g/L, and the concentration of sodium dihydrogen phosphate is 9wt%, and the concentration of sulfuric acid is 4-
8wt%。
A kind of urine sulfhydryl compound detection reagent, include the first mixed solution of mercuric chloride, magnesium chloride and sodium acetate；
Second mixed liquor of phosphotungstic acid, sodium dihydrogen phosphate and sulfuric acid, the pH of mixed liquor is 3-5；In use, the first mixed solution and second
The volume ratio of mixed liquor is 1:1-2:1.
Present invention also offers the application detection method of mentioned reagent box：Urina sanguinis sample 1ml is taken to instill contrast solution respectively
And step（1）Mixed solution in, shake up, stand a moment, be separately added into step（2）Solution；Observe contrast solution and test
The color change of solution, if two solution colours unanimously if be determined as feminine gender；If two solution colours are different, and test solution colour and show
The blueness shown is more deeper than control solution colour, is determined as the positive.
The present invention magnesium chloride and sodium acetate are added in mercuric chloride, rise cushioning effect, and do not influence mercuric chloride and
The combination of sulfhydryl compound；Phosphotungstic acid, sodium dihydrogen phosphate and sulfuric acid, it is 3-5 to control pH, improves detection accuracy.
Embodiment
The present invention is further described in detail with reference to specific embodiment.
A kind of compound test reagent, it is characterised in that comprise the following steps：
（1）Prepare the mixed solution of mercuric chloride, magnesium chloride and sodium acetate.
（2）The mixed liquor of phosphotungstic acid, sodium dihydrogen phosphate and sulfuric acid is prepared, the pH of mixed liquor is 4.5.
（3）In use, step（1）The solution and step of preparation（2）The volume ratio of the solution of preparation is 2:1.
（4）Contrast solution is distilled water.
Mercuric chloride is in step（1）Mixed solution in concentration be 0.01mol/L, the concentration of magnesium chloride is
0.005mol/L, the mass concentration of sodium acetate is 9wt%.Step（2）In, the concentration of phosphotungstic acid is 52g/L, sodium dihydrogen phosphate
Concentration is 9wt%, and the concentration of sulfuric acid is 4-8wt%.
Detection method：Urina sanguinis sample 1ml is taken to instill contrast solution 100ml and step respectively（1）Mixed solution 100ml
In, shake up, stand a moment, be separately added into step（2）Solution 50ml；Observe contrast solution and test the color change of solution,
If two solution colours unanimously if be determined as feminine gender；If two solution colours are different, and test the blueness ratio control that solution colour is shown
Solution colour is deeper, is determined as the positive.
Product analysis performance study result is summarized and evaluation：Sensitivity for analysis is（With DL-cysteine densimeter）
0.038mmol/L；Analysis method specificity is 94.6%；It is 96% to determine accuracy；Imprecision in batch（In terms of colour code magnitude）
For 0；Imprecision between batch（In terms of colour code magnitude）For 0.Product stability reaches 2 years.Therefore, Product checking is reproducible,
High specificity, the degree of accuracy is high, and properties of product are stable, can preferably meet the requirement of clinical detection reagent.
Product clinical study results are summarized and evaluation：
1. product passes through the detection for the random urine specimen of Patients with Cervical Cancer made a definite diagnosis 63 maternity ＆ child care centers of province and 135 are good for
The detection of health woman's urine specimen, the results showed that this product is the detection reagent of high specificity, is characterized in simple to operate, without
Special instrument and equipment, patient specimen are left and taken conveniently, and as a result report is quick.
2. the positive rate that this product detects to cancer patient before treatment is 96%, it is to patient's Positive rate in treatment
84.6%；It is positive in Healthy People urine Samples detection, there is 5
3. in detection of this product to same patient, before treatment（Before treatment and in treatment）Positive rate is 96.3%, positive after treatment
Rate is 27%.Show that the effect of this product contributes to patient monitors.
Claims (6)
1. a kind of compound test reagent, it is characterised in that comprise the following steps：
（1）Prepare the mixed solution of mercuric chloride, magnesium chloride and sodium acetate；
（2）The mixed liquor of phosphotungstic acid, sodium dihydrogen phosphate and sulfuric acid is prepared, the pH of mixed liquor is 3-5；
（3）In use, step（1）The solution and step of preparation（2）The volume ratio of the solution of preparation is 1:1-2:1；
（4）Contrast solution is distilled water.
A kind of 2. compound test reagent according to claim 1, it is characterised in that
Mercuric chloride is in step（1）Mixed solution in concentration be 0.01mol/L, the concentration of magnesium chloride is 0.005mol/L,
The mass concentration of sodium acetate is 9wt%.
A kind of 3. compound test reagent according to claim 1, it is characterised in that step（2）In, the concentration of phosphotungstic acid
For 52g/L, the concentration of sodium dihydrogen phosphate is 9wt%, and the concentration of sulfuric acid is 4-8wt%.
4. a kind of urine sulfhydryl compound detection reagent, include the first mixed solution of mercuric chloride, magnesium chloride and sodium acetate；Phosphorus
Second mixed liquor of wolframic acid, sodium dihydrogen phosphate and sulfuric acid, the pH of mixed liquor is 3-5；In use, the first mixed solution and second mixes
The volume ratio for closing liquid is 1:1-2:1.
5. a kind of compound test reagent according to claim 4, it is characterised in that mercuric chloride is in the first mixed solution
In concentration be 0.01mol/L, the concentration of magnesium chloride is 0.005mol/L, and the mass concentration of sodium acetate is 9wt%.
A kind of 6. urine sulfhydryl compound detection reagent according to claim 4, it is characterised in that in the second mixed liquor,
The concentration of phosphotungstic acid is 52g/L, and the concentration of sodium dihydrogen phosphate is 9wt%, and the concentration of sulfuric acid is 4-8wt%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710741445.9A CN107389675A (en) | 2017-08-25 | 2017-08-25 | A kind of compound test reagent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710741445.9A CN107389675A (en) | 2017-08-25 | 2017-08-25 | A kind of compound test reagent |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107389675A true CN107389675A (en) | 2017-11-24 |
Family
ID=60345215
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710741445.9A Withdrawn CN107389675A (en) | 2017-08-25 | 2017-08-25 | A kind of compound test reagent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107389675A (en) |
-
2017
- 2017-08-25 CN CN201710741445.9A patent/CN107389675A/en not_active Withdrawn
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20171124 |
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| WW01 | Invention patent application withdrawn after publication |