CN104714030B - A kind of cervical cancer urine detection test kit - Google Patents
A kind of cervical cancer urine detection test kit Download PDFInfo
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- CN104714030B CN104714030B CN201510097778.3A CN201510097778A CN104714030B CN 104714030 B CN104714030 B CN 104714030B CN 201510097778 A CN201510097778 A CN 201510097778A CN 104714030 B CN104714030 B CN 104714030B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57407—Specifically defined cancers
- G01N33/57411—Specifically defined cancers of cervix
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Abstract
The invention discloses a kind of cervical cancer urine detection test kit, including container 1 and container 2, wherein containing 2ml DTNB solution in container 1, containing 2ml Salkowski's solution in container 2。The invention also discloses the using method of this test kit, take experimenter urina sanguinis 1ml, be first added drop-wise in the container containing DTNB solution, stand 2-5 minute, be subsequently adding in the container containing Salkowski's solution, stand 2-5 minute, observe whether solution has color to change。By chromogenic reaction, described test kit can judge whether experimenter suffers from cervical cancer quickly and easily。
Description
Technical field
The present invention relates to external diagnosis reagent field, specifically, relate to a kind of cervical cancer urine detection test kit and using method thereof。
Background technology
Early discovery is the key for the treatment of tumor, and the development of tumor is divided into five stages: optimum, tumor cell starting period, tumor early stage, tumor mid-term, late period。The fearful part of cancer, in that front three phases, iconography cannot detect at all, even if having arrived tumor mid-term, also only has very professional doctor just can measure with more advanced equipment。The research of World Health Organization (WHO) is thought: the cancer of current 1/3 can be prevented；The cancer of 1/3 can extend patient vitals by active treatment；The cancer of 1/3 can be by early discovery, early diagnosis, early treatment and fully recover。
Cervical cancer is modal gynecologic malignant tumor。The cancer in situ age occurred frequently is 30～35 years old, and infiltrating carcinoma is 45～55 years old, and its morbidity in recent years has the trend of rejuvenation。The method being most commonly used to cervical lesion screening at present is cervical cytological examination, but detecting step very complicated, invasive is high, and charge is higher, makes the examination of cervical cancer be difficult to popularize。Have in bibliographical information cervical cancer patient urine and there is a large amount of non-protein sulfydryl, also report and cervical cancer rapid self-inspection kit (FST-SH) can be used to detect the non-protein sulfydryl in Urine in Patients, thus judging whether patient suffers from the risk (" in detection urine, sulfydryl sieve examines the clinical meaning of cervical cancer and precancerous lesion " of cervical cancer, " Clinical Oncology impurity ", 13 volume o. 11th, 974-978 page by the end of November in 2008)。But this section of document does not disclose particular make-up and the preparation method of FSH-SH。
Chinese patent CN202092960U and CN104181155A discloses the test kit of detection sulfydryl, but these two sections of documents all employ the mercuric chloride (that is, mercuric chloride) with serious health hazards, the health of operator is had certain risk。CN104198404A discloses a kind of cervical carcinoma screening rapid quantitative detection reagent box, wherein use DTNB as developer, but DTNB is the sulfydryl in detection protein, polypeptide, and this method needs to use biochemical instruments to detect, the general family operation of inapplicable patient。
Summary of the invention
It is an object of the invention to provide a kind of detection sensitivity is high, accuracy is good a kind of cervical cancer urine detection test kit and using method thereof, being used for detecting sulfydryl in human urine thus judging whether suffer from cervical cancer。
The cervical cancer urine detection test kit of the present invention, including container 1 and container 2, wherein containing 2mlDTNB solution in container 1, containing 2ml Salkowski's solution in container 2。
Wherein DTNB solution is prepared as follows: first preparation Tris-glycine buffer, containing 1.04gTris(trishydroxymethylaminomethane in every 100ml solution), 0.69g glycine, 0.12gEDTA(ethylenediaminetetraacetic acid), pH=8.0, by 0.4gDTNB(namely, 5,5 '-dithio double; two (2-nitrobenzoic acid)) it is dissolved in the above-mentioned buffer of 100ml, form DTNB solution。
Wherein Salkowski's solution is prepared as follows: first prepare acetate buffer, and 2mol/L sodium acetate solution 98ml adds 2mol/L acetum 2ml mixing, is dissolved in by 4g phosphotungstic acid in the above-mentioned buffer of 100ml, forms Salkowski's solution。
The cervical cancer urine detection test kit of the present invention, its using method is: take experimenter urina sanguinis 1ml, is first added drop-wise in the container containing 2mlDTNB solution, stand 2-5 minute, it is subsequently adding in the container containing 2ml Salkowski's solution, stands 2-5 minute, observe whether solution has color to change。
Criterion: if 1 changes without color, it is judged that for feminine gender, does not have cervical cancer in prompting subject；If 2 colors transfer blueness to, it is judged that for the positive, in prompting subject, there is cervical cancer。
DTNB is that urine is first reacted by the application, the free sulfhydryl groups in conjugated protein for the reagent of free sulfhydryl groups in test protein in Ellman reaction。If experimenter suffers from cervical cancer, then there is a large amount of non-protein sulfhydryl in urine, sulfydryl is reducing agent, phosphotungstic acid can be made to be reduced into tungsten blue and be blueness in sour environment, and then infers that patient suffers from cervical cancer。Therefore, the reagent of the present invention is similarly suitable suffers from albuminuretic patient, improves the scope of application of reagent。Eliminate the interference of protein sulfhydryl simultaneously, also improve the accuracy of test。
Therefore, the invention provides a kind of cervical cancer urine detection test kit and using method thereof, it, by detecting the sulfydryl in Urine in Patients, judges whether patient suffers from cervical cancer。This reagent composition is simple, only needs two kinds of main agents。Easy to operate, patient is in namely operable, simple and fast。And by DTNB Binding proteins sulfydryl, reduce interference, therefore positive rate and accuracy rate are high。
Detailed description of the invention
Describe embodiments of the present invention by the examples below, it will be appreciated by the person skilled in the art that these specific embodiments only indicate that to reach the purpose of the present invention and the enforcement technical scheme that selects, be not the restriction to technical scheme。According to the teachings of the present invention, it is obvious in conjunction with prior art to the improvement of technical solution of the present invention, belongs to the scope of protection of the invention。
A kind of cervical cancer urine detection test kit, including container 1 and container 2, wherein containing 2mlDTNB solution in container 1, containing 2ml Salkowski's solution in container 2。
Wherein DTNB solution is prepared as follows: first preparation Tris-glycine buffer, containing 1.04gTris in every 100ml solution, 0.69g glycine, 0.12gEDTA, pH=8.0, by 0.4gDTNB(namely, 5,5 '-dithio double; two (2-nitrobenzoic acid)) it is dissolved in the above-mentioned buffer of 100ml, form DTNB solution。
Wherein Salkowski's solution is prepared as follows: first prepare acetate buffer, and 2mol/L sodium acetate solution 98ml adds 2mol/L acetum 2ml mixing, is dissolved in by 4g phosphotungstic acid in the above-mentioned buffer of 100ml, forms Salkowski's solution。
The using method of a kind of cervical cancer urine detection test kit: take experimenter urina sanguinis 1ml, first it is added drop-wise in the container containing 2mlDTNB solution, stands 2-5 minute, be subsequently adding in the container containing 2ml Salkowski's solution, stand 2-5 minute, observe whether solution has color to change。
Through the clinical verification of tumour hospital, 320 examples are and detect 298 examples in the Patients with Cervical Cancer that histology makes a definite diagnosis is positive patient, and accuracy rate (coincidence rate) reaches 93.1%。
The cervical cancer urine detection test kit of the present invention makes simple, with low cost, only needs two kinds of main agents DTNB and phosphotungstic acid。And use DTNB to eliminate the protein sulfhydryl interference to testing result, improve the accuracy of testing result。Do not use the reagent with serious health hazards, operator is safer。And without complex instrument, colour developing is substantially, it is easy to observed result。
The foregoing is only the preferred embodiments of the present invention, not the present invention is made any formal and substantial restriction。Those skilled in the art, without departing from the scope of technical solution of the present invention, the equivalent variations of change, modification and the differentiation made when available disclosed above technology contents is the Equivalent embodiments of the present invention；Meanwhile, above example is made by the substantial technological of all foundation present invention the change of any equivalent variations, modification and differentiation etc. are all in the scope being defined by the claims of the present invention。
Claims (1)
1. a cervical cancer urine detection test kit, it is characterized in that, it is made up of container 1 and container 2, wherein containing 2mlDTNB solution in container 1, containing 2ml Salkowski's solution in container 2, wherein DTNB solution is prepared as follows: first preparation Tris-glycine buffer, containing 1.04gTris in every 100ml solution, 0.69g glycine, 0.12gEDTA, pH=8.0, 0.4gDTNB is dissolved in the above-mentioned buffer of 100ml, form DTNB solution, with, wherein Salkowski's solution is prepared as follows: first prepare acetate buffer, 2mol/L sodium acetate solution 98ml adds 2mol/L acetum 2ml mixing, 4g phosphotungstic acid is dissolved in the above-mentioned buffer of 100ml, form Salkowski's solution。
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| CN201510097778.3A CN104714030B (en) | 2015-03-05 | 2015-03-05 | A kind of cervical cancer urine detection test kit |
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| CN201510097778.3A CN104714030B (en) | 2015-03-05 | 2015-03-05 | A kind of cervical cancer urine detection test kit |
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| CN104714030B true CN104714030B (en) | 2016-06-22 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106226295A (en) * | 2016-07-05 | 2016-12-14 | 苏州锐霖生物科技有限公司 | A kind of urine sulfhydryl compound detectable and preparation method |
| CN110006883A (en) * | 2019-04-08 | 2019-07-12 | 深圳华创生物医药科技有限公司 | A kind of urine sulfhydryl compound mercury-free detection reagent and preparation method and application |
| CN110006884A (en) * | 2019-04-08 | 2019-07-12 | 深圳华创生物医药科技有限公司 | A kind of urine sulfhydryl compound mercury-free test strip and preparation method |
| CN110927156A (en) * | 2019-12-26 | 2020-03-27 | 欧迪玛(湖北)生物科技有限公司 | Urine detection kit for cervical cancer and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| GB0103605D0 (en) * | 2001-02-14 | 2001-03-28 | Cairns Hector | Stain |
| CN104198404A (en) * | 2014-08-06 | 2014-12-10 | 福州艾维德生物医药有限公司 | Quick and quantitative detection kit for screening cervical cancer |
| CN104181155A (en) * | 2014-08-10 | 2014-12-03 | 成都煦华生物科技有限公司 | Kit and preparation method thereof |
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