JP2951127B2 - Bacterial vaginosis test - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001] The present invention relates to the detection of bacterial vaginosis by amine detection using a gas phase color reaction which can be visually and objectively tested .
The present invention relates to a novel test method for determining bacterial vaginosis .

[0002]

2. Description of the Related Art In recent years, in the field of obstetrics and gynecology, bacterial vaginosis, in which trichomonas vaginalis and vaginal candidiasis are denied among patients with a chief complaint of vaginal discharge and local inflammation is poor, has been attracting attention. .

[0003] This vaginosis often begins in connection with persistent uterine bleeding, delivery, gynecological surgery, use of endometrial contraceptives and the like.

[0004] Bacterial vaginosis has a subjective symptom of zonation, and cases are overlooked or neglected because it is not accompanied by inflammatory symptoms.

[0005] However, the putrefaction odor gives an unpleasant sensation to a third party, and furthermore, since the course of the disease is chronic, which hinders daily life, it is a very serious problem for patients.

[0006] Oral administration of metonidazole and tinidazole is considered to be effective as a treatment method. There are other topical therapies using chloramphenicol, but the subjective symptoms are improved, but the therapeutic effect is still unclear.

In any case, it is necessary to carry out an examination at an early stage before the progress becomes serious and to give an appropriate treatment.

[0008] Initially, Gadoneira Bajinarishisu is separated from the vaginitis patients to the band under increasing the main complaint in the high frequency from the bottom band by GARDNER, relevance of the vaginitis and Gadoneira Bajinarishisu from the fact that not separated from the healthy person is insist Was.

[0009] Thereafter, Gardnera vaginalis is also present in the vagina of healthy mature women, and other anaerobic bacteria such as Bacteroides and Peptostreptococcus and mycoplasma are present in the vaginal secretion in a considerable number. I understood that.

Recently, Gardnera vaginalis is merely a marker, and it has come to be considered that anaerobic bacteria such as Bacteroides and Peptostreptococcus are causative bacteria.

However, it is thought that not only anaerobic bacteria such as Bacteroides, Peptostreptococcus, and Mobiluncus, but also Gardnera vaginalis and other aerobic bacteria cooperate to cause this pathological condition. Have been.

[0012] These bacterial vaginosis has been diagnosed with an emphasis on the nature examination of vaginal secretions. Vaginal secretions of healthy mature women
Things whereas no abnormality, vaginal secretions of patients vaginal wall from the outside of the cervix in off-white cream, with cracks covered in a thin film form to the vaginal opening, dropping the small amount 10 to 20% KOH rancidity Is increased.

[0013] As a diagnostic standard for bacterial vaginosis, under the gray belt,
Vaginal pH 5 or higher, detection of amine odor, Cle C
test for detection of ell (more than 20% of endothelial cells)
Three out of four positives are diagnosed as bacterial vaginosis.

[0014]

In order to rapidly examine bacterial vaginosis, the above-mentioned test for confirming the putrefaction odor of vaginal secretions is considered to be effective for daily inspection.

This involves collecting a small amount of vaginal secretion and
This is an amine odor test for smelling putrefaction odor which is enhanced by dropping% KOH.

Naturally, the detection of amine odor varies from individual to individual, and the odor sensation varies depending on the physical condition, and the odor sensation is paralyzed as a large number of samples are processed, making it extremely difficult to obtain reproducible data. Therefore, the reliability is poor.

The present invention has been made in view of the above-mentioned problems in the prior art, and is not limited to amine odors having poor reliability. Detection of bacterial vaginosis
It is intended to provide a novel test method for bacterial vaginosis .

[0018]

In order to solve the above problems SUMMARY OF THE INVENTION In bacterial vaginosis tests of the present invention, the after evaporation by adding an alkali solution to vaginal secretions trial <br/> fees the amine <br/> sample especially to detect by vapor phase color reaction
To determine the presence or absence of bacterial vaginosis .

The gas phase color reaction is a color reaction with an amine reaction color substance such as quinhydrone and / or a pH indicating reaction substance.

Furthermore, speaking in detail, the present invention provides an aseptically vaginal secretions collected by vaginal washing placed in a vessel such as a test tube with sterile saline, was added an alkaline solution, quinhydrone, etc. Solid phase containing amine-reactive coloring substance (filter paper, etc.)
And / or pH test reaction the solid phase (pH test paper) to thereby respectively encapsulated suspended vertical is intended to confirm the amines from the color change.

The solid containing the amine reactive coloring substance such as quinhydrone phase (filter paper), or pH test reaction solid phase (pH test paper) may be singly suspended in the reaction vessel vertical but is a combination of both In this case, the amine detection accuracy is improved.

The detection amines include primary amines, secondary amines, tertiary amines, and rarely, quaternary amines.

The color tone of the reaction with the filter paper containing quinhydrone is as follows: primary amine is purple, secondary amine is red,
Tertiary amines develop yellow-orange color, and rarely quaternary amines develop green-yellow color.

As the amine-reactive coloring substance, any substance can be used as long as it reacts with amines to give a color, but quinhydrone is preferred.

Thymol blue, cresol red and the like are used as a pH indicating reactant. The pH indicating reactant is not particularly limited as long as it can capture the alkalinity of amines.

[0026] The case of using a thymol blue, turned yellow by the presence of amines, color change occurs in purple from yellow in the cresol red.

The present invention is not limited to vaginal secretions of bacterial vaginosis, but can be applied to any sample that generates amines.

[0028]

EXAMPLES The present invention will be described more specifically with reference to the following examples.

Example 1 A small amount of vaginal secretion collected by aseptically washing the vagina with sterile physiological saline was placed in a 10 cm test tube, and 0.5 ml (mL ) of 5N (mol / L) NaOH saturated with sodium chloride was added. )
After addition, to confirm the amines from the color change by the respective encapsulated suspension vertical filter paper and thymol blue pH test paper containing immediate 1% quinhydrone. The results are shown in Table 1.

Comparative Example (Conventional Method) A small amount of vaginal secretion obtained by aseptically washing the vagina with sterile physiological saline was placed on a small slide, and 10% KOH was dropped on the slide to smell the amine odor. The results are shown in Table 1.

[0031]

[Table 1]

Note: The evaluation in Table 1 is as follows. Amine odor: ++ strongly Yes + Yes - No quinhydrones reaction: ++ strongly positive (strong change from yellow to red) + positive (change from yellow to red) - negative (yellow) thymol blue reaction: ++ strongly positive (yellow from blue strength change) + positive in color (change to yellow color from blue color) - negative (blue color)

From the results in Table 1, it can be seen that bacterial vaginosis according to the present invention was obtained .
The inspection method has a good correlation with the conventional method (amine odor),
It was confirmed that the operation was simple.

[0034]

As described above, according to the present invention, a gas-phase color reaction that can be visually and objectively inspected is used instead of an unreliable amine odor that must rely on olfaction. There line detection of amines, thereby bacterial
Judgment of the presence or absence of vaginosis enables quick and simple mass testing.

Continuation of the front page (58) Field surveyed (Int.Cl. ⁶ , DB name) G01N 33/48-33/52 G01N 33/58-33/98 G01N 31/00-31/22 CA (STN)

Claims (4)

(57) [Claims] 1. A determination whether the bacterial vaginosis by amines in the sample after evaporation of by adding an alkali solution to vaginal secretions sample is detected by vapor phase color reaction
Bacterial vaginosis test method characterized by performing . 2. The method according to claim 1, wherein the gas phase color reaction is a color reaction with an amine reaction color substance and / or a pH indicating reaction substance. 3. Vaginal washing with sterile physiological saline aseptically
After adding an alkaline solution to the collected vaginal secretions,
And / or a solid phase containing a pH test reaction
, Respectively, and suspend them, and remove amines from the color tone change.
Detection to determine the presence or absence of bacterial vaginosis
Characteristic bacterial vaginosis test method . 4. The method of claim 2 or 3 The method according to wherein the amine reactive coloring substance is quinhydrone.