CN107384585A - A kind of grading system for antarctic krill oil method - Google Patents
A kind of grading system for antarctic krill oil method Download PDFInfo
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- CN107384585A CN107384585A CN201710857695.9A CN201710857695A CN107384585A CN 107384585 A CN107384585 A CN 107384585A CN 201710857695 A CN201710857695 A CN 201710857695A CN 107384585 A CN107384585 A CN 107384585A
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- krill oil
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- euphausia superba
- antarctic krill
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
Abstract
A kind of method the invention provides grading system for antarctic krill oil, including, first order extraction is carried out to euphausia superba powder using acetone, obtains one-level extraction antarctic krill oil and one-level extraction euphausia superba powder；Second level extraction is carried out to one-level extraction euphausia superba powder using alkanes solvent, obtains two level extraction antarctic krill oil and two level extraction euphausia superba powder；Third level extraction is carried out to two level extraction euphausia superba powder using alcohols solvent, obtains three-level extraction antarctic krill oil.We invents carries out three step extractions, the classification not only to be become more meticulous to lipid, while the also lipid in the extraction shrimp med of maximizing to krill meal using the solvent of three kinds of opposed polarities.
Description
Technical field
The invention belongs to marine organisms field of deep, and in particular to a kind of grading system for antarctic krill oil method.
Background technology
Antarctic krill (Euphausia superba), commonly referred to as krill, it is one kind weight for living in Southern Oceans
Marine organisms are wanted, its stock number is about 3.79 hundred million tons.The fresh shrimp of krill is about 11.9~15.4% containing albumen, and lipid 0.5~
3.6%, ash content 3%, carbohydrate 2%, remaining is moisture.Antarctic krill oil is referred to as by the lipid extracted in krill, in recent years,
Antarctic krill oil is of increasing concern because of its unique 26S Proteasome Structure and Function.
The main component of common edible oil is sweet three ester (95%), and the composition of antarctic krill oil is then complex.Research
Report, it is 22.1-38.4% that sweet three ester in krill lipid, which only contains, and polar lipid content is up to 56.4-72.0%.Pole
Property lipid is mainly phosphatide, DAG and a sweet ester etc..The krill algae that ingests is made a living, therefore EPA (10- are rich in oil
20%) presence is combined and DHA (8-18%), and with phosphatide.In addition, krill oil is also containing abundant micro constitutent, such as shrimp
Blue or green element, tocopherol, vitamin A, sterol etc..
Extractive technique at present, the extensive extraction mostly " finished off with one blow ", but the extensive extractive technique of this " finishing off with one blow " is only
The extraction efficiency of lipid has been paid close attention to, and the composition of extracted krill oil is limited to krill raw material, it is difficult to it is consistent, especially
Larger difference be present in phosphatide and micro constitutent content in shrimp sauce.Therefore, a kind of new finer extractive technique is needed badly, it is right
Krill lipid carries out grading extraction, prepares different content of phospholipid and the krill oil of micro constitutent, can both enrich the species of krill oil,
The krill oil of difference in functionality characteristic is prepared, the krill oil of target proportion of composing can be mixed as needed again, constant shrimp sauce is whole
Product quality.
The content of the invention
The purpose of this part is to summarize some aspects of embodiments of the invention and briefly introduce some preferably to implement
Example.It may do a little simplified or be omitted to avoid making our department in this part and the description of the present application summary and denomination of invention
Point, the purpose of specification digest and denomination of invention obscure, and this simplification or omit and cannot be used for limiting the scope of the present invention.
In view of technological gap of the above-mentioned and/or existing grading system for the method for antarctic krill oil, it is proposed that the present invention.
Therefore, a kind of method it is an object of the present invention to provide grading system for antarctic krill oil.
In order to solve the above technical problems, the invention provides following technical scheme：A kind of grading system is for antarctic krill oil
Method, including, first order extraction is carried out to euphausia superba powder using acetone, obtains one-level extraction antarctic krill oil and one-level extraction
Euphausia superba powder；Second level extraction is carried out to one-level extraction euphausia superba powder using alkanes solvent, obtains two level extraction south
Pole krill oil and two level extraction euphausia superba powder；The third level is carried out using alcohols solvent to two level extraction euphausia superba powder to carry
Take, obtain three-level extraction antarctic krill oil.
A kind of preferred scheme as grading system of the present invention for the method for antarctic krill oil, wherein：It is described to use third
Ketone carries out first order extraction to euphausia superba powder, and it is by mass volume ratio 1 by euphausia superba powder and acetone：1~10 mixing is equal
Even, stirring reaction, stirring is filtered immediately after terminating, and one-level extraction antarctic krill oil is obtained after filtrate desolvation, filter residue is through true
Sky obtains one-level extraction euphausia superba powder after drying.
A kind of preferred scheme as grading system of the present invention for the method for antarctic krill oil, wherein：The acetone, its
Temperature is -10~10 DEG C.
A kind of preferred scheme as grading system of the present invention for the method for antarctic krill oil, wherein：The stirring is anti-
Should, it is to stir 5~40min in -10~10 DEG C, 100~1000rpm.
A kind of preferred scheme as grading system of the present invention for the method for antarctic krill oil, wherein：It is described to use alkane
Class solvent carries out second level extraction to one-level extraction euphausia superba powder, and it is that the one-level is extracted into euphausia superba powder and alkane
Class solvent is well mixed, and stirs 5~40min in 20~40 DEG C, 100~1000rpm, stirring is filtered immediately after terminating, and filtrate takes off
Except two level extraction antarctic krill oil is obtained after solvent, two level extraction euphausia superba powder is obtained after filter residue is vacuum dried.
A kind of preferred scheme as grading system of the present invention for the method for antarctic krill oil, wherein：Described in the general
One-level extraction euphausia superba powder is well mixed with alkanes solvent, its be by the one-level extract euphausia superba powder and alkanes solvent by
Mass volume ratio 1：1~10 is well mixed.
A kind of preferred scheme as grading system of the present invention for the method for antarctic krill oil, wherein：The alkanes is molten
Agent includes n-hexane or isohexane.
A kind of preferred scheme as grading system of the present invention for the method for antarctic krill oil, wherein：It is described to use alcohol
Class solvent carries out third level extraction to two level extraction euphausia superba powder, and it is that the two level is extracted into euphausia superba powder and alcohol
Class solvent is well mixed, and stirs 5~40min in 30~50 DEG C, 100~1000rpm, stirring is filtered immediately after terminating, and filtrate takes off
Antarctic krill oil is extracted except three-level is obtained after solvent.
A kind of preferred scheme as grading system of the present invention for the method for antarctic krill oil, wherein：Described in the general
Two level extraction euphausia superba powder is well mixed with alcohols solvent, its be by the two level extract euphausia superba powder and alcohols solvent by
Mass volume ratio 1：1~10 is well mixed.
A kind of preferred scheme as grading system of the present invention for the method for antarctic krill oil, wherein：The alcohols is molten
Agent includes ethanol or isopropanol.
Beneficial effect possessed by the present invention：
(1) present invention carries out three step extractions using the solvent of three kinds of opposed polarities to krill meal, not only carries out essence to lipid
The classification of refinement, while the also lipid in the extraction shrimp med of maximizing, and obtained antarctic krill oil has outstanding resist
Oxidisability.
(2) three kinds of shrimp sauces prepared by the present invention are utilized, there is unique component characteristic, are advantageous to develop difference in functionality
Shrimp sauce product.
(3) three kinds of shrimp sauces produced by the present invention are utilized, different allotments can be carried out as requested, are advantageous to constant krill production
Product form, and meet different consumption demands.
Brief description of the drawings
In order to illustrate the technical solution of the embodiments of the present invention more clearly, required use in being described below to embodiment
Accompanying drawing be briefly described, it should be apparent that, drawings in the following description are only some embodiments of the present invention, for this
For the those of ordinary skill of field, without having to pay creative labor, it can also be obtained according to these accompanying drawings other
Accompanying drawing.Wherein：
Fig. 1 is that DPPH removes free radical experimental result picture in embodiment 7.
Embodiment
In order to facilitate the understanding of the purposes, features and advantages of the present invention, with reference to specific embodiment pair
The embodiment of the present invention is described in detail.
Many details are elaborated in the following description to facilitate a thorough understanding of the present invention, still the present invention can be with
It is different from other manner described here using other to implement, those skilled in the art can be without prejudice to intension of the present invention
In the case of do similar popularization, therefore the present invention is not limited by following public specific embodiment.
Secondly, " one embodiment " or " embodiment " referred to herein refers to may be included at least one realization side of the present invention
Special characteristic, structure or characteristic in formula." in one embodiment " that different places occur in this manual not refers both to
Same embodiment, nor the single or selective embodiment mutually exclusive with other embodiment.
Embodiment 1
(1) 100g euphausia superba powders, first order extraction are taken：By krill meal and acetone (4 DEG C of precooling 6h) according to 1:2(w/v)
Mixing, 15min, mixing speed 300r/min are stirred at 4 DEG C, stir and filtered on buchner funnel is used after terminating, filtrate precipitation,
The antarctic krill oil of one-level extraction is obtained after drying, the oily yield is calculated after weighing and determines chemical composition.After one-level is extracted
The euphausia superba powder filtered out under conditions of -0.1Mpa after lower dry precipitation, is weighed standby at 60 DEG C；
(2) second level is extracted：The shrimp med that will be obtained after (1) is extracted is with n-hexane according to 1:2 (w/v) are mixed, at 30 DEG C
Lower stirring 15min, mixing speed 300r/min, stirring use filtered on buchner funnel, filtrate precipitation, obtain two after drying after terminating
The antarctic krill oil of level extraction, calculates the oily yield and determines chemical composition after weighing.The South Pole filtered out after two level is extracted
Krill meal after drying precipitation under conditions of -0.1Mpa, is weighed standby at 60 DEG C；
(3) third level is extracted：The shrimp med that will be obtained after (2) are extracted is with ethanol according to 1:3 (w/v) are mixed, at 40 DEG C
15min, mixing speed 300r/min are stirred, stirring uses filtered on buchner funnel, filtrate precipitation, obtains three-level after drying after terminating
The antarctic krill oil of extraction, the oily yield is calculated after weighing and determines chemical composition.
The grading system of embodiment 1 accounts for the percentage of total extract component for the yield of gained krill oil, main composition and each composition
It is as shown in the table.
| One-level krill oil | Two level krill oil | Three-level krill oil | |
| Oil extracting rate (% shrimp meds) | 5.08 | 4.80 | 9.11 |
| Phosphatide (g/100g shrimp sauces) | 2.32 | 39.00 | 59.52 |
| (g100g shrimp meds) | 0.12 | 1.88 | 5.42 |
| (% always extracts phosphatide) | 1.62 | 25.34 | 73.05 |
| Astaxanthin (mg/kg shrimp sauces) | 505.00 | 34.92 | 7.48 |
| (mg/kg shrimp meds) | 25.56 | 1.66 | 0.68 |
| (% always extracts astaxanthin) | 91.61 | 5.95 | 2.44 |
| Tocopherol (mg/100g shrimp sauces) | 29.39 | 12.98 | 3.65 |
| (mg/100g shrimp meds) | 1.48 | 0.62 | 0.33 |
| (% always extracts tocopherol) | 60.91 | 25.51 | 13.58 |
| Vitamin A (mg/100g shrimp sauces) | 34.32 | 12.33 | 2.27 |
| (mg/100g shrimp meds) | 1.74 | 0.59 | 0.21 |
| (% always extracts vitamin A) | 68.50 | 23.23 | 8.27 |
| Sterol (mg/g shrimp sauces) | 27.95 | 17.72 | 9.97 |
| (mg/g shrimp meds) | 1.42 | 0.85 | 0.91 |
| (% always extracts sterol) | 44.65 | 26.73 | 28.62 |
Embodiment 2
(1) 200g euphausia superba powders, first order extraction are taken：By krill meal and acetone (10 DEG C of precooling 6h) according to 1:3(w/v)
Mixing, 10min, mixing speed 200r/min are stirred at 10 DEG C, stirring uses filtered on buchner funnel after terminating, and filtrate takes off
The antarctic krill oil of one-level extraction is obtained after molten, dry, the oily yield is calculated after weighing and determines chemical composition.Extracted by one-level
The euphausia superba powder filtered out afterwards at 60 DEG C, under -0.1Mpa under conditions of dry precipitation after, weigh standby；
(2) second level is extracted：The shrimp med that will be obtained after (1) is extracted is with isohexane according to 1:3 (w/v) are mixed, at 30 DEG C
Lower stirring 15min, mixing speed 300r/min, stirring use filtered on buchner funnel, filtrate precipitation, obtain two after drying after terminating
The antarctic krill oil of level extraction, calculates the oily yield and determines chemical composition after weighing.The South Pole filtered out after two level is extracted
Krill meal at 60 DEG C, under -0.1Mpa under conditions of dry precipitation after, weigh standby；
(3) third level is extracted：The shrimp med that will be obtained after (2) are extracted is with ethanol according to 1:4 (w/v) are mixed, at 35 DEG C
25min, mixing speed 300r/min are stirred, stirring uses filtered on buchner funnel, filtrate precipitation, obtains three-level after drying after terminating
The antarctic krill oil of extraction, the oily yield is calculated after weighing and determines chemical composition.
The grading system of embodiment 2 accounts for the percentage of total extract component for the yield of gained krill oil, main composition and each composition
It is as shown in the table.
Embodiment 3
(1) 250g euphausia superba powders, first order extraction are taken：By krill meal and acetone (0 DEG C of precooling 6h) according to 1:3(w/v)
Mixing, 20min, mixing speed 100r/min are stirred at 0 DEG C, stir and filtered on buchner funnel is used after terminating, filtrate precipitation,
The antarctic krill oil of one-level extraction is obtained after drying, the oily yield is calculated after weighing and determines chemical composition.After one-level is extracted
The euphausia superba powder filtered out at 60 DEG C, under -0.1Mpa under conditions of dry precipitation after, weigh standby；
(2) second level is extracted：The shrimp med that will be obtained after (1) is extracted is with n-hexane according to 1:3 (w/v) are mixed, at 25 DEG C
Lower stirring 20min, mixing speed 200r/min, stirring use filtered on buchner funnel, filtrate precipitation, obtain two after drying after terminating
The antarctic krill oil of level extraction, calculates the oily yield and determines chemical composition after weighing.The South Pole filtered out after two level is extracted
Krill meal at 60 DEG C, under -0.1Mpa under conditions of dry precipitation after, weigh standby；
(3) third level is extracted：The shrimp med that will be obtained after (2) are extracted is with isopropanol according to 1:4 (w/v) are mixed, at 45 DEG C
Lower stirring 20min, mixing speed 200r/min, stirring use filtered on buchner funnel, filtrate precipitation, obtain three after drying after terminating
The antarctic krill oil of level extraction, calculates the oily yield and determines chemical composition after weighing.
The grading system of embodiment 3 accounts for the percentage of total extract component for the yield of gained krill oil, main composition and each composition
It is as shown in the table.
| One-level krill oil | Two level krill oil | Three-level krill oil | |
| Oil extracting rate (% shrimp meds) | 5.69 | 6.74 | 9.77 |
| Phosphatide (g/100g shrimp sauces) | 3.18 | 31.24 | 61.43 |
| (g100g shrimp meds | 0.18 | 2.11 | 6.00 |
| (% always extracts phosphatide) | 2.18 | 25.40 | 72.41 |
| Astaxanthin (mg/kg shrimp sauces) | 568.10 | 26.74 | 5.13 |
| (mg/kg shrimp meds) | 32.32 | 1.80 | 0.50 |
| (% always extracts astaxanthin) | 93.35 | 5.20 | 1.45 |
| Tocopherol (mg/100g shrimp sauces) | 35.13 | 10.63 | 4.92 |
| (mg/100g shrimp meds) | 2.00 | 0.72 | 0.48 |
| (% always extracts tocopherol) | 62.54 | 22.42 | 15.04 |
| Vitamin A (mg/100g shrimp sauces) | 40.14 | 11.36 | 1.67 |
| (mg/100g shrimp meds) | 2.28 | 0.77 | 0.16 |
Embodiment 4
To obtain the krill oil product of content of phospholipid 40%, different proportion is carried out to three kinds of krill oils in embodiment 1
Allotment：One-level extracts krill oil 34%, three-level extraction krill oil 66%；Or one-level extraction krill oil 22%, two level extraction krill
Oil 35%, three-level extraction krill oil 43%.
Embodiment 5
To obtain content of phospholipid 35%, content astaxanthin 200ppm krill oil product, to three kinds of krills in embodiment 1
Oil carries out the allotment of different proportion：One-level extracts krill oil 38%, two level extraction krill oil 14%, three-level extraction krill oil
48%.
Embodiment 6
To obtain content of phospholipid 30%, tocopherol content 18mg/100g krill oil product, to three kinds in embodiment 2
Shrimp sauce carries out the allotment of different proportion：One-level extracts krill oil 40%, two level extraction krill oil 20%, three-level extraction krill oil
40%.
Embodiment 7
Free radical experiment is removed using DPPH, by the way that the DPPH of shrimp sauce radicals scavenging inhibiting rate and standard items is water-soluble
Property tocopherol (Trolox) radicals scavenging inhibiting rate compare, with Trolox Equivalent Antioxidant Capacities evaluate three kinds of shrimp sauces body
Outer oxidation resistance.As a result prove that three kinds of shrimp sauces have significant difference at anti-oxidant aspect.
Experimental method：Each 0.5g of shrimp sauce of Example 1, is dissolved in 100ml ethyl acetate, by solution 2mL and concentration
It is well mixed for 0.15mmoL/L DPPH solution 2mL, dark place reaction 2h, the determination sample absorbance under 517nm wavelength.First root
Free radical scavenging activity is calculated according to absorbance, then according to inhibiting rate
The computational methods of inhibiting rate are：K%=[1- (A1-A2)/A3] × 100%
Wherein, A1：The absorbance of 2mLDPPH solution+2mL product to be tested solution
A2：The absorbance of 2mL product to be tested solution+2mL solvents,
A3：The absorbance of 2mL DPPH solution+2mL solvents.
Meanwhile using Trolox as standard items, the standard curve of the Trolox free radical scavenging activities under various concentrations is drawn, will
The free radical scavenging activity of shrimp sauce contrasts with standard curve, calculates the Trolox Equivalent Antioxidant Capacities of shrimp sauce.
As a result experimental result shows that three kinds of shrimp sauces are respectively provided with powerful oxidation resistance as shown in Figure of description Fig. 1,
And the oxidation resistance for extracting shrimp sauce with three-level especially is most strong.
As can be seen here, the present invention carries out three step extractions using the solvent of three kinds of opposed polarities to krill meal, not only to lipid
The classification to be become more meticulous, at the same also maximizing extraction shrimp med in lipid, and obtained antarctic krill oil have it is excellent
Elegant inoxidizability；Using three kinds of shrimp sauces prepared by the present invention, there is unique component characteristic, be advantageous to develop difference in functionality
Shrimp sauce product；Using three kinds of shrimp sauces produced by the present invention, different allotments can be carried out as requested, be advantageous to constant krill production
Product form, and meet different consumption demands.
It should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention and it is unrestricted, although with reference to preferable
The present invention is described in detail embodiment, it will be understood by those within the art that, can be to the technology of the present invention
Scheme is modified or equivalent substitution, and without departing from the spirit and scope of technical solution of the present invention, it all should cover in this hair
Among bright right.
Claims (10)
1. a kind of grading system is for the method for antarctic krill oil, it is characterised in that：Including,
First order extraction is carried out to euphausia superba powder using acetone, obtains one-level extraction antarctic krill oil and one-level extraction South Pole phosphorus
Shrimp med；
Second level extraction is carried out to one-level extraction euphausia superba powder using alkanes solvent, obtains two level extraction antarctic krill oil
Euphausia superba powder is extracted with two level；
Third level extraction is carried out to two level extraction euphausia superba powder using alcohols solvent, obtains three-level extraction krill
Oil.
2. according to claim 1 grading system for antarctic krill oil method, it is characterised in that：It is described to use acetone to the South Pole
Krill meal carries out first order extraction, and it is by mass volume ratio 1 by euphausia superba powder and acetone：1~10 is well mixed, and stirring is anti-
Should, stirring is filtered immediately after terminating, and one-level extraction antarctic krill oil is obtained after filtrate desolvation, after filter residue is vacuum dried
Euphausia superba powder is extracted to one-level.
3. according to claim 2 grading system for antarctic krill oil method, it is characterised in that：The acetone, its temperature for-
10~10 DEG C.
4. according to Claims 2 or 3 grading system for antarctic krill oil method, it is characterised in that：The stirring reaction, its
It is to stir 5~40min in -10~10 DEG C, 100~1000rpm.
5. according to claim 1 grading system for antarctic krill oil method, it is characterised in that：It is described to use alkanes solvent pair
The one-level extraction euphausia superba powder carries out second level extraction, and it is to mix one-level extraction euphausia superba powder with alkanes solvent
Close uniformly, stir 5~40min in 20~40 DEG C, 100~1000rpm, stirring is filtered immediately after terminating, after filtrate desolvation
Two level extraction antarctic krill oil is obtained, two level extraction euphausia superba powder is obtained after filter residue is vacuum dried.
6. according to claim 5 grading system for antarctic krill oil method, it is characterised in that：It is described to extract the one-level
Euphausia superba powder is well mixed with alkanes solvent, and it is by quality volume by one-level extraction euphausia superba powder with alkanes solvent
Than 1：1~10 is well mixed.
7. according to the grading system of claim 5 or 6 for the method for antarctic krill oil, it is characterised in that：The alkanes solvent bag
Include n-hexane or isohexane.
8. according to claim 1 grading system for antarctic krill oil method, it is characterised in that：It is described to use alcohols solvent pair
The two level extraction euphausia superba powder carries out third level extraction, and it is to mix two level extraction euphausia superba powder with alcohols solvent
Close uniformly, stir 5~40min in 30~50 DEG C, 100~1000rpm, stirring is filtered immediately after terminating, after filtrate desolvation
Obtain three-level extraction antarctic krill oil.
9. according to claim 8 grading system for antarctic krill oil method, it is characterised in that：It is described to extract the two level
Euphausia superba powder is well mixed with alcohols solvent, and it is by quality volume by two level extraction euphausia superba powder with alcohols solvent
Than 1：1~10 is well mixed.
10. according to claim 8 or claim 9 grading system for antarctic krill oil method, it is characterised in that：The alcohols solvent bag
Include ethanol or isopropanol.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108998231A (en) * | 2018-08-06 | 2018-12-14 | 山东鲁华海洋生物科技有限公司 | A kind of method that two-step method prepares high content astaxanthin antarctic krill oil |
| CN109097190A (en) * | 2018-08-31 | 2018-12-28 | 山东鲁华海洋生物科技有限公司 | A method of it being enriched with phosphatide from krill oil |
| CN110643421A (en) * | 2019-09-03 | 2020-01-03 | 武汉轻工大学 | Preparation method for producing three kinds of microalgae oil by three-step solvent method |
| CN112940848A (en) * | 2019-12-11 | 2021-06-11 | 国投生物科技投资有限公司 | EPA-rich microalgae grease with different polarities and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102925284A (en) * | 2012-10-25 | 2013-02-13 | 山东师范大学 | Technique for extracting low-cholesterol-content krill oil from Antarctic krill |
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2017
- 2017-09-21 CN CN201710857695.9A patent/CN107384585B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102925284A (en) * | 2012-10-25 | 2013-02-13 | 山东师范大学 | Technique for extracting low-cholesterol-content krill oil from Antarctic krill |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108998231A (en) * | 2018-08-06 | 2018-12-14 | 山东鲁华海洋生物科技有限公司 | A kind of method that two-step method prepares high content astaxanthin antarctic krill oil |
| CN109097190A (en) * | 2018-08-31 | 2018-12-28 | 山东鲁华海洋生物科技有限公司 | A method of it being enriched with phosphatide from krill oil |
| CN110643421A (en) * | 2019-09-03 | 2020-01-03 | 武汉轻工大学 | Preparation method for producing three kinds of microalgae oil by three-step solvent method |
| CN112940848A (en) * | 2019-12-11 | 2021-06-11 | 国投生物科技投资有限公司 | EPA-rich microalgae grease with different polarities and preparation method thereof |
| CN112940848B (en) * | 2019-12-11 | 2023-11-21 | 国投生物科技投资有限公司 | EPA-enriched microalgae grease with different polarities and preparation method thereof |
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| CN107384585B (en) | 2020-10-23 |
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