CN107233260B - Compound tea polyphenol nanoemulsion and preparation method and application thereof - Google Patents

Compound tea polyphenol nanoemulsion and preparation method and application thereof Download PDF

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CN107233260B
CN107233260B CN201710565211.3A CN201710565211A CN107233260B CN 107233260 B CN107233260 B CN 107233260B CN 201710565211 A CN201710565211 A CN 201710565211A CN 107233260 B CN107233260 B CN 107233260B
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nanoemulsion
tea polyphenol
percent
polyphenol
oil
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CN107233260A (en
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袁莉
高春霞
李建科
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Jingyang Manzifu Tea Co ltd
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Shaanxi Normal University
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463—Organic compounds; Microorganisms; Enzymes
    • A23L3/3472—Compounds of undetermined constitution obtained from animals or plants
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463—Organic compounds; Microorganisms; Enzymes
    • A23L3/3481—Organic compounds containing oxygen
    • A23L3/349—Organic compounds containing oxygen with singly-bound oxygen
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463—Organic compounds; Microorganisms; Enzymes
    • A23L3/3481—Organic compounds containing oxygen
    • A23L3/3508—Organic compounds containing oxygen containing carboxyl groups
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
    • A61K8/04—Dispersions; Emulsions
    • A61K8/06—Emulsions
    • A61K8/068—Microemulsions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
    • A61K8/4973—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
    • A61K8/498—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom having 6-membered rings or their condensed derivatives, e.g. coumarin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/52—Stabilizers
    • A61K2800/522—Antioxidants; Radical scavengers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/90—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in food processing or handling, e.g. food conservation

Abstract

The invention relates to a compound tea polyphenol nanoemulsion and a preparation method and application thereof, wherein the compound tea polyphenol nanoemulsion has stable property and particle size less than 100nm, belongs to W/O type tea polyphenol nanoemulsion, and comprises the following raw materials in percentage by mass: the W/O nano-emulsion is simple in preparation method, small in emulsion droplet particles, uniform in distribution, small in viscosity and good in fluidity, can improve the stability of tea polyphenol in food and pharmaceutical preparations, and expands the application range of the tea polyphenol.

Description

Compound tea polyphenol nanoemulsion and preparation method and application thereof
Technical Field
The invention belongs to the technical field of research of tea polyphenol nanoemulsion materials, and particularly relates to a compound tea polyphenol nanoemulsion as well as a preparation method and application thereof.
Background
The tea polyphenol is a general term of all polyhydroxy phenolic compounds in tea, is a main effective component of the tea and accounts for 20-30% of the dry weight of the tea. Tea polyphenol has various physiological functions and efficacies of scavenging free radicals, resisting aging, resisting tumors, reducing blood fat, inhibiting bacteria, beautifying and the like, but the tea polyphenol is water-soluble, has poor and unstable fat solubility and low bioavailability, is easy to oxidize, and limits the application of the tea polyphenol in industries such as food, medicine and the like, wherein the physiological efficacies of the tea polyphenol can not be fully exerted.
Nanoemulsions, also known as microemulsions. The colloidal dispersion system consists of an oil phase, a water phase, a surfactant and absolute ethyl alcohol, has the particle size of 10-100nm, is spherical in most liquid drops, uniform in size, transparent or semitransparent, stable in thermodynamics, isotropic, stable in hot-pressing sterilization and high-speed centrifugation, and is free of layering. The nanoemulsion is used as a novel carrier, has the advantages of good stability and permeability, low viscosity, easiness in preparation and storage and the like, can improve the stability, dispersibility and solubility of a tested substance, plays a role in slow release and targeting, is widely applied to the fields of food, medicine, beauty treatment, chemical industry, agriculture and the like, and can be divided into Water-in-Oil (W/O) and Oil-in-Water (O/W). Wherein, the W/O type nano-emulsion can increase the fat solubility and the stability of the water-soluble coating substance.
Therefore, the invention firstly provides the concept of the tea polyphenol nanoemulsion and further develops the W/O type compound tea polyphenol nanoemulsion in order to improve the oxidation resistance.
Disclosure of Invention
Aiming at the defects and shortcomings in the prior art, the invention aims to provide the W/O type compound tea polyphenol nanoemulsion which is fat-soluble, stable in property, less than 100nm in particle size and enhanced in oxidation resistance.
Meanwhile, the invention also provides a preparation method and application of the compound tea polyphenol nanoemulsion.
In order to achieve the purpose, the invention adopts the technical scheme that:
the compound tea polyphenol nanoemulsion is prepared from the following raw materials in parts by mass:
1 to 20 percent of tea polyphenol
1 to 15 percent of pomegranate peel polyphenol
15 to 50 percent of composite surfactant
1 to 15 percent of absolute ethyl alcohol
10 to 25 percent of oil
The balance of deionized water
The sum of the mass percentages of the raw materials is 100 percent.
The compound tea polyphenol nanoemulsion also comprises apple polyphenol which accounts for 1-15% by mass.
The preferable mass ratio of the compound tea polyphenol nanoemulsion raw materials is as follows:
the tea polyphenol accounts for 10 to 20 percent
1 to 5 percent of pomegranate peel polyphenol
1 to 5 percent of apple polyphenol
15 to 27 percent of composite surfactant
5 to 10 percent of absolute ethyl alcohol
15 to 20 percent of oil
The balance of deionized water
The sum of the mass percentages of the raw materials is 100 percent.
The composite surfactant is a mixture formed by mixing any one of soybean lecithin, Tween80, polyoxyethylene 20, polysorbate and glyceryl monostearate with span80, and is mixed according to a ratio of 2: 1-5: 1.
Further defined, the oil is any one of food grade liquid paraffin, soybean oil, peanut oil, rapeseed oil, olive oil and isopropyl myristate or a mixture of the oil and the peanut oil in any proportion.
Further limited, the compound tea polyphenol nanoemulsion is W/O type, the particle size is between 40.07-60.4 nm, the average particle size is 44.5nm, the emulsion encapsulation rate is 96-98%, and the viscosity is 236.39-412.9.
The preparation method of the compound tea polyphenol nanoemulsion comprises the steps of mixing the compound surfactant, absolute ethyl alcohol and oil according to a proportion, slowly stirring at room temperature, dropwise adding tea polyphenol, pomegranate polyphenol and deionized water into a system after the components are uniformly mixed, uniformly stirring, and standing until the solution is clear and unchanged to obtain the compound tea polyphenol nanoemulsion.
The compound tea polyphenol nanoemulsion is applied to health care products as an antioxidant.
The compound tea polyphenol nanoemulsion is applied to beauty products as an antioxidant.
The compound tea polyphenol nanoemulsion provided by the invention has the advantages that the preparation method is simple, the tea polyphenol and the pomegranate bark polyphenol are compounded to generate a synergistic effect, the formed nanoemulsion emulsion droplets have small particles, uniform distribution, small viscosity and good fluidity, the stability of the tea polyphenol in food and pharmaceutical preparations is greatly improved, the application range of the tea polyphenol nanoemulsion is expanded, the raw materials are non-toxic, safe and non-irritant, meanwhile, the tea polyphenol nanoemulsion is uniform in distribution, transparent in system and good in stability, the average particle size is 44.5nm, the tea polyphenol content of the formed compound tea polyphenol nanoemulsion reaches more than 4.8%, in addition, the pomegranate bark polyphenol is added, so that the compound tea polyphenol nanoemulsion has more remarkable and lasting oxidation resistance which is 2.78 times of that of an aqueous solution state, has better fat solubility, and is suitable for being widely applied to the fields of food, medicine, beauty treatment, and the like.
Drawings
Fig. 1 is a color contrast diagram of compound tea polyphenol nanoemulsion with different concentrations.
FIG. 2 is a comparison of compound tea polyphenol nanoemulsion dyed by Sudan red and methylene blue.
FIG. 3 is a comparison of the compound tea polyphenol nanoemulsion diluted by water phase and oil phase.
Fig. 4 is the microstructure of the compound tea polyphenol nanoemulsion.
Fig. 5 is a particle size distribution diagram of the compound tea polyphenol nanoemulsion.
FIG. 6 is a color contrast diagram of aqueous solution of tea polyphenols and nanoemulsion.
FIG. 7 is a graph comparing the oxidation resistance of aqueous tea polyphenol solution and nanoemulsion.
FIG. 8 is a comparison graph of DPPH free radical scavenging rate of tea polyphenol water solution or/and pomegranate polyphenol water solution and nanoemulsion of the tea polyphenol water solution and/or pomegranate polyphenol water solution.
Detailed Description
The technical solution of the present invention will be further explained with reference to the drawings and examples, and the present invention is not limited to the following implementation cases.
The compound tea polyphenol nanoemulsion is prepared by the following method:
adding the composite surfactant, absolute ethyl alcohol and the oil phase into a beaker according to a proportion, slowly stirring at room temperature (25 ℃), after the components are uniformly mixed, dropwise adding the tea polyphenol, the pomegranate polyphenol and the deionized water according to the formula ratio into the system, stirring and uniformly mixing, standing, and obtaining the compound tea polyphenol nanoemulsion after the solution is clear and unchanged.
Wherein, the total amount of the tea polyphenol is 100 percent, the pomegranate peel polyphenol is 1 to 20 percent, the pomegranate peel polyphenol is 1 to 15 percent, the composite surfactant is 15 to 50 percent, the absolute ethyl alcohol is 1 to 15 percent, the oil is 10 to 25 percent, and the balance is deionized water.
And when tea polyphenol and pomegranate polyphenol are added, 1-15% of apple polyphenol can be added into the formula, and the addition of the apple polyphenol further improves the oxidation resistance of the compound tea polyphenol nanoemulsion.
The composite surfactant is prepared from any one of soybean lecithin, Tween80, polyoxyethylene 20, polysorbate and glyceryl monostearate and span80 according to the volume ratio of 2-5: 1 to form a mixture.
The anhydrous ethanol is anhydrous ethanol.
The oil is selected from one or more of food grade liquid paraffin, soybean oil, peanut oil, rapeseed oil, olive oil, and isopropyl myristate.
Example 1
The compound tea polyphenol nanoemulsion of the embodiment comprises the following raw materials in percentage by weight: 15% of tea polyphenol, 5% of pomegranate peel polyphenol, 20% of composite surfactant, 10% of absolute ethyl alcohol, 20% of isopropyl myristate and the balance of deionized water, wherein the total amount is 100%.
The composite surfactant is formed by mixing Tween80 and span80 according to the volume ratio of 2: 1.
Example 2
The compound tea polyphenol nanoemulsion of the embodiment comprises the following raw materials in percentage by weight: 10% of tea polyphenol, 3% of pomegranate peel polyphenol, 27% of composite surfactant, 5% of absolute ethyl alcohol, 15% of isopropyl myristate and the balance of deionized water, wherein the total amount is 100%.
Wherein the composite surfactant is formed by mixing polyoxyethylene 20 and span80 according to the volume ratio of 3: 1.
Example 3
The compound tea polyphenol nanoemulsion of the embodiment comprises the following raw materials in percentage by weight: 20% of tea polyphenol, 1% of pomegranate peel polyphenol, 1% of apple polyphenol, 15% of composite surfactant, 10% of absolute ethyl alcohol, 20% of olive oil and the balance of deionized water, wherein the total amount is 100%.
The compound surfactant is formed by mixing polysorbate and span80 according to the volume ratio of 4: 1.
Example 4
The compound tea polyphenol nanoemulsion of the embodiment comprises the following raw materials in percentage by weight: 1% of tea polyphenol, 15% of pomegranate peel polyphenol, 15% of apple polyphenol, 15% of composite surfactant, 1% of absolute ethyl alcohol, 15% of soybean oil and olive oil mixed oil and the balance of deionized water, wherein the total amount is 100%.
Wherein the composite surfactant is formed by mixing soybean lecithin and span80 according to the volume ratio of 2: 1.
Wherein the mixing volume ratio of the soybean oil to the olive oil is 1: 2.
Example 5
The compound tea polyphenol nanoemulsion of the embodiment comprises the following raw materials in percentage by weight: 15% of tea polyphenol, 10% of pomegranate peel polyphenol, 10% of apple polyphenol, 20% of composite surfactant, 5% of absolute ethyl alcohol, 10% of mixture of liquid paraffin and peanut oil and the balance of deionized water, wherein the total amount is 100%.
Wherein the composite surfactant is formed by mixing glyceryl monostearate and span80 according to the volume ratio of 5: 1.
Wherein the liquid paraffin is food-grade liquid paraffin, and the mixing volume ratio of the food-grade liquid paraffin to the peanut oil is 2: 1.
Example 6
The compound tea polyphenol nanoemulsion of the embodiment comprises the following raw materials in percentage by weight: 18% of tea polyphenol, 5% of pomegranate peel polyphenol, 5% of apple polyphenol, 50% of composite surfactant, 1% of absolute ethyl alcohol, 10% of isopropyl myristate and peanut oil, and the balance of deionized water, wherein the total amount is 100%.
Wherein the composite surfactant is formed by mixing soybean lecithin and span80 according to the volume ratio of 2: 1.
Wherein the volume ratio of isopropyl myristate to peanut oil is 10: 1.
Using human liver cell HL-7702 as model, and separating by High Performance Liquid Chromatography (HPLC)Analyzing and comparing the absorptivity of HL-7702 cells on tea polyphenol water solution, tea polyphenol nanoemulsion and compound tea polyphenol nanoemulsion, and respectively mixing the tea polyphenol water solution, tea polyphenol nanoemulsion, compound tea polyphenol nanoemulsion and HL-7702 cells at 37 deg.C and 5% CO2After co-culturing for 24 hours in the incubator, catechin which is a main component of tea polyphenol is taken as a detection object.
HPLC detection results show that the concentration of the catechin in HL-7702 cells co-cultured with the compound tea polyphenol nanoemulsion is 1-2 times of that of the catechin in HL-7702 cells co-cultured with the tea polyphenol nanoemulsion, and the concentration of the catechin in HL-7702 cells co-cultured with the tea polyphenol nanoemulsion is 3-4 times of that in tea polyphenol water solution co-cultured cells.
The result shows that the somatic cells have better absorption rate for the compound tea polyphenol nanoemulsion. In addition, the DPPH method is used for analyzing and comparing the oxidation resistance of the tea polyphenol, the tea polyphenol nanoemulsion and the compound tea polyphenol nanoemulsion, and the compound tea polyphenol nanoemulsion is found to have 2.78 times of the DPPH free radical clearance rate of the single tea polyphenol water solution.
Therefore, the compound tea polyphenol nanoemulsion can be used as an antioxidant to be applied to health care products and beauty products, can be used as an effective component of the health care products and the beauty products, improves the oxidation resistance of the health care products or the beauty products, greatly improves the cell absorption rate of human bodies, and enhances the health care or beauty effect.
In order to verify the beneficial effects of the compound tea polyphenol nanoemulsion, the inventor conducts a large number of experiments to verify, and the following experiments are taken as examples for explanation.
1. Tea polyphenol nanoemulsion and preparation of compound tea polyphenol nanoemulsion
Adding the composite surfactant, absolute ethyl alcohol and the oil phase into a beaker according to a proportion, slowly stirring at room temperature (25 ℃), after the components are uniformly mixed, dropwise adding deionized water into the system, and obtaining a transparent blank nano-emulsion reagent when the solution is clear. And then, replacing deionized water with tea polyphenol and pomegranate polyphenol water solution with different proportions to prepare the W/O type compound tea polyphenol nanoemulsion, wherein the compound tea polyphenol nanoemulsion is shown in a figure (1).
As can be seen from figure 1, the tea polyphenol nanoemulsion is light yellow, and the color of the nanoemulsion becomes darker as the concentration of tea polyphenol increases from left to right.
The quality of the compound tea polyphenol nanoemulsion is evaluated by measuring the encapsulation efficiency, the viscosity and the pH value, the encapsulation efficiency of the compound tea polyphenol nanoemulsion is 96-98%, the viscosity is 236.39-412.9 (blank nanoemulsion viscosity 233.87), and the pH value is 6.9-7.2 (blank nanoemulsion pH value is 7).
2. W/O type tea polyphenol nanoemulsion identification
2.1 type of nanoemulsion judged by dyeing method
Methylene blue is a water-soluble dye which is blue and is easy to diffuse in a water phase; sudan red is an oil-soluble dye which is red and easily diffused in an oil phase. The type of the nanoemulsion can be judged according to the diffusion speed of methylene blue and Sudan red in the nanoemulsion. In the nanoemulsion, if the blue dye diffuses faster than the red dye, the nanoemulsion is O/W type, otherwise the nanoemulsion is W/O type, and if the blue dye diffuses as fast as the red dye, the nanoemulsion is bicontinuous. The tea polyphenol nanoemulsion is shown in the specification after being dyed by Sudan red and methylene blue respectively.
As can be seen from FIG. 2, the diffusion rate of Sudan red solution (left in FIG. 2) in tea polyphenol nanoemulsion is significantly higher than that of methylene blue solution (right in FIG. 2), indicating that the tea polyphenol nanoemulsion solution is W/O type.
2.2 determination of nanoemulsion type by dilution method
Diluting the nanoemulsion with water phase and oil phase respectively, and if the nanoemulsion can be diluted by a large amount of water phase without layering and is kept in a transparent state, the nanoemulsion is O/W type nanoemulsion; if the nano emulsion is diluted by a large amount of oil phase, no layering exists, and the transparent state is kept, the nano emulsion is W/O type nano emulsion. The tea polyphenol nanoemulsion is shown in figure 3 after being diluted by water phase and oil phase.
As can be seen from fig. 3, the tea polyphenol nanoemulsion solution diluted in the water phase (fig. 3 left) is significantly layered, while the tea polyphenol nanoemulsion solution diluted in the oil phase (fig. 3 right) is not layered, so that it can be determined that the tea polyphenol nanoemulsion solution is W/O type.
3. Tea polyphenol nanoemulsion microstructure and particle size detection
The microstructure and the grain size of the tea polyphenol nanoemulsion are detected by adopting a transmission electron microscope and a laser particle sizer. The results are shown in FIGS. 4 and 5.
After the detection of the transmission electron microscope (figure 4), the liquid drop is in a sphere-like shape, has good dispersibility and is not adhered. The diameter distribution of the particles is 40.07-60.4 nm and the average particle diameter is 44.5nm when the particles are detected by a laser particle size analyzer (figure 5).
4. Light stability test
Filling a proper amount of prepared tea polyphenol nanoemulsion into a colorless and transparent small glass bottle which is transparent, sealing, placing the bottle under normal illumination conditions for 30 hours, observing whether unstable phenomena such as layering, turbidity or crystal precipitation occur or not, and measuring the L (brightness), a (red-green) and b (yellow-blue) values of the bottle by using a colorimeter. The results are shown in FIG. 6.
As shown by comparison in FIG. 6, no delamination, turbidity or crystal precipitation occurred in each of the aqueous solution and the nanoemulsion after 30 hours of standing. The L, a and b values of the tea polyphenol nanoemulsion are lower than those of a tea polyphenol water solution, and the reduction values are smaller after pomegranate polyphenol is compounded. The tea polyphenol aqueous solution is yellow, can be photolyzed after illumination, the yellow becomes light, the b value is reduced violently, and the b value is reduced after the tea polyphenol aqueous solution is prepared into the nanoemulsion, which shows that the tea polyphenol nanoemulsion has better light stability than the tea polyphenol aqueous solution.
5. Experiment on Oxidation resistance
5.1 concentration of catechin in HL-7702 human liver cell
The absorption rates of the tea polyphenol water solution, the tea polyphenol nanoemulsion and the compound tea polyphenol nanoemulsion are analyzed and compared by measuring the concentration of catechin in HL-7702 human liver cells, and the test result is shown in figure 7.
As can be seen from FIG. 7, the concentration of intracellular catechin of HL-7702 co-cultured with the compound tea polyphenol nanoemulsion is 1-2 times of that of the intracellular catechin of HL-7702 co-cultured with the tea polyphenol nanoemulsion, and the concentration of intracellular catechin of HL-7702 co-cultured with the tea polyphenol nanoemulsion is 3-4 times of that of the intracellular catechin co-cultured with the tea polyphenol aqueous solution.
The results show that the liver cells have better absorption rate for the compound tea polyphenol nanoemulsion.
5.2 clearance of DPPH free radical
The antioxidant capacity of the tea polyphenol aqueous solution or/and the pomegranate polyphenol aqueous solution and the nanoemulsion of the tea polyphenol aqueous solution and/or the pomegranate polyphenol aqueous solution is analyzed and compared by measuring the scavenging rate of DPPH free radicals, and the test result is shown in figure 8.
As can be seen from fig. 8, the aqueous solution and nanoemulsion of tea polyphenols can effectively remove DPPH free radicals, and the rate of removing DPPH free radicals by the tea polyphenols nanoemulsion is 2.78 times that of the aqueous solution of tea polyphenols. After the pomegranate polyphenol is compounded, the oxidation resistance of the tea polyphenol aqueous solution and the nanoemulsion can be improved.
The results show that the compound tea polyphenol nanoemulsion has stronger oxidation resistance than the tea polyphenol water solution.

Claims (6)

1. The compound tea polyphenol nanoemulsion is characterized by being prepared from the following raw materials in parts by mass:
1 to 20 percent of tea polyphenol
1 to 15 percent of pomegranate peel polyphenol
15 to 50 percent of composite surfactant
1 to 15 percent of absolute ethyl alcohol
10 to 25 percent of oil
The balance of deionized water
The sum of the mass percentages of the raw materials is 100 percent;
the composite surfactant is a mixture formed by mixing any one of soybean lecithin, Tween80, polyoxyethylene 20, polysorbate and glyceryl monostearate with span80 in a ratio of 2: 1-5: 1;
the oil is any one or mixture of food-grade liquid paraffin, soybean oil, peanut oil, rapeseed oil, olive oil and isopropyl myristate in any proportion;
the compound tea polyphenol nanoemulsion is W/O type, the particle size of the compound tea polyphenol nanoemulsion ranges from 40.07 nm to 60.4nm, the average particle size is 44.5nm, the emulsion encapsulation rate is 96% -98%, and the viscosity is 236.39-412.9.
2. The compound tea polyphenol nanoemulsion of claim 1, wherein: also comprises apple polyphenol which accounts for 1 to 15 percent by mass.
3. The compound tea polyphenol nanoemulsion of claim 2, wherein the mass ratio of the raw materials is as follows:
the tea polyphenol accounts for 10 to 20 percent
1 to 5 percent of pomegranate peel polyphenol
1 to 5 percent of apple polyphenol
15 to 27 percent of composite surfactant
5 to 10 percent of absolute ethyl alcohol
15 to 20 percent of oil
The balance of deionized water
The sum of the mass percentages of the raw materials is 100 percent.
4. The method for preparing the compound tea polyphenol nanoemulsion of claim 1, which is characterized in that: mixing the composite surfactant, absolute ethyl alcohol and oil according to a proportion, slowly stirring at room temperature, after the components are uniformly mixed, dropwise adding tea polyphenol, pomegranate peel polyphenol and deionized water into the system, uniformly stirring, standing until the solution is clear and unchanged, and obtaining the compound tea polyphenol nanoemulsion.
5. The use of the compound tea polyphenol nanoemulsion of claim 1 as an antioxidant in health products.
6. The use of the compound tea polyphenol nanoemulsion of claim 1 as an antioxidant in cosmetic products.
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Title
Preparation on Nanoemulsion of Pomegranate Peel Polyphenol;傅力等;《Agricultural Science & Technology》;20151215;第12卷(第16期);第2821-2823页 *
茶多酚纳米级微粒的制备技术及其应用研究进展;何金岚等;《食品科学》;20110815;第32卷(第15期);第317-322页 *

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