CN107375153A - A kind of corn rudiment extract and its application in skin preparations for extenal use - Google Patents
A kind of corn rudiment extract and its application in skin preparations for extenal use Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/75—Anti-irritant
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Abstract
The invention discloses a kind of preparation method of corn rudiment extract, the described method comprises the following steps：A) corn seed is provided；B) sprout, rudiment 17 days, obtains corn rudiment respectively；C) heated-air drying, normal temperature ultrasonic extraction；D) extract solution obtained in step c) is filtered；E) filtrate obtained in step d) is concentrated, volatilizes solvent, obtain corn rudiment extract.Invention additionally discloses application of the corn rudiment extract in skin moisture-keeping, skin anti-inflammatory, alleviation skin irritatin and alleviation pruitus.
Description
Technical field
The invention belongs to cosmetics preparation field, more particularly to a kind of preparation method of corn rudiment extract and the extraction
The purposes of thing, for skin nursing.Present invention relates particularly to the corn rudiment extract in moisturizing, anti-inflammatory, alleviation stimulation, anti-scabies
Purposes in the skin preparations for extenal use itched.
Background technology
Skin refers to tissue of the body surface bag outside muscle, is the maximum organ of human body, mainly carries protection body
Body, perspire, feel the functions such as cold and hot and pressure.Skin covers whole body, and it is micro- from cause of disease to protect each tissue and organ in human body
The invasion and attack of the mechanical damage such as the external substances such as biology and physics, effectively resist environmental stimuli；Skin also maintains human body
The loss of moisture, electrolyte and nutriment etc., the stabilization of human internal environment is effectively maintained, be the natural screen for protecting human body
Barrier.
The water content of skin is very high, accounts for the 18-20% of whole body weight.The skin emolliency of health, smooth, high resilience,
Largely be attributed to the fact that moisture sufficient in skin, when moisture content of skin be less than 10% when, just occur dry, it is coarse even
Be chapped from the cold, and environmental drying, skin water oil is unbalance, cleaning excessively etc. reason can all cause skin dehydration.The excessive dehydration of skin can be broken
Bad skin barrier, prevent it from playing a part of being sufficiently reserved moisture.Skin without the moisture of abundance, can cause skin to be done for a long time
It is dry, accelerate skin aging, various skin problems then occur；In addition, moisture content of skin is sufficient or improves skin life
Manage environment, promote the prerequisite of skin metabolism.Therefore, keep the moistening of skin particularly heavy in skin daily nursing
Will.
Hyaluronic acid (HA) is referred to as optimal NMF, be in extracellular matrix (ECM) content at most, institute
The maximum composition of accounting example, has very strong water binding ability and viscosity, and master is played maintaining moisture of skin and elasticity etc.
The effect wanted；Meanwhile the glycosaminoglycan structures that hyaluronic acid is special, the moisture evaporation of skin can be prevented.It is inherent or external
Phenomena such as reduction of HA contents caused by factor, can cause skin elasticity to reduce, pachylosis, and wrinkle is formed.Therefore, keep or
The HA contents for improving skin are one of important channels for maintaining healthy skin care, by determining the HA contents in Skin Cell,
Evaluable skin moisture-keeping efficiency.
With the enhancing of people's living standard and self maintenance consciousness, except the basic nursing effect to skin such as moisturizing
Outside, the various skin problems that sensitiveness skin is brought cause the extensive concern of people.Sensitive is a kind of special skin
Skin type, refer to skin when by environmental stimuli, the nbjective symptoms such as erythema, papule, telangiectasis easily occur, adjoint itch,
The subjective symptoms such as shouting pain, calcination, tight feeling.Sensitive skin is mainly characterized by more drying, is easily tight and rubescent, by light
Microstimulation, you can produce itch, furfur and even burn and feel with shouting pain etc., severe patient can produce unhealthy emotion or psychosis
Shape.
The origin cause of formation of sensitive skin have it is a variety of, it is main including barrier function defect, nerves reaction is too strong or inflammatory reaction
Vicious circle, antiallergic activity is divided into barrier function maintenance, neuroleptic on this basis and suppresses the type of inflammation 3.With just
Normal skin is compared, and sensitive skin has the characteristics of barrier function damage or abnormality of nerve function, it more difficult in resisting environmental stimuli thing
With the invasion and attack of anaphylactogen, a series of scytitis caused by stimulations or allergy easily occurs reacts, and then further damages skin
Barrier structure and nerve endings, cause vicious circle.Therefore, daily nursing and improvement are carried out to sensitive skin, inflammation of releiving, is delayed
Skin irritatin and itch etc. are solved, has active promoting function to improving sensitiveness skin.
Lipopolysaccharides (Lipopolysaccharides, LPS), is called endotoxin, is that gram-negative cells cell institute is peculiar
A kind of composition.Lipopolysaccharides only just discharges after bacterial death dissolves or destroys bacterium cell by artificial means, acts on
Macrophage, cause inflammatory reaction.Macrophage is a kind of white blood cell in tissue, belongs to immunocyte, exempts from body
Play an important roll in epidemic disease response and inflammatory reaction.During inflammatory reaction, macrophage is under bacterium and its endotoxic stimulation, meeting
Secretion largely has endogenous chemicals --- the inflammatory factor of proinflammatory effect, such as nitric oxide (NO), tumor necrosis factor
Son-α (TNF-α) and interleukin-1 beta (lL-1 β) etc., these inflammatory factors again can further activated macrophage, activation
Macrophage afterwards can discharge a large amount of NO, can cause the injury of cell and the diastole that blood vessel is excessive, eventually cause serious inflammation
Reaction and complication.NO plays the adjustment effect of key in inflammation and immune response, suppressed huge as a kind of inflammatory mediator
NO release, can reduce inflammation to a certain extent caused by phagocyte activation.In addition, NO can be formed with super oxide anion
Oxidation nitroso ion and be decomposed into supervirulent OH, once into target cell or close on cell and will cause cytotoxicity
Effect even injury tissue.Therefore, reducing activated macrophage, excessively release NO has certain anti-inflammatory meaning.To macrophage
The inhibitory action research of release inflammatory mediator is the common method of the anti-delayed-type hypersensitivity effect of detectable substance confrontation.
Lauryl sodium sulfate (Sodium lauryl sulfate, SLS) is used as anion surfactant, is a kind of
Representative skin irritatin material, it is combined with cell membrane can suppress cell metabolism and destroy cell membrane, so as to destroy
The barrier function of cuticula, makes stimulant be easy to skin permeation, causes skin abnormality to react.When stimulant is reached by cuticula
Cortical tissue, change and balanced inside cell membrane, influence cell propagation and differentiation, induce the release of inflammatory mediator；Stimulant exists
Skin barrier is destroyed in keratinocyte, causes inflammatory factor IL-1 α etc. release, promotes cell degeneration, death.Therefore,
Sample, the influence that evaluate sample discharges to cell viability and inflammatory factor are handled with SLS, the anti-inflammatory of evaluable sample eases up
Solve stimulus equivalence energy.
Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 (substance P, SP) is to find earliest neuropeptide in the world, is a kind of neuropeptide of high activity
Material, it is widely distributed in nervous system and other peripheral tissues' organs, there are different physiological roles.SP in skin is main
From Sensory nerve fibre tip, minority is by histocyte such as keratinocyte (KC), mast cell, mononuclear macrophage
Synthesized Deng cell.SP can promote epithelial cells release IL-1, IL-8, TNF-α, promote mononuclear macrophage to produce and discharge
IL-1, IL-6, TNF-α and IFN-γ, increase highly active T cell and produce IL-2 and expression IL-2 acceptors, stimulate mast cell
TNF-α is produced, neutrophil leucocyte and vascular endothelial cell produce II-8, and borrow the effect of these cell factors, influence and adjust
Control cutaneous immunisation and inflammatory reaction.Research shows that SP is a kind of important itch medium, can be by activating the direct of NK1 acceptors
Mode, or the indirect mode by promoting other itch media to discharge, participate in itch mechanism, so as to cause the redness of skin and scabies
Itch.
Bradykinin (bradykinin, BK) is a kind of kassinin kinin that it can be combined with bradykinin receptor B1 and B2, injection of skin
Being combined after bradykinin by the bradykinin receptor B2 with nerve fibre, make periphery thin vessels expansion, capillary permeability strengthens,
There is strong impulse effect to pain nerve tip simultaneously, so as to cause the inflammatory reactions such as the redness of skin, scabies itching and pain,
Therefore it is usually used in causing itch model as material incentive.
Interleukin-6 (Interleukin-6, IL-6), also referred to as Pro-inflammatory mediator, it is a kind of huge by monokaryon
The cell factor with multiple functions of phagocyte secretion, can pass through humoral and cellular immune response function effect inflammation, host defense
And tissue damage, it is one of medium of acute reaction, and one of important proinflammatory factor.Chemotactic factor (CF) is mainly by white
It is that one kind has chemotactic and activation to inflammatory cell secreted by the histocytes such as cell, epidermal cell and fibroblast
Cell factor, and interleukin 8 (Interleukin-8, IL-8) be then most important neutrophil chemotactic because
Son, mainly attract neutrophil leucocyte, eosinophil, basophilic granulocyte and T cell etc., be that neutrophil cell passes through blood
Endothelial tube reaches an important factor for inflammation part.IL-6 and IL-8 is immune and inflammatory reaction important regulatory factor, in skin
In skin inflammatory reaction, IL-6 is one of cell factor caused by keratinocyte (Keratinocyte, KC), is had to KC propagation
Facilitation；And IL-8 is then inflammatory chemokine important in scytitis reaction.Excessive IL-6 and IL- in cell tissue
8 can cause the inflammation damnification of skin, therefore, suppress the secretion of the inflammatory factors such as IL-6 and IL-8 to a certain extent, can be effective
Suppress the generation of inflammatory reaction, improve inflammation and the allergic reaction of skin.Research is it has also been found that the cell factor such as IL-6 and IL-8 exists
Played an important role in the pathophysiological process of inflammatory cutaneous itch, be usually used in the factor of pruritis analysis detection.
Seed, which is sprouted, refers to a series of orderly physiology courses and morphogenetic process of the seed since imbitition, is
Crucial transition stage in plant life history.Plant physiology research shows that the seed respiration in sprouting is accelerated, enzymatic activity
Enhancing, metabolic activity is vigorous, and the organic matter such as organic matter conversion, reserve substance starch, fat, protein of a series of complex occurs
Matter experienced the metabolic transformations such as a series of plant physiology hydrolysis, transport and reconstruction.It is a large amount of insoluble in this transition process
Small-molecule substance that the macromolecular substances generation of solution dissociates, simple.Compared with macromolecular, free small-molecule substance dissolubility
It is good, it is more easy to be absorbed by organisms.This explanation germination treatment can improve the nutritive value of seed, especially beans seed corn, through germination
Nutritive value can be greatly improved after processing.After corn germination processing, lysine, tryptophan, water soluble vitamin and calcium content are bright
Aobvious to improve, protein digestibility significantly improves, and viscosity reduces, so as to improve the energy density of corn.
Corn (Classification system：Zea mays L.) it is important cereal crops and forage crop, there is " gold in the world
The title of crop ".It contains abundant nutriment, particularly protein, fat, carbohydrate, cellulose, inorganic salts, vitamin etc.
Content is quite big, while also has invigorating the spleen benefit containing advanced nutrients such as abundant oleic acid, linoleic acid, lutein, glutathione
Stomach, hypoglycemic defaecation, anti-aging, anti-cancer, avoid constipation, anti-atherosclerosis etc. act on.Has great-hearted corn seed through dormancy
Latter stage of ripening, give required external condition (temperature, moisture, illumination and nutriment), you can into the sprouting stage.Corn rudiment rank
Section, starch is decomposed into maltose, then is hydrolyzed to monose；Lipolysis is glycerine and aliphatic acid；Breaks down proteins are amino acid.This
Small molecule carbohydrate and amino acid after a little conversions help to maintain moisture of skin, aliphatic acid and glycerine can supplement natural sebum into
Point, form natural skin sparing barrier.There is very high beautifying nourishing to be worth for this explanation corn rudiment, and effect may be better than
Corn.
At present, there is the macrophage release NO that can suppress lipopolysaccharide-induced there is no literature reported on corn rudiment, suppress
The HaCaT cell deaths of SLS inductions, HaCaT Hemapoiesis inflammatory factors IL-1 α for suppressing SLS inductions, suppress bradykinin induction
NHF Hemapoiesis inflammatory factors IL-8, suppress Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 induction NHF Hemapoiesis inflammatory factor IL-6/8 and promotion
The activity of HaCaT Hemapoiesis hyaluronic acids.
The present invention investigates it and is suppressing macrophage release NO, the suppression of LPS inductions first using corn rudiment as research object
The HaCaT cell deaths of SLS inductions processed, HaCaT Hemapoiesis inflammatory factors IL-1 α for suppressing SLS inductions, suppression bradykinin lure
NHF Hemapoiesis inflammatory factors IL-6/8, the NHF Hemapoiesis inflammatory factor IL-6/8 of suppression Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 induction led, and promote
Enter the activity of HaCaT Hemapoiesis hyaluronic acids, and present invention firstly discovers that corn rudiment is suppressing the HaCaT of SLS inductions
Cell death, HaCaT Hemapoiesis inflammatory factors IL-1 α for suppressing SLS inductions, the NHF Hemapoiesis for suppressing bradykinin induction
Inflammatory factor IL-6/8, the NHF Hemapoiesis inflammatory factors IL-6/8 for suppressing Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 induction etc. effect are better than non-rudiment
Corn seed, if by its development and application in the cosmetics such as anti-inflammatory, antipruritic and moisturizing, it will have different skin care experience.
The content of the invention
The present inventor have passed through extensively and found after in-depth study：Corn rudiment extract, which has, to be protected
Wet, anti-inflammatory, alleviate the dependent interactions such as stimulation, antipruritic.Therefore, corn rudiment extract can be added to as functional additive
In skin preparations for extenal use, especially cosmetics, for moisturizing, anti-inflammatory, alleviate stimulation and antipruritic.
On the one hand, the invention provides a kind of preparation method of corn rudiment extract, the described method comprises the following steps：
A) corn seed is provided；
B) sprout, rudiment 1-7 days, obtains corn rudiment respectively；
C) heated-air drying, normal temperature ultrasonic extraction；
D) extract solution obtained in step c) is filtered；
E) filtrate obtained in step d) is concentrated, volatilizes solvent, obtain corn rudiment extract.
In some preferred embodiments, above-mentioned steps a) includes washing corn seed, removes the seed of floating.
In some preferred embodiments, at a temperature of above-mentioned steps b) is included in 15-25 DEG C, 70-90%'s is relative
Under humidity, carried out under dark conditions.
In some preferred embodiments, above-mentioned steps b) sprouts 2-3 days.
In some preferred embodiments, above-mentioned steps c) uses volumetric concentration 60-80% ethanol, and preferably 70%
Ethanol extracts.
On the other hand, the invention further relates to the corn rudiment extract being prepared according to the method for the invention.
On the other hand, the invention further relates to the corn rudiment extract being prepared according to the method for the invention in skin
Application in moisturizing, skin anti-inflammatory, alleviation skin irritatin and/or alleviation pruitus.
The invention further relates to a kind of skin preparations for extenal use, and it includes the corn rudiment being prepared according to the method for the invention
Extract.
In some preferred embodiments, percentage by weight of the corn rudiment extract in skin preparations for extenal use is
0.0001%-20% (w/w), preferable percentage by weight are 0.001%-10% (w/w), and preferred percentage by weight is
0.001%-5% (w/w), most preferred percentage by weight are 0.01%-5% (w/w).
Detailed description of the invention
More significantly alleviate stimulation than corn extract present invention firstly discovers that corn rudiment extract has, suppress scabies
The effect itched, it is preferable to sprouting the corn rudiment extract of 1~7 day, it is more preferred to sprout the corn of 1~3 day
Rudiment extract, optimal is the corn rudiment extract of sprouting 2~3 days.
The invention provides one kind to have skin moisture-keeping, skin anti-inflammatory, alleviate skin irritatin and/or alleviate pruitus work(
The corn rudiment extract of effect.Present invention firstly discovers that corn rudiment extract has moisturizing, anti-inflammatory, alleviates stimulation, antipruritic
The effect of, it is suitable as functional additive and is used in skin preparations for extenal use.
The invention provides a kind of preparation method of corn rudiment extract, its preparation comprises the following steps：
A) corn seed is provided；
B) sprout, rudiment 1-7 days, obtains corn rudiment respectively；
C) heated-air drying, normal temperature ultrasonic extraction；
D) extract solution obtained in step c) is filtered；
E) filtrate obtained in step d) is concentrated, volatilizes solvent, obtain corn rudiment extract.
In some specific embodiments, step a) includes washing corn seed.For example, (such as it can be purified with water
Water, deionized water etc.) washing corn seed.By washing, the seed swum on above can be removed, leave and be deposited in bottom
Seed.Follow-up sprouting step is then deposited in the seed of bottom using these.
In some specific embodiments, step b) is carried out at a temperature of 15-25 DEG C.In some specific embodiment party
In formula, relative humidity of the step b) in 70-90% is carried out.In some specific embodiments, step b) is under dark conditions
Carry out.In some preferred embodiments, the Germination Temperature of the maize bud can be 15-20 DEG C, and more preferably temperature is 20
℃.In some preferred embodiments, the sprouting relative humidity of the maize bud is 80%.In addition, in order to avoid seed is withered
It is dry, it can continue to provide water (such as purified water, deionized water etc.), the moisture enough to seed and nutrition during germination process.
In some specific embodiments, sprout time can be any of 1,2,3,4,5,6,7 day.Another
In a little specific embodiments, sprout time can be 1-7 days, preferably 1-4 days.In other specific embodiments, sprout
It can be 2-6 days, more preferably 2-4 days, most preferably 2-3 days to send out the time.
In some specific embodiments, step c) drying is carried out using air drier.For example, the hot air drying
Dry temperature is 40-60 DEG C, and preferable temperature is 50 DEG C.In addition, normal temperature condition includes 25 ± 5 DEG C of temperature range.
In some specific embodiments, the material ratio of step c) extract is 1:5 to 1:15, preferred feed ratio is
1:10.In some specific embodiments, Extraction solvent is 60-80% ethanol (volumetric concentration), and preferred solvent is 70% second
Alcohol (volumetric concentration).In some specific embodiments, ultrasonic extraction temperature is 20-30 DEG C, and preferably ultrasonic extraction temperature is
25℃.In some specific embodiments, the ultrasonic extraction time is 2-4 hours, and the preferably ultrasonic extraction time is 3 hours.
In some specific embodiments, step c) filtering can be sieving, suction filtration, filter paper filtering.It is specific at some
Embodiment in, step c) filtering is filtered using filter paper, it is for instance possible to use HYUNDAI No.53 filter paper is filtered.
In some specific embodiments, step d) condensing modes are to be concentrated under reduced pressure, and preferably thickening temperature is 45 DEG C.
Present invention firstly discovers that corn rudiment extract has skin moisture-keeping effect, keratinocyte can be promoted to generate
The activity of hyaluronic acid.Therefore, corn rudiment extract can be added in cosmetics as the additive with moisture-keeping efficacy, risen
Acted on to skin moisture-keeping.
In some preferred embodiments, skin moisture-keeping effect is realized using the corn rudiment extract sprouted 2-7 days.
In some preferred embodiments, concentration is used to realize moisturizing for 50-200 μ g/ml corn rudiment extract
Effect.
Present invention firstly discovers that corn rudiment extract has skin antiinflammatory action, lipopolysaccharide-induced macrophage can be suppressed
Cell discharges NO.Therefore, corn rudiment extract can be added in cosmetics as the additive with anti-inflammatory efficacy, play skin
Skin antiinflammatory action.
In some preferred embodiments, skin anti-inflammatory efficacy is realized using the corn rudiment extract sprouted 1-3 days.
In some preferred embodiments, concentration is used to realize anti-inflammatory for 50-200 μ g/ml corn rudiment extract
Effect.In preferred embodiment, concentration is used to realize anti-inflammatory efficacy for 100 μ g/ml corn rudiment extract
Present invention firstly discovers that corn rudiment extract, which has, alleviates skin irritation, the angle of SLS inductions can be suppressed
Matter forms cell death.Therefore, corn rudiment extract can be as with the additive additionization for alleviating skin irritatin effect
In cosmetic, the effect of releiving skin irritatin is played.
In some preferred embodiments, realized using the corn rudiment extract sprouted 1-7 days and alleviate skin irritatin
The effect of.In some preferred embodiments, realized using the corn rudiment extract for sprouting 4-7 and alleviate skin irritatin
Effect.In most preferred embodiments, the effect of alleviating skin irritatin is realized using the corn rudiment extract sprouted 7 days.
In some preferred embodiments, use concentration to be realized for 50-200 μ g/ml corn rudiment extract and alleviate
The effect of skin irritatin.In preferred embodiment, concentration is used to be realized for 200 μ g/ml corn rudiment extract slow
Solve skin irritatin effect.
Present invention firstly discovers that corn rudiment extract, which has, alleviates skin irritation, the angle of SLS inductions can be suppressed
Matter forms Hemapoiesis IL-1 α.Therefore, corn rudiment extract can add as with the additive for alleviating skin irritatin effect
Enter in cosmetics, play the effect of releiving skin irritatin.
In some preferred embodiments, realized using the corn rudiment extract sprouted 1-6 days and alleviate skin irritatin
The effect of.In some preferred embodiments, realized using the corn rudiment extract for sprouting 4-6 and alleviate skin irritatin
Effect.In most preferred embodiments, the effect of alleviating skin irritatin is realized using the corn rudiment extract sprouted 4 days.
In some preferred embodiments, use concentration to be realized for 50-200 μ g/ml corn rudiment extract and alleviate
The effect of skin irritatin.In preferred embodiment, use concentration real for 100-200 μ g/ml corn rudiment extract
Now alleviate skin irritatin effect.
Present invention firstly discovers that corn rudiment extract, which has, alleviates pruitus effect, bradykinin induction can be suppressed
Fibroblast discharges inflammatory factor IL-6/8 and/or suppresses the fibroblast release inflammatory factor IL-6/8 of Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 induction.
Therefore, corn rudiment extract can be added in cosmetics as with the additive for alleviating pruitus effect, play anti-scabies
The effect of itching.
In some preferred embodiments, realized using the corn rudiment extract sprouted 2-3 days and alleviate skin irritatin
The effect of.
In some preferred embodiments, use concentration to be realized for 1 μ g/ml corn rudiment extract and alleviate skin thorn
The effect of swashing.
Therefore, the application the invention further relates to corn rudiment extract in skin moisture-keeping.In some specific embodiment party
In formula, the skin moisture-keeping effect is realized by promoting keratinocyte to generate the activity of hyaluronic acid.
The invention further relates to application of the corn rudiment extract in skin anti-inflammatory.In some specific embodiments,
The skin antiinflammatory action is realized by suppressing lipopolysaccharide-induced macrophage release NO.
The invention further relates to application of the corn rudiment extract in skin irritatin is alleviated.In some specific embodiments
In, the skin irritation of alleviating is that the keratinocyte of SLS inductions is dead and/or suppression SLS is induced by suppressing
Keratinocyte generation IL-1 α are realized.
The invention further relates to application of the corn rudiment extract in pruitus is alleviated.In some specific embodiments
In, it is described alleviate pruitus effect be by suppress bradykinin induce fibroblast discharge inflammatory factor IL-6/8 and/
Or suppress the fibroblast release inflammatory factor IL-6/8 realizations of Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 induction.
In another aspect of this invention, there is provided a kind of skin preparations for extenal use with skin moisture-keeping effect, outside the skin
The corn rudiment extract and the acceptable excipient of cosmetic field being prepared according to the inventive method are included with agent.
In another aspect of this invention, there is provided a kind of skin preparations for extenal use with skin anti-inflammatory efficacy, outside the skin
The corn rudiment extract and the acceptable excipient of cosmetic field being prepared according to the inventive method are included with agent.
In another aspect of this invention, there is provided a kind of skin preparations for extenal use that can alleviate skin irritatin, outside the skin
The corn rudiment extract and the acceptable excipient of cosmetic field being prepared according to the inventive method are included with agent.
In another aspect of this invention, there is provided a kind of skin preparations for extenal use that can alleviate pruitus, outside the skin
The corn rudiment extract and the acceptable excipient of cosmetic field being prepared according to the inventive method are included with agent.
Corn rudiment extract can be applied to skin preparations for extenal use, especially can be applied in cosmetics, according to preparation not
Same type adds different dosages.
Corn rudiment extract of the present invention can be as the additive application with moisture-keeping efficacy in external preparation for skin
Agent.In a specific embodiment, corn rudiment extract can be as the additive application with moisture-keeping efficacy in change
In cosmetic.In a specific embodiment, the cosmetics are selected from：Mildy wash, toner, emulsion, cream, gel, face
Film.Different dosages is added according to the different type of preparation.
Corn rudiment extract of the present invention can be as the additive application with anti-inflammatory efficacy in external preparation for skin
Agent.In a specific embodiment, corn rudiment extract can be as the additive application with anti-inflammatory efficacy in change
In cosmetic.In a specific embodiment, the cosmetics are selected from：Mildy wash, toner, emulsion, cream, gel, face
Film.Different dosages is added according to the different type of preparation.
Corn rudiment extract of the present invention can as with alleviate skin irritatin effect additive application in
Skin preparations for extenal use.In a specific embodiment, corn rudiment extract, which can be used as to have, alleviates skin irritatin effect
Additive application in cosmetics.In a specific embodiment, the cosmetics are selected from：Mildy wash, toner, breast
Liquid, cream, gel, facial mask.Different dosages is added according to the different type of preparation.
Corn rudiment extract of the present invention can as with alleviate pruitus effect additive application in
Skin preparations for extenal use.In a specific embodiment, corn rudiment extract, which can be used as to have, alleviates pruitus effect
Additive application in cosmetics.In a specific embodiment, the cosmetics are selected from：Mildy wash, toner, breast
Liquid, cream, gel, facial mask.Different dosages is added according to the different type of preparation.
The skin preparations for extenal use of corn rudiment extract containing the present invention falls within protection scope of the present invention.
The skin preparations for extenal use is typically used for the general designation concept of all the components outside skin, such as can be cosmetic preparation
Composition or pharmaceutical compositions.Can be foundation make up material, facial dressing cosmetic preparation, body in the cosmetic combination
Makeup material, hair nursing cosmetic preparation etc., can reasonable selection according to different purposes to its formulation without specifically limited.
Bu Tong also allowed in the cosmetic combination according to formulation and purpose containing different cosmeceutical aspects
Medium or matrix excipients.
It can be used for cosmetics, dermatology or the pharmaceutically acceptable excipient of Dermatologic preparation composition of the present invention
For the form of aqueous phase, oil phase, gel, water bag wax pattern emulsion, emulsion oil-in-water or water-in-oil emulsion.Aqueous phase is a kind of or more
The mixture of water-soluble or dispersed component is planted, it can be liquid, semisolid or solid under room temperature (25 DEG C).Excipient bag
The form of suspension in water or water-alcohol excipient, dispersion liquid or solution is included or can be, it can contain thickener or solidifying
Jelly.The suitable product form of knowledge-chosen that those skilled in the art can be grasped based on those skilled in the art, wherein wrapping
The component contained.
Described composition can include aqueous phase, and the aqueous phase can contain water or water and at least one hydrophilic organic solvent
Mixture, described hydrophilic organic solvent such as alcohol, the rudimentary unitary of straight or branched especially containing 2-5 carbon atom
Alcohol, such as ethanol or propyl alcohol；Polyalcohol, such as propane diols, sorbierite, glycerine, panthenol or polyethylene glycol and its mixture.
When the composition of invention is emulsion form, said composition can also optionally include surfactant.
Described composition can also include film forming polymer, such as polyurethanes, polyacrylic acid homopolymer or copolymerization
Thing, polyester, resin and/or silicone resin based on hydrocarbon.It can dissolve a polymer in or be scattered in the acceptable tax of cosmetics
Optionally merge in shape agent and with plasticizer.
The composition of the present invention can also include oil phase, and described oil phase contains under room temperature (25 DEG C) for the oily molten of liquid
Property or oil-dispersing property component and/or be oily or wax-like material at room temperature, such as wax, semisolid, natural gum and its mixture.Should
Oil phase can also contain organic solvent.
It is generally liquid at room temperature, suitable oily matter includes：From the oil based on hydrocarbon of animal, such as perhydrogenating
Squalene；Vegetable oil based on hydrocarbon, such as the triglyceride of the C4-10 aliphatic acid of liquid, such as enanthic acid or Trivent OCG
Class, or oil, such as sunflower oil, corn oil, soybean oil, grape-kernel oil, castor oil, avocado oil, caprylic/capric triglyceride
Class, jojoba oil；The straight or branched hydro carbons of mineral or synthesis source, such as liquid paraffin and its derivant, vaseline；Synthesis
The esters of esters and ethers, particularly fatty alcohol, such as isopropyl myristate, palmitic acid 2- ethylhexyls, stearic acid 2- are pungent
Base dodecyl ester, isostearyl isostearate ester；Hydroxylating esters, for example, lactic acid isooctadecanol ester, octyl hydroxystearate,
Octyl hydroxystearate, hydroxy stearic acid octyldodecyl, the enanthic acid esters of fatty alcohol, sad esters and capric acid lipid；
Polyalcohol esters, such as propylene, the heptanoate of neopentyl glycol two, the isononoate of diethylene glycol (DEG) two and pentaerythritol ester
Class；Aliphatic alcohols containing C12-26, such as octyldodecanol, 2- butyl octanol, 2- hexyl decyl alcohols, 2- undecyls 15
Alkanol, oleyl alcohol；Fluorocarbon oil and/or fluorosilicon oil based on part hydrocarbon, silicone oil, it is at room temperature liquid or semisolid volatility or non-
Volatile straight chain or cyclo-methicone, such as X 2-1401 and dimethyl silicone polymer, its is optional
Include phenyl, such as Silicone DC 556, siloxanes and its mixture.
The composition of the present invention can be further comprising any component being usually used in cosmetic field.These components include
Preservative, water phase thickener (extract biopolymer, synthetic polymer) and fatty phase thickener, aromatic, hydrophily and
Lipophilic active agent and its mixture.
The composition of the present invention can also include other particle phase, and described particle can be mutually in cosmetic composition
The pigment and/or pearling agent and/or filler used.
Pigment may reside in composition, and suitable inorganic pigment includes titanium oxide, zirconium oxide and cerium oxide and oxygen
Change zinc, iron oxide and barba hispanica；Suitable organic pigment includes barium, strontium, calcium and aluminum lake and carbon black.
Pearling agent may reside in composition, and suitable pearling agent includes being coated with titanium oxide, iron oxide or natural face
The mica of material.
Filler may reside in composition, and suitable filler includes talcum powder, silica, zinc stearate, cloud
Mother, kaolin, nylon powder, polyethylene powders, Teflon, starch, borazon, copolymer microsphere, such as silicone resin
Microballon.
The oil phase of the present composition can include one or more waxes, natural gum or its mixture.Wax is included based on hydrocarbon
Wax, fluorine wax and/or siloxane wax, and plant, mineral, animal and/or synthesis source can be derived from.Suitable wax includes honeybee
Wax, Brazil wax, candelila wax, paraffin, microwax, ceresine；Synthetic wax includes Tissuemat E, the siloxanes containing C16-45
Wax.Natural gum is generally dimethyl silicone polymer or sodium carboxymethylcellulose or extraction species, and semi-solid material is generally base
In the compound of hydrocarbon, such as lanolin and its derivative.
The composition of the present invention can be configured to any suitable product form.This kind of product form includes, but unlimited
In aerosol spray, creme, emulsion, solid, liquid, dispersion, foam, gel, toner, mousse, ointment, pulvis,
Patch, brilliantine, solution, hand press pump-type spray, club, facial mask and hygenic towelette.The composition of the present invention can be passed through
Various methods well-known in the art are advantageously available for preparing or produced as cosmetics, dermatology or medicine local application
Product.
The Dermatologic preparation composition of the present invention can include one or more following ingredients：It is anti-allergic agent, antimicrobial
Agent, antioxidant, chelating agent, colouring agent depigmentation, emollient, emulsifying agent, excoriation agent, film forming agent, spices, moisturizing
It is agent, insect repellent, lubricant, pharmaceutically active agents, humidizer, photostabilizer, preservative, skin conditioner, skin penetration enhancer, anti-
Shine agent, stabilizer, surfactant, thickener, viscosity modifier, vitamin or its any combination.
Embodiment
Corn rudiment extract is not directed in document has moisturizing, anti-inflammatory, alleviation skin irritatin and/or pruitus
Effect, do not refer to as with moisturizing, anti-inflammatory, alleviation skin irritatin and/or the additive addition for alleviating pruitus yet
In cosmetics.Therefore, there is moisturizing, anti-inflammatory present invention firstly provides corn rudiment extract, alleviate skin irritatin and/or skin
The effect of itch, while the extract can also be used as functional additive to add skin preparations for extenal use, especially in cosmetics.
The present invention is expanded on further with reference to specific embodiment.It should be appreciated, however, that these embodiments are only used for
It is bright the present invention and be not meant to limit the scope of the invention.The test method of unreceipted actual conditions in the following example, generally
According to normal condition, or according to the condition proposed by manufacturer.Unless otherwise indicated, all percentage and number be by weight
Meter.
The corn used in embodiment is GuangPyeong corns, and original producton location is South Korea, passes through " YeLim
Corporation " suppliers purchase seed.
The experimental method, it is conventional method unless otherwise specified；The reagent and material, unless otherwise specified,
Commercially obtain.
Comparative example：The preparation of maize seed extract
Take a certain amount of dry corn seed, 10 times of 70% ethanol (volumetric concentration) normal temperature ultrasonic extraction 3h, extract solution filter paper
(HYUNDAI No.53) is filtered, and is concentrated into rotary evaporator low temperature (45 DEG C) powdered, obtains maize seed extract.
This experiment corn is GuangPyeong corns, and original producton location is South Korea, by the way that " YeLim Corporation " are supplied
Business purchases seed.
Embodiment 1：The preparation of 1 day extract of corn rudiment
Corn seed purifies water washing, removes the seed swum on the water surface, only with the seed for being deposited in the bottom.In temperature
For 20 ± 5 DEG C, humidity 80%, sprouted under dark conditions.Air drier (temperature is adjusted to 50 DEG C) dries rudiment maize seed
Son.Take the corn seed of the rudiment 1 day of a certain amount of drying, 10 times of 70% ethanol (volumetric concentration) normal temperature ultrasonic extraction 3h, extraction
Liquid filter paper (HYUNDAI No.53) filter, be concentrated into rotary evaporator low temperature (45 DEG C) it is powdered, obtain corn rudiment extraction
Thing.
Embodiment 2：The preparation of 2 days extracts of corn rudiment
Corn seed purifies water washing, removes the seed swum on the water surface, only with the seed for being deposited in the bottom.In temperature
For 20 ± 5 DEG C, humidity 80%, sprouted under dark conditions.Air drier (temperature is adjusted to 50 DEG C) dries rudiment maize seed
Son.Take the corn seed of the rudiment 2 days of a certain amount of drying, 10 times of 70% ethanol (volumetric concentration) normal temperature ultrasonic extraction 3h, extraction
Liquid filter paper (HYUNDAI No.53) filter, be concentrated into rotary evaporator low temperature (45 DEG C) it is powdered, obtain corn rudiment extraction
Thing.
Embodiment 3：The preparation of 3 days extracts of corn rudiment
Corn seed purifies water washing, removes the seed swum on the water surface, only with the seed for being deposited in the bottom.In temperature
For 20 ± 5 DEG C, humidity 80%, sprouted under dark conditions.Air drier (temperature is adjusted to 50 DEG C) dries rudiment maize seed
Son.Take the corn seed of the rudiment 3 days of a certain amount of drying, 10 times of 70% ethanol (volumetric concentration) normal temperature ultrasonic extraction 3h, extraction
Liquid filter paper (HYUNDAI No.53) filter, be concentrated into rotary evaporator low temperature (45 DEG C) it is powdered, obtain corn rudiment extraction
Thing.
Embodiment 4：The preparation of 4 days extracts of corn rudiment
Corn seed purifies water washing, removes the seed swum on the water surface, only with the seed for being deposited in the bottom.In temperature
For 20 ± 5 DEG C, humidity 80%, sprouted under dark conditions.Air drier (temperature is adjusted to 50 DEG C) dries rudiment maize seed
Son.Take the corn seed of the rudiment 4 days of a certain amount of drying, 10 times of 70% ethanol (volumetric concentration) normal temperature ultrasonic extraction 3h, extraction
Liquid filter paper (HYUNDAI No.53) filter, be concentrated into rotary evaporator low temperature (45 DEG C) it is powdered, obtain corn rudiment extraction
Thing.
Embodiment 5：The preparation of 5 days extracts of corn rudiment
Corn seed purifies water washing, removes the seed swum on the water surface, only with the seed for being deposited in the bottom.In temperature
For 20 ± 5 DEG C, humidity 80%, sprouted under dark conditions.Air drier (temperature is adjusted to 50 DEG C) dries rudiment maize seed
Son.Take the corn seed of the rudiment 5 days of a certain amount of drying, 10 times of 70% ethanol (volumetric concentration) normal temperature ultrasonic extraction 3h, extraction
Liquid filter paper (HYUNDAI No.53) filter, be concentrated into rotary evaporator low temperature (45 DEG C) it is powdered, obtain corn rudiment extraction
Thing.
Embodiment 6：The preparation of 6 days extracts of corn rudiment
Corn seed purifies water washing, removes the seed swum on the water surface, only with the seed for being deposited in the bottom.In temperature
For 20 ± 5 DEG C, humidity 80%, sprouted under dark conditions.Air drier (temperature is adjusted to 50 DEG C) dries rudiment maize seed
Son.Take the corn seed of the rudiment 6 days of a certain amount of drying, 10 times of 70% ethanol (volumetric concentration) normal temperature ultrasonic extraction 3h, extraction
Liquid filter paper (HYUNDAI No.53) filter, be concentrated into rotary evaporator low temperature (45 DEG C) it is powdered, obtain corn rudiment extraction
Thing.
Embodiment 7：The preparation of 7 days extracts of corn rudiment
Corn seed purifies water washing, removes the seed swum on the water surface, only with the seed for being deposited in the bottom.In temperature
For 20 ± 5 DEG C, humidity 80%, sprouted under dark conditions.Air drier (temperature is adjusted to 50 DEG C) dries rudiment maize seed
Son.Take the corn seed of the rudiment 7 days of a certain amount of drying, 10 times of 70% ethanol (volumetric concentration) normal temperature ultrasonic extraction 3h, extraction
Liquid filter paper (HYUNDAI No.53) filter, be concentrated into rotary evaporator low temperature (45 DEG C) it is powdered, obtain corn rudiment extraction
Thing.
Embodiment 8：Influence to the HaCaT cell deaths of SLS inductions
Using Human keratinocytes (Human Keratinocyte) HaCaT, (German cancer research institute DKFZ is carried for this experiment
For) experiment detection is carried out, using DMEM (the Dulbecco's Modified containing 10%FBS (fetal bovine serum)
Eagle's Medium) culture medium, in 37 DEG C, 5%CO2Cultivated in constant incubator.
HaCaT cells are with 1.5x 104/ hole concentration is inoculated in 96 orifice plates, cultivates 24h.Culture medium is removed, FBS washings, is used
The cell of starvation is made in DMEM culture mediums (serum free medium) without FBS.Second day SLS with 20 μ g/ml and suitable
When the given the test agent processing of concentration, 24h is cultivated.
The WST-1 reagents of 10 times of dilutions are added in culture medium, after each hole adds 100 μ L, 2h is cultivated, is read by ELISA
Number instrument (Thermo, 51119300) determines absorbance OD at 450nm.Each embodiment sample is calculated according to formula (1) to lure SLS
The HaCaT cell death inhibiting rates led.
Cell death inhibiting rate (%)=(ODSample﹣ ODSLS)/(ODControl﹣ ODSLS) * 100%
Formula (1)
Table：The influence for the HaCaT cell deaths that 1 different embodiments are induced SLS
The influence result (table 1) of the HaCaT cell deaths induced from different embodiments SLS：It is 200 μ in concentration
During g/ml, HaCaT cell deaths of the embodiment 1-7 to SLS inductions has different degrees of suppression, embodiment 1-7 inhibiting rate
Comparative example is superior to, increasing trend is presented in embodiment 4-7 inhibiting rate, and the suppression efficiency of embodiment 7 is optimal.Illustrate to sprout 1~7
It corn rudiment can be good at alleviating skin irritatin, and effect is better than non-rudiment corn seed.
Embodiment 9：The influence generated to the HaCaT cell IL-1 α of SLS inductions
Using Human keratinocytes (Human Keratinocyte) HaCaT, (German cancer research institute DKFZ is carried for this experiment
For) experiment detection is carried out, using DMEM (the Dulbecco's Modified containing 10%FBS (fetal bovine serum)
Eagle's Medium) culture medium, in 37 DEG C, 5%CO2Cultivated in constant incubator.
HaCaT cells are with 1.5x 104/ hole concentration is inoculated in 96 orifice plates, cultivates 24h.Culture medium is removed, FBS washings, is used
The cell of starvation is made in DMEM culture mediums (serum free medium) without FBS.Second day SLS with 10 μ g/ml and suitable
When the given the test agent processing of concentration, 24h is cultivated.
Tested using the ELISA KIT (DY200) of R＆D systems, extinction is determined at 450nm with ELISA readout instruments
Spend OD.The inhibiting rate for the HaCaT Hemapoiesis IL-1 α that each embodiment sample is induced SLS is calculated according to formula (2).
IL-1 α generation inhibiting rates (%)=(ODSLS﹣ ODSample)/(ODSLS﹣ ODControl) * 100%
Formula (2)
Table 2：Influence of the different embodiments in the HaCaT cell IL-1 α generations induced SLS
The influence result (table 2) generated by different embodiments under various concentrations to the HaCaT cell IL-1 α of SLS inductions
Understand：HaCaT Hemapoiesis IL-1 α unrestraint of the comparative example to SLS inductions under various concentrations acts on, and embodiment 1-6
There is different degrees of inhibitory action to the HaCaT Hemapoiesis IL-1 α of SLS inductions under various concentrations, embodiment 4-6 suppresses
Effect lifting, the inhibitory action of embodiment 4 are optimal.The corn rudiment for illustrating to sprout 1~6 day can be good at alleviating skin irritatin,
And effect is better than non-rudiment corn seed.
Embodiment 10：The influence generated to the IL-6/8 of BK inductions
This experiment carries out experiment detection using Normal human fibroblast NHF (being provided by LONZA), using containing penicillin
(100IU/mL) and streptomysin (100 μ g/mL), supplement reagent (insulin 0.1%, rhFGF-B 0.1%, GA-1000
0.1%, FBS 2%) fibroblast culture medium (FBM), in 37 DEG C, 5%CO2Cultivated in constant incubator.
NHF cells are with 1.5x 104/ hole concentration is inoculated in 48 orifice plates, cultivates 24h.Cultivated with the DMEM without supplement reagent
The cell of starvation is made in base (serum free medium).Second day embodiment sample with 10nM BK debita spissitudos is handled, and
Cultivate 24h.
After being tested with the ELISA KIT (DY206, DY208) of R＆D systems, surveyed with ELISA readout instruments at 450nm
Determine absorbance OD, the inhibiting rate for the NHF Hemapoiesis IL-6/8 that each embodiment sample is induced BK is calculated according to formula (3).
IL-6/8 generation inhibiting rates (%)=(ODBK﹣ ODSample)/(ODBK﹣ ODControl) * 100%
Formula (3)
Table 3：The influence for the NHF Hemapoiesis IL-6/8 that different embodiments are induced BK
Influence (table 3) result that the IL-6/8 induced from different embodiments BK is generated：It is 1 μ g/ in sample concentration
During ml, the IL-6 generations that comparative example is induced BK with embodiment 2-3 have inhibitory action, but embodiment 2-3 inhibitory action is excellent
In comparative example；When sample concentration is 1 μ g/ml, the IL-8 generation unrestraints that comparative example is induced BK act on, and embodiment 2-3
There is inhibitory action to the IL-8 generations of BK inductions.Suppress BK induction IL-6/8 generation can alleviate pruitus, illustrate sprouting 2~
The maize bud of 3 days has alleviates pruitus effect well, and effect is better than non-rudiment corn seed.
Embodiment 11：The influence generated to the IL-6/8 of SP inductions
This experiment carries out experiment detection using Normal human fibroblast NHF (being provided by LONZA), using containing penicillin
(100IU/mL) and streptomysin (100 μ g/mL), supplement reagent (insulin 0.1%, rhFGF-B 0.1%, GA-1000
0.1%, FBS 2%) fibroblast culture medium (FBM), in 37 DEG C, 5%CO2Cultivated in constant incubator.
NHF cells are with 1.5x 104/ hole concentration is inoculated in 48 orifice plates, cultivates 24h.Cultivated with the DMEM without supplement reagent
The cell of starvation is made in base (serum free medium).Second day with the embodiment sample of 500nM SP and debita spissitudo
Reason, and cultivate 5h.
After being tested with the ELISA KIT (DY206, DY208) of R＆D systems, surveyed with ELISA readout instruments at 450nm
Determine absorbance OD, the inhibiting rate for the NHF Hemapoiesis IL-6/8 that each embodiment sample is induced SP is calculated according to formula (4).
IL-6/8 generation inhibiting rates (%)=(ODsp﹣ ODSample)/(ODsp﹣ ODControl) * 100%
Formula (4)
Table 4：The influence for the NHF Hemapoiesis IL-6/8 that different embodiments are induced SP
The NHF Hemapoiesis IL-6/8 induced from different embodiments SP influence (table 4) result：Concentration is 1 μ g/
During ml, the IL-6 generations that comparative example is induced SP have facilitation, and the IL-6 generations that embodiment 2-3 is induced SP have suppression to make
With；When concentration is 1 μ g/ml, the IL-8 generations that comparative example is induced SP have facilitation, the IL-8 that embodiment 2-3 is induced SP
Generation has inhibitory action.And the IL-6/8 generations for suppressing SP inductions can alleviate pruitus, illustrate to sprout the corn of 2~3 days
Bud can be good at alleviating skin irritatin, and effect is better than non-rudiment corn seed.
Embodiment 12：Initial cell is stimulated lipopolysaccharides (LPS) to discharge the influence of nitric oxide (NO)
This experiment carries out experiment inspection using the mononuclear macrophage strains of mouse RAW 264.7 (offer of bank of Korea Cell system)
Survey, using DMEM (the Dulbecco's Modified Eagle's containing 10%FBS (fetal bovine serum)
Medium) culture medium, in 37 DEG C, 5%CO2Cultivated in constant incubator.
The cells of RAW 264.7 are with 2.5x 104/ hole concentration is inoculated in 96 orifice plates, cultivates 24h.Culture medium is removed, FBS is washed
Wash, the cell of starvation is made in the DMEM culture mediums (serum free medium) without FBS.Second day LPS with 1 μ g/mL and
The sample treatment of debita spissitudo, cultivate 24h.
Using Griess methods detection NO burst size.After cell culture medium and the Griess reagents of same amount are mixed at room temperature
Reaction 10 minutes.OD values are determined at 540nm with ELISA readout instruments (Thermo, 51119300).Calculated according to formula (5) each
The inhibiting rate for the macrophage release NO that embodiment sample is induced LPS.
NO release inhibiting rates (%)=(ODLPS﹣ ODSample)/(ODLPS﹣ ODControl) * 100%
Formula (5)
Table 5：The influence for the macrophage release NO that different embodiments are stimulated LPS
Initial cell is stimulated LPS to discharge NO influence (table 5) result from different embodiments：Sample concentration is 100 μ
During g/ml, embodiment 1-3, which stimulates LPS initial cell to discharge NO, different degrees of inhibitory action.Illustrate to sprout 1~3 day
Corn rudiment have a certain degree of antiinflammatory action.
Embodiment 13：Influence to HaCaT Hemapoiesis HA
Using Human keratinocytes (Human Keratinocyte) HaCaT, (German cancer research institute DKFZ is carried for this experiment
For) experiment detection is carried out, using DMEM (the Dulbecco's Modified containing 10%FBS (fetal bovine serum)
Eagle's Medium) culture medium, in 37 DEG C, 5%CO2Cultivated in constant incubator.
HaCaT cells are with 1.5x 104/ hole concentration is inoculated in 96 orifice plates, is put into incubator culture 24h.Remove culture medium,
FBS is washed, and the cell of starvation is made of the DMEM culture mediums (serum free medium) without FBS.Add various concentrations by
Test agent, cultivate 24h.
Tested using the ELISA KIT (DY3614) of R＆D systems, extinction is determined at 450nm with ELISA readout instruments
Spend OD.Promotion rate of each embodiment sample to HaCaT Hemapoiesis HA is calculated according to formula (6).
HA generation promotion rates (%)=(ODSample﹣ ODControl)/ODControl* 100%
Formula (6)
Table 6：Influence of the different embodiments under various concentrations to HaCaT Hemapoiesis HA
From different embodiments under various concentrations to HaCaT Hemapoiesis HA influence result (table 6)：Embodiment 2-
7 have facilitation under various concentrations to HaCaT Hemapoiesis HA, and concentration dependent is presented.Illustrate to sprout 2~7 days
Corn rudiment can remarkably promote the generation of HA in epidermal cell, so as to effectively pin skin moisture, play moisture-keeping function.
The corn rudiment extract prepared in Example 1-7, the preparation for skin preparations for extenal use.The skin preparations for extenal use
Preferably cosmetic composition, such as toner, Essence, creams etc..The dosage of the corn rudiment extract is
0.0001%-20% (w/w).Preferable percentage by weight is 0.01%-20% (w/w).Preferred percentage by weight is
0.01%-10% (w/w).Most preferred percentage by weight is 0.1%-5% (w/w).
It is the embodiment of the concrete application in the skin preparations for extenal use containing corn rudiment extract below, and its these formulations
Formula and preparation method."-" represents no added in following table.
Embodiment 14：The preparation of face cream
Embodiment 15：The preparation of emulsion
Embodiment 16：The preparation of gel
Embodiment 17：The preparation of toner
Embodiment 18：The preparation of Essence
Embodiment 19：The preparation of facial mask
Embodiment 20：The preparation of eye cream
Embodiment 21：The preparation of aerosol (cleaning bubble)
Embodiment 22：The preparation of spraying
Embodiment 23：The preparation of shower cream
Embodiment 24：The preparation of mildy wash
Claims (9)
1. a kind of preparation method of corn rudiment extract, the described method comprises the following steps：
A) corn seed is provided；
B) sprout, rudiment 1-7 days, obtains corn rudiment respectively；
C) heated-air drying, normal temperature ultrasonic extraction；
D) extract solution obtained in step c) is filtered；
E) filtrate obtained in step d) is concentrated, volatilizes solvent, obtain corn rudiment extract.
2. the method as described in claim 1, it is characterised in that the step a) includes washing corn seed, removes floating
Seed.
3. the method as described in claim 1, it is characterised in that at a temperature of the step b) is included in 15-25 DEG C, 70-
Under 90% relative humidity, carried out under dark conditions.
4. method as claimed in claim 3, it is characterised in that the step b) sprouts 2-3 days.
5. the method as described in claim 1, it is characterised in that the step c) uses volumetric concentration 60-80% ethanol, excellent
70% ethanol is selected to extract.
6. the corn rudiment extract that the method as described in claim any one of 1-5 is prepared.
7. corn rudiment extract as claimed in claim 6 is in skin moisture-keeping, skin anti-inflammatory, alleviation skin irritatin and/or delays
Solve the application in pruitus.
8. a kind of skin preparations for extenal use, it includes corn rudiment extract as claimed in claim 6.
9. skin preparation composition for external use as claimed in claim 8, it is characterised in that the corn rudiment extract is in external preparation for skin
Percentage by weight in agent is 0.0001%-20% (w/w), and preferable percentage by weight is 0.001%-10% (w/w), more excellent
The percentage by weight of choosing is 0.001%-5% (w/w), and most preferred percentage by weight is 0.01%-5% (w/w).
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108498426A (en) * | 2018-06-08 | 2018-09-07 | 上海家化联合股份有限公司 | A kind of barley rudiment extract and its application in skin preparations for extenal use |
| CN110227052A (en) * | 2019-06-19 | 2019-09-13 | 杭州悦萱堂化妆品有限公司 | Face cream and preparation method thereof is repaired in a kind of moisturizing |
| CN111840160A (en) * | 2020-08-11 | 2020-10-30 | 广州鹰远生物科技有限公司 | A composition containing lignum sappan extract and its application in skin external preparation |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102988242A (en) * | 2012-12-07 | 2013-03-27 | 上海相宜本草化妆品股份有限公司 | Method for preparing mung bean sprout extracting solution and application of mung bean sprout extracting solution |
| CN103371934A (en) * | 2012-04-13 | 2013-10-30 | 上海家化联合股份有限公司 | Preparation method and use of black soya bean sprout extracting solution |
-
2017
- 2017-09-18 CN CN201710841053.XA patent/CN107375153A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103371934A (en) * | 2012-04-13 | 2013-10-30 | 上海家化联合股份有限公司 | Preparation method and use of black soya bean sprout extracting solution |
| CN102988242A (en) * | 2012-12-07 | 2013-03-27 | 上海相宜本草化妆品股份有限公司 | Method for preparing mung bean sprout extracting solution and application of mung bean sprout extracting solution |
Non-Patent Citations (1)
| Title |
|---|
| 来吉祥等: "谷豆萌芽过程中成分变化、生物活性及应用研究进展", 《食品与生物技术学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108498426A (en) * | 2018-06-08 | 2018-09-07 | 上海家化联合股份有限公司 | A kind of barley rudiment extract and its application in skin preparations for extenal use |
| CN110227052A (en) * | 2019-06-19 | 2019-09-13 | 杭州悦萱堂化妆品有限公司 | Face cream and preparation method thereof is repaired in a kind of moisturizing |
| CN111840160A (en) * | 2020-08-11 | 2020-10-30 | 广州鹰远生物科技有限公司 | A composition containing lignum sappan extract and its application in skin external preparation |
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Application publication date: 20171124 |
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