CN107373340A - A kind of selenium-rich cordyceps oat steamed bun and preparation method thereof - Google Patents
A kind of selenium-rich cordyceps oat steamed bun and preparation method thereof Download PDFInfo
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- CN107373340A CN107373340A CN201710620319.8A CN201710620319A CN107373340A CN 107373340 A CN107373340 A CN 107373340A CN 201710620319 A CN201710620319 A CN 201710620319A CN 107373340 A CN107373340 A CN 107373340A
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- selenium
- yeast
- cordyceps
- steamed bun
- oat
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- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 title claims abstract description 88
- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 88
- 241000190633 Cordyceps Species 0.000 title claims abstract description 37
- 238000002360 preparation method Methods 0.000 title claims abstract description 24
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
- A23L7/104—Fermentation of farinaceous cereal or cereal material; Addition of enzymes or microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
- A23L31/10—Yeasts or derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/14—Yeasts or derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/16—Inorganic salts, minerals or trace elements
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Mycology (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Inorganic Chemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Bakery Products And Manufacturing Methods Therefor (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention relates to a kind of selenium-rich cordyceps oat steamed bun, it is prepared by the raw material including following composition:Cordyceps sinensis seed liquor or Chinese caterpillar fungus fermentation magma, oatmeal, wholewheat flour and Se-enriched yeast.Invention further provides the preparation method of the selenium-rich cordyceps oat steamed bun.The steamed bun made of the inventive method, homogeneous, into spongy, flexible, form is complete, has non-wrinkled surfaces, delicate mouthfeel, there is cordyceps sinensis oat and a milk fragrance, the physiologically active ingredient such as Organic Selenium containing yeast, cordyceps sinensis adenosine, cordycepin, isomalto-oligosaccharide in steamed bun.In addition, the also nutritional ingredient containing oat and wheat simultaneously, as carbohydrate, protein, dietary fiber, amino acid and vitamin, nutrient are more balanced.The present invention organically combines the physiologically active ingredient and nutritional ingredient of multiple beneficial health, people is eaten good things in daily life, eats health.
Description
Technical field
The present invention relates to food processing field, and in particular to a kind of selenium-rich cordyceps oat steamed bun and preparation method thereof.
Background technology
Steamed bun is one of traditional staple food of China resident, be China northeast northwest North China wide geographic area people almost daily from
The food do not opened.Traditionally, the making of steamed bun is to make raw material with wheat flour, is proofed with old face or active dry yeast as fermentation
Agent, through with face, fermentation, be molded, proof, steam and form.Wheat flour contains carbohydrate 70%, the crude protein of a large amount
12.7% and fatty 2.5%, dietary fiber content only has 3.7%.Although wheat flour steamed bun is containing abundant carbohydrate
And crude protein, but dietary fiber content is too low, lacks balanced nutritious and healthcare function.
In order to improve the nutrition of wheat steamed bun, the healthcare function of steamed bun is improved, in recent years, except making steamed bun with wheat
Outside, also there is the corn steamed bun that maize flour and wheat flour are mixed in market once in a while, and maize flour also contains substantial amounts of carbohydrate,
And crude protein, fat and dietary fiber are seldom.In recent years it has been proposed that making the method for raw material making steamed bun, Chinese patent with rice
CN201310123147.5 makees raw material with rice, through boiling, gelatinization, mechanical agitation, adds a certain amount of Gluten and sodium alginate,
Rice steamed bun is made with traditional steamed bun preparation method.Rice also contains substantial amounts of carbohydrate about 80% but crude protein
0.5%th, the content of crude fibre 0.4% is but very low.Wheat, rice and corn are all China resident staple food conventional foodstuffs, and they all contain
There is very Hi CHO, but crude protein content is relatively low, and particularly dietary fiber is lower, long-term consumption, nutrition mistake easily occurs
Weighing apparatus.Dietary fiber eats very few, there is also enteric microorganism population and deteriorates, influences enteron aisle and health.
One kind is proposed in order to supplement dietary fiber deficiency, Chinese patent CN 201310306775.7 in people's diet
Dietary fiber steamed bun, Testa Tritici (wheat bran) is fried, dispersion and fining, face filling is made after face filling, or sugaring spontaneous fermentation is made, then
Filling parcel in face made of wheat bran is molded with the musculus cutaneus for admixing yeast, finally fermented to proof, steam is steamed and is made, this meals
Fiber steamed bun is eaten into steamed bun, not as being specifically dietary fiber steamed stuffed bun.In addition, although wheat bran is left containing up to 30%
Right dietary fiber, but dietary fiber contained therein is mainly coarse insoluble cellulose, lignin, hemicellulose, is grown
The feed of herbivore is used as since phase, with simple mechanical crushing and spontaneous fermentation, it is difficult to it is modified, people largely eat
With the mucous membrane on gastrointestinal tract wall top layer may be damaged by the mechanism of coarse insoluble lignocellulosic.
Oat is one kind of wheat class, and oat is solvable also containing what is enriched in addition to containing substantial amounts of carbohydrate, protein
Property dietary fiber, is health food generally acknowledged both at home and abroad, but seldom as health product made of raw material using oat, and in the market is common
For oatmeal, but have no and be health-care steamed bread that raw material makes with oatmeal and related report.
The content of the invention
The deficiency that the present invention makes and applied for steamed bun, there is provided one kind has the balanced nutritious demand of people and health-care effect concurrently
Steamed bun.
Specifically, the invention provides a kind of selenium-rich cordyceps oat steamed bun, by the raw material preparation including following composition
Into:Cordyceps sinensis seed liquor or Chinese caterpillar fungus fermentation magma, oatmeal, wholewheat flour and Se-enriched yeast.
Cordyceps sinensis of the present invention is Cordyceps militaris or cordyceps sinensis.The Cordyceps militaris can be given by Shanghai East China University of Science and Technology.
The cordyceps sinensis is preferably aweto J3, is separated from the fresh cordyceps sinensis of Nagqu, and through morphological feature and DNA
Sequencing determines.
Specifically, the invention provides a kind of selenium-rich cordyceps oat steamed bun, in parts by weight, by including following composition
Raw material is prepared:100~450 parts of cordyceps sinensis oatmeal, 50~400 parts of wholewheat flour, 1.0~3.5 parts of Se-enriched yeast mud, milk powder
10~20 parts, 15~25 parts of sucrose, 7~15 parts of oligosaccharide;Wherein, the cordyceps sinensis oatmeal is by cordyceps sinensis seed liquor and oatmeal
After mixing, culture, dry, crush and obtain.
, it is necessary to add suitable quantity of water in actual fabrication process.The addition of the water is preferably cordyceps sinensis oatmeal and whole wheat
The 55~105% of flour total amount.
Cordyceps sinensis seed liquor of the present invention is the cordyceps or aweto access liquid that will be cultivated on slant medium
In body seed culture medium, rotating speed be 160~200 revs/min, temperature for 23~25 DEG C under the conditions of cultivate 2~3 days and obtain.It is excellent
Selection of land, contain in the seed culture medium:150~250g/L of potato, 10~20g/L of glucose, 5~10g/L of peptone.
Cordyceps sinensis oatmeal of the present invention is prepared by the method comprised the following steps:Oatmeal after sterilizing is accessed
Cordyceps sinensis seed liquor, while sterilized water is added, cultivate 2 under conditions of 23~25 DEG C, relative humidity 80~85% after stirring
~3 days, after stirring stirring up and down, continue culture 3~4 days, dry, crush, produce.Preferably, the addition cordyceps sinensis seed liquor
Quality account for the 10~30% of the oat tablet quality.
Preferably, the cordyceps sinensis oatmeal is to buy oatmeal that Australian import oat makes as raw material using market,
Load round, in 121~123 DEG C of 30~45min of sterilizing of temperature, it is (described to access the cordyceps sinensis seed culture fluid cultivated in advance
Add cordyceps sinensis seed liquor quality account for the 10~30% of the oat tablet quality), and add simultaneously sterilized water (it is described add it is sterile
The quality of water accounts for the 15~30% of the oat tablet quality), in 23~25 DEG C of temperature after stirring, relative humidity is 80~
85% culture 2~3 days, formed mycelia after, oat culture up and down stir stir once, continue culture 3~4 days, then
Oat culture obtains cordyceps sinensis oatmeal in 60~90 degree of drying and crushings.In the every gram of cordyceps sinensis oatmeal prepared in the method containing about
Cordyceps sinensis adenosine 1.29~3.25%, cordycepin 0.45~1.43%.
Se-enriched yeast mud of the present invention is that the S. cervisiae cultivated on slant medium containing selenium is inoculated into liquid ferment
Female seed culture medium, cultivated 18~20 hours under the conditions of 160~200 revs/min, 28~32 DEG C of rotating speed, obtain seed liquor;By institute
State seed liquor access liquid yeast fermentation medium, training of being ventilated under the conditions of 160~200 revs/min of rotating speed, 28~32 DEG C of temperature
Support, and the selenium solution of sterilizing added in growth late log phase, make the selenium element concentration in the fermentation medium reach 5~
15ug/ml, continue culture and (trained by 36~42 hours referred to herein as from the seed liquor is accessed into liquid yeast fermentation medium
Support to 36~42 hours), supernatant is removed after centrifugation, obtains Se-enriched yeast mud.
Preferably, the liquid yeast seed culture medium composition is:1~10g/L of glucose, 1~10g/L of yeast extract, egg
1~10g/L of white peptone, pH5.0~5.5;And/or the liquid yeast fermentation medium composition is:10~20g/L of glucose, ferment
Female 1~10g/L of cream, 1~10g/L of peptone, pH5.0~5.5.
The preparation method of above-mentioned selenium-rich cordyceps oat steamed bun may include following steps:Se-enriched yeast mud is mixed with water, then
Add other raw materials to be sufficiently mixed, stirring rubs up into dough, and shaping is split after fermentation, proofs, steams, produce.Preferably, it is described
Ferment for 3~5 hours of being fermented under the conditions of relative humidity 70~80%, 30~35 DEG C of temperature.
As a kind of parallel alternative solution of the present invention, the selenium-rich cordyceps oat steamed bun, in parts by weight, by including such as
The raw material of lower composition is prepared:150~200 parts of Chinese caterpillar fungus fermentation magma, 100~400 parts of oatmeal, wholewheat flour 100~400
Part, 0.5~1.0 part of Se-enriched yeast powder, 1.0~3.5 parts of active dry yeast, 10~20 parts of milk powder, 15~25 parts of sucrose, oligosaccharide
7~15 parts.
, it is necessary to add suitable quantity of water in actual fabrication process.The addition of the water is preferably 310~360 parts.
Chinese caterpillar fungus fermentation magma of the present invention is the cordyceps or aweto access that will be cultivated on slant medium
In liquid seed culture medium, it is 160~200 revs/min, is cultivated 2~3 days under the conditions of 23~25 DEG C of temperature in rotating speed, obtain seed
Liquid;By the seed liquor access liquid fermentation medium in, rotating speed be 160~200 revs/min, temperature be 23~25 DEG C of bars
Cultivated 4~5 days under part, obtain Chinese caterpillar fungus fermentation magma.Preferably, contain in the seed culture medium:1~10g/L of glucose, sucrose
10~50g/L, 1~5g/L of yeast extract, 1~10g/L of peptone, 0.1~1g/L of potassium dihydrogen phosphate;And/or the fermented and cultured
Contain in base:5~15g/L of glucose, 20~30g/L of sucrose, 1~10g/L of yeast extract, 1~10g/L of peptone.
Se-enriched yeast powder of the present invention is that the S. cervisiae cultivated on slant medium containing selenium is inoculated into liquid ferment
Female seed culture medium, cultivated 18~20 hours under the conditions of 160~200 revs/min, 28~32 DEG C of rotating speed, obtain seed liquor;By institute
State seed liquor access liquid yeast fermentation medium, training of being ventilated under the conditions of 160~200 revs/min of rotating speed, 28~32 DEG C of temperature
Support, and the selenium solution of sterilizing added in growth late log phase, make the selenium element concentration in the fermentation medium reach 5~
15ug/ml, continue culture and (trained by 36~42 hours referred to herein as from the seed liquor is accessed into liquid yeast fermentation medium
Support to 36~42 hours), supernatant is removed in centrifugation hypsokinesis, obtains Se-enriched yeast mud;After Se-enriched yeast mud drying, crushing,
Obtain Se-enriched yeast powder.Preferably, the liquid yeast seed culture medium composition is:1~10g/L of glucose, yeast extract 1~
10g/L, 1~10g/L of peptone, pH5.0~5.5;And/or the liquid yeast fermentation medium composition is:Glucose 10~
20g/L, 1~10g/L of yeast extract, 1~10g/L of peptone, pH5.0~5.5.
The preparation method of above-mentioned selenium-rich cordyceps oat steamed bun may include following steps:By Chinese caterpillar fungus fermentation magma and the dry ferment of activity
Mother is mixed to get leavening;After the leavening is mixed with water, add other raw materials and be sufficiently mixed, stirring rubs up into face
Group, shaping is split after fermentation, proofs, steams, produce.Preferably, it is described fermentation be relative humidity 70~80%, temperature 30~
Fermented under the conditions of 35 DEG C 3~5 hours.
The steamed bun of this offer, homogeneous, into spongy, flexible, form is complete, has non-wrinkled surfaces, and delicate mouthfeel, has
Cordyceps sinensis oat and milk fragrance, in steamed bun the physiologically active such as Organic Selenium containing yeast, cordyceps sinensis adenosine, cordycepin, isomalto-oligosaccharide into
Point, in addition, the also nutritional ingredient containing oat and wheat simultaneously, such as carbohydrate, protein, dietary fiber amino acid and dimension
Raw element, nutrient are more balanced.The physiologically active ingredient and nutritional ingredient of multiple beneficial health, organically combination exists the present invention
Together, people is eaten good things in daily life, eat health.
Selenium-rich cordyceps oat steamed bun provided by the invention, there is following healthcare function:
1) Cordyceps medicinal fungi, it is a kind of rare, rare Chinese medicine in China, medication is with a long history, and cordyceps sinensis contains cordyceps sinensis
The pharmaceutical chemistry material of a variety of physiologically actives such as adenosine, cordycepin, cordycepic acid, polysaccharide, cordyceps sinensis are used for the void of nourishing lung and kidney two, long
The diseases such as cough, bronchitis, impotence, irregular menstruation, high fat of blood, heart blood supply insufficiency, wherein, cordycepin can disturb cell RNA and DNA
Synthesis, suppress the abnormal cell division such as cancer cell, there is antitumor, strengthen immunity, HIV-I resisting type is viral, antimycotic
Deng pharmacological action.
2) selenium total selenium content in human body is about 1300mg, is one of necessary trace element of human body, selenium may participate in human body
The synthesis of liver GSH-PX activity peroxidase, important adjustment effect is played to the eubolism of human body, selenium can be removed in vivo
Free radical, improves body immunity and red blood cell carries the ability of oxygen.Heavy metal in selenium energy antagonist, there are dispelling toxicity, detoxicating, drop blood
Fat, hypotensive, cancer-resisting, anti-aging, prevent more than the 40 kinds of disease such as hepatic disease and Keshan disease.
3) dietary fiber includes soluble dietary fiber element, pectin and cellulose not soluble in water, lignin and hemicellulose
The dietary cellulosics such as element, it is the indispensable nutrient compositions of people's healthy diet.Dietary fiber is keeping the digestion of people's enteron aisle
In system health, play the role of important, dietary fiber can remove the waste of intestinal wall, accelerate the discharge of enteron aisle noxious material, warp
The enough edible dietary fibers of perseverance, can a variety of diseases such as prevention of cardiovascular disease, diabetes, cancer, constipation, hemorrhoid.
4) oligosaccharide is a kind of low molecule saccharide compound, and wherein isomalto-oligosaccharide is a kind of carbohydrate of oligosaccharide, by
Can not be by enzyme hydrolysis among stomach in oligosaccharide, and large intestine is directly entered, and be preferably intestinal beneficial bacterium Bifidobacterium Bifidum institute profit
With the breeding of Bifidobacterium Bifidum can be effectively promoted, so as to suppress C.perfringens in enteron aisle, Escherichia coli, salmonella, will
The breeding of the spoilage organisms such as Hayes bacterium, harmful bacteria, improve micro-ecological environment in human body, often edible adjustable functions of intestines and stomach, preventing and treating
Constipation, reduce cholesterol, improve lipid-metabolism, and there are the health-care effects such as cancer-resisting effect and pre- anti-caries.
Embodiment
Following examples are used to illustrate the present invention, but are not limited to the scope of the present invention.
Embodiment 1
The preparation of cordyceps sinensis oatmeal:
Take oatmeal 100g to be fitted into culture vessel at 122 DEG C of temperature the 35min that sterilizes, after cooling, be respectively connected to pupa worm
Grass or cordyceps sinensis seed culture fluid 20ml, sterilized water 23ml is added, stirs, then puts under 23-24 DEG C of environment of temperature, phase
Humidity 80-85% is cultivated 2.5 days, after growing mycelia, cordyceps sinensis oat culture stirred once, continues culture 5-10 days.
Wherein, seed culture medium composition is:Glucose 5g/L, sucrose 15g/L, yeast extract 3g/L, peptone 6g/L, phosphoric acid
Potassium dihydrogen 0.5g/L.
Wherein, fermentation medium composition is:Glucose 10g/L, sucrose 23g/L, yeast extract 6g/L, peptone 9g/L.
The 5th day, 7 days and 9 days is being fermented to gained cordyceps sinensis oatmeal sampling analysis respectively, the results showed that:As cordyceps sinensis is trained
The foster time is elongated, and cordyceps sinensis active component substantially increases in culture, but obtained cordyceps sinensis oat culture yield declines, as a result such as
Shown in table 1.
Table 1:Cordyceps Militaris single bacterium fermented oats piece result
Embodiment 2
A kind of selenium-rich cordyceps oat steamed bun is present embodiments provided, is prepared by the following method:
The preparation of cordyceps sinensis oatmeal:It is that the oatmeal 500g that Australian import oat making is bought from market loads fermentation
Container, in temperature, 123 degree sterilize 30 minutes, access 100 milliliters of the cordyceps sinensis seed culture fluid cultivated in advance, add sterilized water 125
Milliliter, in temperature 23-25 degree after stirring, relative humidity is that 80-85% is cultivated 2.5 days, and after forming mycelia, oat is cultivated
Thing stirs stirring once up and down, continues culture 3.5 days, oat culture is then obtained cordyceps sinensis oat in 80-85 degree drying and crushings
Powder 452.7g.(cordyceps sinensis oatmeal adenosine containing cordyceps sinensis 1.97%, cordycepin 0.013%)
Wherein, the cordyceps sinensis that the present invention uses is aweto J3, is separated from the fresh cordyceps sinensis of Nagqu, and
Determined through morphological feature and DNA sequencing.
Wherein, the preparation for the cordyceps sinensis seed culture fluid that the present invention uses:It is that slant strains access is equipped with seed culture medium
Triangular flask in, be 160-200 revs/min in rotary speed, cultivation temperature is 25 degree and lower cultivated 3 days.Seed culture medium forms:
200 g/l of potato, 16 g/l of glucose, 7 g/l of peptone, PH are natural.
The culture and collection of Se-enriched yeast mud:The S. cervisiae that will be tamed on the slant medium containing selenium, is inoculated into
Liquid seed culture medium, under the conditions of 160-200 revs/min of rotary speed, temperature 28-32 degree, 18-20 hours are cultivated, are planted
Sub- liquid.Fermentation medium is transferred to by 5% grain weight of fermentation medium volume ratio, in 180 revs/min of rotary speed, temperature 28-
Ventilate and cultivate under the conditions of 32 degree, and the selenium solution to sterilize in advance is added in growth late log phase, make the selenium concentration of fermentation medium
Reach 10ug/ml, continue culture under these conditions by 40 hours, then centrifuged 15 minutes at 5000 revs/min, incline supernatant
Liquid, the yeast paste of active selenium-rich is obtained, (yeast selenium 24.3ug/g), it is standby to preserve refrigerator.
Wherein, yeast starter culture medium composition is:Glucose 10g/L, yeast extract 5g/L, peptone 6g/L, PH5.0-
5.5。
Wherein, yeast fermentation medium composition is:Glucose 10g/L, yeast extract 5g/L, peptone 6g/L, PH5.0-
5.5。
The making of steamed bun:By different proportion, cordyceps sinensis oatmeal and wholewheat flour, sucrose 25g, milk powder 20g, low are weighed respectively
Glycan 10g is poured into round, takes selenium-enriched active yeast paste 2g, is separately added into the warm water of flour total amount different proportion, stirring
It is well mixed, add into round, stirring rubs up into dough, soft tack-free for degree with dough.In relative humidity
75%th, temperature 30-35 degree is waken up 4 hours of face, when reaching more than 2 times of former dough volume to dough fermentation volume, will be fermented
Dough be divided into 50 grams/piece of dough/pasta, then proof 20 minutes, when steamer emerges steam, good wake flour rolled into a ball into upper cage vapour
Steam, obtain selenium-rich cordyceps oat for health care steamed bun.
Compared with common wheat steamed bun, the water consumption with the increase of cordyceps sinensis oatmeal addition, and face increases therewith, when
In mixed flour, the ratio of cordyceps sinensis oatmeal from 20% bring up to more than 80% when, and face water consumption ratio brings up to from 55%
More than 105%.When making steamed bun amount of water less than 55%, dough fermentation enlarged volume is less obvious, dough internal cellular network
Few, the steamed bun steamed hardens a little, sticks to one's teeth, without elasticity.When amount of water is more than 105%, dough surface glues hand, the steamed bun steamed
Ftracture on top.
Embodiment 3
A kind of selenium-rich cordyceps oat steamed bun is present embodiments provided, is prepared by the following raw material:Cordyceps sinensis oatmeal 300g,
Wholewheat flour 200g, Se-enriched yeast mud 2.5g, sucrose 30g, milk powder 20g, oligosaccharide 15g.
Specifically, it is prepared by the following method:
The preparation of cordyceps sinensis oatmeal:It is that the oatmeal 500g that Australian import oat making is bought from market loads fermentation
Container, in temperature, 123 degree sterilize 30 minutes, access 100 milliliters of the cordyceps sinensis seed culture fluid cultivated in advance, add sterilized water 125
Milliliter, in temperature 23-25 degree after stirring, relative humidity is that 80-85% is cultivated 2.5 days, and after forming mycelia, oat is cultivated
Thing stirs stirring once up and down, continues culture 3.5 days, oat culture is then obtained cordyceps sinensis oat in 80-85 degree drying and crushings
Powder 452.7g.(cordyceps sinensis oatmeal adenosine containing cordyceps sinensis 0.091%, cordycepin 0.613%)
Wherein, the cordyceps sinensis that the present invention uses is Cordyceps militaris, is given by Shanghai East China University of Science and Technology.
Wherein, the preparation for the cordyceps sinensis seed culture fluid that the present invention uses:It is that slant strains access is equipped with seed culture medium
Triangular flask in, be 160-200 revs/min in rotary speed, cultivation temperature is 25 degree and lower cultivated 3 days.Seed culture medium forms:
200 g/l of potato, 16 g/l of glucose, 7 g/l of peptone, PH are natural.
The culture of Se-enriched yeast mud and collection:The S. cervisiae that will be tamed on the slant medium containing selenium, is inoculated into liquid
Body seed culture medium, under the conditions of 160-200 revs/min of rotary speed, temperature 28-32 degree, 18-20 hours are cultivated, make seed
Liquid.Fermentation medium is transferred to by 5% grain weight of fermentation medium volume ratio, in 180 revs/min of rotary speed, temperature 28-32
Ventilate and cultivate under the conditions of degree, and the selenium solution to sterilize in advance is added in growth late log phase, reach the selenium concentration of fermentation medium
To 10ug/ml, continue culture under these conditions by 40 hours, then centrifuged 15 minutes at 5000 revs/min, incline supernatant
Liquid, the yeast paste of active selenium-rich is obtained, (yeast selenium 25.5ug/g), it is standby to preserve refrigerator.
Wherein, yeast starter culture medium composition is:Glucose 10g/L, yeast extract 5g/L, peptone 6g/L, PH5.0-
5.5。
Wherein, yeast fermentation medium composition is:Glucose 10g/L, yeast extract 5g/L, peptone 6g/L, PH5.0-
5.5。
The making of steamed bun:Insect-taking grass oatmeal 400g, wholewheat flour 100g, sucrose 25g, milk powder 20g, oligosaccharide 10g fall
Enter in stirring container, take Se-enriched yeast mud 3g to add 513ml water, be uniformly mixed, pour into stirring container, stirring rubs up into
Dough, waken up 4 hours of face in relative humidity 75%, temperature 30-35 degree, 2 times of former dough volume are reached to dough fermentation volume
During the above, the dough fermented is divided into 50 grams/piece of dough/pasta, then proofed 20 minutes, when steamer emerges steam, will be waken up
Cage decatize on good dough, obtains selenium-rich cordyceps oat for health care steamed bun.
The steamed bun steamed and common wheat steamed bun are basically identical, have cordyceps sinensis oat smell and milk fragrance, rich in Organic Selenium
With cordyceps sinensis active component.
Embodiment 4
A kind of selenium-rich cordyceps oat steamed bun is present embodiments provided, is prepared by the following raw material:Cordyceps sinensis oatmeal 300g,
Wholewheat flour 200g, Se-enriched yeast mud 2.5g, sucrose 30g, milk powder 20g, oligosaccharide 15g.
Specifically, it is prepared by the following method:
The preparation of cordyceps sinensis oatmeal:It is that the oatmeal 500g that Australian import oat making is bought from market loads fermentation
Container, in temperature, 123 degree sterilize 45 minutes, access 100 milliliters of the cordyceps sinensis seed culture fluid cultivated in advance, add the milli of sterilized water 80
Rise, in temperature 23-25 degree after stirring, relative humidity is 70% culture 2.5 days, after forming mycelia, on oat culture
Under stir stirring once, continue culture 2.5 days, oat culture is then obtained cordyceps sinensis oatmeal in 80 degree of drying and crushings
474.3g.(every gram of cordyceps sinensis oatmeal adenosine containing cordyceps sinensis 1.53%, cordycepin 0.010%)
Wherein, the cordyceps sinensis that the present invention uses is aweto J3, is separated from the fresh cordyceps sinensis of Nagqu, and
Is determined through morphological feature and DNA sequencing.
Wherein, the preparation for the cordyceps sinensis seed culture fluid that the present invention uses:It is that slant strains access is equipped with seed culture medium
Triangular flask in, be 200 revs/min in rotary speed, cultivation temperature is 23 degree and lower cultivated 2 days.
Wherein, seed culture medium forms:200 g/l of potato, 16 g/l of glucose, 7 g/l of peptone, PH are natural.
The culture and collection of Se-enriched yeast mud:The S. cervisiae that will be tamed on the slant medium containing selenium, is inoculated into
In liquid seed culture medium, cultivated 18 hours under the conditions of 180 revs/min of rotary speed, temperature 30-32 degree, make seed liquor.Press
Culture volume than 5% grain weight be transferred in fermentation medium, in 180 revs/min of rotary speed, temperature 30-32 degree conditions
Lower ventilation culture, and the selenium solution to sterilize in advance is added in growth late log phase, the selenium concentration of fermentation medium is reached 10ug/
Ml, continue culture under these conditions by 40 hours, then centrifuged 15 minutes at 6000 revs/min, incline supernatant, is lived
Property selenium-rich yeast paste, (yeast selenium 25.8ug/g), preserve refrigerator it is standby.
Wherein, yeast starter culture medium composition is:Glucose 10g/L, yeast extract 5g/L, peptone 6g/L, PH5.0-
5.5。
Wherein, yeast fermentation medium composition is:Glucose 10g/L, yeast extract 5g/L, peptone 6g/L, PH5.0-
5.5。
The making of steamed bun:Insect-taking grass oatmeal 300g, wholewheat flour 200g, sucrose 30g, milk powder 20g, oligosaccharide 15g fall
Enter in stirring container, take Se-enriched yeast mud 2.5g to add the water of 35 degree of 445ml temperature, be uniformly mixed, pour into stirring container,
Stirring rubs up into dough, and in relative humidity 75%, temperature 32-33 degree is waken up 4 hours of face, reaches former dough to dough fermentation volume
At more than 2 times of volume, the dough fermented is divided into 50 grams/piece of dough/pasta, proofed 20 minutes, when steamer is emerged steaming
During vapour, good wake flour is rolled into a ball into upper cage decatize, obtains selenium-rich cordyceps oat for health care steamed bun.
The steamed bun steamed and common wheat steamed bun are basically identical, have cordyceps sinensis oat smell and milk fragrance, rich in Organic Selenium
With cordyceps sinensis active component,
Embodiment 5
A kind of selenium-rich cordyceps oat steamed bun is present embodiments provided, is prepared by the following raw material:Cordyceps sinensis oatmeal 200g,
Wholewheat flour 300g, Se-enriched yeast mud 3g, sucrose 30g, milk powder 20g, oligosaccharide 15g.
Specifically, it is prepared by the following method:
The preparation of cordyceps sinensis oatmeal:It is that the oatmeal 250g that Australian import oat making is bought from market loads fermentation
Container, in temperature, 123 degree sterilize 40 minutes, access 50 milliliters of the cordyceps sinensis seed culture fluid cultivated in advance, add the milli of sterilized water 60
Rise, in temperature 23-25 degree after stirring, relative humidity is 70% culture 2.5 days, after forming mycelia, on oat culture
Under stir stirring once, continue culture 3 days, oat culture is then obtained cordyceps sinensis oatmeal 240.1g in 80 degree of drying and crushings.
(every gram of cordyceps sinensis oatmeal adenosine containing cordyceps sinensis 0.101%, cordycepin 0.727%).
Wherein, the cordyceps sinensis that the present invention uses is Cordyceps militaris, is given by Shanghai East China University of Science and Technology.
Wherein, the preparation for the cordyceps sinensis seed culture fluid that the present invention uses:It is that slant strains access is equipped with seed culture medium
Triangular flask in, be 200 revs/min in rotary speed, cultivation temperature is 23 degree and lower cultivated 2 days.
Wherein, seed culture medium forms:200 g/l of potato, 16 g/l of glucose, 7 g/l of peptone, PH are natural.
The culture and collection of Se-enriched yeast mud:The S. cervisiae that will be tamed on the slant medium containing selenium, is inoculated into
In liquid seed culture medium, cultivated 18 hours under the conditions of 180 revs/min of rotary speed, temperature 30-32 degree, make seed liquor.Press
Culture volume than 5% grain weight be transferred to fermentation medium, under the conditions of 180 revs/min of rotary speed, temperature 30-32 degree
Ventilation culture, and the selenium solution to sterilize in advance is added in growth late log phase, the selenium concentration of fermentation medium is reached 10ug/
Ml, continue culture under these conditions by 40 hours, then centrifuged 15 minutes at 6000 revs/min, incline supernatant, is lived
Property selenium-rich yeast paste, (yeast selenium 24.7ug/g), preserve refrigerator it is standby.
Wherein, yeast starter culture medium composition is:Glucose 10g/L, yeast extract 5g/L, peptone 6g/L, PH5.0-
5.5。
Wherein, yeast fermentation medium composition is:Glucose 10g/L, yeast extract 5g/L, peptone 6g/L, PH5.0-
5.5。
The making of steamed bun:Insect-taking grass oatmeal 200g, sucrose 30g, milk powder 20g, oligosaccharide 15g are poured into round,
Take Se-enriched yeast mud 3g to add the water of 38 degree of 372ml temperature, be uniformly mixed, pour into round, add whole wheat face
Powder 300g, stirring rub up into dough, in relative humidity 75%, temperature 32-33 degree fermentation 4 hours of face, to dough fermentation volume
When reaching more than 2 times of former dough volume, the dough fermented is divided into 50 grams/piece of dough/pasta, proofed 20 minutes, when
Steamer emerge steam when, good wake flour is rolled into a ball into upper cage decatize, obtains selenium-rich cordyceps oat for health care steamed bun.
The steamed bun steamed and common wheat steamed bun are basically identical, have cordyceps sinensis oat smell and milk fragrance, rich in Organic Selenium
With cordyceps sinensis active component,
Embodiment 6
A kind of selenium-rich cordyceps oat steamed bun is present embodiments provided, is prepared by the following raw material:Cordyceps sinensis oatmeal 100g,
Wholewheat flour 400g, Se-enriched yeast mud 2g, sucrose 25g, milk powder 20g, oligosaccharide 15g.
Specifically, it is prepared by the following method:
The preparation of worm summer oatmeal:It is the oatmeal that Australian import making is bought from market, loads round,
123 degree of sterilizing 30-45 minutes, by oat sheet weight than accessing the cordyceps sinensis seed culture fluid cultivated in advance for 30% grain weight,
In 25 degree of temperature after stirring, relative humidity is that 65-85% is cultivated 2.5 days, and after forming mycelia, oat culture is turned over up and down
Dynamic stirring once, continues culture 4 days, oat culture is then obtained cordyceps sinensis oatmeal in 80-100 degree drying and crushings, (every gram of worm
Careless oatmeal adenosine containing cordyceps sinensis 1.77%, cordycepin 0.013%)
Wherein, the cordyceps sinensis that the present invention uses is aweto J3, is separated from the fresh cordyceps sinensis of Nagqu, and
Determined through morphological feature and DNA sequencing.
Wherein, the preparation for the cordyceps sinensis seed culture fluid that the present invention uses:It is that slant strains access is equipped with seed culture medium
Triangular flask in, be 160-200 revs/min in rotary speed, cultivation temperature is 25 degree and lower cultivated 2-3 days.Seed culture medium group
Into:200 g/l of potato, 16 g/l of glucose, 7 g/l of peptone, PH are natural.
The culture and collection of Se-enriched yeast mud:The S. cervisiae that will be tamed on the slant medium containing selenium, is inoculated into
In liquid seed culture medium, 18-20 hours are cultivated under the conditions of 160-200 revs/min of rotary speed, temperature 28-32 degree, are planted
Sub- liquid.By culture volume than 5-10% grain weights be transferred in fermentation medium, in 160-200 revs/min of rotary speed, temperature
Ventilation is cultivated under the conditions of spending 28-32 degree, and the selenium solution to sterilize in advance is added in growth late log phase, makes the selenium of fermentation medium
Concentration reaches 10ug/ml, continues culture under these conditions and arrives 38-40 hours, then centrifuges 10- at 5000-8000 revs/min
15 minutes, the supernatant that inclines obtained the yeast paste of active selenium-rich, (yeast selenium 26.1ug/g), it is standby to preserve refrigerator.
Wherein, yeast starter culture medium composition is:Glucose 10g/L, yeast extract 5g/L, peptone 6g/L, PH5.0-
5.5。
Wherein, yeast fermentation medium composition is:Glucose 10g/L, yeast extract 5g/L, peptone 6g/L, PH5.0-
5.5。
The making of steamed bun:Insect-taking grass oatmeal 100g, sucrose 25g, milk powder 20g, oligosaccharide 15g;Take Se-enriched yeast mud
2g, the water of 35 degree of 296ml temperature is added, is uniformly mixed, pours into round, add wholewheat flour 400g, stirring is rubbed
With into dough, in relative humidity 75%, 35 degree of temperature is waken up 3 hours of face, and the dough proofed is divided into 50 grams/piece of dough
Group, is proofing 15 minutes, when steamer emerges steam, good wake flour is rolled into a ball into upper cage decatize, obtains selenium-rich cordyceps oat for health care steamed bun
Head.
The steamed bun steamed and common wheat steamed bun are basically identical, have cordyceps sinensis oat smell and milk fragrance, rich in Organic Selenium
With cordyceps sinensis active component,
Embodiment 7
A kind of selenium-rich cordyceps oat steamed bun is present embodiments provided, is prepared by the following raw material:Chinese caterpillar fungus fermentation magma
200g, oatmeal 400g, wholewheat flour 100g, Se-enriched yeast powder 0.5g, active dry yeast 1.5g, sucrose 25g, milk powder 20g are low
Glycan 8g.
Specifically, it is prepared by the following method:
The preparation of Chinese caterpillar fungus fermentation magma:Cordyceps sinensis inclined-plane seed is inoculated into seed culture medium, 200 turns of rotary speed/
Minute, under the conditions of temperature 23-24 degree, culture obtains seed liquor in 2 days, is then connect by the grain weight that fermentation medium volume ratio is 10%
Enter in fermentation medium, cultivated 3 days under the conditions of 200 revs/min of rotary speed, temperature 23-24 degree, obtain Chinese caterpillar fungus fermentation magma
(cordyceps sinensis adenosine 1.61mg/100ml, cordycepin 0.019mg/100ml).
Wherein, the cordyceps sinensis that the present invention uses is aweto J3, is separated from the fresh cordyceps sinensis of Nagqu, and
Determined through morphological feature and DNA sequencing.
Wherein, seed culture medium composition is:Glucose 5g/L, sucrose 15g/L, yeast extract 3g/L, peptone 6g/L, phosphoric acid
Potassium dihydrogen 0.5g/L.
Wherein, fermentation medium composition is:Glucose 10g/L, sucrose 23g/L, yeast extract 6g/L, peptone 9g/L.
The preparation of Se-enriched yeast powder:The S. cervisiae that will be tamed on the slant medium containing selenium, is inoculated into liquid strain
Sub- culture medium, 18-20 hours are cultivated under the conditions of 160 revs/min of rotary speed, temperature 28-32 degree, make seed liquor.By fermentation
Culture volume than 6% grain weight be transferred in fermentation medium, in 160 revs/min of rotary speed, temperature 30-32 degree conditions
Lower ventilation culture, and the selenium solution to sterilize in advance is added in growth late log phase, the selenium concentration of fermentation medium is reached 10ug/
Ml, continue culture under these conditions by 42 hours, then centrifuged 15 minutes at 6000 revs/min, the supernatant that inclines is lived
Property selenium-enriched active yeast paste, obtain selenium-rich dried yeast powder in 85 degree of drying and crushings of temperature, (dusty yeast selenium 149.6ug/g) preserve it is standby
With.
Wherein, seed culture medium composition is:Glucose 5g/L, sucrose 15g/L, yeast extract 3g/L, peptone 6g/L, phosphoric acid
Potassium dihydrogen 0.5g/L.
Wherein, fermentation medium composition is:Glucose 10g/L, sucrose 23g/L, yeast extract 6g/L, peptone 9g/L.
The making of steamed bun:Oatmeal 400g is bought in market, adds sucrose 25g, Se-enriched yeast powder 0.5g, milk powder 20g, oligomeric
Sugared 8g, is poured into round, and (the Chinese caterpillar fungus fermentation magma, which adds, lives by insect-taking grass fermentation magma 200ml, active dry yeast 1.5g
Property dry ferment be modulated into pasty state, can be as the leavening that dough fermentation proofs), add the water of 32 degree of 311ml temperature, be stirred
Uniformly, into stirring container, wholewheat flour 100g is added, stirring rubs up into dough, in relative humidity 75%, temperature 32-33 degree
Fermentation 4.1 hours, when reaching more than 2 times of former dough volume to dough fermentation volume, by the dough fermented be divided into 50 grams/
Individual dough/pasta, proofing 20 minutes, when steamer emerges steam, good wake flour is being rolled into a ball into upper cage decatize, obtains selenium-rich cordyceps swallow
Wheat steamed bun.
The steamed bun steamed and common wheat steamed bun are basically identical, have cordyceps sinensis oat smell and milk fragrance, rich in Organic Selenium
With cordyceps sinensis active component.
Although above the present invention is made to retouch in detail with general explanation, embodiment and experiment
State, but on the basis of the present invention, it can be made some modifications or improvements, this is apparent to those skilled in the art
's.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, are belonged to claimed
Scope.
Claims (10)
1. a kind of selenium-rich cordyceps oat steamed bun, it is characterised in that in parts by weight, by the raw material preparation including following composition
Into:100~450 parts of cordyceps sinensis oatmeal, 50~400 parts of wholewheat flour, 1.0~3.5 parts of Se-enriched yeast mud, 10~20 parts of milk powder,
15~25 parts of sucrose, 7~15 parts of oligosaccharide;
The cordyceps sinensis oatmeal is after mixing cordyceps sinensis seed liquor and oatmeal, culture, to dry, crush and obtain.
2. selenium-rich cordyceps oat steamed bun according to claim 1, it is characterised in that the cordyceps sinensis seed liquor is to train inclined-plane
Support on base in the cordyceps cultivated or aweto access liquid seed culture medium, rotating speed be 160~200 revs/min,
Temperature obtains to cultivate 2~3 days under the conditions of 23~25 DEG C;
Preferably, contain in the seed culture medium:150~250g/L of potato, 10~20g/L of glucose, peptone 5~
10g/L。
3. selenium-rich cordyceps oat steamed bun according to claim 1 or 2, it is characterised in that the cordyceps sinensis oatmeal by including
The method of following steps is prepared:Oatmeal after sterilizing is accessed into cordyceps sinensis seed liquor, while adds sterilized water, is stirred
Cultivated 2~3 days under conditions of 23~25 DEG C, relative humidity 80~85% afterwards, after stirring stirring up and down, continue culture 3~4
My god, dry, crush, produce;
Preferably, the access cordyceps sinensis seed liquor quality accounts for the 10~30% of the oat tablet quality.
4. selenium-rich cordyceps oat steamed bun according to claim 1, it is characterised in that the Se-enriched yeast mud is will be oblique containing selenium
The S. cervisiae cultivated on the culture medium of face is inoculated into liquid yeast seed culture medium, 160~200 revs/min of rotating speed, 28~
Cultivated 18~20 hours under the conditions of 32 DEG C, obtain seed liquor;The seed liquor is accessed into liquid yeast fermentation medium, in rotating speed
160~200 revs/min, culture of ventilating under the conditions of 28~32 DEG C of temperature, and the selenium solution of sterilizing is added in growth late log phase,
The selenium element concentration in the fermentation medium is reached 5~15ug/ml, continue culture by 36~42 hours, gone after centrifugation
Clearly, Se-enriched yeast mud is obtained;
Preferably, the liquid yeast seed culture medium composition is:1~10g/L of glucose, 1~10g/L of yeast extract, peptone 1
~10g/L, pH5.0~5.5;And/or the liquid yeast fermentation medium composition is:10~20g/L of glucose, yeast extract 1
~10g/L, 1~10g/L of peptone, pH5.0~5.5.
5. the preparation method of selenium-rich cordyceps oat steamed bun described in Claims 1 to 4 any one, it is characterised in that including as follows
Step:Se-enriched yeast mud is mixed with water, other raw materials is added and is sufficiently mixed, stirring rubs up into dough, is divided into after fermentation
Type, proof, steam, produce;
Preferably, the fermentation is 3~5 hours of being fermented under the conditions of relative humidity 70~80%, 30~35 DEG C of temperature.
6. a kind of selenium-rich cordyceps oat steamed bun, it is characterised in that be prepared by the raw material including following parts by weight meter:Cordyceps sinensis sends out
150~200 parts of ferment magma, 100~400 parts of oatmeal, 100~400 parts of wholewheat flour, 0.5~1.0 part of Se-enriched yeast powder are living
1.0~3.5 parts of dry ferment of property, 10~20 parts of milk powder, 15~25 parts of sucrose, 7~15 parts of oligosaccharide.
7. selenium-rich cordyceps oat steamed bun according to claim 6, it is characterised in that the Chinese caterpillar fungus fermentation magma is by inclined-plane
It it is 160~200 revs/min in rotating speed in cordyceps or aweto the access liquid seed culture medium cultivated on culture medium
Cultivated 2~3 days under the conditions of clock, 23~25 DEG C of temperature, obtain seed liquor;The seed liquor is accessed in liquid fermentation medium,
Cultivated 4~5 days under the conditions of rotating speed is 160~200 revs/min, temperature is 23~25 DEG C, obtain Chinese caterpillar fungus fermentation magma;
Preferably, contain in the seed culture medium:1~10g/L of glucose, 10~50g/L of sucrose, 1~5g/L of yeast extract, egg
1~10g/L of white peptone, 0.1~1g/L of potassium dihydrogen phosphate;And/or contain in the fermentation medium:5~15g/L of glucose, sugarcane
20~30g/L of sugar, 1~10g/L of yeast extract, 1~10g/L of peptone.
8. selenium-rich cordyceps oat steamed bun according to claim 6, it is characterised in that the Se-enriched yeast powder is will be oblique containing selenium
The S. cervisiae cultivated on the culture medium of face is inoculated into liquid yeast seed culture medium, 160~200 revs/min of rotating speed, 28~
Cultivated 18~20 hours under the conditions of 32 DEG C, obtain seed liquor;The seed liquor is accessed into liquid yeast fermentation medium, in rotating speed
160~200 revs/min, culture of ventilating under the conditions of 28~32 DEG C of temperature, and the selenium solution of sterilizing is added in growth late log phase,
The selenium element concentration in the fermentation medium is reached 5~15ug/ml, continue culture by 36~42 hours, gone after centrifugation
Clearly, Se-enriched yeast mud is obtained;After Se-enriched yeast mud drying, crushing, Se-enriched yeast powder is obtained;
Preferably, the liquid yeast seed culture medium composition is:1~10g/L of glucose, 1~10g/L of yeast extract, peptone 1
~10g/L, pH5.0~5.5;And/or the liquid yeast fermentation medium composition is:10~20g/L of glucose, yeast extract 1
~10g/L, 1~10g/L of peptone, pH5.0~5.5.
9. the preparation method of selenium-rich cordyceps oat steamed bun described in claim 6~8 any one, it is characterised in that including as follows
Step:Chinese caterpillar fungus fermentation magma and active dry yeast are mixed to get leavening;After the leavening is mixed with water, it is added
Its raw material is sufficiently mixed, and stirring rubs up into dough, and shaping is split after fermentation, proofs, steams, produce.
10. according to the method for claim 9, it is characterised in that the fermentation is in relative humidity 70~80%, temperature 30
Fermented under the conditions of~35 DEG C 3~5 hours.
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CN108029955A (en) * | 2017-12-29 | 2018-05-15 | 黄河三角洲京博化工研究院有限公司 | A kind of selenium-rich steamed bun and preparation method thereof |
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CN104286690A (en) * | 2014-11-04 | 2015-01-21 | 郑州职业技术学院 | Steamed oatmeal buns and processing method thereof |
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CN102823806A (en) * | 2012-09-20 | 2012-12-19 | 王永显 | Method for producing fermented oatmeal using Cordyceps militaris |
CN104286690A (en) * | 2014-11-04 | 2015-01-21 | 郑州职业技术学院 | Steamed oatmeal buns and processing method thereof |
CN105124398A (en) * | 2015-07-15 | 2015-12-09 | 湖北省农业科学院农产品加工与核农技术研究所 | Production method of functional frozen dough |
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