CN106434492A - Culture medium for effectively prolonging survival time of lactic acid bacteria and preparation method of culture medium - Google Patents
Culture medium for effectively prolonging survival time of lactic acid bacteria and preparation method of culture medium Download PDFInfo
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Abstract
The invention discloses a culture medium for effectively prolonging the survival time of lactic acid bacteria and a preparation method of the culture medium. The culture medium for effectively prolonging the survival time of the lactic acid bacteria is prepared by taking peptone, yeast extract powder, mannan oligosaccharide, skim milk powder, defatted soybean meal, magnesium sulfate heptahydrate, manganese sulfate heptahydrate, dried corn steep liquor powder, a compound additive, sodium dihydrogen phosphate, sodium citrate, citric acid, sodium acetate, Tween-80 and saccharomyces cerevisiae as raw materials; in addition, the compound additive is a mixture of sodium carboxymethylcellulose, sodium alginate, bone meal, royal jelly acid, guar gum and xanthan gum. According to the culture medium for effectively prolonging the survival time of the lactic acid bacteria, the functions of all the raw materials are mutually cooperative, and higher variable bacteria quantity of the lactic acid bacteria can be kept at normal temperature for a long time; in addition, the culture medium has the advantages of simplicity and convenience in operation, easily-obtained raw materials, low cost and high homogenization degree of a product; the lactic acid bacteria is enabled to be more stable in property and higher in biological activity and can be stored for a longer time.
Description
Technical field
The present invention relates to a kind of effective culture medium for extending lactic acid bacteria survival period and preparation method thereof.
Background technology
Since the important function of lactic acid bacteria being found from Russian biologist Mei Qinikefu, lactic acid bacteria and lactobacillus beverage exist
The application of countries in the world is quite popularized, and people are more and more deep to its understanding.A large amount of animal and clinical trial both at home and abroad
Prove, lactic acid bacteria has preferable health care and therapeutic efficiency to humans and animals, and its Main Function has：Improve gastrointestinal function, dimension
Hold intestinal microbial population balance.Lactic acid bacteria is intestinal often in bacterium, thus it is possible to vary environment in animal intestinal, suppression harmful bacteria breeding, adjustment
Gastrointestinal bacterial flora is balanced.After poultry take lactic acid bacteria, lactic acid bacteria is combined closely with protection of intestinal mucosal barrier cells by adhesin, in intestinal mucosa
Surface is colonized occupy-place, becomes the key component of physiologic barrier, so as to reach recovery host resistance, repairs intestinal microbial population screen
Barrier, the effect of healing intestinal tract disease.If this barrier is destroyed by antibiotic or other factors, host is lost to external
Bacterium resistance, can make the intestinal flora abnormality proliferation with drug resistance and replace the position of dominant bacteria, cause Tiny ecosystem in intestinal to put down
The imbalance of weighing apparatus.Secondly, lactic acid bacteria can provide nutrient substance for body, promote body growth, and lactic acid bacteria directly can be carried for host
For available essential amino acids and various vitamin, the biological activity of mineral element can be also improved, and then be reached for host carry
For required nutriment, strengthen the Nutrition and Metabolism of animal, directly promote the effect of its growth, it is also possible to strengthening animal body to nutrition
The absorption of material.Again, lactic acid bacteria has the effect of enhance immunity, and the on the one hand phagocytosis of the obvious activating macrophage of energy is made
With on the other hand as it can be in intestinal colonisation, equivalent to natural active immunity, moreover it is possible to stimulate peritoneal macrophageses, induction to produce
Raw interferon, promote cell division, produce antibody and promote cellular immunization etc., so can the non-specificity of enhancing body and special
Property immunoreation, improve body resistances against diseases.Finally, lactic acid bacteria has good suppression to make to some pyschrophiles and putrefaction bacteria
With can be used to prevent and treat diarrhoea, dysentery, enteritis, constipation and the multiple diseases for causing due to function of intestinal canal disorder and scytitiss.
To sum up, lactic acid bacteria plays very important effect to the health of animal body, but lactic acid bacteria will in animal intestinal
Give full play to its effect, it is necessary to assure lactic acid bacteria is active bacteria.And presently commercially available lactic acid bacteria product shelf lives are short, under room temperature
Viable bacteria amount declines rapidly, and animal can not receive good effect after drinking, it is impossible to meet demand of the people to liquid lactic acid bacterium.And show
Have in technology, in order to avoid these problems, often extend the survival period of viable lactic acid bacteria using culture medium, improve lactic acid bacteria
Biological activity.Culture medium is the artificial preparation nutriment for growing for microorganism, plant tissue and animal tissue and maintaining, and typically contains
Have carbohydrate, nitrogen substance, inorganic salt, vitamin and water etc., have also containing antibiotics, pigment, hormone and serum.And
The existing effect for extending the culture medium of viable lactic acid bacteria survival period is not satisfactory, far can not meet the demand of people.
So, need a kind of culture medium that can effectively extend lactic acid bacteria survival period badly.
Content of the invention
The present invention is in order to make up the deficiencies in the prior art, there is provided a kind of easy to operate, with low cost, lactic acid bacteria can be made
Property is more stable, biological activity is higher, store more permanent culture medium of effective prolongation lactic acid bacteria survival period and preparation method thereof.
The present invention is achieved through the following technical solutions：
A kind of effective culture medium for extending lactic acid bacteria survival period, its active component is made up of the raw material of following parts by weight：
Peptone 0.08-0.12 part, yeast extract 0.08-0.12 part, mannooligo saccharide 1.5-2.5 part, defatted milk powder 0.16-0.24 part,
Defatted soybean meal 0.5-0.7 part, Magnesium sulfate heptahydrate 0.05-0.07 part, seven water manganese sulfate 0.025-0.035 parts, Dried Corn Steep Liquor Powder
1.5-2.5 part, compound additive 0.008-0.012 part, sodium dihydrogen phosphate 0.025-0.035 part, sodium citrate 0.25-0.35
Part, citric acid 0.2-0.3 part, sodium acetate 0.08-0.12 part, Tween 80 are 0.08-0.12 part, saccharomyces cerevisiae 0.04-0.06 part；
The compound additive is the mixture of sodium carboxymethyl cellulose, sodium alginate, bone meal, Royal Jelly acid, guar gum and xanthan gum,
And mass ratio is sodium carboxymethyl cellulose：Sodium alginate：Bone meal：Royal Jelly acid：Guar gum：Xanthan gum=2：2：3：1：1：2；Institute
The colony density for stating saccharomyces cerevisiae is 2 × 105cfu/g.
Preferably, the parts by weight of each active component are：0.1 part of peptone, 0.1 part of yeast extract, 2 parts of mannooligo saccharide,
0.2 part of defatted milk powder, 0.6 part of defatted soybean meal, 0.06 part of Magnesium sulfate heptahydrate, 0.03 part of seven water manganese sulfate, 2 parts of Dried Corn Steep Liquor Powder,
0.01 part of compound additive, 0.03 part of sodium dihydrogen phosphate, 0.3 part of sodium citrate, 0.25 part of citric acid, 0.1 part of sodium acetate, tween
80 is 0.1 part, 0.05 part of saccharomyces cerevisiae；The compound additive be sodium carboxymethyl cellulose, sodium alginate, bone meal, Royal Jelly acid,
Guar gum and the mixture of xanthan gum, and mass ratio is sodium carboxymethyl cellulose：Sodium alginate：Bone meal：Royal Jelly acid：Guar beanss
Glue：Xanthan gum=2：2：3：1：1：2；The colony density of the saccharomyces cerevisiae is 2 × 105cfu/g.
The preparation method of above-mentioned effective culture medium for extending lactic acid bacteria survival period, comprises the steps：
(1) Dried Corn Steep Liquor Powder is placed in agitator tank, adds 8 DEG C -12 DEG C of 4-6 times of Dried Corn Steep Liquor Powder quality of purification
Water, stirs into pasty state, after placing 20-30 minute, adds 15 DEG C -20 DEG C of 2-4 times of Dried Corn Steep Liquor Powder quality of purified water, stirring
Uniformly；
(2) successively by peptone, yeast extract, mannooligo saccharide, defatted milk powder, defatted soybean meal, Magnesium sulfate heptahydrate, seven water sulfur
Sour manganese, step (1) products therefrom, sodium dihydrogen phosphate, sodium citrate, citric acid, sodium acetate, Tween 80 and saccharomyces cerevisiae are put into burning
15 DEG C -18 DEG C of 0.6-0.8 times of mixture quality of purified water in cup, is added, is stirred, obtain mixed liquor；
(3) by the sodium carboxymethyl cellulose in compound additive, sodium alginate, bone meal, Royal Jelly acid, guar gum and xanthan
Glue is 2 in mass ratio：2：3：1：1：2 ratio mix homogeneously, is added in the pure water of 80-100 times of compound additive quality,
Dissolving is fully mixed；
(4) products therefrom in step (3) is added in step (2) in the beaker for filling in mixed liquor, mix homogeneously, will
The beaker for filling mixed material after mix homogeneously is placed in and is covered with the electric furnace of asbestos gauge, and small fire is heated, the glass in heating
Rod is stirred 25-35 minute clockwise, stops heating；
(5) toward in step (4) products therefrom, add mass concentration for the HCl solution of 5%-7%, regulation pH value is 5.4-
5.8；
(6) by step (5) products therefrom autoclaving 18-22 minute, effective training for extending lactic acid bacteria survival period is obtained final product
Foster base.
The compound additive is sodium carboxymethyl cellulose, sodium alginate, bone meal, Royal Jelly acid, guar gum and xanthan gum
Mixture, and mass ratio be sodium carboxymethyl cellulose：Sodium alginate：Bone meal：Royal Jelly acid：Guar gum：Xanthan gum=2：2：
3：1：1：2.
The colony density of the saccharomyces cerevisiae is 2 × 105Cfu/g, is that saccharomyces cerevisiae is placed in saccharomyces cerevisiae liquid culture
Gained is cultivated in base, and the wherein composition of saccharomyces cerevisiae fluid medium is 0.5 part of peptone, 1 part of mannooligo saccharide, yeast extract
0.3 part, 0.3 part of beerwort.
The autoclaving of step (6) is concretely comprised the following steps：Treat steam pressure for 1.05kg/cm2-1.15kg/cm2When keep permanent
Pressure, when the temperature of vapor rises to 120 DEG C -122 DEG C, keeps sterilizing 18-22 minute under constant temperature, stops pressurization, treat pressure
Vapor is discharged after being reduced to 0.
Usage：The seed liquor of one or more lactic acid bacteria is added to the culture that the present invention effectively extends lactic acid bacteria survival period
In base, cultivate at a temperature of 35 DEG C -40 DEG C.
The pharmaceutical characteristic of each active component of the present invention is as follows：
Peptone：Peptone be will meat, the powder for drying after casein or gelatin acid or protease hydrolysiss, rich in having
Machine nitrogen compound, vitamin and saccharide, can be that microorganism provides C source, N source, life as the primary raw material of microbiological culture media
The nutrient substance such as the long factor.
Yeast extract：Yeast extract be with high protein bread yeast or beer yeast as raw material, through self-dissolving, enzymolysis, concentration,
A kind of rich in proteins, aminoacid, polypeptide, nucleic acid, the nutritional labeling such as electrolytes and minerals that technique makes such as dry
Biological culture base product.Yeast extract nutritional labeling eurythmy, can provide the nutrition of general equilibrium for fermentable culture.
Mannooligo saccharide：Mannooligo saccharide is the class novel antigens active substance for extracting from Yeast Cultivation cell wall, not only
Good physicochemical property with low grade fever, stable, safety non-toxic, and the effect with protection intestinal and enhance immunity.
Defatted milk powder：Defatted milk powder is the powdery product that fresh milk is sloughed fatty re-dry, contained protein
The 34% of non-fat solid is not less than, containing abundant trophic factors.The fat content of defatted milk powder is less, easy to maintain, is difficult
Generation Oxidation.
Defatted soybean meal：Defatted soybean meal refers to Semen sojae atricolor remaining side-product after immersion oil, the protein containing 46%, potassium, calcium
Deng inorganic salt and carbohydrate, diversified Speciality Foods are can be made into using it.Defatted soybean meal as a kind of high protein,
It is the primary raw material for making domestic animal and poultry feed, can be also used for making cake food, health food and cosmetics and anti-
Rhzomorph raw material.
Magnesium sulfate heptahydrate：Magnesium sulfate heptahydrate is the granular or rhomboidan in corner, and water white transparency is soluble in water, is slightly soluble in second
Alcohol and glycerol.For culture lactic acid bacteria, required trace element is provided in the present invention together with seven water manganese sulfates.
Seven water manganese sulfates：Seven water manganese sulfates are the peach powder for being concentrated using manganese oxide and manganese carbonate leaching extract
End.For culture lactic acid bacteria, required trace element is provided in the present invention together with Magnesium sulfate heptahydrate.
Dried Corn Steep Liquor Powder：Dried Corn Steep Liquor Powder is the powder being spray-dried as raw material through low temperature transient heating with fresh Semen Maydis pulp
End, its protein, micronutrient levelss height.Semen Maydis pulp is the very commonly used organic nitrogen source of growth of microorganism, and it can also promote
The biosynthesiss of the antibiotic such as penicillin.Make the nutrition such as water-soluble plant albumen and water soluble vitamins in biological fermentation process
Component extender.
Compound additive：Compound additive in the present invention be sodium carboxymethyl cellulose, sodium alginate, bone meal, Royal Jelly acid,
Guar gum and xanthan gum are 2 according to mass ratio：2：3：1：1：2 ratio is mixed.Sodium carboxymethyl cellulose is one in itself
Thickening agent is planted, the uniformity of product can be effectively improved, sodium carboxymethyl cellulose can form co-agglomeration thing with gelatin and pectin,
Complex can be formed with collagen, the albumen of some positively chargeds can be precipitated.Bone meal is that mammalian tissues and bone are passed through refining
Product after oil, dry and crushing, main component is tricalcium phosphate, osseocolla and fat.It is honeybee inside sodium alginate gel bead
Nest shape structure, after lactic acid bacteria is partially embedded through which, its heat stability and durable storage characteristics are all significantly improved.Royal Jelly acid (10-HDA)
Being that a kind of organic acid is isolated from Lac regis apis, be a kind of special unsaturated organic acid, is a kind of white crystal, fusing point 64
DEG C, Nature comparison is stable, the function of having good sterilization, bacteriostasis and anticancer, anti-radiation, in the environment of low temperature or high temperature
Can deposit for a long time and its structure is also not in the phenomenon that destroys or disappear.In the present invention by sodium carboxymethyl cellulose with
Sodium alginate, bone meal, Royal Jelly acid, guar gum and xanthan gum are used in mixed way, unit viscosity 2-3 higher than sodium carboxymethyl cellulose
Times.
Sodium dihydrogen phosphate：Sodium dihydrogen phosphate used by the present invention is acid-base buffer agent, and sodium dihydrogen phosphate is colourless iris
Crystallization, is mainly used in process hides, processes boiler water, as quality improver and baking powder processed, delay in food industry, fermentation industry
Electuary and fermentation powder raw material, also serve as feed additive, detergent and dye auxiliary agent etc..
Sodium citrate：Sodium citrate is a kind of organic compound of white crystalline, uses the style in food, beverage industry
Taste agent, stabilizer；It is used as anticoagulant, apophlegmatisant and diuretic in medical industry；Wash as nontoxic in detergent industry
Wash auxiliary agent of agent etc..It is all culture lactic acid bacteria in the present invention with citric acid and sodium acetate etc. and somatomedin is provided, which is effective
Composition can also maintain balanced osmotic pressure, suppress miscellaneous bacteria.
Citric acid：Citric acid is a kind of important organic acid, at room temperature for semi-transparent clear crystal or white particle or
White crystalline powder, odorless, soluble in water.It is all culture lactic acid bacteria in the present invention to carry with sodium citrate and sodium acetate etc.
For somatomedin, its effective ingredient can also maintain balanced osmotic pressure, suppress miscellaneous bacteria.
Sodium acetate：Sodium acetate is the crystalline solid of colorless and odorless, is mainly used in printing and dyeing industry, medicine, photograph, plating, chemistry
Reagent and organic synthesiss etc..It is all culture lactic acid bacteria in the present invention with sodium citrate and citric acid etc. and somatomedin is provided,
Its effective ingredient can also maintain balanced osmotic pressure, suppress miscellaneous bacteria.
Tween 80：It is faint yellow to orange-yellow thick liquid that Tween 80 is surfactant, Tween 80 in the present invention,
Mildly bitter flavor is slightly puckery, and the lipophilic composition in Tween 80 includes unsaturated fatty acid etc..
Saccharomyces cerevisiae：Saccharomyces cerevisiae is the most frequently used biological species in fermentation, is conventionally used to make bread and steamed bread etc.
Food and wine brewing.For culture lactic acid bacteria, carbon source, nitrogen source are provided in the present invention.
The Advantageous Effects of the present invention：Effective culture medium for extending lactic acid bacteria survival period, is soaked with peptone, yeast
Powder, mannooligo saccharide, defatted milk powder, defatted soybean meal, Magnesium sulfate heptahydrate, compound additive, citric acid, sodium acetate, saccharomyces cerevisiae etc.
Feed purification is formed.Wherein, for culture lactic acid bacteria, carbon source and nitrogen source are provided together with saccharomyces cerevisiae and mannooligo saccharide etc.；Seven water sulphuric acid
Magnesium and seven water manganese sulfates provide multiple required trace element for culture lactic acid bacteria together；Sodium citrate, citric acid and sodium acetate
Somatomedin is provided for cultivating lactic acid bacteria, its composition can also maintain balanced osmotic pressure, suppress miscellaneous bacteria.All raw materials are mutually cooperateed with, energy
Long-time can keep higher viable bacteria amount at normal temperatures to make liquid lactic acid bacterium, and easy to operate, and raw material is easy to get, with low cost, energy
The property that makes lactic acid bacteria is more stable, biological activity is higher, it is more permanent to store.
Effective culture medium for extending lactic acid bacteria survival period, the saccharomyces cerevisiae for using can be secreted nutrient substance and strengthen lactic acid
The vigor of bacterium, delays the decline time of lactic acid bacteria, while carbon source and nitrogen source is provided for culture lactic acid bacteria together with mannooligo saccharide etc.,
And mannooligo saccharide has β -1,3 glucosans and β -1 simultaneously, 6 glucosan functions, effectively increase lactic acid bacteria intake carbon source
Channel, so as to increased viable bacteria amount.Effective culture medium for extending lactic acid bacteria survival period, also coordinates while using defatted milk powder
Defatted soybean meal is employed, the protein needed for not only meeting during lactobacter growth, and can be the growth of lactic acid bacteria
Enough abundant trophic factors are provided, so as to, while lactic acid bacteria nutritional labeling is ensured, significantly reduce cost, while
Improve culture effect.Effective culture medium for extending lactic acid bacteria survival period, employs the rational compound additive of collocation, described
Compound additive is a certain proportion of sodium carboxymethyl cellulose, sodium alginate, bone meal, Royal Jelly acid, guar gum and xanthan gum
Mixture.Sodium carboxymethyl cellulose is a kind of thickening agent, can effectively improve the uniformity of product, sodium carboxymethyl cellulose and gelatin
And pectin can form co-agglomeration thing, it is also possible to form complex with collagen, the albumen of some positively chargeds, sodium alginate can be precipitated
It is alveolate texture inside gelled pill, after lactic acid bacteria is partially embedded through which, its heat stability and durable storage characteristics are significantly improved；
Royal Jelly acid has the function of good sterilization, bacteriostasis and anticancer, anti-radiation, can long-time in the environment of low temperature or high temperature
Storage and its structure also be not in destroy or disappear phenomenon, used in the present invention, Royal Jelly acid can be miscellaneous to escherichia coli etc.
Bacterium produces inhibitory action, and lactic acid bacteria acid resistance itself is preferable, is unaffected, and the use of Royal Jelly acid not only ensure that food
Safety, and competition of the harmful bacteria to nutrient substance is prevented, so as to promote the growth of viable count of lactobacillus；In the present invention
Sodium carboxymethyl cellulose is used in mixed way with sodium alginate, bone meal, Royal Jelly acid, guar gum and xanthan gum, unit viscosity than
Sodium carboxymethyl cellulose is high 2-3 times, and can be prevented effectively from the phenomenon for nutrient substance and lactic acid bacteria occur in suspended state, from
And so that nutrient substance is fully utilized, and then extend the storage life of Lactobacillus.
Specific embodiment
With reference to embodiment, the invention will be further described, but the invention is not limited in this.
Embodiment 1：Effective culture medium for extending lactic acid bacteria survival period, the raw material for taking following portions by weight is prepared from
(per part takes 30g)：0.08 part of peptone, 0.08 part of yeast extract, 1.5 parts of mannooligo saccharide, 0.8 part of defatted milk powder, defatted soybean meal
0.5 part, 0.05 part of Magnesium sulfate heptahydrate, 0.025 part of seven water manganese sulfate, 1.5 parts of Dried Corn Steep Liquor Powder, 0.008 part of compound additive, phosphorus
0.025 part of acid dihydride sodium, 0.25 part of sodium citrate, 0.2 part of citric acid, 0.08 part of sodium acetate, Tween 80 are 0.08 part, wine brewing ferment
Female 0.04 part；The compound additive is sodium carboxymethyl cellulose, sodium alginate, bone meal, Royal Jelly acid, guar gum and xanthan gum
Mixture, and mass ratio be sodium carboxymethyl cellulose：Sodium alginate：Bone meal：Royal Jelly acid：Guar gum：Xanthan gum=2：2：
3：1：1：2；The colony density of the saccharomyces cerevisiae is 2 × 105cfu/g.
The preparation method of above-mentioned effective culture medium for extending lactic acid bacteria survival period, using following steps：
(1) Dried Corn Steep Liquor Powder is placed in agitator tank, 8 DEG C of 4 times of Dried Corn Steep Liquor Powder quality of purified water is added, is stirred into
Pasty state, after placing 20 minutes, adds 15 DEG C of 2 times of Dried Corn Steep Liquor Powder quality of purified water, stirs；
(2) successively by peptone, yeast extract, mannooligo saccharide, defatted milk powder, defatted soybean meal, Magnesium sulfate heptahydrate, seven water sulfur
Sour manganese, step (1) products therefrom, sodium dihydrogen phosphate, sodium citrate, citric acid, sodium acetate, Tween 80 and colony density be 2 ×
105The saccharomyces cerevisiae of cfu/g (is that saccharomyces cerevisiae is placed in culture gained, wherein saccharomyces cerevisiae in saccharomyces cerevisiae fluid medium
The composition of fluid medium is 0.5 part of peptone, 1 part of glucose, 0.3 part of yeast extract, 0.3 part of beerwort) it is put into beaker
In, 17 DEG C of 0.6 times of mixture quality of purified water is added, is stirred, obtain mixed liquor；
(3) by the sodium carboxymethyl cellulose in compound additive, sodium alginate, bone meal, Royal Jelly acid, guar gum and xanthan
Glue is 2 in mass ratio：2：3：1：1：2 ratio mix homogeneously, is added in the pure water of 80 times of compound additive quality, fully
Mix dissolving；
(4) products therefrom in step (3) is added in step (2) in the beaker for filling in mixed liquor, mix homogeneously, will
The beaker for filling mixed material after mix homogeneously is placed in and is covered with the electric furnace of asbestos gauge, and small fire is heated, the glass in heating
Rod is stirred 25 minutes clockwise, stops heating；
(5) HCl solution that mass concentration is 5% is added toward in step (4) products therefrom, it is 5.4 to adjust pH value；
(6) step (5) products therefrom autoclaving (is treated steam pressure for 1.08kg/cm2When keep constant voltage, treat water steam
When the temperature of gas rises to 120 DEG C, keep sterilizing 18 minutes under constant temperature, stop pressurization, vapor is discharged after pressure is reduced to 0)
18 minutes, obtain final product effective culture medium for extending lactic acid bacteria survival period.
Usage：The seed liquor of certain or various lactobacillus is added to the culture of effective prolongation lactic acid bacteria survival period of gained
In base, cultivate at a temperature of 37 DEG C.
Embodiment 2：Effective culture medium for extending lactic acid bacteria survival period, its active component is made up of the raw material of following weight
(per part takes 15g)：0.12 part of peptone, 0.12 part of yeast extract, 2.5 parts of mannooligo saccharide, 1.2 parts of defatted milk powder, defatted soybean meal
0.7 part, 0.07 part of Magnesium sulfate heptahydrate, 0.035 part of seven water manganese sulfate, 2.5 parts of Dried Corn Steep Liquor Powder, 0.012 part of compound additive, phosphorus
0.035 part of acid dihydride sodium, 0.35 part of sodium citrate, 0.3 part of citric acid, 0.12 part of sodium acetate, Tween 80 are 0.12 part, wine brewing ferment
Female 0.06 part；The compound additive is sodium carboxymethyl cellulose, sodium alginate, bone meal, Royal Jelly acid, guar gum and xanthan gum
Mixture, and mass ratio be sodium carboxymethyl cellulose：Sodium alginate：Bone meal：Royal Jelly acid：Guar gum：Xanthan gum=2：2：
3：1：1：2；The colony density of the saccharomyces cerevisiae is 2 × 105cfu/g.
(1) Dried Corn Steep Liquor Powder is placed in agitator tank, adds 12 DEG C of 6 times of Dried Corn Steep Liquor Powder quality of purified water, stirring
Become pasty state, 20 DEG C of 4 times of Dried Corn Steep Liquor Powder quality of purified water after placing 30 minutes, is added, is stirred；
(2) successively by peptone, yeast extract, mannooligo saccharide, defatted milk powder, defatted soybean meal, Magnesium sulfate heptahydrate, seven water sulfur
Sour manganese, step (1) products therefrom, sodium dihydrogen phosphate, sodium citrate, citric acid, sodium acetate, Tween 80 and colony density be 2 ×
105The saccharomyces cerevisiae of cfu/g (is that saccharomyces cerevisiae is placed in culture gained, wherein saccharomyces cerevisiae in saccharomyces cerevisiae fluid medium
The composition of fluid medium is 0.5 part of peptone, 1 part of glucose, 0.3 part of yeast extract, 0.3 part of beerwort) it is put into beaker
In, 17 DEG C of 0.7 times of mixture quality of purified water is added, is stirred, obtain mixed liquor；
(3) by the sodium carboxymethyl cellulose in compound additive, sodium alginate, bone meal, Royal Jelly acid, guar gum and xanthan
Glue is 2 in mass ratio：2：3：1：1：2 ratio mix homogeneously, is added in the pure water of 100 times of compound additive quality, fills
Divide and mix dissolving；
(4) products therefrom in step (3) is added in step (2) in the beaker for filling in mixed liquor, mix homogeneously, will
The beaker for filling mixed material after mix homogeneously is placed in and is covered with the electric furnace of asbestos gauge, and small fire is heated, the glass in heating
Rod is stirred 35 minutes clockwise, stops heating；
(5) HCl solution that mass concentration is 7% is added toward in step (4) products therefrom, it is 5.8 to adjust pH value；
(6) step (5) products therefrom autoclaving (is treated steam pressure for 1.13kg/cm2When keep constant voltage, treat water steam
When the temperature of gas rises to 122 DEG C, keep sterilizing 22 minutes under constant temperature, stop pressurization, vapor is discharged after pressure is reduced to 0)
22 minutes, obtain final product effective culture medium for extending lactic acid bacteria survival period.
Usage is with embodiment 1.
Embodiment 3：Effective culture medium for extending lactic acid bacteria survival period, its active component is made up of the raw material of following weight
(per part takes 25kg)：0.09 part of peptone, 0.09 part of yeast extract, 1.8 parts of mannooligo saccharide, 0.9 part of defatted milk powder, defatted soybean meal
0.55 part, 0.055 part of Magnesium sulfate heptahydrate, 0.028 part of seven water manganese sulfate, 1.8 parts of Dried Corn Steep Liquor Powder, 0.009 part of compound additive,
0.028 part of sodium dihydrogen phosphate, 0.28 part of sodium citrate, 0.23 part of citric acid, 0.09 part of sodium acetate, Tween 80 are 0.09 part, make
0.045 part of brewer yeast；The compound additive be sodium carboxymethyl cellulose, sodium alginate, bone meal, Royal Jelly acid, guar gum and
The mixture of xanthan gum, and mass ratio is sodium carboxymethyl cellulose：Sodium alginate：Bone meal：Royal Jelly acid：Guar gum：Xanthan gum
=2：2：3：1：1：2；The colony density of the saccharomyces cerevisiae is 2 × 105cfu/g.
(1) Dried Corn Steep Liquor Powder is placed in agitator tank, adds 10 DEG C of 5 times of Dried Corn Steep Liquor Powder quality of purified water, stirring
Become pasty state, 18 DEG C of 3 times of Dried Corn Steep Liquor Powder quality of purified water after placing 25 minutes, is added, is stirred；
(2) successively by peptone, yeast extract, mannooligo saccharide, defatted milk powder, defatted soybean meal, Magnesium sulfate heptahydrate, seven water sulfur
Sour manganese, step (1) products therefrom, sodium dihydrogen phosphate, sodium citrate, citric acid, sodium acetate, Tween 80 and colony density be 2 ×
105The saccharomyces cerevisiae of cfu/g (is that saccharomyces cerevisiae is placed in culture gained, wherein saccharomyces cerevisiae in saccharomyces cerevisiae fluid medium
The composition of fluid medium is 0.5 part of peptone, 1 part of glucose, 0.3 part of yeast extract, 0.3 part of beerwort) it is put into beaker
In, 17 DEG C of 0.7 times of mixture quality of purified water is added, is stirred, obtain mixed liquor；
(3) by the sodium carboxymethyl cellulose in compound additive, sodium alginate, bone meal, Royal Jelly acid, guar gum and xanthan
Glue is 2 in mass ratio：2：3：1：1：2 ratio mix homogeneously, is added in the pure water of 90 times of compound additive quality, fully
Mix dissolving；
(4) products therefrom in step (3) is added in step (2) in the beaker for filling in mixed liquor, mix homogeneously, will
The beaker for filling mixed material after mix homogeneously is placed in and is covered with the electric furnace of asbestos gauge, and small fire is heated, the glass in heating
Rod is stirred 30 minutes clockwise, stops heating；
(5) HCl solution that mass concentration is 6% is added toward in step (4) products therefrom, it is 5.6 to adjust pH value；
(6) step (5) products therefrom autoclaving (is treated steam pressure for 1.1kg/cm2When keep constant voltage, treat vapor
Temperature when rising to 121 DEG C, keep sterilizing 20 minutes under constant temperature, stop pressurization, discharge vapor after pressure is reduced to 0) 20
Minute, obtain final product effective culture medium for extending lactic acid bacteria survival period.
Usage is with embodiment 1.
Embodiment 4：Effective culture medium for extending lactic acid bacteria survival period, its active component is made up of the raw material of following weight
(per part takes 25kg)：0.11 part of peptone, 0.11 part of yeast extract, 2.2 parts of mannooligo saccharide, 1.1 parts of defatted milk powder, defatted soybean meal
0.65 part, 0.065 part of Magnesium sulfate heptahydrate, 0.032 part of seven water manganese sulfate, 2.2 parts of Dried Corn Steep Liquor Powder, 0.011 part of compound additive,
0.032 part of sodium dihydrogen phosphate, 0.32 part of sodium citrate, 0.27 part of citric acid, 0.11 part of sodium acetate, Tween 80 are 0.11 part, make
0.055 part of brewer yeast；The compound additive be sodium carboxymethyl cellulose, sodium alginate, bone meal, Royal Jelly acid, guar gum and
The mixture of xanthan gum, and mass ratio is sodium carboxymethyl cellulose：Sodium alginate：Bone meal：Royal Jelly acid：Guar gum：Xanthan gum
=2：2：3：1：1：2；The colony density of the saccharomyces cerevisiae is 2 × 105cfu/g.
Preparation method is with usage with embodiment 3.
Embodiment 5：Effective culture medium for extending lactic acid bacteria survival period, its active component is made up of the raw material of following weight
(per part takes 25kg)：0.1 part of peptone, 0.1 part of yeast extract, 2 parts of mannooligo saccharide, 1 part of defatted milk powder, 0.6 part of defatted soybean meal,
0.06 part of Magnesium sulfate heptahydrate, 0.03 part of seven water manganese sulfate, 2 parts of Dried Corn Steep Liquor Powder, 0.01 part of compound additive, sodium dihydrogen phosphate
0.03 part, 0.3 part of sodium citrate, 0.25 part of citric acid, 0.1 part of sodium acetate, Tween 80 be 0.1 part, 0.05 part of saccharomyces cerevisiae, 0.25 part of citric acid, 0.1 part of sodium acetate, Tween 80 be 0.1 part, 0.05 part of saccharomyces cerevisiae, 0.25 part of citric acid, 0.1 part of sodium acetate, Tween 80 be 0.1 part, 0.05 part of saccharomyces cerevisiae, 0.25 part of citric acid, 0.1 part of sodium acetate, Tween 80 be 0.1 part, 0.05 part of saccharomyces cerevisiae, 0.25 part of citric acid, 0.1 part of sodium acetate, Tween 80 be 0.1 part, 0.05 part of saccharomyces cerevisiae, 0.25 part of citric acid, 0.1 part of sodium acetate, Tween 80 be 0.1 part, 0.05 part of saccharomyces cerevisiae, 0.25 part of citric acid, 0.1 part of sodium acetate, Tween 80 be 0.1 part, 0.05 part of saccharomyces cerevisiae, 0.25 part of citric acid, 0.1 part of sodium acetate, Tween 80 be 0.1 part, 0.05 part of saccharomyces cerevisiae, 0.25 part of citric acid, 0.1 part of sodium acetate, Tween 80 be 0.1 part, 0.05 part of saccharomyces cerevisiae, 0.25 part of citric acid, 0.1 part of sodium acetate, Tween 80 be 0.1 part, 0.05 part of saccharomyces cerevisiae, 0.25 part of citric acid, 0.1 part of sodium acetate, Tween 80 be 0.1 part, 0.05 part of saccharomyces cerevisiae, 0.25 part of citric acid, 0.1 part of sodium acetate, Tween 80 be 0.1 part, 0.05 part of saccharomyces cerevisiae, 0.25 part of citric acid, 0.1 part of sodium acetate, Tween 80 be 0.1 part, 0.05 part of saccharomyces cerevisiae, 0.25 part of citric acid, 0.1 part of sodium acetate, Tween 80 be 0.1 part, 0.05 part of saccharomyces cerevisiae, 0.25 part of citric acid, 0.1 part of sodium acetate, Tween 80 be 0.1 part, 0.05 part of saccharomyces cerevisiae, 0.25 part of citric acid, 0.1 part of sodium acetate, Tween 80 be 0.1 part, 0.05 part of saccharomyces cerevisiae, 0.25 part of citric acid, 0.1 part of sodium acetate, Tween 80 be 0.1 part, 0.05 part of saccharomyces cerevisiae, 0.25 part of citric acid, 0.1 part of sodium acetate, Tween 80 be 0.1 part, 0.05 part of saccharomyces cerevisiae, 0.25 part of citric acid, 0.1 part of sodium acetate, Tween 80 be 0.1 part, 0.05 part of saccharomyces cerevisiae, 0.25 part of citric acid, 0.1 part of sodium acetate, Tween 80 be 0.1 part, 0.05 part of saccharomyces cerevisiae, 0.25 part of citric acid, 0.1 part of sodium acetate, Tween 80 be 0.1 part, 0.05 part of saccharomyces cerevisiae, 0.25 part of citric acid, 0.1 part of sodium acetate, Tween 80 be 0.1 part, 0.05 part of saccharomyces cerevisiae, 0.25 part of citric acid, 0.1 part of sodium acetate, Tween 80 be 0.1 part, 0.05 part of saccharomyces cerevisiae, 0.25 part of citric acid, 0.1 part of sodium acetate, Tween 80 be 0.1 part, 0.05 part of saccharomyces cerevisiae, 0.25 part of citric acid, 0.1 part of sodium acetate, Tween 80 be 0.1；Institute
The mixture that compound additive is sodium carboxymethyl cellulose, sodium alginate, bone meal, Royal Jelly acid, guar gum and xanthan gum is stated, and
Mass ratio is sodium carboxymethyl cellulose：Sodium alginate：Bone meal：Royal Jelly acid：Guar gum：Xanthan gum=2：2：3：1：1：2；Described
The colony density of saccharomyces cerevisiae is 2 × 105cfu/g.
Preparation method is with usage with embodiment 3.
Test example：
For a kind of effective culture medium for extending lactic acid bacteria survival period of gained of the present invention, inventor has done Contrast on effect reality
Test.
Lactobacillus bulgaricus are placed in lactobacillus solution body culture medium, are cultivated, obtain lactobacillus solution.Its
The concrete composition of middle lactobacillus solution body culture medium is：1 part of peptone, 1 part of Carnis Bovis seu Bubali cream, 0.5 part of yeast extract, phosphoric acid hydrogen
0.2 part of dipotassium, 0.2 part of Triammonium citrate, 0.5 part of sodium acetate, 2 parts of glucose, 0.1 part of Tween 80, Magnesium sulfate heptahydrate 0.06
Part, 0.025 part of four water manganese sulfate.
Take gained of the present invention respectively and effectively extend the culture medium (embodiment 1-5) of lactic acid bacteria survival period as experimental group, right
The culture medium of 3 kinds of forms is adopted according to group, respectively：Matched group 1 is using classical formula MRS lactic acid bacteria culture medium, per liter of MRS breast
Acetic bacterial culture medium prescription (g/L)：Peptone 10.0g, Carnis Bovis seu Bubali cream 10.0g, yeast extract 5.0g, diammonium hydrogen citrate [(NH4)2HC6H5O7] 2.0g, glucose (C6H12O6·H2O) 20.0g, Tween 80 are 1.0mL, sodium acetate (CH3COONa·3H2O)5.0g、
Dipotassium hydrogen phosphate (K2HPO4·3H2O) 2.0g, magnesium sulfate (MgSO4·7H2O) 0.58g, manganese sulfate (MnSO4·H2O)0.25g、
Agar 18.0g, distilled water 1000mL；PH is 6.2-6.6.2 used medium of matched group is available from Qingdao sea wins biotechnology has
Limit company, its formula is (g/L)：Peptone 10.0；Beef extract powder 5.0；Yeast extract 4.0；Glucose 20.0；Phosphoric acid hydrogen two
Potassium 2.0；Triammonium citrate 2.0；Sodium acetate 5.0；Magnesium sulfate 0.2；Manganese sulfate 0.05；Agar 15.0；Tween 80 is 1.0；pH6.2
±0.2；25℃.3 used medium of matched group is to deduct sodium carboxymethyl cellulose and royal jelly in the composition of culture medium of the present invention
Prepared by after acid.
Impact of 1.1 culture medium of the present invention to lactic acid bacteria bacterium amount
Respectively the mixing lactic acid bacteria seed liquor of 0.25 times of culture medium quality is added to the corresponding of experimental group and matched group
In culture medium, cultivate 36 hours at a temperature of 37 DEG C, aseptic subpackaged, while determining lactic acid bacteria clump count respectively, then distinguish
Store 9 months under 25 DEG C, 4 DEG C, 37 DEG C of environment, in the time point determining lactic acid bacteria bacterium amount that each sets, while determining 25 DEG C
Varied bacteria growing situation (is represented as miscellaneous bacteria using escherichia coli and is counted)；Fermentation liquid was chosen at 25 DEG C the 4th day, the bottom of respectively at
Portion, 1/4,2/4 ,/3/4,5, top height sample, determine the position fermented product density, and average, concrete condition is shown in
Shown in table 1- table 5：
Lactic acid bacteria bacterium amount (the unit of 1 different time points of table：Cfu/g, 25 DEG C)
By table 1, at 25 DEG C, the effect of experimental group and matched group is statistically analyzed, two groups of effects have significant difference
(p ＜ 0.05), the mortality rate of the lactic acid bacteria in experimental group is declined to a great extent compared with matched group, wherein, using 5 institute of embodiment
When the 270th day after the culture medium culturing mixing lactic acid bacteria seed liquor of the effective prolongation lactic acid bacteria survival period for obtaining, viable count of lactobacillus
It is still 5.8 × 107Cfu/g, meets GB 19302-2010 national food safety standard《Fermentation milk》Relevant criterion；And adopt right
During according to organizing the 270th day after used culture medium culturing mixing lactic acid bacteria seed liquor, viable count of lactobacillus is reduced to 0,1.2 respectively
×102Cfu/g and 9.8 × 102cfu/g.Obviously, a kind of effective culture medium for extending lactic acid bacteria survival period of gained of the present invention is than existing
The prolongation lactic acid bacteria survival period of the MRS lactic acid bacteria culture medium for improveing in some MRS lactic acid bacteria culture medium and prior art
Effect is significant.Meanwhile, the concrete condition of contrast experiment's group and matched group 3, add in the culture medium used by experimental group (embodiment 1-5)
Sodium carboxymethyl cellulose and Royal Jelly acid are added, and these compositions in the culture medium used by matched group 3, have been not added with, by the tool in table 1
Body numerical value can be seen that the time point for setting using the viable lactic acid bacteria of gained culture medium (embodiment 1-5) of the present invention at each
There is higher content, the mortality rate of lactic acid bacteria is declined to a great extent compared with matched group 3.
Lactic acid bacteria bacterium amount (the unit of 2 different time points of table：Cfu/g, 4 DEG C)
By table 2, at 4 DEG C, the effect of experimental group and matched group is statistically analyzed, two groups of effects have significant difference (p
＜ 0.05), under the mortality rate of the lactic acid bacteria in experimental group is had significantly compared with matched group.Wherein, using 5 gained of embodiment
When effectively the 270th day after the culture medium culturing mixing lactic acid bacteria seed liquor of prolongation lactic acid bacteria survival period, viable count of lactobacillus is still
6.3×107Cfu/g, meets GB 19302-2010 national food safety standard《Fermentation milk》Relevant criterion；And adopt matched group
When the 270th day after the culture medium culturing mixing lactic acid bacteria seed liquor for being used, viable count of lactobacillus is reduced to 0,1.5 respectively ×
102Cfu/g and 1.3 × 103cfu/g.Obviously, a kind of effective culture medium for extending lactic acid bacteria survival period of gained of the present invention is than existing
MRS lactic acid bacteria culture medium and prior art in improve MRS lactic acid bacteria culture medium prolongation lactic acid bacteria survival period effect
Fruit is notable.Meanwhile, the concrete condition of contrast experiment's group and matched group 3, add in the culture medium used by experimental group (embodiment 1-5)
Sodium carboxymethyl cellulose and Royal Jelly acid, and these compositions in the culture medium used by matched group 3, are not added with, by concrete in table 2
Numerical value can be seen that the time point for setting using the viable lactic acid bacteria of gained culture medium (embodiment 1-5) of the present invention at each
There is higher content, the mortality rate of lactic acid bacteria is declined to a great extent compared with matched group 3.
Lactic acid bacteria bacterium amount (the unit of 3 different time points of table：Cfu/g, 37 DEG C)
By table 3, at 37 DEG C, the effect of experimental group and matched group is statistically analyzed, two groups of effects have significant difference
(p ＜ 0.05), under the mortality rate of the lactic acid bacteria in experimental group is had significantly compared with matched group.Wherein, using 5 gained of embodiment
Effective prolongation lactic acid bacteria survival period culture medium culturing mixing lactic acid bacteria seed liquor after the 21st day when, viable count of lactobacillus is still
5.7×106Cfu/g, and when adopting the 17th day after the culture medium culturing mixing lactic acid bacteria seed liquor used by matched group, lactic acid bacteria
Viable count is just all reduced to 0.Obviously, a kind of effective culture medium for extending lactic acid bacteria survival period of gained of the present invention is than existing MRS
The MRS lactic acid bacteria culture medium for improveing in lactic acid bacteria culture medium, prior art and the prolongation breast of the culture medium of other components
Sour bacterium survival period effect is significant.Can be seen that using gained culture medium (embodiment 1-5) of the present invention by the concrete numerical value in table 3
Viable lactic acid bacteria have higher content in each time point for setting.
To sum up, can be seen that either at a temperature of 25 DEG C, 4 DEG C or 37 DEG C by table 1-3, gained one kind of the present invention
Effectively extend MRS breast of the culture medium of lactic acid bacteria survival period all than improveing in existing MRS lactic acid bacteria culture medium, prior art
The prolongation lactic acid bacteria survival period effect is significant of acetic bacterial culture medium and other culture medium.And, as shown in Table 3, institute of the present invention
Obtain culture medium and can will bring up to for 3 week the shelf-life under conditions of extreme temperature (37 DEG C), greatly facilitate the use of user.
Coliform group count (the unit of 4 different time points of table：Cfu/g, 25 DEG C)
Note：- represent severe overweight, no longer count.
The effect of experimental group and matched group is statistically analyzed, two groups of effects have significant difference (p ＜ 0.05), by table 4
As can be seen that the coliform group count in experimental group is few more than the coliform group count of matched group.Wherein, at the 5th day, institute of the present invention
Culture medium all there is not coliform, also only having in 3 gained culture medium of embodiment when the 7th day has a small amount of coliform to occur, and right
Just there is at the 3rd day coliform to occur according to group, and just have in 5 natural law substantial amounts of coliform to occur.Can be inferred that Royal Jelly acid
Addition cause gained culture medium of the present invention control escherichia coli cultivation effect more significantly (sodium carboxymethyl cellulose be a kind of increasing
Thick dose, Royal Jelly acid has good sterilization, bacteriostasis, and used in the present invention, Royal Jelly acid can produce suppression to miscellaneous bacterias such as escherichia coli
Make and use, and lactic acid bacteria acid resistance itself is preferable, is unaffected), competition of the harmful bacteria to nutrient substance is effectively prevent,
So as to promote the growth of viable count of lactobacillus.
5 fermentation liquid differing heights product density of table (25 DEG C, the 4th day)
The effect of experimental group and matched group is statistically analyzed, two groups of effects have significant difference (p ＜ 0.05), the 4th
It when choose fermentation liquid, respectively at bottom, 1/4,2/4 ,/3/4,5, top height sample, determine the position fermented product close
Degree.From standard deviation size, it is apparent that the density of each height of experimental group is essentially identical, its difference comes from measurement error,
And meet normal distribution.And matched group is with the rising of height, density is gradually reduced, and trend is fairly obvious, standard deviation is 1.9 ×
10-3-2.2×10-3, hence it is evident that more than the 0.3 × 10 of experimental group-3-0.4×10-3.It can be seen that, due to the addition of sodium carboxymethyl cellulose
(Royal Jelly acid has good sterilization, bacteriostasis, and used in the present invention, Royal Jelly acid is permissible to considerably increase the homogeneity of tunning
Inhibitory action is produced to miscellaneous bacterias such as escherichia coli；And sodium carboxymethyl cellulose is a kind of thickening agent, the equal of product can be effectively improved
Once, sodium carboxymethyl cellulose can form co-agglomeration thing with gelatin and pectin, it is also possible to form complex with collagen, can precipitate
The albumen of some positively chargeds, is alveolate texture inside sodium alginate gel bead, and after lactic acid bacteria is partially embedded through which, its heat is steady
Qualitative and durable storage characteristics are significantly improved), it is therefore prevented that bottom cell density is excessive to cause nutrient competition, and so that nutrient substance is all filled
Divide and utilize.
To sum up, can draw from table 1- table 5, gained of the present invention effectively extends the culture medium of lactic acid bacteria survival period and effectively can carry
The survival rate of lactic acid bacteria in high liquid lactic acid bacterium, the property that can make lactic acid bacteria is more stable, biological activity is higher, storage is more permanent,
Easily stored, in use because giving full play to its treatment and health-care effect containing enough Lactobacillus.The present invention
Gained effectively extends the culture medium of lactic acid bacteria survival period to the popularization in health food and animal additive agent field and using having
Significance.
Finally it should be noted that embodiment is the specific embodiment of present invention optimum, this is not limited to
Invention, although being described in detail to the present invention with reference to the foregoing embodiments, for a person skilled in the art, its according to
So the technical scheme described in foregoing embodiments can be modified, or equivalent is carried out to which part technical characteristic and replace
Change.All any modification, equivalent substitution and improvement that within the spirit and principles in the present invention, is made etc., should be included in the present invention
Protection domain within.
Claims (8)
1. a kind of effective culture medium for extending lactic acid bacteria survival period, is characterized in that：Its active component is by the original of following parts by weight
Material is made：Peptone 0.08-0.12 part, yeast extract 0.08-0.12 part, mannooligo saccharide 1.5-2.5 part, defatted milk powder 0.16-
0.24 part, defatted soybean meal 0.5-0.7 part, Magnesium sulfate heptahydrate 0.05-0.07 part, seven water manganese sulfate 0.025-0.035 parts, Semen Maydis pulp
Dry powder 1.5-2.5 part, compound additive 0.008-0.012 part, sodium dihydrogen phosphate 0.025-0.035 part, sodium citrate 0.25-
0.35 part, citric acid 0.2-0.3 part, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12 part, Tween 80 be 0.08-0.12 part, saccharomyces cerevisiae 0.04-, sodium acetate 0.08-0.12
0.06 part.
2. a kind of effective culture medium for extending lactic acid bacteria survival period according to claim 1, is characterized in that：Its active component
It is made up of the raw material of following parts by weight：0.1 part of peptone, 0.1 part of yeast extract, 2 parts of mannooligo saccharide, 0.2 part of defatted milk powder,
0.6 part of defatted soybean meal, 0.06 part of Magnesium sulfate heptahydrate, 0.03 part of seven water manganese sulfate, 2 parts of Dried Corn Steep Liquor Powder, compound additive 0.01,
0.03 part of sodium dihydrogen phosphate, 0.3 part of sodium citrate, 0.25 part of citric acid, 0.1 part of sodium acetate, Tween 80 are 0.1 part, wine brewing ferment
Female 0.05 part.
3., according to the culture medium of the arbitrary described a kind of effective prolongation lactic acid bacteria survival period of claim 1-2, it is characterized in that：Described
Compound additive is the mixture of sodium carboxymethyl cellulose, sodium alginate, bone meal, Royal Jelly acid, guar gum and xanthan gum, and matter
Amount ratio is sodium carboxymethyl cellulose：Sodium alginate：Bone meal：Royal Jelly acid：Guar gum：Xanthan gum=2：2：3：1：1：2.
4., according to the culture medium of the arbitrary described a kind of effective prolongation lactic acid bacteria survival period of claim 1-2, it is characterized in that：Described
The colony density of saccharomyces cerevisiae is 2 × 105cfu/g.
5. according to the preparation method of the culture medium of the arbitrary described a kind of effective prolongation lactic acid bacteria survival period of claim 1-2, its
It is characterized in that：Comprise the steps：
(1) Dried Corn Steep Liquor Powder is placed in agitator tank, 8 DEG C -12 DEG C of 4-6 times of Dried Corn Steep Liquor Powder quality of purified water is added, is stirred
Pasty state is mixed, 15 DEG C -20 DEG C of 2-4 times of Dried Corn Steep Liquor Powder quality of purified water after placing 20-30 minute, is added, is stirred；
(2) successively by peptone, yeast extract, mannooligo saccharide, defatted milk powder, defatted soybean meal, Magnesium sulfate heptahydrate, seven water sulphuric acid
Manganese, step (1) products therefrom, sodium dihydrogen phosphate, sodium citrate, citric acid, sodium acetate, Tween 80 and saccharomyces cerevisiae are put into beaker
In, 15 DEG C -18 DEG C of 0.6-0.8 times of mixture quality of purified water is added, is stirred, obtain mixed liquor；
(3) sodium carboxymethyl cellulose in compound additive, sodium alginate, bone meal, Royal Jelly acid, guar gum and xanthan gum are pressed
Mass ratio is 2：2：3：1：1：2 ratio mix homogeneously, is added in the pure water of 80-100 times of compound additive quality, fully
Mix dissolving；
(4) products therefrom in step (3) is added in step (2) in the beaker for filling in mixed liquor, mix homogeneously, will mixing
The beaker for filling mixed material after uniform is placed in and is covered with the electric furnace of asbestos gauge, and small fire is heated, suitable with Glass rod in heating
Hour hands stir 25-35 minute, stop heating；
(5) toward in step (4) products therefrom, add mass concentration for the HCl solution of 5%-7%, regulation pH value is 5.4-5.8；
(6) by step (5) products therefrom autoclaving 18-22 minute, effective culture medium for extending lactic acid bacteria survival period is obtained final product.
6. a kind of preparation method of effective culture medium for extending lactic acid bacteria survival period according to claim 5, is characterized in that：
The compound additive is the mixture of sodium carboxymethyl cellulose, sodium alginate, bone meal, Royal Jelly acid, guar gum and xanthan gum,
And mass ratio is sodium carboxymethyl cellulose：Sodium alginate：Bone meal：Royal Jelly acid：Guar gum：Xanthan gum=2：2：3：1：1：2.
7. a kind of preparation method of effective culture medium for extending lactic acid bacteria survival period according to claim 5, is characterized in that：
The colony density of the saccharomyces cerevisiae is 2 × 105Cfu/g, is saccharomyces cerevisiae to be placed in saccharomyces cerevisiae fluid medium cultivate
Gained, the wherein composition of saccharomyces cerevisiae fluid medium are 0.5 part of peptone, 1 part of glucose, 0.3 part of yeast extract, Fructus Hordei Germinatus
0.3 part of juice.
8. a kind of preparation method of effective culture medium for extending lactic acid bacteria survival period according to claim 5, is characterized in that：
The autoclaving of step (6) is concretely comprised the following steps：Treat steam pressure for 1.05kg/cm2-1.15kg/cm2When keep constant voltage, treat water steam
When the temperature of gas rises to 120 DEG C -122 DEG C, keep sterilizing 18-22 minute under constant temperature, stop pressurization, release after pressure is reduced to 0
Discharge water steam.
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Cited By (7)
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| CN107354097A (en) * | 2017-08-10 | 2017-11-17 | 青岛云溯源健康科技有限公司 | A kind of lactic acid bacteria preservative agent for improving liquid lactic acid bacterium survival rate at normal temperatures and its application |
| CN108130298A (en) * | 2018-01-25 | 2018-06-08 | 广州大学 | A kind of lactic acid bacteria low temperature protects bacterium culture medium and preparation method thereof, application method and application |
| CN108865922A (en) * | 2018-05-02 | 2018-11-23 | 山东出入境检验检疫局检验检疫技术中心 | listeria monocytogenes standard sample and preparation method thereof |
| CN110066734A (en) * | 2019-05-20 | 2019-07-30 | 广州天适立农生态农业发展有限公司 | A kind of quickly breeding biological tropina culture medium |
| CN112063573A (en) * | 2020-08-27 | 2020-12-11 | 贵州茅台酒股份有限公司 | Formula and culture method of lactobacillus isolation medium |
| CN113388521A (en) * | 2021-05-27 | 2021-09-14 | 沈阳博阳饲料股份有限公司 | Diluent for delaying drop of viable count of liquid lactobacillus at normal temperature and preparation method thereof |
| CN113774004A (en) * | 2021-11-11 | 2021-12-10 | 山东国力生物技术研究院 | Lactobacillus brevis and method for preparing gamma-aminobutyric acid by recycling whole cells of lactobacillus brevis |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107354097A (en) * | 2017-08-10 | 2017-11-17 | 青岛云溯源健康科技有限公司 | A kind of lactic acid bacteria preservative agent for improving liquid lactic acid bacterium survival rate at normal temperatures and its application |
| CN107354097B (en) * | 2017-08-10 | 2020-08-11 | 青岛云溯源健康科技有限公司 | Lactobacillus preservative for improving survival rate of liquid lactobacillus at normal temperature and application thereof |
| CN108130298A (en) * | 2018-01-25 | 2018-06-08 | 广州大学 | A kind of lactic acid bacteria low temperature protects bacterium culture medium and preparation method thereof, application method and application |
| CN108865922A (en) * | 2018-05-02 | 2018-11-23 | 山东出入境检验检疫局检验检疫技术中心 | listeria monocytogenes standard sample and preparation method thereof |
| CN110066734A (en) * | 2019-05-20 | 2019-07-30 | 广州天适立农生态农业发展有限公司 | A kind of quickly breeding biological tropina culture medium |
| CN112063573A (en) * | 2020-08-27 | 2020-12-11 | 贵州茅台酒股份有限公司 | Formula and culture method of lactobacillus isolation medium |
| CN113388521A (en) * | 2021-05-27 | 2021-09-14 | 沈阳博阳饲料股份有限公司 | Diluent for delaying drop of viable count of liquid lactobacillus at normal temperature and preparation method thereof |
| CN113774004A (en) * | 2021-11-11 | 2021-12-10 | 山东国力生物技术研究院 | Lactobacillus brevis and method for preparing gamma-aminobutyric acid by recycling whole cells of lactobacillus brevis |
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