CN107356568A - A kind of detection method by principle of fluorescent quenching quick detection saccharification hemoglobin content - Google Patents
A kind of detection method by principle of fluorescent quenching quick detection saccharification hemoglobin content Download PDFInfo
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- CN107356568A CN107356568A CN201710411406.2A CN201710411406A CN107356568A CN 107356568 A CN107356568 A CN 107356568A CN 201710411406 A CN201710411406 A CN 201710411406A CN 107356568 A CN107356568 A CN 107356568A
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- detection
- glycosylated hemoglobin
- detection method
- hemoglobin
- fluorescent quenching
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6432—Quenching
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- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Optics & Photonics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
A kind of new glycosylated hemoglobin detection method, fluorescent quenching standard measure detect the technical principle of glycosylated hemoglobin.Invention broadly provides detection method and technical principle.Glycolated hemoglobin analysis A1C YouPan and glycosylated hemoglobin detection kit based on this technical research（Fluorimetric Quenching Method）It is used cooperatively, for the clinical glycosylated hemoglobin for quantitatively detecting diabetic（HbA1c）, it can also be used to the monitoring of normal person and diabetes risk crowd.
Description
Technical field
The present invention relates to the detection method of the affine glycosylated hemoglobin fluorescent quenching of boric acid, belong to biochemistry quantitative analysis
Category.
Background technology
Glycosylated hemoglobin（HbA1c）It is the product that endoerythrocytic hemoglobin is combined with blood glucose in blood of human body.Blood
The combination generation glycosylated hemoglobin of sugar and hemoglobin is irreversible reaction, and directly proportional to blood sugar concentration, and can keep 120
It or so, so glycosylated hemoglobin detection can generally reflect patient's glycemic control situation of nearly 8 ~ 12 weeks.International diabetes
The new edition Asia-Pacific Guidelines for Management of Diabetes Mellitus that alliance releases, clear stipulaties glycosylated hemoglobin are internationally recognized diabetes monitoring
" goldstandard ".
The method of detection glycosylated hemoglobin has kind more than 20 at present, and main method has three kinds：Ion exchange chromatography, parent
With chromatography and immunization.This detection method is improved on the basis of affinity chromatography, and eosin fluorescence is coupled from boric acid
Material, fluorescent quenching is caused after boric acid combination glycosylated hemoglobin, and detection is quenched signal and contained to quantitative determine glycosylated hemoglobin
Amount, therefore it is Fluorimetric Quenching Method.This method is simple to operate, automated analysis, and accuracy in detection is high, is not easy to be disturbed material shadow
Ring, and be POCT（point-of-care testing）Miniature precision instrument, be easy to clinical practice.
The content of the invention
It is an object of the invention to provide a kind of principle by the affine fluorescent quenching of boric acid can be used for quickly detecting saccharification blood
The detection technique of Lactoferrin content.
In order to realize the above-mentioned purpose of the present invention, the invention provides following technical scheme：
（1）The selection of fluorescent material, eosin（Also known as Yihong）It is a kind of oxa anthracenes faintly acid organic dyestuff to fluoresce, eosin
Fluorescein due to high transmitting effect, high-fluorescence quantum yield and be widely used in biology, medical domain.Choose daybreak
Red -5- thiocarbamide phenylboric acid triethyl amine salts are as key reaction material.Boric acid can specifically bind glycosylated hemoglobin,
The fluorescence of coupling is quenched simultaneously, according to the rate calculations saccharification hemoglobin content of reduction.
（2）The selection of double beam system：According to the exciting light spectrogram of eosin, 510nm is selected as exciting light.According to fluorescence
Spectrogram, detector is selected to be emitted light detecting signal for 580nm.510nm is selected according to the abosrption spectrogram of hemoglobin simultaneously
With the detection light that 570nm is hemoglobin.
（3）Detection method：The glycosylated hemoglobin produced using the arrow Biotechnology Ltd. of Tianjin three detects examination
Agent box（Fluorimetric Quenching Method）The glycolated hemoglobin analysis A1C-YouPan produced with Tianjin You Pan Bioisystech Co., Ltd
The detecting system of composition is detected.Kit equipped with fluorometric reagent is positioned over instrument optical module LED1 and LED2 light path
On intersection point（Detection is indoor）.After blood sample is added in fluorometric reagent, fluorescent material can be with the glycosylated hemoglobin in blood sample
（HbA1c）With reference to initiation fluorescent quenching.LED1 lamps launch wavelength is 510nm fluorescent excitings, and LED2 lamp launch wavelengths are
570nm transmits detection light.There is a photoelectric sensor respectively in LED1 and LED2 right opposites, photoelectric sensor detection passes through reagent
The light energy size of box, and electric signal is converted optical signals into, electric signal passes through FPGA（Field-Programmable Gate
Array, field programmable gate array）Data can be transmitted to CPU after technical finesse, CPU is calculated by blood sugar concentration transfer algorithm
The glycosylated hemoglobin gone out in patient blood sample accounts for the ratio of total hemoglobin.System can be by the data storage measured in storage
Shown over the display in device and by data.The result that client as needed can measure instrument simultaneously prints, when
Patient data is too many, and during insufficient memory, operating personnel can copy patient data on computer to.
Detection unit is represented with %DCCT：
Content/total hemoglobin × 100% of %DCCT=Glycohemoglobin HbA1c
The advantages of the present invention：
The present invention quantitative determines the principle of glycosylated hemoglobin using fluorescent quenching technology, there is provided Method And Principle.Method operates
Simply, kit is completely enclosed, free from environmental pollution, to operator's safety, without toxic action.Coordinate glycosylated hemoglobin
Analyzer supports the use, and goes out result within 4 minutes, and experimental implementation is simple, reduces the stand-by period of patient, improves the effect of clinical detection
Rate.
Brief description of the drawings
Fig. 1 Fluorimetric Quenching Method light path systems.Kit equipped with fluorometric reagent is positioned over instrument optical module LED1
On the intersection point of LED2 light paths（Detection is indoor）.When blood sample add fluorometric reagent in after, fluorescent material can with blood sample
Glycosylated hemoglobin（HbA1c）With reference to initiation fluorescent quenching.LED1 lamps launch wavelength is 510nm fluorescent excitings, and LED2 lamps are sent out
The a length of 570nm transmissions detection light of ejected wave.There are a photoelectric sensor, photoelectric sensor detection respectively in LED1 and LED2 right opposites
Through the light energy size of kit, and electric signal is converted optical signals into, electric signal passes through FPGA（Field-
Programmable Gate Array, field programmable gate array）Data can be transmitted to CPU after technical finesse, CPU passes through blood
The glycosylated hemoglobin that sugared concentration transfer algorithm is calculated in patient blood sample accounts for the ratio of total hemoglobin.System will can be surveyed
The data storage obtained is shown over the display in memory and by data.Client as needed can measure instrument simultaneously
Result print, when patient data is too many, during insufficient memory, operating personnel can copy patient data to computer
On.
Fig. 2 instrument front elevations.
Fig. 3 detects storehouse figure.
Claims (2)
1. fluorescent quenching standard measure detects the detection method of glycosylated hemoglobin.
2. a kind of principle by the affine fluorescent quenching of boric acid is used for quickly detecting the detection technique of saccharification hemoglobin content, bag
Include fluorescent material, the selection of double light path and detection method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710411406.2A CN107356568A (en) | 2017-06-05 | 2017-06-05 | A kind of detection method by principle of fluorescent quenching quick detection saccharification hemoglobin content |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710411406.2A CN107356568A (en) | 2017-06-05 | 2017-06-05 | A kind of detection method by principle of fluorescent quenching quick detection saccharification hemoglobin content |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107356568A true CN107356568A (en) | 2017-11-17 |
Family
ID=60271779
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710411406.2A Pending CN107356568A (en) | 2017-06-05 | 2017-06-05 | A kind of detection method by principle of fluorescent quenching quick detection saccharification hemoglobin content |
Country Status (1)
| Country | Link |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112067566A (en) * | 2020-08-28 | 2020-12-11 | 南开大学 | Colorimetric sensor for quantitative analysis of glycosylated hemoglobin |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0772779A1 (en) * | 1994-07-27 | 1997-05-14 | Raymond Edwards | Glycated proteins assay |
| CN1531654A (en) * | 2001-04-24 | 2004-09-22 | ������˹-ϣ���¹�˾ | Haemoglobin assay |
| CN101416054A (en) * | 2006-03-31 | 2009-04-22 | 商诊疗有限公司 | Fluorescent assay |
-
2017
- 2017-06-05 CN CN201710411406.2A patent/CN107356568A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0772779A1 (en) * | 1994-07-27 | 1997-05-14 | Raymond Edwards | Glycated proteins assay |
| CN1531654A (en) * | 2001-04-24 | 2004-09-22 | ������˹-ϣ���¹�˾ | Haemoglobin assay |
| CN101416054A (en) * | 2006-03-31 | 2009-04-22 | 商诊疗有限公司 | Fluorescent assay |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112067566A (en) * | 2020-08-28 | 2020-12-11 | 南开大学 | Colorimetric sensor for quantitative analysis of glycosylated hemoglobin |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20171117 |
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| WD01 | Invention patent application deemed withdrawn after publication |