CN107347929A - Fructus gleditsiae prevents and treats the application of honeybee Mites - Google Patents
Fructus gleditsiae prevents and treats the application of honeybee Mites Download PDFInfo
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- CN107347929A CN107347929A CN201710581508.9A CN201710581508A CN107347929A CN 107347929 A CN107347929 A CN 107347929A CN 201710581508 A CN201710581508 A CN 201710581508A CN 107347929 A CN107347929 A CN 107347929A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/12—Powders or granules
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
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- Pest Control & Pesticides (AREA)
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Mites (Tropilaelaps spp.) are the important evil mites of Asia Apidae (Apidae), the invention discloses the application of fructus gleditsiae preventing and treating honeybee Mites, there is provided fructus gleditsiae pulvis and fructus gleditsiae compounding powder and preparation method thereof.Mite killing composition fructus gleditsiae is plant tissue, more environmentally friendly to bee product, pollution-free, to honeybee into honeybee, young honeybee, ovum, queen bee safe；Make that fructus gleditsiae pulvis and fructus gleditsiae compounding powder granularity are thinner, and utilization rate of active components is higher using ultramicro grinding, it is more excellent than the effect of sublimed sulfur in the effect of preventing and treating honeybee Mites.
Description
Technical field
The present invention relates to honeybee diseases prevention and control field, and in particular to fructus gleditsiae prevents and treats the application of honeybee Mites.
Background technology
Mites (Tropilaelaps spp.) are the important evil mites of Asia Apidae (Apidae).Its breed it is fast,
The phoresy phase is short, is a kind of honeybee epizoa bigger than varoa mite (Varroa destructor) harmfulness.Mites are original
Host is apis dorsata (Apis dorsata), but also can alternate hose, infect the apis mellifera of Apidae
(A.Mellifera), black apis dorsata (A.laboriosa) and apis florea (A.Florea).
The domestic most common medicament for being used to prevent and treat honeybee Mites has two classes at present：One kind is powder product, mainly there is liter
Magnificent sulphur powder and compound distillation sulphur powder；One kind is aqua product, mainly there is Amitraz.
Wherein, using distillation sulphur powder and compound distillation sulphur powder preventing and treating Mites, medicinal powder need to be equably sprinkling upon to honeybee road and frame
It Liang Shang, can also be applied directly to cover on sub- spleen, but problems be present：
1) powder granularity is thicker, and utilization ratio of drug is not high；
2) long-term use of, preventive effect can decrease, and pulvis used in amounts increases；
3) there is certain toxic action to honeybee, be mainly manifested in that ovum is not hatched, queen bee does not lay eggs.
Mites are prevented and treated using aqua product Amitraz, all honeybee spleens in beehive need to be proposed that carrying out spraying controls mite one by one,
But problems be present：
1) spray operation is carried out one by one, it is time-consuming；
2) prevention effect is poor relative to powder product；
3) Amitraz is bigger to people's toxicity, and residual can influence bee product quality.European Union has been disabled on honeybee.
Fructus gleditsiae, alias：Chinese honey locust, chicken dwell son, gleditsia sinensis, gleditsia officinalis, tooth soap, black rhinoceros, small soap, eyebrow soap, small Chinese honey locust.Pulse family
Gleditsia plant Chinese honey locust, because of the lopsided small pod for being influenceed and being born by wound etc..Autumn harvests, and removes impurity, dries.In cylinder
Shape, it is slightly flat and bend.Pod is dried in cylinder, slightly flat, crescent is made in bending, long 5~10 centimetres, wide 5~12 millimeters.Surface is purple
Brown or atropurpureus, by canescence pollen, wipe rear glossy, and there is tiny verruca and wire crackle, ventral suture to dash forward
It is in rib ridge shape to rise, and dorsal suture projection is not notable and has brown color longitudinal grin.Tip has the style residue of beak, and base portion has carpopodium residual
Trace.Matter is hard and crisp, frangibility, and section outer layer brown color, middle yellow-white, center is softer, there is pale green or fallow thread
Thing and oblique reticulate pattern, longitudinally split the recess of visible marshalling, rarely found seed.It is smelly micro-, it is irritant, smell its powder and then spray
Sneeze, it is peppery after taste elder generation sweet tea.With individual small full, color it is purple it is black, glossy, without carpopodium, matter is hard, more than meat and glue, section light green person is preferred.
The effect of fructus gleditsiae possesses sensible based on medicinal, washs phlegm, searches wind, desinsection and other effects.Also have been reported that display has to kill
The effect of various pest and disease damages on plant, such as Gleditsia sinensis plant miticide and preparation method thereof (CN 101595896), as preventing and treating
Evil mite on the industrial crops such as fruit tree, vegetables, but for the preventing and treating of honeybee pest and disease damage, especially there is not been reported for the preventing and treating of Mites.
The content of the invention
It is an object of the invention to：The defects of overcoming the existing preventing and treating most common medicament of honeybee Mites to exist, there is provided a kind of
Fructus gleditsiae prevents and treats the application of honeybee Mites, and compared with prior art, this kind of method can reduce deleterious effects of the medicament to honeybee,
The prevention effect of Mites is improved, does not influence the quality of bee product.
The present invention is achieved through the following technical solutions：
The invention provides application of the fructus gleditsiae in honeybee chalcid fly acarid control, mainly provides a kind of fructus gleditsiae pulvis and is used for
Honeybee chalcid fly acarid control.
The preparation method of the fructus gleditsiae pulvis comprises the following steps：
1) just crush：Fructus gleditsiae gleditsia sinensis is added into pulverizer and carries out preliminary crushing；
2) sieve：The fructus gleditsiae gleditsia sinensis just crushed is crossed into 50-70 mesh sieves；
3) ultramicro grinding：The fructus gleditsiae pulvis of powder size D90≤23 micron is obtained by ultramicro grinding.
Present invention also offers a kind of application of fructus gleditsiae compounding powder in honeybee chalcid fly acarid control.
Complex material is included in the fructus gleditsiae compounding powder.
The complex material is the one or more in sublimed sulfur, Fructus meliae toosendan, lilac daphne, Genkwanin.
One of described complex material preferred embodiment is sublimed sulfur.
Fructus gleditsiae mass content is 1~99% in the fructus gleditsiae compounding powder, and the mass content of sublimed sulfur is 99~1%.
One of preferred embodiment of the fructus gleditsiae compounding powder, fructus gleditsiae mass content is in the fructus gleditsiae compounding powder
80~90%, the mass content of sublimed sulfur is 10~20%.
One of preferred embodiment of the fructus gleditsiae compounding powder, fructus gleditsiae mass content is in the fructus gleditsiae compounding powder
90%, the mass content of sublimed sulfur is 10%.
The preparation method of the fructus gleditsiae compounding powder comprises the following steps：
1) just crush：Fructus gleditsiae gleditsia sinensis is added into pulverizer and carries out preliminary crushing；
2) sieve：The fructus gleditsiae gleditsia sinensis just crushed is crossed into 50-70 mesh sieves；
3) mix：Fructus gleditsiae is mixed with complex material, stirred；
4) ultramicro grinding：Under inert gas shielding, the fructus gleditsiae of powder size D90≤23 micron is obtained by ultramicro grinding
Compounding powder.
The invention provides a kind of fructus gleditsiae pulvis and fructus gleditsiae compounding powder answering in honeybee chalcid fly acarid control
With the dispensing mode of the fructus gleditsiae pulvis and fructus gleditsiae compounding powder is Sa Feng roads and grid beam, can also be applied directly to cover
On sub- spleen.
The present invention compared with prior art, has advantages below and beneficial effect：
1) fructus gleditsiae is applied to the excellent effect of preventing and treating honeybee Mites；
2) powder size D90≤23 micron of pulvis, its fine size, utilization rate of active components are high；
3) fructus gleditsiae is used in honeybee chalcid fly acarid control, to honeybee into honeybee, young honeybee, ovum, queen bee safe；
4) mite killing composition fructus gleditsiae is plant tissue, more environmentally friendly to bee product, pollution-free.
Embodiment
All features disclosed in this specification, or disclosed all methods or during the step of, except mutually exclusive
Speciality and/or step beyond, can in any way combine, unless specifically stated otherwise, can be equivalent by other or there is class
Replaced like the alternative features of purpose, i.e. unless specifically stated otherwise, one be each characterized as in a series of equivalent or similar characteristics
Individual embodiment.
Used reagent is mainly as follows in the present invention：Control group acaricide, trade name sublimed sulfur, main component are to rise
Magnificent sulphur (manufacturer is Sichuan Wangshi Bee Health Co., Ltd.).Prevention effect medicament is checked, main component is double first
Amidine (manufacturer is Sichuan Wangshi Bee Health Co., Ltd.).
Embodiment 1：
The preparation of fructus gleditsiae pulvis 1：
1) just crush：Fructus gleditsiae gleditsia sinensis is added into pulverizer and carries out preliminary crushing；
2) sieve：The fructus gleditsiae gleditsia sinensis just crushed is put into 3 layers of electronic shock sieve (50 mesh) sieving；
3) ultramicro grinding：The fructus gleditsiae pulvis 1 of powder size D90≤23 micron is obtained by ultramicro grinding.
Embodiment 2：
The preparation of fructus gleditsiae compounding powder 1：
1) just crush：Fructus gleditsiae gleditsia sinensis is added into pulverizer and carries out preliminary crushing；
2) sieve：The fructus gleditsiae gleditsia sinensis just crushed is put into electronic shock sieve (60 mesh) sieving；
3) mix：Fructus gleditsiae (mass content 99%) is mixed with sublimed sulfur (mass content 1%), stirred；
4) ultramicro grinding：Under inert gas shielding, the fructus gleditsiae of powder size D90≤23 micron is obtained by ultramicro grinding
Compounding powder 1.
Embodiment 3：
The preparation of fructus gleditsiae compounding powder 2：
1) just crush：Fructus gleditsiae gleditsia sinensis is added into pulverizer and carries out preliminary crushing；
2) sieve：The fructus gleditsiae gleditsia sinensis just crushed is crossed into 70 mesh sieves；
3) mix：Fructus gleditsiae (mass content 90%) is mixed with sublimed sulfur (mass content 10%), stirred；
4) ultramicro grinding：Under inert gas shielding, the fructus gleditsiae of powder size D90≤23 micron is obtained by ultramicro grinding
Compounding powder 2.
Embodiment 4：
The preparation of fructus gleditsiae compounding powder 3：
1) just crush：Fructus gleditsiae gleditsia sinensis is added into pulverizer and carries out preliminary crushing；
2) sieve：The fructus gleditsiae gleditsia sinensis just crushed is put into 3 layers of electronic shock sieve (70 mesh) sieving；
3) mix：Fructus gleditsiae (mass content 50%) is mixed with sublimed sulfur (mass content 50%), stirred；
4) ultramicro grinding：Under inert gas shielding, the fructus gleditsiae of powder size D90≤23 micron is obtained by ultramicro grinding
Compounding powder 3.
Embodiment 5：
The preparation of fructus gleditsiae compounding powder 4：
1) just crush：Fructus gleditsiae gleditsia sinensis is added into pulverizer and carries out preliminary crushing；
2) sieve：The fructus gleditsiae gleditsia sinensis just crushed is put into 3 layers of electronic shock sieve (60 mesh) sieving；
3) mix：Fructus gleditsiae (mass content 10%) is mixed with sublimed sulfur (mass content 90%), stirred；
4) ultramicro grinding：Under inert gas shielding, the fructus gleditsiae of powder size D90≤23 micron is obtained by ultramicro grinding
Compounding powder 4.
Embodiment 6：
The preparation of fructus gleditsiae compounding powder 4：
1) just crush：Fructus gleditsiae gleditsia sinensis is added into pulverizer and carries out preliminary crushing；
2) sieve：The fructus gleditsiae gleditsia sinensis just crushed is put into 3 layers of electronic shock sieve (50 mesh) sieving；
3) mix：Fructus gleditsiae (mass content 1%) is mixed with sublimed sulfur (mass content 99%), stirred；
4) ultramicro grinding：Under inert gas shielding, the fructus gleditsiae of powder size D90≤23 micron is obtained by ultramicro grinding
Compounding powder.
Embodiment 7：
According to《Honeybee acaricide evaluating drug effect field test guideline》, to fructus gleditsiae pulvis provided by the invention and
Fructus gleditsiae compounding powder carries out the prevention effect evaluation of Mites.
Test method：
Bee colony is tested to be provided by Sichuan Wangshi Bee Health Co., Ltd.'s experiment bee farm, selection group gesture consistent (6 spleen),
The parasitic horizontal consistent bee colony of Mites is divided into 8 groups, every group 20 groups, respectively test group 1 (fructus gleditsiae pulvis 1), experimental group 2
(fructus gleditsiae compounding powder 1), experimental group 3 (fructus gleditsiae compounding powder 2), experimental group 4 (fructus gleditsiae compounding powder 3), experimental group 5
(fructus gleditsiae compounding powder 4), experimental group 6 (fructus gleditsiae compounding powder 5), positive controls 7 (sublimed sulfur group) and negative control group 8
(not medication), honeybee kind are apis mellifera.
Administrated method：Every medication in 7 days once, a cycle medication three times, uses Amitraz spray honeybee spleen to bee colony after 21 days.
After on-test, daily check collect bottom plate fall mite, before on-test, the 22nd day and the 43rd be preordained primary group (point
Ji Lu ovum, larva, honeybee lid, the quantity of adult honeybee (sufficient frame)), before on-test, the 22nd day and the 43rd day investigation capping
Interior Mites parasitic rate.
Mites parasitic rate in capping：100 capping are chosen out in different sub- spleen regions with tweezers at random out of bee colony,
The bee larva in capping room or pupa are pressed from both sides out, statistics has the capping room number of Mites.
Mites parasitic rate (%)=(the capping room sum for having capping room number/inspection of mite) × 100% in capping
Fall the calculating of mite rate：
Fall mite quantity：Observation once falls mite plate daily after administration, collects and counts mite quantity (a), statistical test terminates
When always fall mite number (∑ a)
Statistics finally falls mite quantity：During off-test, each test group bee colony with excessive Amitraz spray spleen, is united respectively respectively
Meter finally falls mite quantity (b).This quantity (b) plus each bee colony are always fallen into mite number (∑ a), as the total mite number of bee colony, to calculate respectively each
Test group bee colony falls mite rate.
Result of the test：
Fall mite rate result see the table below：
Parasitic rate result see the table below：
| Project | Mean parasitized rate % before medication | 22nd day average parasitic rate % after medication | 43rd day average parasitic rate % after medication |
| Fructus gleditsiae pulvis 1 | 6.3 | 1.2 | 0.8 |
| Fructus gleditsiae compounding powder 1 | 6.2 | 1.3 | 0.8 |
| Fructus gleditsiae compounding powder 2 | 6.0 | 1.2 | 0.7 |
| Fructus gleditsiae compounding powder 3 | 6.2 | 1.4 | 1.0 |
| Fructus gleditsiae compounding powder 4 | 6.2 | 1.5 | 1.1 |
| Fructus gleditsiae compounding powder 5 | 6.1 | 1.6 | 1.2 |
| Sublimed sulfur | 6.2 | 1.7 | 1.3 |
| Negative control | 5.9 | 7.5 | 4.0 |
Group's gesture investigation result see the table below：
Conclusion：
According to《Honeybee acaricide evaluating drug effect field test guideline》, to fructus gleditsiae pulvis provided by the invention and
Fructus gleditsiae compounding powder carry out Mites prevention effect evaluation, as a result for：Fructus gleditsiae pulvis and fructus gleditsiae compounding powder it is flat
Fall mite rate and parasitic rate is superior to sublimed sulfur pulvis, from the point of view of group's gesture situation of bee colony, as a result also superior to sublimed sulfur pulvis.Cause
This, it is good to the prevention effect of honeybee Mites using fructus gleditsiae pulvis and fructus gleditsiae compounding powder, and to honeybee safety.
It is described above, be only presently preferred embodiments of the present invention, any formal limitation not done to the present invention, it is every according to
Any simply modification, the equivalent variations made according to the technical spirit of the present invention to above example, each fall within the protection of the present invention
Scope.
Claims (10)
1. fructus gleditsiae prevents and treats the application of honeybee Mites, it is characterised in that application of the fructus gleditsiae in honeybee chalcid fly acarid control.
2. the application of fructus gleditsiae preventing and treating honeybee Mites according to claim 1, it is characterised in that fructus gleditsiae pulvis is in honey
Application in honeybee chalcid fly acarid control.
3. the application of fructus gleditsiae preventing and treating honeybee Mites according to claim 2, it is characterised in that the fructus gleditsiae pulvis
Preparation method comprise the following steps：
1) just crush：Fructus gleditsiae gleditsia sinensis is added into pulverizer and carries out preliminary crushing；
2) sieve：The fructus gleditsiae gleditsia sinensis just crushed is crossed into 50-70 mesh sieves；
3) ultramicro grinding：The fructus gleditsiae pulvis of powder size D90≤23 micron is obtained by ultramicro grinding.
4. fructus gleditsiae prevents and treats the application of honeybee Mites, it is characterised in that fructus gleditsiae compounding powder is in honeybee chalcid fly acarid control
Application.
5. the application of fructus gleditsiae preventing and treating honeybee Mites according to claim 4, it is characterised in that the fructus gleditsiae compound
Pulvis includes complex material, and the complex material is sublimed sulfur.
6. the application of fructus gleditsiae preventing and treating honeybee Mites according to claim 5, it is characterised in that the fructus gleditsiae compound
Fructus gleditsiae mass content is 1~99% in pulvis, and the mass content of sublimed sulfur is 99~1%.
7. the application of fructus gleditsiae preventing and treating honeybee Mites according to claim 5, it is characterised in that the fructus gleditsiae compound
Fructus gleditsiae mass content is 80~90% in pulvis, and the mass content of sublimed sulfur is 10~20%.
8. the application of fructus gleditsiae preventing and treating honeybee Mites according to claim 5, it is characterised in that the fructus gleditsiae compound
Fructus gleditsiae mass content is 90% in pulvis, and the mass content of sublimed sulfur is 10%.
9. the application of fructus gleditsiae preventing and treating honeybee Mites according to claim 4, it is characterised in that the fructus gleditsiae is answered
The preparation method of square pulvis comprises the following steps：
1) just crush：Fructus gleditsiae gleditsia sinensis is added into pulverizer and carries out preliminary crushing；
2) sieve：The fructus gleditsiae gleditsia sinensis just crushed is crossed into 50-70 mesh sieves；
3) mix：Fructus gleditsiae gleditsia sinensis is mixed with complex material, stirred；
4) ultramicro grinding：Under inert gas shielding, the fructus gleditsiae compound of powder size D90≤23 micron is obtained by ultramicro grinding
Pulvis.
10. the application of the fructus gleditsiae preventing and treating honeybee Mites according to claim 2 or 4, it is characterised in that the pulvis makes
It is Sa Feng roads and grid beam with mode, can be also applied directly to cover on sub- spleen.
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| CN201710581508.9A CN107347929B (en) | 2017-07-17 | 2017-07-17 | Application of honeybee mite and bee mite prevention and treatment by using honeybee honeylocust abnormal fruit soap |
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| CN201710581508.9A CN107347929B (en) | 2017-07-17 | 2017-07-17 | Application of honeybee mite and bee mite prevention and treatment by using honeybee honeylocust abnormal fruit soap |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108157298A (en) * | 2017-12-27 | 2018-06-15 | 中国农业科学院蜜蜂研究所 | A kind of method for detecting honeybee cleaning behavior |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108157298A (en) * | 2017-12-27 | 2018-06-15 | 中国农业科学院蜜蜂研究所 | A kind of method for detecting honeybee cleaning behavior |
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| Publication number | Publication date |
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| CN107347929B (en) | 2020-05-08 |
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