CN107318640B - A method of utilizing the perennial feeding coix lacryma-jobi of wild coix lacryma-jobi breeding - Google Patents
A method of utilizing the perennial feeding coix lacryma-jobi of wild coix lacryma-jobi breeding Download PDFInfo
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- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
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Abstract
The invention discloses a kind of methods using the perennial feeding coix lacryma-jobi of wild coix lacryma-jobi breeding.This method include " in 1 year it is sexual to selected single plant growth and the asexual growth stage specific trait; selected and identified twice " the step of, i.e. in Jinghong City, when annual short-day, in the sexual growth phase of wild coix lacryma-jobi, first time selection is carried out to specific trait, after harvesting seed, in the long-day, quick asexual propagation amplifies group, then carries out second to specific trait and select.The present invention opens wild coix lacryma-jobi utilization of resources new way；Make full use of wild coix lacryma-jobi can sexual propagation can invent two stage selection method for specific single plant the characteristics of vegetative propagation again；In conjunction with the climatic characteristic and short day crop growth-development law of Jinghong City, the back-and-forth method for vegetative growth phase and generative growth phase different target character has been invented.The selection for carrying out objective trait twice in 1 year to same single plant, improves selection pressure and efficiency of selection, accelerates breeding process.
Description
Technical field
The invention belongs to plant variety breeding fields, are related to a kind of side using the perennial feeding coix lacryma-jobi of wild coix lacryma-jobi breeding
Method.
Background technique
Coix lacryma-jobi (Coix Lacryma-jobi L.) is grass family Coix in Plant Taxonomy, also known as careless pearl, river
Paddy, Semen Coicis, maize, jobstears, myotonin etc. originate in one band of China and Southeast Asia, at least 6000~10000 years Chinese
Cultivation domestication history.Traditional Land use systems of coix lacryma-jobi are as health food and medicinal plant, Compendium of Material Medica and " book on Chinese herbal medicine warp
Dredge " etc. have in medicine masterpiece with the record of the diseases such as coix lacryma-jobi treatment eczema, rheumatic arthritis, verruca plana.In recent years, Ren Mencong
The coix seed oil and coixenolide extracted in coix lacryma-jobi, has been used to treating cancer.
Coix is closer with the Zea affiliation where cultivated maize, which is mostly 2 times of bodies, and chromosome 20,
For cross pollinated plant, cultivar and wild species can be simply divided into according to seed morphology, wherein cultivation coix lacryma-jobi is mainly used to process
Myotonin, wild coix lacryma-jobi utilize relatively fewer.Cultivar is mostly life in 1 year, preferably regional in light and heat condition, cradle it is rear renewable,
But reproducibility is poor；Wild species be it is perennial, cradling spring in next year after rear renewable or winter generates new point from base portion
Tiller.The ground such as Yunnan Province of China, Sichuan, Guizhou, Guangxi are one of area of origin of coix lacryma-jobi, wild coix lacryma-jobi resource very abundant, are often distributed
In river bank, the side of a pond, marshland, the soughs such as do not reclaim wasteland, there are form abundant and hereditary variation, with excellent resistance to
Moist and disease resistance.Under natural conditions, wild coix lacryma-jobi is bred by seed and rhizome two ways, and coix lacryma-jobi is 4 plant of carbon, is had
The Basic of Biology of high yield, most tiller is vigorous, and plant is tall and big, and cauline leaf is in great numbers, more in the ground peasant such as Guangxi, Yunnan and Hunan
It is directly utilized using local wild coix lacryma-jobi as excellent forage grass.In addition to this, in ethnic groups such as Liuzhou, In Xishuangbanna of Yunnan
Still there is the habit for picking wild coix seed, root, stem etc. as Chinese medicine in area so far, but has no new using wild coix lacryma-jobi breeding
The research of type herbage.
The southwests such as China Yunnan, Guizhou Province, expensive, river, Chongqing are that subtropical zone moistens monsoon climate.Area's heat resources are abundant, remove
Outside high and cold mountain area, whole year is greater than 10 DEG C of accumulated temperature and reaches 4500-6000 DEG C, and most area is more than frost-free period 300d.Annual precipitation reaches
1000mm；Local area rain hot same season, winter warm up morning in spring, and most area percentage of possible sunshine, hereinafter, for the cloudy center in the whole nation, is especially fitted 35%
Close with trophosome utilize based on perennial forage crops growth.On the other hand, sloping upland area in southwest is big, only Yunnan, Guizhou Province,
River, Yu Si save (city) and are greater than 15 ° of sloping upland gross areas up to 713.0 ten thousand hm2, the 48.0% of such national sloping upland gross area is accounted for,
And have the characteristics that breaking topography, the gradient are steep, soil layer is shallow, soil property is loose, water conservation is poor, fertility is low, at such regional especially suitable kind
Perennial forage grass is planted, soil erosion is reduced, preserves the ecological environment.
Based on this, since two thousand, corn research institute, Sichuan Agricultural University at home and abroad takes the lead in having carried out wild coix lacryma-jobi
The research of the perennial forage grass of breeding.Successively in the area of origin of wild coix lacryma-jobi --- the ground such as Yunnan in China, river, expensive and Laos, Burma,
The countries in Southeast Asia such as Vietnam have carried out the collection of wild coix lacryma-jobi germ plasm resource extensively, arrangement, have saved work, and in sichuan agriculture
University's southern corn breeding base (is located at Yunnan Province, the town Ga Sa, the Jinghong City village Man Guangao), establishes wild coix lacryma-jobi germ plasm resource
Garden, system have carried out the mirror of wild coix lacryma-jobi economical character, reproductive characteristic, genetic characteristics, resistance, feeding speciality and disease and insect resistance
The research of fixed and evaluation etc..It is studied for many years, from the wild late-maturing variant of coix lacryma-jobi resource of portion that Yunnan Province is collected, choosing
Bring out that Comprehensive Traits are excellent, perennial, the higher feeding big Black Hills coix lacryma-jobi of coix lacryma-jobi new varieties of feeding value, and in December, 2016
It is authorized by grass product kind validation board, Sichuan Province.
Summary of the invention
The object of the present invention is to provide a kind of methods using the perennial feeding coix lacryma-jobi of wild coix lacryma-jobi breeding.
Method provided by the present invention using the perennial feeding coix lacryma-jobi of wild coix lacryma-jobi breeding, including " to entering menu in 1 year
The specific trait of strain sexual growth and asexual growth stage, is selected twice and is identified " the step of.
It is described that " specific trait of sexual to selected single plant growth and asexual growth stage, is selected twice and is reflected in 1 year
It is fixed " specifically: in Yunnan Province Jinghong City or place with same weather conditions, under the conditions of annual short-day, wild
The sexual growth phase of coix lacryma-jobi carries out first time selection to specific trait, after harvesting seed, under long-day conditions, and quick nothing
Property expand numerous amplification group, then second is carried out to specific trait and is selected.
Wherein, the specific trait for carrying out being related to when first time selection includes: that seedling-growing time is (first with insemination and emergence simultaneously
Sequentially quantify afterwards), plant growing way (institute's value is multiplied with single plant tiller number to quantify with boot stage plant height), seminal propagation energy
Power (is quantified) with seed production；Carry out the specific trait that described second is related to when selecting include: forage grass production performance (with
" when plant it is long to 1m when, start to cradle, cradle within every 30 days later primary, cradle last time by the end of July " gained adds up fresh grass and produces
Amount is to quantify).
It carries out the first time selection to carry out in annual 8-12 month, the Yunnan Province Jinghong City period is short-day, drought
It is conducive to coix lacryma-jobi sexual propagation in season.By acquisition for the wild coix seed single plant nursery of examination, the single plant that selection emergence is fast, growing way is good,
Roguing will be entered to be packaged with the dedicated whole strain of pollen separation net, the mode of free pollination is selfed purifying in single plant, and identifies selected single plant
Seminal propagation ability, select the strong single plant of seminal propagation ability.
It carries out second of selection to carry out in annual 1-7 month, the Yunnan Province Jinghong City period is long-day, rain
It is conducive to coix lacryma-jobi Vegetative growth in season.Single plant after upper season to be harvested to seed, quick asexual propagation by way of cuttage or dividing the root and stem of certain plants
To 30 plants or so, the strain that forage grass production performance is prominent, resistance to cradling property is strong, reproducibility is good is selected.
When carrying out first time selection, the single plant of " emergence is fast " described in selection is referred specifically to simultaneously after planting by emergence
30% single plant before chronological order ranking.The single plant of " growing way is good " described in selection refers specifically to boot stage plant height and single plant
30% single plant before tiller number multiplication gained numerical ranks.The single plant of " seminal propagation ability is strong " described in selection refers specifically to plant
30% single plant before suboutput ranking.
When carrying out second of selection, " forage grass production performance is prominent, resistance to cradling property is strong, reproducibility is good " described in selection
Strain refer specifically to " when plant it is long to 1m when, start to cradle, cradle within every 30 days later primary, cradle last time by the end of July "
Gained adds up before green forage yield ranking 30% strain.
It in the method, further include the selection to resistance when carrying out selecting for described second；The resistance is resistance to
Moist (is local rainy season when select for described second, rainfall is big, soil since enforcement place is Yunnan Province Jinghong City
Humidity, so can survive and plant its moisture-proof selected is all excellent, so need not be to moisture-proof in practical operation
Carry out additional deliberately selection).
The difference of single plant two stage selection is as follows:
In the method, it can carry out one or many described " to the sexual growth of selected single plant and asexual growth in 1 year
The specific trait in stage, is selected twice and is identified " the step of；Described is repeatedly more than twice.
In the method, when having carried out, described " growth sexual to selected single plant and asexual growth stage is specific in 1 year
Character is selected twice and is identified " the step of after, can also further comprise as needed " using grass yield height " as screen
The step of index, therefrom the highest single plant of selection grass yield or strain carry out breeding.
In an example of the present invention, big Black Hills coix lacryma-jobi is to be selected according to provided by the present invention above using wild coix lacryma-jobi
Educate what the method for perennial feeding coix lacryma-jobi selected.Certainly, the specific selection of big Black Hills coix lacryma-jobi belongs to guarantor of the invention
Protect range.
The specific Breeding Process of big Black Hills coix lacryma-jobi is as follows:
2004: to 176 parts of wild coix lacryma-jobi for being collected in Southwestern China area carry out forms, economical character, genetic characteristics,
The system identifications such as reproductive characteristic and feeding value.It was found that number is a late-maturing variant occur in the wild coix lacryma-jobi group of YY103),
Prominent phenotype is to postpone about 1.5 months in the maturity period, and seed is smaller, and setting percentage is not high, and plant tillering is more, and stalk is red, has outside
White wax coat, root system and nutrient growth are flourishing.
2005-2007: independent isolation implant, free pollination in single plant are dug out into the whole strain of variant, current year harvests seed
14.Receipts, miscegenation whole seed are mixed, free pollination between single plant continues 3 years, harvests 618 seeds altogether.Per every year with it is dedicated every
Off-network isolation, prevents miscellaneous wild coix lacryma-jobi pollen contamination, and only sexual expansion is numerous, without selection.
2008: whole seed single plant nursery emerged 497 plants, the first time selection are carried out to it, according to seedling-growing time
348 single plants are eliminated, 149 single plants are selected in；104 single plants are eliminated according to plant height × tiller number, are selected in 45 single plants；According to kind
Suboutput eliminates 31 single plants, is selected in 14 single plants.To strain is upgraded to after above-mentioned 14 single plant asexual propagations, produced according to fresh grass
Amount carries out second and selects, and eliminates 10 strains, finally retains the high strain of 4 yield.
Prior year: being selected in all seeds of 4 strains by 2009-2012, by strain single plant nursery, is chosen in each strain
Elite seedling selfing carry out overall merit and by after maternal plant asexual propagation.For excellent strain, expands selected range, select altogether
It selects to 8 strains.
2013-2016: it is under strict isolation conditions, the expansion of each strain is numerous, multiple years test is carried out, Herbage harvest is selected
Strain Y85-10 high, Comprehensive Traits are excellent, and tested, demonstrated and mating sapling multiplication technology, cultivation technique research and development, it examines
Surely it is named as big Black Hills coix lacryma-jobi.
It is heretofore described that " specific trait of sexual to selected single plant growth and asexual growth stage, carries out twice in 1 year
Application of the step of selection and identification " in new variety of plant breeding also belongs to protection scope of the present invention.
Wherein, the plant can be gramineae plant.The gramineae plant further can be coix lacryma-jobi platymiscium.The heart of a lotus seed
Job's tears platymiscium concretely coix lacryma-jobi.
The present invention has the innovative point of following several respects:
1, the new way of the wild coix lacryma-jobi utilization of resources is opened
China is one of important area of origin of coix lacryma-jobi, and wild coix lacryma-jobi is resourceful, has a variety of hereditary variation types, mostly
Tiller is vigorous, and plant is tall and big, and cauline leaf is in great numbers, resists a variety of pest and disease damages.On Guangxi, Yunnan and Hunan and other places, occasionally there is peasant wild
Coix lacryma-jobi herds or cradles utilization as weeds.In addition to this, in minority areas such as Liuzhou, In Xishuangbanna of Yunnan, until
Still there is the habit for picking wild coix seed, root, stem etc. as Chinese medicine in the present, there is not yet utilizing the wild novel herbage of coix lacryma-jobi breeding
System research.This research utilizes the wild perennial forage grass of coix lacryma-jobi breeding for the first time, opens the new way of the wild coix lacryma-jobi utilization of resources,
Enrich forage germplasm resources.
2, make full use of wild coix lacryma-jobi can sexual propagation again can vegetative propagation the characteristics of, invented for specific single plant
Two stage selection method
Coix lacryma-jobi is Constantly allogamous plant.Under natural conditions, after pollinating between plant or in strain in coix lacryma-jobi wild population, seed
The landing of autumn maturation, spring next year can sprout into new plant；Meanwhile new tiller can be also grown in old stem base portion, it develops for can
Educate branch.In fact, wild coix lacryma-jobi population is the mixture of sexual propagation and vegetative propagation, for plant a certain in group, lead to
After crossing sexual propagation reservation seed, and it is the consistent group of heredity by this plant of rapid expansion using vegetative propagation, thus may be used
The plant is once selected in sexual growth phase, quick asexual propagation amplifies group after harvesting seed, then carries out primary
Selection is selected and is identified twice to selected single plant in that is, 1 year, this method by wild coix lacryma-jobi can sexual propagation again can be asexual
The characteristics of breeding, organically combines, and in the different stages of development, selects different characters, selects pressure big, effectively increase breeding
The accuracy of process and identification.
3, it in conjunction with the climatic characteristic and short day crop growth-development law of Yunnan Province Jinghong City, has invented raw for nutrition
The selection method in long stage and generative growth phase different target character
Wild coix lacryma-jobi is the perennial short-day plant of cross-pollination, is conducive to its reproductive growth, long day under the conditions of short-day
It is conducive to nutrient growth according to condition.Jinghong City annual 8-12 month is short-day, and the daily sunshine-duration is shortened by length, and dry season is conducive to
Coix lacryma-jobi reproductive growth；Annual 1-7 month is the long-day, and the daily sunshine-duration by short elongated, is conducive to coix lacryma-jobi nutrient growth.For the heart of a lotus seed
The difference of Job's tears vegetative growth phase and generative growth phase Agronomic character is long to speed of emergence, seedling stage in generative growth phase
The objective traits such as gesture, single plant tiller, robustness efficiency of selection is high, produces in vegetative growth phase to vegetative propagation coefficient, forage grass
The objective traits such as performance, resistance to cradling property and reproducibility efficiency of selection is high.
The selection for carrying out objective trait twice in 1 year to same single plant, improves selection pressure and efficiency of selection, accelerates
Breeding process.
Detailed description of the invention
Fig. 1 is seedling nursery.
Fig. 2 is Cloned seedling cutting propagation.
Fig. 3 is that big Black Hills coix lacryma-jobi forage grass produces field.
Specific embodiment
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
Big Black Hills coix lacryma-jobi: it is recorded in " Chinese animal and veterinary report on January 8th, 2017；Sichuan Rural Areas daily paper January 12 in 2017
Day；Shandong Yuan Yuan, Tian Gang, the such as Yu Bing dry assessment of nutritional value Practaculture Science of the big Black Hills coix lacryma-jobi complete stool on growth meat rabbit,
2017,34 (5): 1100-1106 ", the public can obtain from applicant, can only be used to repeat present invention experiment use.
Embodiment 1, the expansion for the seedling that grows directly from seeds are numerous
In Yunnan Province Jinghong City, annual August part, by wild coix seed, after being impregnated 10 days in water, be seeded into 10cm ×
In the seedling-growing container of 10cm size, after planting spills and apply a small amount of phoxim granule, the subterranean pest-insects such as prevention and treatment cutworm.It is long extremely to seedling
" three Ye Yixin " phase moves to crop field, and spacing in the rows and line-spacing are 1m, is planted cell with the dedicated pollen separation net of 200 mesh in boot stage
Strain isolation, guarantees in same cell between coix lacryma-jobi strain and sufficient free pollination in strain, prevents miscellaneous pollen.It is mixed receive district seed (if
The a certain type coix seed quantity being collected into is more, can without the stage expansion it is numerous).
The plantation and screening of embodiment 2, seedling
In Yunnan Province Jinghong City, the prior year is expanded numerous coix seed and impregnated in water 10 days, is seeded by annual August part
In the seedling-growing container of 10cm × 10cm size (Fig. 1), after planting spills and apply a small amount of phoxim granule, the undergrounds such as prevention and treatment cutworm evil
Worm after planting records daily number of emerging, is selected in 30% single plant first emerged.Long to " three Ye Yixin " phase to seedling, transplanting is extremely
Crop field, spacing in the rows and line-spacing are 1m.The tiller number and plant height that each single plant is investigated to boot stage, by plant height and single plant tiller number phase
Multiply, according to each single plant score number, be selected in preceding 30% single plant, single plant is isolated with 200 mesh pollen separation nets for selected single plant
Get up, prevents miscellaneous pollen contamination.Seed is harvested, single plant is calculated and produces grain weight.30% single plant before selected single-strain seed output.
The plantation and screening of embodiment 3, Cloned seedling
In Yunnan Province Jinghong City, stalk cuttage is expanded strain selected on last stage by annual January after harvesting seed
It is numerous.Every section is a seedling, and when cuttage, spacing in the rows and line-spacing are 10cm (Fig. 2), Cloned seedling are planted in cell, zone leader
10m, width 5m, seeding row spacing is 1m, when plant it is long to 1m when, start to cradle, cradle within every 30 days later primary, cradle by the end of July most
It is primary afterwards, calculate the accumulative Herbage harvest of each strain.30% strain before selected accumulative Herbage harvest.
The breeding of embodiment 4, big Black Hills coix lacryma-jobi
One, big Black Hills coix lacryma-jobi Variety Introduction
Flourishing, well developed root system, nutrient growth is especially vigorous.For Clipped plant height up to 4.5m or more, single plant is not effective
Tiller 40 or more, under the conditions of preferable, fresh grass is produced per year up to 10t/ mus, Jinghong high-yield culturing reaches 15.6t/ mus within 2014.
Palatability is good, is suitble to feeding ox, sheep, rabbit, fish etc., complete stool contains crude protein 10.7%, starch 13.5% before blooming, always
Sugared 7.7%, acid detergent fiber 28.6%, neutral detergent fiber 48.4%.Year can cradle 2-3 times, plant 1 time, using 3 years
More than.Moisture-proof is prominent, can tolerate -10 DEG C of low temperature of short time, and entire breeding time is not required to prevent and treat any pest and disease damage, lodging resistance
By force.Can by seed, cuttage, divide 3 kinds of modes of the root and stem of certain plants to breed, breeding coefficient is high.1kg seed can plant 10 mu, and 1 plant of cuttage can plant
1.5 mu；1 plant of point of root and stem of certain plants can divide 20 plants or more.
At present in 10 ground (city) such as Sichuan, Ziyang, Meishan, Guangyuan, Suining, Nanchong, Mianyang and Yunnan scape
5 province's (city) large area plantations such as flood, changde, hunan, enshi and Chongqing.
Two, the breeding of big Black Hills coix lacryma-jobi
Form, economical character, heredity spy were carried out to 176 parts of wild coix lacryma-jobi for being collected in Southwestern China area in (1) 2004 year
The system identifications such as point, reproductive characteristic and feeding value.It was found that number is a late-maturing variation occur in the wild coix lacryma-jobi group of YY103
Strain, prominent phenotype are to postpone about 1.5 months in the maturity period, and seed is smaller, and setting percentage is not high, and plant tillering is more, and stalk is red, outer
There is white wax coat, root system and nutrient growth are flourishing.
Coix lacryma-jobi elite seedling (variant in step (1)) free pollination was obtained into 14 seeds in (2) 2005 years；It 2006 will
14 seed sowings, emerge 10 plants, 10 plants of mixed pollination obtain 112 seeds；112 seeds were sowed in 2007, are emerged 74,
74 plants of mixed pollination obtain 618 seeds.Per being isolated every year with dedicated separation net, prevent miscellaneous wild coix lacryma-jobi pollen contamination, it is only sexual
Expand it is numerous, without selection.
(3) 2008 years, 618 seed single plant nursery are emerged 497 plants, count seedling-growing time and calculates each single plant from out
Seedling is selected in 30% first emergence and growing way is prosperous, reach preceding 30% plant of 5 tillers earliest, i.e., to the number of days for growing to 5 tillers
149 plants, successively compiling is Y1, Y2 ... .Y149, eliminates 348 young plant phase slow growth plant.Boot stage calculates the strain of each selected single plant
Height × tiller number is selected in 30% plant, i.e., 45 plants before score according to each strain score, eliminates 104 low single plant (tables of score
1), by 45 plants in 200 mesh pollen separation nets of boot stage, single plant isolation harvests seed.Seed yield per plant is calculated, menu is entered
Strain highest preceding 30% plant of seed production, eliminates 31 single plants (table 2) of low output by 14 plants.
30% 45 single plants before 1 plant height of table × tiller number score rank
| Single plant number | Y2 | Y3 | Y5 | Y17 | Y19 | Y21 | Y33 | Y39 | Y40 | Y42 |
| Plant height × tiller number | 129 | 143 | 134 | 115 | 121 | 124 | 126 | 143 | 154 | 124 |
| Single plant number | Y44 | Y45 | Y48 | Y49 | Y51 | Y57 | Y59 | Y61 | Y63 | Y64 |
| Plant height × tiller number | 123 | 134 | 123 | 132 | 141 | 125 | 127 | 152 | 133 | 136 |
| Single plant number | Y72 | Y77 | Y80 | Y81 | Y84 | Y85 | Y87 | Y88 | Y89 | Y90 |
| Plant height × tiller number | 132 | 145 | 148 | 151 | 131 | 143 | 144 | 132 | 112 | 132 |
| Single plant number | Y92 | Y93 | Y95 | Y97 | Y109 | Y111 | Y123 | Y129 | Y130 | Y131 |
| Plant height × tiller number | 122 | 120 | 131 | 132 | 122 | 132 | 142 | 134 | 131 | 123 |
| Single plant number | Y131 | Y132 | Y139 | Y141 | Y142 | |||||
| Plant height × tiller number | 123 | 132 | 128 | 137 | 125 |
30% 14 single plants before 2 seed yield per plant score rank of table
| Single plant number | Y3 | Y19 | Y33 | Y45 | Y49 | Y61 | Y85 | Y90 | Y111 | Y131 |
| Seed production (g) | 89.2 | 76.5 | 94.7 | 101.2 | 93.2 | 113.9 | 88.4 | 96.7 | 112.3 | 100.4 |
| Single plant number | Y132 | Y139 | Y141 | Y142 | ||||||
| Seed production (g) | 79.5 | 88.3 | 120.7 | 119.4 |
Whole stalk cuttages expansion of 14 selected single plants of upper stage is numerous, and upgrade to variety comparative test, it is long to 1m to plant
When, start to cradle, cradle within every 30 days later once, cradles last time by the end of July, calculate the accumulative green forage yield of each strain, enter
30% single plant before menu strain Herbage harvest chooses 4 single plants (table 3).
Table 3 is selected in excellent strain variety comparative test green forage yield (t/ mus)
| Single plant number | I cell production | Section II cell production | Section III cell production | It is average | Ranking |
| Y19 | 8.7 | 9.1 | 9.3 | 9.0 | 3 |
| Y49 | 10.2 | 9.7 | 11.3 | 10.4 | 2 |
| Y85 | 11.7 | 12.3 | 11.9 | 12.0 | 1 |
| Y111 | 8.7 | 8.9 | 9.1 | 8.9 | 4 |
(4) 2009-2012 is chosen the seed of 4 selected strains excellent in each strain by strain single plant nursery
Individual plant selfing, and by after maternal plant asexual propagation, carry out overall merit.For excellent strain, expands selected range, choose Y19-
1, Y49-3, Y85-10, Y85-17, Y85-23, Y85-33, Y85-34 and Y111-3 amount to 8 strains (mainly according to each strain
It is the height selection of Herbage harvest, 8 highest single plants of Herbage harvest is selected in 4 strains, asexual propagation is 8 strains).
2013-2016, it is under strict isolation conditions, the expansion of each strain is numerous, multiple years test is carried out, it is high, comprehensive to select Herbage harvest
The excellent strain Y85-10 of character is closed (mainly to be selected according to the average Herbage harvest of 8 strain many years, it is highest to select Herbage harvest
Strain Y85-10) (table 4), and authorized in 2016 by Sichuan Province's grass product kind validation board, it is named as big Black Hills coix lacryma-jobi (figure
3)。
Table 4 is selected in 8 strain 3 years average green forage yields (t/ mus)
| Single plant number | I cell production | Section II cell production | Section III cell production | It is average | Ranking |
| Y19-1 | 9.7 | 9.1 | 8.9 | 9.2 | 6 |
| Y49-3 | 10.2 | 11.6 | 11.4 | 11.1 | 2 |
| Y85-10 | 12.7 | 12.3 | 11.3 | 12.1 | 1 |
| Y85-17 | 8.7 | 8.9 | 9.5 | 9.0 | 4 |
| Y85-23 | 9.7 | 11.2 | 11.6 | 10.8 | 3 |
| Y85-33 | 10.3 | 11.2 | 10.7 | 10.7 | 4 |
| Y85-34 | 11.2 | 12.1 | 8.8 | 10.7 | 4 |
| Y111-3 | 10.5 | 9.9 | 10.8 | 10.4 | 5 |
Claims (2)
1. a kind of method using the perennial feeding coix lacryma-jobi of wild coix lacryma-jobi breeding, including " in 1 year growth sexual to selected single plant and
The specific trait in asexual growth stage, is selected twice and is identified " the step of；
" specific trait of sexual to selected single plant growth and asexual growth stage, is selected twice and is identified in 1 year "
Are as follows: in Yunnan Province Jinghong City or place with same weather conditions, under the conditions of annual short-day, in having for wild coix lacryma-jobi
Property growth phase, to specific trait carry out first time selection, harvest seed after, under long-day conditions, quick asexual propagation is put
Big group, then second is carried out to specific trait and is selected；
The specific trait be related to when first time selection includes: seedling-growing time, plant growing way and seminal propagation ability；Into
Described second of the row specific trait being related to when selecting includes: forage grass production performance；
It carries out the first time selection to carry out in annual 8-12 month, by acquisition for trying wild coix seed single plant nursery, choosing
Select the single plant that emergence is fast, growing way is good, roguing will be entered and packaged with the dedicated whole strain of pollen separation net, in single plant the mode of free pollination from
Purifying is handed over, and identifies the seminal propagation ability of selected single plant, the single plant for selecting seminal propagation ability strong；
It carries out second of selection to carry out in annual 1-7 month, the single plant after upper season to be harvested to seed by cuttage or divides the root and stem of certain plants
Mode carry out asexual propagation, select the strain that forage grass production performance is prominent, resistance to cradling property is strong, reproducibility is good；
When carrying out first time selection, the single plant of " emergence is fast " described in selection is referred to simultaneously after planting by emergence sequencing
30% single plant before ranking；
When carrying out first time selection, the single plant of " growing way is good " described in selection refers to boot stage plant height and single plant tiller number phase
30% single plant before numerical ranks obtained by multiplying；
When carrying out first time selection, before the single plant of " seminal propagation ability is strong " described in selection refers to seed production ranking
30% single plant；
When carrying out second of selection, the strain of " forage grass production performance is prominent, resistance to cradling property is strong, reproducibility is good " described in selection
Refer to " when plant it is long to 1m when, start to cradle, cradle within every 30 days later primary, cradle last time by the end of July " gained is accumulative
30% strain before green forage yield ranking；
It further include the selection to resistance when select for described second；The resistance is moisture-proof.
2. according to the method described in claim 1, it is characterized by: being carried out in the method one or many described " in 1 year
The specific trait of sexual to selected single plant growth and asexual growth stage, is selected twice and is identified " the step of；It is described multiple
For more than twice.
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