CN107287118A - A kind of modular cell culture and transport assembly - Google Patents
A kind of modular cell culture and transport assembly Download PDFInfo
- Publication number
- CN107287118A CN107287118A CN201710723835.3A CN201710723835A CN107287118A CN 107287118 A CN107287118 A CN 107287118A CN 201710723835 A CN201710723835 A CN 201710723835A CN 107287118 A CN107287118 A CN 107287118A
- Authority
- CN
- China
- Prior art keywords
- cell culture
- top cover
- transport assembly
- cell
- culture medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
Abstract
The present invention relates to a kind of modular cell culture and transport assembly, including top cover, connection frame below top cover, bottom below connection frame, ventilated membrane is provided between top cover and connection frame, water permeable membrane is provided between connection frame and bottom, top cover surrounds the air chamber to be formed for storing air with ventilated membrane, ventilated membrane, connection frame and water permeable membrane surround the cell culture chamber to be formed for cultivating cell, water permeable membrane surrounds the Culture Medium Laboratory to be formed for storing nutrient solution with bottom, the side wall of top cover is provided with the air intlet and air outlet slit connected respectively with air chamber, the cell import being respectively communicated with cell culture chamber and cell outlet are provided with the side wall for connecting frame, the culture medium import connected with Culture Medium Laboratory and culture medium outlet are provided with the side wall of bottom, pH sensor is additionally provided with Culture Medium Laboratory.The modular cell culture of the present invention and transport assembly automaticity is high, relatively low cost and cell culture convenience, convenient transport, safely, effectively.
Description
Technical field
The present invention relates to a kind of biometrics, more particularly to a kind of cell modularization culture and transport assembly.
Background technology
With stem cell and immunocyte widely using in therapeutic treatment, standardization, personalized scale cell
Production and transportation turn into one of medical domain development and the critical bottleneck applied.American National cell manufactures association (National
Cell Manufacturing Consortium, NCMC) the relevant scale manufacture announced at it at the beginning of 2016 is various for clinical
Treat and pointed out in the 10 years route maps produced using cell, at present in cell commercial process, from cell production technology,
Preserve, dispatching, each step such as processing all has a series of ground for having much room for improvement and standardizing such as technology, monitoring, data management
Side.And this production process is because the personalized demand for the treatment of becomes more complicated.
There are a variety of commercial automations or semi-automatic cell culture system in the market, wherein majority is only applicable to simultaneously
Carry out mass propgation and the amplification of a few cell product.The demand of personalized treatment is thus not particularly suited for, that is, is measured small and number is more
Situation.For example, being likely at personalized cell industrial production center while carrying out the expansion of hundreds of or thousands of Patient cells
Production, and the demand of each cell culture is less than 5 liters.This monitoring just given in production process, regulation, quality inspection and cost
The problem of bringing a series of unprecedented in terms of control.
In addition, the transport that another big factors that clinical personalization cell therapy is restricted are exactly cell products is asked
Topic.If depositing cell transport using Frozen, its resurrection rate is difficult more than 50%-60%.And the living cells for using existing market to provide
The equipment of transport then can only preserve the cell viability of 24 hours.Because the culture dish for preserving living cells at present is impossible
Ensure in the transportation up to a couple of days as the enough nutritional ingredients of cell supplement and oxygen.Simultaneously current Tissue Culture Dish
The risk being contaminated in transportation is also larger.Therefore, most cell therapies are in hospital or research center progress.
Which limits the promotion and application of cell therapy technology.Simultaneously because technology and the behaviour of each hospital or research institute staff
Differ as level, the quality and effect of cell therapy is hardly resulted in unified and is improved.
Therefore, in view of above-mentioned defect, the design people is actively subject to research and innovation, to found a kind of new structure
Personalized, modular cell culture, management and transport integration component, make it with more the use value in industry.
The content of the invention
In order to solve the above technical problems, it is an object of the invention to provide a kind of automaticity is high, cost is relatively low and cell
Cultivate convenience, convenient transport, safe and effective modular cell culture and transport assembly.
The modular cell culture of the present invention and transport assembly, including top cover, the connection frame below the top cover, position
Bottom below the connection frame, is provided with ventilated membrane, between the connection frame and bottom between the top cover and connection frame
Be provided with water permeable membrane, the top cover and ventilated membrane surround the air chamber to be formed for storing air, the ventilated membrane, connection frame and
Water permeable membrane surrounds the cell culture chamber to be formed for cultivating cell, and the water permeable membrane surrounds to be formed for storing nutrient solution with bottom
Culture Medium Laboratory, the side wall of the top cover is provided with the air intlet and air outlet slit connected respectively with the air chamber, described
The cell import being respectively communicated with the cell culture chamber and cell outlet, the side of the bottom are provided with the side wall for connecting frame
It is provided with wall in the culture medium import connected with the Culture Medium Laboratory and culture medium outlet, the Culture Medium Laboratory and is additionally provided with pH
It is worth sensor.
When it is implemented, the modular cell culture and transport assembly by the import of air, cell and culture medium, go out
Mouth is connected with cell culture maincenter.Each modular cell culture and transport assembly can put connection at any time as desired
With disengaging cental system.
By such scheme, the present invention at least has advantages below：The modular cell culture of the present invention and transport assembly,
Ventilated membrane is provided between air chamber and cell culture chamber, passes through the effect of ventilated membrane so that contains 5% titanium dioxide in air chamber
The air of carbon constantly can carry out gas exchanges with cell culture chamber, so that cellular respiration is used.It is arranged at cell culture chamber
Water permeable membrane between Culture Medium Laboratory can constantly be cultivated the culture medium in Culture Medium Laboratory with cell culture chamber
Base is exchanged, so that cell absorbs.The pH value data of culture medium can be sent to control by the pH sensor set in Culture Medium Laboratory
Center, is controlled so that control centre can change speed to the culture medium in Culture Medium Laboratory, instead of operating personnel
Subjective judgement, the artificial traditional approach for changing liquid, realize numerical control and effectively change liquid, and avoid and manually change what liquid may be brought
Wasting phenomenon, reduces the cost of cell large-scale production, and solves the problems, such as repeatedly to change the cell contamination that liquid is likely to result in.
The problem of modular cell culture and transport assembly of the present invention not only solves cell pilot scale culture, while
The transportation problem of cell is solved, the modular cell culture and transport assembly using the present invention carry out personalized cell scale
Change after culture, modular cell culture and transport assembly can depart from directly from culture systems, and carry out whole Transporting, and nothing
Cell need to be re-started filling, reduce the risk of cell contamination.The setting of ventilated membrane and transparent liquid film simultaneously, being capable of maximum limit
Degree ensures the subsequent need of during transport cell to live gas and culture medium, so as to considerably increase fresh cells
Survival rate, it is ensured that the Clinical practice of sufficient amount cell, and fundamentally eliminate using Frozen deposit cell transport method.
In addition, specific implementation when modular cell culture and transport assembly on can be set identification marking code, such as Quick Response Code,
Which ensure that personalized cell is from clinical acquisitions, the trackability in Clinical practice whole process is transported in culture.Clinician
Observed with the encryption system that patient can not only be provided by the cell production center and search cell production and transportation process, also may be used
To determine the uniqueness and correctness of cell products by distinguishing mark code.
In summary, modular cell culture of the invention and transport assembly, automaticity is high, cost is relatively low and cell
Convenient transport, safely, effectively.
Further, modular cell culture of the invention and transport assembly, between the top cover and connection frame, connect frame
Sealing ring is respectively arranged between bottom.
Sealing ring can effectively prevent the leakage of air or culture medium, in addition to sealing ring, can also pass through other encapsulants
Or modular cell culture and transport assembly are effectively sealed using special technique (such as ultrasonic sealing).
Further, modular cell culture of the invention and transport assembly, the top cover, connection frame and bottom are vertical
It is square.
This, which is designed, causes modular cell culture and transport assembly are easy to stack to set, so that operating personnel being capable of profit
Fairly large culture systems are assembled into multiple modular cell culture and transport assembly, while the design also causes modular
Cell culture and transport assembly are readily transported.
Further, modular cell culture of the invention and transport assembly, the upper and lower surface of the connection frame
On be respectively equipped with slot, the lower surface of the top cover and the upper surface of bottom and be respectively arranged with the positioning being adapted to the slot
Plate.
The purpose of the design is to allow the operator to top cover, connection frame and bottom are accurately aligned with and connected.
During specific works, operating personnel by location-plate alignment and insert location-plate in slot.After connection, top cover, bottom
With the surface of connection frame height can be kept to flush.
Further, modular cell culture of the invention and transport assembly, the left and right sides of the bottom is set respectively
There is connecting plate, the bottom of the connecting plate is hinged on the lateral surface of bottom, the top of connecting plate and the top cover are removably
Connection.
The setting of connecting plate so that operating personnel are connected top cover and bottom by connecting plate, so that modular is thin
Born of the same parents cultivate and transport assembly is in transportation, difficult for drop-off between top cover, connection frame and bottom, and operating personnel can be overall
Modular cell culture and transport assembly are moved, the efficiency of transport is added.
Further, it is fixed to be provided with modular cell culture of the invention and transport assembly, the lateral surface of the top cover
The clamping screw being threadedly coupled with the locating slot is provided with position groove, the connecting plate.
The design realizes being detachably connected for connecting plate and top cover, and when specifically used, operating personnel can be by clamping screw
It is connected in insertion locating slot and with top cover by screw thread.
Further, it is hinged with modular cell culture of the invention and transport assembly, the lateral surface of the connecting plate
The first in command.
The setting of the first in command so that operating personnel can move modular cell culture and transport group by the first in command
Part, operation is very easy.
Further, is hinged with modular cell culture of the invention and transport assembly, the upper surface of the top cover
Second chief.
The setting of the second in command allows the operator to carry modular cell culture and transport assembly by it.
Further, modular cell culture of the invention and transport assembly, in addition to base, the surface of the base is set
There is mounting groove, the bottom of the bottom is located in the mounting groove, and heating resistance wire, institute are arranged on the bottom surface of the mounting groove
State and graphite film is provided with above heating resistance wire, the bottom is made from a material that be thermally conductive.
The purpose of the design is so that operating personnel can be entered when temperature is relatively low by heating resistance wire to culture medium
Row heating, complies with predetermined temperature.During specific works, the heat that heating resistance wire is produced is conducted the bottom of to by graphite film
Cover and the culture medium in Culture Medium Laboratory is heated.
Further, temperature is additionally provided with modular cell culture of the invention and transport assembly, the Culture Medium Laboratory
Sensor.
The setting of temperature sensor enable control centre have a try grasp Culture Medium Laboratory in temperature.
Described above is only the general introduction of technical solution of the present invention, in order to better understand the technological means of the present invention,
And can be practiced according to the content of specification, below with presently preferred embodiments of the present invention and coordinate accompanying drawing describe in detail as after.
Brief description of the drawings
Fig. 1 is modular cell culture and the structural representation of transport assembly of the present invention；
Fig. 2 is modular cell culture and the structural representation of transport assembly of embodiment one；
Fig. 3 is the partial enlarged drawing in A portions in Fig. 2；
Fig. 4 is the structured flowchart of high-density cells production system；
Fig. 5 is modular cell culture and the structural representation of transport assembly of embodiment two；
Fig. 6 is the partial enlarged drawing in B portions in Fig. 5.
In figure, 1：Top cover；2：Connect frame；3：Bottom；4：Ventilated membrane；5：Water permeable membrane；6：Air chamber；7：Cell culture chamber；
8：Culture Medium Laboratory；9：Air intlet；10：Air outlet slit；11：Cell import；12：Cell outlet；13：Culture medium import；14：Training
Support base outlet；15：PH value sensor；16：Sealing ring；17：Location-plate；18：Connecting plate；19：Locating slot；20：Clamping screw；
21：The first in command；22：The second in command；23：Base；24：Mounting groove；25：Heating resistance wire；26：Graphite film；27：TEMP
Device.
Embodiment
With reference to the accompanying drawings and examples, the embodiment to the present invention is described in further detail.Implement below
Example is used to illustrate the present invention, but is not limited to the scope of the present invention.
Embodiment one：
Referring to Fig. 1 to Fig. 4, a kind of modular cell culture of a preferred embodiment of the present invention and transport assembly, including top
Lid 1, the connection frame 2 below top cover, the bottom 3 below connection frame, top cover are the box like structure that Open Side Down, connection
Frame is rectangular box, and bottom is the box like structure of opening upwards, and ventilated membrane 4 is provided between top cover and connection frame, ventilated membrane
Edge is fixedly installed on the medial surface of top cover, and water permeable membrane 5 is provided between connection frame and bottom, and the edge of water permeable membrane, which is fixed, to be set
Put on the medial surface of bottom, top cover and ventilated membrane surround the air chamber 6 to be formed for storing air, ventilated membrane, connection frame and
Water permeable membrane surrounds the cell culture chamber 7 to be formed for cultivating cell, and water permeable membrane surrounds to be formed for storing nutrient solution with bottom
Culture Medium Laboratory 8, the side wall of top cover is provided with the air intlet 9 and air outlet slit 10 that are connected respectively with air chamber, connects the side of frame
Be provided with wall on the cell import 11 being respectively communicated with cell culture chamber and cell outlet 12, the side wall of bottom be provided with
PH sensor 15 is additionally provided with the culture medium import 13 and culture medium outlet 14 of Culture Medium Laboratory connection, Culture Medium Laboratory.Top cover
Connection frame between, connection frame and bottom between be respectively arranged with sealing ring 16.Top cover, connection frame and bottom are cube knot
Structure.
The modular cell culture of the embodiment and transport assembly, Culture Medium Laboratory are carried out by water permeable membrane and cell culture chamber
Culture medium is exchanged.Ensure the freshness of culture medium in cell culture chamber.Culture medium freshness in Culture Medium Laboratory mainly passes through
Built-in pH sensor is monitored.After its pH changes more than predetermined value, system is supplemented by excluding old culture medium
New culture medium is adjusted.
Modular cell culture and transport assembly can form high-density cells production system by the method for superposition, high
Density cells production system is regulated and controled by central control system and distribution system.This set causes composition high-density cells life
Each unit of production system can produce the cell of Different Individual.Reach the large-scale production of individuation product.
Wherein, control centre and distribution system mainly have two functions：1st, control enters composition (such as dioxy of system gas
Change concentration of carbon), and it is distributed to the air-flow velocity of each gas compartment (A).2nd, the pH information sent from each Culture Medium Laboratory is received.
The speed to each Culture Medium Laboratory offer fresh culture is determined accordingly.And according to feedback, regulations speed.
Reaching the unit of raw cell culture target can take out directly from cell production systems.Inflate and train by air chamber
Foster basal cell changes directly packaging express delivery after liquid.Due to there is quite sufficient air and fresh culture.Sample can transported
The vigor of cell is kept in journey.So as to solve key issue of the cell products from production to transport difficult.
In summary, the setting of ventilated membrane so that the air containing carbon dioxide in air chamber can be continuously
Cell culture chamber is delivered to, so that cellular respiration is used.The water permeable membrane being arranged between cell culture chamber and Culture Medium Laboratory can
Culture medium in Culture Medium Laboratory is continuously delivered in cell culture room, so that cell absorbs.In Culture Medium Laboratory
The pH value data of culture medium can be sent to control centre by the pH sensor of setting, so that control centre can be to culture
Base transporting velocity is controlled, and be instead of the subjective judgement of operating personnel, the artificial traditional approach for changing liquid, is realized and at the uniform velocity change
Liquid, and the artificial pollution problem for changing liquid band is avoided, reduce the cost of cell large-scale production.The modular of the present invention
The problem of cell culture and transport assembly not only solve cell pilot scale culture, while the transportation problem of cell is also solved,
Directly it can be transported after cell pilot scale culture by modular cell culture and transport assembly, without cell is entered
Row is filling, reduces the risk of cell contamination.The setting of ventilated membrane, can ensure the process in transport to greatest extent simultaneously
Middle cell adds the survival rate of cell, reduces cost to the demand of gas.
Embodiment two：
Referring to Fig. 5 and Fig. 6, modular cell culture and transport assembly structure basic phase of the embodiment with embodiment one
Together, it connects to be respectively equipped with slot, the lower surface of top cover and the upper surface of bottom in the upper and lower surface of frame and set respectively
It is equipped with the location-plate 17 being adapted to slot.The left and right sides of bottom is respectively arranged with connecting plate 18, and the bottom of connecting plate is hinged on
On the lateral surface of bottom, the top of connecting plate and top cover are detachably connected.Locating slot 19 is provided with the lateral surface of top cover, even
The clamping screw 20 being threadedly coupled with locating slot is provided with fishplate bar.The first in command 21 is hinged with the lateral surface of connecting plate.Top
The second in command 22 is hinged with the upper surface of lid.Also include base 23, the surface of base is provided with mounting groove 24, the bottom position of bottom
In in mounting groove, it is arranged on the bottom surface of mounting groove above heating resistance wire 25, heating resistance wire and is provided with graphite film 26, bottom
Lid is made from a material that be thermally conductive.Temperature sensor 27 is additionally provided with Culture Medium Laboratory.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of spirit or essential attributes of the invention, those skilled in the art can realize this in other specific forms
Invention.Therefore, no matter from the point of view of which point, embodiment all should be regarded as exemplary, and is nonrestrictive, the present invention
Protection domain limited by appended claims rather than described above.
In addition, the above is only the preferred embodiment of the present invention, it is not intended to limit the invention, it is noted that for this
For the those of ordinary skill of technical field, without departing from the technical principles of the invention, some improvement can also be made
And modification, these improvement and modification also should be regarded as protection scope of the present invention.Although it will also be appreciated that this specification according to
Embodiment is been described by, but not each embodiment is only comprising an independent technical scheme, this narration of specification
Mode is only that for clarity, the skilled in the art should refer to the specification as a whole, the technology in each embodiment
Scheme may also be suitably combined to form that other embodiments which can be understood by those skilled in the art.
Claims (10)
1. a kind of modular cell culture and transport assembly, it is characterised in that：Connection including top cover, below the top cover
Frame, the bottom below the connection frame, ventilated membrane, the connection frame and bottom are provided between the top cover and connection frame
Between be provided with water permeable membrane, the top cover surrounds the air chamber to be formed for storing air, the ventilated membrane, connection with ventilated membrane
Frame and water permeable membrane surround the cell culture chamber to be formed for cultivating cell, and the water permeable membrane surrounds to be formed for storing training with bottom
The Culture Medium Laboratory of nutrient solution, the side wall of the top cover is provided with the air intlet and air outlet slit that are connected respectively with the air chamber,
The cell import being respectively communicated with the cell culture chamber and cell outlet, the bottom are provided with the side wall of the connection frame
Side wall on be provided with the culture medium import connected with the Culture Medium Laboratory and culture medium outlet, also set up in the Culture Medium Laboratory
There is pH sensor.
2. modular cell culture according to claim 1 and transport assembly, it is characterised in that：The top cover and connection frame
Between, connection frame and bottom between be respectively arranged with sealing ring.
3. modular cell culture according to claim 1 and transport assembly, it is characterised in that：The top cover, connection frame
It is cube with bottom, the surface of top cover is provided with identification code, and the identification code is Quick Response Code.
4. modular cell culture according to claim 1 and transport assembly, it is characterised in that：The upper table of the connection frame
It is respectively equipped with slot, the lower surface of the top cover and the upper surface of bottom and is respectively arranged with and the slot on face and lower surface
The location-plate of adaptation.
5. modular cell culture according to claim 1 and transport assembly, it is characterised in that：The left and right two of the bottom
Side is respectively arranged with connecting plate, and the bottom of the connecting plate is hinged on the lateral surface of bottom, the top of connecting plate and the top
Lid is detachably connected.
6. modular cell culture according to claim 5 and transport assembly, it is characterised in that：The lateral surface of the top cover
On be provided with locating slot, the connecting plate and be provided with the clamping screw being threadedly coupled with the locating slot.
7. modular cell culture according to claim 6 and transport assembly, it is characterised in that：The outside of the connecting plate
The first in command is hinged with face.
8. modular cell culture according to claim 7 and transport assembly, it is characterised in that：The upper surface of the top cover
On be hinged with the second in command.
9. modular cell culture according to claim 1 and transport assembly, it is characterised in that：Also include base, it is described
The surface of base is provided with mounting groove, and the bottom of the bottom is located in the mounting groove, is arranged on the bottom surface of the mounting groove
Graphite film is provided with above heating resistance wire, the heating resistance wire, the bottom is made from a material that be thermally conductive.
10. modular cell culture according to claim 9 and transport assembly, it is characterised in that：In the Culture Medium Laboratory
It is additionally provided with temperature sensor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710723835.3A CN107287118A (en) | 2017-08-22 | 2017-08-22 | A kind of modular cell culture and transport assembly |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710723835.3A CN107287118A (en) | 2017-08-22 | 2017-08-22 | A kind of modular cell culture and transport assembly |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107287118A true CN107287118A (en) | 2017-10-24 |
Family
ID=60106243
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710723835.3A Withdrawn CN107287118A (en) | 2017-08-22 | 2017-08-22 | A kind of modular cell culture and transport assembly |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107287118A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5707869A (en) * | 1994-06-28 | 1998-01-13 | Wolf; Martin L. | Compartmentalized multiple well tissue culture plate |
| CN1290744A (en) * | 1999-12-09 | 2001-04-11 | 中国科学院力学研究所 | Spacial dialyzing counterflow cell cultivator and cultivating method |
| US6228607B1 (en) * | 1995-04-28 | 2001-05-08 | Organogenesis Inc. | Bioreactor |
| US6329195B1 (en) * | 1998-08-03 | 2001-12-11 | Acm-Biotech Gmbh | Cell culture apparatus |
| CN101611133A (en) * | 2006-12-07 | 2009-12-23 | 威尔森沃尔夫制造公司 | The method of high-performance device and culturing cell |
-
2017
- 2017-08-22 CN CN201710723835.3A patent/CN107287118A/en not_active Withdrawn
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5707869A (en) * | 1994-06-28 | 1998-01-13 | Wolf; Martin L. | Compartmentalized multiple well tissue culture plate |
| US6228607B1 (en) * | 1995-04-28 | 2001-05-08 | Organogenesis Inc. | Bioreactor |
| US6329195B1 (en) * | 1998-08-03 | 2001-12-11 | Acm-Biotech Gmbh | Cell culture apparatus |
| CN1290744A (en) * | 1999-12-09 | 2001-04-11 | 中国科学院力学研究所 | Spacial dialyzing counterflow cell cultivator and cultivating method |
| CN101611133A (en) * | 2006-12-07 | 2009-12-23 | 威尔森沃尔夫制造公司 | The method of high-performance device and culturing cell |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP2980200B1 (en) | Cell culturing device, culturing vessel, and holding vessel | |
| CN102803462B (en) | Personal box for collection preparation, collection preparation system, and collection preparation method | |
| US20040168341A1 (en) | Methods for performing operations, a housing for such methods, and furnishings for such housing | |
| CN205874455U (en) | Indirect culture apparatus of cell | |
| CN108611271A (en) | Totally enclosed type intelligent biology production system and production method | |
| WO2015076391A1 (en) | Automatic culture system and cell management system | |
| KR101727231B1 (en) | Automatically controlled growing conditions and plant nutrient solution supply plant cultivating device | |
| CN206274925U (en) | Plant incubator | |
| CN104603257A (en) | Solid state bioreactor adapted for automation | |
| CN208055361U (en) | A kind of multi-functional cell incubator | |
| CN104805012A (en) | Automatic cell culture box | |
| KR101075032B1 (en) | Cell cultivation device and cell cultivation apparatus comprising the same | |
| CN207109018U (en) | A kind of modular cell culture and transport assembly | |
| US20170145365A1 (en) | Cell culturing device and closed-system culture vessel | |
| CN107287118A (en) | A kind of modular cell culture and transport assembly | |
| CN212013989U (en) | Miniature plant tissue culture case | |
| CN110129199A (en) | A kind of three-dimensional artificial culture systems of stem cell | |
| CN208883905U (en) | The adjustable cell culture apparatus of medical experiment oxygen-supplying amount | |
| CN108641953A (en) | Totally enclosed type intelligent biology production system and production method | |
| CN206069890U (en) | A kind of resistant to pollution CO2 gas incubator | |
| EP3048164B1 (en) | Incubator | |
| JP6514952B2 (en) | Automatic culture device | |
| TWI834133B (en) | cell culture system | |
| CN103190288A (en) | Opening lifting cultivating and fruiting technology for vertical bag cultivation for edible fungi | |
| CN212077079U (en) | Cell culture bottle |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20171024 |
|
| WW01 | Invention patent application withdrawn after publication |