CN107287115A - A kind of denitrification magnetotactic bacteria is separately cultured device - Google Patents
A kind of denitrification magnetotactic bacteria is separately cultured device Download PDFInfo
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- CN107287115A CN107287115A CN201710683058.4A CN201710683058A CN107287115A CN 107287115 A CN107287115 A CN 107287115A CN 201710683058 A CN201710683058 A CN 201710683058A CN 107287115 A CN107287115 A CN 107287115A
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- 241000894006 Bacteria Species 0.000 title claims abstract description 44
- 230000000721 bacterilogical effect Effects 0.000 claims abstract description 28
- 238000007599 discharging Methods 0.000 claims description 11
- 230000001651 autotrophic effect Effects 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 238000005273 aeration Methods 0.000 claims 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 8
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 abstract description 4
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 abstract description 4
- 238000006243 chemical reaction Methods 0.000 abstract description 4
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 4
- 238000002474 experimental method Methods 0.000 abstract description 2
- 239000003795 chemical substances by application Substances 0.000 description 9
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 229910052760 oxygen Inorganic materials 0.000 description 6
- 239000001301 oxygen Substances 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 239000000203 mixture Substances 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 239000010802 sludge Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- SZVJSHCCFOBDDC-UHFFFAOYSA-N ferrosoferric oxide Chemical compound O=[Fe]O[Fe]O[Fe]=O SZVJSHCCFOBDDC-UHFFFAOYSA-N 0.000 description 2
- 210000002244 magnetosome Anatomy 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 235000001968 nicotinic acid Nutrition 0.000 description 2
- 239000010865 sewage Substances 0.000 description 2
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 239000006249 magnetic particle Substances 0.000 description 1
- 230000005111 magnetotaxis Effects 0.000 description 1
- 229910052960 marcasite Inorganic materials 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000007483 microbial process Effects 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 229910052683 pyrite Inorganic materials 0.000 description 1
- NIFIFKQPDTWWGU-UHFFFAOYSA-N pyrite Chemical compound [Fe+2].[S-][S-] NIFIFKQPDTWWGU-UHFFFAOYSA-N 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000012549 training Methods 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
- C02F11/04—Anaerobic treatment; Production of methane by such processes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
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Abstract
Device is separately cultured the invention provides a kind of denitrification magnetotactic bacteria, including denitrification case, magnetic separating device and bacteriological incubator, denitrification case is located at the top of magnetic separating device, bacteriological incubator is located at magnetic separating device bottom, wherein denitrification case, magnetic separating device and bacteriological incubator are connected by neck, detachable to use.Apparatus of the present invention can improve nitrate nitrogen and nitrite nitrogen is converted into the conversion ratio of gaseous nitrogen, and integrated denitrification, denitrification magnetotactic bacteria separate and be incubated at one, easy to use, are easy to experiment to use.
Description
Technical field
The invention belongs to water-treatment technology field, and in particular to a kind of denitrification magnetotactic bacteria is separately cultured device and its made
Use method.
Background technology
Completing the method for denitrification process has many kinds, many based on microbial process at present, because nitrifier is autotrophy
Aerobic microbiological, and denitrifying bacterium mostly be heterotrophism anaerobe, so in sewage disposal generally by control dissolved oxygen and
Regulation carbon source preferably realizes denitrification process.In theory when carbon-nitrogen ratio is more than 5-10:1 side can realize denitrification process, and
For high concentration N class sewage, the problem of carbon-nitrogen ratio is too low is all there is, additional carbon is needed in order to complete denitrification process.
Magnetotactic bacteria(Magnetotactic bacterium)It is that a class can make directed movement simultaneously in the presence of external magnetic field
Nano magnetic particle-magnetic corpusculum is formed in vivo(Magnetosome)Bacterium, it is distributed mainly on soil, lake and ocean
Deng in sludge underwater.There is magnetotactic bacteria magnetotaxis to be due to containing magnetic corpusculum(Magnetosomes), different magnetotactic bacterias conjunctions
Into the composition of magnetic corpusculum, morphosis, quantity and size it is different.Composition has Fe3O4、FeS、Fe3S4Or FeS2, magnetic is small
Body is coated by one layer of biomembrane, thickness 10nm of film or so.The research of magnetotactic bacteria has to biomagnetism and bionics Study
Significance.Magnetotactic bacteria due to have the advantages that it is simple in construction, largely exist in nature, it is most likely that be used as a kind of mould
Formula material, for understanding the function and mechanism of that there is magnetic-particle in organism;Or bionics Study provides theoretical foundation,
Make it to be simulated and copied in engineering technology, particularly it can also produce response to trickle earth's magnetic field, so as to
It is applied in the high-technology field such as navigation and orientation.Therefore, the ability that magnetotactic bacterium migrates along the earth's magnetic field magnetic line of force can be made
Influence of the earth's magnetic field to biosystem is studied for a kind of pattern.
The content of the invention
Device is separately cultured it is an object of the invention to provide a kind of denitrification magnetotactic bacteria, for prior art, this hair
Bright device can improve nitrate nitrogen and nitrite nitrogen is converted into the conversion ratio of gaseous nitrogen, while having the function of separation and culture.
The present invention is realized especially by following technical scheme.
A kind of denitrification magnetotactic bacteria is separately cultured device, including denitrification case, magnetic separating device and bacteriological incubator, institute
The denitrification case stated is located at the top of magnetic separating device, and described bacteriological incubator is located at magnetic separating device bottom, wherein anti-nitre
Change case, magnetic separating device and bacteriological incubator to connect by neck, it is detachable to use.Described denitrification case top surface and bottom surface cloth
Full aperture, denitrification case is built with denitrfying agent.Described magnetic separating device upper end is provided with first charging aperture, and bottom is provided with
First discharging opening, magnetic field generator is provided with the respective side wall of magnetic separating device, and the wall of described magnetic field generator is set
There is collecting board.Described Bacteria Culture box top is provided with second charging aperture, and described bacteriological incubator bottom sets temperature control
Device processed, described Bacteria Culture box top is provided with oxygen environment bascule.
Described denitrfying agent is autotrophic denitrification agent, and denitrfying agent is porous sintered solid content.
Described bacteriological incubator surface of internal cavity is additionally provided with temperature sensor, described temperature sensor and temperature control
Device is connected by temperature-control circuit.
Described magnetic separating device and the front and rear wall of bacteriological incubator are provided with the solarization air cap for being passed through inner chamber, described exposure
Stomata is used to connect air generator, and described solarization air cap is turned off or on by valve.
When installing magnetic separating device and bacteriological incubator, the first described discharging opening is in mutual with second charging aperture
The position matched somebody with somebody.
Wall is provided with division board before and after described magnetic separating device, and the front face of described bottom division board is provided with
Three discharging openings.
The corresponding wall of described Bacteria Culture box cavity is provided with multiple mutual concordant protruding brackets, and user places training
Support platform.
Beneficial effects of the present invention:
Device is separately cultured the invention provides a kind of denitrification magnetotactic bacteria, the work with denitrification and separation magnetotactic bacteria
With operationally when water or sludge containing magnetotactic bacteria can improve nitrate nitrogen by denitrification case and nitrite nitrogen is converted into
The conversion ratio of gaseous nitrogen, then separates magnetotactic bacteria by magnetic separating device, and magnetotactic bacteria enters bacteriological incubator after collection
Cultivated, apparatus structure is simple, it is convenient, it is easy to experiment to use.
Brief description of the drawings
Fig. 1 is the structural representation of apparatus of the present invention;
Fig. 2 is the structural representation of the side view of magnetic separating device 3 of the present invention.
In figure:1st, denitrification case, 2, first charging aperture, 3, magnetic separating device, 4, magnetic field generator, 5, collecting board, 6, every
From plate, the 7, first discharging opening, 8, second charging aperture, 9, oxygen environment bascule, 10, bacteriological incubator, 11, cultivation platform, 12,
Temperature control equipment, 13, solarization air cap, the 14, second discharging opening.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
As depicted in figs. 1 and 2, a kind of denitrification magnetotactic bacteria is separately cultured device, including denitrification case 1, Magneto separate dress
3 and bacteriological incubator 10 are put, denitrification case 1 is located at the top of magnetic separating device 3, and bacteriological incubator 10 is located at magnetic separating device 3
Bottom, wherein denitrification case 1, magnetic separating device 3 and bacteriological incubator 10 are connected by neck, are easy to that connection is used and fractionation makes
With.
It is preferred that, the top surface of denitrification case 1 and bottom surface are covered with aperture, and denitrification case is built with denitrfying agent, and denitrfying agent can
Think the autotrophic denitrification agent of the compositions such as sulphur, calcium carbonate, and denitrfying agent is porous sintered solid content.
It is preferred that, the upper end of magnetic separating device 3 is provided with first charging aperture 2, and bottom is provided with the first discharging opening 7, in magnetic point
Respective side wall from device 3 is provided with magnetic field generator 4, and the wall of magnetic field generator 4 is provided with collecting board 5, in bottommost
The front face of division board 6 is additionally provided with the second discharging opening 14.
It is preferred that, the top of bacteriological incubator 10 is provided with second charging aperture 8, and the bottom of bacteriological incubator 10 sets temperature control
Device 12, the top of bacteriological incubator 10 sets aerobic environment bascule 9.The surface of internal cavity of bacteriological incubator 10 is additionally provided with temperature
Sensor, temperature sensor is connected with temperature control equipment 12 by temperature-control circuit.The correspondence of the inner chamber of bacteriological incubator 10
Wall is provided with multiple mutual concordant protruding brackets, and user places cultivation platform 11.
It is furthermore preferred that the front and rear wall of magnetic separating device 3 and bacteriological incubator 10 is provided with the solarization air cap 13 for being passed through inner chamber,
Solarization air cap 13 is used to connect air generator, and solarization air cap 13 is turned off or on by valve, when installing magnetic separating device 3 and thin
Bacterium incubator 10, the first discharging opening 7 is in the position being mutually matched with second charging aperture 8.
When in use, water or sludge containing magnetotactic bacteria are entered the present apparatus by the top of denitrification case 1, in denitrfying agent
Under effect, improve the conversion ratio that nitrate nitrogen and nitrite nitrogen are converted into gaseous nitrogen, then enter Magneto separate by first charging aperture 2
Device 3, starts external air generator and magnetic field generator 4, carries out the separation of magnetotactic bacteria, thin by the effect magnetotactic in magnetic field
Bacterium is adsorbed in the collecting board 5 on magnetic field generator 4, is then shut off magnetic field generator 4, magnetotactic bacteria comes off by second
Charging aperture 8 enters in bacteriological incubator 10, controls the temperature of bacteriological incubator 10 by the temperature control equipment 12 set in advance, carefully
The oxygen environment bascule 9 that the top of bacterium incubator 10 is set automatically adjusts the suitable oxygen that oxygen concentration makes to meet denitrification magnetotactic bacteria
Environment.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of changes, modification can be carried out to these embodiments, replace without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (8)
1. a kind of denitrification magnetotactic bacteria is separately cultured device, including denitrification case(1), magnetic separating device(3)And Bacteria Culture
Case(10), it is characterised in that:Described denitrification case(1)Positioned at magnetic separating device(3)Top, described bacteriological incubator
(10)Positioned at magnetic separating device(3)Bottom, wherein denitrification case(1), magnetic separating device(3)And bacteriological incubator(10)Pass through card
Groove is connected, described denitrification case(1)Top surface and bottom surface are covered with aperture, and denitrification case is built with denitrfying agent;Described magnetic point
From device(3)Upper end is provided with first charging aperture(2), bottom is provided with the first discharging opening(7), in magnetic separating device(3)Pair
Answer side wall that magnetic field generator is installed(4), described magnetic field generator(4)Wall be provided with collecting board(5);Described bacterium
Incubator(10)Top is provided with second charging aperture(8), described bacteriological incubator(10)Bottom sets temperature control equipment
(12), described bacteriological incubator(10)Top sets aerobic environment bascule(9).
2. a kind of denitrification magnetotactic bacteria according to claim 1 is separately cultured device, it is characterised in that:Described anti-nitre
Agent is autotrophic denitrification agent, and denitrfying agent is porous sintered solid content.
3. a kind of denitrification magnetotactic bacteria according to claim 1 is separately cultured device, it is characterised in that:Described bacterium
Incubator(10)Surface of internal cavity is additionally provided with temperature sensor, temperature sensor and temperature control equipment(12)Pass through temperature control
Circuit is connected.
4. a kind of denitrification magnetotactic bacteria according to claim 1 is separately cultured device, it is characterised in that:Described magnetic point
From device(3)And bacteriological incubator(10)Front and rear wall be provided with the solarization air cap for being passed through inner chamber(13).
5. a kind of denitrification magnetotactic bacteria according to claim 4 is separately cultured device, it is characterised in that:Described aeration
Hole(13)For connecting air generator, solarization air cap(13)Turned off or on by valve.
6. a kind of denitrification magnetotactic bacteria according to claim 1 is separately cultured device, it is characterised in that:Described first
Discharging opening(7)With second charging aperture(8)In the position being mutually matched.
7. a kind of denitrification magnetotactic bacteria according to claim 1 is separately cultured device, it is characterised in that:Described magnetic point
From device(3)Front and rear wall is provided with division board(6), described bottommost division board(6)Front face be provided with the 3rd discharging
Mouthful.
8. a kind of denitrification magnetotactic bacteria according to claim 1 is separately cultured device, it is characterised in that:Described bacterium
Incubator(10)The corresponding wall of inner chamber is provided with multiple mutual concordant protruding brackets, and user places cultivation platform(11).
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108441421A (en) * | 2018-04-02 | 2018-08-24 | 郭庆 | A kind of magnetotactic bacteria culture instrument |
CN108504577A (en) * | 2018-04-08 | 2018-09-07 | 郭庆 | A kind of soil bacteria screening technique |
CN108504550A (en) * | 2018-04-08 | 2018-09-07 | 郭庆 | A kind of soil bacteria screening installation |
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JPS6261599A (en) * | 1985-09-13 | 1987-03-18 | Tdk Corp | Collection of magnetotactic bacteria and collecting tool |
JPS63248384A (en) * | 1987-04-02 | 1988-10-14 | Tdk Corp | Separation of magnetotactic bacteria |
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CN203530328U (en) * | 2013-09-27 | 2014-04-09 | 东北林业大学 | Device of separating and collecting magnetotactic bacteria and magnetosomes |
CN104986870A (en) * | 2015-07-10 | 2015-10-21 | 浙江大学 | Rice planting water heavy-metal pollution recycling treatment apparatus and control method |
US20150336828A1 (en) * | 2014-05-23 | 2015-11-26 | Nch Corporation | Method for Improving Quality of Aquaculture Pond Water |
CN207330951U (en) * | 2017-08-11 | 2018-05-08 | 兰州理工大学 | A kind of denitrification magnetotactic bacteria is separately cultured device |
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2017
- 2017-08-11 CN CN201710683058.4A patent/CN107287115A/en active Pending
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JPS6261599A (en) * | 1985-09-13 | 1987-03-18 | Tdk Corp | Collection of magnetotactic bacteria and collecting tool |
JPS63248384A (en) * | 1987-04-02 | 1988-10-14 | Tdk Corp | Separation of magnetotactic bacteria |
JP2002346593A (en) * | 2001-05-22 | 2002-12-03 | Kurita Water Ind Ltd | Method and apparatus for denitrification |
CN101844831A (en) * | 2009-03-25 | 2010-09-29 | 株式会社日立工业设备技术 | Method of wastewater treatment and wastewater treatment equipment |
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CN101671631A (en) * | 2009-10-22 | 2010-03-17 | 中南大学 | Magnetic bacteria separometer |
CN203530328U (en) * | 2013-09-27 | 2014-04-09 | 东北林业大学 | Device of separating and collecting magnetotactic bacteria and magnetosomes |
US20150336828A1 (en) * | 2014-05-23 | 2015-11-26 | Nch Corporation | Method for Improving Quality of Aquaculture Pond Water |
CN104986870A (en) * | 2015-07-10 | 2015-10-21 | 浙江大学 | Rice planting water heavy-metal pollution recycling treatment apparatus and control method |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108441421A (en) * | 2018-04-02 | 2018-08-24 | 郭庆 | A kind of magnetotactic bacteria culture instrument |
CN108504577A (en) * | 2018-04-08 | 2018-09-07 | 郭庆 | A kind of soil bacteria screening technique |
CN108504550A (en) * | 2018-04-08 | 2018-09-07 | 郭庆 | A kind of soil bacteria screening installation |
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Application publication date: 20171024 |