CN203530328U - Device of separating and collecting magnetotactic bacteria and magnetosomes - Google Patents
Device of separating and collecting magnetotactic bacteria and magnetosomes Download PDFInfo
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- CN203530328U CN203530328U CN201320598658.8U CN201320598658U CN203530328U CN 203530328 U CN203530328 U CN 203530328U CN 201320598658 U CN201320598658 U CN 201320598658U CN 203530328 U CN203530328 U CN 203530328U
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Abstract
The utility model relates to a device of separating and collecting magnetotactic bacteria and magnetosomes. A feed inlet is arranged at the upper end of the device, a discharge port is arranged at the bottom of the device, stirring equipment is further arranged at the bottom of the device, a collecting plate for collecting thallus and magnetosomes is arranged on the inner side wall of the device, magnetic filed generators are symmetrically arranged on the outer side wall, and an ultrasonic probe is arranged in the position of the feed inlet. Under a state of not starting ultrasonic waves, culture liquor containing magnetotactic bacteria can adsorb thallus to the collecting plate in the stirring process by means of a magnetic field effect. Once ultrasonic waves are started, thallus of magnetotactic bacteria can be ultrasonically crushed to separate magnetosomes and thallus. Under the effect of an externally applied magnetic field, magnetosomes are adsorbed to the collecting plate, so that the magnetosomes are collected. The device is simple in structure, low in cost and convenient to use, and provides the optimal means for advanced research on separation of magnetotactic bacteria and magnetosomes.
Description
Technical field
The present invention utilizes magnetic field effect and hyperacoustic broken wall effect to collect the thalline of magnetotactic bacteria and magnetic corpusculum, belongs to microbe separation technology field.
Background technology
Magnetotactic bacteria (Magnetotactic bacteria) is the gram negative bacterium that a class can be moved along field direction, widely distributed, in lake, ocean, even in wet soil, can find.That find the earliest magnetotactic bacteria is the doctor of medicine Salvatore Bellini of institute of microbiology of Italian Pavia university.1958, during his pathogenic bacteria in detecting water sample, chanced on the magnetotaxis of bacterium.1975, first American Blakemore Bellini has reported magnetotactic bacteria on < < Science > >, when he is the spirobacteria in research marine bottom mud, surprisingly found that a bacterioid is always gathered in the Northern Margin that leans on of field of microscope drop.By further research, confirm that this bacterioid direction of motion can change along with the change of externally-applied magnetic field polarity, he names this bacterioid into magnetotactic bacteria.Subsequently, scientist even detects or is separated to polytype magnetotactic bacteria soil from the Southern Hemisphere, the Northern Hemisphere and equatorial seawater, lake, pond, marsh in succession.Up to now, by nearly more than 40 strains of magnetotactic bacteria of the method researchs such as 16SrRNA sequencing, fluorescent mark, unicellular horizontal in situ hybridization, the magnetotactic bacteria of having found has coccus, bacillus, vibrios, spirobacteria etc.
It is owing to containing magnetic corpusculum (Magnetosomes) that magnetotactic bacteria has magnetotaxis, and the composition of the magnetic corpusculum that different magnetotactic bacterias are synthetic, morphological structure, quantity and size are different.Composition has Fe
3o
4, FeS, Fe
3s4 or FeS
2, magnetic corpusculum is coated by one deck microbial film, the thickness 10nm left and right of film.The form of magnetic corpusculum has truncated octahedra shape, parallelepiped-shaped, bullet shaped or arrow shaped, teardrop shaped, tooth shape etc.Magnetic corpusculum comprises a bar magnetic corpusculum chain at the most, and what have only has 1～2 magnetic corpusculum, and the even generation having, up to 1000 crystallizations, is arranged in as 5 ropes and forms tube bank.The size of magnetic corpusculum because of bacterial classification different, magnitude range 20～100nm.
Magnetic corpusculum is a kind of bacterium that heavy metal is had to very strong adsorptivity.Some heavy metals can also be absorbed by magnetotactic bacteria and for self breeding as iron, nickel etc.Since being found, the external research that has just started to utilize magnetotactic bacteria processing heavy metal wastewater thereby, as Bahaj of University of Southampton etc. has studied the active impact of different heavy metal ion on magnetotactic bacteria, shown that Cu, Co, Cd, Zn plasma energy suppress the activity of magnetotactic bacteria, Fe, Mg, Mn, Al, Cr etc. can be by the conclusions of active adsorption and removal, so can be used to process waste water undetermined.Research discovery, magnetotactic bacteria is very high to the removal efficiency of above-mentioned ion, as a rule can be by mass concentration by (10～100) * 10
-6mg/L drops to (10～100) * 10
-9mg/L, well below international emission standard, and utilizing magnetotactic bacteria to process in the process of heavy metal, as long as allow wastewater streams cross a fixing magnetic field, just can remove heavy metal, does not need extra power, also does not need to add other medicine.Therefore, have advantages of that economically additive method is incomparable, especially, at the society of energy scarcity, along with the enhancing of people's awareness of saving energy, this method has bright application prospect.
The research of magnetotactic bacteria is significant to biomagnetism and bionics Study.Magnetotactic bacteria is simple in structure owing to having, in advantages such as a large amount of existence of occurring in nature, very likely as a kind of type material, be used for understanding the function and mechanism of that has magnetic-particle in organism.Also can be bionics Study theoretical foundation is provided, make it in engineering, simulated and copy, particularly it also can produce response to trickle terrestrial magnetic field, thereby can in the high-technology fields such as navigation and orientation, be applied.Therefore the ability that, magnetotactic bacterium moves along terrestrial magnetic field magnetic line of force can be used as a kind of pattern and studies the impact of terrestrial magnetic field on biosystem.
Bacterial magnetic particles is compared many advantages with general artificial nano-carrier: 1. have very narrow particle size distribution and stable crystalline form; 2. its nanoscale makes it have huge specific surface area, can fix a large amount of biologically active substances; 3. particle principal constituent is Fe3O4 etc., without other impurity, has paramagnetism; 4. crystal grain has adipose membrane coated, is difficult for assembling; 5. on Magnetosome membrane with a large amount of bio-active groups, can be used for covalently bound with drug molecule, and connect after medicine can be by externally-applied magnetic field and easily separated purifying; 6. medicine carrying magnetic corpusculum can be realized drug release by degraded magnetic corpusculum adventitia in vivo.Because of but a kind of important nano material has many potential using values at biological and medical field.
In application medically, mainly comprise: manufacture magnetic cell and magnetic separation technique, biosensor technique and detection, use magnetic corpusculum as the carrier of targeted nano medicine, tumor thermotherapy, gene diagnosis and therapy, for MRI image dose etc.
The formation of magnetotactic bacteria magnetic corpusculum and magnetic property research, also can contribute to point out biomineralization process, inquires into the mechanism that biological response terrestrial magnetic field changes, and is also the important research content of archaeomagnetism, rock magnetism and environmental magnetism.
The applied research of magnetotactic bacteria is more and more subject to people and payes attention to, at high-technology fields such as microbiology, biological chemistry, biophysics, petrifactology, medical science, computer and aviations, all show more potential using value, therefore the separation of magnetotactic bacteria and being gathered into as important step of magnetic corpusculum, the utility model provides the equipment of a kind of separated and collected magnetotactic bacteria and magnetic corpusculum, this apparatus structure is simple, with low cost, easy to use, effect is remarkable, for the separation of magnetotactic bacteria and magnetic corpusculum, furthers investigate best means is provided.
Utility model content
A device for separated and collected magnetotactic bacteria and magnetic corpusculum, by forming with lower member: there is opening for feed (5) device upper end, and bottom of device is provided with discharge port (6), and discharge port (6) is provided with valve, can free switch; Bottom of device is also provided with whipping device (4), and the inner side-wall of device has collecting board (3), and the outer side wall corresponding with collecting board is arranged with magnetic field generator (2), and opening for feed is partly provided with ultrasound probe (1);
Wherein, the stirring velocity of whipping device is by stirring governor control; Hyperacoustic energy size is by ultrasonic wave
Projector regulates; Collecting board can freely be dismantled.
Described ultrasound probe (1) can be selected by union lever (7) fixing, also can adopt external stationary installation to suspend.
Described magnetic field generator is a kind of in magnet or Helmholtz coil.
Described microorganism collection plate faces respectively magnetic field generator, and the axis of collecting board is consistent with magnetic line of force direction.
Two described Helmholtz coils are parallel to be arranged relatively and vertically.
The magnetic field size that described Helmholtz coil produces, regulates by controller.
After dress liquid, the surface of liquid is higher than collecting board and magnetic field generator.
Hyperacoustic energy magnitude range is 1～2000W.
Following 1～the 5cm of ultrasound probe submergence liquid level after dress liquid.
The utility model equipment is not being opened under hyperacoustic state, and the nutrient solution that contains magnetotactic bacteria can utilize magnetic field effect that thalline is adsorbed onto on collecting board in whipping process, plays the effect of collecting thalline; Open ultrasonic wave, can be by the thalline ultrasonication of magnetotactic bacteria, magnetic corpusculum is separated with thalline, and under additional the action of a magnetic field, magnetic corpusculum will be adsorbed on collecting board, play the effect of collecting magnetic corpusculum.This apparatus structure is simple, with low cost, easy to use, for the separation of magnetotactic bacteria and magnetic corpusculum, furthers investigate best equipment is provided.
Accompanying drawing explanation
Below in conjunction with accompanying drawing and embodiment, the utility model is further described.
The device schematic diagram of Fig. 1 separated and collected magnetotactic bacteria and magnetic corpusculum
1: ultrasonic probe; 2: magnetic field generator; 3: collecting board; 4: whipping device; 5 opening for feeds; 6: discharge port; 7: union lever
Embodiment
Device and the using method thereof of 1 one kinds of separated and collected magnetotactic bacterias of embodiment and magnetic corpusculum
There is opening for feed the device upper end of separated and collected magnetotactic bacteria and magnetic corpusculum, and bottom of device is provided with discharge port, and discharge port is provided with valve, can free switch; Bottom of device is also provided with whipping device, and the inner side-wall of device has microorganism collection plate, and outer side wall is arranged with Helmholtz coil, and opening for feed is partly provided with ultrasound probe.
Magnetotactic bacteria M.gryphiswaldense MSR-1 by cultivating, joins equipment from opening for feed, opens whipping appts, at the uniform velocity stir 10min, open Helmholtz coil, between milli tesla of controlling magnetic field intensity 0.5Hao tesla to 2, continue slowly to stir 10h, stop stirring, bacterium liquid is emitted from discharge port, take out collecting board, thalline is rinsed and reclaimed, close Helmholtz coil controller, close general supply.
Device and the using method thereof of 2 one kinds of separated and collected magnetotactic bacterias of embodiment and magnetic corpusculum
There is opening for feed the device upper end of separated and collected magnetotactic bacteria and magnetic corpusculum, and bottom of device is provided with discharge port, and discharge port is provided with valve, can free switch; Bottom of device is also provided with whipping device, and the inner side-wall of device has microorganism collection plate, and outer side wall is arranged with magnet.
By containing the river of magnetotactic bacteria, from opening for feed, join equipment, open whipping appts, batch type 24h, stops stirring, and bacterium liquid is emitted from discharge port, takes out collecting board, and thalline is rinsed and reclaimed, and closes general supply.
Device and the using method thereof of 3 one kinds of separated and collected magnetotactic bacterias of embodiment and magnetic corpusculum
There is opening for feed the device upper end of separated and collected magnetotactic bacteria and magnetic corpusculum, and bottom of device is provided with discharge port, and discharge port is provided with valve, can free switch; Bottom of device is also provided with whipping device, and the inner side-wall of device has microorganism collection plate, and outer side wall is arranged with Helmholtz coil, and opening for feed is partly provided with ultrasound probe, and ultrasound probe is fixed on equipment by union lever, but can freely pull down.
By the magnetotactic bacteria M.gryphiswaldense MSR-1 cultivating, from opening for feed, join equipment, open whipping appts, at the uniform velocity stir 30min, open ultrasonic device, control ultrasonic energy is 500W, and ultrasonic 5h under batch type condition opens Helmholtz coil, controlling magnetic field intensity is between 1 milli tesla of milli tesla to 2, continue to stir 5h, stop ultrasonic, stop stirring, bacterium liquid is emitted from discharge port, take out collecting board, magnetic corpusculum is rinsed and reclaimed, close Helmholtz coil controller, close general supply.
Finally it should be noted that: the foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although the present invention is had been described in detail with reference to previous embodiment, for a person skilled in the art, its technical scheme that still can record aforementioned each embodiment is modified, or part technical characterictic is wherein equal to replacement.Within the spirit and principles in the present invention all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (9)
1. a device for separated and collected magnetotactic bacteria and magnetic corpusculum, is characterized in that: there is opening for feed (5) device upper end, and bottom of device is provided with discharge port (6), and discharge port (6) is provided with valve, can free switch; Bottom of device is also provided with whipping device (4), and the inner side-wall of device has collecting board (3), and the outer side wall corresponding with collecting board is arranged with magnetic field generator (2), and opening for feed position is provided with ultrasound probe (1); Wherein, the stirring velocity of whipping device is by stirring governor control; Hyperacoustic energy size is regulated by ultrasonic transmitter; Collecting board can freely be dismantled.
2. the device of separated and collected magnetotactic bacteria as claimed in claim 1 and magnetic corpusculum, is characterized in that: described ultrasound probe (1) can be selected by union lever (7) fixing, also can adopt external stationary installation to suspend.
3. the device of separated and collected magnetotactic bacteria as claimed in claim 1 and magnetic corpusculum, is characterized in that: described magnetic field generator is a kind of in magnet or Helmholtz coil.
4. the device of separated and collected magnetotactic bacteria as claimed in claim 1 or 2 and magnetic corpusculum, is characterized in that: microorganism collection plate faces respectively magnetic field generator, and the axis of collecting board is consistent with magnetic line of force direction.
5. the device of separated and collected magnetotactic bacteria as claimed in claim 1 or 2 and magnetic corpusculum, is characterized in that: after dress liquid, the surface of liquid is higher than collecting board and magnetic field generator.
6. the device of separated and collected magnetotactic bacteria as claimed in claim 3 and magnetic corpusculum, is characterized in that: two Helmholtz coils are parallel to be arranged relatively and vertically.
7. the device of separated and collected magnetotactic bacteria as claimed in claim 3 and magnetic corpusculum, is characterized in that: the magnetic field size that described Helmholtz coil produces, regulates by controller.
8. the device of separated and collected magnetotactic bacteria as claimed in claim 7 and magnetic corpusculum, is characterized in that: hyperacoustic energy magnitude range is 1～2000W.
9. the device of separated and collected magnetotactic bacteria as claimed in claim 1 or 2 and magnetic corpusculum, is characterized in that: the following 1～5cm of ultrasound probe submergence liquid level after dress liquid.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320598658.8U CN203530328U (en) | 2013-09-27 | 2013-09-27 | Device of separating and collecting magnetotactic bacteria and magnetosomes |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320598658.8U CN203530328U (en) | 2013-09-27 | 2013-09-27 | Device of separating and collecting magnetotactic bacteria and magnetosomes |
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| CN203530328U true CN203530328U (en) | 2014-04-09 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104986870A (en) * | 2015-07-10 | 2015-10-21 | 浙江大学 | Rice planting water heavy-metal pollution recycling treatment apparatus and control method |
| CN105193733A (en) * | 2014-06-09 | 2015-12-30 | 东北林业大学 | Medicine slow-release nanoparticle wrapping magnetosomes of magnetotactic bacteria and preparation method of medicine slow-release nanoparticle |
| CN107287115A (en) * | 2017-08-11 | 2017-10-24 | 兰州理工大学 | A kind of denitrification magnetotactic bacteria is separately cultured device |
| WO2018127102A1 (en) * | 2017-01-04 | 2018-07-12 | 南京金斯瑞生物科技有限公司 | Automatic purification system and biological sample purification method |
| CN117247836A (en) * | 2023-11-20 | 2023-12-19 | 山东省国土空间数据和遥感技术研究院(山东省海域动态监视监测中心) | Device and method for collecting different magnetotactic bacteria in ocean in layered manner |
-
2013
- 2013-09-27 CN CN201320598658.8U patent/CN203530328U/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105193733A (en) * | 2014-06-09 | 2015-12-30 | 东北林业大学 | Medicine slow-release nanoparticle wrapping magnetosomes of magnetotactic bacteria and preparation method of medicine slow-release nanoparticle |
| CN104986870A (en) * | 2015-07-10 | 2015-10-21 | 浙江大学 | Rice planting water heavy-metal pollution recycling treatment apparatus and control method |
| WO2018127102A1 (en) * | 2017-01-04 | 2018-07-12 | 南京金斯瑞生物科技有限公司 | Automatic purification system and biological sample purification method |
| US11254906B2 (en) | 2017-01-04 | 2022-02-22 | Nanjing GenScript Biotech Co., Ltd. | Automatic purification system and biological sample purification method |
| CN107287115A (en) * | 2017-08-11 | 2017-10-24 | 兰州理工大学 | A kind of denitrification magnetotactic bacteria is separately cultured device |
| CN117247836A (en) * | 2023-11-20 | 2023-12-19 | 山东省国土空间数据和遥感技术研究院(山东省海域动态监视监测中心) | Device and method for collecting different magnetotactic bacteria in ocean in layered manner |
| CN117247836B (en) * | 2023-11-20 | 2024-02-02 | 山东省国土空间数据和遥感技术研究院(山东省海域动态监视监测中心) | Device and method for collecting different magnetotactic bacteria in ocean in layered manner |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140409 Termination date: 20160927 |
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| CF01 | Termination of patent right due to non-payment of annual fee |