CN1072799C - Sensitive method for quickly testing microbe pollution by electroluminescence and its use - Google Patents
Sensitive method for quickly testing microbe pollution by electroluminescence and its use Download PDFInfo
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- CN1072799C CN1072799C CN 96105260 CN96105260A CN1072799C CN 1072799 C CN1072799 C CN 1072799C CN 96105260 CN96105260 CN 96105260 CN 96105260 A CN96105260 A CN 96105260A CN 1072799 C CN1072799 C CN 1072799C
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- 238000000034 method Methods 0.000 title claims abstract description 53
- 238000012360 testing method Methods 0.000 title claims description 43
- 238000005401 electroluminescence Methods 0.000 title claims description 16
- 230000000813 microbial effect Effects 0.000 claims abstract description 13
- 239000007788 liquid Substances 0.000 claims abstract description 10
- 239000000523 sample Substances 0.000 claims description 46
- 239000013068 control sample Substances 0.000 claims description 24
- 238000011109 contamination Methods 0.000 claims description 23
- 238000001514 detection method Methods 0.000 claims description 14
- 244000005700 microbiome Species 0.000 claims description 11
- 238000001914 filtration Methods 0.000 claims description 6
- 239000012982 microporous membrane Substances 0.000 claims description 6
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims description 6
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 claims description 3
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 claims description 3
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 229910052750 molybdenum Inorganic materials 0.000 claims description 3
- 239000011733 molybdenum Substances 0.000 claims description 3
- 229910052697 platinum Inorganic materials 0.000 claims description 3
- 229910052709 silver Inorganic materials 0.000 claims description 3
- 239000004332 silver Substances 0.000 claims description 3
- 229910001220 stainless steel Inorganic materials 0.000 claims description 3
- 239000010935 stainless steel Substances 0.000 claims description 3
- 229910052719 titanium Inorganic materials 0.000 claims description 3
- 239000010936 titanium Substances 0.000 claims description 3
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 claims description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 2
- 229910052804 chromium Inorganic materials 0.000 claims description 2
- 239000011651 chromium Substances 0.000 claims description 2
- 229910052802 copper Inorganic materials 0.000 claims description 2
- 239000010949 copper Substances 0.000 claims description 2
- 239000012895 dilution Substances 0.000 claims description 2
- 238000010790 dilution Methods 0.000 claims description 2
- 239000007769 metal material Substances 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 claims description 2
- 229910052725 zinc Inorganic materials 0.000 claims description 2
- 239000011701 zinc Substances 0.000 claims description 2
- 238000000053 physical method Methods 0.000 claims 3
- 229910052751 metal Inorganic materials 0.000 claims 2
- 239000002184 metal Substances 0.000 claims 2
- 229910052793 cadmium Inorganic materials 0.000 claims 1
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 claims 1
- 238000004140 cleaning Methods 0.000 claims 1
- 235000013361 beverage Nutrition 0.000 abstract description 12
- 241000894006 Bacteria Species 0.000 abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 7
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 235000020247 cow milk Nutrition 0.000 abstract description 2
- 238000009776 industrial production Methods 0.000 abstract description 2
- 230000035945 sensitivity Effects 0.000 abstract description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 235000019441 ethanol Nutrition 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 description 15
- 235000013405 beer Nutrition 0.000 description 12
- 238000005259 measurement Methods 0.000 description 9
- 239000010453 quartz Substances 0.000 description 7
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 7
- 150000001875 compounds Chemical class 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
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- 210000004080 milk Anatomy 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
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- 235000013305 food Nutrition 0.000 description 3
- 238000010166 immunofluorescence Methods 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 238000012856 packing Methods 0.000 description 3
- 238000005375 photometry Methods 0.000 description 3
- 239000008399 tap water Substances 0.000 description 3
- 235000020679 tap water Nutrition 0.000 description 3
- 238000010367 cloning Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 230000036962 time dependent Effects 0.000 description 2
- 101710160107 Outer membrane protein A Proteins 0.000 description 1
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 235000013409 condiments Nutrition 0.000 description 1
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- 238000011010 flushing procedure Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The present invention relates to a method for checking microbial pollution in liquid, particularly to a method for checking microbes in liquid, such as beverages, cow milk, alcohol, water, medicine, etc., by electric light emitting. For adapting the needs of industrial production, the method can be directly used for a production line of checked objects for producing and checking, so the work efficiency is enhanced, and the product quality is also ensured; thus, a superimposed voltage is supplied to be inserted on a couple of electrodes in a checked sample. Voltages are superimposed, and simultaneously, a single photon detector is used for measuring the change of the electric luminous intensity of a check sample and the checked sample following time. The polluted conditions of samples are obtained according to the difference of the average value of the luminous intensity. The method has the advantages of simplicity and quickness, and the high sensitivity reaches one hundred bacteria /ml.
Description
The present invention relates to the technical field of microbial contamination in a kind of detecting liquid, particularly utilize electroluminescence check beverage, milk, water, wine etc. or be made into the technical method that liquid is checked their central microbial contamination being verified solid.
Whether adopted cultural method in the check material at present commonly used by microbial contamination, i.e. cloning process, as check beer in process of production whether by bacterial contamination, milk in canned process whether by bacterial contamination or the like, cloning process such as document 1.Koch,A.:Crowth Measurement in Manualof methodsfor general bacteriology,ed by Gerhardt,Murray,Costilow,Nester,Wool,Krieg and Phillips American Society for Microbiology,1981 p179-206。This method is a kind of cultural method.Add in the nutrient culture media after at first will making different dilute samples to the sample that is verified, be placed on interior cultivation of incubator of proper temperature, general cultivation temperature is at 37 ℃, incubation time removes other material can cultivate several hrs, generally needs just can come out microorganism in the test sample or microbe growth after 24-48 hour.And then, observe its pollution condition that how much determines with clone's number of visual observations culture bacterium, and just think there is not bacterial contamination if can't see the clone, whole process needs carry out under aseptic condition.This method is because incubation time is long, cause long shortcoming of cycle of check, in food industry such as beer production, be unallowed often, Chang incubation time and influenced quality of beer so, cause economic loss, second this method accuracy of shortcoming is not high, and be sensitive inadequately, because the degree more than microorganism must reach necessarily in this method requirement test sample just can be seen draw, do not observe and mistaken diagnosis so when pollution condition is smaller, generally turn out bacterial clone.
Also has another fluorescent immunization method, as document 2.Wolff, L.F., Anderson, L., Sandberg, G.P., Reither, L., Binsfeld, C.A., Corinaldesi, G., Shelburne, C.E.Bacteria Concentration fluorescence immunoassay (BCFIA) for thedetection of periodontopathogens in plaque.J.Periodontol.1992,63.1093-101 described: it is used in some pharmaceutical sanitary field.By cultivating its immunofluorescence antibody, it just produces fluorescent with the immunofluorescence antibody of turning out to this method if in the detection thing microorganism is arranged, and by the fluorescent spectrophotometer measurement, just can observe the contaminated situation of fluorescence intensity judgement sample checking matter.The advantage of this method is fast, but the checkout procedure complexity, and apparatus expensive can not generally be promoted; Other shortcoming is only limited to specific bacterium, only be applicable to that promptly the bacterium of immunofluorescence is effective, thereby this method has limitation.
Also have a kind of in conjunction with measuring method, China as documents CN1064945A applies for disclosed method, it is the method and apparatus that generally carries out in conjunction with measuring, specifically relate to measure significant analyte by measuring by the one or more tagged compounds in the measuring system luminous, this method is to utilize the electrochemiluminescence routine techniques and improved in conjunction with assay method and device.Though the applicant utilizes this method by measuring cell surface antigen, detects the possibility of vestige microorganism, because these are many in conjunction with the method step of measuring, influence factor is many.Really be used for checking contamination by micro to be difficult to realize that this method does not provide embodiment simultaneously.
The objective of the invention is in order to overcome the shortcoming and defect of above-mentioned prior art, in order to adapt to industrial needs, can directly be used on beer, beverage, milk, the pharmaceutical manufacturing line and in the monitoring of water, the limit produces frontier inspection and tests the quality of enhancing productivity and guaranteeing product.Thereby providing a kind of powers up on a pair of telegram that is pressed in the measuring vessel that fills the fluid to be measured sample, switch on and cut off the power supply after several seconds, and limit making alive lateral dominance is measured the electroluminescence time-dependent variation in intensity of test sample and control sample with the single photon detection instrument, whether draws this test sample by the degree of microbial contamination and pollution according to the difference of luminous intensity.
The objective of the invention is to finish like this:
The first step of this method is ready to electrode, electrode commonly used is made thread, spiral fashion, plate-shaped electrode by copper, zinc, molybdenum, platinum, titanium, silver, chromium, tin, stainless steel and other metal materials, general wire electrode diameter is 0.2mm-1.0mm, and its length is 5mm-20mm; The plate-shaped electrode variable area is generally 1mm
2-100mm
2Electrode need thoroughly clean up before using, in case Clean belt does not pollute to sample.
Second step was prepared control sample and test sample
At first test sample is divided into two parts, (if solid sample must be made into fluid sample, because this method is only fitted and surveyed fluid sample), portion is sample in contrast, and is a as test sample.Control sample totally makes removal of microorganisms in the test sample through methods such as heating disinfection or filtering with microporous membrane with test sample, and is standby then; Another part test sample forms with the control sample dilution that performs, can be the two or more samples that contain the microorganism variable concentrations to the test sample dilution, and is standby respectively.
The 3rd pacing amount
Get the aseptic measuring vessel that cleans up, measuring vessel can be to make with transparent insulation materials such as quartz, glass.Pour into the control sample that prepared and the test sample of variable concentrations in the measuring vessel respectively, insert pair of electrodes again, distance is 1-30mm between two electrodes, measuring vessel is placed in the darkroom of single photon detection instrument, power source generator and two electrodes are linked, energized applies 5-100V voltage on electrode then, cut off the power supply after second 0.1 second-10 conduction time, can repeatedly repeat, and the electroluminescence intensity with single photon detection instrument measurement test sample and control sample compares in alive process, can judge whether bacterial contamination, further use the electroluminescence time-dependent variation in intensity and the control sample of test sample to compare again, by calculating luminous intensity to time integral, average relatively, obtain test sample by the degree of microbial contamination from mean value.
This method can be used for the industry of every needs check microbial contaminations such as the monitoring of beverage production, beer, milk, condiment, food, pharmacy, water.
Advantage of the present invention: method of the present invention is simple, need not pass through microbe culture.Need not use the induced luminescence tagged compound, need not get up to make to contain the compound of particle and described tagged compound, only need the electrifying electrodes in the test sample is produced photon, so this method be quick to triplicity.And launch with single photon detection instrument measurement of photon, the 3rd advantage is highly sensitive, can reach 100 bacterium/ml, therefore this method purposes is wide, can be used for the supervision of pollution of waterhead, beverage industry, brewing industry, cow's milk industry, food industry and medical industry etc., this method may be used on the scene, the single photon detection instrument directly is connected on the production line, and the limit produces frontier inspection and surveys.
Below in conjunction with drawings and Examples the present invention is described in detail;
Fig. 1 is the used measurement mechanism synoptic diagram of this method.
Fig. 2 is that this method is used in the synoptic diagram in the industrial production lines such as beer.
A kind of plate electrode synoptic diagram that Fig. 3 is in this method to be adopted.
Fig. 4 is a kind of control sample electroluminescence intensity and time relation curve of beverage.
Fig. 5 is that this kind beverage test sample (closes bacterial concentration and be 100/mL) electroluminescence intensity and time relation curve.
The drawing explanation:
1-electrode 2-electroplax
3-sample 4-measuring vessel
5-single photon detection instrument 6-power source generator
7-liquid-inlet 8-liquid outlet
Embodiment 1: utilize method of the present invention to measure bacterium in a kind of beverage at the single photon detection instrument.
This beverage adds bacterium in advance, and concentration is 100 bacterium/mL.Adopt the measurement mechanism of Fig. 1, molybdenum electrode 1,2 is a ∮ 0.2mm filament, and elder generation with the electrode wash clean, uses distilled water flushing with strong base solution again.Get quartzy measuring vessel strictness and clean up, tested beverage is poured into respectively in two quartz curettes, earlier one glass of beverage is cooked control sample with filtering with microporous membrane, get in another quartz curette beverage as test sample.Insert pair of electrodes respectively in measuring cup, its electrode separation is 5mm.On an electrode, apply the outage in 5 seconds of 30V voltage, be total to triplicate, and (Europe patent EP0439150 patent is produced, and to 800nm, sensitivity is 10 to this apparatus measures wavelength coverage at 200nm with the single photon detection instrument in pressure process
-17W), measure luminous intensity shown in Fig. 4,5, again electroluminescence intensity and the time integral that records calculated mean value, its result is as shown in following:
Bacterial population is (individual/mL) average electrical luminous intensity (photon/100ms).
0 75.5±7.5
100 54.8±10.1
Embodiment 2:
Utilize method of the present invention bacterial contamination degree in the instrument detecting beer of embodiment 1.
As shown in Figure 1,10mL beer packed into measure in the quartz curette as test sample, beer filtering with microporous membrane in another quartz curette, the control sample that removal of microorganisms in the test sample totally makes, standby then; Platinum electrode silk (∮ 0.5mm) insertion is filled in test sample and the control sample measuring cup, in the measurement darkroom with its single photon detection instrument of packing into, the sub-emissive porwer of photometry, making alive 50V, remove voltage 5 seconds, repeat once after 20 seconds, altogether triplicate, ask luminous intensity mean value, provide as table 1:
Embodiment 3: utilize method of the present invention bacterial contamination degree in the instrument detecting water of embodiment 1.
| Bacterial population (individual/mL) | Average canbdle power (photon/100ms) |
| 0 100 | 2850±101.9 2192±118.0 |
As shown in Figure 1,10mL distilled water packed into measure in the quartz curette as test sample, another is measured to pack in quartz curette to boil through heating and makes control sample, inserts silver electrode (∮ 0.5mm) in filling test sample and control sample measuring cup, in the measurement darkroom with its single photon detection instrument of packing into, the sub-emissive porwer of photometry, making alive 30V removes voltage 5 seconds, repeat once after 20 seconds, triplicate is asked luminous intensity mean value altogether, provides as table 3:
| Bacterial population (individual/mL) | Average canbdle power (photon/100ms) |
| 0 100 | 14.1±2.5 9.3±1.6 |
Embodiment 4: utilize method of the present invention bacterial contamination degree in the instrument detecting tap water of embodiment 1.
As shown in Figure 2, in two glass containers 4, charge into tap water, charge into the control sample that tap water totally makes removal of microorganisms in the test sample with filtering with microporous membrane in the glass container 4, standby then; Adopt stainless steel electrode, insertion fills in test sample and the control sample measuring cup, in the measurement darkroom with its single photon detection instrument of packing into, and the sub-emissive porwer of photometry, making alive 80V removes voltage 3 seconds, repeats secondary, asks luminous intensity mean value, provides as table 4;
| Bacterial population (individual/ml) | Average canbdle power (photon/1ms) |
| 0 100 | 125.6±26.8 80.7±13.3 |
Embodiment 5: utilize method of the present invention to use the instrument detecting beer of embodiment 1 by the degree of bacterial contamination.
As shown in Figure 3, a kind of beer is packed in the measuring cup, wherein a cup is gone in the measuring cup beer through filtering with microporous membrane, and is the control sample that removal of microorganisms in the test sample totally makes, standby then; Thick 0.1mm titanium plate electrode pack in control sample and test sample, the measuring cup that fills control sample and test sample is put into measured the darkroom, making alive 3V removes voltage 2 seconds, repeats secondary, surveys luminous intensity mean value, provides as table 5:
| Bacterial population (individual/ml) | Average canbdle power (photon/1ms) |
| 0 100 | 213.4±21.6 158.6±18.2 |
Claims (4)
1. method with microbial contamination in the electroluminescence quick test liquid, test sample is divided into two parts, the a processing through physical method made free of contamination control sample, another part made the test sample that contains different microorganisms concentration, control sample and test sample are poured into respectively in the clean measuring vessel (4) of cleaning, it is characterized in that: insert pair of metal electrodes (1), (2) in sample (3); Two electrodes (1), (2) distance is 1-30mm between, respectively the container that fills control sample and test sample is placed in the darkroom of single photon detection instrument (5) then, power source generator (6) and electrode (1), (2) link, in electrode (1), (2) apply 5-100V voltage on, the each energising once or repetition, each energising was cut off the power supply after 0.1 second-10 second, record the electroluminescence intensity of sample during energising with the single photon detection instrument, electroluminescence intensity by contrast test sample and control sample, electroluminescence intensity and time integral to recording again, the average canbdle power that calculates them compares, and obtains test sample by the degree of microbial contamination.
2. by the described method with microbial contamination in the electroluminescence quick test liquid of claim 1, it is characterized in that: described metal electrode (1), (2) are that to make wire electrode, the spiral fashion that φ 0.2-1.0mm * 5-20mm grows or make area with copper, zinc, molybdenum, platinum, titanium, silver, chromium, cadmium, stainless steel metal material be 1mm
2-1cm
2Plate-shaped electrode.
3. by the described method with microbial contamination in the electroluminescence quick test liquid of claim 1, it is characterized in that: described portion is handled through physical method and is made free of contamination control sample, is to adopt heating disinfection, or the physical method of filtering with microporous membrane.
4. by the described method with microbial contamination in the electroluminescence quick test liquid of claim 1, it is characterized in that: the test sample of described different microorganisms concentration is that the control sample dilution test sample of getting different volumes is made.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP19538768.6 | 1995-10-18 | ||
| DE19538768A DE19538768C2 (en) | 1995-10-18 | 1995-10-18 | Procedure for the detection of a microbial infection |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1150246A CN1150246A (en) | 1997-05-21 |
| CN1072799C true CN1072799C (en) | 2001-10-10 |
Family
ID=7775154
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 96105260 Expired - Fee Related CN1072799C (en) | 1995-10-18 | 1996-05-28 | Sensitive method for quickly testing microbe pollution by electroluminescence and its use |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN1072799C (en) |
| DE (1) | DE19538768C2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106085833A (en) * | 2016-05-31 | 2016-11-09 | 华北理工大学 | Microorganism metering device and metering method thereof in water |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4283490A (en) * | 1978-07-28 | 1981-08-11 | Plakas Chris J | Method for detection of low level bacterial concentration by luminescence |
| GB2073885B (en) * | 1980-04-15 | 1983-12-21 | Whitlock G D | Method of and apparatus for detecting the presence of live organisms in substances |
| DE3038255A1 (en) * | 1980-10-10 | 1982-05-19 | Wolfgang Prof. 7500 Karlsruhe Mehlhardt | Examining biological effects on foodstuffs of seeds - by measuring intensity of ultra-weak photon radiation in vitro |
| DE3040855A1 (en) * | 1980-10-30 | 1982-06-09 | Wolfgang Prof. 7500 Karlsruhe Mehlhardt | Examining biological effects on foodstuffs of seeds - by measuring intensity of ultra-weak photon radiation in vitro |
| DE3737649A1 (en) * | 1987-11-06 | 1989-05-24 | Inst Zellforschung Und Biolumi | Method for determining the luminescence of cell cultures, and device for carrying out the method |
| DE3935974A1 (en) * | 1989-10-28 | 1991-05-02 | Mueller Klieser Wolfgang Prof | Determn. of spatial distribution of metabolites in tissue samples - by bio-luminescence, using conc. viscous soln. of luciferase and counting photons in unit area |
| DE3939411A1 (en) * | 1989-11-29 | 1991-06-06 | Popp Fritz Albert | METHOD FOR THE EXAMINATION OF THE QUALITY AND QUALITY CHANGES OF BIOLOGICAL SYSTEMS AND INTERACTIVE ORGANIC CHEMICAL COMPOUNDS THEREOF BY MEASUREMENT OF THE ULTRA-CHANGING PHOTON EMISSION |
| DE4308520A1 (en) * | 1993-03-17 | 1994-09-22 | Popp Fritz Albert Dr | Method for differentiating between homozygotes, heterozygotes and normal cells of an organism |
| DE4401169C2 (en) * | 1994-01-17 | 2003-01-09 | Buehler Ag | Process for distinguishing the quality of liquids |
| DE9417845U1 (en) * | 1994-11-08 | 1995-04-20 | Popp, Fritz-Albert, Dr., 67661 Kaiserslautern | System for measuring ultra-weak photon emission (photon measuring system) |
-
1995
- 1995-10-18 DE DE19538768A patent/DE19538768C2/en not_active Expired - Fee Related
-
1996
- 1996-05-28 CN CN 96105260 patent/CN1072799C/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106085833A (en) * | 2016-05-31 | 2016-11-09 | 华北理工大学 | Microorganism metering device and metering method thereof in water |
| CN106085833B (en) * | 2016-05-31 | 2018-03-13 | 华北理工大学 | Microorganism metering device and its metering method in water |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1150246A (en) | 1997-05-21 |
| DE19538768A1 (en) | 1997-04-24 |
| DE19538768C2 (en) | 2003-02-27 |
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